Anti-wrinkle compositions containing fully human collagen and uses thereof

Abstract

To provide an anti-wrinkle composition containing fully human collagen, which contains the fully human collagen in combination with an amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyrylbenzylamide diacetate, and applications for the anti-wrinkle composition.SOLUTION: A composition according to the present invention enhances the anti-wrinkle effect of products through the synergistic cooperation of active ingredients such as fully human collagen, an amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyrylbenzylamide diacetate. Particularly, the fully human collagen optimizes the nucleotide sequence of the collagen to enable the preparation of high-purity collagen type I and III in escherichia coli cells, thereby achieving further improvement in the anti-wrinkle effect of the composition. The anti-wrinkle composition containing the fully human collagen is mild, non-irritant, good in stability, very suitable for skin care products and excellent in practicability.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention belongs to the technical field of cosmetics, and specifically relates to a fully human collagen-containing anti-wrinkle composition and its use. [Background technology]

[0002] As we age, the skin inevitably begins to show signs of aging, which in turn leads to wrinkles on the skin and sagging of the face. The above phenomenon is the result of the cooperation of intrinsic and extrinsic factors. Intrinsic aging refers to the progressive increase in AGEs with age, which causes collagen to form intermolecular cross-links, reducing the elasticity and permeability of the skin and weakening the diffusion ability of nutrients and waste products, as well as reducing the solubility of collagen, making it difficult to be hydrolyzed by collagenase, resulting in a decrease in skin elasticity and wrinkles that are difficult to recover from and continue to deepen. Extrinsic aging is caused by photoaging due to sunlight, which causes the skin to become loose and wrinkles to appear. However, when the above problems appear on the skin, it visually makes us feel old, so anti-wrinkle and anti-aging have become the eternal keynote of skin care.

[0003] Patent Document 1 provides an anti-wrinkle and anti-aging essence and its preparation method. The anti-wrinkle and anti-aging essence includes resurrection grass extract, koikema extract, carnosine and base material. The preparation method includes preparing resurrection grass extract and mixing resurrection grass extract, Geshanxiao extract, carnosine and base material. The present invention safely realizes the anti-wrinkle and anti-aging effect of the essence by extracting active ingredients from specific plants and using them in the essence, and through the effective combination of specific plants, it essentially realizes anti-wrinkle and anti-aging by nourishing the skin itself, and fundamentally improves skin quality.

[0004] Patent Document 2 provides an anti-wrinkle and anti-aging cosmetic product and its preparation method. The cosmetic product contains an emulsifier, a moisturizer, an emollient, an active, and water. The skin care composition has a synergistic effect on improving skin elasticity, improves skin elasticity, and can reduce fine lines on the face, reduce sagging, loosening, and wrinkles of the skin, while having the characteristics of light texture and easy absorption.

[0005] However, the above-mentioned products generally have problems such as poor permeability and poor absorption, and there is room for improvement in the anti-wrinkle effect. Therefore, there is a need to develop an anti-wrinkle skin care product that has good permeability, is easily absorbed, and can repair skin wrinkles, inhibit wrinkle regeneration, and delay skin aging. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] China Patent Application Publication No. 110755325 [Patent Document 2] China Patent Application Publication No. 104856928 Summary of the Invention [Problem to be solved by the invention]

[0007] In response to the deficiencies of the prior art, the present invention aims to provide a fully human collagen-containing anti-wrinkle composition and uses thereof. [Means for solving the problem]

[0008] In order to achieve the objectives of the present invention, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a fully human collagen-containing anti-wrinkle composition comprising fully human collagen, an amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyroylbenzylamide diacetate.

[0010] The anti-wrinkle composition of the present invention can improve the anti-wrinkle and anti-aging effects of the product by adding active ingredients such as fully human collagen, an amino acid composition, acetyl hexapeptide-8, and dipeptide diaminobutyroylbenzylamide diacetate through the synergistic effect of the above active ingredients. In particular, fully human collagen can further improve the anti-wrinkle and anti-aging effects of the combination by optimizing the nucleotide sequence of collagen, which makes it possible to prepare highly pure type I and type III collagen in E. coli cells.

[0011] Preferably, the components of the anti-wrinkle composition include, by weight, 1-10 parts of fully human collagen, 2-15 parts of an amino acid composition, 1-5 parts of acetyl hexapeptide-8, and 0.1-5 parts of dipeptide diaminobutyroylbenzylamide diacetate.

[0012] In the anti-wrinkle essence of the present invention, the amount of the complete human collagen added may be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, or 9 parts, etc. The amount of the amino acid composition added may be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, or 14 parts, etc. The amount of acetyl hexapeptide-8 added may be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc. The amount of dipeptide diaminobutyroylbenzylamide diacetate added may be 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc.

[0013] Any specific point value within each of the above numerical ranges can be selected, and the description thereof will be omitted here.

[0014] Preferably, the amino acid composition contains a combination of at least two of glycine, serine, glutamic acid, aspartic acid, leucine, alanine, lysine, arginine, tyrosine, phenylalanine, threonine, proline, valine, isoleucine, histidine, cysteine, and methionine.

[0015] Preferably, the amino acid composition is a combination of glycine, arginine, and histidine.

[0016] Preferably, the mass ratio of glycine, arginine and histidine is (1-3):(1-2):(2-3).

[0017] However, "1 to 3" may also be 1.2, 1.5, 1.8, 2, 2.2, 2.5, or 2.8, etc. "1-2" may be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc. "2 to 3" may be 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, or 2.9, etc.

[0018] Preferably, the ingredients of the anti-wrinkle composition further comprise saffron flower extract.

[0019] Preferably, the components of the anti-wrinkle composition are, by weight, 1 to 10 parts of complete human collagen, 2 to 15 parts of an amino acid composition, 1 to 5 parts of acetyl hexapeptide-8, and dipeptide diacetate. Contains 0.1 to 5 parts of dodiaminobutyroyl benzylamide and 0.1 to 5 parts of saffron flower extract.

[0020] In the anti-wrinkle composition according to the present invention, the amount of the saffron flower extract added may be 0.2 parts, 0.5 parts, 0.8 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc. Other specific point values ​​within the above numerical ranges can all be selected, and the description here is omitted.

[0021] As a preferred technical solution of the present invention, the addition of saffron flower extract to the anti-wrinkle composition not only improves the anti-wrinkle and anti-aging effects of the composition, but also plays a role in repairing and soothing the skin. When the amount added is within the limited range of the present invention, the blending effect with other ingredients is better.

[0022] In a second aspect, the present invention provides the use of a fully human collagen-containing anti-wrinkle composition as described in the first aspect in the preparation of a cosmetic product.

[0023] Preferably, the cosmetic product comprises an essence, a lotion, a milk, a cream, a cleanser or a mask.

[0024] Preferably, the mass percentage of the anti-wrinkle composition containing the complete human collagen in the cosmetic product is 1-40%, for example, 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35% or 40%, etc. Any other specific point value within each of the above numerical ranges can be selected, and the description here is omitted.

[0025] In a third aspect, the present invention provides a fully human collagen-containing anti-wrinkle essence further comprising the fully human collagen-containing anti-wrinkle composition described in the first aspect, a moisturizer, a thickener, a chelating agent, an emollient, and deionized water.

[0026] Preferably, the anti-wrinkle essence contains, by weight, 1 to 40 parts of the fully human collagen-containing anti-wrinkle composition described in the first embodiment, 5 to 20 parts of a moisturizer, 0.1 to 1 part of a thickener, 0.01 to 0.1 part of a chelating agent, 1 to 10 parts of an emollient, and deionized water.

[0027] However, the amount of the fully human collagen-containing anti-wrinkle composition added may be 1 part, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, or 40 parts, etc. The amount of the humectant added may be 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, or 19 parts, etc. The amount of the thickener added may be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, or 0.9 parts, etc. The amount of the chelating agent added may be 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, or 0.09 parts, etc. The amount of the emollient added may be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.

[0028] Any specific point value within each of the above numerical ranges can be selected, and the description thereof will be omitted here.

[0029] Preferably, the moisturizer comprises any one or a combination of at least two of glycerin, 1,3-butanediol, sodium hyaluronate, dipotassium glycyrrhizinate, glycerin polyacrylate, and sodium di(lauramidoglutamido)lysine.

[0030] Preferably, the chelating agent comprises disodium EDTA.

[0031] Preferably, the emollient comprises any one or a combination of at least two of 1,2-hexanediol, caprylyl glycol, or 1,2-pentanediol.

[0032] Preferably, the emollient is a combination of 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol.

[0033] Preferably, the mass ratio of the 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol is (0.1-0.2):(0.1-0.2):(2-4).

[0034] However, the first "0.1 to 0.2" may be 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, or 0.19, etc. The second "0.1 to 0.2" may be 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, or 0.19, etc. "2 to 4" may be 2.2, 2.5, 2.8, 3.0, 3.2, 3.5, or 3.8, etc.

[0035] Preferably, the thickening agent comprises ammonium acryloyldimethyltaurate / VP copolymer.

[0036] Preferably, the anti-wrinkle essence further comprises any one or a combination of at least two of a preservative, a pH adjusting agent, and a fragrance.

[0037] Any specific point value within each of the above numerical ranges can be selected, and the description thereof will be omitted here.

[0038] In a fourth aspect, the present invention provides a method for preparing a fully human collagen-containing anti-wrinkle essence according to the third aspect, the method comprising the steps of: (1) adding some of the humectant, thickener, chelating agent and deionized water to the aqueous phase pot, mixing and homogenizing to obtain Phase A; (2) mixing the remaining humectant and homogenizing to obtain Phase B; (3) mixing and homogenizing the emollient to obtain Phase C; and (4) adding phase A to an emulsifying pot, homogenizing it under vacuum, lowering the temperature, sequentially adding phase B, phase C, and the complete human collagen-containing anti-wrinkle composition described in the first embodiment, mixing them uniformly, and then cooling to obtain the complete human collagen-containing anti-wrinkle essence; The above steps (1), (2), and (3) do not distinguish between a chronological order.

[0039] Preferably, in step (1), the temperature during mixing is 80 to 90°C, and may be, for example, 81°C, 83°C, 85°C, 87°C, or 89°C.

[0040] Preferably, in step (1), the homogenization speed is 500 to 700 rpm, and may be, for example, 520 rpm, 550 rpm, 580 rpm, 600 rpm, 620 rpm, 650 rpm, or 680 rpm.

[0041] Preferably, in step (1), the homogenization time is 15 to 30 minutes, and may be, for example, 17 minutes, 19 minutes, 20 minutes, 22 minutes, 25 minutes, or 28 minutes.

[0042] Preferably, in step (2), the homogenization speed is 500 to 700 rpm, and may be, for example, 520 rpm, 550 rpm, 580 rpm, 600 rpm, 620 rpm, 650 rpm, or 680 rpm.

[0043] Preferably, in step (2), the homogenization time is 15 to 30 minutes, and may be, for example, 17 minutes, 19 minutes, 20 minutes, 22 minutes, 25 minutes, or 28 minutes.

[0044] Preferably, in step (3), the homogenization speed is 500 to 700 rpm, and may be, for example, 520 rpm, 550 rpm, 580 rpm, 600 rpm, 620 rpm, 650 rpm, or 680 rpm.

[0045] Preferably, in step (3), the homogenization time is 15 to 30 minutes, and may be, for example, 17 minutes, 19 minutes, 20 minutes, 22 minutes, 25 minutes, or 28 minutes.

[0046] Preferably, in step (4), the vacuum pressure is −0.05 to −0.01 MPa, and may be, for example, −0.01 MPa, −0.02 MPa, −0.03 MPa, −0.04 MPa, or −0.05 MPa.

[0047] Preferably, in step (4), the homogenization speed is 150 to 300 rpm, and may be, for example, 170 rpm, 190 rpm, 200 rpm, 220 rpm, 250 rpm, or 280 rpm.

[0048] Preferably, in step (4), the homogenization time may be 2 to 5 minutes, such as 2 minutes, 3 minutes, 4 minutes, or 5 minutes.

[0049] Preferably, in step (4), the temperature is lowered to 40 to 50°C, and may be, for example, 41°C, 43°C, 45°C, 47°C, or 49°C.

[0050] Preferably, in step (4), the mixing time is 15 to 30 minutes, and may be, for example, 17 minutes, 19 minutes, 20 minutes, 22 minutes, 25 minutes, or 28 minutes.

[0051] Preferably, in step (4), the mixture is cooled to 25-30°C, for example 25°C, 26°C, 27°C, 28°C, 29°C or 30°C.

[0052] Any specific point value within each of the above numerical ranges can be selected, and the description thereof will be omitted here.

[0053] In the present invention, the preparation method is simple, easy to operate, and has good practicality. The preparation method mixes each component in groups, and by adding a specific temperature and time, it can ensure that the active ingredients are not destroyed and can be mixed sufficiently and uniformly. If the time is too short, the mixing effect cannot be achieved, and if the time is too long, it will not only waste energy, but also cause excessive mixing of the ingredients and destroy the homogeneity of the product. Within the above limited range, the optimal effect can be achieved. Effect of the Invention

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] 1. The anti-wrinkle composition of the present invention contains active ingredients, namely, fully human collagen, amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyroylbenzylamide diacetate, and improves the anti-wrinkle effect of the product through the synergistic effect of the above active ingredients. In particular, fully human collagen can be optimized in the nucleotide sequence of collagen to prepare high purity type I and type III collagen in E. coli cells, thereby further improving the anti-wrinkle effect of the essence.

[0056] 2. The anti-wrinkle composition of the present invention, by adding saffron flower extract, can ensure anti-wrinkle and anti-aging, and also play a role in repair and soothing.

[0057] 3. The anti-wrinkle essence of the present invention can have more pronounced wrinkle-blurring, aging-retarding, repairing and soothing effects by adding the above-mentioned completely human collagen-containing anti-wrinkle composition.

[0058] 4. The method for preparing the anti-wrinkle essence of the present invention is simple and easy to operate, which facilitates industrial mass production and has excellent practicality. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0059] In order to further describe the technical means adopted in the present invention and its effects, the technical solution of the present invention will be further described below in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0060] Some material and model number information used in the following examples and comparative examples is as follows:

[0061] [Table 1]

[0062] Other ingredients may be used if purchased from a legitimate franchisor.

[0063] In the following, the weight parts of complete human collagen, amino acid composition, acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate, and saffron flower extract are calculated based on the actual content of active ingredients contained in the commercially available raw materials, which may selectively contain one or a combination of at least two of the following: solvent, filler, diluent, stabilizer, pH adjuster, antibacterial agent, antioxidant, or impurities within the permissible range.

[0064] Example 1 This embodiment provides a fully human collagen-containing anti-wrinkle composition, which contains, by weight, 5 parts fully human collagen, 10 parts amino acid composition, 2 parts acetyl hexapeptide-8, 2 parts dipeptide diaminobutyroyl benzylamide diacetate, and 2 parts saffron flower extract. However, the amino acid composition is a combination of glycine, arginine, and histidine in a mass ratio of 4:3:5.

[0065] Example 2 This embodiment provides a fully human collagen-containing anti-wrinkle composition, which contains, by weight, 1 part fully human collagen, 15 parts amino acid composition, 1 part acetyl hexapeptide-8, 5 parts dipeptide diaminobutyroyl benzylamide diacetate, and 1 part saffron flower extract. However, the amino acid composition is a combination of glycine, arginine, and histidine in a mass ratio of 3:2:3.

[0066] Example 3 This embodiment provides a fully human collagen-containing anti-wrinkle composition, which contains, by weight, 10 parts fully human collagen, 2 parts amino acid composition, 5 parts acetyl hexapeptide-8, 1 part dipeptide diaminobutyroyl benzylamide diacetate, and 5 parts saffron flower extract. However, the amino acid composition is a combination of glycine, arginine, and histidine in a mass ratio of 1:1:2.

[0067] Example 4 This embodiment provides an anti-wrinkle composition containing fully human collagen. The only difference between the ingredients of the anti-wrinkle composition and those of Example 1 is that it does not contain saffron flower extract, and the other ingredients are the same as those of Example 1.

[0068] Example 5 This embodiment provides an anti-wrinkle composition containing fully human collagen. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that the amino acid composition is a combination of glycine and arginine with a mass ratio of 4:3, and the amount of the amino acid composition added is maintained as it is, while the other components are the same as those of Example 1.

[0069] Example 6 This embodiment provides an anti-wrinkle composition containing fully human collagen. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that the amino acid composition is a combination of arginine and histidine with a mass ratio of 3:5, and the amount of the amino acid composition added is maintained as it is, while the other components are the same as those of Example 1.

[0070] Example 7 This embodiment provides an anti-wrinkle composition containing fully human collagen. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that the amino acid composition is a combination of glycine and histidine with a mass ratio of 4:5, and the amount of the amino acid composition added is maintained as it is, while the other components are the same as those of Example 1.

[0071] Example 8 This example provides an anti-wrinkle composition containing fully human collagen. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that the amino acid composition is a combination of serine, leucine, and glutamic acid in a mass ratio of 4:3:5, and the amount of the amino acid composition added is the same as that of Example 1. The other ingredients are the same as in Example 1.

[0072] Comparative Example 1 This embodiment provides an anti-wrinkle composition containing complete human collagen. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that it does not contain complete human collagen, and the amount of complete human collagen added is proportional to the amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyroyl benzylamide diacetate, while the other components are the same as those of Example 1.

[0073] Comparative Example 2 This embodiment provides a full human collagen-containing anti-wrinkle composition. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that it does not contain an amino acid composition, and the amount of the amino acid composition is proportionally distributed to full human collagen, acetyl hexapeptide-8 and dipeptide diaminobutyroyl benzylamide diacetate, while the other components are the same as those of Example 1.

[0074] Comparative Example 3 This embodiment provides a fully human collagen-containing anti-wrinkle composition. The only difference between the components of the anti-wrinkle composition and those of Example 1 is that it does not contain acetyl hexapeptide-8, and the amount of acetyl hexapeptide-8 added is proportional to the fully human collagen, amino acid composition and dipeptide diaminobutyroyl benzylamide diacetate, while the other components are the same as those of Example 1.

[0075] Comparative Example 4 This embodiment provides a fully human collagen-containing anti-wrinkle composition. The only difference between the components of the anti-wrinkle composition and those of embodiment 1 is that it does not contain dipeptide diaminobutyroyl benzylamide diacetate, and the amount of dipeptide diaminobutyroyl benzylamide diacetate is proportional to fully human collagen, acetyl hexapeptide-8 and amino acid composition, while the other components are the same as those of embodiment 1.

[0076] Application example 1 This application example provides a completely human collagen-containing anti-wrinkle essence, the ingredients of which, by weight, include 5 parts of glycerin, 4 parts of 1,3-butanediol, 0.1 parts of sodium hyaluronate, 0.1 parts of dipotassium glycyrrhizinate, 0.03 parts of disodium EDTA, 1 part of glycerin polyacrylate, 0.4 parts of ammonium acryloyldimethyltaurate / VP copolymer, 0.1 parts of sodium di(lauramidoglutamide)lysine, 0.20 parts of 1,2-hexanediol, 0.15 parts of caprylyl glycol, 4 parts of 1,2-pentanediol, 21 parts of the completely human collagen-containing anti-wrinkle composition provided in Example 1, and 63.92 parts of deionized water.

[0077] The preparation method of the fully human collagen-containing anti-wrinkle essence includes the following steps:

[0078] (1) 1,3-butanediol, sodium hyaluronate, dipotassium glycyrrhizinate, disodium EDTA, glycerin polyacrylate, ammonium acryloyldimethyltaurate / VP copolymer and deionized water were added to an aqueous phase pot, mixed, heated to 85°C, and homogenized at 600 rpm for 20 minutes to obtain phase A.

[0079] (2) Glycerin and sodium di(lauramidoglutamido)lysine were mixed at 600 rpm and homogenized for 25 minutes to obtain Phase B.

[0080] (3) 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol were mixed at 600 rpm and homogenized for 15 minutes to obtain Phase C.

[0081] (4) Phase A was placed in an emulsifying pot and homogenized at 200 rpm for 3 minutes under a vacuum pressure of -0.03 MPa. The temperature was then lowered to 45°C. Phase B, Phase C, and the fully human collagen-containing anti-wrinkle composition of Example 1 were then sequentially added and stirred for 20 minutes. After the mixture became homogeneous, it was cooled to 25°C to obtain the fully human collagen-containing anti-wrinkle essence.

[0082] Application example 2 This application example provides a fully human collagen-containing anti-wrinkle essence. The only difference between the anti-wrinkle essence and application example 1 is that the fully human collagen-containing anti-wrinkle composition is the fully human collagen-containing anti-wrinkle composition provided in Example 2.

[0083] The preparation method of the fully human collagen-containing anti-wrinkle essence includes the following steps:

[0084] (1) 1,3-butanediol, sodium hyaluronate, dipotassium glycyrrhizinate, disodium EDTA, glycerin polyacrylate, ammonium acryloyldimethyltaurate / VP copolymer and deionized water were added to an aqueous phase pot, mixed, heated to 85°C, and homogenized at 600 rpm for 20 minutes to obtain phase A.

[0085] (2) Glycerin and sodium di(lauramidoglutamido)lysine were mixed at 600 rpm and homogenized for 25 minutes to obtain Phase B.

[0086] (3) 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol were mixed at 600 rpm and homogenized for 15 minutes to obtain Phase C.

[0087] (4) Phase A was placed in an emulsifying pot and homogenized at -0.03 MPa vacuum pressure and 200 rpm for 3 minutes. The temperature was then lowered to 45°C. Phase B, Phase C, and the fully human collagen-containing anti-wrinkle composition of Example 2 were then sequentially added and mixed with stirring for 20 minutes. After the mixture became homogeneous, it was cooled to 25°C to obtain the fully human collagen-containing anti-wrinkle essence.

[0088] Application example 3 This application example provides a fully human collagen-containing anti-wrinkle essence. The only difference between the anti-wrinkle essence and application example 1 is that the fully human collagen-containing anti-wrinkle composition is the fully human collagen-containing anti-wrinkle composition provided in Example 3.

[0089] The preparation method of the fully human collagen-containing anti-wrinkle essence includes the following steps:

[0090] (1) 1,3-butanediol, sodium hyaluronate, dipotassium glycyrrhizinate, disodium EDTA, glycerin polyacrylate, ammonium acryloyldimethyltaurate / VP copolymer and deionized water were added to an aqueous phase pot, mixed, heated to 85°C, and homogenized at 600 rpm for 20 minutes to obtain phase A.

[0091] (2) Glycerin and sodium di(lauramidoglutamido)lysine were mixed at 600 rpm and homogenized for 25 minutes to obtain Phase B.

[0092] (3) 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol were mixed at 600 rpm and homogenized for 15 minutes to obtain Phase C.

[0093] (4) Phase A was placed in an emulsifying pot and homogenized at -0.03 MPa vacuum pressure and 200 rpm for 3 minutes. The temperature was then lowered to 45°C. Phase B, Phase C, and the fully human collagen-containing anti-wrinkle composition of Example 3 were then sequentially added and stirred for 20 minutes. After the mixture became homogeneous, it was cooled to 25°C to obtain the fully human collagen-containing anti-wrinkle essence.

[0094] Application examples 4 to 8 These application examples provide five kinds of anti-wrinkle essences, and the only difference from application example 1 is that the fully human collagen-containing anti-wrinkle composition in example 1 is replaced with the fully human collagen-containing anti-wrinkle composition provided in examples 4 to 8 in an equal amount, while the other components and contents are maintained as they are. The preparation method is the same as application example 1.

[0095] Application example 9 This application example provides a fully human collagen-containing anti-wrinkle essence. The difference between the anti-wrinkle essence and application example 1 is only that the emollient is a composition of 1,2-hexanediol and caprylyl glycol, and the amount of the emollient added is maintained as it is, the mass ratio of 1,2-hexanediol and caprylyl glycol is the same as application example 1, and other ingredients and contents are maintained as they are. The preparation method is the same as application example 1.

[0096] Application example 10 This application example provides an anti-wrinkle essence containing completely human collagen. The only difference between the anti-wrinkle essence and application example 1 is that the emollient is a composition of 1,2-hexanediol and 1,2-pentanediol, and the amount of the emollient added is maintained as is, the mass ratio of 1,2-hexanediol to 1,2-pentanediol is the same as in application example 1, and other components and contents are maintained as is. The preparation method is the same as application example 1.

[0097] Application example 11 This application example provides an anti-wrinkle essence containing fully human collagen. The difference between the anti-wrinkle essence and application example 1 is only that the emollient is a composition of 1,2-pentanediol and caprylyl glycol, and the amount of the emollient added is maintained as it is, the mass ratio of 1,2-pentanediol and caprylyl glycol is the same as application example 1, and other components and contents are maintained as they are. The preparation method is the same as application example 1.

[0098] Comparative application examples 1-4 These comparative application examples provide four kinds of anti-wrinkle essences. The difference from application example 1 is only that the complete human collagen-containing anti-wrinkle composition in example 1 is replaced with the complete human collagen-containing anti-wrinkle composition provided in comparative examples 1 to 4 in the same amount, while the other components and contents are maintained as they are. The preparation method is the same as application example 1.

[0099] Comparative application example 5 This comparative application example provides an anti-wrinkle essence. The difference from application example 1 is that the ingredients do not contain the anti-wrinkle composition containing complete human collagen, and the amount of the anti-wrinkle composition that is lost is fully supplemented with deionized water, while the other ingredients and contents are maintained as they are. The preparation method is the same as application example 1.

[0100] [Test Example 1] Antioxidant effect research - DPPH radical scavenging test Accurately weigh out 47.7 mg of DPPH reagent and dilute it into a 100 mL brown measuring flask. Take 5 mL of it and dilute it into a 50 mL brown measuring flask with absolute ethanol to obtain the DPPH test solution (47.7 mg / L). Take out the H test solution, add 0.1 mL of each of the wrinkle-preventing compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 4 as shown in Table 1, and then add an appropriate amount of anhydrous ethanol so that the total mass is equal. After thorough mixing, leave it in the dark for 30 minutes. Using anhydrous ethanol as a blank, the absorbance was measured at 517 nm, and using vitamin C as a control, the DPPH scavenging rate of each extract was calculated using the formula DPPH scavenging rate (%) = [1-(As-Ar) / A0] x 100%.

[0101] Where: As - absorbance of the reaction system of extract and DPPH; Ar - absorbance of extract-absolute ethanol; A0 - absorbance of absolute ethanol as blank.

[0102] [Table 2]

[0103] The DPPH radical scavenging rate reflects the antioxidant ability to a certain extent, and the higher the scavenging rate, the better the antioxidant level and the better the anti-aging effect. Therefore, the anti-aging effect of an essence can be judged by studying its DPPH radical scavenging ability. Vitamin C is a highly efficient antioxidant recognized in the cosmetics industry, and in this test, vitamin C was used as a control to consider its antioxidant ability. The results are shown in Table 2.

[0104] [Table 3]

[0105] As can be seen from Table 2, Examples 1 to 8 and Comparative Examples 1 to 4 all have good scavenging effects against DPPH, and all of the scavenging rates are 60% or more. Among them, Example 1 has a higher scavenging rate than Comparative Examples 1 to 4, indicating that the composition has better antioxidant ability. However, the complete human collagen, amino acid composition, acetyl hexapeptide-8, and dipeptide diaminobutyroyl benzylamide diacetate promote each other in antioxidant effect, and have an unexpected synergistic effect, verifying the rationality of the ingredient combination. And, the scavenging rates of Examples 1 to 3 are slightly lower and very close to those of the positive control vitamin C, which indicates that the anti-wrinkle composition of the present invention has good radical scavenging and antioxidant effects, and the composition has antioxidant ability close to that of vitamin C, and has good antioxidant effect.

[0106] [Test Example 2] Skin improvement and tightening test The anti-wrinkle essence combinations prepared in Application Examples 1 to 11 and Comparative Application Examples 1 to 5 were evaluated for their skin tightening effects.

[0107] 160 subjects aged 20-45 were randomly selected and divided into 16 groups with 10 subjects in each group. The subjects in the 16 groups used the anti-wrinkle essences prepared in Application Examples 1-11 and Comparative Application Examples 1-5 (once in the morning and once in the evening) for 30 days. The subjects were not allowed to use other cosmetics that maintain skin elasticity or affect the results during the test period, and did not change their usual eating and drinking habits during the test period.

[0108] Using a skin elasticity measuring device Cutometer MPA580, the skin elasticity was measured on the 0th and 3rd days, respectively. The skin condition on day 0 was measured. The test site was the cheek. During the measurement, the probe was measured three times on the same test area, and the average value was taken. The test parameter was R2 (the ratio of the skin elasticity Ua when there is no negative pressure to the maximum elongation Uf when there is negative pressure), and the closer R2 is to 1, the better the skin elasticity is, and the average R2 change value = R2 value on day 30 - R2 value on day 0, and the average R2 change rate = R2 average change value / R2 value on day 0. Specific skin elasticity test results are shown in Table 3.

[0109] [Table 4]

[0110] As a result, the anti-wrinkle essence of the present invention has a good skin tightening effect, and the complete human collagen, amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyroylbenzylamide diacetate in it mutually promote the antioxidant effect, have an unexpected synergistic effect, and the type of emollient also affects the skin tightening effect of the essence.

[0111] [Test Example 3] Testing to improve skin wrinkles The specific test method is as follows.

[0112] 160 women aged 30-40 with aging skin were selected and randomly divided into 16 groups, 10 people per group, and anti-wrinkle essences in Application Examples 1-11 and Comparative Application Examples 1-5 were used. The subjects were required to use the product continuously, applying it twice a day to the face in the morning and evening, for 90 consecutive days, and were required to revisit the study on the 30th (D30) and 90th (D90) days. For the same subjects, the number and length of facial wrinkles were counted by the same measuring staff, and the improvement of skin wrinkles was calculated based on the evaluation standard.

[0113] Evaluation standard: 10 points: No wrinkles. 7-9 points: 2-3 wrinkles, less than 1.5 cm long. 4-6 points: 3-6 wrinkles, less than 3cm long. 1-3 points: Multiple main wrinkles, greater than 4 cm in length, accompanied by shallow wrinkles.

[0114] The test results are shown in Table 4.

[0115] [Table 5]

[0116] [Table 6]

[0117] As a result, as can be seen from Application Examples 1 to 3, the anti-wrinkle essence of the present invention has a good wrinkle removal effect. As can be seen from Application Example 4, after adding saffron flower extract, the wrinkle removal effect of the essence can be further improved. As can be seen from Comparative Application Examples 1 to 4, the complete human collagen, amino acid composition, acetyl hexapeptide-8 and dipeptide diaminobutyroyl benzylamide diacetate of the present invention have a wrinkle removal effect. As can be seen from Comparative Application Example 5, when the essence does not contain the full human collagen-containing anti-wrinkle composition, its wrinkle removal effect is obviously poor.

[0118] [Test Example 4] Safety evaluation test

[0119] Safety assessment The specific method is as follows. 40 women aged 18-35 with no history of allergies were selected. Each subject tried the anti-wrinkle compositions prepared in all of Examples 1-8. After washing the subject's back, the patch test device containing the sample was attached to the designated position on the back with non-irritating tape. After attachment, the patch test device was lightly pressed with a finger to evenly attach it to the skin and maintained for 48 hours. The subject must keep the patch site dry within 48 hours and avoid strenuous exercise, scratching the patch test site, and prolonged exposure to sunlight. After 48 hours, the test device was removed and a mark was made. After 30 minutes, the indentation disappeared, and the evaluation was made under sufficient light.

[0120] The skin adverse reaction level standards are shown in Table 5.

[0121] [Table 7]

[0122] The results are shown in Table 6.

[0123] [Table 8]

[0124] As can be seen from the data in Table 6, all subjects had negative reactions, and it was determined that the fully human collagen-containing anti-wrinkle essence of the present invention did not irritate the skin and did not cause any positive reactions, indicating that the product of the present invention is safer and milder.

[0125] The present invention has described the anti-wrinkle composition containing complete human collagen and its use through the above examples, but the present invention is not limited to the above examples, that is, the present invention does not have to be carried out according to the above examples, the applicant declares. It is clear to those skilled in the art that any improvements to the present invention, equivalent replacement of each raw material of the product of the present invention, addition of auxiliary ingredients, selection of specific embodiments, etc., are all within the protection scope and disclosure scope of the present invention.

[0126] Although the preferred embodiments of the present invention have been described in detail above, the present invention is not limited to the specific details of the above embodiments. Within the technical concept of the present invention, a number of simple modifications can be made to the technical solution of the present invention, and all of these simple modifications belong to the protection scope of the present invention.

[0127] It should be noted that the specific technical features described in the above specific embodiments can be combined in any suitable manner unless they are contradictory, and in order to avoid unnecessary repetition, the present invention does not separately describe various possible combinations.

Claims

1. A fully human collagen-containing anti-wrinkle composition comprising: The ingredients of the anti-wrinkle composition include, by weight, 1-10 parts fully human collagen, 2-15 parts amino acid composition, 1-5 parts acetyl hexapeptide-8, and 0.1-5 parts dipeptide diaminobutyroyl benzylamide diacetate; The amino acid composition is a combination of glycine, arginine, and histidine in a mass ratio of (1-3):(1-2):(2-3). A fully human collagen-containing anti-wrinkle composition.

2. The ingredients of the anti-wrinkle composition further include saffron flower extract.

2. The fully human collagen-containing anti-wrinkle composition according to claim 1.

3. The ingredients of the anti-wrinkle composition include, by weight, 1-10 parts of fully human collagen, 2-15 parts of an amino acid composition, 1-5 parts of acetyl hexapeptide-8, 0.1-5 parts of dipeptide diaminobutyroyl benzylamide diacetate, and 0.1-5 parts of saffron flower extract.

3. The fully human collagen-containing anti-wrinkle composition according to claim 2.

4. 4. Use of the fully human collagen-containing anti-wrinkle composition according to any one of claims 1 to 3 in the manufacture of cosmetics.

5. The cosmetic product includes an essence, a lotion, a milky lotion, a cream, a facial cleanser or a mask.

5. The use according to claim 4.

6. The anti-wrinkle composition containing completely human collagen has a mass percentage of 1 to 40% in the cosmetic product.

5. The use according to claim 4.

7. A wrinkle-preventing composition containing a fully human collagen according to any one of claims 1 to 3, comprising a moisturizing agent, a thickening agent, a chelating agent, an emollient, and deionized water. A fully human collagen-containing anti-wrinkle essence.

8. The anti-wrinkle essence contains, by weight, 1 to 40 parts of the fully human collagen-containing anti-wrinkle composition according to any one of claims 1 to 3, 5 to 20 parts of a moisturizing agent, 0.1 to 1 part of a thickening agent, 0.01 to 0.1 part of a chelating agent, 1 to 10 parts of an emollient, and deionized water.

8. The anti-wrinkle essence containing fully human collagen according to claim 7.

9. The moisturizing agent includes any one or a combination of at least two of glycerin, 1,3-butanediol, sodium hyaluronate, dipotassium glycyrrhizinate, glycerin polyacrylate, and sodium di(lauramidoglutamido)lysine.

8. The anti-wrinkle essence containing fully human collagen according to claim 7.

10. The chelating agent includes disodium EDTA.

8. The anti-wrinkle essence containing fully human collagen according to claim 7.

11. The emollient comprises any one or a combination of at least two of 1,2-hexanediol, caprylyl glycol, or 1,2-pentanediol.

8. The anti-wrinkle essence containing fully human collagen according to claim 7.

12. The emollient is a combination of 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol.

12. The anti-wrinkle essence containing fully human collagen according to claim 11.

13. the mass ratio of the 1,2-hexanediol, caprylyl glycol and 1,2-pentanediol is (0.1-0.2):(0.1-0.2):(2-4); 13. The fully human collagen-containing anti-wrinkle essence according to claim 12.

14. The thickener comprises ammonium acryloyldimethyltaurate / VP copolymer; 8. The anti-wrinkle essence containing fully human collagen according to claim 7.

15. The anti-wrinkle essence further contains any one or a combination of at least two of a preservative, a pH adjuster, and a fragrance.

8. The anti-wrinkle essence containing fully human collagen according to claim 7.

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