Repairing and anti-aging composition specially researched for dry sensitive skin and application of repairing and anti-aging composition

Abstract

The invention provides a repairing and anti-aging composition specially researched for dry sensitive skin and application of the repairing and anti-aging composition, and belongs to the technical field of cosmetics. According to the anti-aging composition disclosed by the invention, inositol, ceramide-NP, phytosphingosine, a golden camellia extract, a waxberry bark extract and a fructus cnidii extract are selected according to a specific mass ratio, and the six components are combined for use, so that a strong anti-aging network can be formed, and a plurality of aspects act together, so that the anti-aging composition has a good anti-aging effect. The permeation promoting, repairing, moisturizing, anti-aging and soothing effects of the anti-aging composition are improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of cosmetics, and in particular to a repairing and anti-aging composition specially developed for dry and sensitive skin and its application. Background Art

[0002] Dry sensitive skin is a common skin type, usually characterized by dryness, itching, redness, swelling, and high sensitivity to external stimuli. The characteristics of this skin type are mainly due to the damage to the epidermal barrier function, which leads to water loss and reduced ability of the skin to resist external stimuli. Due to insufficient moisture and damaged barrier, dry sensitive skin is more prone to aging phenomena such as fine lines, wrinkles, and sagging, increasing the urgent demand for anti-aging products.

[0003] In the existing technology, many anti-aging products focus on using highly active ingredients such as retinol, peptides and antioxidants to promote skin cell regeneration and reduce wrinkles, but many of these ingredients may irritate dry and sensitive skin, causing redness or burning sensations; while traditional moisturizing ingredients such as glycerin and hyaluronic acid can effectively moisturize, they have limited effects on deep repair of the skin; in addition, many existing products have added ingredients such as fragrances and alcohol to their formulas, which may aggravate skin irritation and dryness. Therefore, consumers with dry and sensitive skin often face a dilemma between effectiveness and safety when using anti-aging products. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and to provide a repair and anti-aging composition specially developed for dry and sensitive skin and its application.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is as follows: In the first aspect, an anti-aging composition is provided, which comprises the following components in parts by weight: 0.1-3 parts of inositol, 0.01-1 parts of ceramide-NP, 0.01-1 parts of phytosphingosine, 0.1-5 parts of camellia chrysanthemi extract, 0.1-5 parts of bayberry bark extract, and 0.1-5 parts of cnidium monnieri extract.

[0006] In some embodiments, the anti-aging composition comprises the following components in parts by weight: 0.5-2 parts of inositol, 0.05-0.5 parts of ceramide-NP, 0.05-0.5 parts of phytosphingosine, 0.5-3 parts of camellia chrysantha extract, 0.5-3 parts of bayberry bark extract, and 1-4 parts of cnidium monnieri extract.

[0007] In some embodiments, the anti-aging composition comprises the following components in parts by weight: 1-1.5 parts of inositol, 0.1-0.2 parts of ceramide-NP, 0.2-0.3 parts of phytosphingosine, 1-2 parts of camellia chrysantha extract, 1-2 parts of bayberry bark extract, and 2-3 parts of cnidium monnieri extract.

[0008] In some embodiments, in the anti-aging composition, the mass percentage of the Camellia chrysantha extract is 14-30%.

[0009] In some embodiments, the mass percentage of myricitrin in the bayberry bark extract is 5-15%; and / or the mass percentage of osthole in the cnidium monnieri extract is 2-10%.

[0010] In some embodiments, the method for preparing the Camellia chrysantha extract comprises the following steps:

[0011] The flowers of Camellia chrysantha are dried, crushed, and passed through a 80-150 mesh sieve to obtain Camellia chrysantha powder;

[0012] The camellia chrysantha powder is added into an ethanol solution for extraction and concentrated into a paste to obtain the camellia chrysantha extract; the solid-liquid ratio of the camellia chrysantha powder to the ethanol solution is 1g:10-30mL.

[0013] In some embodiments, the volume fraction of the ethanol solution is 60-90%; and / or the extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 30-40°C, the time of ultrasonic extraction is 20-50min, and the power of ultrasonic extraction is 80-200W.

[0014] In a second aspect, a use of the anti-aging composition in the preparation of cosmetics is provided.

[0015] In a third aspect, a cosmetic is provided, the cosmetic comprising the anti-aging composition, wherein the content of the anti-aging composition in the cosmetic is 1-20 wt %.

[0016] In some embodiments, the cosmetic is a facial cream, and the facial cream includes the following components in percentage by weight: 1-20% anti-aging composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH adjuster, and the balance water.

[0017] In some embodiments, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0018] And / or, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0019] And / or, the pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium;

[0020] And / or, the emulsifier includes at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0021] And / or, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: the anti-aging composition disclosed in the present invention selects inositol, ceramide-NP, phytosphingosine, camellia chrysantha extract, bayberry bark extract and cnidium monnieri extract in a specific mass ratio. The six components are used in combination to form a powerful anti-aging network, which works together from multiple aspects to improve the penetration, repair, moisturizing, anti-aging and soothing effects of the anti-aging composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a comparison of eye wrinkles of the blank application example and application example 1 in effect example 4 before use and after 28 days of use. DETAILED DESCRIPTION

[0024] In order to facilitate understanding of the present disclosure, the present disclosure will be described more fully below. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present disclosure more thorough and comprehensive.

[0025] As used herein:

[0026] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0027] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0028] When amount, concentration or other value or parameter is represented by range, preferred range or a series of upper preferred value and lower preferred value, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1-5", described range should be interpreted as including range "1-4", "1-3", "1-2", "1-2 and 4-5", "1-3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0029] In these examples, parts and percentages are by mass unless otherwise indicated.

[0030] "Parts by mass" refers to the basic unit of measurement for expressing the mass ratio of multiple components. 1 part can represent any unit mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it means that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number, indicating a multiple factor). It should not be misunderstood that, unlike the mass parts, the sum of the mass of all components is not limited to 100 parts.

[0031] "And / or" is used to indicate that one or both of the stated situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0032] In order to solve the dilemma between the irritation and the anti-aging effect of the anti-aging composition for dry and sensitive skin.

[0033] In a first aspect of the present disclosure, an anti-aging composition is provided, comprising the following components in parts by weight: 0.1-3 parts of inositol, 0.01-1 parts of ceramide-NP, 0.01-1 parts of phytosphingosine, 0.1-5 parts of camellia chrysantha extract, 0.1-5 parts of bayberry bark extract, and 0.1-5 parts of cnidium monnieri extract.

[0034] The anti-aging composition disclosed in the present invention selects inositol, ceramide-NP, phytosphingosine, golden camellia extract, bayberry bark extract and cnidium monnieri extract in a specific mass ratio. The six components are used in combination to form a powerful anti-aging network, which works together from multiple aspects to improve the penetration-promoting, repairing, moisturizing, anti-aging and soothing effects of the anti-aging composition. Among them, inositol, ceramide-NP and phytosphingosine are added to target the key lipid components lacking in dry and sensitive skin. Inositol is a precursor of phosphatidylinositol and its metabolites in human skin, which helps to repair damaged skin lipid layers, increase the skin's moisturizing ability, promote the healthy growth of skin cells, and delay the aging process; ceramide-NP is an important skin barrier component that can directly repair the skin barrier damaged by dehydration, reduce the damage to the skin caused by external stimuli, relieve discomfort symptoms such as dryness and redness, and has anti-aging effects, reducing the appearance of fine lines and wrinkles; phytosphingosine keeps the skin soft and smooth by enhancing the skin's own defense capabilities; while inositol, ceramide-NP and phytosphingosine have low absorption in dry and sensitive skin, the present disclosure found that adding camellia chrysantha extract to the anti-aging composition can promote the absorption of the above three components and increase their content in dry and sensitive skin. The synergistic effect of the four enables the skin to effectively resist external stimuli and water loss, and enhances the skin barrier of dry and sensitive skin. Bayberry bark extract is rich in myricitrin, which has strong antioxidant and anti-inflammatory effects, and can specifically remove senescent cells, promote the regeneration of skin cells, and improve skin elasticity and firmness, further increasing the anti-aging and soothing effects of the anti-aging composition; Cnidium monnieri extract is rich in osthole, which has good anti-inflammatory and anti-allergic effects, can inhibit skin itching and allergic reactions, and reduce the discomfort caused by dry and sensitive skin. It works together with the above components to further improve the repair, moisturizing and soothing effects of the anti-aging composition.

[0035] For example, in different embodiments, the weight portion of the inositol can be but is not limited to 0.1, 0.2, 0.5, 0.7, 1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7, 3, preferably 0.5-2, more preferably 1-1.5.

[0036] For example, in different embodiments, the weight proportion of the ceramide-NP may be, but is not limited to, 0.01, 0.05, 0.1, 0.2, 0.5, 0.7, or 1, preferably 0.05-0.5, and more preferably 0.1-0.2.

[0037] For example, in different embodiments, the weight portion of the phytosphingosine can be, but is not limited to, 0.01, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, or 1, preferably 0.05-0.5, and more preferably 0.2-0.3.

[0038] For example, in different embodiments, the weight portion of the Camellia chrysantha extract can be but is not limited to 0.1, 0.3, 0.5, 0.7, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or 5, preferably 0.5-3, and more preferably 1-2.

[0039] For example, in different embodiments, the weight portion of the bayberry bark extract can be but is not limited to 0.1 parts, 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, preferably 0.5-3 parts, and more preferably 1-2 parts.

[0040] For example, in different embodiments, the weight proportion of the Cnidium monnieri extract can be, but is not limited to, 0.1, 0.5, 0.8, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or 5, preferably 1-4, and more preferably 2-3.

[0041] The present disclosure study found that the weight of the components in the anti-aging composition will affect the comprehensive performance of the anti-aging composition. When the weight of each component is within the above preferred range, the obtained anti-aging composition has better effects in enhancing the skin barrier, alleviating sensitivity and anti-aging.

[0042] In some embodiments, in the anti-aging composition, the mass percentage of the Camellia chrysantha extract is 14-30%, for example, but not limited to 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%.

[0043] The present disclosure study found that in the anti-aging composition, the mass percentage of Camellia chrysantha extract not only affects the penetration and absorption of the anti-aging composition, but also affects the anti-aging effect of the anti-aging composition. When the mass percentage of Camellia chrysantha extract is further selected to be within the above-mentioned range, the comprehensive effect of the anti-aging composition obtained is better, and the soothing, repairing and anti-aging effects on dry and sensitive skin are better.

[0044] In some embodiments, the weight percent content of myricitrin in the bayberry bark extract is 5-15%; for example, it can be but not limited to 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%.

[0045] In some embodiments, the mass percentage of osthole in the Cnidium monnieri extract is 2-10%, for example, but not limited to 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%.

[0046] The present disclosure has found that when the mass percentage of myricitrin in the bayberry bark extract or the mass percentage of osthole in the cnidium monnieri extract is further selected within the above range, it can not only further enhance the anti-aging effect of the anti-aging composition and the soothing effect on dry skin.

[0047] In some embodiments, the method for preparing the Camellia chrysantha extract comprises the following steps:

[0048] The flowers of Camellia chrysantha are dried, crushed, and passed through a 80-150 mesh sieve to obtain Camellia chrysantha powder;

[0049] The camellia chrysantha powder is added into an ethanol solution for extraction and concentrated into a paste to obtain the camellia chrysantha extract; the solid-liquid ratio of the camellia chrysantha powder to the ethanol solution is 1g:10-30mL.

[0050] For example, in different embodiments, the ratio of the Camellia chrysantha powder and the ethanol solution may be, but is not limited to, 1g:10mL, 1g:15mL, 1g:20mL, 1g:25mL, 1g:30mL;

[0051] For example, in different embodiments, the mesh size of the Camellia chrysantha powder is 80-150 mesh; for example, it can be but not limited to 80 mesh, 100 mesh, 120 mesh, and 150 mesh.

[0052] For example, in different embodiments, the volume fraction of the ethanol solution is 60-90%, for example, it can be but not limited to 60%, 65%, 70%, 75%, 80%, 85%, 90%.

[0053] For example, in different embodiments, the power of the ultrasonic extraction is 80-200W; for example, it can be but is not limited to 80W, 100W, 120W, 140W, 160W, 180W, 200W.

[0054] For example, in different embodiments, the ultrasonic extraction time is 20-50 min; for example, it can be but not limited to 20 min, 25 min, 30 min, 35 min, 40 min, 45 min, 50 min;

[0055] For example, in different embodiments, the temperature of the ultrasonic extraction is 30-40°C; for example, it can be but not limited to 30°C, 32°C, 34°C, 36°C, 38°C, 40°C.

[0056] The present disclosure study found that the preparation method of Camellia chrysantha extract will affect the content and type of active ingredients in the Camellia chrysantha extract, and further affect its interaction with other components. The inventors found in the actual research process that the Camellia chrysantha extract prepared using the above parameters can significantly improve the absorption of inositol, ceramide-NP and phytosphingosine, and increase the content of the three in the skin, so that the anti-aging composition has a higher repair, anti-aging and sensitivity relief effect.

[0057] In a second aspect, a use of the anti-aging composition in the preparation of cosmetics is provided.

[0058] In a third aspect, a cosmetic is provided, comprising the anti-aging composition, wherein the content of the anti-aging composition in the cosmetic is 1-20wt%, for example, but not limited to 1wt%, 3wt%, 5wt%, 7wt%, 9wt%, 11wt%, 13wt%, 15wt%, 18wt%, 20wt%.

[0059] In addition to the above-mentioned extracts, the composition used according to the invention generally comprises a physiologically acceptable and preferably cosmetically acceptable medium, meaning a medium suitable for contact with human skin without toxicity, incompatibility, instability or allergic reaction, in particular without causing discomfort (redness, tightness, stinging). Advantageously, the cosmetic can be in the form of a powder, emulsion, microemulsion, nanoemulsion, suspension, solution, lotion, cream, aqueous or hydroalcoholic gel, foam, solution or dispersion for aerosol or lipid vesicle dispersion. In the case of an emulsion, it can be a water-in-oil or oil-in-water emulsion.

[0060] In addition to the anti-aging composition of the present disclosure, the cosmetic may further comprise:

[0061] One or more moisturizing agents, for example, selected from amino acids, chondroitin sulfate, diglycerol, erythritol, fructose, glucose, glycerol, glycerol polymers, ethylene glycol, 1,2,6-hexanetriol, honey, hyaluronic acid, hydrogenated honey, hydrogenated starch hydrolysate, inositol, lactitol, maltitol, maltose, mannitol, natural moisturizing factor, PEG-15 butylene glycol, polyglyceryl sorbitol, salts of pyrrolidone carboxylic acid, PCA potassium, propylene glycol, sodium glucuronate, PCA sodium, sorbitol, sucrose, trehalose, urea, xylitol and mixtures thereof.

[0062] In some aspects of the present invention, the cosmetics do not include emulsifiers. However, in other aspects, the cosmetics may include one or more emulsifiers. Non-limiting examples include esters of glycerol, esters of propylene glycol, fatty acid esters of polyethylene glycol, fatty acid esters of polypropylene glycol, esters of sorbitol, esters of sorbitol dehydrate, carboxylic acid copolymers, esters and ethers of glucose, ethoxylated ethers, ethoxylated alcohols, alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acid amides, acyl lactylates, soaps, DEA oleyl alcohol polyoxyethylene-3 ether phosphates and mixtures thereof.

[0063] One or more thickeners, non-limiting examples include carboxylic acid polymers, cross-linked polyacrylate polymers, polyacrylamide polymers, polysaccharides and glue. Examples of commercially available carboxylic acid polymers include carbomer, which is a homopolymer cross-linked by the allyl ether of acrylic acid and sucrose or pentaerythritol. Non-limiting examples of polysaccharides include cellulose, carboxymethyl hydroxyethyl cellulose, cellulose acetate propionate carboxylate, hydroxyethyl cellulose, hydroxyethyl methyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl hydroxyethyl cellulose, microcrystalline cellulose, sodium cellulose sulfate and mixtures thereof. Non-limiting examples of glue include gum arabic, agar, algin, alginic acid, ammonium alginate, branched starch, calcium alginate, hydrated silicon dioxide, hydroxypropyl chitosan, hydroxypropyl guar gum, karaya gum, giant kelp, carob bean gum, natto gum, potassium alginate, potassium carrageenan, propylene glycol alginate, sclerotium gum, sodium carboxymethyl dextran, sodium carrageenan, tragacanth gum, xanthan gum and mixtures thereof.

[0064] Non-limiting examples of preservatives that can be used in the context of the present invention include quaternary ammonium preservatives (e.g., polyquaternium-73), parabens (e.g., methylparaben), phenoxyethanol, sorbic acid, thimerosal, 1,2 hexanediol, 1,2 pentanediol, and the like polyols, or combinations thereof.

[0065] Non-limiting examples of oils that can be used in the context of the present invention include coriander oil, thyme oil, allspice oil, rose oil, impatiens oil, bergamot oil, cedar oil, chamomile oil, clary sage oil, clary sage oil, clove oil, fennel oil, frankincense oil, geranium oil, meadowfoam seed oil, caprylic / capric triglyceride, or combinations thereof.

[0066] In some embodiments, the cosmetic is a facial cream, and the facial cream includes the following components in percentage by weight: 1-20% anti-aging composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH adjuster, and the balance water.

[0067] For example, in different embodiments, the mass percentage of the thickener may be, but is not limited to, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 1.5%, 2%, 2.5%, or 3%.

[0068] For example, in different embodiments, the mass percentage of the moisturizing agent may be, but is not limited to, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.8%, 0.9%, 0.95%, or 1%.

[0069] For example, in different embodiments, the mass percentage of the emulsifier can be but is not limited to 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%.

[0070] For example, in different embodiments, the mass percentage of the preservative may be, but is not limited to, 0.5%, 1%, 1.5%, 2%, 2.5%, or 3%.

[0071] For example, in different embodiments, the mass percentage of the pH adjuster may be, but is not limited to, 0.01%, 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, or 0.3%.

[0072] In some embodiments, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0073] In some embodiments, the moisturizer includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0074] In some embodiments, the pH adjuster includes at least one of arginine, tromethamine, and EDTA-disodium;

[0075] In some embodiments, the emulsifier includes at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0076] In some embodiments, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0077] In order to further understand the present application, the antioxidant composition of the present application, its preparation method and effect are further described in detail below in conjunction with specific examples. All raw materials involved in the present application can be obtained through commercial purchase.

[0078] The raw materials used in the embodiments and comparative examples are described below, but are not limited to these materials:

[0079] The golden camellia extract-1 is homemade, and its preparation method comprises the following steps:

[0080] Collect the flowers of Camellia chrysantha, clean them, put them into a 50°C constant temperature oven and dry them to constant weight, grind them and pass them through an 80-mesh sieve to obtain Camellia chrysantha powder;

[0081] Take 1g of Camellia chrysantha powder, add 20mL of 70% ethanol by volume, extract by ultrasonic at 40°C for 30min with a power of 120W, centrifuge the product, take the supernatant and concentrate it to 5mL to obtain Camellia chrysantha extract-1.

[0082] Camellia chrysantha extract-2 is homemade, and its preparation method comprises the following steps:

[0083] Collect the flowers of Camellia chrysantha, clean them, put them into a 50°C constant temperature oven and dry them to constant weight, grind them and pass them through an 80-mesh sieve to obtain Camellia chrysantha powder;

[0084] Take 1g of Camellia chrysantha powder, add 30mL of 90% ethanol by volume, and perform ultrasonic extraction at 30°C for 50min with a power of 200W. The obtained product is centrifuged and the supernatant is concentrated to 5mL to obtain Camellia chrysantha extract-2.

[0085] Camellia chrysantha extract-3 is homemade, and its preparation method comprises the following steps:

[0086] Collect the flowers of Camellia chrysantha, clean them, put them into a 50°C constant temperature oven and dry them to constant weight, grind them and pass them through an 80-mesh sieve to obtain Camellia chrysantha powder;

[0087] Take 1g of Camellia chrysantha powder, add 20mL of 70% ethanol by volume, and reflux extract at 80°C for 1h to obtain Camellia chrysantha extract-3.

[0088] Camellia chrysantha extract-4 is homemade, and its preparation method comprises the following steps:

[0089] Collect the flowers of Camellia chrysantha, clean them, put them into a 50°C constant temperature oven and dry them to constant weight, grind them and pass them through an 80-mesh sieve to obtain Camellia chrysantha powder;

[0090] Take 1 g of Camellia chrysantha powder, add 20 mL of 70% ethanol by volume, and perform microwave extraction for 30 minutes at a microwave power of 200 W to obtain Camellia chrysantha extract-4.

[0091] Camellia chrysantha extract-5 is homemade. The preparation method thereof is different from that of Camellia chrysantha extract-1 in that ethyl acetate is used to replace ethanol, and the other components, weight portions, steps and preparation parameters are exactly the same.

[0092] Camellia chrysantha extract-6 is homemade. The only difference between its preparation method and that of Camellia chrysantha extract-1 is that petroleum ether is used to replace ethanol, and the other components, weight parts, steps and preparation parameters are exactly the same.

[0093] Bayberry bark extract-1: The mass percentage of myricitrin is 10%, purchased from Xi'an Four Seasons Biotechnology Co., Ltd.

[0094] Bayberry bark extract-2: The mass percentage of myricitrin is 0.5%, purchased from Shaanxi Huikangyuan Biomedical Technology Co., Ltd.

[0095] Cnidium monnieri extract-1: The mass percentage of osthole is 5%, purchased from Xi'an Shenghecui Biotechnology Co., Ltd.

[0096] Cnidium monnieri extract-2: The mass percentage of osthole is 0.5%, purchased from Shaanxi Jiangu Bioengineering Co., Ltd.

[0097] Camellia extract: purchased from Shaanxi Yiruinong Biotechnology Co., Ltd.

[0098] Aesculus hippocastanum bark extract was purchased from Xi'an Meihe Biotechnology Co., Ltd.

[0099] Cassia seed extract was purchased from Shaanxi Tech Biotechnology Co., Ltd.

[0100] Inositol: purchased from Shanghai Yuanfa Biotechnology Co., Ltd.

[0101] Ceramide-NP: purchased from Guangzhou Snoco Biotechnology Co., Ltd.

[0102] Phytosphingosine: purchased from Shaanxi Peptide Source Biotechnology Co., Ltd.

[0103] Examples 1-13 and Comparative Examples 1-7

[0104] The embodiments and comparative examples of the present invention provide an anti-aging composition specially developed for repairing dry and sensitive skin, and the components of the anti-aging composition are shown in Table 1 and Table 2;

[0105] The preparation method of the anti-aging composition of Examples 1-13 and Comparative Examples 1-7 is as follows: uniformly mix the components according to the weight parts in Table 1 and Table 2 to obtain the anti-aging composition.

[0106] Table 1

[0107]

[0108]

[0109] Table 2

[0110]

[0111] Application examples 1-16, comparative application examples 1-7 and blank application examples

[0112] The application examples, comparative application examples and blank application examples of the present invention provide an anti-aging cream, the components (mass percentage) of the anti-aging cream are shown in Table 3; wherein the anti-aging compositions used in application examples 1-13 are respectively the anti-aging compositions prepared in embodiments 1-13, the anti-aging compositions used in comparative application examples 1-7 are respectively the anti-aging compositions prepared in comparative examples 1-7; the anti-aging compositions used in application examples 14-16 are the anti-aging compositions prepared in embodiment 1;

[0113] The preparation methods of the anti-aging creams of the application examples, comparative application examples and blank application examples are as follows:

[0114] (1) Mixing a humectant, a thickener and water, heating to 85° C., homogenizing at a speed of 1200 rpm for 4 minutes, and keeping the mixture warm for later use to obtain a prefabricated component A;

[0115] (2) mixing the emulsifier, heating to 85° C., homogenizing at 1200 rpm for 4 min, and keeping warm for later use to obtain prefabricated component B;

[0116] (3) mixing the preservatives and heating to 60° C. to melt to obtain a prefabricated C component;

[0117] (4) Heat the preformed component A to 80°C, add the preformed component B at a speed of 250 rpm, stir and mix evenly, then cool to 60°C, add the preformed component C at a speed of 250 rpm, stir and mix, then cool to below 40°C, add the anti-aging composition and continue stirring for 5 minutes, finally add a pH adjuster to adjust the pH to 6.0, stop stirring, discharge, and obtain the anti-aging cream.

[0118] Table 3

[0119]

[0120]

[0121] Effect Example 1 Penetration-enhancing and Repairing Effect Test

[0122] This effect example explores the penetration-enhancing effect and repairing effect of the anti-aging composition prepared by Examples 1-13 and Comparative Examples 1-7.

[0123] Experimental materials: The cell line used was human keratinocyte HaCaT (Beina Biotechnology).

[0124] The specific test method is as follows:

[0125] (1) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of culture medium was added to each well. After culturing for 24 hours, the supernatant was discarded.

[0126] (2) The blank control group was added with 100 μL of DMEM medium (2105341, Gibco), and the sample group was added with 100 μL of DMEM medium (2105341, Gibco) and 0.01% of the anti-aging composition, and incubated at 37°C, 5% CO 2 After incubation in the incubator for 5 h and 24 h, the cells were collected and washed three times with PBS buffer. 50 μL of RIPA cell lysis buffer containing protease inhibitors was added to each well. The lysed samples were centrifuged at 12000 g for 5 min, and the supernatant was collected.

[0127] (3) Determination of tight junction protein Claudin-1 content: The Claudin-1 protein content in the cell supernatant of each group was detected using a human Claudin-1 ELISA kit (Cat. No. BLL106520E, Baililai Biotechnology).

[0128] Tight junctions are the main mode of cell connection and regulate the permeability of the skin barrier. The lower the expression level of tight junction protein Claudin-1 after 5 hours of sample treatment, the higher the permeability of the skin barrier, and the better the penetration-enhancing effect of the anti-aging composition; the higher the expression level of tight junction protein Claudin-1 after 24 hours of sample treatment, the lower the permeability of the skin barrier, the better the effect of isolating external irritants from entering the skin, and the better the repair effect of the anti-aging composition. The penetration-enhancing effect of the anti-aging composition is represented by the reduction rate of Claudin-1 in 5 hours, and the repair effect of the anti-aging composition is represented by the increase rate of Claudin-1 in 24 hours. The formula is as follows:

[0129] 5-hour Claudin-1 reduction rate = 1-Claudin-1 content in sample group / Claudin-1 content in blank group*100%;

[0130] 24-hour Claudin-1 increase rate = (Claudin-1 content in sample group / Claudin-1 content in blank group - 1) * 100%;

[0131] The test results are shown in Table 4.

[0132] Table 4

[0133]

[0134] From the test results in Table 4, it can be seen that after applying the anti-aging composition of Examples 1-13 provided by the present invention, the Claudin-1 reduction rate is 30.5-54.6% in 5 hours, and the Claudin-1 increase rate is 51.4-79.1% in 24 hours, indicating that the anti-aging composition of the present invention has good penetration-enhancing effect and repairing effect.

[0135] By comparing Example 1 with Comparative Examples 1-7, it can be seen that when any one of Camellia chrysantha extract, Bayberry bark extract, and Cnidium monnieri extract is missing or replaced by other functional analogs, the Claudin-1 reduction rate in 5 hours is less than 13%, and the Claudin-1 increase rate in 24 hours is less than 25%, indicating that the lack of any one of Camellia chrysantha extract, Bayberry bark extract, and Cnidium monnieri extract or the use of other functional analogs to replace them will lead to a decrease in the penetration-promoting and repairing effects of the anti-aging composition.

[0136] Effect Example 2 Anti-aging Effect Test

[0137] This effect example explores the anti-aging effects of the anti-aging compositions prepared in Examples 1-13 and Comparative Examples 1-7.

[0138] Experimental materials: The cell line used was human dermal fibroblast HDF (Cat. No. PC-202h, Wuhan Saios Biotechnology Co., Ltd.), β-galactosidase activity assay kit (Cat. No. ab287846, abcam).

[0139] The specific test method is as follows:

[0140] (1) Inoculation: The cells were inoculated into culture flasks at a culture density of 1×10 6 The cells were cultured in DMEM high glucose medium (Gibco) containing 10% fetal bovine serum at 37°C and 5% CO 2 After 24h incubation in the incubator;

[0141] (2) Induction: Treat with PBS buffer containing 50 μM hydrogen peroxide for 30 min, discard the PBS containing hydrogen peroxide, and then wash with PBS three times;

[0142] (3) Sample administration: The blank control group was added with DMEM high-glucose culture medium containing 10% fetal bovine serum, and the sample group was added with DMEM high-glucose culture medium containing 10% fetal bovine serum and 0.01% anti-aging composition. The cells were incubated at 37°C and 5% CO 2 After continuing to culture in the incubator for 24 h, the supernatant was collected;

[0143] (4) β-galactosidase activity assay: A β-galactosidase activity assay kit (Cat. No. ab287846, abcam) was used to detect the cellular senescence level.

[0144] Hydrogen peroxide can induce cell senescence, which is marked by an increase in β-galactosidase activity. Therefore, after sample treatment, the lower the β-galactosidase activity, the lower the level of cell senescence, and the stronger the anti-aging ability of the repair and anti-aging composition. The anti-aging ability of the repair and anti-aging composition is represented by the decrease in β-galactosidase activity, and the calculation formula is as follows:

[0145] Cell senescence improvement rate = 1-β-galactosidase activity 样品组 / β-galactosidase activity 对照组 *100%;

[0146] The test results are shown in Table 5.

[0147] Table 5

[0148]

[0149]

[0150] From the test results in Table 5, it can be seen that after applying the anti-aging composition of Examples 1-13 provided by the present invention, the cell aging improvement rate is 54.1-81.9%, indicating that the anti-aging composition of the present invention has a good anti-aging effect.

[0151] By comparing Example 1 and Comparative Examples 1-7, it can be seen that the cell aging improvement rate is lower than 26% when any one of Camellia chrysantha extract, Bayberry bark extract and Cnidium monnieri extract is lacking or other functional analogs are used for replacement, indicating that the anti-aging effect of the anti-aging composition will be reduced when any one of Camellia chrysantha extract, Bayberry bark extract and Cnidium monnieri extract is lacking or other functional analogs are used for replacement.

[0152] Effect Example 3 Human Skin Patch Test

[0153] This effect example explores the irritation of the anti-aging cream prepared by Application Examples 1-16 and Comparative Application Examples 1-7 to the skin.

[0154] 30 volunteers were recruited, 15 males and 15 females, aged 20-50 years old. The closed patch test method was used. An equal amount (0.020mL-0.025mL) of the test sample was placed in a specific patch tester, which was applied to the volunteer's arm with a hypoallergenic tape, and gently pressed to evenly apply it on the skin for 24 hours; the blank control group was distilled water, and the negative control group was the blank anti-aging cream of the application example. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours, and 48 hours, and the results were recorded. The level of adverse reactions to the skin is shown in Table 6 below.

[0155] Table 6

[0156]

[0157] The anti-aging creams provided in Application Examples 1-16 of the present invention and Comparative Application Examples 1-7 all showed negative reactions after human patch tests, and they are safe and non-irritating to human skin, indicating that the anti-aging composition of the present invention has low irritation.

[0158] Effect Example 4 Human Efficacy Test

[0159] This effect example tests the improvement effects of application examples 1-16, comparative application examples 1-7 and blank application examples on lipidomics, repair, moisturizing, wrinkle reduction and soothing of dry sensitive skin.

[0160] The specific test method is as follows:

[0161] Through lipidomics research on sensitive skin, the inventors found that dry sensitive skin, compared with healthy skin and oily sensitive skin, lacks three lipid components, namely lysophosphatidylinositol, ceramide-NP and sphingosine, which leads to damaged skin lipid barrier, dry skin, redness and susceptibility to fine lines. Therefore, after volunteers used the repair and anti-aging composition, the higher the content of these three lipid components, the better the effect of the composition in repairing the skin lipid barrier.

[0162] According to the "Technical Specifications for Safety of Cosmetics" (2015), 120 Asian adult testers aged 45-60 with dry and sensitive skin and wrinkles at the corners of the eyes were selected and randomly divided into 24 groups, with 5 people in each group. Under normal circumstances, the volunteers continued to use the product on their entire face for 28 days (T28); every morning and evening, after cleansing and moisturizing, the volunteers took an appropriate amount of anti-aging cream and applied it to the face, massaged it for absorption, and used it on the entire face once in the morning and evening. The data were collected before use and 28 days after use. On the day of the volunteer's visit, the test area washed its face with clean water without applying any product, and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 2 hours. Skin tape D-SquameTM D100 was used to collect sebum from the forehead and cheeks of volunteers for 5 times. After the samples were collected, quantitative lipidome sequencing analysis was performed using the quantitative lipidome sequencer of Wuhan Maiwei Metabolic Biotechnology Co., Ltd. According to the sequencing results, the average contents of lysophosphatidylinositol, ceramide-NP and sphingosine in the skin of the volunteers were counted, and the corresponding content increase rate was calculated. The formula is as follows: lipid increase rate = (T28 样品组 -T0 样品组 ) / T0 样品组 *100%]-[(T28 空白应用例 -T0 空白应用例 ) / T0 空白应用例 *100%].

[0163] At the same time, the skin moisture loss test probe TewaMeter was used to measure the transepidermal water loss value TEWL of the zygomatic bone, the skin moisture content of the zygomatic bone was measured using the skin moisture test probe Corneometer, the facial wrinkles were photographed and analyzed using VISIA-CR, and the skin discomfort of the volunteers was evaluated using a 10-point self-assessment questionnaire (the higher the score, the stronger the discomfort).

[0164] The skin barrier repair ability is represented by the improvement of TEWL value, the moisturizing effect is represented by the improvement of skin moisture content, the wrinkle reduction effect is represented by the improvement of wrinkle area at the end of the eye, and the soothing effect is represented by the improvement of skin discomfort self-assessment score. The formula is as follows:

[0165] TEWL value improvement rate = (T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用

[0166] 例 ) / T0 空白应用例 *100%];

[0167] Skin moisture content improvement rate = (T28 样品组 -T0样品组 ) / T0 样品组 *100%]-[(T28 空白应用例 -T0 空白应用例 ) / T0 空白应用例 *100%];

[0168] Improvement rate of eye wrinkles = (T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用例 ) / T0 空白应用例 *100%];

[0169] Skin discomfort improvement rate = (T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用例 ) / T0 空白应用例 *100%].

[0170] The results are shown in Table 7.

[0171] Table 7

[0172]

[0173]

[0174] From the experimental results in Table 7, it can be seen that when the anti-aging cream of Application Examples 1-16 of the present invention is used, the increase rate of lysophosphatidylinositol in dry and sensitive skin is 35.8-89.3%, the increase rate of ceramide-NP is 17.2-37.7%, the increase rate of sphingosine is 23.4-44.9%, the improvement rate of TEWL value is 25.3-57.2%, the improvement rate of skin moisture content is 21.6-41.3%, the improvement rate of wrinkles at the end of the eyes is 12.6-23%, and the improvement rate of skin discomfort is 54.8-85.5%; it shows that the application of the anti-aging composition of the present invention can effectively increase lysophosphatidylinositol, ceramide-NP and sphingosine in dry and sensitive skin, and improve the TEWL, moisture content, wrinkles at the end of the eyes and discomfort of the skin, that is, the anti-aging composition of the present invention has good repairing, moisturizing, anti-aging and soothing effects.

[0175] It can be seen from Application Example 1 and Comparative Application Examples 1-7 that the lack of any one of Camellia chrysantha extract, Bayberry bark extract, and Cnidium monnieri extract or the use of other functional analogs for replacement, the increase rate of lysophosphatidylinositol in dry and sensitive skin is lower than 32%, the increase rate of ceramide-NP is lower than 10%, the increase rate of sphingosine is lower than 9.5%, the improvement rate of TEWL value is lower than 8%, the improvement rate of skin moisture content is lower than 9%, the improvement rate of wrinkles at the tail of the eye is lower than 6%, and the improvement rate of skin discomfort is lower than 14.5%, indicating that the lack of any one of Camellia chrysantha extract, Bayberry bark extract, and Cnidium monnieri extract or the use of other functional analogs for replacement will lead to a decrease in the anti-aging effect of the anti-aging composition.

[0176] Finally, it should be noted that the above embodiments are used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-aging composition, characterized in that: The anti-aging composition comprises the following components in parts by weight: 0.1-3 parts of inositol, 0.01-1 parts of ceramide-NP, 0.01-1 parts of phytosphingosine, 0.1-5 parts of golden camellia extract, 0.1-5 parts of bayberry bark extract, and 0.1-5 parts of cnidium monnieri extract.

2. The anti-aging composition according to claim 1, characterized in that The anti-aging composition comprises the following components in parts by weight: 0.5-2 parts of inositol, 0.05-0.5 parts of ceramide-NP, 0.05-0.5 parts of phytosphingosine, 0.5-3 parts of golden camellia extract, 0.5-3 parts of bayberry bark extract, and 1-4 parts of cnidium monnieri extract.

3. The anti-aging composition according to claim 2, characterized in that: The anti-aging composition comprises the following components in parts by weight: 1-1.5 parts of inositol, 0.1-0.2 parts of ceramide-NP, 0.2-0.3 parts of phytosphingosine, 1-2 parts of golden camellia extract, 1-2 parts of bayberry bark extract, and 2-3 parts of cnidium monnieri extract.

4. The anti-aging composition according to claim 1, characterized in that In the anti-aging composition, the mass percentage of the Camellia chrysantha extract is 14-30%; and / or the mass percentage of myricitrin in the Bayberry bark extract is 5-15%; and / or the mass percentage of osthole in the Cnidium monnieri extract is 2-10%.

5. The anti-aging composition according to claim 1, characterized in that The preparation method of the Camellia chrysantha extract comprises the following steps: The flowers of Camellia chrysantha are dried, crushed, and passed through a 80-150 mesh sieve to obtain Camellia chrysantha powder; The camellia chrysantha powder is added into an ethanol solution for extraction and concentrated into a paste to obtain the camellia chrysantha extract; the solid-liquid ratio of the camellia chrysantha powder to the ethanol solution is 1g:10-30mL.

6. The anti-aging composition according to claim 5, characterized in that The volume fraction of the ethanol solution is 60-90%; and / or, the extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 30-40° C., the time of ultrasonic extraction is 20-50 min, and the power of ultrasonic extraction is 80-200W.

7. Use of the anti-aging composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. A cosmetic, characterized in that: The cosmetic comprises the anti-aging composition according to any one of claims 1 to 6, wherein the content of the anti-aging composition in the cosmetic is 1 to 20 wt %.

9. The cosmetic according to claim 8, characterized in that The cosmetic is a facial cream, and the facial cream comprises the following components in percentage by mass: 1-20% anti-aging composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH regulator, and the balance water.

10. The cosmetic according to claim 9, characterized in that The thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; And / or, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide; And / or, the pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium; And / or, the emulsifier includes at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; And / or, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

Images