Oral Compositions

Abstract

To provide a barley young leaf-containing oral composition that has useful functions.SOLUTION: An oral composition contains barley young leaves, and at least two materials selected from protein, gymnema, acesulfame potassium and sucralose.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an oral composition containing young barley leaves. [Background technology]

[0002] 2. Description of the Related Art In recent years, with an increasing interest in health and other factors, various health foods have been developed, and research is being conducted into various materials to be used in health foods.

[0003] For example, young barley leaves are commonly used as an ingredient in health foods and green juice, and have been widely studied for their functionality, such as laxative effects and antihypercholesterolemic effects (Patent Documents 1 to 3).

[0004] In addition, in today's world where people are highly safety-conscious as well as health-conscious, products made from safe ingredients such as young barley leaves, which are eaten on a daily basis, are preferred, and there is a desire to make better use of the useful functions of such safe ingredients, such as young barley leaves, which are commonly eaten. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent Publication 2006-151951 [Patent Document 2] Patent Publication No. 2001-314170 [Patent Document 3] Patent No. 4183886 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide an oral composition containing young barley leaves that has useful functions. [Means for solving the problem]

[0007] The inventors discovered that the addition of two specific ingredients (other ingredients) to young barley leaves results in excellent myoblast proliferation and excellent α-glucosidase inhibitory effects, and thus completed the present invention.

[0008] That is, the present invention is as follows. [1] An oral composition comprising young barley leaves and at least two other ingredients selected from protein, gymnema, acesulfame potassium and sucralose. [2] The oral composition according to [1] above, characterized in that it is used for dieting. Effect of the Invention

[0009] The oral composition of the present invention has an excellent myoblast proliferation effect and / or an excellent α-glucosidase inhibitory effect, and is therefore effective for maintaining and strengthening muscle tissue, suppressing an increase in blood sugar level, and dieting. [Brief description of the drawings]

[0010] [Figure 1] 1 is a graph showing the results of myoblast proliferation rate when the composition of the present invention (young barley leaves + two other materials) was applied. [Diagram 2] 1 is a graph showing the results of α-glucosidase inhibition rate when the composition of the present invention (young barley leaves + two other materials) was applied. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] The oral composition of the present invention is characterized by containing young barley leaves and at least two other ingredients selected from protein, gymnema, acesulfame potassium and sucralose.

[0012] The oral composition of the present invention has an excellent myoblast proliferation effect and can maintain and strengthen muscle tissue. In addition, since muscle tissue is a tissue with active energy metabolism, the maintenance and strengthening of muscle tissue can maintain and increase basal metabolic rate, which can provide a diet effect by preventing and / or eliminating obesity.

[0013] Similarly, the oral composition of the present invention has an excellent α-glucosidase inhibitory effect and can delay the decomposition and absorption of sugar in the body (intestinal tract), thereby suppressing the rise in blood glucose level after a meal. In addition, by delaying the decomposition and absorption of sugar in the body (intestinal tract), a diet effect can be obtained.

[0014] In addition, the oral composition of the present invention containing young barley leaves and at least two other materials has higher dispersibility than the case where young barley leaves are used or the case where young barley leaves and one other material are used. That is, the oral composition of the present invention is a powder (powder) that does not easily aggregate. Therefore, when dissolved in liquid such as water or hot water and ingested, it is easy to dissolve and can be easily mixed. Furthermore, in the manufacturing process of the product, adhesion between powders is suppressed in the powder mixing process, and the formation of lumps is suppressed, making it possible to mix uniformly. That is, the oral composition of the present invention with high dispersibility can be prepared simply by mixing, and is easy to prepare. For example, when mixing raw materials that are highly cohesive and prone to forming lumps, it is known that the raw materials are pretreated, such as crushed using a grinder, to ensure the flowability of the raw materials before being charged, and further, after charging, it is necessary to supply air as quickly as possible to prevent re-agglomeration and start flow, but such a complicated operation is not necessary. It is also known that adhesion can be reduced by premixing raw materials that are highly cohesive and prone to forming lumps with an excipient, but such addition is not necessary.

[0015] Furthermore, since the composition of the present invention has high dispersibility, even when materials other than the components of the present invention are mixed to prepare the oral composition of the present invention, there is no need to carry out the complicated operations described above, and the composition can be prepared simply by mixing.

[0016] [Young barley leaves] Barley (Hordeum vulgare L.) used as a raw material in the present invention is said to be native to Central Asia, is an annual or biennial herb belonging to the family Poaceae, and is roughly classified into two-row barley, six-row barley, etc., depending on the ear shape. The barley used in the present invention is not particularly limited as long as it is normally available, and any barley such as two-row barley or six-row barley may be used. In addition, any variety may be used. The young barley leaves may include young barley leaves, and may also include other parts such as stems.

[0017] Barley young leaves can be used as pulverized material, juice, extract, etc. Examples of pulverized material include dried powder, shredded material, and dried products thereof (dried shredded material). The juice or extract may be in liquid form, but can also be used in paste form or dried powder form (squeezed juice powder, extract powder). The extract can be obtained by extraction using a suitable solvent, and examples of the solvent that can be used include water (warm water, hot water), ethanol, and aqueous ethanol. In the present invention, the nutritional components contained in young barley leaves can be effectively utilized, and thus the barley leaves are preferably dried and pulverized into a powder, with the dry powder preferably having a particle size of 75 μm or less at 60%, more preferably 75% or more, and particularly preferably 90% or more.

[0018] [Other materials] (protein) The protein is extracted and purified from proteins contained in animals and plants, and commercially available products can be used. In the present invention, powdered products are preferred. Examples of animal-derived proteins include whey protein, casein protein, egg protein, and beef protein, and examples of plant-derived proteins include soy protein, pea protein, and rice protein. In the present invention, it is preferred to use plant-derived proteins, and it is particularly preferred to use soybean-derived proteins (soy protein) because they exert a particularly excellent myoblast proliferation effect when combined with young barley leaves. In addition, it is preferred to use soy protein with a protein content of 50% or more, preferably 70% or more, and particularly preferably 90% or more, on a dry matter basis, because they exert a particularly excellent dispersibility improvement effect when combined with young barley leaves.

[0019] (Gymnema) Gymnema is a vine plant belonging to the Apocynaceae family native to India and Sri Lanka, and an example thereof is Gymnema sylvestre. The part used in the present invention is preferably the leaves. In addition, the leaves may also include stems. Gymnema can be used as a pulverized product, a squeezed juice, an extract, or the like. Examples of the pulverized product include a dried powder, a finely shredded product, and a dried product thereof (dried finely shredded product). The squeezed juice or extract may be in a liquid form, but can also be used as a paste or a dried powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using a suitable solvent, and examples of the solvent that can be used include water (hot water), ethanol, and aqueous ethanol. In the present invention, a dried powder of gymnema is preferred, and it is more preferred to use a dried powder of an aqueous alcohol extract of gymnema leaves, since it exerts a particularly excellent myoblast proliferation effect when combined with young barley leaves. Furthermore, since the dry powder of Gymnema leaves exhibits particularly excellent dispersibility improving effect when combined with young barley leaves, the gymnemic acid content is preferably 5 to 80%, more preferably 10 to 60%, and particularly preferably 20 to 40%.

[0020] (acesulfame potassium) Acesulfame potassium (acesulfame K) is an artificial sweetener, and is a high-intensity sweetener with a sweetness 200 times that of sucrose. Chemically, it is the potassium salt of 6-methyl-1,2,3-oxathiazin-4(3H)-one-2,2-dioxide. In the present invention, various forms such as liquid, powder, solid, granule, etc. can be used, and commercially available products can be used. In the present invention, a powder form is preferred, and since it exerts a particularly excellent dispersibility improving effect when combined with young barley leaves, it is preferable that the particle size is 100 μm or less in 80% or more, more preferably 90% or more, and particularly preferably 99% or more.

[0021] (Sucralose) Sucralose is a safe, non-caloric, high-intensity sweetener produced from sucrose and having a sweetness approximately 600 times that of sucrose. In the present invention, various forms such as liquid, powder, solid, and granules can be used, and commercially available products can be used. In the present invention, powder is preferred, and since it exerts a particularly excellent effect of improving dispersibility when combined with young barley leaves, it is preferred that 80% or more of the particles have a particle size of 20 mesh or less, more preferably 90% or more, and particularly preferably 100%.

[0022] The oral composition of the present invention can be used as a general food, food additive, feed, etc., and can also be used as a functional food, such as a specified health food, a nutrient functional food, or a food with functional claims, whose efficacy has been approved by a designated organization, such as a food for specified health uses, a food with nutritional function claims, or a food with functional claims, since it has an effect of maintaining and enhancing muscle tissue based on an excellent myoblast proliferation effect and an α-glucosidase inhibitory effect, and a diet (anti-obesity) effect. It can also be used as a medicine (including quasi-drugs).

[0023] That is, the oral composition of the present invention can be used as an oral diet composition for dieting, for example, so-called health foods and medicines, and such oral diet compositions are not particularly limited as long as they contain young barley leaves and two other materials and can be distinguished from other products in terms of being used for dieting. For example, the scope of the present invention includes products that display the diet function on the main body, packaging, instructions, or advertising materials of the product of the present invention. Note that the oral diet composition of the present invention is not limited to products that display the components of the present invention (young barley leaves and two other materials) as active ingredients for dieting on the product packaging, etc. For example, the active ingredient may not be specified, or a specific ingredient may be displayed as the active ingredient.

[0024] Specifically, in the case of so-called health foods, examples include products labeled with claims such as "for those concerned about their weight," "boosts metabolism," "helps obese people reduce body fat," "boosts the ability to metabolize fat," and "supports slimming."

[0025] The oral composition of the present invention can likewise be used as an oral composition for maintaining and enhancing muscle tissue, and an oral composition for suppressing an increase in blood sugar level.

[0026] Examples of the form of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, chewables, and sticks. Among these, tablets, capsules, powders, granules, and liquids are particularly preferred. Specific examples include supplements, containerized beverages filled in PET bottles, cans, bottles, etc., instant beverages (powdered beverages) to be dissolved in water (hot water), milk, fruit juice, green juice, etc., and food additives. These are preferred because they are easy to drink during meals and can increase palatability.

[0027] The contents of young barley leaves and two other materials (ingredients of the present invention) in the oral composition of the present invention may be appropriately determined within the range in which the desired effect is achieved.

[0028] In general, when the oral composition of the present invention is a supplement or pharmaceutical product in the form of a tablet or other pill, the components of the present invention are preferably contained in an amount of 0.01 to 100 mass%, more preferably 0.1 to 80 mass%, and even more preferably 1 to 60 mass%, of the total composition calculated as dry mass.

[0029] When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.001 to 30 mass%, more preferably 0.01 to 20 mass%, and even more preferably 0.1 to 15 mass%, of the total composition.

[0030] Furthermore, when the oral composition of the present invention is an instant drink such as green juice, the component of the present invention is contained in an amount of preferably 0.01 to 100 mass%, more preferably 0.1 to 80 mass%, and even more preferably 1 to 60 mass%, of the entire composition calculated on a dry mass basis.

[0031] In order to more effectively exert the effects of the present invention, the components of the present invention are preferably contained in an amount of 70% or more, more preferably 80% or more, even more preferably 90% or more, and particularly preferably 100% of the total oral composition of the present invention, calculated on a dry mass basis.

[0032] The intake amount of the oral composition of the present invention is not particularly limited, but from the viewpoint of more significantly exerting the effects of the present invention, it is preferable that the intake amount of barley leaves per day for an adult is 0.01 g / day or more in dry mass equivalent, more preferably 0.1 g / day or more, and even more preferably 1 g / day or more. The upper limit is, for example, 30 g / day, preferably 20 g / day, and more preferably 10 g / day. The oral composition of the present invention can be stored as a daily amount in one container or divided into, for example, 2 to 3 multiple containers so that the daily intake amount is the above-mentioned intake amount.

[0033] The blending mass ratio of the young barley leaves and the two other ingredients (total amount) is preferably 0.0001:1 to 10000:1, more preferably 0.001:1 to 1000:1, and even more preferably 0.01:1 to 100:1, calculated on a dry mass basis.

[0034] The oral composition of the present invention can be prepared by a known method by adding other ingredients than the ingredients of the present invention as necessary. Examples of other ingredients than the ingredients of the present invention include vitamins such as water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P) and oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus, and iron; sulfur-containing compounds contained in taurine and garlic; flavanoids or flavonoids such as hesperidin and quercetin; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; crushed products or extracts of animals and plants, etc.

[0035] The oral composition of the present invention exhibits the myoblast proliferation promoting effect shown in the examples described later, and therefore can be an excellent composition for myoblast proliferation, muscle building, muscle mass increase, energy metabolism activation, diet composition, adipose tissue reduction, and body fat reduction. Also, the oral composition of the present invention exhibits the α-glucosidase inhibitory effect shown in the examples described later, and therefore can be an excellent composition for α-glucosidase inhibition, blood glucose level rise suppression, and diet composition. Furthermore, the oral composition of the present invention exhibits the dispersibility improving effect shown in the examples described later, and therefore can be a composition that is less likely to aggregate and has high dispersibility. EXAMPLES

[0036] The present invention will be described below with reference to examples. [Example 1] (raw materials) As the young barley leaves, dried powder of young barley leaves (90% or more having a particle size of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.) prepared by drying and powdering young barley leaves was used. The protein used was powdered isolated soy protein (protein content 90% or more on a dry matter basis; commercially available product) obtained by neutralizing the curd obtained by acid precipitation of soy milk extracted from defatted soybeans, drying it, and sieving it through a 20-mesh sieve. Gymnema sylvestre powder (90% or more on 40 mesh or less, 85% or more on 80 mesh or less, gymnemic acid content 25-30%; commercially available product) was used, which was made by extracting Gymnema sylvestre leaves with aqueous ethanol, drying the extract, and powdering it. The acesulfame potassium used was acesulfame potassium powder (particle size 100 μm; commercially available product) obtained by a synthetic reaction between diketene, a substance derived from acetic acid, and sulfamic acid, followed by cyclization with sulfuric acid and neutralization with potassium hydroxide, followed by purification, concentration, and crystallization. The sucralose used was sucralose powder (commercially available product) obtained by passing powdered sucralose through a 20 mesh sieve.

[0037] [Test Example 1] (Mouse myoblast proliferation assay) In a 37℃, 5% CO2 incubator, 75 cm 2 Mouse myoblast cells (C2C12) were cultured in 10% FBS-DMEM using a flask. The cells were suspended by trypsinization and then placed in a 75 cm 2 The cells were seeded from the flask into each well of a collagen-coated 96-well plate at a cell density of 8,000 cells per well.

[0038] After pre-incubation for 24 hours in a 5% CO2 incubator at 37°C, 100 µL of the test substance prepared in 10% FBS-DMEM was added to the wells. Specifically, the test substances were prepared and added as follows. First, the test substance was suspended in 10% FBS-DMEM at 5 mg / ml and filtered through a 0.2 μm filter. Next, this 5 mg / ml suspension of the test substance was further diluted with 10% FBS-DMEM and added to the wells to the concentrations (μg / mL) shown in Table 1.

[0039] [Table 1]

[0040] After 24 hours of incubation, the cells were washed twice with PBS, and 100 μL of filter-sterilized MTT solution (MTT 500 μg / ml in DMEM) was added, followed by further incubation for 3 hours. The cells were washed twice with PBS, and 100 μL of 0.04 N HCl in isopropanol was added.

[0041] After confirming that the dye had been released outside the cells, the absorbance (570 nm, 630 nm) was measured to determine the cell proliferation rate (% of control).

[0042] % of control =[SampleDate] / [ControlDate]×100

[0043] Data =[(Sample570nm-Sample630nm)-(blank570nm-blank630nm)]

[0044] Sample 570nm and blank 570nm are absorbance values ​​at 570nm, and sample 630nm and blank 630nm are absorbance values ​​at 630nm (turbidity).

[0045] The results are shown in Figure 1.

[0046] As shown in Fig. 1, the compositions of the present invention (Examples 1 to 4) in which young barley leaves were blended with two other ingredients showed a significant improvement in cell proliferation rate. In particular, when young barley leaves were combined with protein and sucralose (Example 3) and when young barley leaves were combined with Gymnema and sucralose (Example 4), the increase was significant compared to Examples 1 and 2. When one other ingredient was used (Comparative Examples 6 to 9), no significant improvement in cell proliferation rate was observed compared to the comparative examples.

[0047] [Test Example 2] (α-glucosidase inhibition test) Each test substance was weighed into a 1.5 ml tube, 0.1 M PB was added to the tube so as to obtain the specified final concentration shown in Table 2, and the tube was sonicated for 10 minutes. After centrifugation (10,000 rpm, room temperature, 5 minutes), the supernatant was collected and used as the sample solution.

[0048] [Table 2]

[0049] A sample solution (0.1 M PB as a control) was added to a 96-well plate at 80 μL / well. A substrate solution was added at 40 μL, and the plate was incubated at 37° C. for 5 minutes.

[0050] 40 μL of the enzyme solution was added to the test wells and pre-incubated at 37° C. for 15 minutes, after which 160 μL of the reaction stop solution was added. For the blank, the reaction stop solution was added, followed by the addition of 40 μL of the enzyme solution.

[0051] The absorbance at 440 nm was measured using a microplate reader. The inhibition rate was calculated using the measured absorbance according to the following formula.

[0052] Inhibition rate (%) =(1-[(Sample test -Sample blank ) / (Control test -Control blank )]) × 100

[0053] The substrate solution used was 0.02M p-nitrophenyl α-D-glucopyranoside in water, the enzyme solution used was 0.02U / ml α-glucosidase in 0.01M PB (pH 7.0) containing 0.2% BSA, and the reaction stop solution used was 0.2M sodium carbonate in water.

[0054] The results are shown in Figure 2.

[0055] As shown in FIG. 2, the composition of the present invention, which contains young barley leaves and two other ingredients, showed a synergistic improvement in the α-glucosidase inhibition rate.

[0056] [Test Example 3] (Dispersibility test) Each raw material was sieved (nominal size: 710). When combining two or more raw materials, the amount required for measuring the degree of dispersion was measured according to the ratios in Tables 6 and 7, and the mixture was mixed in a plastic bag by shaking by hand until it was uniform.

[0057] 10 g of the test substance was weighed out and the degree of dispersion was measured using a powder specific evaluation device "Powder Tester PT-X" manufactured by Hosokawa Micron Corp. The measurement of the degree of dispersion was carried out according to the manual of the device.

[0058] The degree of dispersion is measured by the following method. A sample of a specified weight (W) is dropped onto a tray from a specified height, and the weight (W1) of the sample on the tray is measured. Furthermore, the degree of dispersion is calculated by the following formula.

[0059] Dispersion degree (%)=(W-W1) / W×100

[0060] The composition of each test substance and the results are shown in Tables 7 to 12.

[0061] [Table 3]

[0062] [Table 4]

[0063] [Table 5]

[0064] [Table 6]

[0065] [Table 7]

[0066] [Table 8]

[0067] As shown in Tables 3 to 8, the compositions of the present invention in which young barley leaves were blended with two other materials all had a higher degree of dispersion and improved dispersibility than young barley leaves alone.

[0068] [Combination example] The powdered composition for consumption (oral composition of the present invention) shown in Table 9 was prepared. When 3 g of the obtained composition of the present invention was mixed with 150 mL of water and consumed, the odor and taste of young barley leaves were improved, and a beverage with excellent overall palatability was obtained.

[0069] [Table 9] [Industrial Applicability]

[0070] The oral composition of the present invention can be used as a so-called health food or the like, and is therefore industrially useful.

Claims

[Claim 1] An oral composition comprising barley leaves, a protein, and a high-intensity sweetener, the high-intensity sweetener being at least one selected from acesulfame potassium and sucralose, Aojiru contains powdered soy protein, water-soluble dietary fiber, barley leaf powder, salt, spirulina powder, copper-containing yeast, zinc-containing yeast, HMBCa, L-ornithine hydrochloride, L-citrulline, magnesium carbonate, pullulan, aspartame and L-phenylalanine compound, sucralose, sodium glutamate, niacin, flavoring, calcium pantothenate, iron pyrophosphate, vitamin B6, L-arginine, glucosamine, folic acid, and vitamin B12; Maltose, barley leaf powder, chlorella raw powder, sensu gracilis powder, angelica powder, lactic acid bacteria powder, seaweed powder, spore-forming lactic acid bacteria, acai, avocado, maqui berry juice, dextrin, powdered rice husk, goji berry powder, acerola powder, pomegranate juice powder, amla extract, dragon fruit juice powder, rosehip extract, camu camu juice powder, gymnema sylvestre extract, salacia extract powder, garcinia extract powder, coleus extract, plant placenta, contains ceramide green juice containing pineapple extract, collagen peptide, prepared soy milk powder, isoflavone-containing soy extract, dried royal jelly powder, lycopene-containing tomato powder, citric acid, flavoring, vitamin C, aspartame / L-phenylalanine compound, acesulfame K, green tea extract, vitamin E, calcium pantothenate, vitamin B12, vitamin B2, vitamin B1, vitamin B6, hyaluronic acid, locust bean gum and β-carotene; A dietary nutritional powder containing at least one selected from cranberry powder, blueberry powder, and grape seed powder, and at least one selected from broccoli powder, pumpkin powder, and parsley powder; A meal powder containing an oil-in-water emulsion containing coconut oil and oat oil, barley grass flour, protein, water-soluble dietary fiber, vitamins, minerals, a sweetener, calcium carbonate, and an edible essence; A jelly containing grape juice, raspberry juice, hydrolyzed collagen, erythritol, pig-derived placenta extract, isoflavone, flavoring, vitamin C, calcium lactate, acesulfame K, aspartame-L-phenylalanine compound, thaumatin and flavonoids; Whey protein isolate, palatinose, defatted soybeans, psyllium husk powder, organic young barley leaf powder, calcium HMB, medium chain triglyceride, inulin, refined fish oil containing DHA and EPA, matcha, apple juice, cream extract powder containing milk ingredients, young barley leaf, kale, banana puree, broccoli, spore-forming lactic acid bacteria, fermented grain extract containing wheat, pumpkin, bok choy, parsley, carrot, celery, bitter melon, spinach, mulberry leaves, molokheiya, subtilis (Excluding meal replacement protein containing lead yeast, mugwort, tomato, copper yeast, flavoring, citric acid, tripotassium citrate, trisodium citrate, xanthan gum, vitamin C, acesulfame K, sucralose, magnesium oxide, quillaja extract, vitamin E, nicotinamide, calcium pantothenate, ferric pyrophosphate, vitamin B1, vitamin B6, vitamin A, vitamin B2, folic acid, vitamin D and vitamin B12).

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