Compositions for promoting membrane fusion and uses thereof

Fusosomes, with a lipid bilayer and fusogen, address the challenge of intracellular delivery by enhancing fusion rates and cargo delivery efficiency, offering improved targeting and reduced immunogenicity.

JP7783858B2Active Publication Date: 2025-12-10FLAGSHIP PIONEERING INNOVATIONS V INC
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Patent Information

Application Number
JP2023133407
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Priority Date
2017-12-07
Filing Date
2023-08-18
Publication Date
2025-12-10
Estimated Expiration
2038-05-08

AI Technical Summary

Technical Problem

Delivering large biological agents into cells is hindered by the cell membrane barrier, necessitating new methods for efficient intracellular delivery.

Method used

Fusosomes, comprising a lipid bilayer, a lumen surrounded by the lipid bilayer, and a fusogen, are used to deliver therapeutic agents such as proteins, nucleic acids, and small molecules into cells.

Benefits of technology

Fusosomes enhance the delivery efficiency of biological agents by fusing with target cells at higher rates and delivering cargo effectively, exceeding non-target cells by up to 100-fold, with improved targeting and reduced immunogenicity.

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Abstract

To provide compositions for facilitating membrane fusion and uses thereof.SOLUTION: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can fuse to a target cell, thereby delivering complex biologic agents to the target cell cytoplasm. The present disclosure provides, in some aspects, a fusosome comprising: (a) a lipid bilayer, (b) a lumen (e.g., comprising cytosol) surrounded by the lipid bilayer; (c) an exogenous or overexpressed fusogen, e.g., wherein the fusogen is disposed in the lipid bilayer, the fusosome is derived from a source cell, and the fusosome has partial or complete nuclear inactivation (e.g., nuclear removal).SELECTED DRAWING: Figure 1
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Citation Information

Patent Citations

  • protein delivery system

    JP2008521430A

  • Direct protein delivery using engineered microvesicles

    JP2013510562A

  • Lipid bilayers (protocells) supported on porous nanoparticles for targeted delivery and methods for using the same.

    JP2014512409A

  • Hybridosomes, compositions containing same, methods of their preparation and uses thereof

    JP2017502997A

  • JPP7395355B