Dispenser system, test kit, and method of adding reagent using the dispenser system

Abstract

To provide a dispenser system capable of adding a small amount of reagent for point-of-care testing (POCT).SOLUTION: A dispenser system (1) provided herein allows a reagent or sample to be introduced into an introduction section (2) having an internal space (22) facing open upper ends of capillary parts (10), and a loading section (27), loaded with a fiber product (70) capable of absorbing excess reagent or sample remaining in the internal space (22) when a reagent or sample is sucked into the capillary parts (10), is provided on the outside of the internal space (22) in the introduction section (2).SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a dispenser system for adding a reagent, a test kit including the dispenser system, and a method for adding a reagent using the dispenser system. [Background technology]

[0002] Point-of-care testing (POCT) is a portable test performed on-site, unlike traditional tests performed in hospitals and other facilities. Demand for POCT is increasing. Its applications are diverse, including early infection diagnosis and rapid isolation using portable devices for viral infection testing, and rapid drug contamination testing at disaster sites using biomimetic portable devices. A biomimetic portable device is an in vitro culture system that recreates a culture environment similar to that of a living organism (in vivo) in a microspace created using microfabrication technologies such as MEMS (micro electro mechanical systems).

[0003] To be portable, POCT devices are necessarily small, and to carry out reactions within them, a dispenser system that allows for the manipulation of small amounts of reagents at the sub-microliter (1 / 10,000th of a milliliter) scale is essential.

[0004] A conventional example of a dispenser system is the dispenser system disclosed in Patent Document 1. Although this system is capable of handling small amounts of reagents, it requires electrical control and is expensive to manufacture, making it difficult to use as a practical dispenser system for POCT.

[0005] Furthermore, for example, the multi-channel dispenser of Patent Document 2 has a complicated mechanism for adjusting the amount of liquid, which makes the dispenser large and makes it unsuitable for adding reagents in a practical POCT dispenser system.

[0006] The present inventors have also proposed a dispenser for handling small amounts of reagents, as shown in Non-Patent Document 1. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] Patent No. 7333319 [Patent Document 2] Japanese Patent Publication No. 2022-013767 [Non-patent literature]

[0008] [Non-Patent Document 1] Yusuke Kimura, et al. “Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP”, Conf Proc IEEE Micro Electro Mechanical Systems, (2019). Summary of the Invention [Problem to be solved by the invention]

[0009] There is room for improvement in terms of practicality in Non-Patent Document 1. For example, Non-Patent Document 1 uses sodium polyacrylate for water absorption, which not only increases the cost of producing the dispenser but also poses the problem that sodium polyacrylate swells when absorbing water, resulting in a small amount that can be installed in the device, which significantly limits the amount of reagent that can be manipulated.

[0010] In light of the above, the present inventors have continued research and development into a dispenser with superior practicality, and have completed the present invention.

[0011] An object of one aspect of the present invention is to provide a practical dispenser system that can be used for adding small amounts of reagents for POCT. [Means for solving the problem]

[0012] In order to solve the above problems, one aspect of the present invention provides a dispenser system having an internal space with an open upper end of a capillary section that can suck in a certain amount of reagent or sample by capillary action, an introduction section that can introduce the reagent or sample into the internal space from an upper inlet, and a pressure mechanism that can pressurize the internal space in the introduction section, and is configured so that the reagent or sample sucked into the capillary section is added to a target location by pressurizing the internal space with the pressure mechanism, and a loading section that is loaded with a processed fiber material that can absorb excess reagent or excess sample remaining in the internal space when the reagent or sample has been sucked into the capillary section is provided outside the internal space in the introduction section.

[0013] In order to solve the above problems, a test kit according to one embodiment of the present invention comprises the above-mentioned dispenser system and a test device that can be equipped with the dispenser system and performs a test using the reagent or sample added from the dispenser system.

[0014] In order to solve the above problems, a reagent addition method according to one aspect of the present invention is a reagent addition method using the above-mentioned dispenser system, and includes a preparation step of introducing the reagent or sample from the inlet into the internal space of the introduction section, and after the reagent or sample has been sucked into the capillary section, allowing the textile processed product to absorb any excess reagent or excess sample remaining in the internal space, and an addition step of adding the reagent or sample sucked into the capillary section to a target location by pressurizing the internal space using the pressure mechanism after the preparation step. [Effects of the Invention]

[0015] According to one aspect of the present invention, a practical dispenser system that can be used to add small amounts of reagents for POCT can be realized. [Brief explanation of the drawings]

[0016] [Figure 1] 1 shows a dispenser system according to one embodiment of the present invention, with the center being a front view of the dispenser system, the upper side being a top view of the dispenser system, and the lower side being a bottom view of the dispenser system. [Figure 2] FIG. 2 is a perspective view of the dispenser system of FIG. 1. [Figure 3] 2 is a perspective view of the dispenser system of FIG. 1 as viewed from above. [Figure 4] 2 is a top perspective view illustrating the positional relationship between a capillary portion and an internal space of an introduction portion of the dispenser system of FIG. 1. FIG. [Figure 5] 2 is a top view showing a state in which a fiber workpiece is loaded into a loading section of an introduction section of the dispenser system of FIG. 1. FIG. [Figure 6] 2 is a perspective view showing a state in which a fiber product is loaded into a loading section of an introduction section of the dispenser system of FIG. 1. FIG. [Figure 7] FIG. 2 is a front perspective view of the dispenser system of FIG. 1 including a cap portion. [Figure 8] FIG. 2 is a diagram illustrating a process flow of a reagent adding method using the dispenser system of FIG. [Figure 9] 9 is a diagram illustrating a reagent addition procedure using the dispenser system of FIG. 1 in accordance with the processing flow shown in FIG. 8. FIG. [Figure 10] FIG. 2 shows a test kit according to one embodiment of the present invention using the dispenser system of FIG. 1. [Figure 11] 11 is a diagram schematically showing a state in which a reagent is added to a well of a reactor chip in the test kit of FIG. 10. FIG. [Figure 12] FIG. 12 is a diagram schematically showing the configuration of a testing device that performs testing using the reactor chip of FIG. [Figure 13] FIG. 10 is a front perspective view showing a dispenser system according to another embodiment of the present invention. [Figure 14]14 is a diagram illustrating a liquid delivery mechanism of the dispenser system shown in FIG. 13. FIG. [Figure 15] FIG. 14 is a diagram showing a test kit according to another embodiment of the present invention, which uses the dispenser system shown in FIG. 13. [Figure 16] FIG. 1 is a diagram showing the results of an example. DETAILED DESCRIPTION OF THE INVENTION

[0017] [Embodiment 1] Hereinafter, one embodiment of a dispenser system according to one aspect of the present invention will be described. The dispenser system of this embodiment can simultaneously add a fixed amount of reagent from each of a plurality of capillary portions. Hereinafter, the dispenser system of this embodiment uses a reagent (additive reagent) that is added in a specified amount from each capillary portion, and a pushing reagent for pushing this additive reagent out of the capillary portion when adding this additive reagent. Below, the additive reagent and the pushing reagent will be described, but the present invention is not limited to these.

[0018] <Additive reagents> The term "reagent" as used herein refers to an additive reagent. The additive reagent may be any liquid that can be drawn into the capillary portion 10 by capillary action and that can be added to a target location from the capillary portion 10 by a method described below. The additive reagent may be a polar solvent (aqueous solution) or a non-polar one.

[0019] Examples of polar solvents (aqueous solutions) include aqueous reagents containing a single polar substance, such as saline, and aqueous reagents composed of multiple substances, such as PCR (Polymerase Chain Reaction) reagents and LAMP (Loop-mediated isothermal amplification) reagents. They also include pure water and other polar solvents.

[0020] The non-polar substance may be an oil, which may be a liquid non-polar solvent or a fat-soluble reagent containing a fat-soluble component at the temperature of the environment in which the dispenser system of this embodiment is used.

[0021] The added reagent may be a specimen sample used in a viral gene detection experiment using the LAMP method.

[0022] <Extrusion Reagent> When the additive reagent is a polar solvent (aqueous solution) as described above, the extrusion reagent can be oil, which is a liquid non-polar solvent or a fat-soluble reagent containing a fat-soluble component at the temperature of the environment in which the dispenser system of this embodiment is used, and includes mineral oil, silicone oil, rapeseed oil, benzene, etc.

[0023] On the other hand, when the added reagent is non-polar, a polar solvent (aqueous solution) can be used as the pushing reagent. The polar solvents (aqueous solutions) exemplified above can be used as the pushing reagent.

[0024] The dispenser system of this embodiment will be described below with reference to FIGS. 1 to 7. The reagent addition method of this embodiment will be described with reference to FIGS. 8 to 9. The test kit of this embodiment will be described with reference to FIGS. 10 to 12. In the drawings, the XYZ three-dimensional coordinate system, in which the vertical direction is the Z axis and the horizontal plane is the XY plane, is also shown, and the arrangement and positional relationship of each component in the dispenser system and test kit of this embodiment will be described. In the following, the arrangement and positional relationship of the components in the dispenser system of this embodiment will be described assuming that the central axis direction of the capillary part is approximately parallel to the Z axis.

[0025] [1] Dispenser system In Fig. 1, a side (front) view of the dispenser system 1 of this embodiment is shown in the center B of the figure, a top view of the dispenser system 1 is shown in the upper side A of the figure, and a bottom view of the dispenser system 1 is shown in the lower side C of the figure. In Fig. 2, the left side A of the figure shows a perspective view of the dispenser system 1 as seen from the front, and the right side B of the figure is an exploded perspective view showing the dispenser system 1 shown in the left side A of the figure in an exploded state.

[0026] The dispenser system 1 includes a capillary portion 10 that can suck in a predetermined amount of reagent (hereinafter simply referred to as "reagent") as the aforementioned additive reagent by capillary action. In this embodiment, a plurality of capillary portions 10 are provided, but only one capillary portion 10 may be provided. Each capillary portion 10 has an internal volume that can dispense a predetermined amount of reagent, and in one example, has an internal volume that can dispense a reagent on a sub-microliter scale. Note that each capillary portion 10 may have an internal volume that can dispense a reagent on a nanoliter or microliter scale, not limited to a sub-microliter scale.

[0027] The dispenser system 1 also includes an introduction section 2 above the capillary section 10, the introduction section 2 having an introduction main body section 20 and an inlet .

[0028] The introduction part 2 has an internal space 22 in which the upper end 10a of the capillary part 10 is open at the bottom (FIG. 2). The introduction part 2 is configured by combining an introduction main body part 20 provided with a configuration on the bottom side of the internal space 22 and an inlet 26 provided with a configuration on the top side of the internal space 22, and the internal space 22 is realized by combining them.

[0029] 2, the inlet 26 has a structure that covers the upper end of the introduction body 20, and the inlet 26 and the introduction body 20 are fixed by adhesive. A flow path is provided in the upper part of the inlet 26, and the flow path communicates with the internal space 22 at the lower part. An inlet 26a corresponding to the upper end of the flow path is provided in the upper part of the inlet 26. As will be described later, a reagent is introduced into the inlet 26a using, for example, a micropipette.

[0030] When adding a reagent to a target location from each capillary portion 10 in the dispenser system 1, a cap portion 40 (see FIG. 7, described later) is attached to the upper portion of the inlet 26 on the inlet 26a side. The cap portion 40 has a hole 43 on its top surface, which serves to release air from a cavity 41 when the cap portion 40 is attached to the inlet 26. This prevents the air in the cavity 41 from pushing out the sample in the dispenser system 1 during attachment. The cap portion 40 (pressurizing mechanism) has a cavity 41, and the internal volume of the cavity 41 changes when the cap portion 40 is pressed from the outside. When the cap portion 40 is pressed, the cavity 41 is crushed, and the air in the cavity 41 is pushed out toward the flow path of the inlet 26. This air pressure causes the reagent dispensed into the capillary portion 10 to be discharged from the lower end of the capillary portion 10, as described later.

[0031] 3 is a perspective view of the inlet 26, viewed from the same direction as the top view shown at the top of FIG. 1. The inlet 26 has a lower opening 26b, which forms the lower end of a flow path having an inlet 26a at its upper end, and eight recessed grooves 260a-260h, on its surface (hereinafter referred to as the lower surface) that forms the ceiling side of the internal space 22. By combining the inlet 26 with the introduction main body 20, a flow path is formed, surrounded by the bottom surface of the internal space 22 formed in the introduction main body 20 and the inner surfaces of each of the recessed grooves 260a-260h. In other words, the internal space 22 can be described as flow paths extending radially from the lower opening 26b, which is located at the center in the top view. A reagent introduced from the inlet 26a of the inlet 26 flows into the internal space 22, which is made up of these eight flow paths.

[0032] 4 shows the relationship between the positions of the eight flow paths formed by the eight grooves 260a-260h and the positions of the upper ends 10a of the capillary portions 10. As shown in FIG. 4, the upper ends 10a of the capillary portions 10 are located in any of the eight flow paths formed by the grooves 260a-260h. Specifically, using the example shown in FIG. 4, of the nine capillary portions 10 arranged 3×3 in the vertical (Y-axis direction) and horizontal (X-axis) directions on the paper surface of FIG. 4, the upper end 10a of the central capillary portion 10 is located opposite the lower opening 26b of the inlet 26, and the upper ends 10a of the remaining eight capillary portions 10 are located opposite each of the eight grooves 260a-260h. In this way, the upper ends 10a of the capillary portions 10 are positioned in the eight flow paths formed by the recessed grooves 260a to 260h, so that the reagent is efficiently sucked into the capillary portions 10.

[0033] Although the present embodiment shows an example in which eight grooves 260a-260h are provided extending in the radial direction, the total number, positions, and extension directions of the grooves are not limited to the configuration of the present embodiment and may be designed, for example, in relation to the number and arrangement positions of the capillary portions 10. Furthermore, in the present embodiment, the lower opening 26b is provided at the center of the inlet 26 in a top view, but this is not limiting, and the number of lower openings 26b of the inlet 26 is not limited to one. Therefore, the total number and arrangement of grooves in the internal space 22 may be designed appropriately depending on the positions and number of lower openings 26b of the inlet 26.

[0034] Next, the introduction main body 20, which is provided with a recessed structure that forms the bottom side of the internal space 22, will be further described with reference to FIG. 5. FIG. 5 is a top view of the introduction main body 20, on the side where the recessed structure that forms the bottom side of the internal space 22 is provided. As shown in FIG. 5, a loading section 27 is provided on the outside of the recessed structure that forms the bottom side of the internal space 22 in the introduction main body 20. The loading section 27 is loaded with a fiber processed product 70 that can absorb excess reagent (excess sample if the added reagent is a specimen sample) remaining in the internal space 22 when the reagent (added reagent) is aspirated into the capillary portion 10. In other words, the loading section 27 is configured to be provided outside the internal space 22 in the introduction section 2. Note that, hereinafter, in describing the arrangement of the loading section 27, the recessed structure that forms the bottom side of the internal space 22 in the introduction main body 20 will be referred to as the internal space 22.

[0035] As shown in Figure 5, the loading section 27 is arranged to surround the internal space 22 when viewed from above, and the loading section 27 and the internal space 22 are separated by a partition section 28 which has a communication hole 80 that connects the loading section 27 and the internal space 22 in part.

[0036] There are multiple communication holes 80, which are arranged symmetrically around the internal space 22. This allows excess reagent to be absorbed into the processed fiber product 70 from multiple locations, making it difficult for the reagent to remain in the internal space 22.

[0037] Specifically, a plurality of loading sections 27 are arranged side by side so as to surround the internal space 22. In this embodiment, four loading sections 27 are arranged along the periphery of the internal space 22, which is rectangular in top view, and adjacent loading sections 27 are separated by partial partitions 28a that are part of the partitions 28. Each loading section 27 communicates with the internal space 22 via communication holes 80 provided in the partitions 28 in the four corner regions of the rectangular internal space 22 in top view. The processed fiber material 70 is loaded as an independent configuration into each loading section 27.

[0038] The fiber product 70 will be described with reference to Fig. 6. Fig. 6 is a perspective view of the internal space 22 as viewed obliquely from above. As shown in Fig. 6, the fiber product 70 arranged in each loading section 27 is in the form of a strip (band), and is arranged along the periphery of the internal space 22 between one partial partition body 28a and the other partial partition body 28a.

[0039] The processed fiber product 70 has the property of absorbing excess reagent in the internal space 22. More specifically, the processed fiber product 70 only needs to have the property of being able to absorb excess reagent and not dissolving undesirable substances due to the absorbed excess reagent. Undesirable substances are substances that may dissolve in the reagent dispensed from the capillary portion 10 and adversely affect the test or the like performed after the dispense.

[0040] Furthermore, it is preferable that the processed fiber product 70 has a low swelling property, that is, it has a property that when it absorbs excess reagent, it does not easily increase in volume compared to before absorption.

[0041] As an example, Japanese paper can be used as the processed fiber product 70. Japanese paper is suitable for use because it is strong due to the long intertwined fibers made from bark and can prevent excess reagent from flowing back after absorption. However, the processed fiber product 70 is not limited to Japanese paper. However, it is preferable that the processed fiber product 70 be able to maintain a rectangular shape so that it can be placed in the loading section 27, be liquid-absorbent, and have little flow-back of absorbed liquid. Specifically, in addition to Japanese paper, cloth (liquid-absorbent cloth) or paper rags can be used.

[0042] For example, the processed fiber product 70 may have an absorption capacity that corresponds to the size of the processed fiber product 70. The processed fiber product 70 may be placed in the loading section 27 with a size that can absorb the entire amount of excess reagent. Note that the extrusion reagent (mineral oil) described above has a different polarity from the excess reagent, and therefore the processed fiber product 70 does not absorb mineral oil. Note that the processed fiber product 70 does not change even when it comes into contact with mineral oil, and does not allow the absorbed reagent to return.

[0043] Here, a structure is provided on the bottom side of the internal space 22 of the introduction main body 20 that allows excess reagent to easily come into contact with the fiber product 70. As an example, it is preferable that, of the eight grooves 260a to 260h (FIG. 4) described above, each communication hole 80 is located at the extending end of grooves 260b, 260d, 260f, and 260h that extend toward the corner regions of the internal space 22, which is rectangular in top view. This allows the reagent to be supplied to the capillary portion 10 through grooves 260b, 260d, 260f, and 260h, and any excess reagent that remains without being sucked into the capillary portion 10 passes directly through grooves 260b, 260d, 260f, and 260h to the extending end and is absorbed into the fiber product 70 via the communication hole 80. 5 and 6, an inclined portion 29 is provided at the bottom of each communication hole 80 to prevent the reagent from coming into contact with the textile product 70 before it has contacted the upper ends of all the capillaries. In the example of FIG. 6, the inclined portion 29 slopes upward from the bottom surface of the internal space 22 toward the loading portion 27. This structure allows excess reagent to be temporarily retained within the internal space, allowing the reagent to be introduced into all the installed capillaries.

[0044] FIG. 7 is a diagram showing the overall configuration of the dispenser system 1. As described above, the dispenser system 1 allows the cap portion 40 to be attached to the top of the inlet 26. In other words, the cap portion 40 is removably provided in the dispenser system 1. Addition of a reagent from each capillary portion 10 to a target location is performed with the cap portion 40 attached to the top of the inlet 26. Specifically, the cap portion 40 attached to the top of the inlet 26 is crushed to push the air in the cavity portion 41 toward the flow path of the inlet 26. This air pressure causes the reagent dispensed into the capillary portion 10 to be discharged from the bottom end of the capillary portion 10.

[0045] Here, in a state where the reagent is sucked into the capillary portion 10 and the excess reagent remaining in the internal space 22 is absorbed by the textile product 70, mineral oil is introduced into the internal space 22 as the extrusion reagent described above. The method for introducing the mineral oil into the internal space 22 may be the same as the method for introducing the reagent (additional reagent) into the internal space 22. With the mineral oil introduced into the internal space 22, the cap portion 40 attached to the top of the inlet 26 is pressed, thereby converting the air pressure of the cap portion 40 into hydraulic pressure, and the reagent sucked into the capillary portion 10 is added to the target location.

[0046] The dispenser system 1 further includes a support part 30 that supports the capillary part 10. The support part 30 supports the capillary part 10. As a result, even if the capillary part 10 is distorted when fixed to the bottom surface side of the introduction body 20, the reagent addition position does not deviate from the designed position, and the reagent can be accurately added to the target location from the capillary part 10.

[0047] <Method of manufacturing dispenser system 1> As an example of a method for manufacturing the dispenser system 1 of this embodiment, the introduction main body portion 20, inlet 26, support part 30, and cap portion 40 are each formed using a 3D printer, the glass capillary portion 10 is mounted on the formed support part 30, the fiber processed product 70 is mounted on the loading portion 27 of the formed introduction main body portion 20, and the respective parts are combined.

[0048] Each part can be made using a material appropriate for the intended use of the dispenser system 1. Disposable dispensers can be realized by using inexpensive materials that do not affect the reagents. Furthermore, further cost reductions can be achieved by using a 3D printer to print each part. The introduction body 20, inlet 26, and support part 30 can be made using, for example, epoxy-based photocurable resin, ABS resin, PLA resin, etc. These resins are effective when used with stereolithography (SLA, LCD, DLP, etc.) and injection molding (FDM) 3D printers or molding methods. The cap 40 can be made of soft materials such as rubber-like resin and silicone rubber.

[0049] The surfaces of the introduction body 20 and the inlet 26 that come into contact with the reagent are treated with a hydrophilic anti-adsorption coating or other surface treatment to prevent adsorption of nucleic acids and proteins in the sample or reagent. Furthermore, the triangular areas between adjacent grooves 260a-260h in Fig. 3, which are triangular in plan view, are treated with a hydrophobic coating. This allows the reagent to flow efficiently into the grooves 260a-260h.

[0050] [2] Reagent addition method using a dispenser system The reagent adding method of this embodiment is a reagent adding method using a dispenser system 1. Fig. 8 is a diagram illustrating the operation flow of this reagent adding method. Fig. 9 is a cross-sectional view of the dispenser system 1 corresponding to the operation flow of the reagent adding method of Fig. 8.

[0051] As shown in FIG. 8, the reagent addition method S1 includes a preparation step S11, an oil introduction step S21, and an addition step S31.

[0052] In preparation step S11, reagent L1 (additive reagent) is introduced from the inlet 26a of the inlet 26 ((i) in FIG. 9). A portion of the introduced reagent is sucked from the internal space 22 into the capillary portion 10 ((ii) in FIG. 9), and the excess reagent remaining in the internal space 22 is absorbed by the textile product 70 ((iii) in FIG. 9).

[0053] The oil introducing step S21 is a step between the preparation step S11 and the adding step S31, which will be described later. In the oil introducing step S21, mineral oil is introduced into the internal space 22 from the entrance 26a of the inlet 26. The mineral oil introduced into the internal space 22 fills the internal space 22 and comes into contact with the liquid surface of the reagent exposed toward the internal space 22 at the upper end 10a of the capillary portion 10.

[0054] In the adding step S31, after the oil introducing step S21, the cap portion 40 is fitted ((iv) of FIG. 9), and the reagent aspirated into the capillary portion 10 in the preparation step S11 is added to the target location by pressing the cap portion 40 arranged above the introducing portion 2 from the outside ((v) of FIG. 9). The mineral oil filled in the internal space 22 is hydraulically converted by the air pressure of the cap portion 40 being sent to the internal space 22, and a certain amount of the reagent aspirated into the capillary portion 10 is added simultaneously from each capillary portion 10.

[0055] Alternatively, the oil introduction step S21 may be omitted, and the addition step S31 may be performed following the preparation step S11, so that the reagent (added reagent) sucked into the capillary portion 10 is added to the target location using only the air pressure of the cap portion 40 without being pushed out using mineral oil. Alternatively, the cap portion may be mounted from the start of S1, and the reagent and oil may be added through the hole 43 in the upper part of the cap portion 40.

[0056] As described above, the dispenser system 1 of this embodiment is configured to absorb excess reagent using a processed fiber material, and therefore can be manufactured at a lower cost than conventional dispensers that use sodium polyacrylate to absorb water.

[0057] Furthermore, because sodium polyacrylate swells when it absorbs water, the amount that can be loaded onto the device is small, which presents a problem of significantly limiting the amount of reagent that can be used. In contrast, the dispenser system 1 of this embodiment does not use sodium polyacrylate, but is configured to absorb excess reagent using a processed fiber material, thereby alleviating the restrictions on the amount of reagent that can be used compared to conventional methods. Furthermore, the dispenser can be driven by itself. This allows not only those familiar with the operation, such as medical professionals, but also those unfamiliar with the operation, to dispense a predetermined amount of reagent.

[0058] Furthermore, the operation of dispensing the additive reagent using the dispenser system 1 is a simple operation, and has the advantage that even a person unfamiliar with the operation can operate it.

[0059] Furthermore, as described above, the dispenser system 1 can dispense a reagent by pressing the cap portion 40, and the dispenser can be driven without using electrical control. In this way, the dispenser system 1 can be used in non-electrical environments, and can be used outdoors or in disaster areas, for example.

[0060] As described above, according to this embodiment, a practical dispenser system that can be used to add a small amount of reagent for POCT can be realized.

[0061] Furthermore, by providing multiple capillary portions 10 as in this embodiment, it is possible to simultaneously add multiple submicroliter-scale samples with a very simple and easy operation of just pushing them in. This makes it possible to simultaneously test multiple virus species, drug contamination, etc. in a single test on a portable device.

[0062] [3] Test kit An embodiment of a test kit according to one aspect of the present invention will be described with reference to FIG. 10. FIG. 10 is an exploded view showing the configuration of test kit 100 of this embodiment. Test kit 100 of this embodiment is, as an example, a device for simple and early detection of viral infection via the loop-mediated isothermal amplification (LAMP) method. The test kit can simultaneously test for nine types of viral infection by observing fluorescence intensity with the naked eye or using a smartphone camera.

[0063] 10 is a palm-sized portable microdevice. The test kit 100 includes the above-mentioned dispenser system 1 and a test device 300 that can be equipped with the dispenser system 1 and performs a test using a reagent added from the dispenser system 1.

[0064] The testing device 300 includes a holder 301 , a reactor chip 302 , and a heating vessel 303 .

[0065] In test kit 100, with at least dispenser system 1 and reactor chip 302 mounted on holder 301, reagent (additional reagent) is dispensed from dispenser system 1 into the wells of reactor chip 302. Figure 11 is a diagram showing this process in schematic form.

[0066] As shown in the upper side A of Figure 11, a specimen sample L1, which is an example of an added reagent, is pushed downward from the capillary portion 10 of the dispenser system 1 by mineral oil L2 (pushing reagent) and dispensed into each well 302a of the reactor chip 302.

[0067] In the reactor chip 302 shown in the lower B of Figure 11, a specimen sample is dispensed into each well 302a, and mineral oil is also added. In a configuration in which the mineral oil does not affect the test, an extrusion reagent is added to the reactor chip 302 along with the additive reagent. As an example, the reactor chip 302 may be a container that is 7 mm square when viewed from above and 1.5 mm deep, with a total of nine wells 302a provided on the bottom surface, for example, three vertically and three horizontally, corresponding to the number of capillary portions 10. The diameter of each well 302a is, for example, 0.8 mm.

[0068] 12 is a diagram showing a schematic diagram of a test using the test kit 100. As described above, the reactor chip 302, into which the specimen sample has been dispensed into each well 302a, is heated using molten palmitic acid sealed in the heating container 303. While all of the palmitic acid in the heating container 303 transitions from liquid to solid, it is maintained at its melting point of 62.9°C, and the LAMP method is carried out using this.

[0069] After the reaction, the heating vessel 303 is removed, and optical filters 401a and 401b, such as bandpass filters, and a light source part 402, such as an LED, are installed. As an example, the light source part 402, which is equipped with an LED that emits light with a wavelength of 470 nm, is turned on. The light from the light source part 402 passes through a collimator lens 403 and optical filter 401a located above the light source part 402, and is then irradiated onto the reactor chip 302. Fluorescence with a wavelength of 520 nm excited by the irradiation travels upward.

[0070] 12, a bandpass filter 401b is disposed above the reactor chip 302, and light transmitted through the bandpass filter 401b travels upward. In the example of FIG. 12, light transmitted through the bandpass filter 401b and the macro lens 406 is captured by an imaging device 400 disposed above the test kit 100. As an example, the imaging device 400 may be a camera mounted on a smartphone. Note that the naked eye of an inspector may be used instead of the imaging device 400.

[0071] The bandpass filter 401b and the macrolens 406 can be mounted on the above-mentioned holder 301. As an example, after dispensing a reagent (additive reagent) from the dispenser system 1 into a well of the reactor chip 302, the dispenser system 1 is removed from the holder 301, and instead the bandpass filter 401b and the macrolens 406 are attached to the holder 301. Then, the holder 301 is installed in an optical device 405 on which a light source part 402 and a collimator lens 403 are mounted.

[0072] Although the test kit 100 of this embodiment is a device for simple and early detection of viral infection via the LAMP method, the type of test kit is not limited to this. Furthermore, the term "test" is not limited to this, and any processing kit that requires the dispenser system 1 to dispense a reagent (additive reagent) is included in the test kit of one embodiment of the present invention. For example, kits for nucleic acid analysis, protein analysis using antigen-antibody reactions, and sequence analysis using a sequencer are possible. Examples of nucleic acid analysis include PCR, real-time PCR, qRT-PCR, LAMP, SmartAmp, strand displacement amplification (SDA), recombinase polymerase amplification (RPA), and agarose gel electrophoresis. Examples of protein analysis using antigen-antibody reactions include ELISA. Examples of sequence analysis include the Sanger method and next-generation sequencing (NGS).

[0073] The dispenser system of this embodiment employs a pressurizing mechanism that applies pressure to the cap portion as a mechanism for pushing out the reagent from the capillary portion 10. However, the dispenser system of one aspect of the present invention is not limited to this. For example, a battery-powered pressurizing mechanism may be employed to push out the reagent (additional reagent and extrusion reagent) from the capillary portion 10. In the case of a battery-powered system, it is sufficient to add a configuration for mounting a battery, and the dispenser system does not become larger, and can be realized as portable as a dispenser system employing a cap portion.

[0074] [Embodiment 2] Other embodiments of the present invention will be described below. For ease of explanation, the same reference numerals will be used to designate components having the same functions as those described in the above embodiment, and the description thereof will not be repeated.

[0075] [1] Dispenser system The dispenser system of this embodiment differs from the dispenser system 1 of embodiment 1 in the manner in which the reagent is introduced into the internal space of the introduction part, specifically in the configuration of the inlet.

[0076] Fig. 13 is a perspective side view of dispenser system 1A of the present embodiment, and is a view corresponding to the left side view in Fig. 2 described in embodiment 1. As shown in Fig. 13, dispenser system 1A is provided with an inlet 26A instead of inlet 26 of embodiment 1.

[0077] The inlet 26A includes a connector lower part 261 having a structure that covers the upper part of the introduction main body part 20, and a liquid supply pillar 262 that is located above the connector lower part 261. The connector lower part 261 and the liquid supply pillar 262 both have flow paths that communicate with each other, and these flow paths communicate with the internal space 22.

[0078] Here, in the dispenser system 1 of the first embodiment, a micropipette or the like is used to introduce the reagent (additional reagent, extrusion reagent) into the entrance 26a of the inlet 26. In contrast, in the dispenser system 1A, a reagent introduction reactor and a reagent introduction holder are used to introduce the reagent (additional reagent, extrusion reagent) into the inlet 26A. For this reason, the dispenser system 1A is equipped with a reagent introduction reactor and a reagent introduction holder. The reagent introduction reactor and the reagent introduction holder will be described below with reference to FIG. 14.

[0079] Fig. 14 is a diagram illustrating a mechanism for introducing a reagent into the inlet 26A using a reagent introduction reactor 800 and a reagent introduction holder 900. The left side A of Fig. 14 shows the state before the reagent is introduced, and the right side B of the drawing shows the state after the reagent has been introduced.

[0080] The reagent introduction holder 900 is provided with a hole 901 through which the liquid supply pillar 262 can be inserted. An opening 902 for installing the reagent introduction reactor 800 is provided at the top of the reagent introduction holder 900. The left side A of FIG. 14 shows a state in which the reagent introduction holder 900 and the inlet 26A are not connected. From the state shown in the left side A of FIG. 14, the reagent introduction holder 900 is lowered in the negative direction of the Z axis toward the inlet 26A, as indicated by the arrow in the figure. The lowering operation can be performed manually. By lowering the reagent introduction holder 900, the top of the liquid supply pillar 262 inserted into the hole 901 penetrates toward the opening 902, as shown in the right side B of FIG. 14.

[0081] 14, a configuration is provided in which a reagent introduction reactor 800 is installed in the opening 902. The reagent introduction reactor 800 can be inserted into the opening 902 from the outside.

[0082] The reagent introduction reactor 800 has a bottom surface 801 provided with a hole through which the solution supply pillar 262 can be inserted, and a lid 803 provided at the top to close the hole, with the lid 803 opening toward the inside of the reagent introduction reactor 800. When the lid 803 is opened, the inside and outside of the reagent introduction reactor 800 can be communicated through the opening area. In this embodiment, the lid 803 is opened by the upper end of the solution supply pillar 262 pushing upward from the bottom side of the opening 902 as described above. In other words, when the upper end of the solution supply pillar 262 pushes upward from the bottom side of the opening 902, the lid 803 is pushed up and opened, and is inserted into the reagent introduction reactor 800. When inserted into the reagent introduction reactor 800, the reagent previously stored in the reagent introduction reactor 800 is sent to the flow path of the liquid supply pillar 262 through the liquid supply hole 263 provided at the top of the liquid supply pillar 262.

[0083] The reagent introduction reactor 800 can be pre-stored with a reagent (additive reagent) and mineral oil (extrusion reagent). When both the additive reagent and the extrusion reagent are pre-stored in the reagent introduction reactor, substances are selected such that the specific gravity of the reagent (additive reagent) is greater than the specific gravity of the mineral oil (extrusion reagent). This allows a layer of mineral oil to be formed on top of the liquid layer of the reagent (additive reagent) in the reagent introduction reactor 800. As described above, when the upper end of the liquid supply pillar 262 pushes up the lid 803 and is inserted into the reagent introduction reactor 800, the liquid supply hole 263 of the liquid supply pillar 262 is configured to be located in the liquid layer of the reagent (additive reagent). To achieve this configuration, the length of the liquid supply pillar 262, the position where the liquid supply hole 263 is formed in the liquid supply pillar 262, the volume of the reagent introduction reactor 800, etc. are adjusted in advance. This configuration realizes a mechanism in which the reagent (added reagent) first flows into the flow path of the liquid supply pillar 262 through the liquid supply hole 263, and after the reagent flows out of the reagent introduction reactor 800, mineral oil flows into the flow path of the liquid supply pillar 262 through the liquid supply hole 263. When supplying the liquid, a cap unit 40 (FIG. 7) is used as in the first embodiment. In this embodiment, the cap unit 40 is attached to the top of the reagent introduction reactor 800 and the reagent introduction holder 900, and air pressure is sent into the reagent introduction reactor 800 to press down the liquid level of the liquid stored in the reagent introduction reactor 800, and the liquid is sent through the liquid supply hole 263.

[0084] The reagent that first reaches the internal space 22 of the introduction main body 20 from the flow path of the liquid delivery pillar 262 is sucked into the capillary portion 10, and the excess reagent is absorbed into the textile product 70. This has been explained in the first embodiment, so the explanation here will be omitted.

[0085] In this embodiment, surfaces that come into contact with the reagent, such as the inner surface of the reagent introduction reactor 800 and the inner surface of the liquid delivery pillar 262, are also treated with a hydrophilic anti-adsorption coating or other surface treatment to prevent adsorption of nucleic acids and proteins in the sample or reagent.

[0086] The connection portion between the reagent introduction holder 900 and the inlet 26A will be further described. As shown in FIGS. 13 and 14, a convex portion 264 that protrudes upward is provided on the upper surface of the lower connector portion 261 of the inlet 26A. A concave portion 904 that fits with the convex portion 264 is provided at a location of the reagent introduction holder 900 facing the convex portion 264. A soft material made of highly adhesive silicone rubber or the like is mounted near the center of each part. As described above, by lowering the reagent introduction holder 900 toward the inlet 26A, the convex portion 264 and the concave portion 904 fit together. This connects the flow path of the reagent introduction holder 900 to the inlet 26A and the flow path of the inlet 26A. Furthermore, the soft materials tightly contact and seal the gaps in the connecting portions, preventing leakage of the reagent from the gaps in the connecting portions.

[0087] The fitting locations of the convex portion 264 and the concave portion 904 are provided, for example, in the outer peripheral region of the upper surface when the upper surface of the connector lower portion 261 is viewed from above. In one example, as shown in Fig. 14, the fitting locations are provided at positions symmetrical with respect to the liquid delivery pillar 262 located in the center. In Fig. 15, which will be described later, the convex portions 264 are provided in each of the four corner regions of the upper surface of the connector lower portion 261, which has a quadrangular shape when viewed from above.

[0088] [2] Test kit The test kit of this embodiment includes the dispenser system 1A of this embodiment. Figure 15 is an exploded view of the test kit 100A of this embodiment. For ease of explanation, the cap part 40 is not shown.

[0089] As in the first embodiment described above, the test kit 100A includes a holder 301 that supports at least the dispenser system 1A and the reactor chip 302, a holder stand 307 on which the holder 301 can be mounted, and a container 308 on which the holder stand 307 can be mounted. The container 308 is configured to be able to contain palmitic acid, for example. The test kit 100A uses molten palmitic acid sealed in a palmitic acid container and heats it. The palmitic acid in the container is maintained at its melting point of 62.9°C while it transitions from a liquid to a solid, thereby performing the LAMP method. This allows the LAMP method to be performed without using electrical control. The test kit 100A is generally equivalent to the test kit 100 of the first embodiment, and therefore a detailed description thereof will be omitted here.

[0090] As described above, according to this embodiment, a practical dispenser system that can be used to add a small amount of reagent for POCT can be realized, similarly to the first embodiment. Furthermore, according to this embodiment, it is possible to carry out an independent reaction in the container 800. For example, after a sample purification reaction is carried out in the container 800, the sample after the purification reaction is sent to the dispenser system 1A and introduced into the reactor chip 302 to carry out the LAMP method. Such a two-step reaction can be completed and carried out in the dispenser system by simply performing a simple operation of lowering the reagent introduction holder 900 toward the inlet 26A.

[0091] According to the configurations of the above-described embodiments, a practical dispenser system that can be used to add small amounts of reagents for POCT can be realized. Such effects also contribute to the achievement of, for example, Goal 3 of the Sustainable Development Goals (SDGs) advocated by the United Nations, "Ensure healthy lives and promote well-being for all at all ages."

[0092] The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope of the claims. Embodiments obtained by appropriately combining the technical means disclosed in different embodiments are also included in the technical scope of the present invention. [Example]

[0093] An embodiment of the present invention will now be described.

[0094] (Example: Detection of viral genes using LAMP method) A viral gene detection experiment using the LAMP method was carried out using a sample to which a reagent had been added using the dispenser system of the aspect described in the first embodiment above.

[0095] The dispenser system 1 used had an internal space 22 of the introduction body 20 with a base of 1 cm square, a depth of 2.5 mm, and an internal volume of 180 μL. Each loading section 27 was fitted with a 2 mm x 12 mm strip of Japanese paper (0.27 mm thick, manufactured by Daifuku Washi Kogyo) as the textile processed material 70. The inlet 26 and introduction body 20 were each 3D printed using standard black resin (manufactured by Okamura Tech Co., Ltd.), and the surfaces of the shaped parts that come into contact with the reagent were coated with a hydrophilic coating (Prevelex, manufactured by Nissan Chemical Industries) and a hydrophobic coating (Cytop, manufactured by AGC). The capillary section 10 was made of glass (inner diameter 0.2 mm, manufactured by AS ONE Corporation), and nine capillaries were installed, as in the embodiment.

[0096] Next, 50 μL of test sample (additive reagent) was prepared to be introduced into the inlet 26. The test sample was of the following three types: (Sample No. 1) Sample containing adenovirus DNA (Sample No. 2) Sample containing adenovirus type 41 DNA (Sample No. 3) Sample containing cytomegalovirus DNA. The preparation of each sample was as follows:

[0097] (Sample No. 1) Sample containing adenovirus DNA Adenovirus DNA (MBC001, Vircell) was added to saliva samples from healthy volunteers to a final concentration of 100 copies / μL, and then MagMAX® was added to a final concentration of 50 μg / μL. TM DNA and RNA degrading enzymes in the saliva were inactivated by adding Viral / Pathogen Proteinase K (Thermofisher).The sample was then heated at 95°C for 5 minutes to inactivate the Proteinase K.

[0098] (Sample No. 2) Sample containing adenovirus type 41 DNA Adenovirus type 41 DNA (MBC114, Vircell) was added to a saliva sample from a healthy subject to a final concentration of 100 copies / μL, and then MagMAX® was added to a final concentration of 50 μg / μL. TM DNA and RNA degrading enzymes in the saliva were inactivated by adding Viral / Pathogen Proteinase K (Thermofisher).The sample was then heated at 95°C for 5 minutes to inactivate the Proteinase K.

[0099] (Sample No. 3) Sample containing cytomegalovirus DNA Cytomegalovirus DNA (MBC016, Vircell) was added to saliva samples from healthy volunteers to a final concentration of 100 copies / μL, and then MagMAX® was added to a final concentration of 50 μg / μL. TM DNA and RNA degrading enzymes in the saliva were inactivated by adding Viral / Pathogen Proteinase K (Thermofisher).The sample was then heated at 95°C for 5 minutes to inactivate the Proteinase K.

[0100] The above-described dispenser system 1 was prepared for each specimen sample. As described in the embodiment, the specimen sample was extruded using mineral oil. After the specimen sample was introduced into the inlet 26, 50 μL of mineral oil was introduced into the inlet 26. Then, the cap portion 40 was attached to the inlet 26 and crushed, and air pressure was sent to the inlet 26. The cap portion 40 was made of rubber and had a cavity 41 with an internal volume of 200 μL.

[0101] Using the above procedure, 500 nL of specimen sample was dispensed into nine wells 302a of the reactor chip 302 located downstream of the test kit 100. Mineral oil was also added to the reactor chip 302, as shown in Figure 11. Using this reactor chip 302 and the test kit 100, the LAMP method was performed to detect viral genes. The detection results are shown in Figure 16.

[0102] In Figure 16, an explanation of each well 302a is provided at the top of the drawing. The bottom of the drawing shows imaging data obtained by imaging light obtained by testing three types of specimen samples using the test kit 100. The imaging data shows a sample containing adenovirus DNA (sample No. 1) at the left end, a sample containing adenovirus type 41 DNA (sample No. 2) in the middle, and a sample containing cytomegalovirus DNA (sample No. 3) at the right end. Each reactor chip 302 was provided with a positive control well 302a and a negative control well 302a, as shown at the top of Figure 16.

[0103] As shown in the captured image in Figure 16, in this example, an accurate amount of specimen sample was added to each well, and it was demonstrated that specific detection of the target viral gene was possible using the LAMP method.

[0104] The results of this example demonstrate that the use of dispenser system 1 makes it possible to provide a disposable dispenser system that can dispense multiple sub-microliter scale reagents simultaneously with simple operations without using electrical control.

[0105] By incorporating a dispenser system according to one embodiment of the present invention into a portable device for testing for viral infection or a biomimetic portable device for testing for drug contamination, it becomes possible to develop a practical POCT device.

[0106] 〔summary〕 [1] A dispenser system having an internal space with an open upper end of a capillary section capable of sucking in a certain amount of reagent or sample by capillary action, an introduction section capable of introducing the reagent or sample into the internal space from an upper inlet, and a pressurizing mechanism capable of pressurizing the internal space in the introduction section, wherein the reagent or sample sucked into the capillary section is added to a target location by pressurizing the internal space with the pressurizing mechanism, and a loading section loaded with a processed fiber material capable of absorbing excess reagent or excess sample remaining in the internal space when the reagent or sample has been sucked into the capillary section is provided outside the internal space in the introduction section.

[0107] [2] The dispenser system described in [1], wherein the loading section and the internal space are separated by a partition section having a communication hole that connects the loading section and the internal space in part, and the communication holes are multiple and arranged in positions symmetrical around the internal space.

[0108] [3] A dispenser system as described in [2], wherein the loading sections are arranged in a row to surround the internal space, each loading section is connected to the internal space via the communication hole, and the fiber processed product is loaded into each loading section as an independent structure.

[0109] [4] A dispenser system described in [2] or [3], wherein the internal space has a polygonal shape when viewed from above the introduction portion, the inlet is located in the central part of the polygonal internal space, and the communicating hole is located in a corner region of the polygonal internal space.

[0110] [5] A dispenser system described in any of [1] to [4], wherein the pressurizing mechanism is a cap portion arranged above the introduction portion, has a hollow portion, and the internal volume of the hollow portion changes when pressed from the outside of the cap portion, and is configured so that the reagent or sample sucked into the capillary portion is added to the target location by the pressing of the cap portion, and the cap portion is configured to be detachable from the introduction portion on the inlet side of the introduction portion.

[0111] [6] A dispenser system according to any one of [1] to [5], further comprising a support part for supporting the capillary portion.

[0112] [7] A dispenser system described in any of [1] to [6], wherein the capillary portion has an internal volume into which the reagent or sample is dispensed on the submicroliter scale, nanoliter scale, or microliter scale.

[0113] [8] A dispenser system according to any one of [1] to [7], wherein the processed fiber product is Japanese paper.

[0114] [9] A dispenser system described in any of [1] to [8], wherein when the reagent or sample is sucked into the capillary portion and excess reagent or excess sample remaining in the internal space is absorbed by the textile processed product, mineral oil is introduced into the internal space, and when the mineral oil is introduced, the internal space is pressurized by the pressure mechanism, thereby adding the reagent or sample sucked into the capillary portion to the target location.

[0115]

[10] A test kit comprising the dispenser system described in any one of [1] to [9] above, and a test device that can be equipped with the dispenser system and performs a test using the reagent or sample added from the dispenser system.

[0116]

[11] A method for adding a reagent using the dispenser system described in any one of [1] to [9] above, comprising: a preparation step of introducing the reagent or sample from the inlet into the internal space of the introduction part, and after the reagent or sample has been sucked into the capillary part, allowing the textile product to absorb any excess reagent or excess sample remaining in the internal space; and an addition step of adding the reagent or sample sucked into the capillary part to a target location by pressurizing the internal space using the pressure mechanism after the preparation step.

[0117]

[12] The reagent addition method described in

[11] , further comprising an oil introduction step between the preparation step and the addition step, of introducing mineral oil into the internal space from the inlet. [Industrial Applicability]

[0118] The present invention can provide a POCT dispenser that can add small amounts of reagents, and can therefore be used for early infection diagnosis and rapid isolation using portable devices for viral infection testing, and for rapid drug contamination testing at disaster sites and the like using biomimetic portable devices. [Explanation of symbols]

[0119] 1, 1A: dispenser system, 2: introduction part, 10: capillary part, 10a: upper end, 20: introduction main body part, 22: internal space, 26, 26A: inlet, 26a: entrance, 26b: lower opening, 27: loading part, 28: partition part, 28a: partial partition body, 30: support part, 40: cap part, 41: cavity part, 70: textile processed product, 80: communication hole, 100, 100A: inspection kit, 260a to 260h: recessed grooves, 262: Liquid delivery pillar, 300: Testing device, 301: Holder, 302: Reactor chip, 302a: Well, 303: Heating container, 307: Holder stand, 308: Container, 400: Imaging device, 800: Reagent introduction reactor, 801: Bottom surface, 900: Reagent introduction holder, 902: Opening, 904: Recess, S1: Reagent addition method, S11: Preparation step, S21: Oil introduction step, S31: Addition step

Claims

1. an introduction part having an internal space with an open upper end of a capillary part capable of sucking in a fixed amount of reagent or sample by capillary action, and capable of introducing the reagent or sample into the internal space from an upper inlet; a pressurizing mechanism capable of pressurizing the internal space in the introduction portion; A dispenser system comprising: The internal space is pressurized by the pressurizing mechanism, whereby the reagent or sample sucked into the capillary portion is added to a target location. a loading section loaded with a processed fiber material capable of absorbing excess reagent or excess sample remaining in the internal space when the reagent or sample is sucked into the capillary section is provided outside the internal space of the introduction section; Dispenser system.

2. the loading section and the internal space are separated by a partition wall having a communication hole that communicates a part of the loading section with the internal space, The communication holes are provided in plural and are arranged symmetrically with respect to the internal space. The dispenser system of claim 1 .

3. A plurality of the loading sections are arranged side by side so as to surround the internal space, Each of the loading sections communicates with the internal space via the communication hole, The fiber processed product is loaded in each of the loading sections as an independent configuration. The dispenser system of claim 2 .

4. the internal space has a polygonal shape when viewed from above the introduction portion, the inlet is located in a central portion of the polygonal interior space; The communication holes are located in corner regions of the polygonal internal space.

4. The dispenser system according to claim 2 or 3.

5. the pressurizing mechanism is a cap portion disposed above the introduction portion, the cap portion having a cavity portion, and an internal volume of the cavity portion changing when the cap portion is pressed from outside, The cap portion is pressed to add the reagent or sample sucked into the capillary portion to a target location, The cap portion is configured to be detachable from the introduction portion on the inlet side of the introduction portion.

3. A dispenser system according to claim 1 or 2.

6. Further provided is a support part that supports the capillary portion.

3. A dispenser system according to claim 1 or 2.

7. The capillary portion has an internal volume into which the reagent or sample is dispensed, the internal volume being on a submicroliter scale, a nanoliter scale, or a microliter scale.

3. A dispenser system according to claim 1 or 2.

8. The processed fiber product is Japanese paper.

3. A dispenser system according to claim 1 or 2.

9. a configuration in which mineral oil is introduced into the internal space in a state in which the reagent or sample is sucked into the capillary portion and excess reagent or excess sample remaining in the internal space is absorbed by the fiber processed product; In a state where the mineral oil is introduced, the internal space is pressurized by the pressurizing mechanism, whereby the reagent or sample sucked into the capillary portion is added to a target location.

3. A dispenser system according to claim 1 or 2.

10. The dispenser system of claim 1; a testing device that can be equipped with the dispenser system and that performs a test using a reagent or a sample added from the dispenser system; Equipped with Test kit.

11. A method for adding a reagent using the dispenser system according to claim 1, comprising: a preparation step of introducing the reagent or sample into the internal space of the introduction part from the inlet, and after the reagent or sample is aspirated into the capillary part, allowing excess reagent or excess sample remaining in the internal space to be absorbed by the textile product; an adding step of adding the reagent or sample sucked into the capillary portion to a target location by pressurizing the internal space with the pressurizing mechanism after the preparing step; Including, Reagent addition method.

12. an oil introducing step of introducing mineral oil into the internal space from the inlet between the preparing step and the adding step; The method for adding a reagent according to claim 11.

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