Method, device, and storage medium for sorting magnetic beads

Abstract

This application discloses a method, device, and storage medium for sorting magnetic beads. The method includes placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, adsorbing the immunomagnetic bead cells in the liquid within the liquid bag by the magnetic platform, controlling the oscillation of the magnetic platform according to preset oscillation parameters to uniformly distribute the immunomagnetic bead cells adsorbed on the magnetic platform and suspend other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid, performing a waste liquid discharge operation to allow the liquid in the liquid bag other than the adsorbed immunomagnetic bead cells to flow into a waste liquid bag, washing the immunomagnetic bead cells remaining in the liquid bag, injecting a resuspension liquid into the liquid bag, and after taking out the liquid bag containing the immunomagnetic bead cells, confirming that the sorting of the magnetic beads is completed. In this application, the immunomagnetic bead cells adsorbed on the magnetic platform are uniformly distributed without local accumulation, improving the utilization rate of the magnetic field and realizing the efficient and stable separation and recovery of the immunomagnetic bead cells.

Description

Technical Field

[0001] This application claims the priority of a Chinese patent application with an application number of 202211182777.5 and an invention title of "Method, Device, and Storage Medium for Separating Magnetic Beads", which was filed with the Chinese Patent Office on September 27, 2022. The entire content of the said application is incorporated herein by reference. This application relates to the technical field of magnetic bead separation, and particularly to a method, device, and storage medium for separating magnetic beads.

Background Art

[0002] Currently, in magnetic bead separation technology, immunomagnetic bead cells (some cell surface antigens have the property of binding to specific antibodies connected to magnetic beads. Such cells are connected to magnetic beads through antigens in an external magnetic field, thereby forming immunomagnetic bead cells.) bound to magnetic beads are adsorbed by a magnet, and other substances that are not adsorbed by the magnet (such as cells that cannot bind to magnetic beads) are separated to separate the immunomagnetic bead cells. The inventors have noticed that in the prior art, magnetic bead separation is usually performed by combining a microfluidic chip and a magnetic field, but this solution has the problem of small processing capacity. Thus, when processing a large amount of cell fluid, since the area of the liquid bag is too large, the flow rate of the liquid passing through each region when flowing in the liquid bag is not constant, and as a result, the magnetization of the region corresponding to each cross-sectional area of the fluid bag becomes non-uniform. For example, if the flow rate in some regions is too fast, the contact between the immunomagnetic bead cells and the magnetic field is insufficient, so the immunomagnetic bead cells escape. As a result, the utilization rate of the magnet is low, the adsorption of immunomagnetic bead cells by the magnet is not sufficient, and the adsorbed immunomagnetic bead cells accumulate in large quantities near the regions with a low flow rate, which also causes insufficient adsorption of the magnet.

Summary of the Invention

Problems to be Solved by the Invention

[0003] Embodiments of the present application provide a method, device, and storage medium for sorting magnetic beads to solve problems such as low utilization rate of magnetic fields in the prior art.

Means for Solving the Problems

[0004] A method for sorting magnetic beads, comprising: Placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform; Controlling the oscillation of the magnetic platform according to preset oscillation parameters to uniformly distribute the immunomagnetic bead cells adsorbed on the magnetic platform, and suspending other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid; Performing a waste liquid discharge operation to allow the liquid other than the adsorbed immunomagnetic bead cells in the liquid bag to flow into a waste liquid bag; Washing the immunomagnetic bead cells remaining in the liquid bag; Injecting a resuspension into the liquid bag, taking out the liquid bag containing immunomagnetic bead cells, and confirming that the sorting of magnetic beads is completed.

[0005] A controller used to execute the above method for sorting magnetic beads.

[0006] A magnetic bead sorting device comprising a pressing device, a mixing device, and the above controller, wherein the controller is connected to the pressing device and the mixing device.

[0007] A computer-readable storage medium storing computer-readable instructions that, when executed by a processor, implement the above method for sorting magnetic beads.

Advantages of the Invention

[0008] In the above method, device and storage medium for sorting magnetic beads, the method includes the steps of placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform, controlling the oscillation of the magnetic platform according to preset oscillation parameters to uniformly distribute the immunomagnetic bead cells adsorbed on the magnetic platform and suspending other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid, performing a waste liquid discharge operation to allow the liquid other than the adsorbed immunomagnetic bead cells in the liquid bag to flow into a waste liquid bag, washing the immunomagnetic bead cells remaining in the liquid bag, injecting a resuspension into the liquid bag, taking out the liquid bag containing the immunomagnetic bead cells, and then confirming that the sorting of the magnetic beads is completed.

[0009] In the present application, by controlling the oscillation of the magnetic platform according to preset oscillation parameters, the liquid (e.g., cell fluid) in the large-capacity or high-throughput liquid bag is uniformly shaken, so that the immunomagnetic bead cells are uniformly distributed on the bottom surface of the liquid bag without local accumulation. Thereby, the magnetic platform adheres to the non-accumulated immunomagnetic bead cells in the uniformly shaken liquid bag, realizing sufficient adsorption of the immunomagnetic bead cells by the magnetic platform and avoiding loss of the immunomagnetic bead cells. Also, other substances in the liquid bag other than the adsorbed immunomagnetic bead cells can be suspended in the liquid (e.g., after shaking until other substances attached to the adsorbed immunomagnetic bead cells are separated from the immunomagnetic bead cells and then suspended in the liquid, such other substances are discharged when performing the waste liquid discharge operation without inhibiting the adsorption of the non-adsorbed immunomagnetic bead cells to the magnetic platform). Finally, through the waste liquid discharge operation and the washing operation, other substances other than the immunomagnetic bead cells are separated from the liquid bag, and finally the immunomagnetic bead cells are recovered. In the present application, the utilization rate of the magnetic field is improved, and efficient and stable separation and recovery of immunomagnetic bead cells by sorting the magnetic beads can be realized.

[0010] The details of one or more embodiments of the present application are described in the following drawings and description, and other features and advantages of the present application will become apparent from the specification, drawings, and claims.

Brief Description of the Drawings

[0011] To more clearly illustrate the technical solution of the embodiments of the present application, the drawings necessary for the description of the embodiments of the present application are briefly described below. However, the drawings in the following description are only some embodiments of the present application, and it is obvious that those skilled in the art can obtain other drawings based on these drawings without creative effort.

Figure 1

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Embodiments for Carrying Out the Invention

[0012] Hereinafter, with reference to the drawings in the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. It is obvious that the described embodiments are only a part of the embodiments of the present application, not all of them. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the present application.

[0013] In one embodiment, as shown in FIG. 1, a method for sorting magnetic beads including the following steps S10 to S50 is provided. S10: Place the empty liquid bag 50 on the magnetic platform 10, inject the liquid containing immunomagnetic bead cells into the liquid bag 50, and adsorb the immunomagnetic bead cells in the liquid in the liquid bag 50 by the magnetic platform 10. Here, the magnetic platform 10 may be a magnetic platform composed of an electromagnet or a permanent magnet. For example, the magnetic platform 10 shown in FIG. 5 may include a base 101 provided with a lift through hole 1011, and a permanent magnet magnetic block 102 or an electromagnet attached to the base 101. The base 101 is provided with a mounting groove, and the magnetic block 102 is fixedly attached to this mounting groove. As can be understood, when the magnetic platform 10 is in close contact with the contact surface of the liquid bag 50, the liquid can be brought into close contact with the magnetic field of the magnetic platform 10 at a short distance, and the adsorption of immunomagnetic bead cells to the contact surface can be avoided. On the other hand, the liquid bag 50 is made of a non-magnetic material and is not magnetically adsorbed, so it is not affected by the magnetic field of the magnetic platform 10 below it.

[0014] In this step of the present application, place the empty liquid bag 50 on the magnetic platform 10 (for example, the magnetic block 102), and let the cell fluid in the sample container (the cell fluid refers to the mixed solution obtained by incubating cells, magnetic beads, and antibodies) flow into the empty liquid bag 50. That is, the sample container contains the cell fluid obtained by incubating cells, magnetic beads, and antibodies. The cell fluid contains immunomagnetic bead cells, and the immunomagnetic bead cells are the objects to be recovered by the magnetic bead sorting method of the present application. All other substances in the cell fluid other than the immunomagnetic bead cells need to be discharged into the waste liquid bag 50.

[0015] S20: Control the swinging of the magnetic platform 10 according to preset swinging parameters, evenly distribute the immunomagnetic bead cells adsorbed on the magnetic platform 10, and suspend other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells in the liquid. As can be understood, when processing a large volume of cell fluid, since the area of the liquid bag 50 is too large, the flow rate of the liquid passing through each region when flowing in the liquid bag 50 is not constant, and as a result, the magnetization of each region of the liquid bag 50 becomes non-uniform. Therefore, in the region with a low flow rate (near the region with a low flow rate), a large number of adsorbed immunomagnetic bead cells accumulate, and furthermore, some of the other substances other than the adsorbed immunomagnetic bead cells will adhere to the adsorbed immunomagnetic bead cells. In this way, in the region where immunomagnetic bead cells (and other substances attached thereto) accumulate (since a large number of other immunomagnetic bead cells and attached other substances accumulate between the magnetic platform 10 and the unadsorbed immunomagnetic bead cells in the liquid, the adsorption distance becomes large and the magnetic adsorption force may decrease.), it becomes difficult for the unadsorbed immunomagnetic bead cells in the liquid to be adsorbed. This causes loss of immunomagnetic bead cells and a decrease in the utilization rate of the magnetic field. In this embodiment, in order to avoid the loss of immunomagnetic bead cells due to not being adsorbed on the magnetic platform 10, it is necessary to prevent the immunomagnetic bead cells and other substances attached thereto from accumulating in one place. Also, the magnetic force of the magnetic platform 10 should not be too strong (if the magnetic force is too strong, the cells will die, so the above problem of loss of immunomagnetic bead cells cannot be solved even by increasing the magnetic force.). Therefore, it is necessary to appropriately set the preset swinging parameters, swing slowly, evenly oscillate the liquid in the liquid bag 50, so that the accumulated immunomagnetic bead cells are evenly distributed without further accumulation, and suspend other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells in the liquid instead of attaching them (it can be easily discharged when performing the waste liquid discharge operation, and the magnetic platform does not prevent the adsorption of unadsorbed immunomagnetic bead cells.).Excessive oscillation is also avoided (if the oscillation is too intense, since the magnetic force is weak, a large number of adsorbed immunomagnetic bead cells will separate from the magnetic platform 10, and as a result, some of the immunomagnetic bead cells will not be re-adsorbed and will be lost. In severe cases, because the force of the oscillation is too great, there is even a risk that the immunomagnetic bead cells will die.). Therefore, in this application, it is necessary to precisely control all of the preset oscillation parameters such as the angular velocity and angle for uniform oscillation. Specifically, first, it is necessary to obtain the preset oscillation parameters, and the preset oscillation parameters may be the oscillation angular velocity, oscillation angle, oscillation time, angular acceleration, and stop angle after the oscillation of the magnetic platform 10, etc. Specifically, the range of the oscillation angle of the magnetic platform 10 may be any within ±35 degrees (that is, the magnetic platform 106 oscillates towards both sides, and the angle of oscillation towards one side is in the range of 0 to 35 degrees. If this angle exceeds 35 degrees, there is a risk that the immunomagnetic bead cells will die.), preferably ±25 degrees.

[0016] S30: Execute a waste liquid discharge operation to allow the liquid in the liquid bag 50 other than the adsorbed immunomagnetic bead cells to flow into the waste liquid bag 50. In this step, in order to separate and collect the immunomagnetic bead cells smoothly, by opening the waste liquid discharge pipe, other substances in the liquid bag 50 other than the immunomagnetic bead cells can be discharged into the waste liquid bag 50 through the waste liquid discharge pipe.

[0017] S40: Wash the immunomagnetic bead cells remaining in the liquid bag 50. In this step, considering that other substances may be attached to the immunomagnetic bead cells remaining in the liquid bag 50, in order to increase the purity of the recovered immunomagnetic bead cells, the washing pipe is opened to wash the attached other substances, so that only the immunomagnetic bead cells are adsorbed to the magnetic platform 10 and thus retained in the liquid bag 50.

[0018] S50: After injecting the resuspension into the liquid bag 50 and taking out the liquid bag 50 containing immunomagnetic bead cells, it is confirmed that the selection of magnetic beads is completed. That is, injecting the resuspension into the liquid bag 50 is for suspending the immunomagnetic bead cells so as not to die until culturing. As can be understood, the above resuspension may be changed to a medium such as a liquid that can maintain the activity of immunomagnetic bead cells.

[0019] In the present application, the magnetic platform 10 may be oscillated and controlled according to preset oscillation parameters, whereby the liquid (for example, cell fluid) in the large-capacity or high-throughput liquid bag 50 is uniformly shaken, and the immunomagnetic bead cells are uniformly distributed on the bottom surface of the liquid bag 50 without accumulating in a local location (for example, near a region with a low flow rate). Thereby, the magnetic platform 10 adheres to the non-accumulated immunomagnetic bead cells in the uniformly shaken liquid bag 50, realizes sufficient adsorption of the immunomagnetic bead cells by the magnetic platform 10, and avoids loss of the immunomagnetic bead cells. Also, other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells can be suspended in the liquid (for example, after shaking until other substances attached to the adsorbed immunomagnetic bead cells are separated from the immunomagnetic bead cells and then suspended in the liquid, when performing the waste liquid discharge operation without inhibiting the adsorption of the non-adsorbed immunomagnetic bead cells to the magnetic platform 10, such other substances are discharged). Finally, through the waste liquid discharge operation and the washing operation, other substances other than the immunomagnetic bead cells are separated from the liquid bag 50, and finally the immunomagnetic bead cells are recovered. In the present application, the utilization rate of the magnetic field is improved, and efficient and stable separation and recovery of immunomagnetic bead cells by the selection of magnetic beads can be realized. In the present application, a large amount of immunomagnetic bead cells can be directly selected by magnetic beads, realizing the selection of a large amount of magnetic beads in a short time without impairing the cell viability. Also, since this process does not require manual operation, the selection efficiency of a large amount of magnetic beads is improved.

[0020] In one embodiment, the step S10 of placing the empty liquid bag 50 on the magnetic platform 10, injecting a liquid containing immunomagnetic bead cells into the liquid bag 50, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag 50 by the magnetic platform 10 includes the following steps. Place the empty liquid bag 50 on the magnetic platform 10 of the pressing device 1. As shown in FIG. 5, the pressing device 1 includes a closing mechanism 30 attached to the magnetic platform 10 and a pressing mechanism 40 connected to the closing mechanism 30. After the closing mechanism 30 is closed, the liquid bag 50 is located in the accommodation space between the closing mechanism 30 and the magnetic platform 10. The closing mechanism 30 is attached to the magnetic platform 10 and can open and close the above-mentioned accommodation space (when the liquid bag 50 placed in the accommodation space bulges and the protruding height becomes high, the closing mechanism 30 may be pushed up by the bulging liquid bag 50 and cannot be closed. If forced to close, the liquid bag 50 may burst or the immunomagnetic bead cells or anucleated immune cells therein may be damaged.). Also, when there is no interference by other external forces, the closing mechanism 30 can move downward by being pulled downward by the pressing mechanism 40, whereby the size of the accommodation space between the platform 10 for placing the liquid bag 50 and the closing mechanism 30 can be adjusted.

[0021] Inject a liquid containing immunomagnetic bead cells into the liquid bag 50. In the above process, a flow control assembly (not shown) may be provided between the liquid bag 50 and the sample container. After the flow control assembly is activated, the liquid containing immunomagnetic bead cells can also be automatically injected into the liquid bag 50. Also, the liquid containing immunomagnetic bead cells may be injected into the liquid bag 50 by placing the sample container at a high position and pressing the sample container. Here, the flow control assembly may include a peristaltic pump that can provide power for the liquid to flow, and a pinch valve for opening and closing the flow control assembly, and may also include a flow adjustment valve for controlling the flow rate of the liquid, a sensor for monitoring the flow rate and pressure of the liquid, and the like.

[0022] After the closing mechanism 30 is closed, the pressing mechanism 40 is controlled to move the closed closing mechanism 30 downward, flattening the liquid bag 50 placed in the accommodation space between the magnetic platform 10 and the closing mechanism 30, increasing the contact surface between the bottom surface of the liquid bag 50 and the magnetic platform 10, and further adsorbing the immunomagnetic bead cells in the liquid bag 50 to the contact surface by the magnetic platform 10. As can be understood, after the closing mechanism 30 is closed, the pressing mechanism 40 moves the closed closing mechanism 30 downward to press the liquid bag 50 uniformly and flatly, increasing the contact surface obtained by the close contact between the liquid bag 50 and the magnetic platform 10. Further, the liquid in the liquid bag 50 is uniformly distributed on the magnetic platform 10 through this contact surface and can contact the magnetic platform. In this embodiment, by pressing the liquid bag 50 flatly, the contact surface between the bottom surface of the liquid bag 50 and the magnetic platform 10 is increased. Further, the magnetic platform 10 can more sufficiently adsorb the immunomagnetic bead cells in the liquid bag 50 with the increased contact surface, thereby avoiding the escape of the immunomagnetic bead cells, further improving the utilization rate of the magnetic field, and realizing the efficient and stable capture of the immunomagnetic bead cells while ensuring the simplicity of the operation.

[0023] In one embodiment, the pressing device 1 further includes a lift mechanism 20 connected to the closing mechanism 30. Further, in step S10, after injecting the liquid containing the immunomagnetic bead cells into the liquid bag 50, the following steps are further included. When the closing mechanism 30 cannot be closed because it is pushed up by the inflated liquid bag 50, the method further includes the step of increasing the accommodation space between the magnetic platform 10 for arranging the liquid bag 50 and the closing mechanism 30 by controlling the lift mechanism 20 to lift the closing mechanism 30. In this embodiment, when the liquid bag 50 is arranged in the accommodation space, the height of the liquid in the liquid bag 50 swells to a high level. Therefore, when the closing mechanism 30 cannot be closed because it is pushed up by the liquid bag 50 placed in the accommodation space, the lift mechanism 20 is controlled to be activated to lift the closing mechanism 30. In this way, the distance between the magnetic platform 10 and the closing mechanism 30 increases, and the accommodation space also increases with the increase of the distance.

[0024] When the closing mechanism 30 rises to a preset height at which it can be closed, the closing mechanism 30 may be controlled to be closed. That is, when the closing mechanism 30 rises to the preset height, the accommodation space between the closing mechanism 30 and the magnetic platform 10 becomes sufficiently large, and the closing mechanism 30 will not be further pushed up by the liquid bag 50 and can be normally closed.

[0025] By controlling the pressing mechanism 40 to move the closed closing mechanism 30 downward, the liquid bag 50 placed in the accommodation space is pressed flat, the contact surface between the bottom surface of the liquid bag 50 and the magnetic platform 10 is increased, and further, the immunomagnetic bead cells in the liquid bag 50 are adsorbed on the contact surface by the magnetic platform 10. That is, after the closing mechanism 30 is closed, the pressing mechanism 40 can move the closed closing mechanism 30 downward to press the liquid bag 50 uniformly and flatly, thereby increasing the contact surface formed by the close contact between the liquid bag 50 and the magnetic platform 10. The liquid in the liquid bag 50 can be uniformly distributed through this contact surface and contact the magnetic platform.

[0026] In one embodiment, as shown in FIGS. 5 and 6, the magnetic platform 10 is provided with a lift through-hole 1011. The lift mechanism 20 includes a lifting assembly 201 provided on the upper part of the magnetic platform 10, a lift rod 202 whose upper end penetrates through the lift through-hole 1011 and is fixedly connected to the lifting assembly 201, and a lifting drive assembly 203 provided below the magnetic platform 10 and connected to the end of the lift rod 202 away from the lifting assembly 201. The closing mechanism 30 includes a cover 301 rotatably connected to the lifting assembly 201, and the shape of the cover 301 may be set according to the specific shape of the magnetic platform 10. The shape of the lifting assembly 201 can be set as needed. For example, the lifting assembly 201 may be a frame structure arranged on the upper part of the magnetic platform 10 (or may be composed of a plurality of individually provided assemblies, but it can be driven by the lift rod 202 to move up and down), and it may be driven by the lifting drive assembly 203 to move up and down. In one embodiment, the lifting assembly 201 includes a first lifting block 2011 provided at the first end of the magnetic platform 10, two second lifting blocks 2012 spaced apart and distributed at the second end of the magnetic platform 10 away from the first end, a limiting plate 2013 connected between the first lifting block 2011 and the second lifting blocks 2012, and a rotating shaft 2014 connected between the two second lifting blocks 2012. The cover 301 is rotatably connected to the rotating shaft 2014. Specifically, the limiting plate 2013 is two plates provided in parallel on both opposite sides of the magnetic platform 10. In this way, a limiting frame surrounded by the first lifting block 2011, the two second lifting blocks 2012, and the two limiting plates 2013 is obtained, which is used to prevent the liquid bag 50 from slipping out from the side of the accommodating space. This limiting frame (i.e., the main component of the lifting assembly 201) can move up and down in the vertical direction synchronously with the cover 301.

[0027] Furthermore, the step of increasing the accommodation space between the magnetic platform 10 for arranging the liquid bag 50 and the closing mechanism 30 by controlling the lift mechanism 20 to lift the closing mechanism 30 includes controlling and starting the lift drive assembly 203, and driving the lift rod 202 by the lift drive assembly 203 to lift the lifting and lowering assembly 201 and the cover 301 while sliding along the lift through hole 1011, so as to increase the accommodation space between the magnetic platform 10 and the cover 301 for arranging the liquid bag 50. That is, the closing mechanism 30 is rotatably attached to the lifting and lowering assembly 201 and moves as the lifting and lowering assembly 201 moves up and down. On the other hand, the lift drive assembly 203 can move the lifting and lowering assembly 201 upward along the lift through hole 1011 via the lift rod 202. In this way, the cover 301 is moved upward, and further, the accommodation space between the magnetic platform 10 and the cover 301 for arranging the liquid bag 50 is increased, facilitating the closing of the cover 301.

[0028] Furthermore, as shown in FIGS. 5 and 6, the closing mechanism 30 further includes a first adsorption portion 302 provided on the cover 301 and a second adsorption portion 303 provided at a position on the lifting assembly 201 that faces the first adsorption portion 302. The cover 301 is closed by the lifting assembly 201 through adsorption by the first adsorption portion 302 and the second adsorption portion 303. Here, at least one of the first adsorption portion 302 and the second adsorption portion 303 is a magnet. For example, both may be magnets, or one may be a magnet while the other may be a metal having the property of being adsorbed by a magnet. The combination of the numbers, specific shapes, and sizes of the above-mentioned first adsorption portion 302 and second adsorption portion 303 can be set as required and is not limited here. Furthermore, in the present application, the magnet may be an electromagnet. In one specific embodiment, the second adsorption portion 303 is an electromagnet and the first adsorption portion 302 is an iron block. In this case, when the electromagnet that is the second adsorption portion 303 is energized, the magnetism of the electromagnet can be removed. At this time, since the electromagnet of the second adsorption portion 303 and the first adsorption portion 302 (iron block) in the cover 301 do not have a magnetic adsorption function, they are in a separated state. Therefore, the cover 301 can be opened. On the other hand, when the power supply to the second adsorption portion 303 stops, magnetism is generated in the electromagnet that is the second adsorption portion 303. At this time, if there is no external force blocking, if the iron block in the cover 301 is within the magnetic attraction range of the electromagnet, the first adsorption portion 302 of the cover 301 is adsorbed by the second adsorption portion 303 and the cover 301 is closed.

[0029] In one embodiment, as shown in FIGS. 5 and 6, the lifting drive assembly 203 includes a second motor 2031, a second drive wheel 2032, a second driven wheel 2033, a second timing belt 2034, a second rotating shaft 2035, a cam 2036, a mounting bearing 2037, and a cam carrier 2038 having a second mounting hole 2039. The cam carrier 2038 is attached to the bottom of the magnetic platform 10. The second rotating shaft 2035 is mounted in the second mounting hole 2039 via the mounting bearing 2037. The cam 2036 is fixedly attached to the second rotating shaft 2035. The second drive wheel 2032 is attached to the output shaft of the second motor 2031. The second timing belt 2034 is socket-fixed to the second drive wheel 2032 and the second driven wheel 2033. The second driven wheel 2033 is fixedly attached to the second rotating shaft 2035. The upper end of the cam 2036 abuts against the lift rod 202. As shown in FIG. 5, the second motor 2031 may be fixed to the magnetic platform 10 by a fixing block. The cam 2036, the cam carrier 2038, and the mounting bearing 2037 of the second rotating shaft 2035 are all provided in two symmetrically. The lift rods 202 are also provided in two parallelly. The upper ends of the two lift rods 202 are all connected to the lifting assembly 201. On the other hand, the two cams 2036 respectively abut against the lower ends of the two lift rods 202.

[0030] Furthermore, the step of driving the lift rod 202 by the ascending drive assembly 203 to raise the lifting assembly 201 and the cover 301 while sliding them along the lift through-hole 1011 includes controlling and starting the second motor 2031, and driving the cam 2036 to rotate in sequence by the second motor 2031 through the second drive wheel 2032, the second timing belt 2034, the second driven wheel 2033, and the second rotating shaft 2035, and raising the lifting assembly 201 and the cover 301 while sliding them along the lift through-hole 1011 through the lift rod 202 by the rotating cam 2036. That is, when the second motor 2031 starts and rotates, it rotates the second drive wheel 2032, and further rotates the second driven wheel 2033 through the second timing belt 2034. On the other hand, when the second driven wheel 2033 rotates, the second rotating shaft 2035 and the two cams 2036 attached to the second rotating shaft 2035 rotate. At this time, the lift rod 202 in contact with the cam 2036 moves upward in conjunction with the rotation of the cam 2036, and further lifts the lifting assembly 201 and the cover 301 as a whole. At this time, as described in the above embodiment, when the second adsorption portion 303 is an electromagnet, the energization of the electromagnet which is the second adsorption portion 303 is stopped. By doing so, in the ascending process of the lifting assembly 201, the cover 301 also gradually ascends in conjunction with its ascending and becomes flush with the lifting assembly 201. When the iron block of the first adsorption portion 302 is adsorbed by the electromagnet of the second adsorption portion 303, it is confirmed that the cover 301 is closed.

[0031] Furthermore, as shown in FIG. 5, the ascending drive assembly 203 further includes a sensing block 2042 attached to the second rotating shaft 2035 and a photoelectric sensor 2043 attached to the magnetic platform 10. When the photoelectric sensor 2043 senses that the second rotating shaft 2035 drives the sensing block 2042 to rotate to a position facing the photoelectric sensor 2043, it determines that the cover 301 has risen to the preset height. Here, the preset height refers to the highest height that the lift rod 202 can drive the lifting assembly 201 and the cover 301 to rise, and this preset height can be set according to the specific size of the liquid bag 50. Specifically, the size and shape of the cam 2036 can be changed as needed.

[0032] In one embodiment, as shown in FIG. 5, the ascending drive assembly 203 further includes a bearing follower 2040 and a fixing plate 2041. The bearing follower 2040 is attached to the lower end of the lift rod 202 by the fixing plate 2041, and the cam 2036 is in contact with the bearing follower 2040. That is, the bearing follower 2040 can transmit the lift force generated by the rotation of the cam 2036 to the lift rod 202 well, making the ascent of the lift rod 202 more stable and controllable.

[0033] In one embodiment, as shown in FIGS. 5 and 6, the magnetic platform 10 is provided with a guide through hole 1012. The pressing mechanism 40 includes a guide shaft 401, a spring 402, and a linear bearing 403 mounted in the guide through hole 1012. A stopper 4011 is provided at the lower end of the guide shaft 401. The upper end of the guide shaft 401 passes through the linear bearing 403 and is connected to the lifting assembly 201. The spring 402 is fitted onto the guide shaft 401, and both ends of the spring 402 are respectively in contact with the linear bearing 403 and the stopper 4011. Further, the step of controlling the pressing mechanism 40 to move the closed closing mechanism 30 downward includes controlling the upward driving assembly 203 to stop, so that the biasing force of the spring 402 moves the guide shaft 401 downward along the guide through hole 1012, and further includes the step of moving the lifting assembly 201 and the cover 301 downward. That is, in this embodiment, the upward driving assembly 203 having the cam 2036 can only drive the lift rod 202 to move upward, but does not have a downward pulling force to pull the lifting assembly 201 downward. Therefore, the above pressing mechanism 40 is provided, the spring 402 is in a compressed state, and after the second motor 2031 of the upward driving assembly 203 stops (after the second motor 2031 is reset), the spring 402 resists its compressed state and applies a downward pulling force to the lifting assembly 201 through the guide shaft 401 (the spring 402 drives the guide shaft 401 to move downward along the guide through hole 1012), and further drives the lifting assembly 201 and the cover 301 to move downward. As can be understood, if one guide shaft 401, one spring 402, and one linear bearing 403 are taken as a set of pressing assemblies, the above pressing mechanism 40 may include a plurality of pressing assemblies provided on the magnetic platform 10, for example, four sets of pressing assemblies provided at the four corners of the rectangular magnetic platform 10.In this way, due to the downward pulling force applied by the four springs 402 of the four pressing assemblies on the lifting assembly 201 simultaneously, when the lifting assembly 201 moves downward, the cover 301 presses the liquid bag 50 towards the magnetic platform 10 until it becomes flat, and distributes the liquid therein evenly on the magnetic platform 10. In the present application, instead of the second motor 2031 or the like, the spring 402 is pulled downward to press the liquid bag 50 flat. On the one hand, the spring 402 can press the liquid bag 50 evenly and flatly to make it adhere to the magnetic platform 10. On the other hand, since the biasing force of the spring 402 is uniform and small, the cells in the liquid bag 50 will not be crushed by being pressed.

[0034] In one embodiment, as shown in FIG. 2, the step S20 of controlling the oscillation of the magnetic platform 10 according to the preset oscillation parameters includes the following steps S201 and S202. S201: Obtain preset mixing parameters including a mixing angle and a mixing time. Here, the shown mixing angle is ±35 degrees (that is, the magnetic platform 106 oscillates on both opposite sides, and the angle of oscillation on one side is within the range of 0 to 35 degrees. If it exceeds 35 degrees, the immunomagnetic bead cells will die, so the maximum absolute value of the mixing angle is 35 degrees.), preferably ±25 degrees, and the mixing time length may be 25 seconds to 35 seconds, but preferably 30 seconds.

[0035] S202: Controlling the magnetic platform 10 by the mixing device 2 so as to swing continuously for the mixing time at the mixing angle, uniformly adsorbing immunomagnetic bead cells onto the magnetic platform 10, and suspending other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells in the liquid in conjunction with the swing of the magnetic platform 10. As can be understood, in one specific embodiment, as shown in FIGS. 3, 4, and 5, the mixing device 2 includes a first motor 21, a speed reducer 22, a first driving wheel 23, a first driven wheel 24, a first timing belt 25, and a synchro bracket 27. The speed reducer 22 is connected to the output shaft of the first motor 21 to reduce the rotation speed of the first motor 21 and increase the output torque. The first driving wheel 23 is connected to the rotating shaft 2014 of the speed reducer 22. The first timing belt 25 is socket-fixed to the first driving wheel 23 and the first driven wheel 24. The first driven wheel 24 is attached to the magnetic platform 10. The magnetic platform 10 is rotatably attached to the synchro bracket 27. The first driving wheel 23 is driven by the speed reducer 22 to rotationally drive the first driven wheel 24 via the first timing belt 25, and further swing the magnetic platform 10 around the fulcrum of the synchro bracket 27 to mix the liquid in the liquid bag 50 loaded thereon. Specifically, the first motor 21 may be a stepping motor. In the present application, since the torque output from the first motor 21 may be too small to oscillate the magnetic platform 10, the speed reducer 22 is used to reduce the rotation speed of the first motor 21 and increase the output torque. Finally, the magnetic platform 10 is swung at a predetermined angle by the interlocking of the first driving wheel 23, the first timing belt 25, and the first driven wheel 24. The liquid in the liquid bag 50 loaded on the magnetic platform 10 is mixed by the swing of the magnetic platform 10, the immunomagnetic bead cells are uniformly adsorbed onto the magnetic platform 10, and other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells are suspended in the liquid in conjunction with the swing of the magnetic platform 10.

[0036] In one embodiment, as shown in FIGS. 3 to 5, the mixing device 2 further includes a bearing 26, a first mounting hole 271 is provided in the synchro bracket 27, the magnetic platform 10 includes a first rotating shaft 103, and the first rotating shaft 103 is rotatably mounted in the first mounting hole 271 by the bearing 26. The first driven wheel 24 is mounted on the first rotating shaft 103. That is, when the first motor 21 drives the magnetic platform 10 to swing by the linkage of the speed reducer 222, the first driving wheel 23, the first timing belt 25, and the first driven wheel 24, the fulcrum for the magnetic platform 10 to swing is the bearing 26 of the synchro bracket 27. That is, the outer race of the bearing 26 is fixed in the first mounting hole 271, and the inner race of the bearing 26 is fixed to the first rotating shaft 103. When the magnetic platform 10 swings, the first rotating shaft 103 rotates, and the inner race of the bearing 26 is rotated relative to its outer race. As can be understood, by changing the rotation direction of the first motor 21, the magnetic platform 10 can be controlled to swing in the opposite direction with the bearing 26 as the fulcrum, and by controlling the rotation speed of the first motor 21, the swing angle of the magnetic platform 10 can be controlled. Further, after the magnetic platform 10 swings to uniformly shake the cells, the magnetic platform 10 is controlled to swing and stop at a preset tilt angle. In this way, the liquid in the liquid bag 50 after the magnetic beads are sorted is more likely to flow out from the liquid outlet.

[0037] Further, in step S202, the step of controlling the mixing device 2 by the magnetic platform 10 to continuously swing at the mixing angle includes the following steps. Determine the operating parameters of the first motor 21 from the mixing angle. That is, when determining the mixing angle required for the magnetic platform 10, based on previous historical experimental data (the historical experimental data includes the correlation between the historical operating parameters of the first motor 21 and the historical mixing angle) and the above mixing angle, the real-time operating parameters of the first motor 21 can be determined. Specifically, first, determine the historical mixing angle that matches the above mixing angle from the historical experimental data based on the above mixing angle, and use the historical operating parameters of the first motor 21 associated with the determined historical mixing angle as the operating parameters for the subsequent real-time operation of the first motor 21.

[0038] Start the first motor 21, drive the speed reducer 22 with the operating parameters to rotate the first driving wheel 23, rotate the first driven wheel 24 through the first timing belt 25 by the first driving wheel 23, and further swing the magnetic platform around the connection point with the synchro bracket 27. In the present application, first, drive the speed reducer 22 by the first motor 21, and further rotate and drive the first driving wheel 23, the first timing belt 25, and the first driven wheel 24 simultaneously, and swing the magnetic platform 10 around the first rotation axis 103 with the bearing 26 as the fulcrum. Also, the swing angular velocity, swing angle, and angular acceleration of the swing of the magnetic platform 10 correspond one-to-one and match the respective parameters in the above swing parameters. Furthermore, as shown in FIG. 5, the mixing device 2 further includes a two-axis tilt sensor 28 attached to the magnetic platform 10, and the two-axis tilt sensor 28 is used to detect the swing parameters of the magnetic platform 10. Here, the two-axis tilt sensor 28 can detect the swing angle, swing angular velocity, angular acceleration, and the stop angle after the swing is completed in the process of the mixing device 2 swinging to uniformly shake the cells.

[0039] Furthermore, in the step S202, the magnetic platform 10 is controlled by the mixing device 2 to swing continuously for a mixing time at the mixing angle. In the step S30, before performing the waste liquid discharging operation, the following steps are further included. Arrange the magnetic platform 10 horizontally for a preset time, and the preset time can be set to 5 - 20 minutes as required, preferably 10 minutes.

[0040] Obtain a first low-speed swinging parameter including a first low-speed swinging angle and a first low-speed swinging time. The absolute value of the first low-speed swinging angle is smaller than the absolute value of the mixing angle. For example, when the mixing angle is ±25 degrees, the first low-speed swinging angle is ±20 degrees.

[0041] By controlling the magnetic platform 10 by the mixing device 2 to swing at a low speed continuously for a first low-speed swinging time at the first low-speed swinging angle, other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells are suspended in the liquid. In the present application, the magnetic platform 10 can spread the accumulated immunomagnetic bead cells more uniformly by oscillating at the mixing angle, and can also suspend other substances in the liquid bag 50 other than the adsorbed immunomagnetic bead cells in the liquid. On the other hand, in this embodiment, when oscillating at the first low-speed swinging angle, the oscillating force is small, and the immunomagnetic bead cells adsorbed on the magnetic platform 10 are not oscillated. Only other substances attached to the adsorbed immunomagnetic bead cells are oscillated until they are separated from the immunomagnetic bead cells and suspended in the liquid, and thereby are discharged when the waste liquid discharging operation is performed in step S30.

[0042] Furthermore, in the step S30, the step of performing the waste liquid discharging operation includes the following steps. Control the magnetic platform 10 to stop at a preset first stop angle such that the liquid bag 50 inclines downward from the liquid inlet toward the liquid outlet. In this embodiment, when the magnetic platform 10 is swing-controlled according to preset swing parameters to uniformly oscillate the liquid in the liquid bag 50, the preset first stop angle refers to an angle at which the height of the liquid outlet of the liquid bag 50 on the non-moving magnetic platform 10 is lower than the height of the liquid inlet, which makes it easier for the liquid in the liquid bag 50 to flow out from the liquid outlet. Specifically, when the magnetic platform 10 stops at the preset first stop angle, the angle formed between the magnetic platform 10 and the horizontal plane is the preset first stop angle, and the preset first stop angle may be 10 to 30 degrees, preferably 20 degrees.

[0043] Execute a first opening and closing operation to open the waste liquid discharge pipe. Here, the waste liquid discharge pipe includes a pump and a bubble sensor connected between the waste liquid bag 50 and the liquid outlet of the liquid bag 50. That is, the first opening and closing operation means opening all the closed valves on the line of the waste liquid bag 50, the pump, the bubble sensor, and the liquid bag 50 in the waste liquid discharge pipe, and starting the pump (the pump is preferably a peristaltic pump) and the bubble sensor. In this case, the waste liquid discharge pipe is regarded as being opened.

[0044] Discharge the liquid in the liquid bag 50 other than the immunomagnetic bead cells adsorbed through the waste liquid discharge pipe into the waste liquid bag 50. Specifically, the pump in the waste liquid discharge channel sucks the liquid in the liquid bag 50 other than the adsorbed immunomagnetic bead cells into the waste liquid bag 50, and the bubble sensor detects the bubbles in the liquid in the waste liquid discharge pipe and determines whether there is liquid flowing in the waste liquid discharge pipe based on the detection result.

[0045] When it is determined by the bubble sensor that no liquid is passing through the waste liquid discharge pipe, it is determined that the waste liquid discharge is complete. That is, when it is determined that no liquid is passing through the waste liquid discharge pipe, it indicates that all the liquid in the liquid bag 50 has flowed out, and thus, it can be assumed that the waste liquid discharge process is complete.

[0046] Furthermore, in step S40, the step of washing the immunomagnetic bead cells remaining in the liquid bag 50 includes the following steps. Control the magnetic platform 10 to stop at a preset second stop angle such that the liquid bag 50 inclines upward from the liquid inlet toward the liquid outlet. The preset second stop angle and the preset first stop angle are opposite in direction. In this embodiment, the preset second stop angle refers to an angle that makes the height of the liquid outlet of the liquid bag 50 on the stationary magnetic platform 10 higher than the height of the liquid inlet, which is advantageous for the cleaning liquid flowing from the cleaning liquid inlet into the liquid bag 50 through the liquid inlet and then stopping near the liquid inlet and spreading to every corner within the liquid bag 50. Specifically, when the magnetic platform 10 stops at the preset second stop angle, the angle formed between the magnetic platform 10 and the horizontal plane is the preset second stop angle, and the preset second stop angle may be 10 to 30 degrees, preferably 15 to 20 degrees.

[0047] Execute the second opening / closing operation to open the cleaning pipe. The second opening / closing operation includes blocking the passage between the pump and the bubble sensor and the waste liquid bag 50, and connecting the pump and the bubble sensor between the cleaning liquid inlet and the liquid inlet of the liquid bag 50. That is, the second opening / closing operation refers to closing the valve between the pump and the bubble sensor in the waste liquid discharge pipe and the waste liquid bag 50. Ensure that neither the pump nor the bubble sensor is connected to the waste liquid bag 50, and turn off the pump and the bubble sensor, that is, close the waste liquid discharge pipe. At this time, open all the closed valves on the line of the cleaning liquid inlet, pump, bubble sensor, and liquid bag 50, and then start the pump (the pump is preferably a peristaltic pump) and the bubble sensor. In this case, the cleaning pipe is considered to be open.

[0048] Inject a preset first volume of cleaning liquid into the liquid bag 50 through the cleaning pipe. The preset first volume may be 50 - 300 ml, but is preferably 100 ml.

[0049] Obtain a second low-speed oscillation parameter including a second low-speed oscillation angle and a second low-speed oscillation time. Here, the absolute value of the second low-speed oscillation angle is smaller than the absolute value of the mixing angle. For example, when the mixing angle is ±25 degrees, the second low-speed oscillation angle is ±20 degrees.

[0050] After controlling the magnetic platform 10 by the mixing device 2 so as to swing at a low speed for a second low-speed swing time at the second low-speed swing angle, the waste liquid discharging operation is performed again. In the present application, when the magnetic platform 10 swings at a mixing angle, the accumulated immunomagnetic bead cells can be spread more uniformly. However, in this embodiment, when the magnetic platform 10 swings at the second low-speed swing angle, the swinging force is small, and the immunomagnetic bead cells adsorbed on the magnetic platform 10 are not swung. Other substances attached to the adsorbed immunomagnetic bead cells are swung until they are separated from the immunomagnetic bead cells, suspended in the cleaning liquid, and discharged when the waste liquid discharging operation is performed again. By the above cleaning operation, the purity of the adsorbed immunomagnetic bead cells in the finally obtained liquid bag 50 is increased. As can be understood, for the step of performing the waste liquid discharging operation again, reference can be made to the detailed description of the waste liquid discharging operation in the above embodiment, so it will not be described in detail here. However, when performing the waste liquid discharging operation again, in the first opening / closing operation, first, all valves between the pump and the bubble sensor and the cleaning liquid inlet in the cleaning pipe are closed so that neither the pump nor the bubble sensor is connected to the cleaning liquid inlet. Also, the pump and the bubble sensor are closed, that is, the cleaning pipe is closed. At this time, all the closed valves on the line of the waste liquid bag 50, the pump, the bubble sensor, and the liquid bag 50 are opened, and the pump (the pump is preferably a peristaltic pump) and the bubble sensor are activated. In this case, the waste liquid discharging pipe is regarded as being opened again.

[0051] Furthermore, in the step S50, the step of injecting a resuspension into the liquid bag 50 and taking out the liquid bag 50 containing immunomagnetic bead cells includes the following steps. Adjust the magnetic platform 10 to be parallel to the horizontal plane. That is, after performing the waste liquid discharging operation again, in order to facilitate subsequent operations, first, the magnetic platform 10 is restored to the horizontal state.

[0052] Perform the third opening / closing operation to open the resuspension injection pipe. The third opening / closing operation includes blocking the passage between the pump and the bubble sensor and the waste liquid bag 50, and connecting the pump and the bubble sensor between the resuspension inlet and the liquid inlet of the liquid bag 50. That is, the third opening / closing operation means closing the valve between the pump and the bubble sensor and the waste liquid bag 50 in the waste liquid discharge pipe so that neither the pump nor the bubble sensor is connected to the waste liquid bag 50, and closing the pump and the bubble sensor, that is, closing the waste liquid discharge pipe. At this time, open all the closed valves on the line of the resuspension inlet, pump, bubble sensor and liquid bag 50, and then start the pump (the pump is preferably a peristaltic pump) and the bubble sensor. In this case, the resuspension injection pipe is regarded as being open.

[0053] Inject a preset second volume of resuspension into the liquid bag 50 through the resuspension injection pipe. As can be understood, the preset second volume can be set as needed, for example, it is 0 - 300 ml.

[0054] Perform the fourth opening / closing operation. The fourth opening / closing operation includes blocking the passage between the pump and the bubble sensor and the resuspension inlet, and controlling the pump and the bubble sensor to be connected to the atmosphere through the sterile air filter. That is, the fourth opening / closing operation means closing the valve between the pump and the bubble sensor and the resuspension inlet in the resuspension injection pipe so that neither the pump nor the bubble sensor is connected to the resuspension inlet. At this time, open all the closed valves on the line of the sterile air filter, pump, bubble sensor and liquid bag 50. In this case, the resuspension injection pipe is regarded as being open. Furthermore, the liquid bag 50 can be connected to the atmosphere through the pump, the bubble sensor and the sterile air filter. At this time, under atmospheric pressure, all the resuspension remaining in the pipe is discharged into the liquid bag 50 by the action of the pump, so the waste of the expensive resuspension in the pipe is avoided, and the cost of the magnetic bead sorting process is greatly reduced by the above operations in this embodiment.

[0055] When it is determined by the bubble sensor that no liquid is passing through, it is determined that the resuspension has been completely injected into the liquid bag. As can be understood, when the bubble sensor detects bubbles in the pipe and determines from the detection result that no liquid is flowing in the pipe, it can be regarded that all of the resuspension has been injected into the liquid bag 50.

[0056] After hot-sealing the liquid outlet and the liquid inlet of the liquid bag 50, the liquid bag 50 containing the immunomagnetic bead cells is taken out from the magnetic platform 10. That is, in this embodiment, after emptying the pipe, both the liquid outlet and the liquid inlet of the liquid bag 50 are hot-sealed, that is, after hot-sealing the liquid outlet and the liquid inlet using a hot-sealing device, the liquid bag 50 is taken out from the magnetic platform 10. Thereby, the process of magnetic bead sorting is completed, and the immunomagnetic bead cells in the resuspension in the liquid bag 50 are targeted for recovery. At this point, the recovery is completed, and subsequent culture treatment can be carried out.

[0057] Furthermore, the step of injecting a preset second volume of resuspension into the liquid bag 50 through the resuspension injection pipe includes the following steps Inject resuspension into the liquid bag 50 through the resuspension injection pipe. That is, in this embodiment, after opening the resuspension injection pipe, resuspension is injected into the liquid bag 50 through the opened resuspension injection pipe.

[0058] Obtain the weight change information of the liquid bag 50 by the load cell, determine the actual volume of the resuspension injected from the weight change information of the liquid bag 50, or obtain the volume-rotation number data corresponding to the pump, and determine the actual volume of the resuspension injected from the actual rotation number of the pump and the volume-rotation number data. That is, in this embodiment, from the weight change information of the liquid bag 50, determine the difference between the current weight and the original weight of the liquid bag 50, and from this difference and the density of the resuspension, the actual volume of the injected resuspension may be determined. In another embodiment, obtain the history data, that is, the volume of the resuspension injected into the liquid bag 50 for each rotation of the pump obtained in the history test of the pump, that is, the volume-rotation number data, and further obtain the actual rotation number of the rotation of the pump, then the actual volume of the resuspension injected can be determined.

[0059] When the actual volume reaches the preset second volume, it is determined that the resuspension of the preset second volume has been injected into the liquid bag 50. That is, since it is necessary to inject only the resuspension of the preset second volume, when it is determined by the above method that the resuspension of the preset second volume has been injected, it is determined that the resuspension of the preset second volume has been injected into the liquid bag 50, and then steps such as the subsequent fourth opening and closing operation may be executed. As can be understood, in the steps corresponding to the above fourth opening and closing operation, all the resuspension remaining in the resuspension injection pipe can be discharged from the pipe, and since this part of the resuspension is not regarded as the actual volume until the fourth opening and closing operation is executed, the preset second volume can be set to be slightly smaller than the volume of the resuspension to be finally injected into the liquid bag 50 (the difference between the two may be a constant obtained by testing), and in this way, by adding the resuspension discharged from the pipe to the preset second volume, the final volume of the resuspension required for actual injection can be obtained.

[0060] It should be noted that the sequence numbers of each step in the above embodiments do not represent the execution order. The execution order of each process should be determined by its function and internal logic, and it should be understood that the execution process of the embodiments of the present application is not limited.

[0061] The present application also provides a magnetic bead sorting device including a pressing device 1, a mixing device 2, and a controller for executing the above magnetic bead sorting method. The controller is connected to the pressing device 1 and the mixing device 2.

[0062] Specifically, the controller is configured to place an empty liquid bag on a magnetic platform, inject a liquid containing immunomagnetic bead cells into the liquid bag, and adsorb the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform; control the oscillation of the magnetic platform according to preset oscillation parameters to uniformly distribute the immunomagnetic bead cells adsorbed on the magnetic platform, and suspend other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid; perform a waste liquid discharge operation to allow the liquid other than the adsorbed immunomagnetic bead cells in the liquid bag to flow into a waste liquid bag; wash the immunomagnetic bead cells remaining in the liquid bag; inject a resuspension into the liquid bag, take out the liquid bag containing the immunomagnetic bead cells, and confirm that the sorting of the magnetic beads is completed.

[0063] In one embodiment, the step of placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform is Placing the empty liquid bag on the magnetic platform of the pressing device, the pressing device including a closing mechanism attached to the magnetic platform and a pressing mechanism connected to the closing mechanism, Injecting a liquid containing immunomagnetic bead cells into the liquid bag, After closing the closing mechanism, controlling the pressing mechanism to move the closed closing mechanism downward to flatten the liquid bag placed in the accommodation space between the magnetic platform and the closing mechanism, increasing the contact surface between the bottom surface of the liquid bag and the magnetic platform, and further adsorbing the immunomagnetic bead cells in the liquid bag to the contact surface by the magnetic platform.

[0064] In one embodiment, the pressing device further includes a lift mechanism connected to the closing mechanism. Further, after injecting the liquid containing immunomagnetic bead cells into the liquid bag, the controller further, When the closing mechanism cannot be closed because it is pushed up by the inflated liquid bag, controlling the lift mechanism to raise the closing mechanism to increase the accommodation space for placing the liquid bag between the magnetic platform and the closing mechanism, After the closing mechanism rises to a preset closable height, controlling the closing mechanism to close, Controlling the pressing mechanism to move the closed closing mechanism downward to flatten the liquid bag placed in the accommodation space, increasing the contact surface between the bottom surface of the liquid bag and the magnetic platform, and further adsorbing the immunomagnetic bead cells in the liquid bag to the contact surface by the magnetic platform.

[0065] In one embodiment, the step of swing-control the magnetic platform according to the preset swing parameters is, Obtaining preset mixing parameters including a mixing angle and a mixing time; Controlling the magnetic platform by a mixing device to swing continuously for the mixing time at the mixing angle, so that immunomagnetic bead cells are uniformly adsorbed on the magnetic platform, and other substances in the liquid bag other than the adsorbed immunomagnetic bead cells are suspended in the liquid in conjunction with the swing of the magnetic platform.

[0066] In one embodiment, after the step of controlling the magnetic platform by a mixing device to swing continuously for the mixing time at the mixing angle, before performing the waste liquid discharging operation, the controller further Disposing the magnetic platform at a preset horizontal time; Obtaining a first low-speed swing parameter including a first low-speed swing angle and a first low-speed swing time, wherein an absolute value of the first low-speed swing angle is smaller than an absolute value of the mixing angle; Controlling the magnetic platform by the mixing device to swing at a low speed continuously for the first low-speed swing time at the first low-speed swing angle, so as to suspend other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid.

[0067] In one embodiment, the mixing device includes a first motor, a speed reducer, a first driving wheel, a first driven wheel, a first timing belt, and a synchro bracket. The speed reducer is connected to an output shaft of the first motor to reduce the rotation speed of the first motor and increase the output torque. The first driving wheel is connected to a rotating shaft of the speed reducer. The first timing belt is socket-fixed to the first driving wheel and the first driven wheel. The first driven wheel is attached to the magnetic platform. The magnetic platform is rotatably attached to the synchro bracket. The step of controlling the magnetic platform by a mixing device to swing continuously at the mixing angle is Determining the operating parameters of the first motor according to the mixing angle; Starting the first motor, driving the speed reducer with the operating parameters to rotate the first driving wheel, rotationally driving the first driven wheel via the first timing belt by the first driving wheel, and swinging the magnetic platform about the connection point with the synchro bracket.

[0068] In one embodiment, the step of performing the waste liquid discharging operation includes: Controlling the magnetic platform to stop at a preset first stop angle such that the liquid bag inclines downward from the liquid inlet toward the liquid outlet; Performing a first opening / closing operation to open the waste liquid discharge pipe, the waste liquid discharge pipe including a pump and a bubble sensor connected between the waste liquid bag and the liquid outlet of the liquid bag; Discharging the liquid in the liquid bag other than the adsorbed immunomagnetic bead cells into the waste liquid bag through the waste liquid discharge pipe; Determining that the waste liquid discharging is completed when it is determined by the bubble sensor that no liquid is passing through the waste liquid discharge pipe.

[0069] In one embodiment, the step of washing the immunomagnetic bead cells remaining in the liquid bag includes: Controlling the magnetic platform to stop at a preset second stop angle such that the liquid bag inclines upward from the liquid inlet toward the liquid outlet, the preset second stop angle and the preset first stop angle being in opposite directions; Performing a second opening / closing operation to open the washing pipe, the second opening / closing operation blocking the passage between the pump and the bubble sensor and the waste liquid bag and connecting the pump and the bubble sensor between the washing liquid inlet and the liquid inlet of the liquid bag. Injecting a cleaning liquid of a preset first volume into the liquid bag through the cleaning pipe; Obtaining second low-speed oscillation parameters including a second low-speed oscillation angle and a second low-speed oscillation time; After controlling the magnetic platform to oscillate at a low speed for a second low-speed oscillation time at the second low-speed oscillation angle by a mixing device, performing the waste liquid discharging operation.

[0070] In one embodiment, the step of injecting a resuspension into the liquid bag and taking out the liquid bag containing immunomagnetic bead cells includes: Adjusting the magnetic platform to be parallel to the horizontal plane; Performing a third opening / closing operation to open the resuspension injection pipe, where the third opening / closing operation blocks a passage between the pump, the bubble sensor, and the waste liquid bag and connects the pump and the bubble sensor between the resuspension inlet and the liquid inlet of the liquid bag; Injecting a preset second volume of resuspension into the liquid bag through the resuspension injection pipe; Performing a fourth opening / closing operation, where the fourth opening / closing operation includes blocking a passage between the pump, the bubble sensor, and the resuspension inlet and controlling the pump and the bubble sensor to be connected to the atmosphere through a sterile air filter; When it is determined by the bubble sensor that no liquid is passing through, determining that the resuspension has been completely injected into the liquid bag; Heat-sealing the liquid outlet and the liquid inlet of the liquid bag, and then taking out the liquid bag containing immunomagnetic bead cells from the magnetic platform.

[0071] In one embodiment, the step of injecting a preset second volume of resuspension into the liquid bag through the resuspension injection pipe includes: Injecting a resuspension into the liquid bag through the resuspension injection pipe; Obtaining the weight change information of the liquid bag by a load cell, determining the actual volume of the resuspension liquid to be injected from the weight change information of the liquid bag, or obtaining the volume-rotation speed data corresponding to the pump, and determining the actual volume of the resuspension liquid to be injected from the actual rotation speed of the pump and the volume-rotation speed data; When the actual volume reaches the preset second volume, determining that the resuspension liquid of the preset second volume has been injected into the liquid bag.

[0072] For more detailed limitations of the magnetic bead sorting device and the controller, reference can be made to the limitations on the above magnetic bead sorting method, and thus it will not be elaborated here. Each module of the above controller may be implemented in whole or in part by software, hardware, and combinations thereof. Each of the above modules may be incorporated into the processor of a computer device in the form of hardware, or may be independent of this processor. Also, it may be stored in the memory of a computer device in the form of software, called by the processor, and perform operations corresponding to each of the above modules. As can be understood, the controller can be regarded as one or more computer devices. As shown in FIG. 7, the computer device includes a processor, a memory, a network interface, and a database connected via a system bus. The processor of the computer device provides computing and control capabilities. The memory of the computer device includes a readable storage medium and an internal memory. The readable storage medium stores an operating system, computer-readable instructions, and a database. The internal memory provides an environment for the operation of the operating system and computer-readable instructions in the readable storage medium. The database of the computer device is used to store the data used in the magnetic bead sorting method in the above embodiments. The network interface of the computer device is communicably connected to an external terminal via a network. When the computer-readable instructions are executed by the processor, the magnetic bead sorting method is realized. The readable storage medium according to this embodiment includes a non-volatile readable storage medium and a volatile readable storage medium.

[0073] In one embodiment, one or more readable storage media storing computer-readable instructions are provided. The readable storage media according to this embodiment include a non-volatile readable storage media and a volatile readable storage media. The readable storage media store computer-readable instructions, which, when executed by one or more processors, cause the one or more processors to execute the above-described method for sorting magnetic beads.

[0074] Specifically, when the computer-readable instructions are executed by one or more processors, placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform; controlling the oscillation of the magnetic platform according to preset oscillation parameters to uniformly distribute the immunomagnetic bead cells adsorbed on the magnetic platform, and suspending other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid; performing a waste liquid discharge operation to allow the liquid other than the adsorbed immunomagnetic bead cells in the liquid bag to flow into a waste liquid bag; washing the immunomagnetic bead cells remaining in the liquid bag; injecting a resuspension into the liquid bag, taking out the liquid bag containing the immunomagnetic bead cells, and then confirming that the sorting of the magnetic beads is completed, are caused to be executed by one or more processors.

[0075] In one embodiment, the step of placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform is Placing the empty liquid bag on the magnetic platform of the pressing device, wherein the pressing device includes a closing mechanism attached to the magnetic platform and a pressing mechanism connected to the closing mechanism; Injecting a liquid containing immunomagnetic bead cells into the liquid bag; After closing the closing mechanism, controlling the pressing mechanism to move the closed closing mechanism downward to flatly press the liquid bag placed in the accommodation space between the magnetic platform and the closing mechanism, increasing the contact surface between the bottom surface of the liquid bag and the magnetic platform, and further adsorbing the immunomagnetic bead cells in the liquid bag to the contact surface by the magnetic platform.

[0076] In one embodiment, the pressing device further includes a lift mechanism connected to the closing mechanism. Further, after injecting the liquid containing immunomagnetic bead cells into the liquid bag, when the computer-readable instructions are executed by one or more processors, If the closing mechanism cannot be closed because it is pushed up by the inflated liquid bag, controlling the lift mechanism to raise the closing mechanism to increase the accommodation space for placing the liquid bag between the magnetic platform and the closing mechanism; After the closing mechanism rises to a preset closable height, controlling the closing mechanism to close; Controlling the pressing mechanism to move the closed closing mechanism downward to flatly press the liquid bag placed in the accommodation space, increasing the contact surface between the bottom surface of the liquid bag and the magnetic platform, and further adsorbing the immunomagnetic bead cells in the liquid bag to the contact surface by the magnetic platform, and causing one or more processors to execute.

[0077] In one embodiment, the step of controlling the swinging of the magnetic platform according to the preset swinging parameters is Obtaining preset mixing parameters including a mixing angle and a mixing time; By controlling the magnetic platform by a mixing device to swing continuously for the mixing time at the mixing angle, immune magnetic bead cells are uniformly adsorbed onto the magnetic platform, and other substances in the liquid bag other than the adsorbed immune magnetic bead cells are suspended in the liquid in conjunction with the swing of the magnetic platform. The method includes the steps of:

[0078] In one embodiment, after the step of controlling the magnetic platform by a mixing device to swing continuously for the mixing time at the mixing angle, before performing the waste liquid discharging operation, when the computer-readable instructions are executed by one or more processors, Placing the magnetic platform at a preset horizontal time; Obtaining first low-speed swing parameters including a first low-speed swing angle and a first low-speed swing time, wherein an absolute value of the first low-speed swing angle is smaller than an absolute value of the mixing angle; By controlling the magnetic platform by the mixing device to swing at a low speed continuously for the first low-speed swing time at the first low-speed swing angle, other substances in the liquid bag other than the adsorbed immune magnetic bead cells are suspended in the liquid. Let one or more processors execute the steps of:

[0079] In one embodiment, the mixing device includes a first motor, a speed reducer, a first driving wheel, a first driven wheel, a first timing belt, and a synchro bracket. The speed reducer is connected to an output shaft of the first motor to reduce the rotation speed of the first motor and increase the output torque. The first driving wheel is connected to a rotating shaft of the speed reducer. The first timing belt is socket-fixed to the first driving wheel and the first driven wheel. The first driven wheel is attached to the magnetic platform, and the magnetic platform is rotatably attached to the synchro bracket. The step of controlling the magnetic platform by a mixing device to swing continuously at the mixing angle includes: Determining the operating parameters of the first motor according to the mixing angle; Starting the first motor, driving the speed reducer with the operating parameters to rotate the first driving wheel, rotationally driving the first driven wheel through the first timing belt by the first driving wheel, and swinging the magnetic platform around the connection point with the synchro bracket.

[0080] In one embodiment, the step of performing the waste liquid discharging operation includes: Controlling the magnetic platform to stop at a preset first stop angle such that the liquid bag inclines downward from the liquid inlet towards the liquid outlet; Performing a first opening / closing operation to open the waste liquid discharge pipe, where the waste liquid discharge pipe includes a pump and a bubble sensor connected between the waste liquid bag and the liquid outlet of the liquid bag; Discharging the liquid in the liquid bag other than the adsorbed immunomagnetic bead cells into the waste liquid bag through the waste liquid discharge pipe; When it is determined by the bubble sensor that no liquid is passing through the waste liquid discharge pipe, determining that the waste liquid discharge is complete.

[0081] In one embodiment, the step of washing the immunomagnetic bead cells remaining in the liquid bag includes: Controlling the magnetic platform to stop at a preset second stop angle such that the liquid bag inclines upward from the liquid inlet towards the liquid outlet, where the preset second stop angle and the preset first stop angle are in opposite directions; Performing a second opening / closing operation to open the washing pipe, where the second opening / closing operation blocks the passage between the pump and the bubble sensor and the waste liquid bag, and connects the pump and the bubble sensor between the washing liquid inlet and the liquid inlet of the liquid bag; Injecting a cleaning liquid of a preset first volume into the liquid bag through the cleaning pipe; Obtaining a second low-speed oscillation parameter including a second low-speed oscillation angle and a second low-speed oscillation time; After controlling the magnetic platform to oscillate at a low speed for a second low-speed oscillation time at the second low-speed oscillation angle by a mixing device, performing the waste liquid discharging operation.

[0082] In one embodiment, the step of injecting a resuspension into the liquid bag and taking out the liquid bag containing immunomagnetic bead cells includes: Adjusting the magnetic platform to be parallel to the horizontal plane; Performing a third opening / closing operation to open the resuspension injection pipe, where the third opening / closing operation cuts off the passage between the pump, the bubble sensor, and the waste liquid bag, and connects the pump and the bubble sensor between the resuspension inlet and the liquid inlet of the liquid bag; Injecting a preset second volume of resuspension into the liquid bag through the resuspension injection pipe; Performing a fourth opening / closing operation, where the fourth opening / closing operation includes cutting off the passage between the pump, the bubble sensor, and the resuspension inlet, and controlling the pump and the bubble sensor to be connected to the atmosphere through a sterile air filter; When it is determined by the bubble sensor that no liquid is passing through, determining that the resuspension has been completely injected into the liquid bag; After heat-sealing the liquid outlet and the liquid inlet of the liquid bag, taking out the liquid bag containing immunomagnetic bead cells from the magnetic platform.

[0083] In one embodiment, the step of injecting a preset second volume of resuspension into the liquid bag through the resuspension injection pipe includes: Injecting a resuspension into the liquid bag through the resuspension injection pipe; Obtaining the weight change information of the liquid bag by a load cell, determining the actual volume of the resuspension solution to be injected from the weight change information of the liquid bag, or obtaining the volume-rotation speed data corresponding to the pump, and determining the actual volume of the resuspension solution to be injected from the actual rotation speed of the pump and the volume-rotation speed data; Determining that the resuspension solution with the preset second volume has been injected into the liquid bag when the actual volume reaches the preset second volume.

[0084] Regarding the specific limitations of the readable storage medium, reference can be made to the limitations of the above magnetic bead sorting method, so it will not be described in detail here.

[0085] Those skilled in the art can understand that when stored in a non-volatile readable storage medium or a volatile readable storage medium and executed, the computer-readable instructions including the flow of the embodiments of the above methods can instruct the relevant hardware to realize all or part of the flow in the methods of the above embodiments. References to memory, storage, database, or other media used in each embodiment of the present application may include non-volatile and / or volatile memory. Non-volatile memory may include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of example, and not limitation, RAM is available in various forms such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual data rate SDRAM (DDRSDRAM), extended SDRAM (ESDRAM), synchronous link (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (RDRAM), and memory bus dynamic RAM (RDRAM).

[0086] For the sake of convenience and brevity of description, only the division of the above functional units and modules is taken as an example for description. However, those skilled in the art can, in actual applications, divide the above functions into different functional units and modules as needed. That is, it can be clearly understood that the internal structure of the device can be divided into different functional units and modules to complete all or part of the above functions.

[0087] The above embodiments are only used for explaining the technical solutions of the present application and do not limit it. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions described in the foregoing embodiments can be modified or some of their technical features can be equivalently replaced. However, these modifications or replacements do not deviate from the spirit and scope of the technical solutions of the corresponding technical solutions of the embodiments of the present application, and all should be included in the protection scope of the present application.

Description of Reference Signs

[0088] The description of the reference signs in the specification is as follows. 1 Pressing device 10 Magnetic platform 101 Base 1011 Lift through hole 1012 Guide through hole 102 Magnetic block 103 First rotating shaft 20 Lift mechanism 201 Lifting assembly 2011 First lifting block 2012 Second lifting block 2013 Limiting plate 2014 Rotating shaft 202 Lift rod 203 Lifting drive assembly 2031 Second motor 2032 Second driving wheel 2033 Second driven wheel 2034 Second Timing Belt 2035 Second Rotating Shaft 2036 Cam 2037 Mounting Bearing 2038 Cam Carrier 2039 Second Mounting Hole 2040 Bearing Follower 2041 Fixed Plate 2042 Sensing Block 2043 Photoelectric Sensor 30 Closing Mechanism 301 Cover 302 First Adsorbing Part 303 Second Adsorbing Part 40 Pressing Mechanism 401 Guide Shaft 402 Spring 403 Linear Bearing 4011 Stopper 50 Liquid Bag 2 Mixing Device 21 First Motor 22 Speed Reducer 23 First Driving Wheel 24 First Driven Wheel 25 First Timing Belt 26 Bearing 27 Synchronous Bracket 271 First Mounting Hole 28 Two-Axis Tilt Sensor

Claims

1. A method for sorting magnetic beads, comprising: placing an empty liquid bag on a magnetic platform, injecting a liquid containing immunomagnetic bead cells into the liquid bag, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform; controlling the oscillation of the magnetic platform according to preset oscillation parameters to uniformly distribute the immunomagnetic bead cells adsorbed on the magnetic platform, and suspending other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid; performing a waste liquid discharge operation to allow the liquid other than the adsorbed immunomagnetic bead cells in the liquid bag to flow into a waste liquid bag; washing the immunomagnetic bead cells remaining in the liquid bag; injecting a resuspension into the liquid bag, taking out the liquid bag containing the immunomagnetic bead cells, and confirming that the sorting of the magnetic beads is completed.

2. The step of placing the empty liquid bag on the magnetic platform and injecting the liquid containing immunomagnetic bead cells into the liquid bag, and adsorbing the immunomagnetic bead cells in the liquid in the liquid bag by the magnetic platform includes: placing the empty liquid bag on the magnetic platform of the pressing device, the pressing device including a closing mechanism attached to the magnetic platform and a pressing mechanism connected to the closing mechanism; injecting a liquid containing immunomagnetic bead cells into the liquid bag; after the closing mechanism is closed, controlling the pressing mechanism to move the closed closing mechanism downward to flatten the liquid bag placed in the accommodation space between the magnetic platform and the closing mechanism, increasing the contact surface between the bottom surface of the liquid bag and the magnetic platform, and further adsorbing the immunomagnetic bead cells in the liquid bag to the contact surface by the magnetic platform. The method for sorting magnetic beads according to Claim 1.

3. The pressing device further includes a lift mechanism connected to the closing mechanism. After injecting the liquid containing the immunomagnetic bead cells into the liquid bag, When the closing mechanism cannot be closed because it is pushed up by the inflated liquid bag, by controlling the lift mechanism to raise the closing mechanism, increasing the accommodation space for arranging the liquid bag between the magnetic platform and the closing mechanism; After the closing mechanism has risen to a preset closable height, controlling the closing mechanism to close; By controlling the pressing mechanism to move the closed closing mechanism downward, flattening the liquid bag placed in the accommodation space, increasing the contact surface between the bottom surface of the liquid bag and the magnetic platform, and further adsorbing the immunomagnetic bead cells in the liquid bag to the contact surface by the magnetic platform. The method for sorting magnetic beads according to claim 2 further includes the steps of:

4. The step of controlling the rocking of the magnetic platform according to the preset rocking parameters includes: Obtaining preset mixing parameters including a mixing angle and a mixing time; By controlling the magnetic platform by a mixing device to rock continuously for the mixing time at the mixing angle, uniformly adsorbing immunomagnetic bead cells on the magnetic platform, and suspending other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid in conjunction with the rocking of the magnetic platform. The method for sorting magnetic beads according to claim 1 includes the steps of:

5. After the step of controlling the magnetic platform by a mixing device to rock continuously for the mixing time at the mixing angle, before performing the waste liquid discharging operation, Placing the magnetic platform horizontally for a preset time; Obtaining a first low-speed rocking parameter including a first low-speed rocking angle and a first low-speed rocking time, wherein the absolute value of the first low-speed rocking angle is smaller than the absolute value of the mixing angle; By controlling the magnetic platform by the mixing device to rock at a low speed continuously for the first low-speed rocking time at the first low-speed rocking angle, suspending other substances in the liquid bag other than the adsorbed immunomagnetic bead cells in the liquid. The method for sorting magnetic beads according to claim 4 further includes the steps of:

6. The mixing device includes a first motor, a speed reducer, a first driving wheel, a first driven wheel, a first timing belt, and a synchro bracket. The speed reducer is connected to the output shaft of the first motor to reduce the rotation speed of the first motor and increase the output torque. The first driving wheel is connected to the rotating shaft of the speed reducer. The first timing belt is socket-fixed to the first driving wheel and the first driven wheel. The first driven wheel is attached to the magnetic platform, and the magnetic platform is rotatably attached to the synchro bracket. The step of controlling the magnetic platform by the mixing device to continuously swing at the mixing angle is the step of determining the operating parameters of the first motor according to the mixing angle, and starting the first motor, driving the speed reducer with the operating parameters to rotate the first driving wheel, rotationally driving the first driven wheel through the first timing belt by the first driving wheel, and swinging the magnetic platform around the connection point with the synchro bracket. The method for sorting magnetic beads according to claim 4 includes the above steps.

7. The step of performing the waste liquid discharging operation is controlling the magnetic platform to stop at a preset first stop angle so that the liquid bag inclines downward from the liquid inlet towards the liquid outlet, and performing a first opening and closing operation to open the waste liquid discharge pipe, where the waste liquid discharge pipe includes a pump and a bubble sensor connected between the waste liquid bag and the liquid outlet of the liquid bag. discharging the liquid in the liquid bag other than the adsorbed immunomagnetic bead cells into the waste liquid bag through the waste liquid discharge pipe, and when it is determined by the bubble sensor that no liquid is passing through the waste liquid discharge pipe, determining that the waste liquid discharge is completed. The method for sorting magnetic beads according to claim 1 includes the above steps.

8. The step of washing the immunomagnetic bead cells remaining in the liquid bag is controlling the magnetic platform to stop at a preset second stop angle so that the liquid bag inclines upward from the liquid inlet towards the liquid outlet, where the preset second stop angle and the preset first stop angle are in opposite directions. Performing a second opening / closing operation to open the cleaning pipe, wherein the second opening / closing operation includes blocking a passage between the pump and the bubble sensor and the waste liquid bag, and connecting the pump and the bubble sensor between the cleaning liquid inlet and the liquid inlet of the liquid bag, the step; Injecting a preset first volume of cleaning liquid into the liquid bag through the cleaning pipe; Obtaining a second low-speed oscillation parameter including a second low-speed oscillation angle and a second low-speed oscillation time; Controlling the magnetic platform by a mixing device to oscillate at a low speed for a second low-speed oscillation time at the second low-speed oscillation angle, and then performing the waste liquid discharging operation, the method for sorting magnetic beads according to claim 7.

9. The step of injecting a resuspension into the liquid bag and taking out the liquid bag containing immunomagnetic bead cells; Adjusting the magnetic platform to be parallel to the horizontal plane; Performing a third opening / closing operation to open the resuspension injection pipe, wherein the third opening / closing operation includes blocking a passage between the pump and the bubble sensor and the waste liquid bag, and connecting the pump and the bubble sensor between the resuspension inlet and the liquid inlet of the liquid bag, the step; Injecting a preset second volume of resuspension into the liquid bag through the resuspension injection pipe; Performing a fourth opening / closing operation, wherein the fourth opening / closing operation includes blocking a passage between the pump and the bubble sensor and the resuspension inlet, and controlling the pump and the bubble sensor to be connected to the atmosphere through a sterile air filter, the step; When it is determined by the bubble sensor that no liquid is passing through, determining that the resuspension has been completely injected into the liquid bag; Heat-sealing the liquid outlet and the liquid inlet of the liquid bag, and then taking out the liquid bag containing immunomagnetic bead cells from the magnetic platform, the method for sorting magnetic beads according to claim 7.

10. The step of injecting a preset second volume of resuspension into the liquid bag through the resuspension injection pipe; Injecting a resuspension into the liquid bag through the resuspension injection pipe; Obtaining the weight change information of the liquid bag by a load cell, determining the actual volume of the resuspension to be injected from the weight change information of the liquid bag, or obtaining the volume-rotation speed data corresponding to the pump, and determining the actual volume of the resuspension to be injected from the actual rotation speed of the pump and the volume-rotation speed data; When the actual volume reaches the preset second volume, determining that the resuspension of the preset second volume has been injected into the liquid bag, the magnetic bead sorting method according to claim 9, comprising:

11. A magnetic bead sorting device, Comprising a pressing device, a mixing device, and a controller for executing the magnetic bead sorting method according to any one of claims 1 to 10, wherein the controller is connected to the pressing device and the mixing device, the magnetic bead sorting device.

12. A computer-readable storage medium, When executed by a processor, a computer-readable storage medium storing computer-readable instructions for realizing the magnetic bead sorting method according to any one of claims 1 to 10.

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