Decanting kit for easy component separation and extraction

Abstract

The present invention relates to a decanting kit that facilitates component separation and extraction, and more particularly, to a decanting kit that facilitates selective extraction of components that have been concentrated and separated due to differences in density during component separation of blood using a centrifuge. A decanting kit for easily separating and extracting components includes: a centrifuge container formed so that a sample placed therein can be concentrated and separated into components according to differences in density during centrifugation using a centrifuge; a decanting container attached to the top of the centrifuge container and tilted at a certain angle to decant the components separated from the centrifuge container; a plotter installed inside the centrifuge container and moving along the length of the centrifuge container to position the boundary between the centrifuged components according to differences in density between the components of the sample being centrifuged; and an insert cover installed inside the centrifuge container and for limiting the moving distance of the plotter moving along the length of the centrifuge container.

Description

[Technical field]

[0001] The present invention relates to a decanting kit that facilitates component separation and extraction, and more particularly, to a decanting kit that facilitates selective extraction of specific components that are concentrated and separated due to density differences in blood component separation using a centrifuge. [Background technology]

[0002] When a suspension containing floating materials is left alone, the denser materials will gradually sink to the bottom due to gravity, while the less dense materials will gradually move to the top; this process is called sedimentation.

[0003] In this way, when substances with different densities are mixed together, the precipitation phenomenon occurs, and over time the mixture can be separated by the density difference. In other words, the stronger the gravity, the force that separates the mixture, the greater the density difference between the mixtures becomes, so if gravity is artificially increased, the precipitation phenomenon can be accelerated.

[0004] Therefore, if we use centrifugal force instead of gravity, we can easily accelerate the sedimentation phenomenon, and this process is called centrifugation. A related centrifuge (centrifugal separator) is a device used to separate, refine, and concentrate substances with different components or specific gravity (difference in density) using the above-mentioned principle of centrifugation, and can be used for separation in medical, wastewater treatment, uranium enrichment, production, and experiments depending on the purpose of use.

[0005] Among these, medical centrifuges are used not only to separate components for analysis of blood, urine, saliva, etc., but also to extract platelet-rich plasma. Here, platelet-rich plasma (PRP) can be obtained by centrifuging complex fluids such as bone marrow and blood (hereinafter referred to as "samples"), and refers to highly enriched plasma components that are richer in platelets than general bone marrow and blood. It contains many growth factors and acts to heal wounds and regenerate damaged areas. It is used to regenerate damaged areas by injecting it into damaged areas such as ligaments and cartilage, and since it uses one's own bone marrow or blood, there are no side effects and the treatment effect is fast.

[0006] Meanwhile, the centrifuge described above is classified into various types according to the amount of sample to be centrifuged, the rotation speed, the type of rotor, etc., and includes a centrifuge container for separating and recovering components of the sample by the centrifugal force of the rotational motion of the rotor.

[0007] Based on this technical idea, a disposable container for centrifuging and processing fluid biological materials was disclosed in Korean Patent Publication No. 10-2007-0026316. However, in order to selectively extract only specific components from the components centrifuged in the container, a syringe needle was inserted into a third canola (hose) that was simply connected to the cover and reached the bottom of the container, and the components were sucked in and extracted. As the centrifuged components formed multiple layers in one chamber inside the container, the components that had been centrifuged were mixed again due to a change in the sample content in the container (a drop in the sample water level) during the sucking and extraction using the syringe, and the components had to be extracted in that state, which led to limitations in completely extracting only the specific components.

[0008] In addition, since an adhesive is used to bond the cover and the container, it is difficult to assemble and manufacture the centrifuge container. In addition, since the sample injection port and the extraction port are simply made of a rubber material, it is difficult to inject a sample or to separate and extract separated components by inserting a needle into the correct position. Summary of the Invention [Problem to be solved by the invention]

[0009] The object of the present invention is to solve the above problems and to provide a decanting kit which can easily separate and extract components by separating a sample using the centrifugal force caused by the rotation of a rotor in a centrifuge and the difference in specific gravity of each component, and can accurately separate components by using the difference in density of each component, and can easily and quickly extract a specific component from the separated components.

[0010] Another object of the present invention is to provide a decanting kit for easily separating and extracting components, which comprises a centrifugal separator for concentrating and separating components of a sample and a component recovery container for separating and storing a specific component having a low density among the concentrated and separated components, in a structure that can be disassembled and assembled mutually, thereby facilitating the concentrating and separating of components of a sample, selective extraction and recovery, and storage, thereby greatly improving the efficiency of the centrifugal separation of a sample as well as the extraction of a specific component. [Means for solving the problem]

[0011] In order to achieve the above object, the present invention provides a decanting kit for easily separating and extracting components, comprising: a centrifuge vessel formed so that a sample placed therein is concentrated and separated into components according to density differences during centrifugation using a centrifuge; a decanting vessel connected to an upper portion of the centrifuge vessel and tilted at a certain inclination to decant the components separated from the centrifuge vessel; a plotter installed inside the centrifuge vessel and moving along the length of the centrifuge vessel to position a boundary between the centrifuged components according to density differences of the components of the sample being centrifuged; and an insert cover for limiting the moving distance of the plotter, which is seated inside the centrifuge vessel and moves along the length of the centrifuge vessel.

[0012] The centrifuge vessel of the present invention comprises a centrifuge vessel body in which a certain amount of sample to be centrifuged is stored and a centrifuge chamber is formed inside so that the sample is concentrated and separated into components during centrifugation; a centrifuge vessel cover which seals the open upper end of the centrifuge vessel body and is provided with an injection port for injecting a sample into the centrifuge vessel, a vent port for discharging air when the sample is injected, and a discharge part for discharging centrifuged components; and a first packing ring which is interposed between the centrifuge vessel body and the centrifuge vessel cover to seal the inside. The centrifuge vessel body comprises a flange formed on the outer side from the upper end of the body and to which the centrifuge vessel cover is hooked, and a first packing ring formed inside the flange for sealing the centrifuge vessel cover and the body. a ring jaw on which a first packing ring is seated, and a coupling groove formed inside the ring jaw to form the same inner circumferential surface as the inner circumferential surface of the centrifuge vessel cover, the centrifuge vessel cover comprising a centrifuge vessel cover body having a cover side to be hook-coupled to the outer circumferential surface of the flange, a ring seating groove formed inside the cover side to seat the flange and the first packing ring, a coupling jaw inserted into the coupling groove to form an inner circumferential surface similar to the inner circumferential surface of the centrifuge vessel body, and a discharge part formed protruding upward so that the centrifuged sample can be decanted into a decanting vessel while forming a stable inner surface that is the same as the inner circumferential surface of the centrifuge vessel body for a certain range.

[0013] The decanting vessel of the present invention is composed of a decanting vessel body having a decanting chamber formed therein so that components discharged from the centrifuge vessel are separated, moved and stored, and a decanting vessel cover that seals the open upper end of the decanting vessel body and has a discharge port and a vent port formed therein so that components can be extracted from the decanting vessel body. One side of the decanting vessel body is formed with an inlet part having a stable inner surface like the discharge part so that low-density components discharged from the discharge part of the centrifuge vessel body can flow therethrough, and the discharge part is disposed above the cover body by a certain distance. the inlet is made of an inlet that is seated on the cover coupling step of the discharge part and has a stable inner surface like the discharge outlet, and a second packing ring is inserted between the outside of the cover coupling step and the coupling hook, and the second packing ring is inserted between the outside of the cover coupling step and the coupling hook to seal the discharge part and the inlet. Effect of the Invention

[0014] The decanting kit according to the present invention, which facilitates component separation and extraction, can concentrate and separate the constituent components of a sample into individual components using a centrifuge container, and separate and store specific components using a component recovery container, thereby facilitating the process of separating sample components and the process of selectively extracting specific components.

[0015] In addition, the present invention has the effect of preventing unwanted components from being included in the extracted components by limiting the moving distance of the plotter that moves up and down from within the centrifuge container, thereby perfectly extracting only specific components.

[0016] In addition, the present invention can separate only desired components from blood or bone marrow for use in autologous bone marrow therapy and autologous blood therapy, and can achieve medical results such as improving the therapeutic effect of the separated components (such as platelet-rich plasma), making it a very useful invention that can greatly contribute to the development of the related medical industry field. [Brief description of the drawings]

[0017] [Figure 1] 1 is a perspective view of a decanting kit for easily separating and extracting components according to the present invention; [Diagram 2] FIG. 2 is an exploded perspective view of a centrifugal separator of the decanting kit for easily separating and extracting components according to the present invention. [Diagram 3] FIG. 2 is a bottom perspective view of a centrifuge vessel cover according to the present invention. [Figure 4] FIG. 2 is an exploded perspective view of a decanting container of the decanting kit for easily separating and extracting components according to the present invention. [Diagram 5] FIG. 2 is a perspective view of the decanting vessel according to the present invention, seen from the bottom side. [Figure 6] 1 is a cross-sectional view of a decanting kit for easily separating and extracting components according to the present invention. [Figure 7] FIG. 2 is an operational diagram of the decanting kit according to the present invention, which facilitates component-by-component separation and extraction. [Figure 8-9] FIG. 2 is an exemplary view illustrating the operation of a centrifuge rotor of a decanting kit for easily separating and extracting components according to the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] The best mode for carrying out the present invention includes a centrifuge container formed so that a sample placed therein is concentrated and separated into components according to density differences during centrifugation using a centrifuge; a decanting container connected to an upper portion of the centrifuge container and inclined at a certain inclination to decant the components separated from the centrifuge container; a plotter provided inside the centrifuge container and moving along the length of the centrifuge container to be positioned at the boundary between the centrifuged components according to the density differences of the components of the sample being centrifuged; and an insert cover for restricting the moving distance of the plotter that is seated inside the centrifuge container and moves along the length of the centrifuge container.

[0019] Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings to such an extent that a person having ordinary skill in the art to which the present invention pertains can easily carry out the present invention. First, in adding reference numerals to components in the drawings, it should be noted that the same reference numerals are used for the same components as much as possible even if they are displayed in different drawings. In addition, in describing the present invention, if it is determined that a detailed description of related known configurations or functions may obscure the gist of the present invention, the detailed description thereof will be omitted.

[0020] FIG. 1 is an oblique view of a decanting kit for easily separating and extracting components according to the present invention, FIG. 2 is an exploded oblique view of a centrifuge container of the decanting kit for easily separating and extracting components according to the present invention, FIG. 3 is a bottom oblique view of a centrifuge container cover according to the present invention, FIG. 4 is an exploded oblique view of a decanting container of the decanting kit for easily separating and extracting components according to the present invention, FIG. 5 is a bottom oblique view of a decanting container of the present invention, FIG. 6 is a cross-sectional view of the decanting kit for easily separating and extracting components according to the present invention, FIG. 7 is an operation diagram of the decanting kit for easily separating and extracting components according to the present invention, and FIGS. 8 and 9 are illustrative diagrams illustrating the operation of a centrifuge rotor of the decanting kit for easily separating and extracting components according to the present invention.

[0021] As shown in FIG. 1, the decanting kit 1 for easily separating and extracting components according to the present invention includes a centrifuge container 10 in which a sample placed inside is concentrated and separated into components due to density differences during centrifugation using a centrifuge, and a decanting container 20 which is connected to the upper part of one side of the centrifuge container 10 and separates and stores components flowing out of the centrifuge container 10 which is tilted nearly horizontally due to the centrifugal force during centrifugation.

[0022] The centrifuge 3 will be described with reference to Figure 8. The centrifuge 3 is a device used for separating blood cells and plasma from blood, or for separating stem cells from bone marrow, and for separating and collecting only specific components from a complex fluid (hereinafter referred to as a "sample"), and as shown in Figures 8 and 9, a rotor 2 that rotates horizontally when the device is operated is installed inside, and a number of buckets (2-1) that rotate together with the rotor 2 when the decanting kit 1 is housed and attached are provided.

[0023] Also, as shown in FIG. 9, a hole (2-2) is formed on one side of the periphery of the bucket (2-1) for viewing inside the decanting kit 1 or for reducing the load on the back side.

[0024] The decanting kit 1 is a storage medium for concentrating and separating a sample into individual components. It is composed of a centrifuge vessel body 11 in which a certain amount of sample to be centrifuged can be stored and in which a centrifuge chamber 14 is provided in which the sample is concentrated and separated into individual components during centrifugation, a centrifuge vessel cover 12 that seals the upper open end of the centrifuge vessel body 11 but allows a sample to be injected into the centrifuge chamber 14, a plotter 13 that is provided inside the centrifuge vessel body 11 and moves along the length of the centrifuge vessel body 11 to be positioned at the boundary between each component based on the density difference of the components of the sample to be centrifuged, and an insert cover 15 that is seated inside the centrifuge chamber 14 inside the centrifuge vessel body 11 to restrict the vertical movement of the plotter 13.

[0025] 2, the centrifuge vessel 10 includes a centrifuge vessel body 11, a flange 114 formed on the outer side of the upper end of the centrifuge vessel body 11 to which the centrifuge vessel cover 12 is hooked, a ring step jaw 1113 formed on the inner side of the flange 114 to seat a first packing ring 30a for sealing the centrifuge vessel cover 12 and the centrifuge vessel body 11, and a coupling groove 112 formed on the inner side of the ring step jaw 113 to form the same inner circumferential surface as the inner side of the centrifuge vessel cover 12, and the upper end 111 of the centrifuge vessel body 11 forms the coupling groove 112 together with the ring step jaw 113. In addition, a fastening groove 116 for fixing the coupling position of the centrifuge vessel cover 12 is formed on one side of the flange 114. The fastening groove 116 can fix the mounting position of the cover 12 by inserting a fastening protrusion 121-2 formed on the centrifuge vessel cover 12.

[0026] As shown in FIGS. 2 and 3, the centrifuge vessel cover 12 includes a centrifuge vessel cover body 121 having a cover side 121-1 that is hook-coupled to the outer circumferential surface of the flange 114, a ring seating groove 129 formed on the inside of the cover side 121-1 to seat the flange 114 and the first packing ring 30a, a coupling jaw 127 that is inserted into the coupling groove 112 to form an inner circumferential surface similar to the inner circumferential surface of the centrifuge vessel body, and a discharge part 125 that forms an inner surface that is the same as the inner circumferential surface of the centrifuge vessel body 11 for a certain section and protrudes upward so that the centrifuged components can be decanted to the decanting vessel 20. The discharge part 125 is composed of a guide plate 125-1 having an inner surface that is the same as the inner circumferential surface of the centrifuge vessel body 11 for a certain section, a discharge port 125-2 having a certain shape formed by the guide plate 125-1, an upper flange 125-3 formed by extending a certain distance upward from the centrifuge vessel cover body 121, and a cover connecting step 125-4 that is extended a certain distance upward from the upper flange 125-3 to form a step on the inside. The guide plate 125-1 is formed with an inner surface that is the same as the inner circumferential surface of the centrifuge vessel body 11 for a certain section, and forms a flow path so that a specific sample centrifuged can easily move to the decanting vessel 20. The centrifuge vessel cover 12 is provided with an injection port 126 and a vent port 122, and a sample is injected into the chamber 14 of the centrifuge vessel through the injection port 126, and when the sample is injected through the injection port 126, air is discharged to the outside through the vent port 122.

[0027] 4 and 5 show the decanting vessel 20. The decanting vessel 20 is composed of a decanting vessel body 21 having a decanting chamber 23 formed therein so that the components discharged from the centrifuge vessel 10 can be separated, moved and stored, and a decanting vessel cover 22 which seals the open top end of the decanting vessel body 21 and has a discharge port 221 and a vent port 222 formed therein so that the components can be extracted from the decanting vessel body 21. The decanting vessel body 21 is provided with a decanting chamber 213 and an inlet 211. The inlet 211 is made up of an outer guide plate 211-1 hooked to the upper flange 125-3, an inner guide plate 211-2 formed the same as the guide plate 125-1, an inlet coupling groove 211-4 formed by the outer guide plate 211-1 and the inner guide plate 211-2, and an inlet 211-3 formed by the inner guide plate 211-2. The inner guide plate 211-2 is seated on the cover coupling jaw 125-4, and a second packing ring 30b is interposed between the outer surface of the cover coupling jaw 125-4 and the outer guide plate 211-1. A blunt jaw 214 is formed at the portion entering the decanting chamber 213 through the inlet 211-3, so that the centrifuged ingredients move gently to the decanting chamber 213. The decanting chamber 213 is formed with inclined surfaces 218 formed downward at a certain angle on both sides, and a storage groove 219 in which the centrifuged ingredients that have passed through the inclined surfaces 218 are stored. The upper end of the decanting container 21 is provided with an extension end 216 that extends upward by a certain length, and a decanting flange 217 formed on the outer surface of the extension end.

[0028] The decanting vessel cover 22 comprises a decanting vessel cover body 221, an outer surface part 223 extending downward from the decanting vessel cover body 221 to form a frame, and an extraction port 222 and a ventilation port 224 formed in the decanting vessel cover body 221. The outer surface part 223 of the decanting vessel cover body is hook-coupled to the decanting flange 217, and a third packing ring 30c for sealing is interposed between the outer surface part 223 coupled to the decanting flange 217 and the extension end 216.

[0029] The floater 13 prevents the precipitated components and the floating components from mixing with each other through the boundaries between the sample components concentrated and separated by components inside the centrifuge vessel body 11, and as shown in Fig. 6, the floater 13 can be configured in the form of a basket or a plate. A plurality of scaffolds 132 are formed protruding from the bottom surface of the floater bottom plate so that a sufficient gap is formed between the centrifuge vessel body 11 and the bottom plate of the floater to facilitate the precipitation of high density components below the centrifuge chamber 14 during centrifugation, and a through hole 131 is formed penetrating the center of the bottom plate of the floater so that high density components can pass through and be precipitated on the bottom surface of the bottom plate during centrifugation.

[0030] In addition, a blunt jaw portion 133 is formed protruding from the center of the upper surface of the bottom plate of the through hole 131 to facilitate the precipitation of components through the through hole 131 but to block the backflow of the precipitated components, and an expanded portion may be further formed at the upper end of the through hole 131 to facilitate the discharge of components with high density.

[0031] The operation of the decanting kit 1 according to the present invention, which is constructed as described above and allows easy component separation and extraction, will be described in detail below.

[0032] First, a sample such as blood or bone marrow taken from a patient using a syringe is transferred to a centrifuge container 10 for component separation.

[0033] More specifically, the liquid is injected into the centrifuge chamber 14 in the centrifuge vessel body 11 through an injection port portion 126 formed in the cover 12 of the centrifuge vessel 10 .

[0034] When a sample is injected into the centrifuge vessel body 11, the air remaining in the centrifuge chamber 14 is discharged to the outside through the ventilation port 122 formed in the centrifuge vessel cover 12, thereby maintaining the internal pressure of the centrifuge vessel body 11 constant.

[0035] Next, as shown in Figures 8 and 9, the decanting kit 1 of the present invention is mounted in each bucket 2-1 hinged to the rotor 2 of the centrifuge 3, and then the centrifuge 3 is driven to rotate the rotor 2 horizontally, so that the components of the sample placed in the centrifuge chamber 14 of the centrifuge container body 11 are separated in order of density from low to high by centrifugal force.

[0036] For example, in the case of blood, blood cells, which are a high density component, are concentrated and separated below the plotter 13 in the centrifugal chamber 14 of the centrifugal separator vessel body 11, and plasma, which is a low density component, is concentrated and separated above the plotter 13. The plotter 13 is subjected to buoyancy by the components with different densities and moves in the length direction.

[0037] During this process, while the sample is being separated into its components, the plotter 13 moves (floats) along the length of the centrifuge vessel body 11 due to the difference in density between the components, and is positioned at the boundary between the high and low density components. However, the plotter 13 is restricted in its movement along the length by the insert cover 15, and the presence of the insert cover 15 allows the plotter 13 to separate only a certain amount of components, thereby increasing the efficiency of the separation.

[0038] As described above, the rotor 2 rotates as the centrifuge 3 operates, and the sample in the centrifuge container 11 is centrifuged. When the centrifuge stops, a separate angle maintaining means (not shown) operates to tilt the centrifuge container 11 together with the bucket 2-1 at a certain angle, so that the components on the upper side of the plotter 13 in the centrifuge chamber 14 flow into the decanting container 20. When all the components above the plotter 13 flow into the decanting container 20, the angle maintaining means returns the bucket 2-1 to its original position, and decanting is completed.

[0039] Next, the separated components are extracted from the decanting container 20 of the decanting kit 1 by using a syringe through the extraction port 222.

[0040] The above description is merely illustrative of the technical idea of ​​the present invention, and various modifications, changes, and replacements may be made by a person having ordinary knowledge in the technical field to which the present invention belongs, as shown in FIG. 8, without departing from the essential characteristics of the present invention. Therefore, the embodiments and the accompanying drawings disclosed in the present invention are for the purpose of explanation, not for the purpose of limiting the technical idea of ​​the present invention, and the scope of the technical idea of ​​the present invention is not limited by such embodiments and the accompanying drawings. The scope of protection of the present invention should be interpreted according to the claims below, and all technical ideas within the equivalent range should be interpreted as being included in the scope of the present invention. [Industrial Applicability]

[0041] The present invention relates to a decanting kit that facilitates component separation and extraction, and in particular to a decanting kit that facilitates selective extraction of components, particularly stem cells, based on density differences in blood component separation using a centrifuge, and is an invention with great industrial applicability.

Claims

[Claim 1] A centrifuge container formed so that a sample placed inside is concentrated and separated into components according to density differences during centrifugation using a centrifuge; a decanting vessel which is connected to the upper side of the centrifugal separation vessel and inclined at a certain angle to decant the components separated from the centrifugal separation vessel; a plotter disposed within the centrifuge container, moving along a length of the centrifuge container and positioned at a boundary between components centrifuged according to density differences of the components of the sample being centrifuged; an insert cover for limiting a moving distance of a plotter that is seated inside the centrifuge container and moves along a length direction of the centrifuge container; Including, The centrifuge container includes a centrifuge container body in which a certain amount of a sample to be centrifuged is stored and a centrifuge chamber is formed inside so that the sample is concentrated and separated into components during centrifugation; a centrifuge container cover that seals an open upper end of the centrifuge container body and is provided with an injection port for injecting a sample into the centrifuge container, a vent port through which air is discharged when the sample is injected, and a discharge part through which centrifuged components are discharged; and a first packing ring that is interposed between the centrifuge container body and the centrifuge container cover to seal the inside, The centrifuge vessel body includes a flange formed on an outer side of an upper end of the body to which a centrifuge vessel cover is hook-coupled, a ring jaw formed on an inner side of the flange to which a first packing ring for sealing the centrifuge vessel cover and the body is seated, and a coupling groove formed on an inner side of the ring jaw to form the same inner circumferential surface as an inner side of the centrifuge vessel cover, The centrifuge vessel cover includes a centrifuge vessel cover body having a cover side that is hook-coupled to an outer peripheral surface of the flange, a ring seating groove formed on the inner side of the cover side to seat the flange and the first packing ring, a coupling jaw inserted into the coupling groove to form an inner peripheral surface similar to the inner peripheral surface of the centrifuge vessel body, and a discharge part formed protruding upward so that the centrifuged sample is decanted toward a decanting vessel while forming a guide surface that is the same as the inner peripheral surface of the centrifuge vessel body for a certain section, The decanting vessel includes a decanting vessel body having a decanting chamber formed therein so that the components discharged from the centrifuge vessel can be separated, moved and stored, and a decanting vessel cover that seals the open upper end of the decanting vessel body and has a discharge port and a vent port formed therein so that the components can be extracted from the decanting vessel body; An inlet portion having a guide surface similar to that of the outlet portion of the centrifugal separator body is formed on one side of the decanting container body so that low-density components discharged from the outlet portion of the centrifugal separator body can flow therethrough. The discharge part includes an upper flange having a predetermined shape formed by extending a predetermined distance upward from the cover body, a cover connecting step extending upward from the upper flange and having a step formed on its inside, and a discharge port having a predetermined shape formed inside the cover connecting step, the inlet includes an inlet that is seated on a cover coupling step of the discharge part and has a guide surface similar to the discharge outlet, and a coupling hook having a predetermined shape that surrounds an outer side of the cover coupling step and a second packing ring and is hook-coupled to the upper flange, A decanting kit for easily extracting and separating components, comprising a second packing ring interposed between the outer side of the cover joint jaw and the joint hook to seal the outlet and the inlet.

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