Synthesis of vinyl-protected alcohol intermediates

Abstract

Provided herein are methods for synthesizing intermediates useful in the preparation of Mcl-1 inhibitors. In particular, provided herein are methods for synthesizing compound D, which can be prepared by reacting OPG and R 1 Compound D may be useful in the synthesis of compound A1, or a salt or solvate thereof, and compound A2, or a salt or solvate thereof. TIFF2023524263000129.tif98170

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Patent Application No. 63 / 020,862, filed May 6, 2020, which is incorporated by reference in its entirety for all purposes as if fully set forth herein.

[0002] The present disclosure relates to (1S,3'R,6'R,7'S,8'E,11'S,12'R)-6-chloro-7'-methoxy-11',12'-dimethyl-3,4-dihydro-2H,15'H-spiro[naphthalene-1,22'

[20] oxa

[13] thia[1,14]diazatetracyclo[14.7.2.0 3,6 .0 19,24 ]pentacosa[8,16,18,24]tetraen]-15'-one 13',13'-dioxide (Compound A1; AMG176), or a salt or solvate thereof, and (1S,3'R,6'R,7'R,8'E,11'S,12'R)-6-chloro-7'-methoxy-11',12'-dimethyl-7'-((9aR)-octahydro-2H-pyrido[1,2-a]pyrazin-2-ylmethyl)-3,4-dihydro-2H,15'H-spiro[naphthalene-1,22'-

[20] oxa

[13] thia[1,14]diazatetracyclo[14.7.2.0] 3,6 .0 19,24 The present invention relates to a method for synthesizing an intermediate used in the synthesis of pentacosa[8,16,18,24]tetraen]-15'-one 13',13'-dioxide (compound A2; AMG397), or a salt or solvate thereof. These compounds are inhibitors of myeloid cell leukemia 1 protein (Mcl-1). [Background technology]

[0003] The compound (1S,3'R,6'R,7'S,8'E,11'S,12'R)-6-chloro-7'-methoxy-11',12'-dimethyl-3,4-dihydro-2H,15'H-spiro[naphthalene-1,22'

[20] oxa

[13] thia[1,14]diazatetracyclo[14.7.2.0] 3,6 .0 19,24 ]pentacosa[8,16,18,24]tetraen]-15'-one 13',13'-dioxide (compound A1) is useful as an inhibitor of myeloid cell leukemia 1 (Mcl-1), [ka]

[0004] The compound, (1S,3'R,6'R,7'R,8'E,11'S,12'R)-6-chloro-7'-methoxy-11',12'-dimethyl-7'-((9aR)-octahydro-2H-pyrido[1,2-a]pyrazin-2-ylmethyl)-3,4-dihydro-2H,15'H-spiro[naphthalene-1,22'-

[20] oxa

[13] thia[1,14]diazatetracyclo[14.7.2.0] 3,6 .0 19,24 ]Pentacosa[8,16,18,24]tetraen]-15'-one 13',13'-dioxide (Compound A2) is useful as an inhibitor of myeloid cell leukemia 1 (Mcl-1). [ka]

[0005] One common characteristic of human cancers is overexpression of Mcl-1, which prevents cancer cells from undergoing programmed cell death (apoptosis) and allows them to survive despite extensive genetic damage.

[0006] Mcl-1 is a member of the Bcl-2 family of proteins. The Bcl-2 family includes pro-apoptotic members (such as BAX and BAK), which, upon activation, form homo-oligomers in the outer mitochondrial membrane, leading to pore formation and release of mitochondrial contents, a step in the induction of apoptosis. Anti-apoptotic members of the Bcl-2 family (such as Bcl-2, Bcl-XL, and Mcl-1) block the activity of BAX and BAK. Other proteins (such as BID, BIM, BIK, and BAD) exhibit additional regulatory functions. Studies have shown that Mcl-1 inhibitors may be useful in the treatment of cancer. Mcl-1 is overexpressed in many cancers.

[0007] U.S. Patent No. 9,562,061, the entirety of which is incorporated herein by reference, discloses compound A1 as an Mcl-1 inhibitor and provides a method for its preparation. However, improved synthetic methods that result in higher yields and purity of compound A1 are desirable, especially for commercial production of compound A1.

[0008] U.S. Patent No. 10,300,075, the entirety of which is incorporated herein by reference, discloses compound A2 as an Mcl-1 inhibitor and provides a method for its preparation. However, improved synthetic methods that result in higher yields and purity of compound A2 are desirable, particularly for commercial production of compound A2. [Prior art documents] [Patent documents]

[0009] [Patent Document 1] U.S. Patent No. 9,562,061 [Patent Document 2] U.S. Patent No. 10,300,075 Summary of the Invention [Means for solving the problem]

[0010] Provided herein is a method for synthesizing compound D, comprising: [ka] (a) mixing compound B or a salt thereof and compound C in an organic solvent to form a slurry, wherein the organic solvent is selected from the group consisting of a non-polar aromatic solvent, an ether solvent, a chlorinated solvent, an alcohol solvent, acetonitrile, dimethylformamide (DMF), dimethyl carbonate, dimethylacetamide (DMAc), N-methylpyrrolidinone (NMP), and combinations thereof; [ka] (b) combining the slurry of step (a) with a reducing agent to form a mixture comprising compound D, wherein OPG is an alcohol protecting group selected from the group consisting of ether, acetal or ketal, acyl, sulfonyl, and silyl ether; and R 1 is H or C 1~6 alkyl, and R 2 is a protected aldehyde.

[0011] In various embodiments, the method comprises: [ka] in the organic solvent of step (a).

[0012] In various embodiments, compound B is a salt. In various embodiments, R 1 is H. In various embodiments, R 1 is methyl, ethyl, n-propyl, or tert-butyl.

[0013] In various embodiments, the OPG is [ka] (methoxy), [ka] (tert-butyl ether), [ka] (methoxymethyl acetal, MOM), [ka] (2-methoxyethoxymethyl ether, MEM), [ka] (ethoxyethyl acetal, EE), [ka] (methoxypropyl acetal, MOP), [ka] (tetrahydropyranyl acetal, THP), [ka] (benzyloxymethyl acetal, BOM), [ka] (benzyl ether, Bn), [ka] (4-methoxybenzyl ether, PMB), [ka] (2-naphthyl methyl ether, Nap), [ka] (acetyl, Ac), [ka] Pivaloyl (Piv), [ka] (benzoyl, Bz), [ka] (4-bromobenzoyl, Br-Bz), [ka] (4-fluorobenzoyl), [ka] (4-chlorobenzoyl), [ka] (4-iodobenzoyl), [ka] (4-nitrobenzoyl), [ka] (4-phenylbenzoyl), [ka] (1-naphthoyl ester), [ka] (2-naphthoyl ester), [ka] (4-methoxybenzoyl), [ka] (isobutyryl), OSiEt3 (triethylsilyl ether, TES), OSi( iPr)3 (triisopropylsilyl ether, TIPS), OSiMe3 (trimethylsilyl ether, TMS), OSiMe2tBu (tert-butyldimethylsilyl ether, TBS), OSiPh2 t Bu (tert-butyldiphenylsilyl ether, TBDPS), OSO2Me (mesyl), [ka] (4-toluenesulfonyl, tosyl), [ka] (4-nitrobenzenesulfonyl, nosyl), and OSO2CF3 (triflyl). Optionally, OPG is 4-bromobenzoyl.

[0014] In various embodiments, R 2 is a protected aldehyde. In some cases, the protected aldehyde is [ka] is selected from the group consisting of: In some cases, R 2 teeth, [ka] is.

[0015] In various embodiments, compound C has the structure: [ka] It has.

[0016] In various embodiments, compound D has the structure: [ka] It has.

[0017] In various embodiments, the reducing agent is selected from the group consisting of borohydride, borane, silane, Hantzsch ester, formic acid / amine base / metal catalyst, and hydrogen / metal catalyst. In some cases, the reducing agent is NaBH(OAc)3, NaBH3CN, NaBH4, picoline borane (pic-BH3), borane tetrahydrofuran (BH3-THF), decaborane (B 10 H 14 ), borane dimethyl sulfide (BH3-S(Me)2), N-heterocyclic carbene borane (NHC-borane), triethylsilane, phenylsilane, diphenylsilane, [ka] , The reducing agent is selected from the group consisting of H2 / Pd, H2 / Rh, H2 / Ir, H2 / Ru, formic acid / triethylamine / Rh, formic acid / triethylamine / Ir, and formic acid / triethylamine / Ru. In some cases, the reducing agent is NaBH(OAc)3.

[0018] In various embodiments, the organic solvent is selected from the group consisting of toluene, benzene, xylene, tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, diethyl ether, dibutyl ether, diisopropyl ether, dimethoxymethane, 1,2-dimethoxyethane, 1,4-dioxane, dichloromethane (DCM), carbon tetrachloride, chloroform, 1,2-dichloroethane, 2-methyltetrahydrofuran (2-MeTHF), methyl tert-butyl ether (MTBE), and combinations thereof. Optionally, the organic solvent is selected from the group consisting of toluene, THF, DCM, and combinations thereof. Optionally, the organic solvent is toluene.

[0019] In various embodiments, compound B and compound C are present in a molar ratio of B:C of 1:1 to 1:2. In some cases, the molar ratio of B:C is 1:1.1.

[0020] In various embodiments, compound B and the reducing agent are present in a molar ratio of 1:1 to 1:2.25. In some cases, the molar ratio of compound B to reducing agent is 1:1.25.

[0021] In various embodiments, each of steps (a) and (b) is carried out at a temperature of 10° C. to 40° C. In some cases, each of steps (a) and (b) is carried out at a temperature of 20° C.

[0022] In various embodiments, the mixing in step (a) is carried out for 1 minute to 1 hour. In some cases, the mixing in step (a) is carried out for 30 minutes.

[0023] In various embodiments, the reducing agent is added to the slurry within 60 seconds. In various embodiments, the reducing agent is added to the slurry over a period of 10 minutes to 1 hour. In various embodiments, the reducing agent is added to the slurry in two or more equal portions. In some embodiments, the reducing agent is added to the slurry in four equal portions. In some embodiments, the equal portions are added 15 minutes apart.

[0024] In various embodiments, the mixing in step (b) is carried out for 5 to 20 hours. In some cases, the mixing in step (b) is carried out for 16 hours.

[0025] In various embodiments, steps (a) and (b) are carried out sequentially in a single reaction vessel.

[0026] In various embodiments, the method further includes (c) extracting compound D from the mixture of step (b). Optionally, the extracting is via crystallizing compound D. Optionally, the crystallizing is carried out from an organic solvent selected from the group consisting of toluene, benzene, xylene, tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, heptane, diethyl ether, dibutyl ether, diisopropyl ether, dimethoxymethane, dimethoxyethane (DME), 1,4-dioxane, dichloromethane (DCM), carbon tetrachloride, chloroform, 1,2-dichloroethane, 2-methyltetrahydrofuran (2-MeTHF), methyl tert-butyl ether (MTBE), and combinations thereof. Optionally, the crystallizing is carried out from toluene and heptane.

[0027] In various embodiments, the method further includes using compound D to synthesize compound A1, or a salt or solvate thereof. [ka]

[0028] In various embodiments, the method further includes using compound D to synthesize compound A2, or a salt or solvate thereof. [ka]

[0029] Further aspects and advantages will become apparent to those skilled in the art upon review of the following detailed description. The following description herein includes specific embodiments, with the understanding that the disclosure is illustrative and is not intended to limit the invention to the specific embodiments described herein. DETAILED DESCRIPTION OF THE INVENTION

[0030] Provided herein are methods for synthesizing Mcl-1 inhibitors and the corresponding vinyl-protected alcohol intermediates, specifically (1S,3'R,6'R,7'S,8'E,11'S,12'R)-6-chloro-7'-methoxy-11',12'-dimethyl-3,4-dihydro-2H,15'H-spiro[naphthalene-1,22'

[20] oxa

[13] thia[1,14]diazatetracyclo[14.7.2.0] 3,6 .0 19,24 ]pentacosa[8,16,18,24]tetraen]-15'-one 13',13'-dioxide (Compound A1), or a salt or solvate thereof, and a method for synthesizing (1S,3'R,6'R,7'R,8'E,11'S,12'R)-6-chloro-7'-methoxy-11',12'-dimethyl-7'-((9aR)-octahydro-2H-pyrido[1,2-a]pyrazin-2-ylmethyl)-3,4-dihydro-2H,15'H-spiro[naphthalene-1,22'-

[20] oxa

[13] thia[1,14]diazatetracyclo[14.7.2.0] 3,6 .0 19,24 ]pentacosa[8,16,18,24]tetraen]-15'-one 13',13'-dioxide (Compound A2), or a salt or solvate thereof, is provided. [ka]

[0031] U.S. Pat. No. 9,562,061, the entirety of which is incorporated herein by reference, discloses compound A1, or a salt or solvate thereof, as an Mcl-1 inhibitor, and provides a method for preparing the same. The disclosure of salts and solvates of compound A1 from U.S. Pat. No. 9,562,061 is incorporated herein by reference in its entirety. This patent also discloses a method for synthesizing the vinyl alcohol intermediate compound shown below, which is used in the synthesis of compound A1. [ka] Vinyl Alcohol Intermediates of the '061 Patent

[0032] U.S. Patent No. 10,300,075, the entirety of which is incorporated herein by reference, discloses compound A2, or a salt or solvate thereof, as an Mcl-1 inhibitor and provides a method for preparing the same. The disclosure of salts and solvates of compound A2 from U.S. Patent No. 10,300,075 is incorporated by reference in its entirety. This patent also discloses a method for synthesizing the vinyl alcohol intermediate compound shown above, which is used in the synthesis of compound A2.

[0033] The '061 patent outlines a procedure for making a vinyl alcohol intermediate, shown below in Scheme 1, adapted from the disclosure at column 49 of the '061 patent. The '061 patent explains that cyclobutanecarbaldehyde (intermediate II) is combined with oxazepine (intermediate I) in a solvent at a temperature below room temperature, preferably 0° C. Sodium cyanoborohydride is added, and the mixture is added to a sodium hydroxide solution, thereby providing intermediate III. Advantageously, the methods described herein provide an improved synthetic route compared to General Procedure 1 of the '061 patent. The methods described herein can be carried out under ambient conditions (e.g., room temperature) and using milder reagents. Furthermore, the methods can utilize crystalline vinylcyclobutyl intermediates, which allow for improved isolation, storage, and purity throughout the process, compared to the vinylcyclobutyl intermediates of the '061 patent.

[0034] Scheme 1 - General Procedure 1 of the '061 Patent [ka] The '061 patent further describes methods for synthesizing vinyl alcohol intermediates, in which a vinyl group is added to a compound after a fragment containing a cyclobutyl moiety has already been attached to a benzoxazepine moiety. For example, Scheme 2, shown below and adapted from the disclosure in columns 66-71 of the '061 patent, represents the general method for synthesizing vinyl alcohols described in the '061 patent. The '061 patent describes isolating each of the intermediate compounds before use in the next synthetic step. Advantageously, the methods described herein require fewer steps compared to the methods of the '061 patent and do not require isolation of any intermediates. Furthermore, the methods described herein provide convergent fragment assembly of compounds A1 and A2, provide superior purity profiles, improve stability by providing highly crystalline intermediates, and have overall higher yields compared to the '061 patent.

[0035] Scheme 2 - Synthesis of the vinyl alcohol intermediate of the '061 patent [ka] Provided herein is a method for synthesizing compound D, or a salt or solvate thereof, comprising: [ka] As described in detail below, a method is described that includes (a) mixing compound B or a salt thereof with compound C in an organic solvent to form a slurry, and (b) mixing the slurry of step (a) with a reducing agent to form a mixture containing compound D. As will be appreciated, the disclosed method involves forming an intermediate compound formed by the reaction of an amine of compound B with an aldehyde or protected aldehyde of compound C, followed by reducing the intermediate to form the nitrogen-carbon bond of compound D. The methods disclosed herein for forming compound D can be carried out sequentially in a single reaction vessel without the need to isolate the intermediate formed between compounds B and C in step (a). Optionally, compound D can be isolated via extraction (e.g., crystallization) to form crystalline compound D, which can be used in further reactions in the preparation of compound A.

[0036] Process A The disclosed method comprises mixing compound B or a salt thereof with compound C in an organic solvent to form a slurry. It will be understood that the mixture of compounds B and C in step (a) forms an intermediate compound that is not isolated from the coupling of the nitrogen of compound B with the aldehyde or protected aldehyde of compound C.

[0037] As provided herein, Compound B is [ka] (B), or a salt thereof, wherein R 1 is H or C 1-6 In some embodiments, R 1 is H. In other embodiments, R 1 is C 1-6 As used herein, the term "alkyl" refers to a straight or branched chain saturated hydrocarbon group. n The term "C" means that the group has "n" carbon atoms. For example, C alkyl refers to an alkyl group having three carbon atoms. 1~6Alkyl refers to alkyl groups having a number of carbon atoms encompassing all ranges (i.e., 1-6 carbon atoms) as well as all subgroups (e.g., 2-6, 1-5, 1-4, 3-6, 3-5, 1, 2, 3, 4, 5, and 6 carbon atoms). Non-limiting examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl (2-methylpropyl), tert-butyl (1,1-dimethylethyl), n-pentyl, and n-hexyl. In some embodiments, R 1 is methyl, ethyl, n-propyl, or tert-butyl.

[0038] In some embodiments, Compound B is a salt. A salt of Compound B is, for example, in its free base form (i.e., R 1 is H) with a suitable organic or inorganic acid, and optionally isolating the salt thus formed. Non-limiting examples of suitable salts include hydrobromide, hydrochloride, sulfate, bisulfate, sulfonate, camphorsulfonate, phosphate, nitrate, acetate, valerate, oleate, palmitate, stearate, laurate, benzoate, lactate, phosphate, tosylate, citrate, maleate, fumarate, succinate, tartrate, naphthylate, mesylate, glucoheptonate, lactobionate, laurylsulfonate, and amino acid salts. In some embodiments, compound B is a camphorsulfonate salt (i.e., compound B in its free acid form is reacted with camphorsulfonic acid).

[0039] As provided herein, Compound C is [ka] wherein R 2 is CHO (i.e., an aldehyde) or a protected aldehyde, and OPG is an alcohol protecting group. 2 is CHO. In other embodiments, R 2 is a protected aldehyde.

[0040] The term "protected aldehyde" or "aldehyde protecting group" refers to any protecting group used to mask an aldehyde functionality. Aldehyde protecting groups include acetals and hemiacetals. Acetals and hemiacetals are C 1-8 Alcohol or C 2-8 The protected aldehyde can be prepared from a diol. In some embodiments, the protected aldehyde is a 5- or 6-membered cyclic acetal formed from the condensation of an aldehyde with ethylene or propylene glycol. In some embodiments, the protected aldehyde is an imine or hydroxyimine. In some embodiments, the protected aldehyde comprises a bisulfite or a benzotriazole. In some embodiments, the protected aldehyde is [ka] In some embodiments, the protected aldehyde is selected from the group consisting of: [ka] In some embodiments, the protected aldehyde is [ka] where the counter ion is, for example, a sodium ion.

[0041] Alcohol protecting groups are groups that mask hydroxyl functional groups and are well known in the art. Preparation of compounds can involve protecting and deprotecting various hydroxyl groups. The need for protection and deprotection, and the selection of appropriate protecting groups, can be easily determined by those skilled in the art. The chemical properties of protecting groups can be found, for example, in Greene, et al., Protective Groups in Organic Synthesis, 4th Ed., Wiley & Sons, 2007, which is incorporated herein by reference in its entirety. Adjustments to the alcohol protecting groups and formation and cleavage methods described herein can be made as needed to take into account various substituents. In some cases, the alcohol protecting group, OPG, is selected from the group consisting of ether, acetal or ketal, acyl, sulfonyl, and silyl ether.

[0042] In some embodiments, OPG is an ether. Ether protecting groups include either a substituted or unsubstituted alkyl moiety attached to the oxygen from the hydroxyl group to be protected (e.g., masked as an ether). Examples of suitable ethers include, but are not limited to, methoxy, ethoxy, propoxy, butoxy, methoxymethyl acetal (MOM), 2-methoxyethoxyethoxyethyl ester (MEM), ethoxyethyl acetal (EE), and methoxypropyl ether (MOP). Other examples of contemplated ethers include, but are not limited to, benzyloxymethyl acetal (BOM), benzyl ether (Bn), 4-methoxybenzyl ether (PMB), and 2-naphthylmethyl ether (Nap).

[0043] In some embodiments, OPG is an acetal or ketal. Acetals as protecting groups include: [ka] which may be an acetal (OR' as an option, where R' is, for example, an alkyl group) or a hemiacetal (OH as an option), where RO is derived from a protected hydroxyl group and PG' is the remainder of a (hemi)acetal protecting group. Ketals as protecting groups are [ka] where RO is derived from a protected hydroxyl group and may be a ketal (OR' as an option, where R' is, for example, an alkyl group) or a hemiketal (OH as an option), and each PG' is derived from the remainder of a (hemi)ketal protecting group masking a hydroxyl group (i.e., R-OH) and may be substituted or unsubstituted. Examples of suitable acetals include, but are not limited to, tetrahydropyranyl acetal (THP).

[0044] In some embodiments, the OPG is acyl. As used herein, the term "acyl" refers to an alcohol protecting group in which the oxygen atom of the alcohol is attached to the acyl group. [ka] where RO is derived from a protected hydroxyl group and PG' is derived from the remainder of an acyl protecting group. Examples of suitable acyl include, but are not limited to, acetyl (Ac), pivaloyl (Piv), benzoyl (Bz), 4-bromobenzoyl (Br-Bz), 4-fluorobenzoyl, 4-chlorobenzoyl, 4-iodobenzoyl, 4-nitrobenzoyl, 4-phenylbenzoyl, 1-naphthoyl ester, 2-naphthoyl ester, 4-methoxybenzoyl, and isobutyryl.

[0045] In some embodiments, OPG is a silyl ether. As used herein, the term "silyl ether" refers to an alcohol protecting group in which the oxygen atom of the alcohol is bonded to a silyl ether group. [ka] where RO is derived from a protected hydroxyl group and each PG' is derived from the remainder of a silyl ether protecting group. Examples of suitable silyl ethers include, but are not limited to, triethylsilyl ether (TES), triisopropylsilyl ether (TIPS), trimethylsilyl ether (TMS), tert-butyldimethylsilyl ether (TBS), and tert-butyldiphenylsilyl ether (TBDPS).

[0046] In some embodiments, OPG is a sulfonyl protecting group. As used herein, the term "sulfonyl protecting group" refers to an alcohol protecting group in which the oxygen atom of the alcohol is bonded to a sulfonyl group; [ka] wherein RO is derived from a protected hydroxyl group and PG' is derived from the remainder of a sulfonyl protecting group, in some embodiments, the sulfonyl protecting group is selected from the group consisting of mesyl, tosyl, nosyl, and triflyl.

[0047] In some embodiments, OPG is [ka] (methoxy), [ka] (tert-butyl ether), [ka] (methoxymethyl acetal, MOM), [ka] (2-methoxyethoxymethyl ether, MEM), [ka] (ethoxyethyl acetal, EE), [ka] (methoxypropyl acetal, MOP), [ka] (tetrahydropyranyl acetal, THP), [ka] (benzyloxymethyl acetal, BOM), [ka] (benzyl ether, Bn), [ka] (4-methoxybenzyl ether, PMB), [ka] (2-naphthyl methyl ether, Nap), [ka] (acetyl, Ac), [ka] Pivaloyl (Piv), [ka] (benzoyl, Bz), [ka] (4-bromobenzoyl, Br-Bz), [ka] (4-fluorobenzoyl), [ka] (4-chlorobenzoyl), [ka] (4-iodobenzoyl), [ka] (4-nitrobenzoyl), [ka] (4-phenylbenzoyl), [ka] (1-naphthoyl ester), [ka] (2-naphthoyl ester), [ka] (4-methoxybenzoyl), [ka] (isobutyryl), OSiEt3 (triethylsilyl ether, TES), OSi( i Pr)3 (triisopropylsilyl ether, TIPS), OSiMe3 (trimethylsilyl ether, TMS), OSiMe2tBu (tert-butyldimethylsilyl ether, TBS), OSiPh2 t Bu (tert-butyldiphenylsilyl ether, TBDPS), OSO2Me (mesyl), [ka] (4-toluenesulfonyl, tosyl), [ka] (4-nitrobenzenesulfonyl, nosyl), and OSO2CF3 (triflyl). In some embodiments, OPG is 4-bromobenzoyl.

[0048] In some embodiments, OPG is 4-bromobenzoyl and R 1 teeth, [ka] That is, compound C is [ka] It has the following structure.

[0049] The mixing of compounds B and C in step (a) is carried out in the presence of an organic solvent. Compound B and compound C may be present in the organic solvent in a B:C molar ratio of 1:1 to 1:2, e.g., at least 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, or 1:1.5, and / or at most 1:2, 1:1.9, 1:1.7, 1:1.6, 1:1.5, or 1:1.4, e.g., 1:1 to 1:1.5, 1:1 to 1:1.4, or 1:1.1 to 1:1.3. In some embodiments, the molar ratio of compound B to compound C is 1:1.1.

[0050] The organic solvent in step (a) is selected from the group consisting of nonpolar aromatic solvents, ether solvents, chlorinated solvents, alcohol solvents, acetonitrile, dimethylformamide (DMF), dimethylacetamide (DMAc), N-methylpyrrolidinone (NMP), dimethyl carbonate, and combinations thereof. Non-limiting examples of nonpolar aromatic solvents include toluene, benzene, xylene, chlorobenzene, fluorobenzene, naphthalene, and benzotrifluoride. Non-limiting examples of ether solvents include tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, diethyl ether, dibutyl ether, diisopropyl ether, methyl tert-butyl ether (MTBE), 1,2-dimethoxyethane, 1,4-dioxane, 2-methyltetrahydrofuran (2-MeTHF), and cyclopentyl methyl ether. Non-limiting examples of chlorinated solvents include 1,2-dichloroethane, chloroform, carbon tetrachloride, and dichloromethane. Non-limiting examples of alcohol solvents include methanol, ethanol, propanol, 2-propanol, and tert-butanol.

[0051] In some embodiments, the organic solvent is selected from the group consisting of toluene, benzene, xylene, tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, diethyl ether, dibutyl ether, diisopropyl ether, dimethoxymethane, 1,2-dimethoxyethane, 1,4-dioxane, dichloromethane (DCM), carbon tetrachloride, chloroform, 1,2-dichloroethane, 2-methyltetrahydrofuran (2-MeTHF), methyl tert-butyl ether (MTBE), and combinations thereof. In some embodiments, the organic solvent is selected from the group consisting of toluene, THF, DCM, and combinations thereof. In some embodiments, the organic solvent is toluene.

[0052] Solvent can be included in an amount of 5 L / kg Compound B to 25 L / kg Compound B, e.g., at least about 5, 10, 15, or 20 L / kg Compound B and / or up to about 25, 20, 25, or 10 L / kg Compound B, e.g., 5 L / kg to 20 L / kg, 5 L / kg to 15 L / kg, or 5 L / kg to 10 L / kg Compound B. In some embodiments, solvent is present in an amount of 10 L / kg Compound B.

[0053] In some embodiments, step (a) comprises: [ka] (camphorsulfonic acid) with Compounds B and C in an organic solvent. As described herein, camphorsulfonic acid can be used to prepare a salt form of Compound B. Advantageously, the salt form of Compound B can be formed in situ and does not need to be isolated prior to use in step (a) of the methods described herein.

[0054] Step (a) can be carried out at a temperature of 10° C. to 40° C., e.g., at least 10, 12, 15, 17, 20, 22, 25, 27, or 30° C., and / or at most 40, 37, 35, 32, 30, 27, 25, 22, or 20° C., e.g., 10° C. to 30° C., 12° C. to 27° C., 15° C. to 25° C., or 17° C. to 22° C. In some embodiments, step (a) is carried out at a temperature of 20° C.

[0055] The mixing in step (a) can be carried out for a period of time ranging from 1 minute to 1 hour, e.g., at least 1, 5, 10, 15, 20, 25, 30, 35, or 40 minutes, and / or up to 60, 55, 50, 45, 40, 35, 30, 25, or 20 minutes, e.g., 5 to 45 minutes, 10 to 40 minutes, 15 to 40 minutes, 20 to 45 minutes, or 25 to 35 minutes. In some embodiments, the mixing in step (a) is carried out for 30 minutes.

[0056] Process B The disclosed method includes combining the slurry of step (a) with a reducing agent to form a mixture comprising compound D. Step (b) may be carried out in the same or a different reaction vessel as step (a). In some embodiments, step (b) is carried out in the same reaction vessel as step (a).

[0057] As provided herein, compound D is [ka] where R 1 and OPG are each as described herein. In some embodiments, R 1 is H and OPG is 4-bromobenzoyl, i.e., compound D has the structure [ka] It has.

[0058] The reducing agent is any agent that reduces the nitrogen-carbon double bond formed between compounds B and C in step (a). The reducing agent can be, for example, a metal hydride, a hydrogen / metal catalyst, an organometallic reagent, or an electron donor used in combination with a proton, where the electron is donated by a cathode or a metal selected from Li, Na, K, Mg, Zn, Fe, and Al. Metal hydrides include boron hydrides and aluminum hydrides. Examples of boron or aluminum hydrides include NaBH, NaB(CN)H, NaBH(OAc), LiAlH, LiAlH(Otert-Bu), LiBH, LiBHEt, and LiBH(sec-Bu). In some cases, the reducing agent is selected from the group consisting of borohydrides, boranes, silanes, Hantzsch esters, formic acid / amine base / metal catalysts, and hydrogen / metal catalysts. Hantzsch's ester, 1,4-dihydrobenzol, isopropanol, formic acid, and ammonium formate are transfer hydrogenation reagents and, because they can transfer hydride ions and protons, are hydrogen sources. Reactions with these transfer hydrogenation reagents can be carried out metal-free, i.e., in the absence of a metal catalyst.

[0059] In some embodiments, the reducing agent is borohydride. Examples of suitable borohydrides include, but are not limited to, NaBH(OAc)3, NaBH3CN, and NaBH4. In some embodiments, the reducing agent is NaBH(OAc)3.

[0060] In some embodiments, the reducing agent is a borane. Examples of suitable boranes include picoline borane (pic-BH), borane tetrahydrofuran (BH-THF), decaborane (B 10 H 14 ), borane dimethyl sulfide (BH3-S(Me)2), and N-heterocyclic carbene boranes (NHC-boranes). One example of an NHC-borane is 1,3-dimethylimidazol-2-ylidene borane (diMe-Imd-BH3).

[0061] In some embodiments, the reducing agent is a silane. Examples of suitable silanes include, but are not limited to, triethylsilane, phenylsilane, diphenylsilane, trichlorosilane, triphenylsilane, (tris)trimethylsilane, and phenylmethylsilane.

[0062] In some embodiments, the reducing agent is a Hantzsch ester. Examples of suitable Hantzsch esters include: [ka] These include, but are not limited to:

[0063] In some embodiments, the reducing agent is formic acid / amine base / metal catalyst. Examples of suitable amine bases include tertiary amine bases such as triethylamine and diisopropylethylamine. Examples of suitable metal catalysts include, but are not limited to, palladium (Pd), iridium (Ir), ruthenium (Ru), and rhodium (Rh). Examples of suitable formic acid / amine base / metal catalysts include, but are not limited to, formic acid / triethylamine / Ir, formic acid / triethylamine / Ru, and formic acid / triethylamine / Rh.

[0064] In some embodiments, the reducing agent is a hydrogen / metal catalyst, including, but not limited to, H2 / Pd, H2 / Rh, H2 / Ir, and H2 / Ru.

[0065] In some embodiments, the reducing agent is NaBH(OAc)3, NaBH3CN, NaBH4, picoline borane (pic-BH3), borane tetrahydrofuran (BH3-THF), decaborane (B 10 H 14 ), borane dimethyl sulfide (BH3-S(Me)2), N-heterocyclic carbene borane (NHC-borane), triethylsilane, phenylsilane, diphenylsilane, [ka] , It is selected from the group consisting of H2 / Pd, H2 / Rh, and H2 / Ru, formic acid / triethylamine / Rh, formic acid / triethylamine / Ir, and formic acid / triethylamine / Ru.

[0066] Compound B and the reducing agent can be present in a molar ratio of 1:1 to 1:2.25, e.g., at least 1:1, 1:1.25, 1:1.5, 1:1.75, or 1:2, and / or at most 1:2.25, 1:1.2, 1:1.75, 1:1.5, or 1:1.25, e.g., 1:1 to 1:2, 1:1 to 1:1.75, or 1:1 to 1:1.5. In some embodiments, the molar ratio of compound B to reducing agent is 1:1.25.

[0067] The reducing agent can be added to the slurry in step (a) in a single addition (i.e., all at once) or gradually (i.e., in small increments) over time. It will be appreciated that actually adding the reducing agent to the slurry "all at once" may take, for example, more than one second when dealing with large-scale reactions (e.g., kilogram-scale materials). Thus, in some embodiments, the reducing agent is added to the slurry in 60 seconds or less, or even 30 seconds or less, or 10 seconds or less, and is considered to have been added to the slurry all at once. If the reducing agent is not added all at once, the reducing agent may be added in small increments or continuously over a longer period of time. In some embodiments, the reducing agent is added to the slurry over a period of 10 minutes to 1 hour, e.g., at least 10, 15, 20, 25, 30, 35, or 40 minutes, and / or up to 60, 55, 50, 45, 40, 35, 30, or 25 minutes, e.g., 10-45 minutes, 15-30 minutes, or 20-30 minutes. In some embodiments, the reducing agent is added to the slurry in two or more equal portions. In some embodiments, the reducing agent is added to the slurry in four equal portions. In some embodiments, two or more (e.g., four) equal portions are added 15 minutes apart.

[0068] Step (b) can be carried out at a temperature of 10°C to 40°C, e.g., at least 10, 12, 15, 17, 20, 22, 25, 27, or 30°C, and / or at most 40, 37, 35, 32, 30, 27, 25, 22, or 20°C, e.g., 12°C to 35°C, 15°C to 30°C, 17°C to 25°C, or 20°C to 25°C. In some embodiments, step (b) is carried out at a temperature of 20°C.

[0069] The mixing in step (b) can be carried out for 5 to 20 hours, e.g., at least 5, 7, 10, 12, 16, or 15 hours, and / or at most 20, 17, 16, 15, 12, 10, or 7 hours, e.g., 10 to 20 hours, 12 to 20 hours, 12 to 17 hours, or 15 to 17 hours. In some embodiments, the mixing is carried out for 16 hours.

[0070] Process C In some embodiments, the disclosed method may further include extracting compound D from the mixture of step (b). In some embodiments, the extracting is carried out by crystallizing compound D from the mixture. The crystallization may be carried out in an organic solvent selected from the group consisting of toluene, benzene, xylene, tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, heptane, diethyl ether, dibutyl ether, diisopropyl ether, dimethoxymethane, dimethoxyethane (DME), 1,4-dioxane, dichloromethane (DCM), carbon tetrachloride, chloroform, 1,2-dichloroethane, 2-methyltetrahydrofuran (2-MeTHF), methyl tert-butyl ether (MTBE), and combinations thereof. In some embodiments, compound D is crystallized from the mixture using toluene and heptane. Optionally, seed crystals of compound D are added during crystallization to aid in the formation of compound D crystals. Optionally, the compound D crystals are filtered and dried.

[0071] The method for synthesizing compound D can be used to synthesize compounds A1 and A2. As shown in Scheme 3 below, compound D can be used to synthesize compound A1, or a salt or solvate thereof. As shown in Scheme 4 below, compound D can be used to synthesize compound A2, or a salt or solvate thereof.

[0072] Scheme 3 - Conversion of Compound D to Compound A1 [ka] Compound D can be used to synthesize compound A1 and its salts and solvates, as shown in Scheme 3 and described in U.S. Pat. No. 9,562,061. The synthesis of sulfonamide EE22 is disclosed in U.S. Pat. No. 9,562,061. Compounds EE22 and D can be reacted to form compound E. As described in U.S. Pat. No. 9,562,061, cyclization and deprotection of compound E provides compound F, which can then be methylated to provide compound A1.

[0073] Scheme 4 - Conversion of Compound D to Compound A2 [ka] Compound D can be used to synthesize compound A2 and its salts and solvates, as shown in Scheme 4 and described in U.S. Pat. No. 10,300,075. As described above with respect to Scheme 3, the synthesis of sulfonamide EE22 is disclosed in U.S. Pat. No. 9,562,061. Compounds EE22 and D (free acid) react to form the compound G which can be cyclized to form the compound H As disclosed in U.S. Pat. No. 10,300,075, the compound H is subsequently oxidized to give the compound I Alternatively, the compound G is oxidized to form the compound I can be used to obtain the uncyclized version of I Using the procedure disclosed in U.S. Pat. No. 10,300,075, the compound I is subsequently epoxidized to give the compound J The compound J followed by bicyclic compounds K to form the compound L Finally, the compound L Methylation of the compound A2 gives compound A2, which is disclosed in US Pat. No. 10,300,075.

[0074] In some embodiments, the method further comprises using compound D to synthesize compound A1, or a salt or solvate thereof. [ka]

[0075] In some embodiments, the method further comprises using compound D to synthesize compound A2, or a salt or solvate thereof. [ka]

[0076] While this disclosure is to be read in conjunction with its detailed description, it is to be understood that the foregoing description and the following examples are exemplary and do not limit the scope of the disclosure, which is intended to be defined by the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims. [Example]

[0077] The following examples are provided for illustrative purposes and are not intended to limit the scope of the present invention.

[0078] Example 1 [ka] A 500 mL glass-lined, jacketed reactor was charged with 25.0 g of compound B (1.0 equiv., 43.4 mmol), followed by 21.9 g of compound C (1.1 equiv., 47.7 mmol, 70.4 wt %) and 250 mL of toluene (10 L / kg). The resulting slurry mixture was stirred at 20 °C for 30 minutes. Subsequently, NaBH(OAc)3 (11.5 g, 1.25 equiv.) was charged to the reactor at 20 °C in 0.25 equiv. portions, with each portion added at least 15 minutes apart. The reaction was stirred at 20 °C for ≥ 5 hours until LC analysis confirmed complete consumption of compound B. Aqueous solutions of NaCl and NaHCO3 were slowly charged to the reaction mixture to suppress the observed gas evolution. The batch was stirred at 20 °C for > 30 minutes. After phase separation, the aqueous phase was removed. Aqueous H3PO4 was charged to the reactor containing the organic phase, and the resulting mixture was stirred at 20°C for >15 minutes. After phase separation, the aqueous phase was removed. This aqueous H3PO4 washing procedure was repeated two more times. Aqueous NaCl was charged to the reactor containing the organic phase, and the mixture was stirred at 20°C for >15 minutes. After phase separation, the aqueous phase was removed. The batch was then concentrated under reduced pressure at ≤55°C, and the batch was then cooled to 20°C. Compound D seeds were charged to the reactor to induce crystallization, and the slurry was held at 20°C for >1 hour. Heptane was then charged to the reactor. After addition, the suspension was stirred at 20°C for >1 hour. After filtration and washing with 2 / 1 heptane / toluene, compound D was obtained, which was dried under vacuum at 40°C. These two steps yielded compound D in an isolated yield of 85.5% by weight and 85.0%.

[0079] Compound D: 11H NMR (600 MHz, CDCl3) δ 7.86 (d, J = 8.6 Hz, 2H), 7.64 (d, J = 8.5 Hz, 1H), 7.50 (d, J = 8.6 Hz, 2H), 7.47 (dd, J = 8.2, 1.9 Hz, 1H), 7.44 (d, J = 1.9 Hz, 1H), 7.16 (dd, J = 8.5, 2.3 Hz, 1H), 7.08 (d, J = 2.3 Hz, 1H), 6.93 (d, J = 8.2 Hz, 1H), 5.84 (ddd, J = 17.1, 10.6, 6.4 Hz, 1H), 5.49 (bt, J = 6.4 Hz, 1H), 5.36 (dt, J = 17.1, 1.2 Hz, 1H), 5.22 (dt, J = 10.6, 1.2 Hz, 1H), 4.12 (d, J = 12.1 Hz, 1H), 4.08 (d, J = 12.1 Hz, 1H), 3.59 (dd, J = 14.8, 4.1 Hz, 1H), 3.52 (d, J = 14.4 Hz, 1H), 3.35 (dd, J = 14.8, 9.0 Hz, 2H), 3.32 (d, J = 14.4 Hz, 1H), 2.78 - 2.75 (m, 1H), 2.75 - 2.71 (m, 2H), 2.47 (qui, J = 8.5 Hz, 1H), 2.12 - 2.02 (m, 1H), 2.00 - 1.92 (m, 1H), 1.93 - 1.85 (m, 2H), 1.85 - 1.77 (m, 1H), 1.78 - 1.69 (m, 2H), 1.56 (bt, J = 11.0 Hz, 1H); 13 13C NMR (151 MHz, CDCl3) δ 171.8, 165.1, 153.7, 141.0, 139.0, 138.8, 134.3, 132.1, 131.7, 131.0, 129.5, 129.1, 128.6, 128.1, 126.6, <123.7>, <121.7>, <120.8>, <117.5>, <117.0>, <79.4>, <78.0>, <60.9>, <58.8>, <43.0>, <41.8>, <36>.2, <30.2>, <29.0>, <25.9>, <21.2>, <19.0>. LRMS (ESI): Calculated: 650; Observed: 650.1.

Claims

1. A method for synthesizing compound (D), comprising: 【Chemistry 1】 (a) mixing compound (B) or a salt thereof and compound (C) in an organic solvent to form a slurry, wherein the organic solvent is selected from the group consisting of non-polar aromatic solvents, ether solvents, chlorinated solvents, alcohol solvents, acetonitrile, dimethylformamide (DMF), dimethyl carbonate, dimethylacetamide (DMAc), N-methylpyrrolidinone (NMP), and combinations thereof; 【Chemistry 2】 and (b) mixing the slurry of step (a) with a reducing agent to form a mixture comprising compound (D); where: OPG is an alcohol protecting group selected from the group consisting of ether, acetal or ketal, acyl, sulfonyl, and silyl ether; R １ is H or C １～６ is alkyl, R ２ is a protected aldehyde, and the protected aldehyde is a group 【Transformation 3】 The method is selected from the group consisting of:

2. A compound of the formula: 【Chemistry 4】 in the organic solvent in step (a).

3. The method of claim 2, wherein compound (B) is a salt.

4. R １ The method of claim 1 , wherein

5. R １ The method of claim 1, wherein is methyl, ethyl, n-propyl, or tert-butyl.

6. OPG is a group of the formula: 【Transformation 5】 (methoxy), Based on the formula: 【Transformation 6】 (tert-butyl ether), Based on the formula: 【Transformation 7】 (methoxymethyl acetal, MOM), Based on the formula: 【Transformation 8】 (2-methoxyethoxymethyl ether, MEM), Based on the formula: 【Chemistry 9】 (ethoxyethyl acetal, EE), Based on the formula: 【Chemistry 10】 (methoxypropyl acetal, MOP), Based on the formula: 【Chemistry 11】 (tetrahydropyranyl acetal, THP), Based on the formula: 【Chemistry 12】 (benzyloxymethyl acetal, BOM), Based on the formula: 【Chemistry 13】 (benzyl ether, Bn), Based on the formula: 【Chemistry 14】 (4-methoxybenzyl ether, PMB), Based on the formula: 【Chemistry 15】 (2-naphthyl methyl ether, Nap), Based on the formula: 【Chemistry 16】 (acetyl, Ac), Based on the formula: 【Chemistry 17】 pivaloyl (Piv), Based on the formula: [Chemistry 18] (benzoyl, Bz), Based on the formula: 【Chemistry 19】 (4-bromobenzoyl, Br-Bz), Based on the formula: 【Chemistry 20】 (4-fluorobenzoyl), Based on the formula: 【Chemistry 21】 (4-chlorobenzoyl), Based on the formula: 【Chemistry 22】 (4-iodobenzoyl), Based on the formula: 【Chemistry 23】 (4-nitrobenzoyl), Based on the formula: 【Chemistry 24】 (4-phenylbenzoyl), Based on the formula: 【Chemistry 25】 (1-naphthoyl ester), Based on the formula: 【Chemistry 26】 (2-naphthoyl ester), Based on the formula: 【Chemistry 27】 (4-methoxybenzoyl), Based on the formula: 【Chemistry 28】 (isobutyryl), OSiEt ３ (triethylsilyl ether, TES), OSi ( ｉ Pr) ３ (triisopropylsilyl ether, TIPS), OSiMe ３ (trimethylsilyl ether, TMS), OSiMe ２ tBu (tert-butyldimethylsilyl ether, TBS), OSiPh ２ ｔ Bu (tert-butyldiphenylsilyl ether, TBDPS), OSO ２ Me (Mesir), Based on the formula: 【Chemistry 29】 (Tosir), Based on the formula: 【Transformation 30】 (Nosyl), and OSO ２ CF ３ 2. The method of claim 1, wherein the alkyl group is selected from the group consisting of (triflyl).

7. 7. The method of claim 6, wherein OPG is 4-bromobenzoyl.

8. R ２ is a group of the following formula: 【Chemistry 31】 The method of claim 1, wherein

9. Compound (C) has the following structure: 【Chemistry 32】 The method according to claim 8, wherein the compound is represented by the formula:

10. Compound (D) has the following structure: 【Transformation 33】 The method according to claim 1, wherein the compound is represented by the formula:

11. 10. The method of claim 1, wherein the reducing agent is selected from the group consisting of borohydrides, boranes, silanes, Hantzsch esters, formic acid / amine base / metal catalysts, and hydrogen / metal catalysts.

12. The reducing agent is NaBH(OAc) ３ , NaBH ３ CN, NaBH ４ , picoline borane (pic-BH ３ ), borane tetrahydrofuran (BH ３ -THF), decaborane (B １０ H １４ ), borane dimethyl sulfide (BH ３ -S(Me) ２ ), N-heterocyclic carbene borane (NHC-borane), triethylsilane, phenylsilane, diphenylsilane, A compound of the formula: 【Transformation 34】 , a compound of the formula: 【Chemistry 35】 , a compound of the formula: 【Transformation 36】 , a compound of the formula: 【Chemistry 37】 , H ２ / Pd, H ２ / Rh, H ２ / Ir, H ２ 12. The method of claim 11 , wherein the base is selected from the group consisting of formic acid / triethylamine / Ru, formic acid / triethylamine / Rh, formic acid / triethylamine / Ir, and formic acid / triethylamine / Ru.

13. The reducing agent is NaBH(OAc) ３ The method of claim 12, wherein

14. 2. The method of claim 1, wherein the organic solvent is selected from the group consisting of toluene, benzene, xylene, tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, diethyl ether, dibutyl ether, diisopropyl ether, dimethoxymethane, 1,2-dimethoxyethane, 1,4-dioxane, dichloromethane (DCM), carbon tetrachloride, chloroform, 1,2-dichloroethane, 2-methyltetrahydrofuran (2-MeTHF), methyl tert-butyl ether (MTBE), and combinations thereof.

15. 15. The method of claim 14, wherein the organic solvent is selected from the group consisting of toluene, THF, DCM, and combinations thereof.

16. 16. The method of claim 15, wherein the organic solvent is toluene.

17. 10. The method of claim 1, wherein compound (B) and compound (C) are present in a molar ratio of (B):(C) of 1:1 to 1:

2.

18. 18. The method of claim 17, wherein the molar ratio of (B):(C) is 1:1.

1.

19. 2. The method of claim 1, wherein compound (B) and the reducing agent are present in a molar ratio of 1:1 to 1:2.

25.

20. 20. The method of claim 19, wherein the molar ratio of compound (B) to the reducing agent is 1:1.

25.

21. 10. The method of claim 1, wherein each of steps (a) and (b) is carried out at a temperature of from 10°C to 40°C.

22. 22. The method of claim 21, wherein each of steps (a) and (b) is carried out at a temperature of 20°C.

23. 10. The method of claim 1, wherein the mixing in step (a) is carried out for 1 minute to 1 hour.

24. 24. The method of claim 23, wherein the mixing in step (a) is carried out for 30 minutes.

25. 10. The method of claim 1, wherein the reducing agent is added to the slurry within 60 seconds.

26. 10. The method of claim 1, wherein the reducing agent is added to the slurry over a period of 10 minutes to 1 hour.

27. 27. The method of claim 26, wherein the reducing agent is added to the slurry in two or more equal portions.

28. 28. The method of claim 27, wherein the reducing agent is added to the slurry in four equal portions.

29. 28. The method of claim 27, wherein the aliquots are added at 15 minute intervals.

30. 10. The method of claim 1, wherein the mixing in step (b) is carried out for 5 hours to 20 hours.

31. 31. The method of claim 30, wherein the mixing in step (b) is carried out for 16 hours.

32. 10. The method of claim 1, wherein steps (a) and (b) are carried out sequentially in a single reaction vessel.

33. 10. The method of claim 1, further comprising: (c) extracting compound (D) from the mixture of step (b).

34. 34. The method of claim 33, wherein the extraction is via crystallization of compound (D).

35. 35. The method of claim 34, wherein the crystallization is carried out in an organic solvent selected from the group consisting of toluene, benzene, xylene, tetrahydrofuran (THF), tetrahydropyran, tetrahydrofurfuryl alcohol, heptane, diethyl ether, dibutyl ether, diisopropyl ether, dimethoxymethane, dimethoxyethane (DME), 1,4-dioxane, dichloromethane (DCM), carbon tetrachloride, chloroform, 1,2-dichloroethane, methyl tert-butyl ether (MTBE), 2-methyltetrahydrofuran (2-MeTHF), and combinations thereof.

36. 36. The method of claim 35, wherein the crystallization is carried out in toluene and heptane.

37. Compound (A1): 【Transformation 38】 or a salt or solvate thereof, comprising the method of claim 1, Further, the following steps: a) reacting the compound (D) obtained by the method according to claim 1 with the compound (EE22): 【Chemistry 39】 to obtain compound (E): 【Chemistry 40】 forming a b) The obtained compound (E) is cyclized, and the protecting group (PG) is removed to give compound (F): 【Chemistry 41】 providing c) methylating the obtained compound (F) to synthesize the compound (A1) or a salt or solvate thereof.

38. Compound (A2): 【Chemistry 42】 or a salt or solvate thereof, comprising the method of claim 1, Further, the following steps: a') Removing the protecting group (PG) and, when R 1 is C 1-6 alkyl, R 1 from the compound (D) obtained by the method of claim 1 to obtain a deprotected carboxylic acid form of the compound (D) having the following structure: 【Chemistry 43】 obtaining a compound represented by the formula: b') Compound (EE22): 【Chemistry 44】 with the deprotected carboxylic acid form of compound (D) obtained in step a′) to obtain compound (G): 【Chemistry 45】 forming a c') The obtained compound (G) is cyclized to obtain a compound (H): 【Chemistry 46】 providing d') The resulting compound (H) is oxidized to give a ketone-containing compound (I): 【Chemistry 47】 forming a e') epoxidizing the obtained compound (I) to obtain an epoxidized compound (J): 【Chemistry 48】 preparing f') The obtained compound (J) is converted into a bicyclic compound (K): 【Chemistry 49】 to obtain compound (L): [Transformation 50] and g') methylating the obtained compound (L) to synthesize the compound (A2) or a salt or solvate thereof.

Images