Pipette tip devices and methods

Abstract

Methods and apparatus for attaching and detaching pipette tips to a pipettor and for storing pipette tips are provided. The pipette includes an aspiration block and one or more magnets secured to the aspiration block face. Reusable pipette tips have a pipette adapter with a magnetically attractable material. Pipetting devices are provided that include a pipettor magnetically attached to a pipette tip. Methods for using the above-described apparatus are also provided.

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Application No. 63 / 050,970, filed July 13, 2020, the entire contents of which are incorporated herein by reference.

[0002] The present disclosure relates generally to pipettors and pipette tips. The present disclosure also relates to methods and devices for installing, using, and removing pipette tips, and for storing pipette tips. [Background technology]

[0003] Pipettors are used in research laboratories and other environments to aspirate and dispense precise volumes of liquid, typically air-displacement pipettors. A wide variety of pipettors are available, each with a variety of liquid handling functions. A pipettor includes a syringe or other aspiration block coupled to a pipette tip. The syringe includes a piston that moves within a barrel. The pipette tip is coupled to the syringe so that a fluid path is established between the barrel and the pipette tip. The piston is driven, either manually or by a motor, to perform alternating forward and reverse strokes within the barrel. The forward stroke can be used to dispense liquid from the pipette tip, while the reverse stroke creates a suction force that can be used to aspirate liquid into the pipette tip. An automated pipetting device may include one or more pipettors, which may be part of a liquid handling apparatus that includes liquid containers with a variety of different liquids.

[0004] Disposable pipette tips have been used in pipettors for decades. Research laboratories may use thousands of pipette tips to aspirate and / or dispense samples and reagents in their various analytical procedures. When a laboratory is unable or unwilling to reuse pipette tips, the pipette tips are discarded after each use. The use of disposable pipette tips increases costs and labor for such laboratories because they must constantly purchase and replenish pipette tips and generate more waste from discarded pipette tips. As laboratories seek to increase the number of samples processed and analyzed, the costs and waste resulting from the disposal of pipette tips can become significant. Furthermore, there are many applications in which disposable pipette tips made of molded plastic do not have sufficient properties for the application. This increases the need to use more expensive, non-disposable tips made from metal, ceramic, etc. Non-disposable tips must be cleaned between handling different samples.

[0005] Pipette tips are available in many different sizes (e.g., 10 μL, 50 μL, 300 μL, 1 mL, 5 mL, etc.) and geometries, including both disposable and non-disposable types. Different procedures or protocols may require different pipette tips (size and / or type), and in some cases, the same procedure or protocol may require the use of different pipette tips. Some existing pipetting devices have the ability to accommodate different pipette tip styles but require the user to manually change pipette tips between automated procedures or protocols. Some existing pipetting devices have the ability to automatically change between disposable and non-disposable tips during a procedure or protocol, but the end of the pipette tip (which will be mated with a syringe) must have the same interface geometry. To accommodate disposable pipette tips sized to hold larger volumes (e.g., 1000 μL), the interface geometry can be designed around these larger tips. In this case, the connection to the pipettor used by smaller-volume disposable tips (e.g., 10 μL) is necessarily the same as that used by much larger tips, resulting in, for example, smaller-volume tips not being optimally designed for their small volume and use or application. For example, the connection to the smaller-volume tip may be much larger than desired. Furthermore, because the connection to the smaller-volume tip must be large (again, so that it can be interchangeable with a larger-volume tip coupled to the same pipettor), the smaller-volume tips cannot be placed next to each other in a grid with the relatively small spacing (e.g., 4.5 mm) required to access adjacent wells in a standard-sized multi-well (e.g., 384-well) plate. Furthermore, various procedures or protocols may require the use of multi-channel and / or single-channel pipetting devices.These two types of pipetting devices often have different mating surfaces, which may not allow the same size or type of pipette tip to be used in both multi-channel and single-channel pipetting devices.

[0006] In general, there has been a long-standing desire to increase the level of automation offered by liquid handling and pipetting devices to increase the throughput of such equipment and the "walk away" time available to users. In particular, there is a need to increase the level of compatibility between different types and sizes of pipette tips, as well as to automate the tasks of coupling pipette tips to syringes, uncoupling pipette tips from syringes, and swapping between different pipette tips in an automated manner. There is a need for an automated, highly reliable method for attaching, detaching, and storing pipette tips for reuse in the research process. Summary of the Invention

[0007] In one aspect of the present invention, a pipettor is provided, the pipettor comprising an aspiration block having an aspiration channel and an aspiration block end, the aspiration channel having an opening at the aspiration block end, and one or more magnets secured within the aspiration block at the aspiration block end.

[0008] In another aspect of the present invention, a reusable pipette tip is provided. The pipette tip includes a pipette adapter having an adapter proximal end, an adapter distal end, an adapter side, and an adapter lumen having openings at the adapter proximal end and the adapter distal end. The adapter side includes an adapter radial groove. The pipette adapter also includes a magnetically attractable material. The reusable pipette tip also includes a pipette tube having a pipette tube proximal end and a pipette tube distal end. The pipette tube proximal end is secured within the central lumen opening at the adapter distal end.

[0009] Another aspect of the present invention provides a pipetting device. The pipetting device includes a pipettor and a reusable pipette tip. The pipettor includes an aspiration block having an aspiration channel and an aspiration block end, the aspiration channel having an opening at the aspiration block end. The pipettor also includes one or more magnets secured within the aspiration block at the aspiration block end. The reusable pipette tip includes a pipette adapter having an adapter proximal end, an adapter distal end, adapter sides, and an adapter lumen with openings at the adapter proximal end and the adapter distal end. The adapter sides include an adapter radial groove. The pipette adapter includes a magnetically attractive material. The reusable pipette tip also includes a pipette tube having a pipette tube proximal end and a pipette tube distal end. The pipette tube proximal end is secured within a central lumen opening at the adapter distal end. The pipette tip is magnetically attached to the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and the pipette tip channel.

[0010] Another aspect of the present invention provides a method for using a reusable pipette tip in a pipettor. The method includes providing a pipettor having an aspiration block with an aspiration channel and an aspiration block end. The aspiration block also includes one or more magnets secured within the aspiration block at the aspiration block end. The method also includes positioning the aspiration block end sufficiently close to the reusable pipette tip to form a magnetic attachment. The reusable pipette tip includes a magnetically attractable material and a pipette adapter having an adapter proximal end, an adapter distal end, and an adapter lumen. The reusable pipette tip also includes a pipette tube having a pipette tube proximal end and a pipette tube distal end. The pipette tube proximal end is secured within a central lumen opening in the adapter distal end. The pipette tip is magnetically attached to the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and the pipette tip channel.

[0011] These and other advantages and features of the present apparatus and method will become apparent from the following detailed description, and from the appended claims. [Brief explanation of the drawings]

[0012] [Figure 1A] 1A-1C show an exemplary embodiment of a pipettor. [Figure 1B] 1A-1C show an exemplary embodiment of a pipettor. [Figure 2] 1A-1C show an exemplary embodiment of a pipetting device comprising a pipettor and reusable pipette tips. [Figure 3] 1A-1C are diagrams of exemplary embodiments of pipette tips magnetically attached to pipettors. [Figure 4] FIG. 10 is a diagram of another exemplary embodiment of a pipette tip magnetically attached to the aspiration block of a pipettor. [Figure 5] 1A and 1B are diagrams of an exemplary embodiment of a pipette tip magnetically attached to an aspiration block. [Figure 6] FIG. 10 is a diagram of another exemplary embodiment of a pipette tip magnetically attached to the aspiration block of a pipettor. [Figure 7] 1A and 1B are diagrams of exemplary embodiments of pipette tips magnetically attached to the aspiration block of a pipettor. [Figure 8A] FIG. 1 illustrates a pipetting system including a pipette tip storage device configured to hold one or more pipette tips. [Figure 8B] FIG. 1 illustrates a pipetting system including a pipette tip storage device configured to hold one or more pipette tips. DETAILED DESCRIPTION OF THE INVENTION

[0013] The present teachings are best understood from the following detailed description when read in conjunction with the accompanying drawing figures, the features of which are not necessarily drawn to scale.

[0014] Some embodiments of the present devices and methods provide for easy and quick attachment and detachment of reusable pipettes to pipettors. In some embodiments, among other advantages, pipette tips are held securely in place during use, the seal between the pipette adapter and the pipettor's aspiration block is substantially airtight and liquid-tight, and dead volume is minimized. Some embodiments of the pipetting system allow pipette tips to be removed from a pipette tip storage location and returned to the same location or a different location if desired. In some embodiments, the magnetic attachment mechanism employed in the present devices can accommodate possible errors in the location of pipette tips relative to the pipette tip storage device, including, for example, + / - 0.75 mm in the X and Y directions when the pipette tip is placed in the storage device.

[0015] In one aspect, the novel pipettor comprises an aspiration block and one or more magnets. The magnets are fixed to the aspiration block end of the aspiration block. In some embodiments, the magnets are recessed from the aspiration block face so that they do not physically contact the pipette tip when the pipette tip is magnetically attached to the pipettor. Any suitable magnets can be used, including small but powerful neodymium-iron-boron magnets, and can be positioned within the aspiration block face. The aspiration block may be comprised in whole or in part of stainless steel or other materials and may include tubing or other aspiration channels. The magnets should be strong enough to pick up and securely hold the pipette tip. In some embodiments, the magnets are strong enough to compress a compliant material positioned to form a seal between the aspiration block and the pipette tip.

[0016] In some embodiments, the pipettor is adapted to transfer fluids by pipetting, including, for example, aspirating or dispensing volumes of fluids from 10 μl to 500 μl. The pipettor can also be adapted to mix fluids in a mixing strip, change pipette tips, detect liquid levels, and deliver water or solvent to a slide processing module, humidifier pad, or mixing strip to adjust wettability. In some embodiments, the pipettor includes one or more features adapted to clean the inside of a pipette tip. The pipettor can be connected to one or more, or all, of a processing module, a washing station, a pipette tip storage station, a reagent vial storage, and a gantry.

[0017] The present methods and devices may include a pipettor having a pipette tip. In some embodiments, the pipette tip is a disposable or reusable pipette tip. Reusable pipette tips require cleaning between uses to avoid contamination. It is contemplated that the present methods and devices may optionally be used with disposable pipette tips, thereby reducing the cost and waste associated with replacing pipette tips after a single use.

[0018] FIG. 1A illustrates an exemplary embodiment of a pipettor. The pipettor 100 includes an aspiration block 102 and a piston 104 that controls the movement of fluid into and out of the aspiration block 102. The piston 104 can be actuated by any suitable mechanism. In FIG. 1A, it is actuated by a motor 106 that rotates a lead screw 108, which moves a stage 112, thereby moving the piston 104 up and down. The piston 104 moves up to aspirate fluid into the aspiration block and down to dispense fluid from the pipettor. The pipettor 100 also includes a valve pack 110 to control which fluid is delivered to the aspiration block 102. In some embodiments, the pipettor is configured to deliver at least two types of liquid to the pipette tip. For example, the pipettor can be configured to deliver one or more reagents or wash fluids. The aspiration block can deliver pressurized air or other gas to the interior of the tip. Pressurized gas can be delivered at two different flow rates (low and high) to perform different steps or functions.

[0019] FIG. 1B provides another view of the aspiration block 102. One or more liquids and / or one or more pressurized gases may enter the aspiration block 102 through conduits, valves, or other pathways. In FIG. 1B, a valve pack 110 controls the delivery of reagents, wash fluids, pressurized gases, and other fluids (e.g., solvents and buffers) to the aspiration block 102. As fluids are delivered through the aspiration block 102, they pass through its outlet 114 to an attached pipette tip. The outlet 114 of the aspiration block 102 may include a tapered cone 116 to facilitate pipette tip attachment.

[0020] In some embodiments, pipette tips are attached to the pipettor using one or more magnets 120, e.g., six magnets arranged in a circle, that are affixed to the aspiration block by adhesive or the like. The device may include a seal (e.g., an O-ring seal) between the pipette tip and the nipple of the aspiration block of the pipettor. The tapered cone 116 may have a recess for an O-ring or other seal. A pipette tip sensor 118 may be positioned to detect whether a pipette tip is present or attached to the aspiration block 102 and provide a signal to the controller to enable or stop fluid flow from the pipettor to the outlet 114.

[0021] In some embodiments, the pipettor can be automatically moved along one or more axes (X, Y, and / or Z) by operation of a gantry or other robotic device. Gantries and other devices can be positioned to move the pipettor horizontally and vertically to change pipette tips, such as by moving up and down to separate a first pipette tip and attach a second pipette tip.

[0022] 2 shows the interior of an exemplary embodiment of a pipetting device comprising a pipettor 200 and a reusable pipette tip 201. The pipettor 200 comprises an aspiration block 202 comprising an aspiration channel 222 and an aspiration block end 224, which has an opening (outlet 214) at the aspiration block end 224. The pipettor 200 also comprises one or more magnets 220 fixed within the aspiration block 202 at the aspiration block end 224.

[0023] The pipetting device also includes a reusable pipette tip 201 that includes a pipette adapter 203 and a pipette tube 215. The pipette adapter 203 includes an adapter proximal end 205, an adapter distal end 207, and an adapter side 209. The pipette adapter 203 also includes an adapter lumen 211 that has openings at the adapter proximal end 205 and the adapter distal end 207. The adapter side 209 of the pipette adapter 203 includes an adapter radial groove 213. The pipette adapter 203 includes, for example, is formed from, or contains an amount of, a magnetically attractive material. The pipette tube 215 of the reusable pipette tip 201 includes a pipette tube proximal end 217 and a pipette tube distal end 219. The pipette tube proximal end 215 is secured within the adapter lumen 211, which opens at the adapter distal end 207. The pipette tip 201 is magnetically attached to the pipettor 200 to provide a substantially fluid-tight flow path between the aspiration channel 222 and the pipette tip channel 221 .

[0024] In another aspect, a reusable pipette tip is provided having a pipette adapter including a magnetically attractable material. The pipette adapter further comprises an adapter proximal end, an adapter distal end, an adapter side, and an adapter lumen. In some embodiments, the adapter side comprises an adapter radial groove. The reusable pipette tip also comprises a pipette tube having a pipette tube proximal end and a pipette tube distal end. The pipette tube proximal end is secured within a central lumen opening in the adapter distal end.

[0025] FIG. 3 is a cross-sectional view of a portion of a pipette tip 301 magnetically attached to the aspiration block 302 of a pipettor. The reusable pipette tip 301 includes a pipette adapter 303 with an adapter proximal end 305, an adapter distal end 307, and an adapter side 309. The adapter radial groove 313 can facilitate storage of the pipette tip 301 by providing a feature that can engage with a storage device. A compliant material 323 can facilitate placement of the pipette tip 301 within the storage device, compensating for slight deviations from the expected position of the adapter radial groove 313 upon engagement within the storage device, enabling its versatility. The pipette adapter 303 also includes an adapter lumen 311 with openings at the adapter proximal end 305 and the adapter distal end 307. The adapter side 309 includes the adapter radial groove 313 with the compliant material 323 within the adapter auxiliary groove 325. Pipette adapter 303 includes a magnetically attractive material, and suction block 302 includes a magnet 320 recessed from suction block face 324 facing adapter proximal end 305 .

[0026] In some embodiments, the aspiration block 302 also includes an extension 326 through which the aspiration channel 322 extends, thereby providing an extended outlet. The extension 326 is adapted to fit within an opening 327 of the pipette tip 301, such that the opening 327 of the pipette tip 301 is in fluid communication with the aspiration channel 322. In some embodiments, the extension 326 includes a tapered cone 328 extending from the aspiration block face 324, with the aspiration channel 322 extending through the tapered cone 328.

[0027] In some embodiments, the adapter lumen 311 of the pipette adapter 303 includes an opening at the adapter distal end 307 configured to receive a pipette tube, into which the pipette tube can be secured. The pipette tip 301 is magnetically attached to the aspiration tube 302 to provide a substantially fluid-tight flow path between the aspiration channel 322 and the pipette tube within the adapter lumen 311.

[0028] In some embodiments, the pipette adapter 303 further comprises an adapter cup formed by an adapter proximal end 305 and an adapter cup wall 337 extending from the adapter proximal end 305. The adapter cup wall may surround part of or all of the adapter proximal end 305. The adapter cup wall 337 may also comprise a cup wall guide 339 opposite the adapter proximal end 305. For example, the cup wall guide 339 may be a sloped surface. The adapter cup wall 337 may further comprise an inner surface 341 and one or more radial rings 343 on the inner surface 341 facing toward the center.

[0029] FIG. 4 shows an internal view of another embodiment of a pipette tip 401 magnetically attached to the suction block 402 of a pipettor. The reusable pipette tip 401 includes a pipette adapter 403 having an adapter proximal end 405, an adapter distal end 407, and an adapter side 409. The pipette adapter 403 also includes an adapter lumen 411 with openings at the adapter proximal end 405 and the adapter distal end 407. The adapter side 409 includes an adapter radial groove 413. Unlike the pipette tip 301 of FIG. 3, the pipette adapter 403 does not include a compliant material within the radial groove 413. Such an embodiment would be useful if the storage device could compensate for slight displacement of the pipette tip from its expected position during storage. The pipette adapter 403 includes a magnetically attractable material, and the suction block 402 includes a magnet 420 recessed from the suction block face 424 facing the adapter proximal end 405.

[0030] The aspiration block 402 also includes an extension 426 that extends into an opening 427 in the pipette adapter 403. The extension 426 includes a tapered cone 428 extending from the aspiration block face 424. The opening 427 includes a tapered opening 429 configured to receive the tapered cone 428. The pipette adapter 403 further includes an opening groove 431 at the bottom of the tapered opening 429 and a compliant sealant 433, such as an O-ring, within the opening groove 431. The inclusion of the compliant sealant 433 provides a radial-type seal between the aspiration tube 402 and the pipette adapter 403. The radial seal should be substantially liquid-tight.

[0031] 5 is a cross-sectional view of yet another embodiment of a pipette tip 501 magnetically attached to an aspiration block 502 of a pipettor. The reusable pipette tip 501 includes a pipette adapter 503 having an adapter proximal end 505, an adapter distal end 507, and an adapter side 509. The pipette adapter 503 also includes an adapter lumen 511 having openings at the adapter proximal end 505 and the adapter distal end 507. The pipette tip 501 is magnetically attached to an aspiration tube 502 to provide a substantially fluid-tight flow path between the aspiration channel 522 and the pipette tube within the adapter lumen 511.

[0032] The adapter side 509 includes an adapter radial groove 513 with a compliant material 523 in an adapter auxiliary groove 525. The pipette adapter 503 includes a magnetically attractable material, and the aspiration block 502 includes a magnet 520 recessed from an aspiration block face 524 facing the adapter proximal end 505. The aspiration tube 502 and pipette adapter 503 of FIG. 5 differ from those shown in FIGS. 3 and 4 in that the aspiration tube 502 does not include a tapered cone and the pipette adapter 503 does not include a tapered opening. The end of the aspiration block 502, i.e., the aspiration block face 524, is substantially flat and faces the substantially flat adapter proximal end 505. The magnet 520 is slightly recessed from the aspiration block face 524 to prevent the magnet from physically contacting the pipette adapter 503.

[0033] The adapter proximal end 505 includes an adapter proximal end groove 535 and a compliant seal 533 (e.g., an O-ring) within the adapter proximal end groove 535, which provides a static face seal between the aspiration tube 502 and the pipette adapter 503. In some embodiments, the pipette adapter 503 further includes an adapter cup formed by the adapter proximal end 505 and an adapter cup wall 537 extending from the adapter proximal end 505. The adapter cup wall 537 also includes a cup wall guide 539 on the opposite side of the adapter proximal end 505. The adapter cup wall 537 in FIG. 5 includes two radial rings 543 on its inner surface 541 that engage the aspiration block 502. In this embodiment, tightly toleranced radial rings 543 are used at the top and bottom of the adapter cup to align the pipette adapter 503. Two radial rings 543, which may be spaced apart by, for example, about 15 mm, are advantageous for controlling concentricity and tilt.

[0034] 6 shows a cross-sectional view of yet another embodiment of a pipette tip 601 magnetically attached to an aspiration block 602 of a pipettor. The reusable pipette tip 601 includes a pipette adapter 603 including an adapter proximal end 605, an adapter distal end 607, and an adapter side 609. The adapter side 609 includes an adapter radial groove 613 having a compliant material 623. The pipette adapter 603 includes a magnetically attractive material, and the aspiration block 602 includes a magnet 620 recessed from an aspiration block face 624 facing the adapter proximal end 605. The adapter proximal end 605 includes a compliant seal 633 (e.g., an O-ring) in an adapter proximal end groove 635.

[0035] The pipette adapter 603 of Figure 6 further includes an adapter cup formed by an adapter proximal end 605 and an adapter cup wall 637 extending from the adapter proximal end 605. The pipette adapter 603 of Figure 6 differs from that shown in Figure 5 in that the adapter cup wall 637 includes only one radial ring 643 and the adapter cup wall 637 has a relatively low height. The adapter proximal end 605 also includes rounded or chamfered edges.

[0036] 7 is a cross-sectional view of an exemplary embodiment of a pipette tip 701 magnetically attached to an aspiration block 702 of a pipettor. The reusable pipette tip 701 includes a pipette adapter 703 including an adapter proximal end 705, an adapter distal end 707, and an adapter side 709. The pipette adapter 703 also includes an adapter lumen 711 having openings at the adapter proximal end 705 and the adapter distal end 707. The adapter side 709 includes an adapter radial groove 713, which may or may not include a compliant material and an adapter auxiliary groove. The pipette adapter 703 includes a magnetically attracting material, and the aspiration block 702 includes a magnet 720 recessed from an aspiration block face 724 facing the adapter proximal end 705.

[0037] In some embodiments, the aspiration block 702 also includes an extension 726 through which the aspiration channel 722 extends. The extension 726 is adapted to fit within an opening 727 of the pipette tip 701 such that the opening 727 of the pipette tip 701 is in fluid communication with the aspiration channel 722. In some embodiments, the extension 726 includes a tapered cone 728 extending from the aspiration block face 724, the opening 727 including a tapered opening 729, and the aspiration channel 722 extending through the tapered cone 728.

[0038] In some embodiments, the adapter lumen 711 of the pipette adapter 703 includes an opening at the adapter distal end 707 configured to receive a pipette tube 715, which can be secured therein. The pipette tip 701 is magnetically attached to the aspiration tube 702 to provide a substantially fluid-tight flow path between the aspiration channel 722 and the pipette tube 715 within the adapter lumen 711.

[0039] In some embodiments, the pipette adapter 703 further comprises an adapter cup formed by an adapter proximal end 705 and an adapter cup wall 737 extending from the adapter proximal end 705. The adapter cup wall 737 does not surround or enclose the suction block face 724. Instead, the adapter proximal end 705 has a size equal to or smaller than the outer periphery of the suction block face 724. For example, the adapter proximal end 705 and the suction block face 724 each have an outer periphery, e.g., a circumference, and the outer periphery of the adapter proximal end 705 is equal to or smaller than the outer periphery of the suction block face 724. The adapter cup wall 737 may also comprise a cup wall top 745 that faces the suction block face 724.

[0040] In another aspect, a method for using a reusable pipette tip with a pipettor is provided. The method may include providing a pipettor having an aspiration block with an aspiration channel and an aspiration block end. The aspiration channel has an opening at the aspiration block end. The aspiration block also includes one or more magnets secured within the aspiration block at the aspiration block end. The method may also include positioning the aspiration block end sufficiently close to the reusable pipette tip to form a magnetic attachment. The reusable pipette tip includes a pipette adapter having a magnetically attractable material, an adapter proximal end, an adapter distal end, and an adapter lumen. The reusable pipette tip also includes a pipette tube having a pipette tube proximal end and a pipette tube distal end. The pipette tube proximal end is secured within a central lumen opening at the adapter distal end. The pipette tip is magnetically attached to the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and the pipette tip channel.

[0041] In some embodiments, where the pipette adapter further comprises adapter sides, the adapter sides comprising adapter radial grooves, the method may also include moving the pipettor and the magnetically attached pipette tip to a storage device adapted to receive the pipette adapter and transferring the pipette adapter to the storage device. In some embodiments, where the storage device comprises an empty storage fork having storage fork prongs separated by a storage fork mouth, the pipettor is moved such that the magnetically attached pipette tip is inserted into the storage fork mouth in a direction substantially parallel to the storage fork prongs. The method may also include moving the pipettor away from the engaged pipette in a direction substantially perpendicular to the storage fork prongs, thereby overcoming the magnetic attraction between the magnet in the aspiration body and the pipette adapter while the storage fork prongs prevent the inserted adapter from disengaging from the storage fork.

[0042] In some embodiments, the storage device further includes a second storage fork that holds a second pipette tip. The method further includes moving the pipettor so that the aspiration channel is positioned above the second pipette tip held by the storage fork, moving the pipettor toward the second pipette tip to magnetically attach the pipettor to the second pipette tip, and moving the pipettor in a direction substantially parallel to the storage fork blades, thereby removing the second pipette tip from the storage device. The method may also include detecting whether the second pipette tip is attached to the aspiration channel before removing the second pipette tip from the storage device.

[0043] The present methods and apparatus may further include, or be part of, a method or system for preparing a sample for analysis. Such a system may include other devices for performing other functions. For example, the pipettor may be mounted on a gantry for automating movement between various locations (e.g., between a pipette tip storage location and a location for pipette use (e.g., a sample processing location, a reagent aspiration location, etc.)). In some embodiments, the present methods and apparatus are included in a liquid handling apparatus that further includes one or more of a reagent source, a sample processing module, a pipette washing device, or other devices.

[0044] 8A and 8B show a pipetting system 800 including a pipette tip storage device 801 configured to hold one or more pipette tips. The storage device 800 includes multiple storage forks 802, 804 configured to engage and hold pipette adapters 803, 805, 807. In some embodiments, the storage forks 802, 804 are configured to receive the pipette adapters 803, 805 in their adapter radial grooves. The storage forks 802, 804 are mounted on a storage block 806. The pipette adapter 807 is magnetically attached at an aspiration block connection 808, which is held by an aspiration mounting block 810 on a base 812 of the system 800. The system 800 may also include a gantry 814 that can move the aspiration mounting block 810 to the pipette tip storage device 801 and / or to other devices or locations for pipetting use in the system 800 (e.g., sample processing locations or devices, reagent aspiration locations or devices, etc.).

[0045] 8B provides a close-up view of storage forks 802, 804 for holding one or more pipette tips. The storage forks 802, 804 are configured to engage and hold the adapter radial grooves of a pipette adapter for reusable pipette tips. Each storage fork includes storage fork tines separated by a storage fork mouth, and a storage fork may include two sets of storage forks and storage fork mouths. For example, storage fork 802 includes a pair of storage fork tines 820, 822 separated by storage fork mouth 821 and another pair of storage fork tines 830, 832 separated by storage fork mouth 831. The storage fork tines 830, 832 are configured to receive the adapter radial groove 813 into the storage fork mouth 831 in a direction substantially parallel to the storage fork tines 830, 832 and to prevent the inserted pipette adapter 803 from disengaging from the storage fork 802 in a direction substantially perpendicular to the storage fork tines 830, 832. The storage fork mouth 831 is wider at the adapter radial groove 813 than the pipette adapter and narrower than the adapter side 809 adjacent to the adapter radial groove 813.

[0046] The storage fork can be fixedly or pivotally mounted on the storage block. For example, the storage fork 802 is fixedly mounted on the storage block 806 by a fixture 836. The position of the pipette adapter 803 can be established and compensated for within the rigid storage fork mouth 831 by an O-ring in the adapter radial groove 813. The installation diameter of the pipette adapter is smaller than the outer diameter of the O-ring. As another example, the storage fork 804 is pivotally mounted on the storage block 806 by a single fixture 834 in the center of the storage fork 804. The pipette adapter 805 extends into the storage fork 804, but there is no O-ring and its installation diameter is a tight fit with the fork. The pivoting ability of the storage fork 804 allows the system to accommodate misalignment of the pipette tip in the X or Y direction. The storage fork 804 includes a magnet 837 for attracting the pipette adapter 805 into the storage fork 804. The storage fork 804 also includes a spring plunger 838 to resist the horizontal magnet 837 and hold the pipette adapter 804 in its nominal position. In some embodiments, the storage device includes one or more magnets aligned with opposite poles of the magnet in the storage fork.

[0047] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting. Defined terms are given the technical and scientific meaning as commonly understood and accepted in the art of the present teachings of the defined terms.

[0048] As used herein, the term "block" or "body" generally includes any structure comprising one or more channels, such as by channels formed in the block or body. In some embodiments, the block or body comprises multiple channels, where separate fluids can flow through the block or body. In some embodiments, the block or body comprises a manifold in communication with one or more internal flow paths and / or one or more external flow paths.

[0049] In some embodiments of the device, the aspiration block, pipette adapter, or other component comprises a channel or tube. The terms "channel" and "tube" generally include any structure configured to define a flow path for fluid movement. Channels and tubes typically have openings at first and second ends, which may also be referred to as inlets and outlets in the context of the intended flow direction. A channel or tube may have two or more inlets and / or two or more outlets. In some embodiments, a channel is formed in a larger body or block. In some embodiments, a tube is a conduit internal or external to another component. The geometry of a channel or tube can vary widely, including circular, rectangular, square, D-shaped, trapezoidal, or other polygonal cross-sections.

[0050] In some embodiments described above, the surface or connecting portion comprises a compliant material, such as a substantially fluid-impermeable compliant material. In some embodiments, the compliant material is in the form of an O-ring. The compliant material can be of any suitable shape or composition. In some embodiments, the compliant material can be a fluoroelastomer material. The compliant material can be various rubbers, such as fluoropolymer, Buna-n, or EPDM, depending on the fluid used in the device. Grooves or other features in the aspiration block, pipette adapter, or other structure can align with the compliant material and help retain it on the body. Groove depth can be specified to determine how much the compliant material must compress to perform a desired function, such as forming a fluid-tight seal.

[0051] In some embodiments, the devices and methods may be configured to move a pipettor or aspiration block between two or more pipettor positions, and the pipettor may be changed or switched from one pipettor position to another by manually operated movements, including linear or translational movements, rotational movements, or a combination thereof.

[0052] In some embodiments, the present apparatus and methods may include a controller, such as a data processing unit, a conventional PC, or a workstation. The controller may be connected to one or more of the present devices to receive information and / or control operation. For example, the controller may control the operation of the pipettor and receive information about the actual operating conditions therefrom. The controller may also control the operation of the gantry and receive information about the actual operating conditions therefrom (flow rate, vacuum level, etc.). The controller may further control the operation of other devices.

[0053] In this disclosure, the terms "substantial" or "substantially" mean within the limits or degree acceptable to those of ordinary skill in the art. The terms "approximately" and "about" mean within the limits or amount acceptable to those of ordinary skill in the art. The term "about" generally refers to plus or minus 15% of the indicated number. For example, "about 10" may indicate a range of 8.5 to 11.5. For example, "approximately the same" means that the items being compared would be considered the same by a person of ordinary skill in the art. When a range of values ​​is described in this disclosure, it should be understood that every intermediate value between the upper and lower limits of that range, to the tenth of the unit of the lower limit, unless the context clearly dictates otherwise, is also expressly disclosed. When the stated range includes one or both of the limits, the invention also includes ranges excluding either or both of them.

[0054] As used herein and in the appended claims, the terms "a," "an," and "the" include both singular and plural referents unless the context clearly dictates otherwise. For example, "a conduit" includes one conduit and multiple conduits. Unless otherwise indicated, the terms "first," "second," "third," and other ordinal numbers are used herein to distinguish between different elements of the devices and methods, but are not intended to imply numerical limitations. Reference to first and second pipettor positions should not be construed to mean that the apparatus has only two pipettor positions. A device having first and second elements may also include thirds, fourths, fifths, etc., unless otherwise indicated.

[0055] Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present teachings, some exemplary methods and materials are described below. All patents and publications mentioned herein are expressly incorporated by reference in their entirety.

[0056] References Powell et al. U.S. Patent No. 8,168,137 Sheldon U.S. Patent No. 9,079,178

[0057] Illustrative Embodiments Exemplary embodiments provided in accordance with the presently disclosed subject matter include, but are not limited to, the following.

[0058] Embodiment 1 A pipetter comprising an aspiration block having an aspiration channel and an aspiration block end, the aspiration channel having an opening at the aspiration block end, and one or more magnets fixed within the aspiration block at the aspiration block end.

[0059] Embodiment 2 2. A pipettor as described in embodiment 1, wherein the aspiration block end comprises an aspiration block surface configured to be magnetically attached to a pipette tip.

[0060] Embodiment 3 3. The pipettor of embodiment 2, wherein the one or more magnets are recessed from the suction block face.

[0061] Embodiment 4 4. A pipettor according to any one of embodiments 1 to 3, wherein the one or more magnets comprise neodymium iron boron magnets.

[0062] Embodiment 5 5. A pipettor according to any one of embodiments 1 to 4, wherein the one or more magnets comprise four or more magnets arranged in a circle around the suction channel.

[0063] Embodiment 6 A pipettor as described in any one of embodiments 1 to 5, wherein the suction block end has an extension through which the suction channel extends, the extension adapted to fit within the opening of the pipette tip, such that the opening of the pipette tip is in fluid communication with the suction channel.

[0064] Embodiment 7 7. A pipettor as described in embodiment 6, wherein the extension comprises a tapered cone extending from the aspiration block face, the aspiration channel extending through the tapered cone.

[0065] Embodiment 8 8. A pipettor according to any one of embodiments 1 to 7, further comprising a pipette tip sensor within the aspiration block end configured to detect whether a pipette tip is attached to the pipettor.

[0066] Embodiment 9 1. A pipette adapter comprising: an adapter proximal end, an adapter distal end, an adapter side, and an adapter lumen having openings at the adapter proximal end and the adapter distal end, the adapter side includes an adapter radial groove; The pipette adapter includes a magnetically attractable material; a pipette tube having a pipette tube proximal end and a pipette tube distal end; The proximal end of the pipette tube is secured within a central lumen opening at the distal end of the adapter; Reusable pipette tips.

[0067] Embodiment 10 10. A reusable pipette tip as described in embodiment 9, wherein the pipette adapter is cylindrical and the adapter radial groove completely surrounds the side of the adapter.

[0068] Embodiment 11 11. A reusable pipette tip according to embodiment 9 or 10, wherein the adapter radial groove has a diameter between 6.10 mm and 5.90 mm, a height between 1.60 mm and 1.40 mm, and a distance from the top surface of the pipette adapter between 16.89 mm and 16.87 mm.

[0069] Embodiment 12 12. A reusable pipette tip according to any one of embodiments 9 to 11, further comprising a compliant material within the adapter radial groove.

[0070] Embodiment 13 13. A reusable pipette tip as described in embodiment 12, wherein the adapter radial groove comprises an adapter auxiliary groove configured to receive a portion of the extensible material.

[0071] Embodiment 14 14. A reusable pipette tip according to any one of embodiments 9 to 13, wherein the magnetically attracting material is selected from stainless steel, 17-4 PH (heat treatment condition H900), and combinations thereof.

[0072] Embodiment 15 15. A reusable pipette tip according to any one of embodiments 9 to 14, wherein the adapter proximal end has rounded or chamfered edges.

[0073] Embodiment 16 A reusable pipette tip as described in any one of embodiments 9 to 15, wherein the pipette adapter further comprises an adapter cup formed by an adapter proximal end and an adapter cup wall extending from the adapter proximal end.

[0074] Embodiment 17 17. A reusable pipette tip as described in embodiment 16, wherein the adapter cup wall partially or completely surrounds the adapter proximal end.

[0075] Embodiment 18 18. A reusable pipette tip according to any one of embodiments 9 to 17, wherein the adapter cup wall comprises a cup wall guide opposite the adapter proximal end, the cup wall guide comprising an inclined surface.

[0076] Embodiment 19 19. A reusable pipette tip according to any one of embodiments 9 to 18, wherein the adapter cup wall comprises an inner surface and a radial ring on the inner surface facing the center.

[0077] Embodiment 20 20. A reusable pipette tip as described in embodiment 19, wherein the adapter cup wall comprises only one radial ring.

[0078] Embodiment 21 21. A reusable pipette tip according to embodiment 19 or 20, wherein the adapter cup wall has a height between 4.01 mm and 3.99 mm.

[0079] Embodiment 22 22. A reusable pipette tip according to any one of embodiments 9 to 21, wherein the adapter proximal end comprises a tapered opening surrounding a central lumen.

[0080] Embodiment 23 23. A reusable pipette tip as described in embodiment 22, wherein the tapered opening comprises an opening groove and a compliant sealant within the opening groove.

[0081] Embodiment 24 24. A reusable pipette tip according to any one of embodiments 9 to 23, wherein the adapter proximal end comprises an adapter proximal end groove and a compliant sealant within the adapter proximal end groove.

[0082] Embodiment 25 (a) A pipettor, a suction block having a suction channel and an end of the suction block, the suction channel having an opening at the end of the suction block; a pipettor comprising one or more magnets fixed within the aspiration block at an end of the aspiration block; (b) a reusable pipette tip, a pipette adapter comprising an adapter proximal end, an adapter distal end, an adapter side, and an adapter lumen having openings at the adapter proximal end and the adapter distal end; the adapter side includes an adapter radial groove; The pipette adapter comprises a magnetically attractable material; and a pipette tube having a pipette tube proximal end and a pipette tube distal end; The proximal end of the pipette tube is fitted with a reusable pipette tip that is secured within the central lumen opening at the distal end of the adapter. Equipped with The pipette tip is magnetically attached to the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and the channel of the pipette tip. Pipetting device.

[0083] Embodiment 26 26. A pipetting device according to embodiment 25, wherein the aspiration block end is substantially flat.

[0084] Embodiment 27 27. A pipetting device according to embodiment 25 or 26, wherein the one or more magnets are slightly recessed from the suction block face so that the one or more magnets do not come into physical contact with the pipette adapter.

[0085] Embodiment 28 28. A pipetting device according to any one of embodiments 25 to 27, wherein the pipette adapter further comprises an adapter cup formed by an adapter proximal end and an adapter cup wall extending from the adapter proximal end.

[0086] Embodiment 29 29. A pipetting device as described in embodiment 28, wherein the adapter cup wall has a radial ring configured to contact and align the pipette adapter with the aspiration channel.

[0087] Embodiment 30 30. A pipetting device as described in embodiment 28 or 29, wherein the adapter cup wall comprises a cup wall guide opposite the adapter proximal end, the cup wall guide comprising an inclined surface for guiding the suction channel into the adapter cup.

[0088] Embodiment 31 28. A pipetting device according to any one of embodiments 25 to 27, wherein the adapter proximal end has a size equal to or smaller than the aspiration block end.

[0089] Embodiment 32 A pipetting device described in any one of embodiments 25 to 27 or embodiment 31, wherein the adapter proximal end and the suction block face each have an outer periphery, and the outer periphery of the adapter proximal end is equal to or smaller than the suction block face.

[0090] Embodiment 33 A pipetting device according to any one of embodiments 25 to 32, and a storage device configured to hold one or more of the pipette tips by engaging the adapter radial groove of the pipette tip; 1. A reusable pipette tip system comprising:

[0091] Embodiment 34 the storage device comprises a plurality of storage forks, each of the storage forks having storage fork tines separated by a storage fork jaw; A system as described in embodiment 33, wherein the storage fork is configured to receive the adapter radial groove into the storage fork mouth in a direction substantially parallel to the storage fork blade and to prevent the inserted adapter from detaching from the storage fork in a direction substantially perpendicular to the storage fork blade.

[0092] Embodiment 35 35. The system of embodiment 34, wherein the storage fork mouth is wider at the adapter radial groove than the adapter and narrower than the adapter side adjacent to the adapter radial groove.

[0093] Embodiment 36 A system described in any one of embodiments 33 to 35, wherein the storage device comprises a storage body to which a storage fork is attached.

[0094] Embodiment 37 37. The system of embodiment 36, wherein the storage fork is fixedly mounted on the storage block.

[0095] Embodiment 38 38. The system of embodiment 37, wherein each of the storage forks comprises one or more spring plungers arranged to press against an adapter radial groove of a pipette adapter inserted into the storage fork.

[0096] Embodiment 39 37. The system of embodiment 36, wherein the storage fork is fixedly mounted on the storage block.

[0097] Embodiment 40 40. A system according to any one of embodiments 34 to 39, wherein the storage fork comprises one or more magnets arranged to magnetically attract the pipette device.

[0098] Embodiment 41 41. The system of embodiment 40, wherein the storage device comprises one or more magnets aligned with opposite magnetic poles of the one or more magnets in the storage fork.

[0099] Embodiment 42 1. A method of using reusable pipette tips in a pipettor, comprising: providing a pipetter comprising an aspiration block having an aspiration channel and an aspiration block end, the aspiration channel having an opening at the aspiration block end, and one or more magnets fixed within the aspiration block at the aspiration block end; placing the end of the aspiration block close enough to form a magnetic attachment from the reusable pipette tip; Including, Reusable pipette tips a pipette adapter comprising a magnetically attractable material, an adapter proximal end, an adapter distal end, and an adapter lumen having openings at the adapter proximal end and the adapter distal end; a pipette tube having a pipette tube proximal end and a pipette tube distal end; The proximal end of the pipette tube is secured within a central lumen opening at the distal end of the adapter; The method wherein the pipette tip is magnetically attached to the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and the channel of the pipette tip.

[0100] Embodiment 43 the pipette adapter further comprises an adapter side, the adapter side comprising an adapter radial groove; moving the pipettor and magnetically attached pipette tip to a storage device adapted to receive a pipette adapter; 43. The method of embodiment 42, further comprising transferring the pipette adapter to a storage device.

[0101] EMBODIMENT 44 the storage device comprises an empty storage fork with storage fork tines separated by a storage fork mouth, the pipettor being moved such that the magnetically attached pipette tip is inserted into the storage fork mouth in a direction substantially parallel to the storage fork tines; 44. The method of embodiment 43, wherein the pipettor is moved away from the engaged pipette in a direction substantially perpendicular to the storage fork blade, and the magnetic attraction between the one or more magnets in the suction body and the pipette adapter is overcome while the storage fork blade prevents the inserted adapter from detaching from the storage fork.

[0102] Embodiment 45 the storage device further comprises a second storage fork for holding a second pipette tip; moving the pipettor so that the aspiration channel is positioned above a second pipette tip held by the storage fork; moving the pipettor toward a second pipette tip to magnetically attach the pipettor to the second pipette tip; 44. The method of embodiment 43, further comprising moving the pipettor in a direction substantially parallel to the storage fork blade, thereby removing the second pipette tip from the storage device.

[0103] Embodiment 46 46. ​​The method of embodiment 45, further comprising detecting whether the second pipette tip is attached to the aspiration channel before removing the second pipette tip from the storage device.

[0104] The above description of exemplary or preferred embodiments should be construed as illustrating, rather than limiting, the invention as defined by those embodiments. As will be readily appreciated, numerous variations and combinations of the features described above can be utilized without departing from the invention as described in the embodiments. Such variations are not to be considered a departure from the scope of the invention, and all such variations are intended to be included within the scope of the following embodiments. All references cited herein are incorporated by reference in their entirety.

Claims

1. 1. A pipette adapter comprising: an adapter proximal end, an adapter distal end, an adapter side, and an adapter lumen having openings at the adapter proximal end and the adapter distal end, the adapter side portion includes an adapter radial groove; the pipette adapter comprises a magnetically attracting material; a pipette tube having a pipette tube proximal end and a pipette tube distal end; A reusable pipette tip comprising: a proximal end of the pipette tube is secured within the central lumen opening at the distal end of the adapter to form the reusable pipette tip; The reusable pipette tip is configured to be holdable by a storage fork of a storage device for holding pipette tips by receiving and engaging the storage fork with the adapter radial groove.

2. 2. The reusable pipette tip of claim 1, wherein the pipette adapter is cylindrical and the adapter radial groove completely surrounds the side of the adapter.

3. 3. The reusable pipette tip of claim 1, wherein the adapter radial groove has a diameter between 6.10 mm and 5.90 mm, a height between 1.60 mm and 1.40 mm, and a distance from the top surface of the pipette adapter between 16.89 mm and 16.87 mm.

4. 4. The reusable pipette tip of claim 1, further comprising a compliant material within the adapter radial groove.

5. The reusable pipette tip of claim 4 , wherein the adapter radial groove comprises an adapter auxiliary groove configured to receive a portion of the compliant material.

6. 6. The reusable pipette tip of claim 1, wherein the magnetically attractive material is selected from stainless steel, 17-4 PH (heat treatment condition H900), and combinations thereof.

7. 7. The reusable pipette tip of claim 1, wherein the adapter proximal end comprises rounded or chamfered edges.

8. 8. The reusable pipette tip of claim 1, wherein the pipette adapter further comprises an adapter cup formed by the adapter proximal end and an adapter cup wall extending from the adapter proximal end.

9. 9. The reusable pipette tip of claim 8, wherein the adapter cup wall partially or completely surrounds the adapter proximal end.

10. 10. The reusable pipette tip of claim 8, wherein the adapter cup wall comprises a cup wall guide opposite the adapter proximal end, the cup wall guide comprising a sloped surface.

11. 11. A reusable pipette tip according to any one of claims 8 to 10, wherein the adapter cup wall comprises an inner surface and a radial ring on the inner surface facing towards the center.

12. 12. The reusable pipette tip of claim 11, wherein the adapter cup wall comprises a single radial ring.

13. 13. The reusable pipette tip of claim 11 or 12, wherein the adapter cup wall has a height between 4.01 mm and 3.99 mm.

14. 14. The reusable pipette tip of claim 1, wherein the adapter proximal end comprises a tapered opening surrounding a central lumen.

15. 15. The reusable pipette tip of claim 14, wherein the tapered opening comprises an opening groove and a compliant seal within the opening groove.

16. 16. The reusable pipette tip of claim 1, wherein the adapter proximal end comprises an adapter proximal end groove and a compliant seal within the adapter proximal end groove.

17. (a) a pipettor, a suction block comprising a suction channel and a suction block end, the suction channel having an opening at the suction block end; a pipettor comprising one or more magnets fixed within the aspiration block at an end of the aspiration block; (b) a reusable pipette tip, a reusable pipette tip comprising a pipette adapter having an adapter proximal end, an adapter distal end, adapter sides, and an adapter lumen having openings at the adapter proximal end and the adapter distal end; Equipped with the adapter side portion includes an adapter radial groove; the pipette adapter comprises a magnetically attractable material; the pipette tube comprises a pipette tube proximal end and a pipette tube distal end; the proximal end of the pipette tube is secured within the central lumen opening in the distal end of the adapter; the pipette tip is magnetically attached to an aspiration block face of the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and a channel of the pipette tip; the pipette tip is configured to be retainable by the storage device by receiving and engaging a storage fork of the storage device for holding the pipette tip into the adapter radial groove; Pipetting device.

18. 18. The pipetting device of claim 17, wherein the aspiration block end is substantially flat.

19. 19. The pipetting device of claim 17 or 18, wherein the one or more magnets are slightly recessed from the aspiration block face so that the one or more magnets do not physically contact the pipette adapter.

20. 20. The pipetting device of claim 17, wherein the pipette adapter further comprises an adapter cup formed by the adapter proximal end and an adapter cup wall extending from the adapter proximal end.

21. 21. The pipetting device of claim 20, wherein the adapter cup wall comprises a radial ring configured to contact and align the pipette adapter with the aspiration channel.

22. 22. The pipetting device of claim 20 or 21, wherein the adapter cup wall comprises a cup wall guide opposite the adapter proximal end, the cup wall guide comprising an inclined surface for guiding the aspiration channel into the adapter cup.

23. 20. The pipetting device of any one of claims 17 to 19, wherein the adapter proximal end has a size equal to or smaller than the aspiration block end.

24. 24. The pipetting device of claim 17, wherein the adapter proximal end and the aspiration block face each have an outer periphery, the outer periphery of the adapter proximal end being equal to or smaller than the outer periphery of the aspiration block face.

25. 18. The pipetting device of claim 17, wherein the one or more magnets comprise neodymium iron boron magnets.

26. 18. The pipetting device of claim 17, wherein the one or more magnets comprise four or more magnets arranged in a circle around the suction channel.

27. the suction block end includes an extension through which the suction channel extends; 18. The pipetting device of claim 17, wherein the extension is adapted to fit within an opening in a pipette tip, the opening in the pipette tip being in fluid communication with the aspiration channel.

28. 28. The pipetting device of claim 27, wherein the extension comprises a tapered cone extending from the aspiration block face, the aspiration channel extending through the tapered cone.

29. 18. The pipetting device of claim 17, further comprising a pipette tip sensor in the aspiration block end configured to detect whether a pipette tip is attached to the pipettor.

30. A pipetting device according to any one of claims 17 to 29; a storage device configured to hold one or more of the pipette tips by engaging a storage fork with the adapter radial groove of the pipette tip; 1. A reusable pipette tip system comprising:

31. the storage device comprises a plurality of storage forks, each of the storage forks having storage fork tines separated by a storage fork jaw; 31. The system of claim 30, wherein the storage fork is configured to receive the adapter radial groove into the storage fork mouth in a direction substantially parallel to the storage fork tines and to prevent the inserted adapter from disengaging from the storage fork in a direction substantially perpendicular to the storage fork tines.

32. 32. The system of claim 31, wherein the storage fork mouth is wider at the adapter radial groove than the adapter and narrower than the adapter side adjacent the adapter radial groove.

33. 33. The system of any one of claims 30 to 32, wherein the storage device comprises a storage body to which the storage fork is attached.

34. 34. The system of claim 33, wherein the storage fork is pivotally mounted on a storage block.

35. 35. The system of claim 34, wherein each of the storage forks comprises one or more spring plungers positioned to press against the adapter radial groove of a pipette adapter inserted into the storage fork.

36. 34. The system of claim 33, wherein the storage fork is fixedly mounted on a storage block.

37. 37. The system of any one of claims 31 to 36, wherein the storage fork comprises one or more magnets arranged to magnetically attract the pipette tips.

38. 38. The system of claim 37, wherein the storage device comprises one or more magnets aligned with opposite poles of the one or more magnets in the storage fork.

39. 1. A method of using a reusable pipette tip, comprising: providing a pipetter comprising an aspiration block having an aspiration channel and an aspiration block end, the aspiration channel having an opening at the aspiration block end, and one or more magnets fixed within the aspiration block at the aspiration block end; placing the end of the aspiration block close enough to form a magnetic attachment from a reusable pipette tip; Including, The reusable pipette tip comprises: a pipette adapter comprising a magnetically attractable material, an adapter proximal end, an adapter distal end, an adapter side, and an adapter lumen having openings at the adapter proximal end and the adapter distal end; a pipette tube having a pipette tube proximal end and a pipette tube distal end; Equipped with the adapter side portion includes an adapter radial groove; the proximal end of the pipette tube is secured within the central lumen opening of the distal end of the adapter; the pipette tip is magnetically attached to the pipettor to provide a substantially fluid-tight flow path between the aspiration channel and a channel of the pipette tip; moving the pipettor and the magnetically attached pipette tip to a storage device adapted to receive the pipette adapter; transferring the pipette adapter to the storage device; and holding the pipette tip with the storage device by receiving and engaging a storage fork of the storage device with the adapter radial groove.

40. the storage fork includes storage fork tines separated by a storage fork jaw; the pipettor is moved so that the magnetically attached pipette tip is inserted into the storage fork mouth in a direction substantially parallel to the storage fork blade; 40. The method of claim 39, wherein the pipettor is moved away from the pipette tip engaged with the storage fork in a direction substantially perpendicular to the storage fork tines, thereby overcoming the magnetic attraction between the one or more magnets in the suction body and the pipette adapter while the storage fork tines prevent the inserted adapter from being removed from the storage fork.

41. the storage device further comprises a second storage fork for holding a second pipette tip; moving the pipettor so that the aspiration channel is positioned above the second pipette tip held by the storage fork; moving the pipettor toward the second pipette tip to magnetically attach the pipettor to the second pipette tip; 41. The method of claim 40, further comprising moving the pipettor in a direction substantially parallel to the storage fork tines, thereby removing the second pipette tip from the storage device.

42. 42. The method of claim 41, further comprising detecting whether the second pipette tip is attached to the aspiration channel before removing the second pipette tip from the storage device.

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