Liposuction tissue lavage system and method

Abstract

The present disclosure relates to a system for cleaning liposuction tissue, the system comprising: a chamber; a lid connected to the upper side of the chamber, the lid including a collection port configured to connect to a hose and receive liposuction tissue collected from the hose; a subassembly including a mesh basket, the subassembly being positioned within the upper part of the chamber and configured to drain the liposuction tissue through the mesh basket; a tray located in the lower part of the chamber, the tray including a foam assembly configured to concentrate the drained liposuction tissue; and an extraction port along a sidewall of the chamber, configured to allow extraction of the concentrated liposuction tissue from the system.

Description

[Technical Field]

[0001] The present disclosure, according to some embodiments, relates to a system for cleansing lipoaspirate for use in fat grafting procedures, and methods of using the system. [Background technology]

[0002] Fat grafting is a common procedure in which adipose tissue (otherwise known as body fat) is removed from one area of ​​the body via liposuction, processed, and then transferred to another area of ​​the body. This technique is used in a variety of reconstructive and aesthetic cases. Some areas where fat grafting is typically used include the face, chest, waist, buttocks, hands, and feet, although many more areas for different applications are currently being explored.

[0003] When adipose tissue is harvested, it often contains, in addition to fat cells (adipocytes), other agents such as water or other fluids, free lipids, blood cells, etc. It has been discovered that removing such impurities and / or fluids from the harvested adipose tissue before reintroducing the fat cells into the patient's body can be beneficial to the success of the transplant.

[0004] Fat transplantation procedures generally involve three steps: (1) extracting fat from a patient's donor site(s); (2) processing the fat, which involves removing excess tumescent fluid, oil, and blood, thereby leaving separated adipose tissue; and (3) reinjecting the processed fat into a target site within the patient. Typically, devices use decantation, centrifugation, and washing steps during the fat processing stage (step 2) to purify the liposuction tissue, resulting in a high percentage of viable adipose tissue for transplantation purposes. Several procedures for processing fat have been developed, such as those described in U.S. Pat. No. 8,512,695, which is incorporated herein by reference. The '695 patent describes a process for adding poloxamer 188 (P188) to harvested fat to improve cell viability.

[0005] To achieve this separation, or "purification," of fat, several procedures and systems have been developed. For example, U.S. Patent No. 10,118,140, ​​incorporated herein by reference, describes a processing system that includes two chambers, one for collecting lipoaspirate tissue and the other for processing the lipoaspirate tissue. See Figure 8A of the '140 patent, which shows this configuration.

[0006] The use of separate chambers, as found in these known liposuction tissue lavage systems, may be disliked by some surgeons due to usability issues and the desirability of having a closed or semi-closed system when possible. Improvements to existing multi-chamber systems are desirable. Improved methods of manufacturability are also desirable. Summary of the Invention

[0007] The present disclosure describes a system for cleaning liposuction tissue, including a chamber with a hollow body formed with a sidewall having an outer surface and an inner surface, the chamber being open at the top and bottom sides of the chamber. The system may include a lid having a top surface, a lip extending downward from the bottom surface of the lid and configured to securely connect the lid to the top side of the chamber, and a harvest port including a cylindrical tap extending outward from the top side of the lid and configured to connect to a hose and receive harvested liposuction tissue from the hose. The system may include a subassembly including a mesh basket disposed within the upper part of the chamber and configured to receive liposuction tissue received through the harvest port and separate waste fluid extracted from the liposuction tissue to form cleansed liposuction tissue. The system may include a tray configured to be inserted into the lower part of the chamber through the opening in the sidewall, thereby forming the bottom of the system. The tray may include a plate, a raised sidewall disposed along the entire periphery of the plate and extending upward from the top surface of the plate, and a foam assembly fitted over the plate and within the sidewall to be disposed within the lower portion of the chamber, the foam assembly configured to concentrate the washed lipoaspirate tissue. The system may include an extraction port along one of the sidewalls of the chamber configured to allow removal of one of the washed lipoaspirate tissue and the concentrated lipoaspirate tissue.

[0008] In some embodiments, the extraction port may include a cylindrical hole in the bottom of the chamber, protruding outward from the side of the chamber. The extraction port may include threads configured to couple to an extraction port cap that contains the extract within the system. The lid may include a compartment for housing at least one of a spatula and a cleaning packet. The system may include a removable separator disposed on an upper surface of the raised sidewall of the tray, the removable separator configured to separate the contents contained within the chamber from the tray. In some embodiments, the separator may include a gasket configured to form a seal between the removable separator and the chamber, and a handle protruding outward from the side of the removable separator, and thus outward from the chamber, configured to be pulled to remove the removable separator from the chamber. The foam assembly may include multiple foam members forming a complex channel system configured to redistribute fluid from the liposuction tissue throughout the foam assembly. The foam assembly may be configured to prevent the foam members from becoming saturated before they can concentrate the liposuction tissue.

[0009] In some embodiments, the system may include a lid. The lid may include an opening on a top surface of the lid configured to allow access to the interior of the mesh basket. The mesh basket may include a mesh bottom forming the bottom of the subassembly and a plurality of mesh sides forming the sides of the subassembly. The tray may include a squeegee. The lid may include a port cap that fits into the opening on the top surface of the lid and is configured to be removed from the opening to allow a user to access the interior of the chamber with a spatula. The subassembly may include a circular hole in a bottom portion of the subassembly that aligns with the extraction port of the chamber. The circular hole may be configured to form a seal with the extraction port via a slit valve.

[0010] According to some embodiments, the present disclosure relates to a method for cleaning harvested lipoaspirate tissue using a lipoaspirate tissue cleaning system. The method may include receiving the harvested lipoaspirate tissue into a chamber of the lipoaspirate tissue cleaning system such that the harvested lipoaspirate tissue is contained by a mesh basket disposed in an upper portion of the chamber to form a received lipoaspirate tissue. The method may include adding a cleaning fluid to the chamber and mixing the cleaning fluid with the received lipoaspirate tissue using a spatula to form a mixed lipoaspirate tissue. The method may include separating the mixed lipoaspirate tissue into extracted waste fluid and washed lipoaspirate tissue.

[0011] The method may include removing a removable separator from the liposuction tissue washing system so that the washed liposuction tissue can contact a foam assembly contained within a tray contained within a lower portion of the chamber, the foam assembly being positioned below the mesh basket. The method may include absorbing a portion of the extracted waste fluid into the foam assembly to concentrate the washed liposuction tissue to form concentrated liposuction tissue. In some embodiments, one of the washed liposuction tissue and the concentrated liposuction tissue is removed from the liposuction tissue washing system through an extraction port located within a lower portion of the sidewall of the chamber. The concentrated liposuction tissue may have a liposuction tissue concentration per unit volume that is about 2 to about 100 times higher than the harvested liposuction tissue.

[0012] The method may include attaching a collection port to a liposuction cannula. In some embodiments, adding a washing solution to the chamber may include pouring the washing solution through an opening in the lid that tightly connects to the top of the chamber via a lip extending downward from the bottom surface of the lid. Adding the washing solution to the chamber may be performed at a ratio of washing solution to the received lipoaspirate tissue ranging from about 1:4 to about 4:1. The mixed lipoaspirate tissue may be allowed to stand for a time ranging from about 30 seconds to about 5 minutes. Mixing the washing solution and the received lipoaspirate tissue with a spatula may be performed for a time ranging from about 1 minute to about 10 minutes. The washing solution may include a biocompatible block copolymer including repeating units of polyethylene oxide (PEO) and polypropylene oxide (PPO).

[0013] The method may include receiving additional harvested lipoaspirate tissue into a chamber of the lipoaspirate tissue washing system such that the additional harvested lipoaspirate tissue is contained by a mesh basket disposed within an upper portion of the chamber to form additional received lipoaspirate tissue. The method may include adding additional washing fluid to the chamber and mixing the washing fluid with the received lipoaspirate tissue with a spatula to form additional mixed lipoaspirate tissue. The method may include separating additional effluent from the additional mixed lipoaspirate tissue to form additional extracted effluent and additional washed lipoaspirate tissue, the extracted effluent being removed from the lipoaspirate tissue washing system through an extraction port disposed in a lower portion of a sidewall of the chamber.

[0014] Some embodiments of the present disclosure can be understood in part by reference to this disclosure and the accompanying drawings. [Brief explanation of the drawings]

[0015] [Figure 1] 1 illustrates an exploded view of the disclosed liposuction tissue lavage system, according to an exemplary embodiment of the present disclosure. [Figure 2A] 1 illustrates a top view of a liposuction tissue lavage system according to an exemplary embodiment of the present disclosure; [Figure 2B] 2B shows an isometric view of the liposuction tissue lavage system of FIG. 2A according to an exemplary embodiment of the present disclosure. [Figure 3A] 2B illustrates a top view of the liposuction tissue lavage system of FIG. 2A connected to a harvesting hose, according to an exemplary embodiment of the present disclosure. [Figure 3B] 2B illustrates a top view of the liposuction tissue lavage system of FIG. 2A with the port cap removed, according to an exemplary embodiment of the present disclosure. [Figure 3C] 2B illustrates a top view of the liposuction tissue lavage system of FIG. 2A with the lid removed, according to an exemplary embodiment of the present disclosure. [Figure 4A] FIG. 1 illustrates a top view of a foam configuration having channels running along the periphery according to an exemplary embodiment of the present disclosure. [Figure 4B] 4B illustrates a top view of the foam tray of FIG. 4A with a channel system and foam according to an exemplary embodiment of the present disclosure. [Figure 5] FIG. 1 illustrates a top view of a foam assembly having channels according to an exemplary embodiment of the present disclosure. [Figure 6A] FIG. 1 illustrates a side view of a removable separator according to an exemplary embodiment of the present disclosure. [Figure 6B] FIG. 6B shows a top view of the removable separator of FIG. 6A according to an exemplary embodiment of the present disclosure. [Figure 6C] 6B shows an isometric view of the removable separator of FIG. 6A according to an exemplary embodiment of the present disclosure. [Figure 7A] 1 illustrates an isometric view of a squeegee according to an exemplary embodiment of the present disclosure. [Figure 7B] FIG. 7B illustrates a side view of the squeegee from FIG. 7A according to an exemplary embodiment of the present disclosure. [Figure 7C] 7B illustrates another side view of the squeegee from FIG. 7A according to an exemplary embodiment of the present disclosure. [Figure 7D] 7B shows a top view of the squeegee from FIG. 7A. [Figure 8] FIG. 1 illustrates a top view of a subassembly according to an exemplary embodiment of the present disclosure. [Figure 9A]FIG. 1 illustrates a side view of a chamber according to an exemplary embodiment of the present disclosure. [Figure 9B] 9B shows a top view of the chamber from FIG. 9A according to an exemplary embodiment of the present disclosure. [Figure 10] 2B illustrates a side view of the liposuction tissue lavage system of FIG. 2A having a slightly tiled chamber, according to an exemplary embodiment of the present disclosure. [Figure 11] 1 illustrates an envelope-type containment system for the disclosed liposuction tissue lavage system, according to an exemplary embodiment of the present disclosure. [Figure 12] 1 illustrates a box-type storage system for the disclosed liposuction tissue lavage system, according to an exemplary embodiment of the present disclosure. [Figure 13A] 2B shows a front view of the liposuction tissue lavage system of FIG. 2A according to an exemplary embodiment of the present disclosure. [Figure 13B] 2B illustrates a top view of the liposuction tissue lavage system of FIG. 2A according to an exemplary embodiment of the present disclosure. [Figure 13C] 2B shows a side view of the liposuction tissue lavage system of FIG. 2A according to an exemplary embodiment of the present disclosure. [Figure 14A] 1 illustrates an isometric view of a subassembly and squeegee mating with a chamber according to an exemplary embodiment of the present disclosure. [Figure 14B] 14B shows another isometric view of the chamber of FIG. 14A according to an exemplary embodiment of the present disclosure. [Figure 14C] 14B shows a cross-sectional view of the chamber of FIG. 14A according to an exemplary embodiment of the present disclosure. [Figure 15A] FIG. 1 illustrates a top view of a foam assembly according to an exemplary embodiment of the present disclosure. [Figure 15B] 15B shows an exploded view of the foam assembly of FIG. 15A according to an exemplary embodiment of the present disclosure. [Figure 15C] FIG. 15B shows a side view of the foam assembly of FIG. 15A according to an exemplary embodiment of the present disclosure. [Figure 16A] 1 illustrates an isometric view of a port cap according to an exemplary embodiment of the present disclosure. [Figure 16B]16B shows a top view of the port cap of FIG. 16A according to an exemplary embodiment of the present disclosure. [Figure 16C] 16B shows a cross-sectional side view of the port cap of FIG. 16A according to an exemplary embodiment of the present disclosure. [Figure 16D] 16B shows a side view of the port cap of FIG. 16A according to an exemplary embodiment of the present disclosure. [Figure 16E] 16B shows a cross-sectional front view of the port cap of FIG. 16A according to an exemplary embodiment of the present disclosure. [Figure 16F] 16B shows a cross-sectional side view of the port cap of FIG. 16A according to an exemplary embodiment of the present disclosure. [Figure 17A] 1 illustrates a top view of a lid according to an exemplary embodiment of the present disclosure. [Figure 17B] 17B shows a top isometric view of the lid of FIG. 17A according to an exemplary embodiment of the present disclosure. [Figure 17C] 17B shows a cross-sectional side view of the lid of FIG. 17A according to an exemplary embodiment of the present disclosure. [Figure 17D] 17B shows a side view of the lid of FIG. 17A according to an exemplary embodiment of the present disclosure. [Figure 17E] FIG. 17B shows a bottom view of the lid of FIG. 17A according to an exemplary embodiment of the present disclosure. [Figure 17F] 17B shows a bottom isometric view of the lid of FIG. 17A according to an exemplary embodiment of the present disclosure. [Figure 18A] 1 illustrates a top view of a lid according to an exemplary embodiment of the present disclosure. [Figure 18B] 18B shows a top isometric view of the lid of FIG. 18A according to an exemplary embodiment of the present disclosure. [Figure 18C] 18B shows a side view of the lid of FIG. 18A according to an exemplary embodiment of the present disclosure. [Figure 19A] 1 illustrates an isometric view of a chamber according to an exemplary embodiment of the present disclosure. [Figure 19B] 19B shows a top view of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 19C] 19B shows a cross-sectional side view of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 19D]19B shows a front view of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 19E] 19B shows a side view of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 19F] 19B shows a cross-sectional view of the end of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 19G] FIG. 19B shows a bottom view of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 19H] 19B shows a cross-sectional side view of the chamber of FIG. 19A according to an exemplary embodiment of the present disclosure. [Figure 20A] 1 illustrates a cross-sectional front view of a chamber according to an exemplary embodiment of the present disclosure. [Figure 20B] 20B shows a top view of the chamber of FIG. 20A according to an exemplary embodiment of the present disclosure. [Figure 20C] 20B shows a cross-sectional rear view of the chamber of FIG. 20A according to an exemplary embodiment of the present disclosure. [Figure 20D] FIG. 20B shows a front view of the chamber of FIG. 20A with a tray installed, according to an exemplary embodiment of the present disclosure. [Figure 20E] FIG. 20B shows a front view of the chamber of FIG. 20A without a tray installed, according to an exemplary embodiment of the present disclosure. [Figure 21A] FIG. 1 illustrates a side view of a chamber according to an exemplary embodiment of the present disclosure. [Figure 21B] FIG. 21B shows a front view of the chamber of FIG. 21A with a tray installed, according to an exemplary embodiment of the present disclosure. [Figure 21C] FIG. 21B shows another side view of the chamber of FIG. 21A with a tray installed, according to an exemplary embodiment of the present disclosure. [Figure 21D] FIG. 21B shows a rear view of the chamber of FIG. 21A according to an exemplary embodiment of the present disclosure. [Figure 22A] 1 illustrates an isometric view of a subassembly according to an exemplary embodiment of the present disclosure. [Figure 22B] 22B shows a top view of the subassembly of FIG. 22A according to an exemplary embodiment of the present disclosure. [Figure 22C]22B shows a front view of the subassembly of FIG. 22A according to an exemplary embodiment of the present disclosure. [Figure 22D] 22B shows a side view of the subassembly of FIG. 22A according to an exemplary embodiment of the present disclosure. [Figure 22E] 22B illustrates a rear view of the subassembly of FIG. 22A according to an exemplary embodiment of the present disclosure. [Figure 22F] 22B shows another side view of the subassembly of FIG. 22A according to an alternative embodiment of the present disclosure. [Figure 23A] 1 illustrates an isometric view of a tray according to an exemplary embodiment of the present disclosure. [Figure 23B] 23B shows a top view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23C] 23B shows a side view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23D] 23B illustrates a top isometric view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23E] 23B shows a front view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23F] 23B shows a cross-sectional front view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23G] FIG. 23B shows a bottom view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23H] 23B shows an enlarged view of a portion of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23I] 23B shows a cross-sectional front view of the tray of FIG. 23A according to an exemplary embodiment of the present disclosure. [Figure 23J] FIG. 23I shows a close-up view of a corner of the front cross-sectional view shown in FIG. 23I, according to an exemplary embodiment of the present disclosure. [Figure 24A] FIG. 1 illustrates a side view of a removable separator according to an exemplary embodiment of the present disclosure. [Figure 24B] FIG. 24B shows another side view of the removable separator of FIG. 24A according to an exemplary embodiment of the present disclosure. [Figure 24C]24B shows an isometric view of the removable separator of FIG. 24A according to an exemplary embodiment of the present disclosure. [Figure 24D] 24B shows a top view of the removable separator of FIG. 24A according to an exemplary embodiment of the present disclosure. [Figure 24E] FIG. 24B illustrates a bottom isometric view of the removable separator of FIG. 24A according to an exemplary embodiment of the present disclosure. [Figure 24F] FIG. 24B shows a bottom view of the removable separator of FIG. 24A according to an exemplary embodiment of the present disclosure. [Figure 24G] 24B shows a front view of the removable separator of FIG. 24A according to an exemplary embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0016] The present disclosure relates to a liposuction tissue lavage system and a method for lavaging harvested liposuction tissue using the lavage system. The disclosed liposuction tissue lavage system advantageously includes a single chamber structure that allows for both collection and processing of liposuction tissue. The disclosed liposuction tissue lavage system can be used in an operating room to aspirate, harvest, lavage, filter, and transport adipose tissue for reinjection into the same patient from whom the liposuction tissue was harvested, with no additional processing required. The disclosed system and method can be used in a variety of surgical specialties, including plastic and reconstructive surgery, neurosurgery, gastrointestinal and related organ surgery, urological surgery, general surgery, orthopedic surgery, gynecological surgery, thoracic surgery, and laparoscopic surgery, where aspiration of adipose tissue is desired.

[0017] (Liposuction tissue washing system) FIG. 1 shows an exploded view of the disclosed liposuction tissue cleaning system 100. As shown in FIG. 1, the system 100 can include a chamber 130 having a hollow body formed with a sidewall having an outer surface and an inner surface, the chamber being open at the top and bottom of the chamber. The upper opening of the chamber 130 can be used to add liposuction tissue to the system 100 so that the liposuction tissue can be cleaned. The disclosed system 130 advantageously uses a single chamber 130 instead of multiple chambers used by known systems. In some embodiments, the chamber 130 can be graduated to allow a user to determine the volume of material inside the chamber 130, as shown in FIG. 1. The chamber 130 can be made from any material, including polymers (e.g., high-density polyethylene, polypropylene), metals, and combinations thereof. The chamber 130 can be configured to house or support the remaining components of the system 100, such as the lid 108 and tray 140.

[0018] The chamber 130 may include an extraction port 132 to allow the washed and / or concentrated liposuction tissue to be removed from the system 100. The washed and concentrated liposuction tissue can be extracted from the system 100 through the extraction port 132. The extraction port 132 may include a cylindrical hole in the lower portion of the chamber 130 that protrudes outward from the side of the chamber 130. The extraction port 132 may connect to a hose, syringe, or other extraction device to facilitate extraction of the washed and concentrated liposuction tissue from the system 100. The extraction port 132 may include threads configured to connect to an extraction port cap 136 and an extraction port cosmetic cap 138, which close the extraction port 132 when not being used to remove washed liposuction tissue from the system 100. The extraction port 132 may include an extraction port gasket 134 that provides a seal between the extraction port 132 and the extraction port cap 136 and / or extraction port cosmetic cap 138. The seal provided by the extraction port gasket 134 may prevent accidental leakage from the system 100. In some embodiments, the chamber 130 may include multiple extraction ports 132. Although not specifically shown in FIG. 1, there are waste drain ports that may be used to extract waste liquid from the system.

[0019] The disclosed system 100 has various configurations, such as an open, closed, or semi-closed configuration. An open configuration is a configuration in which the lid 108 is not attached to the chamber 130 of the system 100. The lid 108 may be coupled to the top of the chamber 130, thereby providing a closed or semi-closed top cover for the system 100. The lid 108 may include a top surface and a bottom surface. The lid 108 may include a lip extending downward from the bottom surface of the lid and configured to securely connect the lid to the top side of the chamber 130. The lid 108 may include a harvest port 106 on the top surface of the lid 108. The harvest port 106 may include a cylindrical tap extending outward from the top side of the lid 108 and may be configured to connect to a hose or other source to receive harvested lipoaspirate tissue from the hose. Harvested lipoaspirate tissue includes any lipoaspirate tissue obtained from a human or animal source, such as via liposuction. Harvesting of liposuction tissue can be accomplished by known methods, such as liposuction or manual harvesting. The harvest port 106 may be threaded or smooth to mate with various types of hoses or device direct couplings. The harvest port 106 may be recessed from the top surface of the lid 108, flush with the top surface of the lid 108, or protrude from the top surface of the lid 108. FIG. 1 illustrates a recessed harvest port 106 configuration. In some embodiments, the lid 108 may include multiple harvest ports 106, such as having 1 to 5 harvest ports 106 or more.

[0020] 1 , the disclosed system 100 may include a lid 108 having an opening that may provide a user with access to the contents of the system 100. For example, the opening in the lid 108 may allow a user to add fluids (e.g., irrigation fluids) and harvested liposuction tissue to the system 100. During the irrigation process, a user may access the contents of the system 100 through the lid 108, such as by using a spatula 112 to mix the contents. The opening in the lid 108 may advantageously provide for the system 100 to be semi-closed or closed, depending on the user's preference.

[0021] In its semi-closed configuration, the system 100 incorporates a lid 108 having a small, removable port cap 102. The port cap 102 can advantageously be used to add fluids and other substances, such as a mixture of irrigation packets and saline, to the lipoaspirate tissue, and to use a tool such as a spatula 112 to mix and irrigate the harvested lipoaspirate tissue with a fluid or a mixture of P188 and saline. The lid 108 can include a port gasket 104 that provides a seal between the port cap 102 and the lid 108. In some embodiments, the lid 108 can be configured with two or more openings, and thus two or more port caps 102. For example, the lid 108 can be configured with one or more port caps 102, such as one to five port caps 102 or more. In the closed configuration, the system 100 can have a lid without a port cap 102 or can be operated without removing the port cap 102.

[0022] As shown in FIG. 1 , the system 100 may include a spatula 112 and one or more irrigation packets 114. Each irrigation packet may be mixed with a fluid (e.g., saline) to prepare a irrigation solution for irrigating the harvested liposuction tissue. The irrigation packets 114 may include any surfactant known in the art for irrigating liposuction tissue. For example, the irrigation packet 114 may include a biocompatible block copolymer, such as a poloxamer surfactant having repeating units of polyethylene oxide (PEO) and polypropylene oxide (PPO). The disclosed system 100 may include a irrigation packet 114 containing a composition such as that disclosed in U.S. Pat. No. 8,512,695. The polymeric surfactant preferably includes P188. The irrigation packet 114 may include saline components, including any known salts, such as sodium chloride, potassium chloride, and other salts. The spatula 112 may be made of any known material, including polymers (e.g., polyethylene, polypropylene) and metals.

[0023] As shown in FIG. 1 , the system 100 for cleaning liposuction tissue may include a subassembly 116 including a mesh basket with an opening at its top. The subassembly 116 may be configured to seat within the upper portion of the chamber 130. In some embodiments, the subassembly may receive collected liposuction tissue through the opening at its top and contain the collected liposuction tissue along with the mesh basket of the subassembly during any cleaning or processing steps. The mesh basket allows waste fluid to separate from the liposuction tissue as it is cleaned. The subassembly 116 may receive collected liposuction tissue through the collection port 106 of the lid 108. The mesh basket of the subassembly 116 may include a mesh bottom 126 forming the bottom of the subassembly. The mesh basket may have multiple mesh sides 118 forming the sides of the subassembly. The mesh size of the mesh basket may include mesh ranging from about 2 mesh (e.g., 11,100 microns) to about 400 mesh (e.g., 37 microns). For example, the mesh size may be about 2 mesh, about 2 mesh, about 50 mesh, about 100 mesh, about 150 mesh, about 200 mesh, about 250 mesh, about 300 mesh, about 350 mesh, or about 400 mesh, where about includes plus or minus 25 mesh.

[0024] According to some embodiments, as shown in FIG. 1 , the disclosed subassembly may include a circular hole 120 at the bottom of the subassembly. As shown in FIG. 1 , the circular hole 120 may be seated in alignment with the extraction port 132 of the chamber 130. The circular hole 120 may also be seated above the extraction port 132. In some embodiments, the circular hole 120 may be fitted with a slit valve 122 and a slit lock 124, which may form a seal or partial seal with the extraction port 132, allowing the washed and concentrated lipoaspirate tissue to be extracted from the subassembly and out of the chamber 130 through the extraction port 132.

[0025] As shown in FIG. 1 , the disclosed liposuction tissue cleaning system 100 may include a tray 140 configured to be inserted into the lower portion of the chamber 130 through an opening in the sidewall, thereby forming the bottom of the system 100. As the liposuction tissue is cleaned, cleaning fluid drips from the liposuction tissue contained within the subassembly into the tray 140. The tray 140 may include a plate having a generally flat shape with a raised sidewall disposed along the entire periphery of the plate and extending upward from the upper surface of the plate. The upper surface of the plate may have various textures, such as those shown in FIG. 1A . The various textures may facilitate fluid drainage. The tray 140 may include a foam 146 held in place by the container elements 142 and 144. The foam 146 may be seated within the sidewalls of the tray 140 and within the container elements 142 and 144. In some embodiments, the foam 146 may have a thickness ranging from about 0.5 inches to about 3 inches. The foam 146 may have a thickness of about 0.5 inches, about 1 inch, about 1.5 inches, about 2 inches, about 2.5 inches, or about 3 inches, where about includes plus or minus 0.25 inches. The foam 146 has a thickness of about 25 g / 100 cm 2 (3mm thickness) ~ approx. 75g / 100cm 2 The foam 146 may have an absorbent capacity in the range of about 25 g / 100 cm at a foam thickness of 3 mm. 2 , about 30g / 100cm 2 , about 35g / 100cm 2 , about 40g / 100cm 2 , 45g / 100cm 2 , about 50g / 100cm 2 , about 55g / 100cm 2 , about 60g / 100cm 2 , about 65g / 100cm 2 , about 70g / 100cm 2 , or approximately 75g / 100cm 2 where approximately is plus or minus 2.5 g / 100 cm 2 In some embodiments, the foam 146 has a thickness of 3 mm and a weight of about 47.9 g / 100 cm 2The foam 146 may have an absorbent capacity of about 100 cm 2 ~about 500cm 2 For example, the foam 146 may have an area in the range of about 100 cm 2 , about 150cm 2 , about 200cm 2 , about 250cm 2 , about 300cm 2 , about 350cm 2 , about 400cm 2 , about 450cm 2 , or approximately 500 cm 2 In some embodiments, the foam 146 may have an area of ​​182 cm 2 When the system 100 is assembled, the foam 146 may be positioned within the lower portion of the chamber 130, directly below the subassembly 116. During the cleaning process, the cleaned lipoaspirate tissue may come into contact with the foam 146, where it thickens. The disclosed foam 146 advantageously thickens the cleaned lipoaspirate tissue by absorbing such waste and cleaning fluids.

[0026] The disclosed foam 146 may include multiple foam members, forming a complex channel system configured to redistribute fluid from the liposuction tissue throughout the foam, thereby concentrating the liposuction tissue. In some embodiments, the disclosed foam 146 advantageously prevents the foam from becoming saturated as it absorbs fluid from the liposuction tissue, thereby concentrating the liposuction tissue. In some embodiments, the tray 140 may include multiple adhesive-backed bumpers 148, 150 that prevent the tray 140 from sliding and absorb shock to the tray 140 and the system 100. The bumpers 148, 150 prevent the system 100 from moving around, thus keeping the system 100 stable on a surface such as a table or floor. In some embodiments, the bumpers 148, 150 may have different heights to provide a slight slope to the system 100 to direct fluids (e.g., waste fluid and washed liposuction tissue) to various portions of the system 100. The adhesive-backed bumpers 148, 150 may be in a variety of shapes and sizes, including having an outer diameter of about 1 / 8 inch, about 1 / 4 inch, about 3 / 8 inch, about 1 / 2 inch, about 5 / 8 inch, about 3 / 4 inch, where about includes plus or minus 1 / 16 inch. The adhesive-backed bumpers 148, 150 may have a height of about 1 / 4 inch, about 3 / 8 inch, about 1 / 2 inch, about 3 / 4 inch, or about 1 inch, where about includes plus or minus 1 / 8 inch.

[0027] A user may desire to precisely control when the cleaned liposuction tissue leaves the subassembly 116 (top portion) of the system 100 and falls into the tray (bottom portion) of the system 100. By removing the removable separator 154 from the system 100, the cleaned liposuction tissue is collected in the tray 140, thereby contacting the foam 146. The removable separator 154 may be disposed on an upper surface of the raised sidewall of the tray 140 and includes a gasket 152 configured to form a seal between the removable separator 154 and either the chamber 130 or the tray 140. The removable separator 154 may include a handle 156 that protrudes outward from a side of the removable separator 154, and thus outward from the chamber 130, and that is configured to be pulled to remove the removable separator 154 from the chamber 130. The system 100 may include a squeegee 128 configured to prevent fluid from leaking from the system 100 while the removable separator 154 is removed from the system 100. The removable separator 154 may include any number of gaskets to help seal spaces between the removable separator 154 and other components of the system 100 while the removable separator 154 is coupled to the system. The gaskets may provide air and fluid sealing functions.

[0028] Component numbers increase incrementally based on which figure they refer to. For example, component 100 in FIG. 1 is annotated as component 200 in FIG. 2 and as component 300 in FIG. 3. FIGS. 2A and 2B show top and isometric views of liposuction tissue washing system 200 with the components shown in FIG. 1 assembled together. As shown in FIGS. 2A and 2B, lid 208 may be attached to chamber 230. FIGS. 2A and 2B show an embodiment in which port cap 202 is inserted into lid 208. Similarly, as shown in FIGS. 3A-3C, a user may remove either port cap 302 or lid 308 to gain various types of access to the interior of liposuction tissue washing system 300. The ability to remove either the port cap 302 or the lid 308 gives the user more flexibility in using the system (e.g., to add a saline and P188 mixture to harvested lipoaspirate tissue and to mix the P188 and saline mixture with harvested fat using a mixing device such as the spatula 112), but also reduces the surface area directly exposed to air. In terms of surface area, direct exposure to air from above is reduced compared to prior art systems (FIG. 3B) and can be quickly reduced to 0% by placing the port cap 302 in the opening while the user is not mixing or cleaning (FIG. 3A). In the open system modality (FIG. 3C), the user has complete and unobstructed access to the lipoaspirate tissue by simply removing the lid 308 covering the system 300.

[0029] 4A and 4B show a foam configuration with channels along the periphery. The system 400 may include a highly absorbent foam 446 disposed within a tray 440 and held in place by container elements 442 and 444. The foam 446 may initially be separated from the chamber's mesh basket by a removable separator adapted to be removed from the chamber by simply pulling the removable separator out, allowing the mesh basket to contact the foam 446. The foam 446 has an advanced foam geometry for maximizing fluid absorption and producing highly concentrated fat. After washing the harvested lipoaspirate tissue, the washed lipoaspirate tissue may be concentrated by placing it on the foam 446 contained within the tray 440. During the concentration step (using the foam) of the washing method, a single chamber device may be capable of handling large amounts of fluid and oil that may remain after separating waste liquid from the washed lipoaspirate tissue, while simultaneously concentrating the remaining lipoaspirate tissue as quickly as possible. To control this large volume of oil and fluid, foam 446 may include channels running along its entire perimeter, which not only increase the surface area of ​​the foam-fluid interface but also act as a reservoir for the large, rapid volumetric evacuation at the beginning of the condensation process. The added surface area allows the foam of foam 446 to rapidly expand and contact the overlying mesh. As shown in Figures 4A and 4B, this contact process between the foam and the mesh ensures condensation of the liposuction tissue.

[0030] 5, foam 546 held in place by container members 542 and 544 can include a complex channel system 558 for redistributing fluid throughout foam 546. This allows for at least two advantages. First, channel system 558 ensures that the absorption capacity of foam 546 is maximized by expanding the surface area of ​​the foam-fluid interface. Second, it allows for excess fluid to pool in the foam 546 area of ​​the tray, preventing the foam from becoming saturated before it can concentrate the liposuction tissue.

[0031] As shown in FIGS. 6A-6C, the disclosed system 600 may include a removable separator 654 having a handle 656. After cleaning the harvested lipoaspirate tissue, the removable separator 654 may be removed, allowing the cleaned lipoaspirate tissue to contact the foam. The foam condenses the cleaned lipoaspirate tissue, forming concentrated lipoaspirate tissue. FIG. 6 shows side, top, and isometric views of the removable separator 654. The removable separator 654 facilitates processing the lipoaspirate tissue for reinjection into the patient using only a single chamber 130 (as shown in FIG. 1) to accommodate both the cleaning and condensing steps. The removable separator 654 ensures that the lipoaspirate tissue is properly cleaned and drained before being exposed to the condensing foam. The removable separator 654 also advantageously facilitates both an airtight and fluid seal, is able to support a vacuum without significant deflection, does so within a tightly constrained space, and facilitates easy removal by the user.

[0032] In some embodiments, the system may also include a self-cleaning feature for cleaning the removable separator when it is removed from the system. As shown in FIG. 7 , the system may include a squeegee 728 as a self-cleaning feature. The squeegee 728 may be an L- or U-shaped bracket with one side bent into a quarter circle, followed by a straight length, and then bent into a second quarter circle. The squeegee 728 is configured to seat within a portion of the chamber so that it is held in place, with a portion of its body hanging just above the top surfaces of the removable separator and foam. Removing the removable separator from the chamber causes the removable separator to slide against the bottom surface of the squeegee 728, thereby removing waste from both top surfaces of the removable separator. The squeegee 728 and the removable separator prevent liposuction tissue and waste from escaping the system and direct the cleaned liposuction tissue into the foam. In some embodiments, removal of the removable separator allows for cleaning of the removable separator via a squeegee-like action from squeegee 728. The removable separator coupled with squeegee 728 can help prevent leaks in the system in the event of an overflow.

[0033] 8 shows a top view of the disclosed subassembly 816. The disclosed system can include a fluid drainage management system through various components and features of the design of the disclosed subassembly 816. As shown in FIG. 8, the subassembly 816 can have a 45-degree cutout feature (other angles are contemplated) on the back of the subassembly. The subassembly 816 can also have a slight ridge on the back of its frame to increase flow to the drain.

[0034] 9A and 9B show side and top views of the chamber 930. The chamber 930 may include a waste fluid drain port 974 configured to allow waste fluid separated from the liposuction tissue during the cleaning process to be removed from the system. The waste fluid drain port 974 may be located opposite the extraction port 932 and may be located at a lower height compared to the extraction port 932. For example, the waste fluid drain port 974 may be further from the lid than the extraction port 932. The waste fluid drain port 974 may include any type of known closure mechanism for initiating and terminating the drainage of waste fluid from the system. In some embodiments, suction, such as by vacuum, may be applied to the waste fluid drain port 974, which may facilitate removal of waste fluid from the system.

[0035] In some embodiments, as shown in FIG. 10 , the chamber 1030 of the system 1000 may be slightly sloped toward the waste drain port 1074, which may advantageously allow for better collection of waste fluid from the system 1000. FIG. 10 also shows an extraction port 1074 on the opposite side of the system 1000 from the waste drain port 1074. As shown in FIG. 10 , the waste drain port 1074 may be located lower than the extraction port 1032. In some embodiments, the slope may involve tilting the system and / or tilting the basket. In some embodiments, the system may be tilted toward the waste drain port 1074. To aid in the extraction of the cleaned liposuction tissue, the basket and / or subassembly may be tilted toward the extraction port 1074.

[0036] According to some embodiments, the disclosed system 1100 for cleansing liposuction tissue can be housed in a container for storage or transportation, as shown in FIG. 11 . For example, the system 1100 can be placed on a sheet 1162, which can then be placed within an envelope 1160. Both the sheet 1162 and the envelope 1160 can be made of a variety of materials, including paper, plastic, metal, and mixtures thereof. As shown in FIG. 12 , the system 1200 can be placed within an inner pouch 1284. A pouch handle 1294 can be attached from one end of the inner pouch 1284 to the other end of the inner pouch 1284, thereby securing the system 1200 inside. The pouch handle 1294 can allow a user to carry the system 1200 contained inside the inner pouch 1284 without touching the outside of the inner pouch 1284. The secured system 1200 and inner pouch 1284 can be added together to an outer bag 1286. Each of the inner bag 1284 and outer bag 1286 may be sterile. The inner bag 1284 and outer bag 1286 containing the system 1200 may be inserted into a carton 1288 containing a foam insert 1290. Once secured inside the carton 1288, a foam top layer 1292 may be placed on top to provide top protection for the system 1200. A device identification label 1296 may be placed on the exterior of the carton 1288 for identification and tracking purposes.

[0037] 13A-13B show various views of the disclosed system 1300 for cleansing liposuction tissue. As shown in FIG. 13, the system 1300 has graduations along the side, allowing a user to determine the volume of contents contained within the system 1300. FIGS. 14A-14C show various views of the disclosed chamber 1480 without the lid, also showing the graduation markings. In some embodiments, as shown in FIGS. 14A-14C, the disclosed chamber 1480 can include a reinforced outer wall, as shown in FIG. 14C. The reinforced outer wall of the chamber 1480 advantageously prevents cracking when the system is subjected to sustained liposuction pressure.

[0038] 15A-15C show various views of the disclosed foam 1546 held in place by container elements 1542 and 1544. The foam may be contained by outer element 1544 and inner element 1542.

[0039] FIGS. 16A-16F show various views of a port cap 1602 for insertion into the lid of a system for cleaning liposuction tissue. The port cap 1602 may include a substantially flat base and a handle protruding outward from the top surface of the base. The bottom surface of the base of the port cap 1602 may be configured to fit onto the top surface of the lid. In some embodiments, the port cap 1602 may be configured with a port gasket to enhance the seal between the port cap 1602 and the lid. FIGS. 17A-18C show various views of a lid 1708 configured to hold the port cap 1602 shown in FIGS. 16A-16F. FIGS. 17A and 17B show the top of the collection port 1706 of the lid 1708. As shown in FIGS. 17E-17F, the lid 1708 may include a plurality of ribs 1790 extending from the port to the interior surface of one of the lid's side walls. The ribs 1790 protect the lid from cracking while under pressure. The ribs 1790 may allow the lid to maintain suction pressure.

[0040] FIGS. 19A-20E show various views of the graduated chamber 1930, 2030. The chamber 1930, 2030 may include graduated markings on one or two sides. In some embodiments, the chamber 1930, 2030 may include threads around the extraction port 1932 for coupling to a syringe. The extraction port may include a gasket that provides a seal to prevent pressure loss when drawing aspirate through a syringe. As shown in FIGS. 19A-19H, the subassembly 1916 may include vertical ridges along the interior surface of the chamber 1916. For example, the subassembly 1916 may include one to ten ridges. FIGS. 19A-20E show the chamber 1930, 2030 not coupled to a removable separator. In contrast, FIGS. 21A-21C show the chamber 2130 coupled to a tray 2140. 22A-22F show various views of the subassembly 2216.

[0041] In some embodiments, as shown in FIGS. 23A-23J, the tray 2340 can include channels 2366 that can help separate waste fluid from the cleaned liposuction tissue. The disclosed channels 2366 can be raised or recessed relative to the tray. In some embodiments, the disclosed channels 2366 can be a combination of raised and recessed channels, as shown in FIG. 23F. FIGS. 23G-23H show a tray 2340 with protrusions 2368 along the sides of the tray 2340. The protrusions 2368 can facilitate engagement when the tray 2340 is mated with a chamber. The protrusions 2368 can advantageously align portions of the tray 2340 and the chamber when they are mated.

[0042] As shown in FIGS. 24A-24G, the disclosed system for cleansing liposuction tissue can include a removable separator 2454. The removable separator can include a substantially flat shape, such as a rectangular parallelepiped. As shown in FIGS. 24A, 24E, and 24F, the removable separator 2454 can include a series of channels on the bottom side of the removable separator 2454, which can advantageously help bind the contents of the tray when the removable separator 2454 is fitted inside the chamber. As shown in FIGS. 24C-24F, the removable separator 2454 can include a handle 2456 that can protrude outward from the chamber. The handle 2456 can be grasped by a user to facilitate pulling the removable separator 2454 out of the chamber. The handle 2456 may be any common shape such as a circle, square, triangle, polygon, cylinder, cube, rectangular prism, cone, pyramid, prism, and combinations thereof. The handle 2456 may be a solid piece or may include a hole therethrough, such as a circular hole, as shown in Figures 24C-24F. Having a hole through the handle 2456 allows for easier grasping when placing the removable separator 2454 in or out of the chamber.

[0043] (Method for washing harvested liposuction tissue) The present disclosure relates to a method for cleaning harvested lipoaspirate tissue using the disclosed system as shown in Figures 1-24. The disclosed method involves a combination of cleaning (e.g., using a cleaning solution such as a mixture of P188 and saline, or a mixture of saline and another type of surfactant), mesh filtering, concentrating the lipoaspirate tissue, and absorbing and removing waste products from the cleaning system, leaving behind cleaned lipoaspirate tissue. The cleaning solution can include a mixture of P188 and saline, or a mixture of saline and another surfactant. The cleaned lipoaspirate tissue can be transplanted into a patient.

[0044] In some embodiments, the present disclosure relates to a method for cleaning harvested lipoaspirate tissue using a liposuction tissue cleaning system. The method includes receiving harvested lipoaspirate tissue into a chamber of the liposuction tissue cleaning system such that the harvested lipoaspirate tissue is contained by a mesh basket disposed within an upper portion of the chamber of the liposuction tissue cleaning system, thereby forming received lipoaspirate tissue. The harvested lipoaspirate tissue may be harvested from various donor sites on a patient. The method may include attaching a harvesting port to a liposuction cannula.

[0045] Once the harvested lipoaspirate tissue is received in the chamber, the method may include adding a lavage fluid, such as, but not limited to, that described in U.S. Patent No. 8,512,695, to the chamber and mixing the lavage fluid with the received lipoaspirate tissue to form a mixed lipoaspirate tissue. Mixing the lavage fluid with the received lipoaspirate tissue can extract waste products from viable adipose tissue. The lavage fluid may include a biocompatible block copolymer, such as a poloxamer surfactant having repeating units of polyethylene oxide (PEO) and polypropylene oxide (PPO). In some embodiments, the copolymer is poloxamer P188. The lavage fluid may include a saline solution containing water combined with any known salt, such as sodium chloride, potassium chloride, and other salts. In some embodiments, mixing the lavage fluid with the received lipoaspirate tissue can be performed using a spatula for a period ranging from about 1 minute to about 10 minutes, or longer. For example, mixing may occur for a period of about 1 minute, about 2 minutes, about 3 minutes, about 4 minutes, about 5 minutes, about 6 minutes, about 7 minutes, about 8 minutes, 9 minutes, or about 10 minutes, where about includes plus or minus 0.5 minutes.

[0046] According to some embodiments, the washing solution can be added to the chamber in various concentrations relative to the lipoaspirate tissue to form a mixed lipoaspirate tissue. The washing solution can be added to the chamber so that the ratio of washing solution to the received lipoaspirate tissue ranges from about 1:4 to about 4:1. For example, the ratio of washing solution to the received lipoaspirate tissue can be about 1:4, about 1:3, about 1:2, about 1:1, about 2:1, about 3:1, or about 4:1, where about includes plus or minus 0.5:0.5.

[0047] In some embodiments, adding the washing fluid to the chamber containing the harvested liposuction tissue can include pouring the washing fluid through an opening in a lid firmly connected to the top of the chamber of the liposuction tissue washing system. Adding the washing fluid can include pouring the washing fluid directly into the chamber without the lid being present. The lid can also include a port cap that can be pulled away from the lid to provide the opening. Replacing the port cap can seal the lid. In addition to pouring, the washing fluid can be injected or pumped using any known fluid transport means.

[0048] After mixing the irrigation fluid with the received lipoaspirate tissue to form a mixed lipoaspirate tissue, the method may include separating the mixed lipoaspirate tissue into an extracted waste fluid and a washed lipoaspirate tissue. The extracted waste fluid may contain blood, oil, water, and other undesirable waste products that may reduce the success rate of the washed lipoaspirate tissue when used for fat grafting.

[0049] In some embodiments, the washed lipoaspirate tissue and / or concentrated lipoaspirate tissue can be removed from the lipoaspirate tissue washing system through an extraction port located within the lower portion of the chamber sidewall. The concentrated lipoaspirate tissue can have a higher lipoaspirate tissue concentration per volume compared to the harvested lipoaspirate tissue. The concentrated lipoaspirate tissue can have a lipoaspirate tissue concentration per unit volume that is about 2 to about 100 times higher than the harvested lipoaspirate tissue. The concentrated lipoaspirate tissue can have a lipoaspirate tissue concentration per unit volume that is 2-fold higher, about 10-fold higher, about 20-fold higher, about 30-fold higher, about 40-fold higher, about 50-fold higher, about 60-fold higher, about 70-fold higher, about 80-fold higher, about 90-fold higher, or about 100-fold higher than the harvested lipoaspirate tissue, where about includes plus or minus 5-fold.

[0050] According to some embodiments, the removable separator can separate the mixed liposuction tissue contained in the mesh basket located at the top of the chamber from the tray and extraction port, both located at the bottom of the chamber. The method can include removing the removable separator from the liposuction tissue washing system so that the washed aspirated tissue can contact the foam. A user can pull a handle protruding from the side of the removable separator, thereby removing the removable separator from the side of the system. A portion of the extracted waste material can be absorbed into a foam assembly contained in the tray, forming absorbed waste that can be discarded separately from the liposuction tissue washing system. Absorption of the extracted waste material into the foam assembly can concentrate the washed aspirated tissue, forming concentrated liposuction tissue. The concentrated liposuction tissue can then be removed from the washing system through an extraction port located in a lower portion of the side wall of the chamber.

[0051] According to some embodiments, the disclosed method for washing lipoaspirate tissue can be iterative in that it can be performed multiple times in a batch or flow. For example, the method can be performed once, resulting in the formation of washed lipoaspirate tissue, and the extracted waste fluid can be removed from the disclosed system, after which additional harvested lipoaspirate tissue can be added to the chamber for washing. This can be used to increase the yield of washed lipoaspirate tissue. The disclosed method can include receiving additional harvested lipoaspirate tissue into the chamber of the lipoaspirate tissue washing system, such that the additional harvested lipoaspirate tissue is contained by a mesh basket positioned within the upper portion of the chamber of the lipoaspirate tissue washing system to form the additional received lipoaspirate tissue. In a batch, the disclosed system and method can be capable of washing from approximately 10 mL of harvested lipoaspirate tissue to approximately 2,000 mL of harvested lipoaspirate tissue. For example, the disclosed systems and methods may wash about 10 mL, about 200 mL, about 400 mL, about 600 mL, about 800 mL, about 1,000 mL, about 1,200 mL, about 1,400 mL, about 1,600 mL, about 1,800 mL, or about 2,000 mL of harvested lipoaspirate tissue, where about includes plus or minus 100 mL. In one embodiment, the disclosed systems or methods may wash about 1,050 mL of harvested lipoaspirate tissue. Alternatively, washed lipoaspirate tissue may be continuously removed from the disclosed systems as new batches of harvested lipoaspirate tissue are added, so that the system operates in a flow mode rather than a batch mode.

[0052] The disclosed methods may include adding additional irrigation fluid to the chamber and mixing the irrigation fluid with the lipoaspirate tissue with a spatula or other mixing device to form an additional amount of mixed lipoaspirate tissue. In some embodiments, the disclosed methods may include separating additional effluent fluid from the additional mixed lipoaspirate tissue to form an additional amount of extracted effluent fluid and an additional amount of washed lipoaspirate tissue, the extracted effluent fluid being removed from the lipoaspirate tissue washing system through an extraction port located in the lower portion of the sidewall of the chamber.

[0053] The terms used in the description herein are for the purpose of describing particular embodiments and are not intended to be limiting. As used in the description of the invention and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly dictates otherwise. It will also be understood that the term "and / or," as used herein, refers to and encompasses any possible combination of one or more of the associated listed items. It will be further understood that the terms "includes," "including," "comprises," and / or "comprising," as used herein, specify the presence of stated features, steps, operations, elements, and / or components, but do not exclude the presence or addition of one or more other features, steps, operations, elements, components, and / or groups thereof.

[0054] As used herein, the term "if" may be interpreted to mean "when" or "upon" or "in response to determining" or "in response to detecting," depending on the context.

[0055] Those skilled in the art will understand that while some terms in this disclosure may refer to absolute terms, the methods and techniques disclosed herein may be performed on less than all given components, such as on one or more components. Thus, when absolute terms are used in this disclosure, the disclosure may also be interpreted as referring to a subset.

Claims

1. 1. A system for irrigating liposuction tissue, comprising: (a) a chamber comprising a hollow body formed with a sidewall having an outer surface and an inner surface, the chamber being open at a top and a bottom; (b) a lid, (i) a top surface; (ii) a lip extending downwardly from a bottom surface of the lid, the lip configured to securely connect the lid to the upper side of the chamber; (iii) a harvesting port including a cylindrical tap extending outward from the top side of the lid, the harvesting port configured to connect to a hose and receive harvested lipoaspirate tissue from the hose; a lid comprising: (c) a subassembly including a mesh basket disposed within an upper portion of the chamber, the subassembly configured to receive the lipoaspirate tissue received through the collection port and to separate effluent extracted from the lipoaspirate tissue to form washed lipoaspirate tissue; (d) a tray configured to be inserted into the lower portion of the chamber through the opening in the side wall, thereby forming the bottom of the system; (i) a plate; (ii) a raised sidewall disposed along the entire periphery of the plate and extending upwardly from the upper surface of the plate; (ii) a foam assembly fitted over the plate and within the sidewall to be positioned within the lower portion of the chamber, the foam assembly being configured to concentrate the cleansed lipoaspirate tissue; a tray comprising: (e) an extraction port along one of the side walls of the chamber configured to allow removal of one of washed lipoaspirate tissue and concentrated lipoaspirate tissue; A system for cleaning liposuction tissue, comprising:

2. the extraction port comprises a cylindrical hole in the lower portion of the chamber protruding outward from a side of the chamber; and the extraction port comprising threads configured to couple to an extraction port cap that contains the extract within the system; At least one of The system for irrigating liposuction tissue according to claim 1 .

3. The system for cleaning liposuction tissue of claim 1 , wherein the lid further comprises a compartment for storing at least one of a spatula and a cleaning packet.

4. the system further comprising a removable separator disposed on an upper surface of the raised sidewall of the tray, the removable separator configured to separate contents contained within the chamber from the tray; The removable separator a gasket configured to form a seal between the removable separator and the chamber; a handle projecting outward from a side of the removable separator, and thus outward from the chamber, the handle being configured to be pulled to remove the removable separator from the chamber; Equipped with The system for irrigating liposuction tissue according to claim 1 .

5. the foam assembly comprises a plurality of foam members forming a complex channel system configured to redistribute fluid from the liposuction tissue throughout the foam assembly; the foam assembly is configured to prevent the foam member from becoming saturated before the foam member can concentrate the liposuction tissue. The system for irrigating liposuction tissue according to claim 1 .

6. The system for cleaning liposuction tissue of claim 1 , wherein the lid includes an opening on an upper surface of the lid configured to allow access to an interior of the mesh basket.

7. 2. The system for irrigating liposuction tissue of claim 1, wherein the mesh basket comprises a mesh bottom forming the bottom of the subassembly and a plurality of mesh sides forming the sides of the subassembly.

8. The system for cleaning liposuction tissue of claim 1 , wherein the tray further comprises a squeegee.

9. 10. The system for cleaning liposuction tissue of claim 1, wherein the lid further comprises a port cap that fits into an opening on the top surface of the lid and is configured to be removed from the opening to allow a user to access the interior of the chamber with a spatula.

10. the subassembly having a circular hole in a bottom portion thereof that aligns with an extraction port of the chamber; the circular hole is configured to form a seal with the extraction port via a slit valve; The system for irrigating liposuction tissue according to claim 1 .

11. 1. A method for cleaning harvested liposuction tissue using a liposuction tissue cleaning system, comprising: (a) receiving the harvested lipoaspirate tissue into a chamber of the lipoaspirate tissue washing system such that the harvested lipoaspirate tissue is contained by a mesh basket disposed within an upper portion of the chamber of the lipoaspirate tissue washing system to form received lipoaspirate tissue; (b) adding a wash fluid to the chamber and mixing the wash fluid with the received lipoaspirate tissue using a spatula to form a mixed lipoaspirate tissue; (c) separating the mixed lipoaspirate tissue into extracted waste liquid and washed lipoaspirate tissue; 1. A method for cleaning liposuction tissue, comprising:

12. (d) removing a removable separator from the liposuction tissue washing system so that the washed aspirated tissue may descend onto a foam assembly contained within a tray contained within a lower portion of the chamber, the tray being positioned below the mesh basket; (e) absorbing a portion of the extracted effluent into the foam assembly to concentrate the washed aspirated tissue to form concentrated lipoaspirate tissue; Further comprising: and removing one of the washed lipoaspirate tissue and the concentrated lipoaspirate tissue from the lipoaspirate tissue washing system through an extraction port located in a lower portion of a sidewall of the chamber.

12. The method for irrigating liposuction tissue according to claim 11.

13. the enriched lipoaspirate tissue comprises a lipoaspirate tissue concentration per unit volume that is about 2 to about 100 times higher than the harvested lipoaspirate tissue; 12. The method for irrigating liposuction tissue according to claim 11.

14. 12. The method for irrigating liposuction tissue of claim 11, further comprising attaching the harvesting port to a liposuction cannula.

15. adding the cleaning solution to the chamber includes pouring the cleaning solution through an opening in the lid that tightly connects to an upper side of the chamber via a lip extending downwardly from a bottom surface of the lid; 12. The method for irrigating liposuction tissue according to claim 11.

16. adding a washing solution to the chamber at a ratio of the washing solution to the received lipoaspirate tissue in a range of about 1:4 to about 4:1; and The mixed liposuction tissue is allowed to stand for a period of time ranging from about 30 seconds to about 5 minutes; 12. The method for irrigating liposuction tissue according to claim 11, wherein the method comprises at least one of:

17. said mixing said irrigation solution with said received liposuction tissue with a spatula is performed for a time period ranging from about 1 minute to about 10 minutes; and the cleaning solution comprises a biocompatible block copolymer comprising repeating units of polyethylene oxide (PEO) and polypropylene oxide (PPO); 12. The method for irrigating liposuction tissue according to claim 11, wherein the method comprises at least one of:

18. (d) receiving additional harvested lipoaspirate tissue into the chamber of the lipoaspirate tissue washing system such that the additional harvested lipoaspirate tissue is contained by the mesh basket positioned within the upper portion of the chamber of the lipoaspirate tissue washing system to form additional received lipoaspirate tissue; 12. The method for irrigating liposuction tissue of claim 11, further comprising:

19. (e) adding additional washing fluid to the chamber and mixing the washing fluid with the received lipoaspirate tissue with the spatula to form additional mixed lipoaspirate tissue; 20. The method for irrigating liposuction tissue of claim 18, further comprising:

20. (f) separating additional effluent from the additional mixed liposuction tissue to form additional extracted effluent and additional washed liposuction tissue, wherein the extracted effluent is removed from the liposuction tissue washing system through an extraction port located in a lower portion of a sidewall of the chamber.

20. The method for irrigating liposuction tissue according to claim 19.

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