TGF-β synthesis inhibitor

Abstract

To provide compounding agents for cosmetics, quasi drugs or external pharmaceuticals having following effects: inhibition of abnormal accumulation of extracellular matrix induced from TGF-β, prevention and improvement of skin keloid and hypertrophic scar, and inhibition of migration from a growth period to an involutional period in a hair cycle.SOLUTION: A TGF-β synthesis inhibitor or hair loss inhibitor contains extract from Perilla frutescens, a plant of Perilla, Lamiaceae, as an active ingredient.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a TGF-β synthesis inhibitor containing an active ingredient which is derived from a plant of the genus Lamiaceae and has excellent biological safety. [Background technology]

[0002] In recent years, research has been conducted on cytokines (a type of secretory protein that regulates cell function), which are known to control cell proliferation and differentiation and promote cell death. For example, cytokines belonging to the TGF-β (Transforming Growth Factor) superfamily are known to be involved in the abnormal accumulation of extracellular matrix, keloids in the skin, hypertrophic scars, and hair growth (factors that induce the transition from the anagen phase to the regressive phase of hair), etc.

[0003] Considering the action of TGF-β within the cells, the development of various ingredients that control the synthesis of TGF-β has been investigated, but there is a demand for the development of active ingredients that are safer and more effective.

[0004] [Patent Document 1] Japanese Unexamined Patent Publication No. 60-146829 [Patent Document 2] Japanese Patent Application Publication No. 10-067674 [Patent Document 3] JP 2007-84446 A [Patent Document 4] JP 2015-172074 A DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0005] In view of the problems of the conventional techniques, the present inventors have conducted extensive research to find a new component derived from a natural product that has an inhibitory effect on the synthesis of TGF-β from the viewpoint of skin safety. As a result, they have newly discovered that an extract from Perilla frutescens (Lamiaceae, Lamiaceae) has an inhibitory effect on TGF-β1. [Means for solving the problem]

[0006] The present invention relates to a TGF-β1 synthesis inhibitor containing, as an active ingredient, an extract from Perilla, a plant of the Lamiaceae family. The present invention relates to a hair loss inhibitor containing an extract of perilla, a plant of the genus Lamiaceae, as an active ingredient. Effect of the Invention

[0007] The present invention relates to a TGF-β1 synthesis inhibitor containing as an active ingredient an extract from Perilla, a plant of the Lamiaceae family, and the present invention is capable of inhibiting abnormal accumulation of extracellular matrix, preventing and improving keloids and hypertrophic scars of the skin, and inhibiting hair loss and improving firmness and strength associated with maintaining hair growth. [Brief description of the drawings]

[0008] [Figure 1] FIG. 1 is a graph showing the TGF-β1 synthesis inhibitory effect of the extract according to the present invention.

[0009] Preferred embodiments of the present invention will now be described in detail. The extraction material used in the present invention is perilla of the genus Perilla in the family Lamiaceae, and generally includes those represented by the scientific names Perilla frutescens var. crispa or Perilla frutescens var. Britton, such as perilla green, perilla chirimen shiso, perilla rosa, perilla madara, perilla katamen shiso, perilla chirimen aojiso, or varieties, subspecies, or hybrids thereof, but the present invention is not limited thereto.

[0010] The material used in the present invention may be any of the whole plant, leaves, flowers, stems, seeds, fruits, roots, etc. of perilla of the Lamiaceae family, but it is preferable to use the whole plant, leaves, flowers, stems and / or fruits, and it is particularly preferable to use the leaves and / or flowers. Furthermore, there are no particular limitations on the harvest time and size of the parts of perilla used, and any size or harvest time may be used.

[0011] The preparation of the extract involves first washing a part of perilla (e.g., the whole plant, or the leaves, flowers, stems and / or fruits) of the Lamiaceae family (Lamiaceae) to remove foreign matter, leaving it as is or drying it, and then shredding or crushing it as necessary, and contacting it with an extraction solvent to carry out extraction. The extraction can be carried out by contacting it with the extraction solvent according to a conventional method such as the immersion method, but it is also possible to use a supercritical extraction method or a steam distillation method.

[0012] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these can be used alone or in combination of two or more.

[0013] Among these extraction solvents, in the present invention, water or a mixed solvent of water and lower alcohols or polyhydric alcohols is preferred from the viewpoint of the effectiveness and skin irritation of the obtained extract, and also from the viewpoint of wide application to products such as skin external preparations.Preferred examples of the hydrophilic solvent include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol, glycerin), among which water alone or a mixed solvent of water and 1,3-butylene glycol is particularly preferred.

[0014] When a mixed solvent is used, the mixing ratio is preferably in the range of, for example, a volume ratio (hereinafter the same) of 1:1 to 20:1 for a mixed solvent of water and 1,3-butylene glycol, 1:1 to 25:1 for a mixed solvent of water and ethanol, and 1:1 to 20:1 for a mixed solvent of water and glycerin.

[0015] The weight ratio of a dried part of perilla of the Lamiaceae family (e.g., the whole plant, or the leaves, flowers, stems and / or fruits) to the extraction solvent is preferably in the range of 1:1 to 1:50, and more preferably in the range of 1:5 to 1:20.

[0016] In preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 4 to 9. In this sense, if necessary, an alkalinity regulator such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be blended with the extraction solvent to adjust the pH to a desired level.

[0017] The extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, when water or a mixture of water and 1,3-butylene glycol or ethanol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, and the extraction time is preferably 1 hour to 3 days.

[0018] The extract prepared as described above may be used as a cosmetic ingredient as it is after generally adjusting the pH to 4 to 8, or may be used at a desired concentration by concentrating under reduced pressure, etc. The extract may also be dried by a conventional method such as spray drying.

[0019] The extract prepared as described above may be used after refrigerating for a certain period of time in order to improve storage stability, etc. The extract prepared as described above may be used after adding a lower alcohol such as ethanol or a polyhydric alcohol (1,3-butylene glycol, propanediol, etc.) for the purpose of preservation, improving stability, etc.

[0020] The perilla extract of the present invention can be used in cosmetics, quasi-drugs, or pharmaceuticals, for example, ointments, creams, lotions, essences, milky lotions, packs, lipsticks, foundations, liquid foundations, make-up press powders, blushers, face powders, face washes, body shampoos, soaps, bath additives, etc., but the present invention is not limited thereto.

[0021] When the present invention is blended into cosmetics or quasi-drugs, the solid content of the extract is generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight, for basic cosmetics, generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight, for makeup agents, generally 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight, for cleansing cosmetics. When the extract according to the present invention is blended into hair cosmetics (including quasi-drugs), for example, for hair growth and nourishing cosmetics, the solid content is generally 0.00001 to 5.0% by weight (solid content weight%, the same applies below), preferably 0.001 to 3.0% by weight. For hair washing cosmetics such as shampoo, the content is generally 0.00001 to 5.0% by weight (% by weight of solids, the same applies below), and preferably 0.0001 to 1.0% by weight. For rinses and conditioners, the content is generally 0.00001 to 5.0% by weight (% by weight of solids, the same applies below), and preferably 0.001 to 1.0% by weight.

[0022] When the present invention is incorporated into cosmetics, quasi-drugs, or topical drugs, ingredients that are usually used in such preparations, such as oily ingredients, surfactants (synthetic and natural), moisturizers, thickeners, preservatives and disinfectants, powder ingredients, antioxidants, chelating agents, pH adjusters, colorants, fragrances, etc. may be incorporated as needed. In addition, there is no problem with using it in combination with other physiologically active ingredients, so long as the effectiveness and characteristics are not impaired.

[0023] Here, examples of oily components include oils and fats derived from plants, such as lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadowfoam oil, rosehip oil, lambender oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, and plant-derived squalane; oils and fats derived from animals, such as mink oil and turtle oil; waxes, such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. Examples of suitable fatty acids include hydrocarbons such as vinyl wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0024] Examples of the surfactant include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of usable surfactants include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, and N-acylamidopropyl-N',N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0025] Examples of the emulsifier or emulsification aid that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, soybean-derived protein and polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), and the like.

[0026] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extracts, Bletilla serrata root (white) extracts, various amino acids and their derivatives.

[0027] Examples of thickening agents include ingredients derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; Bletilla serrata root (white) extract; polysaccharides such as pectin, locust bean gum, aloe polysaccharide, and Alcaligenes polysaccharide; gums such as xanthan gum, tragacanth gum, and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, and acrylic acid-methacrylic acid copolymer; hyaluronic acid and its derivatives; and polyglutamic acid and its derivatives.

[0028] Examples of preservatives and disinfectants include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazolidinyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, bark distillate, radish fermentation liquid, ethanol derived from plants such as sugar cane, and 1,3-butylene glycol.

[0029] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybeans, adzuki beans, etc.), and the like.

[0030] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0031] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, extract of Brussels sieboldii, extract of Bletilla serrata root, extract of Peony root, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate, etc.), and the like.

[0032] Examples of the chelating agent include trisodium ethylenediaminehydroxyethyltriacetate, edetic acid or its salts, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, and tetrasodium hydroxyethanediphosphonate.

[0033] Examples of pH adjusters include citric acid or salts thereof, lactic acid or salts thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, and potassium hydroxide.

[0034] Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, and AMP (adenosine monophosphate), which may be used alone or in combination.

[0035] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid esters such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate. Ascorbic acid sugar derivatives such as L-ascorbic acid ester salts, L-ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, etc., 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc. Ascorbic acid tetra fatty acid esters, 3-O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-sodium palmitate, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc., and hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside). amide), α-arbutin (hydroquinone-α-D-glucopyranoside), etc.; tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), tranexamic acid amides (e.g., tranexamic acid methylamide), etc.; resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, etc.; 2,5-dihydroxybenzoic acid derivatives include, for example, 2,Examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0036] Examples of physiologically active ingredients include placenta extract, mulberry bark extract, saxifrage extract, rice bran extract or hydrolysate thereof, white mustard extract or hydrolysate thereof, fermented white mustard, peony extract or hydrolysate thereof, lactic acid bacteria fermented rice, purple barley extract, lotus flower extract, Job's tears hydrolysate, Job's tears seed fermented product, royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract, and chamomile extract. In addition, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolysate, apricot fruit extract, seaweed extract such as Eucalyptus tschonoskii, extract of marine flowering plants such as Zostera marina, fermented soy milk, jellyfish water, rice extract or its hydrolysate, fermented rice extract, germinated rice extract or its hydrolysate, fermented germinated rice, black soybean extract or its hydrolysate, extract of Damask rose flower, extract of bamboo shoot skin, linoleic acid and its derivatives or processed products (e.g. liposomal linoleic acid, etc.), collagen derived from animals or fish and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, gamma-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or its fermented product, red ginseng extract, Mitsuishi Kami extract, loofah extract, Ulva pertusa extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, pau d'arco bark extract, hibiscus flower extract or fermented product, silverleaf extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red fuki extract, striated lanthanum extract, Japanese pepper peel or seed coat extract or hydrolysate, safflower flower extract, Casablanca extract, sweet potato extract or its fermented product, guava leaf extract, Houttuynia cordata extract, Banpeiyu extract, aloe extract, fig extract, apple extract, white asparagus extract, etc.

[0037] In addition, when the present invention is used in a preparation for inhibiting hair loss, it may be used in combination with an ingredient that is expected to have a synergistic effect on hair growth and nourishment. For example, such ingredients include minoxidil, cyproterone acetate, pentadecanoic acid glyceride, 6-aminobenzylpurine (cytopurine), adenosine, trans-3,4'-dimethyl 3-hydroxyflavanone (t-flavanone), Swertia japonica extract, hinokitiol, photosensitizer, pantothenic acid and its derivatives, vitamin E and its derivatives, nicotinic acid derivatives (nicotinamide, etc.), carpronium chloride, female hormones (ethynylestradiol, estrone, etc.), salicylic acid, dipotassium glycyrrhizinate, hinokitiol, benzalkonium chloride, isopropyl methylphenol, l-menthol, salts, etc. Examples of effective active ingredients include pyridoxine hydrochloride (vitamin B6), thioxolone, camphor, resorcinol, extract of Polytrichum formosum root, bis-alkaloids obtained from Polytrichum formosum, licorice extract, Swertia japonica extract, snail flower extract, chamomile extract, fermented royal jelly, fermented lotus seed, ginkgo extract, Paldarco bark extract, gentiana extract, ginseng extract, fermented soy milk, hydrolyzed black soybean extract, Salicornia herb extract, bamboo shoot extract, kudzu root extract, Laminaria acutiloba extract, clove extract, collagen, amino acids, and vitamins, and any one or more of these may be blended.

[0038] In particular, it is preferable to use it in combination with at least one of the following: dipotassium glycyrrhizinate, licorice extract, paldarco bark extract, or gentian extract, which suppress inflammation of the scalp; isopropyl methylphenol, which has the effect of suppressing inflammation of the scalp and a bactericidal effect; Tamasakisuzurafuji root extract or a bis-alkaloid obtained therefrom, which has a blood flow promoting effect; Panax ginseng extract, which has a blood flow promoting effect and a sebum secretion inhibiting effect; Swertia jasmine extract, which has a blood circulation promoting effect; fermented soy milk broth, which has an inhibitory effect on sebum oxidation; Salicornia herb extract, which restores the barrier function of the scalp; bamboo shoot extract or kudzu root extract, which promotes the transition to the hair growth phase; Mitsuishikonbu extract, which suppresses hair loss; and collagen, which suppresses dry scalp.

[0039] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples and Test Examples, but the present invention is not limited thereto. In the following, all parts are by weight and all % are by weight.

[0040] Manufacturing Example 1. Preparation of Perilla Leaf Extract (1) 400 g of purified water was added to 40 g of dried perilla leaves and extracted for 18 hours at 10° C. This was filtered to obtain 337 g of a brown extract (solid content 2.3%).

[0041] Production Example 2. Preparation of whole plant extract of perilla (2) 400g of purified water was added to 40g of dried perilla whole plant, and the mixture was extracted overnight (18 hours) in a refrigerator (2-10℃). This was filtered to obtain 300g of a brown extract (solid content 2.0%).

[0042] Formulation example 1. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Butylparaben 0.1 Extract of Preparation Example 1 5.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts) Fragrance (appropriate amount)

[0043] Formulation example 2: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract from Preparation Example 2 was used instead of the extract from Preparation Example 1 contained in Formulation Example 1.

[0044] Formulation example 3. Emulsion [Ingredients] Part Liquid Paraffin 6.0 Hexalan 4.0 Jojoba oil 1.0 Lotus essential oil 0.025 Polyoxyethylene (20) Sorbitan Monostearate 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Extract of Preparation Example 1 3.0 L-Ascorbic Acid 2-Glucoside 2.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Water-soluble collagen 0.1 Purified water (enough to make the total amount 100 parts) Fragrance (appropriate amount)

[0045] Formulation example 4: Lotion [Ingredients] Part Extract of Preparation Example 1 10.0 Ethanol 10.0 Glycerin 3.0 1,3-Butylene glycol 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Xanthan gum 0.1 Guar gum 0.1 Fragrance (appropriate amount) Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0046] Formulation example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 4, except that 10.0 parts of the extract of Preparation Example 2 was used in place of the extract of Preparation Example 1 among the ingredients of Formulation Example 4.

[0047] Prescription example 6. Essence [Ingredients] Part Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Hyaluronic acid 0.1 Hydrolyzed hyaluronic acid liquid 0.1 Extract of Preparation Example 1 5.0 Citric acid 0.3 Sodium citrate 0.6 Purified water (enough to make the total amount 100 parts)

[0048] Formulation example 7. Body shampoo [Ingredients] Part Sodium N-lauroylmethylalanine 25.0 Potassium coconut oil fatty acid solution (40%) 26.0 Coconut oil fatty acid diethanolamide 3.0 Methylparaben 0.1 Extract of Preparation Example 1 5.0 1,3-Butylene glycol 2.0 Purified water (enough to make the total amount 100 parts)

[0049] Prescription example 8. Hair growth agent [Ingredients] Part l-Menthol 0.8 Adenosine 1.0 Extract of Preparation Example 1 2.0 1,3-Butylene glycol 10.0 Phenoxyethanol 0.2 Ethanol 20.0 Purified water (enough to make the total amount 100 parts)

[0050] Prescription example 9. Hair growth agent A hair growth agent was obtained in the same manner as in Formulation Example 8, except that minoxidil was used instead of adenosine among the ingredients in Formulation Example 8.

[0051] Prescription example 10. Hair growth agent A hair growth agent was obtained in the same manner as in Formulation Example 9, except that, among the ingredients of Formulation Example 8, 6-benzylaminopurine was used instead of adenosine.

[0052] Prescription example 11. Hair growth agent [Ingredients] Part Dipotassium glycyrrhizinate 0.1 l-Menthol 0.1 Extract of Preparation Example 1 1.0 Soy lecithin 0.2 1,3-Butylene glycol 10.0 Ethanol 20.0 Bamboo Shoot Skin Extract 3.0 Brown algae extract 3.0 Salicornia extract 3.0 Gentian Extract 3.0 Zostera extract 3.0 Purified water (enough to make the total amount 100 parts)

[0053] Prescription example 12. Hair growth agent A hair growth agent was obtained in the same manner as in Formulation Example 11, except that pueraria root extract was used instead of bamboo shoot extract and pau d'arco bark extract was used instead of gentian extract.

[0054] Prescription example 13. Hair growth agent A hair growth agent was obtained in the same manner as in Formulation Example 11, except that a black soybean hydrolyzed extract was used instead of the eelgrass extract, and a soybean fermented liquid was used instead of the Salicornia herbacea extract.

[0055] Formulation example 14. Hair cream [Ingredients] Part Liquid Paraffin 15.0 Vaseline 15.0 White Beeswax 2.0 Extract of Preparation Example 1 1.0 Brown algae extract 0.3 Carboxyvinyl polymer 0.1 Xanthan gum 0.1 Glycerin 5.0 1,3-Butylene glycol 2.0 Polyoxyethylene hydrogenated castor oil 3.0 Chelating agent 0.1 Preservatives: 0.1 Fragrance 0.1 Dye 0.01 Purified water (enough to make the total amount 100 parts)

[0056] Formulation example 15. Hair shampoo [Ingredients] Part Sodium N-cocoyl methyl taurate 10.0 Sodium polyoxyethylene (3) alkyl ether sulfate 20.0 Lauryl dimethylaminoacetate betaine 10.0 Coconut oil fatty acid diethanolamide 4.0 Methylparaben 0.1 Citric acid 0.1 Extract of Preparation Example 1 2.0 Collagen 5.0 Zostera extract 5.0 Black soybean hydrolyzate 5.0 Salicornia extract 5.0 Pau D'arco Bark Extract 5.0 Kudzu Root Extract 5.0 1,3-Butylene glycol 2.0 Purified water (enough to make the total amount 100 parts)

[0057] Formulation example 16. Hair shampoo A hair shampoo was obtained in the same manner as in Formulation Example 15, except that the extract of Preparation Example 2 was used instead of the extract of Preparation Example 1 among the ingredients of Formulation Example 15.

[0058] Example 17. Hair rinse [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Cetanol 3.2 Stearyl alcohol 1.0 Methylparaben 0.1 Extract of Preparation Example 1 2.0 1,3-Butylene glycol 5.0 Purified water (enough to make the total amount 100 parts)

[0059] Test Example 1. TGF-β1 synthesis inhibitory effect test Rabbit corneal-derived cells (SIRC) were cultured in Eagle's minimum essential medium (Nissui Pharmaceutical Co., Ltd.) containing 10% FBS at a concentration of 5 × 10 4 The cells were adjusted to a concentration of 100 cells / mL, 100 μL of which were seeded on a 96-well microplate and cultured at 37°C under 5% carbon dioxide and saturated water vapor. After culturing for 3 days, the medium was replaced with Eagle's minimum essential medium containing 10% FBS and the extract of Production Example 1 to a final concentration of 2%, and culture was continued. In addition, a test group was set as a control, where Eagle's minimum essential medium containing 10% FBS and PBS(-) was added to a final concentration of 2%. On the day after adding the samples, a UV-B lamp (Philips TL20W / 12RS) was used to illuminate the bottom of the culture vessel at approximately 100 mJ / cm. 2 The cells were exposed to ultraviolet light for 48 hours, and the supernatant was collected 48 hours later. The cytokine (TGF-β1) secreted into the supernatant was measured using an antibody array kit (RayBiotech). The results were obtained by chemiluminescence, so the images were analyzed and the values ​​were calculated from the brightness.

[0060] The results of Test Example 1 are shown in FIG. As shown in Figure 1, it was confirmed that the extract of the present invention has a significantly excellent effect of promoting TGF-β1 synthesis. This suggests that the extract of the present invention has an effect of preventing and improving abnormal accumulation of extracellular matrix, keloids and hypertrophic scars of the skin. It is also suggested that the extract of the present invention inhibits the transition from the growth phase to the regression phase of hair in hair follicle cells, inhibits hair loss, and improves the firmness and strength of hair.

Claims

1. A TGF-β synthesis inhibitor containing, as an active ingredient, a low-temperature (0°C to 10°C) water extract of the leaves of perilla, a plant of the genus Perilla in the family Lamiaceae.

2. A hair loss inhibitor comprising the TGF-β synthesis inhibitor according to claim 1.

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