Pipetting element

Abstract

The present application relates to laboratory devices, and in particular to a pipetting element. The pipetting element comprises a body and an attraction member arranged in the body; the body is conical, and the body sequentially comprises a connecting section, a liquid storage section and a liquid suction section from the thick end to the fine end of the cone; the connecting section is adapted to a pipette; the attraction member is a magnetic attraction member. The pipetting element can simply and quickly separate magnetic beads and a liquid, has low cost, is quick and simple to operate, and can improve the experiment efficiency, reduce the machine manufacturing cost, and reduce the labor cost.

Description

A pipetting element Technical Field

[0001] The present application relates to an experimental instrument, and in particular to a pipetting element. Background Art

[0002] A pipette is a common tool for transferring liquids, used in conjunction with a consumable pipette tip. During use, the pipette is fitted onto the pipette tip, and the pipette is operated to expel some of the air from the tip. Atmospheric pressure is then used to cause the tip to absorb the liquid from the container. After reaching the destination, the pipette is operated again to push the air out of the tip, releasing the liquid absorbed by the tip, thereby completing the transfer. Existing pipette tips generally have a simple conical shell structure. More complex ones have a filter element inside the conical shell to prevent damage to the pipette and sample when transferring substances that may contaminate the pipette (e.g., bacteria, viruses, volatile, corrosive, or viscous chemicals). However, the two existing pipette tips can only transfer liquids and cannot be used for more complex operations.

[0003] In the prior art, to separate magnetic beads from the liquid containing the beads, the process involves placing a centrifuge tube containing the liquid containing the beads on a magnetic rack. After a period of magnetic attraction, the beads are completely absorbed by the rack. The supernatant is then carefully aspirated and discarded using a pipette. This process is repeated several times to achieve separation. Alternatively, a magnetic rod and a magnetic rod sleeve are used in combination to achieve separation. Both of these methods are cumbersome and cannot easily and quickly separate the magnetic beads.

[0004] Application Contents

[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the present application is to provide a pipetting element for solving the problems in the prior art.

[0006] To achieve the above-mentioned purpose and other related purposes, the present application provides a pipetting element, which includes a main body and an adsorption part arranged in the main body, the main body includes a connecting section, a liquid storage section and a liquid aspiration section connected in sequence, the connecting section is used to adapt to the pipette, and the adsorption part is a magnetic adsorption part.

[0007] Preferably, the adsorption element is arranged in the liquid storage section or in the liquid absorption section, or at the junction of the liquid storage section and the liquid absorption section.

[0008] Preferably, the adsorption element is a column.

[0009] Preferably, the adsorption member is a magnet.

[0010] Preferably, the two magnetic poles of the magnet are parallel or perpendicular to the radial direction of the body.

[0011] Preferably, the adsorption element is provided with a liquid hole.

[0012] Preferably, the liquid through hole is parallel to the axial direction of the body (ie, the extension direction of the body).

[0013] Preferably, the outer diameter of the adsorbent is the same as the inner diameter of the liquid storage section or the inner diameter of the liquid absorption section, or the inner diameter of the junction between the liquid storage section and the liquid absorption section.

[0014] Preferably, a recessed portion is further provided on the adsorption member.

[0015] Preferably, the recessed portion is a groove parallel to the axial direction of the body.

[0016] Preferably, a support member is provided in the main body, and the support member is connected to the inner wall of the main body.

[0017] Preferably, a support platform is provided on the support member.

[0018] As described above, the liquid transfer element of the present application has the following beneficial effects: it can simply and quickly separate magnetic beads from liquids, is low-cost, and is convenient for automation, which is conducive to improving experimental efficiency. Moreover, it can realize molecular detection, immunoassay, separation and detection of macromolecules or small molecules such as proteins and peptides, depending on the substances bound to the surface of the separated magnetic beads, that is, it can realize a variety of applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] FIG1 is a schematic cross-sectional view of a pipetting element according to the present application.

[0020] FIG2 is a three-dimensional schematic diagram of the pipetting element of the present application.

[0021] FIG. 3 shows a front view of the pipetting element of the present application.

[0022] FIG. 4 shows one of the cross-sectional views of the pipetting element of the present application.

[0023] FIG5 is a partial enlarged view of the adsorption part of the pipetting element of the present application.

[0024] FIG. 6 shows one of the top views of the pipetting element of the present application (excluding the adsorbent).

[0025] FIG. 7 shows one of the top views of the pipetting element of the present application (including the adsorption component).

[0026] FIG8 shows one of the schematic diagrams of the top view and the side view of the adsorption component of the present application.

[0027] Component Reference Numerals 1 Main body 11 Connecting section 12 Liquid storage section 13 Liquid absorption section 2 Adsorption member 21 Liquid hole 22 Recessed portion 3 Support member 31 Support platform DETAILED DESCRIPTION

[0028] The following describes the implementation of the present application through specific embodiments. People familiar with this technology can easily understand other advantages and effects of the present application from the contents disclosed in this specification.

[0029] Please refer to Figures 1 to 8. It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the restrictive conditions for the implementation of this application. Therefore, they have no technical substantive significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed in this application without affecting the efficacy and purpose that can be achieved by this application. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" cited in this specification are only for the convenience of description and are not used to limit the scope of the implementation of this application. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of this application without substantially changing the technical content.

[0030] As shown in Figure 1, the present application provides a pipetting element, which includes a main body 1 and an adsorption component 2 arranged in the main body 1. The main body 1 is conical and includes a connecting section 11, a liquid storage section 12 and a liquid aspiration section 13 that are connected in sequence. The connecting section 11 is used to adapt to the pipette, and the adsorption component 2 is a magnetic adsorption component.

[0031] The body 1 is tapered, the connecting section 11 is arranged at the thick end of the tapered portion, and the liquid absorbing section is arranged at the thin end of the tapered portion.

[0032] In certain embodiments of the present application, the pipetting element is a pipette tip.

[0033] The body 1 is a conical structure with a cavity. In some embodiments of the present application, the body 1 is conical.

[0034] The inner diameter of the connecting section 11 is adapted to the pipette element mounting portion of the pipette. The pipette can be any device that can push air out of the pipette element after being connected to the pipette element, such as a pipette gun. The end of the liquid suction section 13 is provided with a liquid hole. The liquid hole is used to absorb liquid or release liquid from the pipette element.

[0035] In certain embodiments of the present application, a filter element may be further provided in the body 1. In a preferred embodiment, the filter element is provided in the connecting section 11 and / or the liquid storage section 12.

[0036] The adsorption element 2 is disposed in the liquid storage section 12 or in the liquid absorption section 13 , or at the junction of the liquid storage section 12 and the liquid absorption section 13 .

[0037] The number of the adsorption components 2 is not specifically limited, and may be one, two, three or more, for example.

[0038] The adsorption member 2 is a column.

[0039] In the embodiments of the present application as shown in Figures 1 to 7, the adsorption member 2 is a cylinder.

[0040] In certain embodiments of the present application, the adsorption member 2 is a magnet.

[0041] The two magnetic poles of the magnet are parallel or perpendicular to the radial direction of the body 1 .

[0042] In certain preferred embodiments of the present application, the line connecting the two poles of the magnet is perpendicular to the extension direction of the body 1 (when the body 1 is conical, the extension direction of the body 1 is axial, that is, the line connecting the two poles of the magnet is parallel to the radial direction of the body 1). The advantages of this design are a larger surface area for adsorption and, most importantly, no clogging of the pipetting element.

[0043] In the preferred embodiment shown in Figures 1 to 7, a support member 3 is provided in the body 1. The support member 3 is preferably fixedly connected to the inner wall of the body 1. The support member 3 is used to fix and support the adsorption member 2. The direction of the support member 3 is parallel to the axial direction of the body 1.

[0044] As shown in Figure 6, a support platform 31 is provided on the support member 3. Specifically, the support platform 31 is provided on the opposite surface of the support member 3 where it is connected to the body 1.

[0045] There are multiple support members 3. The adsorption member 2 is mounted on a support platform 31 on multiple support members 3. There are, for example, 2 to 4 support members 3. In the embodiment of the present application as shown in Figures 1 to 7, there are 3 support members 3.

[0046] The combination of the support member 3 and the cylindrical adsorption member can simplify the production process of the pipetting element.

[0047] In the embodiment of the present application as shown in FIG8 , the adsorption member 2 is provided with a liquid through hole 21. The liquid through hole 21 is used for liquid to pass through.

[0048] The liquid through hole 21 is parallel to the axial direction of the body 1. The shape of the liquid through hole 21 is not specifically limited, and can be, for example, circular or square.

[0049] The number of the liquid-through holes 21 is not specifically limited, and may be, for example, one (as shown in FIG8 ), two, three, four, five or more.

[0050] The diameter of the liquid through hole 21 is 0.3-0.6 mm, for example, 0.3-0.4 mm, 0.4-0.5 mm, or 0.5-0.6 mm.

[0051] In the embodiment of the present application as shown in FIG8 , the outer diameter of the adsorbent 2 is the same as the inner diameter of the liquid storage section 12 or the inner diameter of the liquid absorption section 13, or the inner diameter of the junction of the liquid storage section 12 and the liquid absorption section 13. The same inner diameter allows the adsorbent 2 to be fixed at a specific position within the body 1, for example, at the liquid storage section 12 or the liquid absorption section 13, or at the junction of the liquid storage section 12 and the liquid absorption section 13, so that it cannot move within the body 1.

[0052] In the embodiment shown in FIG. 8 , a recessed portion 22 is further provided on the adsorption member 2 .

[0053] In certain embodiments of the present application, the recessed portion 22 is a groove parallel to the axial direction of the body 1, that is, in the same direction as the liquid flow direction. The recessed portion 22 is designed to prevent the pipetting element from being blocked during use.

[0054] The size or other features of the pipetting element of the present application can be designed according to the size of conventional pipette tips in the prior art, and this application does not make any specific limitations.

[0055] The specifications of the pipetting element of the present application can be 10 μL, 20 μL, 100 μL, 200 μL, 300 μL, 1000 μL, 1250 μL, 5 mL or 10 mL.

[0056] In certain embodiments of the present application, the pipetting element may be made of polypropylene material and is free of DNase, RNase, pyrogen, human DNA, and RNA.

[0057] In certain embodiments of the present application, the pipetting element may be sterilized or not.

[0058] The present application also provides the use of the pipetting element in magnetic bead separation, or in the preparation of nucleic acid extraction or detection products, products for immune response, or products for removing impurities.

[0059] The nucleic acid detection product or the immune response product can be determined according to the different surface properties of the magnetic beads. For example, when the surface of the magnetic beads is silica, it is generally used for nucleic acid extraction or nucleic acid detection; for example, when the surface of the magnetic beads is coupled with proteins, antibodies or affinity groups, it can be used for antigen detection or for separating, concentrating and purifying proteins.

[0060] The pipetting element of the present application can be used to carry magnetic beads to participate in reactions. That is, after the magnetic beads are adsorbed on the adsorption member 2, the body 1 of the pipetting element can be used as a reaction container, and other reagents can be absorbed or released to react with the substances on the magnetic beads.

[0061] The present application also provides a magnetic bead separation method, which includes installing the pipetting element on a pipette, and using the pipette to repeatedly absorb and release the liquid containing the magnetic beads to separate the magnetic beads from the liquid.

[0062] The method of using the pipetting element of this application is as follows:

[0063] The pipetting element of the present application is mounted on a pipette, and the liquid containing magnetic microspheres is sucked up, and then the sucked liquid is slowly released. The magnetic microspheres can be adsorbed on the adsorption member 2 in the pipetting element. The suction and release are repeated until the liquid becomes clear and all the magnetic microspheres are adsorbed on the adsorption member 2. The pipetting element with adsorbed magnetic microspheres can be further processed or reacted with the adsorbed magnetic microspheres by sucking up and releasing other liquids for purposes such as detection and washing, depending on the different substances bound to the surface of the magnetic microspheres. In this case, the pipetting element also serves as a reaction container, greatly simplifying the processing process of the magnetic beads.

[0064] In summary, the present invention effectively overcomes various shortcomings of the prior art and has high industrial utilization value.

[0065] The above embodiments are merely illustrative of the principles and effects of this application and are not intended to limit this application. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of this application. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical concepts disclosed in this application shall be covered by the claims of this application.

Claims

1. A liquid transfer element, characterized in that: The liquid transfer element comprises a main body (1) and an adsorption member (2) arranged in the main body (1); the main body (1) comprises a connecting section (11), a liquid storage section (12) and a liquid suction section (13) which are connected in sequence; the connecting section (11) is used to adapt to the pipette; and the adsorption member (2) is a magnetic adsorption member.

2. The liquid transfer element according to claim 1, characterized in that The main body (1) is in a cone shape, the connecting section (11) is arranged at the thick end of the cone, and the liquid suction section is arranged at the thin end of the cone.

3. The liquid transfer element according to claim 1, characterized in that The body (1) is a conical structure with a cavity; and / or the end of the liquid suction section (13) is provided with a liquid hole.

4. The liquid transfer element according to claim 1, characterized in that The adsorbent (2) is arranged in the liquid storage section (12) or in the liquid absorption section (13), or at the junction of the liquid storage section (12) and the liquid absorption section (13).

5. The liquid transfer element according to claim 1, characterized in that: The number of the adsorption components (2) is one or more, and / or the adsorption components (2) are columns.

6. The liquid transfer element according to claim 1, characterized in that: The adsorption element (2) is a magnet.

7. The liquid transfer element according to claim 6, characterized in that The two magnetic poles of the magnet are parallel or perpendicular to the extension direction of the body (1).

8. The liquid transfer element according to claim 1, characterized in that: The adsorbent (2) is provided with a liquid through hole (21).

9. The liquid transfer element according to claim 8, characterized in that The liquid through holes (21) are parallel to the extension direction of the body (1), and / or the number of the liquid through holes (21) is 1 to 6.

10. The liquid transfer element according to claim 8, characterized in that The maximum outer diameter of the adsorbent (2) is the same as the inner diameter of the liquid storage section (12) or the inner diameter of the liquid absorption section (13), or the inner diameter of the junction between the liquid storage section (12) and the liquid absorption section (13); and / or, the adsorbent (2) is also provided with a recessed portion (22), and the recessed portion (22) is a groove parallel to the extension direction of the main body (1).

11. The liquid transfer element according to claim 1, characterized in that: A support member (3) is provided inside the body (1); one side of the support member (3) is connected to the inner wall of the body (1), and the other side of the support member (3) is provided with a support platform (31) for mounting the adsorption member (2).

12. The liquid transfer element according to claim 11, characterized in that The supporting members (3) are provided in plurality.

13. Use of the liquid transfer element according to any one of claims 1 to 12 in magnetic bead separation, or in the preparation of nucleic acid extraction or detection products, products for immune response or products for removing impurities, or in carrying magnetic beads to participate in reactions.

14. A magnetic bead separation method, characterized in that: The magnetic bead separation method comprises installing the liquid transfer element according to any one of claims 1 to 12 on a liquid transfer device, and using the liquid transfer device to repeatedly suck up and release the liquid containing the magnetic beads, thereby separating the magnetic beads from the liquid.

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