Fermentation apparatus for high-yield nattokinase strains

Abstract

This invention provides a fermentation apparatus for high-yielding nattokinase strains. [Solution] The system includes a fermentation tank 1, a support frame 2 fitted to the outside of the fermentation tank, a bottom plate 3 fixedly connected to the lower end of the support frame, a material supply pipe fixedly connected to the upper end of the fermentation tank, an aeration pipe 6 fixedly connected to the lower end of the fermentation tank, and an aeration assembly rotatably connected to one end of the aeration pipe. By coordinating the rotating cavity 7 of the aeration assembly with a plurality of branched aeration members 8, sterile gas is converted into fine bubbles by passing it through the fine pores of the microbubble aeration head, thereby significantly increasing the gas-liquid contact area. At the same time, the reaction force generated during aeration drives the rotating cavity and branched aeration members to rotate synchronously, so that the fine bubbles diffuse uniformly into each region of the internal cavity of the fermentation tank.

Description

Technical Field

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[0001] The present invention relates to the field of microbial fermentation equipment, and particularly to a fermentation device for high-yield nattokinase strains.

Background Art

[0002] Nattokinase is a serine protease isolated from natto, has strong fibrinolytic activity, and has broad application prospects in the field of prevention and adjuvant treatment of cardiovascular and cerebrovascular diseases. Currently, nattokinase is mainly produced industrially by microbial fermentation method. Here, the fermentation efficiency of high-yield nattokinase strains directly determines the production volume and quality of the product.

[0003] Conventional fermentation devices for nattokinase strains often adopt conventional stirred fermentation tanks. The stirring method often depends on driving a stirring rod by an external driving device for stirring, not only has a high energy consumption, but also has poor stirring uniformity, easily causes local concentration non-uniformity of fermentation materials and insufficient contact between strains and nutrients, further restricts the fermentation efficiency. Next, the aeration device is often designed with a single aeration structure at the bottom. The diameter of the bubbles caused by aeration is large, the gas-liquid contact area is small, the oxygen utilization rate is low, and it cannot provide sufficient and stable oxygen supply for the fermentation of high-yield nattokinase strains, indirectly affecting the synthesis amount of nattokinase. To solve the above problems, a fermentation device for high-yield nattokinase strains is proposed.

Summary of the Invention

Problems to be Solved by the Invention

[0004] The main object of the present invention is to provide a fermentation device for high-yield nattokinase strains that can effectively solve the problems in the background art.

Means for Solving the Problems

[0005] To achieve the above object, the technical solution adopted by the present invention is as follows.

[0006] A fermentation apparatus for high-yielding nattokinase strains includes a fermentation tank, a support frame fitted to the outside of the fermentation tank, a bottom plate fixedly connected to the lower end of the support frame, a material supply pipe fixedly connected to the upper end of the fermentation tank, an aeration pipe fixedly connected to the lower end of the fermentation tank, an aeration assembly rotatably connected to one end of the aeration pipe, the aeration assembly provided at the lower end of the internal cavity of the fermentation tank, a stirring assembly installed above the aeration assembly, and a material discharge pipe further fixedly connected to the outside of the fermentation tank. The aeration assembly includes a rotating cavity rotatably connected to the lower end of the inner wall of the fermentation tank, the internal cavity of the rotating cavity communicating with the aeration pipe, and the rotating cavity has a plurality of branched aeration members evenly distributed along the circumferential direction.

[0007] Preferably, the branched aeration member includes a branched pipe that communicates with the internal cavity of the rotating cavity, a sleeve is fixedly connected to the end of the branched pipe away from the rotating cavity, a microbubble aeration head is screwed onto one end of the sleeve, and a plurality of micropores are provided on the outside of the microbubble aeration head.

[0008] Preferably, a filter mesh is fitted inside the sleeve, and one end of the filter mesh is fixedly connected to the branch pipe.

[0009] Preferably, the stirring assembly includes an exhaust cover fixedly connected to the upper end of the fermentation tank, wherein a plurality of helical blades are installed in the internal cavity of the exhaust cover, and a rotating rod is fixedly connected in common to one end of each of the plurality of helical blades that are close to each other, the rotating rod is rotatably connected to the fermentation tank, and a plurality of stirring rods are fixedly connected to the outside of the rotating rod.

[0010] Preferably, an I-shaped scraping frame is fixedly connected to the lower end of the rotating rod, and both vertical scraping rods of the I-shaped scraping frame are slidably connected to the fermentation tank.

[0011] Preferably, a plurality of through holes are provided at the upper end of the fermentation tank, and all of these through holes are located in the internal cavity of the exhaust cover.

[0012] Preferably, a sealing lid is screwed onto the upper end of the material supply pipe. [Effects of the Invention]

[0013] Compared to conventional technology, this invention has the following beneficial effects.

[0014] 1. This fermentation apparatus for high-yielding nattokinase strains utilizes the cooperation of a rotating cavity in the aeration assembly and multiple branched aeration members to convert sterile gas into fine bubbles by passing it through the micropores of the microbubble aeration head. This significantly increases the gas-liquid contact area, and the reaction force generated during aeration drives the rotating cavity and branched aeration members to rotate synchronously, ensuring that fine bubbles diffuse uniformly into each region of the internal cavity of the fermentation tank. This avoids the problem of uneven oxygen distribution due to localized bubble aggregation. Furthermore, the filter mesh within the branched aeration members effectively filters impurities from the gas, preventing clogging of the microbubble aeration head and ensuring the sustained stability of the aeration process, thereby further improving the yield and quality of nattokinase.

[0015] 2. This fermentation apparatus for the high-yielding nattokinase strain uses the gas generated during fermentation as power to drive a rotating rod using the gas flowing through the spiral blades inside the exhaust cover, and further drives a stirring rod to thoroughly mix the fermentation material and bubbles. This eliminates the need for additional drive equipment, significantly reducing energy consumption. Furthermore, by driving an I-shaped scraping frame with the rotating rod to rotate it synchronously, fermentation material adhering to the inner wall of the fermentation tank is scraped off, avoiding material waste and preventing the impact of scale buildup on the wall surface on the fermentation effect. [Brief explanation of the drawing]

[0016] [Figure 1] This is a schematic diagram of the overall structure of the present invention. [Figure 2] This is a cross-sectional view of the overall structure of the present invention. [Figure 3] This is a schematic diagram of the aeration assembly structure of the present invention. [Figure 4] This is a schematic diagram of the stirring assembly structure of the present invention. [Figure 5] This is a schematic diagram of the disassembled structure of the branched aeration member of the present invention. [Modes for carrying out the invention]

[0017] In order to make the technical means, creative features, objectives, and effects realized by this invention easier to understand, the invention will be further described below with reference to specific embodiments.

[0018] As shown in Figures 1-5, the fermentation apparatus for high-yielding nattokinase strains includes a fermentation tank 1, a support frame 2 fitted to the outside of the fermentation tank 1, a bottom plate 3 fixedly connected to the lower end of the support frame 2, a material supply pipe 4 fixedly connected to the upper end of the fermentation tank 1, an aeration pipe 6 fixedly connected to the lower end of the fermentation tank 1, an aeration assembly rotatably connected to one end of the aeration pipe 6, the aeration assembly provided at the lower end of the internal cavity of the fermentation tank 1, a stirring assembly installed above the aeration assembly, and a material discharge pipe 5 further fixedly connected to the outside of the fermentation tank 1.

[0019] In this embodiment, the aeration assembly includes a rotating cavity 7 rotatably connected to the lower end of the inner wall of the fermentation tank 1, the internal cavity of the rotating cavity 7 is in communication with an aeration pipe 6, and a plurality of branched aeration members 8 are evenly distributed along the circumference of the rotating cavity 7, the branched aeration members 8 include branched pipes 81 that are in communication with the internal cavity of the rotating cavity 7, a sleeve 82 is fixedly connected to the end of the branched pipe 81 away from the rotating cavity 7, a microbubble aeration head 83 is screwed onto one end of the sleeve 82, a plurality of micropores 84 are made on the outside of the microbubble aeration head 83, a filter mesh 85 is fitted inside the sleeve 82, and one end of the filter mesh 85 is fixedly connected to the branched pipe 81.

[0020] Specifically, when using this device, first, the culture medium and strain necessary for the fermentation of the nattokinase strain are sent into the internal cavity of the fermentation tank 1 via the material supply pipe 4. Then, sterile gas is injected into the rotating cavity 7 via the aeration pipe 6 using an external aeration device. At this time, the gas passes through the rotating cavity 7 and is divided into branch pipes 81 of each branch aeration member 8, where impurities are filtered by the filter mesh 85 in the sleeve 82, preventing clogging of the microbubble aeration head 83 due to impurities. At this time, the filtered gas passes through multiple fine pores 84 on the microbubble aeration head 83. This forms fine bubbles, which are uniformly dispersed at the lower end of the internal cavity of the fermentation tank 1, supplying sufficient oxygen for the fermentation of the nattokinase strain. When the microbubble aeration head 83 is ejected, a reaction force is generated, which in turn drives the rotating cavity 7 to rotate at the lower end of the inner wall of the fermentation tank 1. During the rotation of the rotating cavity 7, each branched aeration member 8 can be driven to rotate synchronously, thereby allowing the fine bubbles to diffuse more uniformly into each region of the internal cavity of the fermentation tank 1, and consequently allowing the gas and fermentation material to fuse more thoroughly, improving the utilization rate of oxygen.

[0021] In this embodiment, the stirring assembly includes an exhaust cover 9 fixedly connected to the upper end of the fermentation tank 1. In the internal cavity of the exhaust cover 9, a plurality of spiral blades 12 are installed. At one end of the plurality of spiral blades 12 close to each other, a rotating rod 11 is commonly and fixedly connected. The rotating rod 11 is rotatably connected to the fermentation tank 1. On the outer side of the rotating rod 11, a plurality of stirring rods 13 are fixedly connected. At the upper end of the fermentation tank 1, a plurality of through holes 10 are opened, and all the plurality of through holes 10 are provided in the internal cavity of the exhaust cover 9.

[0022] Specifically, the gas generated during fermentation is collected through the plurality of through holes 10 at the upper end of the fermentation tank 1 and enters the exhaust cover 9. At this time, due to the gas flow, the spiral blades 12 in the internal cavity of the exhaust cover 9 are driven to rotate. The spiral blades 12 drive the rotating rod 11 to rotate synchronously, and the plurality of stirring rods 13 on the outer side of the rotating rod 11 also rotate accordingly, sufficiently stirring and mixing the fermentation material and air bubbles in the fermentation tank 1, further promoting the fusion of the gas and the material, ensuring uniform contact between the strain, nutrients, and oxygen, effectively improving the fermentation efficiency. More specifically, since the exhaust cover 9 is a one-way exhaust cover, it can effectively prevent the intrusion of outside air and miscellaneous bacteria into the fermentation tank 1.

[0023] In this embodiment, an I-shaped scraping frame 14 is fixedly connected to the lower end of the rotating rod 11, and the two vertical scraping rods of the I-shaped scraping frame 14 are both slidably connected to the fermentation tank 1.

[0024] Specifically, when the rotating rod 11 rotates, the I-shaped scraping frame 14 is also driven to rotate synchronously. Since its two vertical scraping rods are slidably and closely attached to the inner wall of the fermentation tank 1, the fermentation material adhering to the inner wall of the fermentation tank 1 can be scraped off, thereby avoiding the waste of materials and the influence of scale adhesion on the wall surface on the fermentation effect. After fermentation is completed, the fermentation product is discharged through the material discharge pipe 5 outside the fermentation tank 1.

[0025] [[ID=​​

[0026] Specifically, the sealed lid 15 is connected to the material supply pipe 4 by a screw-on method, enabling rapid sealing and opening, and effectively preventing outside air and bacteria from entering the tank body through gaps in the material supply pipe 4 during fermentation.

[0027] This invention is a fermentation apparatus for high-yielding nattokinase strains. When using this apparatus, first, the sealed lid 15 at the upper end of the material supply pipe 4 is opened. Then, the culture medium and strain necessary for the fermentation of the nattokinase strain are sent into the internal cavity of the fermentation tank 1 via the material supply pipe 4. After the material supply is complete, the sealed lid 15 is tightened to ensure the fermentation environment is sealed and prevent the entry of unwanted bacteria. During fermentation, the external aeration equipment injects sterile gas into the rotating cavity 7 via the aeration pipe 6. The gas is then divided through the rotating cavity 7 into the branch pipes 81 of each branch aeration member 8. The filter mesh 85 inside the b 82 filters out impurities, preventing clogging of the microbubble aeration head 83. At this time, the filtered gas passes through multiple micropores 84 on the microbubble aeration head 83, forming fine bubbles that are uniformly dispersed at the lower end of the internal cavity of the fermentation tank 1, supplying sufficient oxygen for the fermentation of the nattokinase strain. When the microbubble aeration head 83 sprays gas, a reaction force is generated, which in turn drives the rotating cavity 7 to rotate at the lower end of the inner wall of the fermentation tank 1, driving each branched aeration member 8 during the rotation of the rotating cavity 7. This allows for synchronous rotation, which more uniformly diffuses fine bubbles into each region of the internal cavity of the fermentation tank 1, thereby more thoroughly fusing the gas and fermentation material and improving oxygen utilization. Furthermore, the gas generated during fermentation is collected through multiple through holes 10 at the upper end of the fermentation tank 1 and enters the exhaust cover 9. At this time, the flow of gas drives the spiral blades 12 in the internal cavity of the exhaust cover 9 to rotate, which in turn drives the rotating rod 11 to rotate synchronously. Multiple stirring rods 13 on the outside of the rotating rod 11 also rotate accordingly, thoroughly fusing the fermentation material and air bubbles in the fermentation tank 1. The mixture is stirred and mixed for minutes to further promote the fusion of gas and material, ensuring uniform contact between the bacterial strain, nutrients, and oxygen, thereby effectively improving fermentation efficiency. The rotating rod 11 rotates, and the I-shaped scraping frame 14 is also driven to rotate synchronously. The two vertical scraping rods slide and adhere closely to the inner wall of the fermentation tank 1, allowing fermentation material adhering to the inner wall to be scraped off. This avoids wasting material and preventing the fermentation effect from being affected by scale buildup on the wall. After fermentation is complete, the fermentation product is discharged through the material discharge pipe 5 on the outside of the fermentation tank 1.It is relatively practical.

[0028] The basic principles, main features, and advantages of the present invention have been described above. Those skilled in the art will understand that the present invention is not limited to the above embodiments, and that the above embodiments and specification merely describe the principles of the present invention. Within the spirit and scope of the present invention, various modifications and improvements are possible, and all such modifications and improvements fall within the claimed scope of the present invention. The scope of protection claimed by the present invention is defined by the attached utility model claims and equivalents. [Explanation of symbols]

[0029] 1...Fermentation tank, 2...Support frame, 3...Bottom plate, 4...Material supply pipe, 5...Material discharge pipe, 6...Aeration pipe, 7...Rotating cavity, 8...Branching aeration component, 81...Branching pipe, 82...Sleeve, 83...Microbubble aeration head, 84...Micropores, 85...Filter screen, 9...Exhaust cover, 10...Through hole, 11...Rotating rod, 12...Spiral blades, 13...Agitation rod, 14...I-shaped scraping frame, 15...Sealed lid.

Claims

1. A fermentation apparatus for a high-yielding nattokinase strain, comprising a fermentation tank (1), wherein a support frame (2) is fitted to the outside of the fermentation tank (1), a bottom plate (3) is fixedly connected to the lower end of the support frame (2), a material supply pipe (4) is fixedly connected to the upper end of the fermentation tank (1), an aeration pipe (6) is fixedly connected to the lower end of the fermentation tank (1), an aeration assembly is rotatably connected to one end of the aeration pipe (6), the aeration assembly is provided at the lower end of the internal cavity of the fermentation tank (1), a stirring assembly is installed above the aeration assembly, and a material discharge pipe (5) is further fixedly connected to the outside of the fermentation tank (1). The aeration assembly includes a rotating cavity (7) rotatably connected to the lower end of the inner wall of the fermentation tank (1), the internal cavity of the rotating cavity (7) communicates with the aeration pipe (6), and a plurality of branched aeration members (8) are evenly distributed along the circumferential direction within the rotating cavity (7). A fermentation apparatus for high-yielding nattokinase strains, characterized by the following features.

2. The fermentation apparatus for a high-yielding nattokinase strain according to claim 1, characterized in that the branched aeration member (8) includes a branched pipe (81) that communicates with the internal cavity of the rotating cavity (7), a sleeve (82) is fixedly connected to the end of the branched pipe (81) that is away from the rotating cavity (7), a microbubble aeration head (83) is screwed onto one end of the sleeve (82), and a plurality of micropores (84) are made on the outside of the microbubble aeration head (83).

3. The fermentation apparatus for a high-yielding nattokinase strain according to claim 2, characterized in that a filter screen (85) is fitted inside the sleeve (82), and one end of the filter screen (85) is fixedly connected to the branch pipe (81).

4. The stirring assembly includes an exhaust cover (9) fixedly connected to the upper end of the fermentation tank (1), a plurality of spiral blades (12) are installed in the internal cavity of the exhaust cover (9), a rotating rod (11) is fixedly connected to one end of each of the plurality of spiral blades (12) that are close to each other, the rotating rod (11) is rotatably connected to the fermentation tank (1), and a plurality of stirring rods (13) are fixedly connected to the outside of the rotating rod (11), characterized in that the fermentation apparatus for high-yielding nattokinase strains is as described in claim 1.

5. The fermentation apparatus for a high-yielding nattokinase strain according to claim 4, characterized in that an I-shaped scraping frame (14) is fixedly connected to the lower end of the rotating rod (11), and the two vertical scraping rods of the I-shaped scraping frame (14) are both slidably connected to the fermentation tank (1).

6. The fermentation apparatus for a high-yielding nattokinase strain according to claim 1, characterized in that a plurality of through holes (10) are made at the upper end of the fermentation tank (1), and all of the plurality of through holes (10) are provided in the internal cavity of the exhaust cover (9).

7. The fermentation apparatus for a high-yielding nattokinase strain according to claim 1, characterized in that a sealed lid (15) is screwed onto the upper end of the material supply pipe (4).

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