Preservative composition for food products

Abstract

The present invention relates to a preservative composition comprising at least three compounds selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol, and methyl salicylate, and also to preserved food products containing such a preservative composition.

Description

[Technical field]

[0001] The present invention relates to a preservative composition and to preserved food products comprising said preservative composition. [Background technology]

[0002] Spoilage caused by various microorganisms is one of the causes of food waste. Spoilage is the process by which food deteriorates, reducing its suitability for consumption and ultimately rendering it inedible to humans.

[0003] Molds and / or yeasts growing on or within a food matrix can cause food spoilage. Bacteria can also cause food spoilage. During the process of bacterial breakdown of food, acids and metabolic products are usually produced, and even if the bacteria themselves are not harmful, the waste products can be unpleasant to taste or even harmful to health.

[0004] Consumers increasingly want their convenience foods to remain fresh and have a long shelf life. The addition of preservatives to food products (e.g. beverages, spreads, dressings, convenience foods, etc.) is a common practice in the food industry. The market for preservatives is expanding in response to consumer demand.

[0005] Many countries have regulations that prohibit the use of certain food additives, including some preservatives, in foods and beverages. Although such regulations can vary widely, there is a clear trend toward fewer and lower levels of chemical preservatives, especially synthetic chemical preservatives, in foods.

[0006] Preservatives frequently used in food products include sorbates and benzoates. Unfortunately, the use of such preservatives often can impair the flavor of certain food products. Furthermore, some consumers consider these preservatives to be a type of chemical additive that should be avoided. In fact, there is a growing consumer trend toward so-called "clean label" food products.

[0007] However, replacing existing preservatives with "clean label" alternatives is difficult. In particular, replacing sorbates is a challenge, as many of the "natural" alternatives do not have sufficient antifungal activity. Summary of the Invention [Problem to be solved by the invention]

[0008] Thus, there is a need for "clean label" preservative compositions that would provide the beneficial qualities of sorbates, particularly in terms of antifungal activity. Similarly, food formulations that contain low levels of synthetic preservatives would be desirable. [Means for solving the problem]

[0009] The inventors have identified that certain combinations of volatile aroma compounds have antimicrobial activity.Accordingly, in a first aspect, the present invention provides a preservative composition comprising at least three compounds selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate.

[0010] The invention also provides a preserved foodstuff comprising the preservative composition, and a method for preparing the preserved foodstuff comprising the step of adding the preservative composition to a foodstuff susceptible to microbial spoilage.

[0011] Finally, the present invention relates to the use of a composition comprising at least three compounds selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate as a preservative, in particular in such a use where the composition reduces or prevents the growth of one or more fungal spoilage microorganisms in a foodstuff. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The present invention relates to a preservative composition comprising at least three compounds, preferably at least four compounds, more preferably at least five compounds, selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate, and also relates to a preserved food product comprising such a preservative composition.Preferably, the preservative composition comprises E-2-hexenal and at least two compounds, more preferably at least three compounds, even more preferably at least four compounds, selected from hexanal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate.

[0013] The preservative composition of the present invention can be composed of hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate.Similarly, the preserved food product can include a preservative system composed of hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate.

[0014] A good preservative composition inhibits spoilage microorganisms without interfering with the sensory properties of the foodstuff. Preserved foodstuffs preferably contain 10 ppm to 10,000 ppm of the preservative composition, more preferably 50 ppm to 5,000 ppm, and most preferably 100 ppm to 2,500 ppm. The concentrations stated relate to the level of the preservative composition present in the final product to be stored (i.e., the level found in the preserved foodstuff). It should be noted that this does not necessarily translate to the level in the foodstuff to be consumed, since some foodstuffs are sold in concentrated form and will be diluted before consumption. Thus, a foodstuff to be consumed may have a lower concentration of the preservative composition than a foodstuff to be stored.

[0015] The inventors have found that the presence of linalool in preserved foodstuffs tends to suppress the effectiveness of preservative composition.Therefore, it is preferred that preserved foodstuffs contain less than 60 ppm linalool, more preferably less than 30 ppm linalool, and even more preferably less than 15 ppm linalool.It is particularly preferred that preserved foodstuffs are substantially free of linalool.Once again, these concentrations refer to the levels in the preserved foodstuffs that are stored.

[0016] Some of the more commonly used preservatives in formulating food products that are protected from spoilage are sorbates. As used herein, the term "sorbate" includes sorbic acid (E200) and its salts, including sodium sorbate (E201), potassium sorbate (E202) and calcium sorbate (E203). Sorbates are particularly effective antifungal agents, and finding a "natural" substitute has been a challenge. The inventors have found that the preservative compositions of the present invention can be used to completely or partially replace sorbates in food products. For example, such preservative compositions may allow reduced levels of sorbates to be used in food products (while still achieving the same anti-spoilage effect as conventional amounts of sorbates). A typical amount of potassium sorbate in food products is 250 ppm to 1000 ppm. Thus, foodstuffs according to the present invention preferably contain less than 200 ppm sorbate, more preferably less than 100 ppm sorbate, even more preferably less than 50 ppm sorbate, and most preferably less than 10 ppm sorbate.

[0017] Benzoates represent another type of commonly used preservative, especially in acidic foods such as salad dressings and soft drinks. The inventors have found that the preservative composition of the present invention can be used to completely or partially replace benzoates in foodstuffs. For example, the preservative composition may allow the use of reduced levels of benzoates in foodstuffs (while still achieving the same anti-spoilage effect as conventional amounts of sorbates). As used herein, the term "benzoate" includes benzoic acid (E210) and its salts, including sodium benzoate (E211), potassium benzoate (E212) and calcium benzoate (E213). Typical amounts of sodium benzoate in foodstuffs are 150 ppm to 1000 ppm. Thus, foodstuffs according to the present invention preferably contain less than 500 ppm benzoate, more preferably less than 100 ppm benzoate, even more preferably less than 50 ppm benzoate, and most preferably less than 10 ppm benzoate.

[0018] The foodstuff of the present invention is a foodstuff that is susceptible to spoilage by spoilage, preferably selected from the group consisting of beverages (including non-carbonated or carbonated soft drinks, fruit drinks, beverage concentrates, multiserve cordials, energy drinks, flavoured waters, nectars, sports drinks), sauces, dressings, marinades, ketchups, condiments, bouillons, soups, desserts, confectionery products and ice creams.

[0019] The preserved foodstuffs are preferably packaged. Examples of suitable packaging containers include, but are not limited to, bottles, cans, cartons, pouches, and sachets. The preserved foodstuffs are preferably sanitized, for example by pasteurization or sterilization.

[0020] Examples of food products in which the preservative composition may be included include packaged ready-to-drink beverages or packaged beverage concentrates. The preservative composition is particularly suitable for incorporation into tea-based beverages. However, other beverages, including fruit drinks and soft drinks, may also be stabilized using the preservative composition. The beverage may be non-carbonated or carbonated.

[0021] If the beverage is a tea-based beverage, it will contain tea solids. The tea solids may be provided by any suitable source, such as tea extract (preferably in powder form), compressed tea juice, etc. The skilled artisan will know how to obtain such ingredients.

[0022] Tea is known to have certain antimicrobial properties in itself, but these properties (i.e., inhibition of yeast and mold growth) are only evident at tea solids concentrations above 3%. At lower concentrations, typical for tea-based beverages (including tea concentrates), tea acts as a nutrient that increases the likelihood of microbial spoilage. The beverage preferably comprises tea solids at 0.01% to 3%, more preferably 0.05% to 3%, and most preferably 0.1% to 2%, by weight of the beverage. As used herein, the term "tea solids" refers to the dried material obtained from the leaves of the plants Camellia sinensis var. sinensis and / or Camellia sinensis var. assamica.

[0023] As used herein, the term "beverage" refers to a substantially aqueous composition. The beverage may be in any form, for example, ready-to-drink or concentrated. "Ready-to-drink beverage" refers to a drinkable composition suitable for human consumption, preferably comprising at least 85% by weight water, more preferably at least 90% by weight water, and most preferably at least 95% by weight water.

[0024] "Beverage concentrate" means a beverage composition that requires dilution, typically with an aqueous liquid (e.g., water or milk) prior to consumption, and thus this form will typically have a higher solids content (and therefore a lower water content) than a ready-to-drink form. For example, prior to dilution, a beverage concentrate preferably comprises at least 25% to 85% by weight water, more preferably 40% to 80% by weight water, and most preferably at least 50% to 75% by weight water.

[0025] The preserved beverage preferably has an acidic pH (i.e. a pH less than 7). In particular, the pH (at 20° C.) is preferably less than 5, more preferably less than 4.5, and most preferably between 2 and 4.

[0026] To achieve an acidic pH, the preserved beverage preferably comprises one or more acidulants. Suitable acidulants include organic acids such as citric acid, malic acid, lactic acid, tartaric acid, ascorbic acid, phosphoric acid, and their salts. Mixtures of one or more of these acidulants are also suitable. A particularly well-balanced flavor can be provided when the acidulant comprises citric acid and / or its salts. Mixtures of citric acid (and / or its salts), malic acid (and / or its salts) and ascorbic acid (and / or its salts) also provide good flavor. Typically, the concentration of the acidulant in the preserved beverage will be between 0.001 and 1% by weight, more preferably between 0.01 and 0.5% by weight.

[0027] It is particularly preferred that the preserved beverage is a flavored beverage, more preferably a fruit flavored beverage, most preferably a fruit flavored tea beverage.Suitable flavors include natural or synthetic fruit flavors and / or natural or synthetic herbal flavors.Examples of fruit flavors include apple, apricot, blackcurrant, cherry, cranberry, grape, grapefruit, guava, kiwi, lemon, lime, lychee, mandarin, mango, nectarine, orange, peach, pear, pineapple, plum, passion fruit, raspberry and strawberry.Examples of herbal flavors include chamomile, chrysanthemum, elderflower, hawthorn, hibiscus, jasmine, yerba mate, mint (e.g., peppermint, spearmint), osmanthus, rose and verbena (e.g., lemon verbena).

[0028] Consumers prefer sweet tasting beverages, therefore the preserved beverages preferably contain a nutritive sweetener, a non-nutritive sweetener, or a mixture thereof.

[0029] Non-nutritive sweeteners allow beverages to be formulated so that they still have a pleasant sweet taste despite their low energy content. Health-conscious consumers often prefer such beverages. Preferred examples of non-nutritive sweeteners include aspartame, saccharin, acesulfame K, glycyrrhizin, stevia-derived sweeteners (e.g., stevioside, rebaudioside A, rebaudioside C, dulcoside A, etc., with preferred examples being stevioside and / or rebaudioside), sucralose, and mixtures thereof. The most preferred non-nutritive sweeteners are acesulfame K, aspartame, sucralose, rebaudioside A, or mixtures thereof, due to their well-balanced flavor. The concentration of the non-nutritive sweetener will depend on the relative sweetness of the sweetener and the composition of the beverage. Typically, the preserved beverage will contain non-nutritive sweeteners in an amount of from 0.00001 to 10%, more preferably from 0.001 to 1%, and most preferably from 0.01 to 0.1% by weight of the beverage.

[0030] On the other hand, consumers may prefer the perceived naturalness of nutritive sweeteners. Examples of nutritive sweeteners include glucose, sucrose, fructose and mixtures thereof. An example of a particularly preferred natural nutritive sweetener is honey.

[0031] Preserved beverages may have a high calorie content (e.g., an energy content of more than 100 kCal, preferably between 150 and 1000 kCal per 100 g of beverage). Such beverages preferably contain one or more nutritive sweeteners, optionally in combination with one or more non-nutritive sweeteners.

[0032] In a preferred embodiment, the preserved beverage is a low-calorie beverage (e.g., has an energy content of less than 100 kCal per 100 g of beverage). It is particularly preferred that a serving of the preserved beverage has a total energy content of less than 10 kCal, more preferably less than 5 kCal, and most preferably less than 1 kCal. The low-calorie beverage preferably contains one or more non-nutritive sweeteners.

[0033] Preserved foodstuffs can be prepared in any convenient way, but preferably by the method according to the present invention.As presented above, one aspect of the present invention relates to the method for preparing preserved foodstuffs, comprising adding to foodstuffs susceptible to microbial spoilage an antiseptic composition comprising at least three compounds selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate.Preferably, the foodstuffs susceptible to microbial spoilage are beverages.

[0034] Preferably, the preservative composition is added to the foodstuff in an amount such that the foodstuff contains from 10 ppm to 10,000 ppm, more preferably from 50 ppm to 5,000 ppm, and most preferably from 100 ppm to 2,500 ppm of the preservative composition.

[0035] The present method is preferably used for preparing the above-mentioned preserved foodstuffs, therefore the preferred technical features described for the preserved foodstuffs also apply mutatis mutandis to the present method.

[0036] Finally, the present invention relates to the use of the composition comprising at least three compounds selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate as preservative.For example, the use of such composition is to reduce or prevent the growth of one or more fungal spoilage microorganisms in foodstuffs, preferably beverages.

[0037] As used herein, the term "comprising" encompasses the terms "consisting essentially of" and "consisting of." When the term "comprising" is used, the listed steps or options need not be exhaustive. Except in the examples and comparative experiments, or unless otherwise expressly indicated, all numerical values ​​should be understood to be modified by the word "about." As used herein, the indefinite article "a" or "an" and its corresponding definite article "the" mean at least one, or one or more, unless otherwise expressly indicated.

[0038] Unless otherwise indicated, numerical ranges expressed in the format "from x to y" are understood to include x and y. When specifying any range of values ​​and amounts, any particular upper value or amount may be associated with any particular lower value or amount. All percentages and ratios contained herein are calculated by weight unless otherwise indicated.

[0039] Various features of the invention referred to in individual sections above apply mutatis mutandis to other sections, as appropriate. Thus, features specified in one section may be combined with features specified in other sections, as appropriate. Any section headings are added for convenience only and are not intended to limit the disclosure in any way.

[0040] The following examples are intended to illustrate the invention and are not intended to limit the invention to those examples per se. EXAMPLES

[0041] Combinations of fragrance molecules were tested for growth inhibition of several common fungal spoilage microorganisms.

[0042] Cold-filled non-preserved peach flavoured tea concentrate was used as the medium, containing black tea extract powder (8.4 g / L), flavour (5.04 g / L), sucrose (444 g / L), citric acid (11.7 g / L), ascorbic acid (1.2 g / L) and water (balance).

[0043] The media was spiked with either an inoculum consisting of a cell suspension of two yeasts (Candida parapsilosis and Zygosaccharomyces bailii) or two molds (Paecilomyces variotti and Neosartorya fischeri). In both cases, the media was spiked at a level of approximately 1000 cfu / ml. For each time point, all samples and controls were prepared in triplicate.

[0044] Samples were incubated at 25°C and incubation times ranged from 1 to 12 weeks. At each time point, serial dilutions of each sample and control were plated on OMEA plates. The dilutions were at the MRD and a minimum of 3 dilutions were plated for each sample and control at each time point. Plates were incubated at 25°C for 3 to 5 days and visually inspected for growth of spoilage organisms.

[0045] Example 1 Fragrance composition stock solutions were prepared. Table 1 summarizes the fragrance compounds present in four such fragrance composition stock solutions (samples 1 to 5). The concentrations of compounds (if present) in each fragrance composition stock solution were as follows: methanol (12900 ppm), E-2-hexenal (6280 ppm), linalool (3170 ppm), Z-3-hexenol (1070 ppm), E-linalool oxide (973 ppm), methyl salicylate (833 ppm), hexanal (509 ppm), E-2-hexenol (492 ppm), acetaldehyde (365 ppm), Z-2-penten-1-ol (344 ppm), 1-penten-3-ol (251 ppm), 1-penten-3-one (107 ppm).

[0046] For fungal growth inhibition tests, the appropriate stock solution was diluted in medium (i.e., unpreserved peach flavored tea concentrate) so that the medium contained 2% (by volume) of the aroma composition of interest. Appropriate controls were included with each test. These controls were as follows:

[0047] Control 1: Positive control (i.e., medium spiked with inoculum and not containing any aroma composition); Control 2: Negative control (i.e., non-spiked medium containing 2% of the aroma composition), and Control 3: Sterile control (i.e., non-spiked medium without any aroma composition).

[0048] [Table 1]

[0049] The results of the fungal growth inhibition tests are summarized in Table 2. Data are from 1:10 serial dilutions and are the average of triplicate samples.

[0050] Regarding controls, the positive control (Control 1) had results >300 cfu / ml at all time points regardless of the inoculum used. Both the negative control (Control 2) and the sterile control (Control 3) had results <1 cfu / ml at all time points (data not included in Table 2).

[0051] All six samples containing hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate (i.e., samples 1, 2, 4 and 5) showed at least some antifungal activity against both yeast and mold, whereas sample 3 (linalool only) showed no antifungal activity against yeast or mold.

[0052] It can be seen that the samples containing the six compounds listed and linalool (i.e., samples 1 and 5) are less effective as long-term antifungals, being most effective for periods of less than one month. In contrast, the samples containing the six compounds listed but not linalool (i.e., samples 2 and 4) show long-term antifungal activity, with sample 4 showing antifungal activity against both yeasts and molds for at least 12 weeks.

[0053] [Table 2]

[0054] Example 2 The antifungal properties of the six compounds in sample 5 were further investigated. Table 3 summarizes 15 additional fragrance composition stock solutions (samples A to O) that contain one or two of these compounds. The concentrations of the compounds (if present) in each fragrance composition stock solution were as follows: methanol (12900 ppm), E-2-hexenal (6280 ppm), E-linalool oxide (973 ppm), methyl salicylate (833 ppm), hexanal (509 ppm), E-2-hexenol (492 ppm).

[0055] [Table 3]

[0056] Again, the appropriate stock solution was diluted in medium (i.e., unpreserved peach flavored tea concentrate) such that the medium contained 2% (by volume) of the aroma composition of interest. A yeast inoculum was used in this test. Appropriate controls (positive, negative and sterile controls) were included. The positive control resulted in >300 cfu / ml at all time points, while both the negative and sterile controls resulted in <1 cfu / ml at all time points.

[0057] All samples containing only one compound (i.e., samples A, G, and L) had results >300 cfu / ml even after one week. Among the samples containing two compounds, only samples E, I, J, and K showed any appreciable antifungal activity after one week. None of the samples containing two compounds showed significant antifungal activity after two weeks. It was therefore hypothesized that the presence of at least three compounds is required for antifungal activity.

[0058] Example 3 The antifungal properties of additional combinations of compounds in sample 5 were investigated. Table 4 summarizes three additional fragrance composition stock solutions (samples 6 to 8) each containing three of these compounds. The concentrations of the compounds (if present) in each fragrance composition stock solution were as follows: E-2-hexenal (628000 ppm), E-linalool oxide (973 ppm), methyl salicylate (833 ppm), hexanal (509 ppm).

[0059] [Table 4]

[0060] Again, the appropriate stock solution was diluted in medium (i.e., non-preserved peach flavored tea concentrate) such that the medium contained 2% (by volume) of the aroma composition of interest. The medium was spiked with either a yeast inoculum or a mold inoculum. Appropriate controls (positive, negative and sterile controls) were included. Regardless of the inoculum used, the positive control resulted in >300 cfu / ml at all time points, while both the negative and sterile controls resulted in <10 cfu / ml at all time points.

[0061] Samples 6 to 8 all demonstrated antifungal activity (i.e., <10 cfu / ml) after 6 weeks, confirming the hypothesis that antifungal activity requires the presence of at least three compounds.

Claims

1. 1. A method for preparing a preserved food product, comprising the step of adding a preservative composition consisting of at least three compounds selected from hexanal, E-2-hexenal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate to a food product susceptible to microbial spoilage, wherein the preserved food product contains less than 60 ppm linalool.

2. A method for preparing a preserved food product, comprising the step of adding a preservative composition consisting of E-2-hexenal and at least two compounds selected from hexanal, E-2-hexenol, E-linalool oxide, methanol and methyl salicylate to a food product susceptible to microbial spoilage, wherein the preserved food product contains less than 60 ppm linalool.

3. 3. The method of claim 1 or 2, wherein the preservative composition is added to the foodstuff in an amount such that the foodstuff contains from 10 ppm to 10,000 ppm of the preservative composition.

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