Oral bacteria reduction agent, oral bacteria reduction dentifrice, oral bacteria reduction mouthwash, oral bacteria reduction gum, oral bacteria reduction method, periodontal disease ameliorating agent, periodontal disease ameliorating dentifrice, periodontal disease ameliorating mouthwash, periodontal disease ameliorating gum, and periodontal disease ameliorating method

Abstract

The present invention mainly addresses the problem of providing an oral bacteria reduction agent and a periodontal disease ameliorating agent that contain an active ingredient that does not pose a problem even if absorbed into the body through the oral cavity over the long term and that can reduce the counts of multiple bacterium that cause periodontal diseases.　The oral bacteria reduction agent and the periodontal disease ameliorating agent according to the present invention are characterized by containing, as an active ingredient, a seaweed powder, which is a powder obtained by drying and pulverizing seaweed or is a powder obtained by drying an extract of the seaweed, and by containing at least 0.01 mass% iodine.

Description

Oral fungus reducing agent, oral fungus reducing toothpaste, oral fungus reducing mouthwash, oral fungus reducing gum, oral fungus reducing method, periodontal disease improving agent, periodontal disease improving toothpaste, periodontal disease improving mouthwash, periodontal disease improving gum, and periodontal disease improving method

[0001] The present invention relates to an oral fungus-reducing agent, an oral fungus-reducing dentifrice, an oral fungus-reducing mouthwash, an oral fungus-reducing gum, and a method for reducing oral fungi, each containing the oral fungus-reducing agent.The present invention also relates to a periodontal disease-reducing agent, a periodontal disease-reducing dentifrice, a periodontal disease-reducing mouthwash, a periodontal disease-reducing gum, and a method for reducing periodontal disease, each containing the periodontal disease-reducing agent.

[0002] There are said to be hundreds of types of bacteria living in the oral cavity, and among them, Porphyromonas gingivalis (P. g) is well known as one of the multiple causative bacteria of periodontal disease. Periodontal disease is a chronic disease in which the causative bacteria proliferate in plaque that adheres to the periodontal pocket, which is the groove between the gums and teeth, and excrete toxins, which destroy the periodontal pocket and deepen it.

[0003] Recently, toothpastes (tooth powder, liquid toothpaste, etc.) and mouthwashes (dental rinse, mouthwash, etc.) that are effective in preventing periodontal disease by removing bacteria that cause periodontal disease from the oral cavity have been commercially available. However, toothpastes and mouthwashes widely sold in Japan and abroad often contain bactericides such as 1,3,3-trimethyl (1,8-cineole), thymol, methyl salicylate, and cetylpyridinium chloride, which are originally disinfectants that suppress the growth of bacteria that cause periodontal disease, as well as dipotassium glycyrrhizinate, which has a soothing effect on inflammation in the gums and mouth. Some toothpastes and mouthwashes also contain antibiotics that inhibit the growth of certain bacteria. However, the ingredients of these bactericides, antibiotics, etc. are not originally intended for daily consumption and are absorbed into the body via the oral cavity, which presents a long-term problem.

[0004] Furthermore, even if the ingredients of bactericides (antibacterial agents) for bacteria that cause periodontal disease are themselves natural products, they are not normally edible, or the antibacterial agent ingredients are not water-soluble and therefore need to be dissolved in non-natural (artificially synthesized) solvents, or artificial sweeteners are sometimes used in combination as auxiliary agents for the antibacterial agent (see Patent Document 1, etc.). Even these water-insoluble / slightly soluble natural bactericides (antibacterial agents) require the incorporation of non-natural solvents or additives in order to commercialize them, and they are absorbed directly or indirectly into the body, which is undesirable in the long term, posing a problem.

[0005] Furthermore, Patent Document 2 discloses that the seaweed extract has antibacterial activity specific to Porphyromonas gingivalis (P. g bacteria). However, because this antibacterial activity is expressed as the minimum inhibitory concentration (MIC, ppm), there is a problem in that the seaweed extract of Patent Document 2 inhibits growth but does not reduce the number of bacteria. Furthermore, while it has been shown that the antibacterial activity of a chloroform-methanol extract against Porphyromonas gingivalis (P. g bacteria) is higher than that of a water extract, the only other periodontal disease causative bacteria disclosed is Fusobacterium nucleatum (F. n bacteria), and antibacterial activity against Fusobacterium nucleatum (F. n bacteria) has not been confirmed. In other words, there is a problem in that some of the seaweed extracts of Patent Document 2 do not have antibacterial activity against multiple periodontal disease causative bacteria. Furthermore, since the extraction residue must be removed during the extraction process of seaweed extract, there was a problem in that it was not possible to produce an oral fungus-reducing agent that fully utilizes the components contained in the seaweed before the extraction process.

[0006] JP 2020-62006 JP 9-48715

[0007] The main objective of the present invention is to provide an oral fungus reducer and a periodontal disease improver that contain active ingredients that can be absorbed into the body via the oral cavity over a long period of time without causing any problems and that can reduce the number of bacteria that cause multiple periodontal disease.

[0008] As a result of intensive research in light of the above circumstances, the inventors have discovered an oral fungus reducer and periodontal disease improver that contain active ingredients that can be absorbed into the body via the oral cavity over the long term without causing any problems, and that can reduce the number of bacteria that cause multiple periodontal disease.

[0009] In other words, the oral fungus-reducing agent of the present invention is an oral fungus-reducing agent that contains seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of the seaweed, and contains 0.01 mass% or more of iodine, and the oral fungi are one or more selected from the group consisting of periodontal disease bacteria Porphyromonas gingivalis (P.g bacteria), Treponema denticola (T.d bacteria), Tannerella forsythia (T.f bacteria), Aggregatibacter actinomycetemcomitans (A.a bacteria), and Fusobacterium nucleatum (F.n bacteria), and is characterized in that the number of oral fungi is reduced by spreading the oral fungus-reducing agent throughout the oral cavity.

[0010] The oral bacteria are preferably Porphyromonas gingivalis (P. g), and the ratio of the number of Porphyromonas gingivalis (P. g) bacteria to the total number of bacteria in the oral cavity is desirably reduced to 0.49% or less.

[0011] The oral bacteria are Treponema denticola (T. d), and it is desirable that the ratio of the number of Treponema denticola (T. d) bacteria to the total number of bacteria in the oral cavity is reduced to 0.49% or less.

[0012] The oral fungi are Tannerella forsythia (T. f), and it is desirable that the ratio of the number of Tannerella forsythia (T. f) bacteria to the total number of bacteria in the oral cavity be reduced to 0.49% or less.

[0013] The oral fungi may all be selected from the group consisting of Porphyromonas gingivalis (P. g), Treponema denticola (T. d), and Tannerella forsythia (T. f), and the agent may simultaneously reduce the ratio of Porphyromonas gingivalis (P. g) to 0.49% or less, the ratio of Treponema denticola (T. d) to 0.49% or less, and the ratio of Tannerella forsythia (T. f) to 0.49% or less, relative to the total number of bacteria in the oral cavity.

[0014] The seaweed powder preferably has a pH of 5 or more and 8 or less.

[0015] It may further comprise iodine powder and / or zinc powder.

[0016] The iodine powder is preferably present in an amount of 0.01% by mass or more and less than 100% by mass relative to the mass of the powder obtained from the seaweed powder.

[0017] The composition may further contain one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder.

[0018] The oral fungus-reducing dentifrice of the present invention is prepared by incorporating the oral fungus-reducing agent as an active ingredient into toothpaste or liquid toothpaste.

[0019] The oral fungus-reducing mouthwash of the present invention is a mouthwash solution containing the oral fungus-reducing agent as an active ingredient.

[0020] The oral fungus-reducing gum of the present invention is a gum containing the oral fungus-reducing agent as an active ingredient.

[0021] The method for reducing oral bacteria of the present invention is characterized by comprising the steps of spreading the oral bacteria-reducing agent throughout the oral cavity and repeating the step of spreading the agent throughout the oral cavity for a certain period of time.

[0022] The step of distributing the agent in the oral cavity desirably includes a step of distributing 0.01 g or more of the agent for reducing oral fungi in the oral cavity per day.

[0023] The step of irrigating the oral cavity may include using an ultrasonic toothbrush.

[0024] The periodontal disease improving agent of the present invention is a periodontal disease improving agent containing seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of the seaweed, and contains 0.01 mass% or more of iodine.By distributing the oral bacteria reducing agent throughout the oral cavity, periodontal disease is improved.

[0025] The periodontal disease-improving dentifrice of the present invention is a toothpaste or liquid toothpaste containing a periodontal disease-improving agent as an active ingredient.

[0026] The periodontal disease-improving mouthwash of the present invention is a mouthwash solution containing a periodontal disease-improving agent as an active ingredient.

[0027] The periodontal disease-ameliorating gum of the present invention is a gum containing a periodontal disease-ameliorating agent as an active ingredient.

[0028] The method for improving periodontal disease of the present invention comprises the steps of spreading a periodontal disease improving agent throughout the oral cavity and repeating the step of spreading the agent throughout the oral cavity for a certain period of time.

[0029] The step of spreading the agent throughout the oral cavity preferably includes a step of spreading 0.01 g or more of the periodontal disease improving agent throughout the oral cavity per day.

[0030] The step of irrigating the oral cavity may include using an ultrasonic toothbrush.

[0031] The present invention can provide an oral fungus reducer and / or periodontal disease improver that can reduce the number of multiple bacteria that cause periodontal disease without causing any problems even if the active ingredient is absorbed into the body via the oral cavity over a long period of time.

[0032] Graph showing periodontal disease bacteria PCR results. Graph showing reduction of Porphyromonas gingivalis (P. g bacteria) by kelp powder. Graph showing reduction of Fusobacterium nucleatum (F. n bacteria) by kelp powder. Graph showing reduction of Tannerella forsythia (T. f bacteria) by kelp powder. Graph showing reduction of Treponema denticola (T. d bacteria). Photographs showing an example of a method for spreading an oral fungus-reducing agent and / or a periodontal disease-improving agent throughout the oral cavity.

[0033] The embodiments of the present invention will be specifically described below.

[0034] (Oral fungus-reducing agent) The oral fungus-reducing agent of the present invention contains seaweed powder as an active ingredient. The oral fungus-reducing agent is an agent that has the effect of reducing the number of bacteria in the oral cavity, particularly the number of fungi distributed in periodontal pockets. The reduction in the number of fungi is thought to be mainly due to the effect of sterilization, but the contribution of the antibacterial effect that prevents the proliferation of bacteria cannot be denied.

[0035] (Seaweed Powder) The seaweed powder is a powder obtained by drying and pulverizing seaweed or a powder obtained by drying an extract of seaweed.

[0036] When seaweed is dried and crushed to obtain a powder, theoretically, all components of the seaweed except for water and volatile substances can be contained in the powder. Here, the drying temperature is preferably a temperature similar to that used for drying food (e.g., about 60°C). The drying time is adjusted to the point where there is almost no weight loss of the dried product if the residual moisture content is to be close to zero, and shortened if some moisture is to be retained. The crushing time is adjusted depending on the particle size of the resulting powder.

[0037] When seaweed is extracted and the extract is dried to obtain a powder, the extraction residue is not contained in the powder. The components of the resulting powder vary depending on the extraction solvent, extraction temperature, extraction time, etc. used in the extraction process. In particular, the components that are soluble and dissolve in the extract vary depending on the selection of the extraction solvent. Examples of extraction solvents include water, alcohol, organic solvents, and mixtures thereof. In addition, a drying and grinding process may be included before the extraction process to improve extraction efficiency.

[0038] The seaweed powder contains a certain amount or more of iodine as a component of the seaweed powder. The iodine content is 0.01 mass% or more, preferably 0.1 mass% or more, and more preferably 0.2 mass% or more. Examples of seaweed containing a certain amount or more of iodine include kelp, hijiki, and wakame. To ensure that the seaweed powder contains a certain amount or more of iodine, it is preferable to select seaweed with a high iodine content and dry and grind the seaweed to obtain a powder so that the iodine is contained in the powder. Note that even when drying a seaweed extract to obtain a powder, the extraction conditions may be such that iodine can be specifically extracted.

[0039] The pH of the seaweed powder is preferably 5 or more and 8 or less, more preferably 5.5 or more and 7 or less. The pH of the seaweed powder is measured by dissolving a certain amount in water. Because it is for repeated oral use, if the pH becomes acidic, below 5, there is a high possibility that it will damage the mucous membrane in the oral cavity or dissolve the tooth surface due to acid erosion. If the pH becomes alkaline, above 8, it will also be more likely to damage the mucous membrane in the oral cavity.

[0040] Considering the pH range of seaweed powder, it is thought that the iodine contained in seaweed powder does not form the structure of povidone-iodine (isodine), which is known for its high bactericidal properties, i.e., a complex of polyvinylpyrrolidone and iodine. The pH of povidone-iodine in a 1% aqueous solution is known to be approximately 1.5 to 3.5.

[0041] The particle size of the seaweed powder is appropriately set so as to spread throughout the oral cavity, and is, for example, about 5 μm to 50 μm. Because seaweed powder contains components that are difficult to dissolve in water, a finer particle size is preferable in order to spread it throughout the oral cavity, such as in periodontal pockets.

[0042] (Oral fungi) The oral fungi are one or more selected from the group consisting of periodontal disease bacteria Porphyromonas gingivalis (P. g), Treponema denticola (T. d), Tannerella forsythia (T. f), Aggregatibacter actinomycetemcomitans (A. a), and Fusobacterium nucleatum (F. n).

[0043] Porphyromonas gingivalis (P. g) is a bacterium detected in periodontal disease, especially severe periodontal disease. It correlates with gingival inflammation and is a typical bacterium that inhabits the depths of periodontal pockets. Various methods are available for assessing periodontal disease. For example, a high risk of periodontal disease is determined when the ratio of P. gingivalis (P. g) bacteria to the total number of bacteria collected in the oral cavity exceeds 0.49%. Therefore, the ratio of P. gingivalis (P. g) bacteria to the total number of bacteria collected in the oral cavity is preferably 0.49% or less, more preferably 0.1% or less, and even more preferably below the detection limit (e.g., 10 copies or less, depending on the test system). One method for collecting oral bacteria is to insert a dental paper point into a periodontal pocket, hold it there for a predetermined number of seconds, and then remove it. The bacteria in the liquid collected by the paper point are amplified using real-time PCR, and the copy number is counted. When PCR testing is performed using this method, as an example of a determination, if the number of Porphyromonas gingivalis (P. g bacteria) exceeds 1,000 copies, it is determined that the risk of periodontal disease is high. Therefore, the amplified number of Porphyromonas gingivalis (P. g bacteria) is preferably 1,000 copies or less, more preferably 100 copies or less, and even more preferably below the detection limit (e.g., 10 copies or less).

[0044] Treponema denticola (T. d) is a type of spiral-shaped spirochete that inhabits periodontal pockets, plaque, and tongue coating. Various methods are available for assessing periodontal disease. For example, a high risk of periodontal disease is determined when the ratio of Treponema denticola (T. d) bacteria to the total number of bacteria collected in the oral cavity exceeds 0.49%. Therefore, the ratio of Treponema denticola (T. d) bacteria to the total number of bacteria collected in the oral cavity is preferably 0.49% or less, more preferably 0.1% or less, and even more preferably below the detection limit (e.g., 10 copies or less). One method for collecting bacteria in the oral cavity involves inserting a dental paper point into a periodontal pocket, holding it there for a predetermined number of seconds, and then removing it. The bacteria in the liquid collected with the paper point are amplified using real-time PCR, and the copy number is counted. When PCR testing is performed using this method, as an example of the determination, if the number of Treponema denticola (T. d) bacteria exceeds 1,000 copies, it is determined that the risk of periodontal disease is high. Therefore, the amplified number of Treponema denticola (T. d) bacteria is preferably 1,000 copies or less, more preferably 100 copies or less, and even more preferably below the detection limit (e.g., 10 copies or less).

[0045] Tannerella forsythia (T. f.) is a spindle-shaped bacterium that inhabits plaque and tartar. It is an indicator of intractable periodontitis and is believed to destroy the gums by releasing toxins and enzymes. Various methods are available for assessing periodontal disease. For example, a high risk of periodontal disease is determined when the ratio of Tannerella forsythia (T. f.) bacteria to the total number of bacteria collected in the oral cavity exceeds 0.49%. Therefore, the ratio of Tannerella forsythia (T. f.) bacteria to the total number of bacteria collected in the oral cavity is preferably 0.49% or less, more preferably 0.1% or less, and even more preferably below the detection limit (e.g., 10 copies or less). One method for collecting oral bacteria is to insert a dental paper tip into a periodontal pocket, hold it for a predetermined number of seconds, and then remove it. The bacteria in the liquid collected with the paper tip are amplified using real-time PCR, and the copy number is counted. When PCR testing is performed using this method, as an example of the determination, if the number of Tannerella forsythia (T. f) bacteria exceeds 1,000 copies, it is determined that there is a high risk of periodontal disease. Therefore, it is preferable that the number of amplified Tannerella forsythia (T. f) bacteria is 1,000 copies or less, more preferably 100 copies or less, and even more preferably below the detection limit (e.g., 10 copies or less).

[0046] Aggregatibacter actinomycetemcomitans (A. a bacteria) is involved in severe aggressive periodontitis and releases toxins that inflame the gums and dissolve the bone supporting the teeth. One example of a method for collecting oral bacteria is to insert a dental paper tip into a periodontal pocket, hold it there for a predetermined number of seconds, and then remove it. The bacteria in the liquid collected with the paper tip are amplified using real-time PCR, and the copy number is counted. When PCR testing is performed using this method, for example, if one or more copies of Aggregatibacter actinomycetemcomitans (A. a bacteria) are detected, it is determined that the risk of periodontal disease is high. Therefore, it is even more preferable that Aggregatibacter actinomycetemcomitans (A. a bacteria) be zero copies (not detected) even after PCR amplification.

[0047] Fusobacterium nucleatum (F.n) is a bacterium that causes bad breath and is involved in the formation of dental plaque. One method for collecting oral bacteria is to insert a dental paper tip into a periodontal pocket, hold it there for a predetermined number of seconds, and then remove it. The bacteria in the liquid collected with the paper tip are amplified using real-time PCR, and the copy number is counted. When PCR testing is performed using this method, as an example of a determination, if the number of Fusobacterium nucleatum (F.n) exceeds 1,000 copies, the risk of periodontal disease is determined to be high. Therefore, it is preferable that the amplified number of Fusobacterium nucleatum (F.n) is 1,000 copies or less, more preferably 400 copies or less, 100 copies or less, and even more preferably below the detection limit (e.g., 10 copies or less).

[0048] The oral fungi are all selected from the group consisting of Porphyromonas gingivalis (P. g), Treponema denticola (T. d), and Tannerella forsythia (T. f), and it is desirable that the oral fungus reducer simultaneously reduces the ratio of Porphyromonas gingivalis (P. g) bacteria to 0.49% or less, the ratio of Treponema denticola (T. d) bacteria to 0.49% or less, and the ratio of Tannerella forsythia (T. f) bacteria to 0.49% or less, relative to the total number of bacteria in the oral cavity. In this case, it is more desirable that the oral fungus reducer simultaneously reduces the ratio of Porphyromonas gingivalis (P. g) bacteria to the total number of bacteria in the oral cavity to below the detection limit (0%), reduces the ratio of Treponema denticola (T. d) bacteria to below the detection limit (0%), and simultaneously reduces the ratio of Tannerella forsythia (T. f) bacteria to below the detection limit (0%).

[0049] Furthermore, the oral fungi are all selected from the group consisting of Porphyromonas gingivalis (P. g), Treponema denticola (T. d), Tannerella forsythia (T. f), Aggregatibacter actinomycetemcomitans (A. a), and Fusobacterium nucleatum (F. n), and the oral fungus-reducing agent is a fungus-reducing agent for reducing the number of Porphyromonas gingivalis (P. g) bacteria relative to the total number of bacteria in the oral cavity. It is desirable to simultaneously reduce the ratio of Treponema denticola (T. d) bacteria to 0.49% or less, reduce the ratio of Tannerella forsythia (T. f) bacteria to 0.49% or less, not detect Aggregatibacter actinomycetemcomitans (A. a) bacteria, and reduce the number of Fusobacterium nucleatum (F. n) bacteria to 1,000 copies or less. In this case, it is even more desirable to reduce the ratio of Porphyromonas gingivalis (P. g bacteria) to below the detection limit (0%), the ratio of Treponema denticola (T. d bacteria) to below the detection limit (0%), the ratio of Tannerella forsythia (T. f bacteria) to below the detection limit (0%), and not detect Aggregatibacter actinomycetemcomitans (A. a bacteria), and simultaneously reduce the number of Fusobacterium nucleatum (F. n bacteria) to 400 copies or below the detection limit (10 copies or below).

[0050] (Application to the Oral Cavity) The oral fungus-reducing agent is applied so as to be distributed throughout the oral cavity. For example, as shown in FIG. 6( a), an appropriate amount of the oral fungus-reducing agent can be applied to a toothbrush and then brushed to distribute the agent throughout the oral cavity. As shown in FIG. 6( b), an appropriate amount of the oral fungus-reducing agent can be applied to toothpaste on a toothbrush and then brushed to distribute the agent throughout the oral cavity. As shown in FIG. 6( c), an appropriate amount of toothpaste containing the oral fungus-reducing agent can be applied to a toothbrush and then brushed to distribute the agent throughout the oral cavity. In addition, an appropriate amount of the oral fungus-reducing agent can be added to a mouthwash and then rinsing the mouth to distribute the agent throughout the oral cavity. Alternatively, an appropriate amount of the oral fungus-reducing agent can be added to gum and then chewed to distribute the agent throughout the oral cavity. The amount of oral bacteria-reducing agent used can be adjusted appropriately depending on the time and frequency of brushing, but it is desirable to use 0.01 g or more per day to ensure that it is distributed throughout the oral cavity, and it is even more desirable to use 0.1 g or more.

[0051] (Other Ingredients) The oral fungus-reducing agent contains seaweed powder as an active ingredient, but because seaweed is collected from nature, the oral fungus-reducing effect may vary depending on individual differences, regional differences, seasonal differences, powder processing conditions, etc., as well as the conditions under which the oral fungus-reducing agent is distributed throughout the oral cavity. Therefore, if necessary, an auxiliary agent that has the oral fungus-reducing effect even when used alone can be added to the seaweed powder.

[0052] The oral fungus-reducing agent may further contain iodine powder and / or zinc powder as an auxiliary agent. Unlike povidone-iodine, iodine powder has a pH of approximately 4.5, and as described in the Examples, it also has a high fungus-reducing effect on periodontal disease bacteria. Therefore, even a small amount of iodine powder can function as an auxiliary agent. The amount can be, for example, 0.01% by mass or more but less than 100% by mass, or even 0.01% by mass or more but less than 50% by mass, relative to the mass of the seaweed powder. Iodine powder itself tends to reduce periodontal disease bacteria in a short period of time (e.g., within 1 minute), and can be added as an auxiliary agent. As described in the Examples, zinc powder has a fungus-reducing effect on periodontal disease bacteria, and therefore, even a small amount can function as an auxiliary agent. The amount of zinc powder added can be, for example, 0.1% by mass or more but less than 40% by mass, relative to the mass of the seaweed powder.

[0053] The additive may further include one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder.Further, the additive may include laminaran powder, ascophyllum nodosum powder, ascorafen powder, β-carotene powder, and laver micron powder.

[0054] (Oral Bacteria-Reducing Dentifrice) An oral bacteria-reducing dentifrice is a toothpaste or liquid toothpaste in which an oral bacteria-reducing agent is blended as an active ingredient. An appropriate amount of oral bacteria-reducing agent is blended so that the oral bacteria-reducing agent is distributed throughout the oral cavity when brushing teeth with the oral bacteria-reducing dentifrice. As shown in Figure 6(c), the oral bacteria-reducing dentifrice is applied to a toothbrush to a depth of about 1 cm to 3 cm when brushing teeth.

[0055] (Oral fungus-reducing mouthwash) The oral fungus-reducing mouthwash is a mouthwash solution containing an oral fungus-reducing agent as an active ingredient. An appropriate amount of the oral fungus-reducing agent is blended so that the oral fungus-reducing agent is distributed throughout the oral cavity by rinsing with the oral fungus-reducing mouthwash.

[0056] (Oral fungus-reducing gum) The oral fungus-reducing gum is a gum containing an oral fungus-reducing agent as an active ingredient. An appropriate amount of the oral fungus-reducing agent is blended so that the oral fungus-reducing agent is distributed throughout the oral cavity by chewing the oral fungus-reducing gum.

[0057] (Method for Reducing Oral Fungi) The method for reducing oral fungi includes a step of spreading an oral fungi-reducing agent throughout the oral cavity, and a step of repeating the step of spreading the agent throughout the oral cavity for a certain period of time.

[0058] The process of spreading the oral fungus-reducing agent throughout the oral cavity can be carried out, for example, by applying an appropriate amount of the oral fungus-reducing agent to a toothbrush and brushing the teeth, as shown in Figure 6(a). As shown in Figure 6(b), it is also possible to spread the oral fungus-reducing agent throughout the oral cavity by applying an appropriate amount of toothpaste to a toothbrush and then brushing the teeth. As shown in Figure 6(c), it is also possible to spread the oral fungus-reducing agent throughout the oral cavity by applying an appropriate amount of toothpaste containing the oral fungus-reducing agent to a toothbrush and brushing the teeth. Alternatively, it is also possible to add an appropriate amount of the oral fungus-reducing agent to a mouthwash and then rinsing the mouth to spread the agent throughout the oral cavity. It is also possible to add an appropriate amount of the oral fungus-reducing agent to gum and then chew the gum to spread the agent throughout the oral cavity. The amount of oral bacteria reducer used is adjusted appropriately depending on the tooth brushing time and frequency, mouth rinsing time and frequency, gum chewing time and frequency, etc., but it is desirable to use 0.01 g or more per day to distribute throughout the oral cavity, and more desirable to use 0.1 g or more.

[0059] An ultrasonic toothbrush can be used in the process of spreading the oral fungus-reducing agent throughout the oral cavity. Ultrasound refers to sound waves of 20 kHz or higher, and ultrasonic toothbrushes typically use a frequency of approximately 1.6 MHz to 2.0 MHz. It is more preferable to apply ultrasonic vibrations to the ultrasonic toothbrush. Examples of ultrasonic vibrations include approximately 16,000 strokes / minute to 20,000 strokes / minute.

[0060] The step of repeating the above-mentioned step of spreading the agent throughout the oral cavity for a certain period of time is carried out, for example, for about three months to one and a half years, or continuously thereafter, by repeating tooth brushing, mouth rinsing, chewing gum, etc., preferably every day, more preferably after each meal.

[0061] (Periodontal Disease Improving Agent) The periodontal disease improving agent of the present invention contains seaweed powder as an active ingredient. The periodontal disease improving agent improves periodontal disease, reduces the depth of gum pockets (periodontal pockets) to less than 4 mm, and further reduces the bleeding rate during a probing test. A probing test, also known as a gum pocket test, involves inserting a graduated probe into a gum pocket to measure the depth from the gum entrance to the bottom of the pocket while simultaneously checking for the presence or absence of bleeding. The test is performed at six locations per tooth. The depth of a gum pocket is approximately 1-2 mm in healthy gums, and a depth of 4 mm or greater is diagnosed as periodontal disease. The presence or absence of bleeding is calculated as the percentage of bleeding locations out of the total number of locations examined (for 32 teeth, 32 x 6 = 192 locations). A bleeding rate of 20% or less is considered preferable.

[0062] (Seaweed Powder) The seaweed powder is a powder obtained by drying and pulverizing seaweed or a powder obtained by drying an extract of seaweed.

[0063] When seaweed is dried and crushed to obtain a powder, theoretically, all components of the seaweed except for water and volatile substances can be contained in the powder. Here, the drying temperature is preferably a temperature similar to that used for drying food (e.g., about 60°C). The drying time is adjusted to the point where there is almost no weight loss of the dried product if the residual moisture content is to be close to zero, and shortened if some moisture is to be retained. The crushing time is adjusted depending on the particle size of the resulting powder.

[0064] When seaweed is extracted and the extract is dried to obtain a powder, the extraction residue is not contained in the powder. The components of the resulting powder vary depending on the extraction solvent, extraction temperature, extraction time, etc. used in the extraction process. In particular, the components that are soluble and dissolve in the extract vary depending on the selection of the extraction solvent. Examples of extraction solvents include water, alcohol, organic solvents, and mixtures thereof. In addition, a drying and grinding process may be included before the extraction process to improve extraction efficiency.

[0065] The seaweed powder contains a certain amount or more of iodine as a component of the seaweed powder. The iodine content is 0.01 mass% or more, preferably 0.1 mass% or more, and more preferably 0.2 mass% or more. Examples of seaweed containing a certain amount or more of iodine include kelp, hijiki, and wakame. To ensure that the seaweed powder contains a certain amount or more of iodine, it is preferable to select seaweed with a high iodine content and dry and grind the seaweed to obtain a powder so that the iodine is contained in the powder. Note that even when drying a seaweed extract to obtain a powder, the extraction conditions may be such that iodine can be specifically extracted.

[0066] The pH of the seaweed powder is preferably 5 or more and 8 or less, more preferably 5.5 or more and 7 or less. The pH of the seaweed powder is measured by dissolving a certain amount in water. Because it is for repeated oral use, if the pH becomes acidic, below 5, there is a high possibility that it will damage the mucous membrane in the oral cavity or dissolve the tooth surface due to acid erosion. If the pH becomes alkaline, above 8, it will also be more likely to damage the mucous membrane in the oral cavity.

[0067] Considering the pH range of seaweed powder, it is thought that the iodine contained in seaweed powder does not form the structure of povidone-iodine (isodine), which is known for its high bactericidal properties, i.e., a complex of polyvinylpyrrolidone and iodine. The pH of povidone-iodine in a 1% aqueous solution is known to be approximately 1.5 to 3.5.

[0068] The particle size of the seaweed powder is appropriately set so as to spread throughout the oral cavity, and is, for example, about 5 μm to 50 μm. Because seaweed powder contains components that are difficult to dissolve in water, a finer particle size is preferable in order to spread it throughout the oral cavity, such as to the gum pockets.

[0069] (Application to the Oral Cavity) The periodontal disease improving agent is applied so as to be distributed throughout the oral cavity. For example, as shown in FIG. 6( a), an appropriate amount of the periodontal disease improving agent can be applied to a toothbrush and then brushed to distribute throughout the oral cavity. As shown in FIG. 6( b), an appropriate amount of the periodontal disease improving agent can be applied to toothpaste on a toothbrush and then brushed to distribute throughout the oral cavity. As shown in FIG. 6( c), an appropriate amount of toothpaste containing the periodontal disease improving agent can be applied to a toothbrush and then brushed to distribute throughout the oral cavity. Alternatively, an appropriate amount of the periodontal disease improving agent can be added to a mouthwash and then rinsing the mouth to distribute throughout the oral cavity. Alternatively, an appropriate amount of the periodontal disease improving agent can be added to gum and then chewed to distribute throughout the oral cavity. The amount of periodontal disease improving agent used is adjusted appropriately depending on the time and frequency of tooth brushing, etc., but it is desirable to use 0.01 g or more per day to distribute throughout the oral cavity, and more desirable to use 0.1 g or more.

[0070] (Other Components) The periodontal disease improving agent contains seaweed powder as an active ingredient, but because seaweed is collected from nature, the periodontal disease improving effect may vary depending on individual differences, regional differences, seasonal differences, powder processing conditions, etc., as well as conditions for distributing the periodontal disease improving agent throughout the oral cavity. Therefore, if necessary, an auxiliary agent that has the effect of improving periodontal disease even when used alone can be added to the seaweed powder.

[0071] The periodontal disease improving agent may further contain iodine powder and / or zinc powder as an auxiliary agent. Unlike povidone-iodine, iodine powder has a pH of about 4.5, and as will be described later in the Examples, it also has a high fungus-reducing effect on periodontal bacteria. Therefore, even a small amount of iodine powder can function as an auxiliary agent. The amount can be, for example, 0.01% by mass or more but less than 100% by mass, or even 0.01% by mass or more but less than 50% by mass, relative to the mass of the seaweed powder. Iodine powder itself tends to reduce periodontal bacteria in a short period of time (e.g., within 1 minute), and can be added as an auxiliary agent. Zinc powder can also function as an auxiliary agent even in a small amount. The amount of zinc powder added can be, for example, 0.1% by mass or more but less than 40% by mass, relative to the mass of the seaweed powder.

[0072] The additive may further include one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder.Further, the additive may include laminaran powder, ascophyllum nodosum powder, ascorafen powder, β-carotene powder, and laver micron powder.

[0073] (Periodontal disease-improving dentifrice) A periodontal disease-improving dentifrice is a toothpaste or liquid dentifrice in which a periodontal disease improving agent is blended as an active ingredient. An appropriate amount of the periodontal disease improving agent is blended so that the periodontal disease improving agent is distributed throughout the oral cavity when brushing with the periodontal disease-improving dentifrice. As shown in Figure 6(c), the periodontal disease-improving dentifrice is applied to a toothbrush to a depth of about 1 cm to 3 cm when brushing teeth.

[0074] (Periodontal disease-improving mouthwash) A periodontal disease-improving mouthwash is a mouthwash solution containing a periodontal disease improving agent as an active ingredient. An appropriate amount of the periodontal disease improving agent is blended so that the periodontal disease improving agent is distributed throughout the oral cavity by rinsing with the periodontal disease-improving mouthwash.

[0075] (Periodontal disease improving gum) The periodontal disease improving gum is a gum containing a periodontal disease improving agent as an active ingredient. The periodontal disease improving agent is blended in an appropriate amount so that the periodontal disease improving agent is distributed throughout the oral cavity by chewing the periodontal disease improving gum.

[0076] (Method for Improving Periodontal Disease) The method for improving periodontal disease includes a step of spreading a periodontal disease improving agent throughout the oral cavity, and a step of repeating the step of spreading the agent throughout the oral cavity for a certain period of time.

[0077] The process of spreading the periodontal disease improving agent throughout the oral cavity can be carried out, for example, by applying an appropriate amount of the periodontal disease improving agent to a toothbrush and brushing the teeth, as shown in Figure 6(a). As shown in Figure 6(b), it is also possible to spread the periodontal disease improving agent throughout the oral cavity by applying an appropriate amount of toothpaste to a toothbrush and then brushing the teeth. As shown in Figure 6(c), it is also possible to spread the periodontal disease improving agent throughout the oral cavity by applying an appropriate amount of toothpaste containing the periodontal disease improving agent to a toothbrush and brushing the teeth. Alternatively, it is also possible to add an appropriate amount of the periodontal disease improving agent to a mouthwash and then rinsing the mouth to spread the agent throughout the oral cavity. It is also possible to add an appropriate amount of the periodontal disease improving agent to gum and then chew the gum to spread the agent throughout the oral cavity. The amount of periodontal disease improving agent used is adjusted appropriately depending on the tooth brushing time and frequency, mouth rinsing time and frequency, gum chewing time and frequency, etc., but it is desirable to use 0.01 g or more per day to distribute throughout the oral cavity, and more desirable to use 0.1 g or more.

[0078] An ultrasonic toothbrush can be used in the process of spreading the periodontal disease improving agent throughout the oral cavity. Ultrasound refers to sound waves of 20 kHz or higher, and ultrasonic toothbrushes are generally designed to operate at frequencies of approximately 1.6 MHz to 2.0 MHz. It is more preferable to apply ultrasonic vibrations to the ultrasonic toothbrush. Examples of ultrasonic vibrations include those at approximately 16,000 strokes / minute to 20,000 strokes / minute.

[0079] (Evaluation Test 1) An experiment was conducted to confirm the reduction of bacteria associated with periodontal disease in the human oral cavity using the oral bacteria-reducing agent.

[0080] Sample A and Sample B were prepared as oral fungus-reducing agents. Sample A: Kelp powder made by drying and crushing kelp from Hokkaido. Sample B: Sample A mixed with zinc powder (Sample A: zinc powder = 10:4 to 10:10).

[0081] Three subjects, Subject A (male in his 40s), Subject B (male in his 60s), and Subject C (male in his 30s), participated in Evaluation Test 1. At the start of the evaluation test (day 0), gingival crevicular fluid was collected with a paper tip from periodontal pockets in the oral cavity after pretreatment according to the standard oral bacterial PCR testing method. DNA was extracted and PCR reactions were performed to detect the total number of oral bacteria and the number of target periodontal bacteria. The target periodontal bacteria were Porphyromonas gingivalis (P. g), Treponema denticola (T. d), Tannerella forsythia (T. f), Aggregatibacter actinomycetemcomitans (A. a), and Fusobacterium nucleatum (F. n).

[0082] Next, almost every day after meals, the subjects brushed their teeth with toothpaste and Sample A on a toothbrush, as shown in Figure 6(b). Brushing time was about 15 seconds to 5 minutes.

[0083] After 308 days for subject A, 74 days for subject B, and 537 days for subject C, gingival crevicular fluid was collected with a paper tip from periodontal pockets in the oral cavity after the specified pretreatment, and DNA was extracted and PCR reactions were performed to detect the total number of bacteria in the oral cavity and the number of target periodontal disease bacteria.

[0084] After that, subjects A and B brushed their teeth after meals every day, placing toothpaste and sample B on their toothbrushes as shown in Figure 6(b). Brushing time ranged from 15 seconds to 5 minutes.

[0085] Gingival crevicular fluid was collected with a paper tip from periodontal pockets in the oral cavity after the prescribed pretreatment for Subject A 633 days after the start of the evaluation test (325 days as the application period of Sample B), and for Subject B 606 days after the start of the evaluation test (532 days as the application period of Sample B), and DNA was extracted and PCR reactions were performed to detect the total number of bacteria in the oral cavity and the number of target periodontal disease bacteria.

[0086] The results of evaluation test 1 are shown in Table 1. For Porphyromonas gingivalis (P. g), Treponema denticola (T. d), and Tannerella forsythia (T. f), the ratio of the number of target periodontal disease bacteria to the total number of bacteria in the oral cavity was calculated. The change in this ratio over time is shown in Figure 1. For Aggregatibacter actinomycetemcomitans (A. a) and Fusobacterium nucleatum (F. n), the copy numbers are shown directly in Table 1. In evaluation test 1, the detection limit was 10 copies.

[0087]

[0088] It was found that by repeatedly spreading Sample A (kelp powder) throughout the oral cavity, the number of target periodontal disease bacteria was reduced in all subjects. In particular, it was found that Porphyromonas gingivalis (P. g), Treponema denticola (T. d), and Tannerella forsythia (T. f) showed a significant reduction effect, with all subjects seeing numbers below the detection limit. Furthermore, since the total number of bacteria in the oral cavity is 100,000 to 600,000 copies, the fact that these periodontal disease bacteria were reduced to below the detection limit of 10 copies is truly an astonishing effect.

[0089] By repeatedly spreading sample B (kelp powder + zinc powder) throughout the oral cavity, it was found that the number of target periodontal disease bacteria was reduced in subjects A and B, or that there was no increase in the number of reduced periodontal disease bacteria.

[0090] (Evaluation Test 2) The oral fungus-reducing agent and its evaluation sample were used to evaluate the effect on Porphyromonas gingivalis (P. g bacteria).

[0091] The following samples 1 to 28 were prepared as evaluation samples. Sample 1: 2% aqueous solution of kelp powder (particle size 5 μm) Samples 2 and 3: 5% aqueous solution of kelp powder (particle size 5 μm) Sample 4: 5% aqueous solution of kelp powder (particle size unknown) Samples 5 and 6: 5% aqueous solution of dry-heat sterilized kelp powder Sample 7: 2% aqueous solution of kelp powder (particle size 5 μm) + 2% aqueous solution of iodine powder Sample 8: 2% aqueous solution of kelp powder (particle size 5 μm) + 10% aqueous solution of iodine powder Sample 9: 0.1% aqueous solution of iodine powder Sample 10: 0.2% aqueous solution of iodine powder Sample 11: 2% aqueous solution of iodine powder Sample 12: 5% aqueous solution of iodine powder Sample 13: 10% aqueous solution of iodine powder Sample 14: 5% aqueous solution of dry-heat sterilized kelp powder + 2% aqueous solution of zinc powder Sample 15: 2% aqueous solution of zinc powder Sample 16: 5% aqueous solution of zinc powder Sample 17: Aqueous solution of 2% kelp powder (particle size 5 μm) + 2% phloroglucinol powder. Sample 18: Aqueous solution of 2% kelp powder (particle size 5 μm) + 10% phloroglucinol powder. Sample 19: Aqueous solution of 2% phloroglucinol powder. Sample 20: Aqueous solution of 10% phloroglucinol powder. Sample 21: Aqueous solution of 2% kelp powder (particle size 5 μm) + 2% black grape skin powder. Sample 22: Aqueous solution of 2% kelp powder (particle size 5 μm) + 10% black grape skin powder. Sample 23: Aqueous solution of 10% laminaran powder. Sample 24: Aqueous solution of 2% Ascophyllum nodosum powder. Sample 25: Aqueous solution of 10% Ascophyllum nodosum powder. Sample 26: Aqueous solution of 5% ascorafine powder. Sample 27: Aqueous solution of 5% citric acid powder. Sample 28: Aqueous solution of 0.2% beta-carotene powder and 2% zinc powder. Each sample was dissolved in boiling water. Since the solubility of kelp powder in water is approximately 5%, some samples showed slight variations in solubility. Furthermore, since different lots and producers of kelp powder were used for some samples, some samples were evaluated multiple times to anticipate variations in the data.

[0092] For example, it was confirmed that kelp powder contains 304,000 μg / 100 g of iodine and 0.80 mg / 100 g of zinc. Also, the pH of sample 1 (aqueous solution of 2% kelp powder) was 6.1, the pH of sample 2 (aqueous solution of 5% kelp powder) was 6.0, and the pH of sample 10 (aqueous solution of 0.2% iodine powder) was 4.6. For reference, the pH of 5% Isodine® mouthwash was 3.1, and the pH of 10% Isodine® mouthwash was 2.7.

[0093] Porphyromonas gingivalis (P. g bacteria) was used as the test bacterium. A required amount was dissolved and pre-cultured on a blood agar medium under anaerobic conditions at 35°C for 5 days. The solution was then diluted with sterilized purified water to a predetermined concentration (for example, about 10 6 cfu / mL) to prepare the test bacterial solution. 0.1 mL of the test bacterial solution was added to 10 mL of sterile physiological saline for the control sample, and the sensitization times were 0, 30 seconds, 60 seconds, 300 seconds, and 7 hours. For evaluation samples 1 to 28, 0.1 mL of the test bacterial solution was added to 10 mL of each sample, and the sensitization times were 0, 30 seconds, 60 seconds, 300 seconds, and 7 hours. However, some sensitization times were omitted depending on the sample. For samples 9, 12, 14, and 28, the addition ratio was set to reduce the number of test bacteria at the start of the evaluation. The test method was based on JIS Z 2801 (Antibacterial processed products, antibacterial testing method, bactericidal effect) and the carbolic acid coefficient method.

[0094] The number of bacteria was measured at the start of the evaluation test and after a predetermined time had passed at room temperature. The results are shown in Table 2. Figure 2 also shows the time course of the reduction in the number of bacteria in Samples 1 to 6, which contained only kelp powder. The detection limit for the number of bacteria in this evaluation test was 10 copies, and no reduction in the number of bacteria was observed in the control sample.

[0095]

[0096] Table 2 and Figure 2 show that even for samples 1 to 6 containing only kelp powder, the bacterial count at the start of the test was reduced to 0.08% to 15% after 7 hours, although there was some variability. Furthermore, even for samples containing iodine powder, zinc powder, phloroglucinol powder, laminaran powder, ascophyllum nodosum powder, ascorafine powder, or citric acid powder alone, the bacterial count at the start of the test was reduced to 0.00% to 8% after 7 hours, assuming a 100% bacterial count at the start of the test. Furthermore, even when kelp powder was mixed with an auxiliary agent, the bacterial count at the start of the test was reduced to 0.00% to 4.2% after 7 hours, assuming a 100% bacterial count. Even considering the variability in the bacterial count reduction effect of kelp powder, a natural product, the fungal reduction effect of the addition of auxiliary agents (including black grape skin powder in addition to the above auxiliary agents) was confirmed.

[0097] (Evaluation Test 3) The oral fungus-reducing agent and its evaluation sample were used to evaluate the effect on Fusobacterium nucleatum (F.n bacteria).

[0098] The following samples 29 to 41 were prepared as evaluation samples. Sample 29: 5% aqueous solution of kelp powder Sample 30: 5% aqueous solution of dry-heat-sterilized kelp powder Sample 31: 5% aqueous solution of kelp powder and 1% iodine powder Sample 32: 5% aqueous solution of dry-heat-sterilized kelp powder and 1% iodine powder Sample 33: 0.1% aqueous solution of iodine powder Sample 34: 0.2% aqueous solution of iodine powder Sample 35: 5% aqueous solution of iodine powder Sample 36: 5% aqueous solution of dry-heat-sterilized kelp powder and 2% zinc powder Sample 37: 2% aqueous solution of zinc powder Sample 38: 5% aqueous solution of zinc powder Sample 39: 5% aqueous solution of laver micron powder Sample 40: 5% aqueous solution of ascorafine powder Sample 41: 5% aqueous solution of citric acid powder Each sample was dissolved in boiling water. Furthermore, since the solubility of kelp powder in water is approximately 5%, slight variations in solubility were observed depending on the sample. Also, since different lots and producers of kelp powder were used for some samples, some samples were evaluated multiple times in anticipation of variations in the data.

[0099] As an example, it was confirmed that kelp powder contains 304,000 μg / 100 g of iodine and 0.80 mg / 100 g of zinc.

[0100] The test bacteria used were Fusobacterium nucleatum (F.n bacteria). The required amount was dissolved and pre-cultured on blood agar medium under anaerobic conditions at 35°C for 5 days. The test bacteria solution was adjusted to a predetermined concentration with sterile purified water to prepare a test bacteria solution. For example, 0.1 mL of the test bacteria solution was added to 10 mL of sterile physiological saline for the control sample, and the sensitization times were set to 0, 60, 300, or 7 hours. The test bacteria solution was appropriately adjusted to obtain the desired number of bacteria. For evaluation samples 29 to 41, for example, 0.1 mL of the test bacteria solution was added to 10 mL of each sample, and the sensitization times were set to 0, 60, 300, or 7 hours. However, some sensitization times were omitted depending on the sample. The number of bacteria at the start of the test was also appropriately adjusted. The test method was based on JIS Z 2801 (Antibacterial Products, Antibacterial Testing Method, Bactericidal Effect) and the phenolic coefficient method.

[0101] The number of bacteria was measured at the start of the evaluation test and after a predetermined time had passed at room temperature. The results are shown in Table 3. The time course of the reduction in the number of bacteria in Samples 29 and 30, which contained only kelp powder, is shown in Figure 3. The detection limit for the number of bacteria in this evaluation test was 100 copies, and no reduction in the number of bacteria was observed in the control sample.

[0102]

[0103] Table 3 and Figure 3 show that even in samples 29 and 30 containing only kelp powder, the bacterial count at the start of the test was reduced to 0.34% or less (below the detection limit) after 7 hours, assuming 100% bacterial count at the start of the test. Furthermore, in samples containing only iodine powder, zinc powder, seaweed micron powder, ascorbyl powder, or citric acid powder as auxiliary agents, the bacterial count at the start of the test was reduced to 8% or less, assuming 100% bacterial count at the start of the test. Furthermore, even when auxiliary agents were added to kelp powder, the bacterial count at the start of the test was reduced to below the detection limit after 7 hours, assuming 100% bacterial count. This confirms the effectiveness of the auxiliary agents in reducing bacterial counts, even considering the variability in the bacterial count reduction effect of kelp powder, a natural product.

[0104] (Evaluation Test 4) The oral fungus-reducing agent and its evaluation sample were used to evaluate the effect on Tannerella forsythia (T. f. fungus).

[0105] The following samples 42 to 45 were prepared as evaluation samples. Sample 42: 5% dry-heat sterilized kelp powder + 2% zinc powder aqueous solution Sample 43: 0.1% iodine powder aqueous solution Sample 44: 0.2% iodine powder aqueous solution Sample 45: 5% iodine powder aqueous solution Each sample was dissolved in boiling water. Furthermore, since the solubility of kelp powder in water is approximately 5%, the results may vary depending on the sample.

[0106] Tannerella forsythia (T. f.) was used as the test bacterium. The required amount was dissolved and pre-cultured in NAM medium under anaerobic conditions at 35°C for 5 days. The test bacterium was then adjusted to a predetermined concentration with sterile purified water to prepare a test bacterium solution. The test bacterium solution was added to 10 mL of sterile physiological saline for the control sample, and the sensitization times were 0, 60, 300, or 7 hours. The test bacterium solution was adjusted appropriately to achieve the desired number of bacteria. For evaluation samples 42 to 45, the test bacterium solution was added to 10 mL of each sample, and the sensitization times were 0, 60, 300, or 7 hours. However, some sensitization times were omitted depending on the sample. The number of bacteria at the start of the test was adjusted appropriately. The test method was based on JIS Z 2801 (Antibacterial Products, Antibacterial Testing Methods, Bactericidal Effect) and the phenolic coefficient method.

[0107] The number of bacteria was measured at the start of the evaluation test and after a predetermined time had passed at room temperature. The results are shown in Table 4. Figure 4 shows the change in the number of bacteria over time for sample 42, which was a mixture of kelp powder and zinc powder as an auxiliary agent. The detection limit for the number of bacteria in this evaluation test was 100 copies, and no reduction in the number of bacteria was observed in the control sample.

[0108]

[0109] Table 4 and Figure 4 show that in sample 42, in which kelp powder was mixed with zinc powder as an auxiliary agent, the bacterial count at the start of the test was reduced to 25.8% after 7 hours, assuming 100% bacterial count at the start of the test. Furthermore, in the sample containing only iodine powder as an auxiliary agent, the bacterial count at the start of the test was reduced to below the detection limit after 7 hours, assuming 100% bacterial count at the start of the test. In the above-mentioned evaluation test 1, a significant reduction in Tannerella forsythia (T. f. bacteria) was confirmed even with kelp powder alone. In this evaluation test 4, evaluation of samples containing only kelp powder has not yet been initiated, but it is believed that a similar reduction effect would be observed.

[0110] (Evaluation Test 5) The oral fungus-reducing agent and its evaluation sample were used to evaluate the effect on Treponema denticola (T. d bacteria).

[0111] The following samples 46 to 49 were prepared for evaluation. Sample 46: 5% dry-heat sterilized kelp powder + 2% zinc powder aqueous solution Sample 47: 0.1% iodine powder aqueous solution Sample 48: 0.2% iodine powder aqueous solution Sample 49: 5% iodine powder aqueous solution Each sample was dissolved in boiling water. Furthermore, since the solubility of kelp powder in water is approximately 5%, the results may vary depending on the sample.

[0112] The test bacteria used were Treponema denticola (T. d bacteria). The required amount was dissolved in boiling water (purified water), pre-cultured on blood agar medium under anaerobic conditions at 35°C for 5 days, and then adjusted to a predetermined concentration with sterile purified water to prepare a test bacterial solution. The test bacterial solution was added to 10 mL of sterile physiological saline for the control sample, and the sensitization times were 0, 60, 300, or 7 hours. The test bacterial solution was adjusted appropriately to achieve the desired bacterial count. For evaluation samples 46 to 49, the test bacterial solution was added to 10 mL of each sample, and the sensitization times were 0, 60, 300, or 7 hours. However, some sensitization times were omitted depending on the sample. The bacterial count at the start of the test was adjusted appropriately. The test method was based on JIS Z 2801 (Antibacterial Products, Antibacterial Testing Method, Bactericidal Effect) and the carbolic acid coefficient method.

[0113] The number of bacteria was measured at the start of the evaluation test and after a predetermined time had elapsed at room temperature. The results are shown in Table 5 and Figure 5. The detection limit for the number of bacteria in this evaluation test was 100 copies, and no reduction in the number of bacteria was observed in the control sample.

[0114]

[0115] Table 5 and Figure 5 show that in sample 46, in which kelp powder was mixed with zinc powder as an auxiliary agent, the bacterial count at the start of the test was reduced to 22.7% after 7 hours, assuming 100% bacterial count at the start of the test. Furthermore, in the sample containing only iodine powder as an auxiliary agent, the bacterial count at the start of the test was reduced to below the detection limit after 7 hours, assuming 100% bacterial count at the start of the test. In the above-mentioned evaluation test 1, a significant reduction in Treponema denticola (T. d bacteria) was confirmed even with kelp powder alone. In this evaluation test 5, evaluation of samples containing only kelp powder has not yet been initiated, but it is believed that a similar reduction effect would be observed.

[0116] (Evaluation Test 6) Using the oral fungus-reducing agent and its evaluation sample, the effect of an ultrasonic toothbrush on Porphyromonas gingivalis (P. g bacteria) was evaluated.

[0117] The following samples 50 to 53 were prepared as evaluation samples. Sample 50: Kelp powder made by drying and crushing kelp from Hokkaido Sample 51: 5% kelp powder in physiological saline solution Sample 52: Control sample (no oral fungus-reducing agent, no ultrasound, no sonic vibration) Sample 53: Control sample (no oral fungus-reducing agent, with ultrasound and sonic vibration)

[0118] Porphyromonas gingivalis (P. g) was used as the test bacterium. A required amount of the test bacterium was dissolved and pre-cultured on a blood agar medium under anaerobic conditions at 35°C for 5 days or more, and then diluted with sterilized purified water for about 10 minutes. 8 The concentration was adjusted to CFU / mL to prepare a test bacteria stock solution. 10 mL of physiological saline was placed in a container (inner diameter 2 cm, height 4 cm), and 0.1 mL of the test bacteria stock solution was added to prepare a test bacteria solution.

[0119] For sample 50, the solution was evenly sprinkled on the brush part of a toothbrush moistened with saline, and the toothbrush was immersed in the test bacteria solution in the container. It was then subjected to ultrasound (frequency 1.6 MHz) and sonic vibration (16,000 strokes / min) for 60 seconds, followed by a reaction for 300 seconds. The number of test bacteria remaining in the test bacteria solution was measured according to the microbiological testing method. For sample 51, a 5% saline solution of kelp powder was applied to a toothbrush, which was then immersed in the test bacteria solution in the container. It was subjected to ultrasound (frequency 1.6 MHz) and sonic vibration (16,000 strokes / min) for 60 seconds, followed by a reaction for 300 seconds. The number of test bacteria remaining in the test bacteria solution was measured according to the microbiological testing method. For the control sample, sample 52, a toothbrush was immersed in the test bacteria solution in the container, and the number of test bacteria remaining in the test bacteria solution was measured according to the microbiological testing method after 300 seconds. For the control sample of sample 53, a toothbrush was immersed in the test bacteria solution in the container and activated with ultrasound (frequency 1.6 MHz) and sonic vibration (16,000 strokes / min) for 60 seconds, and then 300 seconds later, the number of test bacteria remaining in the test bacteria solution was measured according to the microorganism testing method.

[0120] The number of bacteria was measured at the start of the evaluation test and after a predetermined time had passed at room temperature. The results are shown in Table 6.

[0121]

[0122] Table 6 shows that when kelp powder was sprinkled or applied to a toothbrush and ultrasonic and sonic vibrations were applied to the toothbrush, the concentration of Porphyromonas gingivalis (P. g bacteria) was reduced to 80.0% after 300 seconds, assuming the bacterial concentration at the start of the test was 100.0%. In the above-mentioned Evaluation Test 1, a significant reduction in Porphyromonas gingivalis (P. g bacteria) was confirmed even by manual toothbrushing, but in this Evaluation Test 6, it is believed that applying ultrasonic and sonic vibrations to the toothbrush will have an even greater effect in reducing the bacterial concentration.

[0123] (Evaluation Test 7) ​​An experiment was conducted using the periodontal disease improving agent to confirm the reduction in bleeding rate associated with periodontal disease in the human oral cavity.

[0124] Sample A was prepared as a periodontal disease improving agent. Sample A: Kelp powder made by drying and pulverizing kelp from Hokkaido.

[0125] Subject D (male, in his 60s) cooperated in evaluation test 7. At the start of the evaluation test (when the number of days elapsed was 0), a probing test was performed to check the presence or absence of bleeding and the depth of the gum pocket (periodontal pocket).

[0126] Next, almost every day after meals, the subjects brushed their teeth with toothpaste and Sample A on a toothbrush, as shown in Figure 6(b). Brushing time was about 15 seconds to 5 minutes.

[0127] For subject D, a probing test was performed 66 days, 101 days, and 130 days after the start of the evaluation test to check the presence or absence of bleeding and the depth of the periodontal pockets.

[0128] The probing test, also known as a gum pocket test, involves inserting a graduated probe into the gum pocket to measure the depth from the gum entrance to the bottom of the pocket while simultaneously checking for the presence or absence of bleeding. The test is performed at six locations per tooth. The depth of a gum pocket is approximately 1-2 mm in healthy gums, and a depth of 4 mm or more is diagnosed as periodontal disease. The presence or absence of bleeding is calculated as the percentage of bleeding locations out of the total number of locations tested (for 32 teeth, 32 x 6 = 192 locations). A bleeding rate of 20% or less is considered desirable.

[0129] The results of Evaluation Test 7 are shown in Table 7.

[0130]

[0131] The results in Table 7 show that the bleeding rate improved from 2.60% at the start of the evaluation test to 0% (no bleeding). It was also found that the number of gum pockets with a depth of 4 mm or more improved from seven at the start of the evaluation test to no bleeding at all test sites. These results also demonstrate that the periodontal disease improving agent of the present invention not only reduces oral bacteria but also significantly improves periodontal disease itself.

[0132] (Evaluation Test 8) A confirmation test was carried out on the pH of seaweeds within the scope of the present invention. The pH was measured using the glass electrode method. The following samples 54 to 62 were prepared as evaluation samples. Sample 54: Heat-treated 5% kelp powder aqueous solution Sample 55: Heat-treated 2% kelp powder aqueous solution Sample 56: Root kelp powder aqueous solution heated and filtered at 80°C for 5 minutes Sample 57: Root kelp powder precipitate precipitated in the aqueous solution after heating and filtering at 80°C for 5 minutes Sample 58: Root kelp powder residue remaining on the filter paper after heating and filtering at 80°C for 5 minutes Sample 59: Root kelp powder Sample 60: Kelp powder precipitate after boiling Sample 61: Kelp powder aqueous solution after boiling Sample 62: Habomai kelp powder

[0133] Table 8 shows the results of Evaluation Test 8.

[0134]

[0135] From Table 8, it was confirmed that the pH of Samples 54 to 62, which are exemplified as seaweeds of the present invention, was in the range of 5 or more and 8 or less.

[0136] The present invention may comprise the following aspects: [Item 1] An oral fungus-reducing agent containing seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of the seaweed, and contains 0.01 mass% or more of iodine, and the oral fungus is one or more species selected from the group consisting of periodontal disease bacteria Porphyromonas gingivalis (P. g), Treponema denticola (T. d), Tannerella forsythia (T. f), Aggregatibacter actinomycetemcomitans (A. a), and Fusobacterium nucleatum (F. n), and the oral fungus-reducing agent reduces the number of oral fungi by spreading the oral fungus-reducing agent throughout the oral cavity. [Item 2] The oral fungus-reducing agent according to Item 1, wherein the oral fungus is Porphyromonas gingivalis (P. g), and the ratio of the number of Porphyromonas gingivalis (P. g) to the total number of bacteria in the oral cavity is reduced to 0.49% or less. [Item 3] The oral fungus-reducing agent according to Item 1, wherein the oral fungus is Treponema denticola (T. d), and the ratio of the number of Treponema denticola (T. d) to the total number of bacteria in the oral cavity is reduced to 0.49% or less. [Item 4] The oral fungus-reducing agent according to Item 1, wherein the oral fungus is Tannerella forsythia (T. f), and the ratio of the number of Tannerella forsythia (T. f) to the total number of bacteria in the oral cavity is reduced to 0.49% or less. [Item 5] The oral fungus-reducing agent according to Item 1, wherein the oral fungi are all selected from the group consisting of Porphyromonas gingivalis (P. g), Treponema denticola (T. d), and Tannerella forsythia (T. f), and the agent simultaneously reduces the ratio of Porphyromonas gingivalis (P. g) to 0.49% or less, the ratio of Treponema denticola (T. d) to 0.49% or less, and the ratio of Tannerella forsythia (T. f) to 0.49% or less, relative to the total number of bacteria in the oral cavity. [Item 6] The oral fungus-reducing agent according to any one of Items 1 to 5, wherein the seaweed powder has a pH of 5 or more and 8 or less. [Item 7] The oral fungus-reducing agent according to Item 1, further comprising iodine powder and / or zinc powder.[Item 8] The oral fungus-reducing agent according to Item 8, wherein the iodine powder is present in an amount of 0.01% by mass or more and less than 100% by mass relative to the mass of the seaweed powder. [Item 9] The oral fungus-reducing agent according to Item 1, further comprising one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder. [Item 10] An oral fungus-reducing dentifrice, in which the oral fungus-reducing agent according to Items 1 to 9 is incorporated as an active ingredient into toothpaste or liquid toothpaste. [Item 11] An oral fungus-reducing mouthwash, in which the oral fungus-reducing agent according to Items 1 to 9 is incorporated as an active ingredient into a mouthwash solution. [Item 12] An oral fungus-reducing gum, in which the oral fungus-reducing agent according to Items 1 to 9 is incorporated as an active ingredient into gum. [Item 13] A method for reducing oral fungi, comprising the steps of: distributing the oral fungi-reducing agent according to Item 1 throughout the oral cavity; and repeating the step of distributing the agent throughout the oral cavity for a certain period of time. [Item 14] The method for reducing oral fungi according to Item 13, wherein the step of distributing the agent throughout the oral cavity comprises distributing 0.01 g or more of the oral fungi-reducing agent throughout the oral cavity per day. [Item 15] The method for reducing oral fungi according to Item 13 or 14, wherein the step of distributing the agent throughout the oral cavity comprises using an ultrasonic toothbrush. [Item 16] A periodontal disease improving agent comprising seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of the seaweed, and contains 0.01 mass% or more of iodine, wherein the periodontal disease improving agent improves periodontal disease by distributing the oral fungi-reducing agent throughout the oral cavity. [Item 17] A periodontal disease-improving dentifrice, in which the periodontal disease improving agent according to Item 16 is incorporated as an active ingredient into toothpaste or liquid toothpaste. [Item 18] A periodontal disease-improving mouthwash, in which the periodontal disease improving agent according to Item 16 is incorporated as an active ingredient into a mouthwash solution. [Item 19] A periodontal disease-improving gum, in which the periodontal disease improving agent according to Item 16 is incorporated as an active ingredient into a gum. [Item 20] A method for improving periodontal disease, comprising the steps of: spreading the periodontal disease improving agent according to Item 16 throughout the oral cavity; and repeating the step of spreading the agent throughout the oral cavity for a certain period of time.[Item 21] The method for improving periodontal disease according to Item 20, wherein the step of spreading the periodontal disease improving agent throughout the oral cavity comprises spreading 0.01 g or more of the periodontal disease improving agent throughout the oral cavity per day. [Item 22] The method for reducing oral fungi according to Item 20 or 21, wherein the step of spreading the agent throughout the oral cavity comprises using an ultrasonic toothbrush.

Claims

1. An oral fungus-reducing agent comprising seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of the seaweed, and contains 0.01 mass% or more of iodine, and the oral fungus is one or more selected from the group consisting of periodontal disease bacteria Porphyromonas gingivalis (P. g), Treponema denticola (T. d), Tannerella forsythia (T. f), Aggregatibacter actinomycetemcomitans (A. a), and Fusobacterium nucleatum (F. n), and the oral fungus-reducing agent reduces the number of oral fungi by spreading the oral fungus-reducing agent throughout the oral cavity.

2. The oral fungus-reducing agent according to claim 1, wherein the oral fungus is Porphyromonas gingivalis (P. g), and the ratio of the number of Porphyromonas gingivalis (P. g) bacteria to the total number of bacteria in the oral cavity is reduced to 0.49% or less.

3. The oral fungus-reducing agent according to claim 1, wherein the oral fungus is Treponema denticola (T. d), and the ratio of the number of Treponema denticola (T. d) bacteria to the total number of bacteria in the oral cavity is reduced to 0.49% or less.

4. An oral fungus-reducing agent as described in claim 1, wherein the oral fungus is Tannerella forsythia (T. f.), and the ratio of the number of Tannerella forsythia (T. f.) bacteria to the total number of bacteria in the oral cavity is reduced to 0.49% or less.

5. The oral fungus-reducing agent according to claim 1, wherein the oral fungi are all selected from the group consisting of Porphyromonas gingivalis (P. g), Treponema denticola (T. d), and Tannerella forsythia (T. f), and the agent simultaneously reduces the ratio of Porphyromonas gingivalis (P. g) bacteria to 0.49% or less, reduces the ratio of Treponema denticola (T. d) bacteria to 0.49% or less, and reduces the ratio of Tannerella forsythia (T. f) bacteria to 0.49% or less relative to the total number of bacteria in the oral cavity.

6. The oral fungus-reducing agent according to claim 1, wherein the seaweed powder has a pH of 5 or more and 8 or less.

7. The oral fungus-reducing agent according to claim 1, further comprising iodine powder and / or zinc powder.

8. The oral fungus-reducing agent according to claim 7, wherein the iodine powder is present in an amount of 0.01% by mass or more and less than 100% by mass relative to the mass of the seaweed powder.

9. The oral fungus-reducing agent according to claim 1, further comprising one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder.

10. A toothpaste or liquid toothpaste for reducing oral bacteria, comprising the oral bacteria-reducing agent according to claim 1 as an active ingredient.

11. A mouthwash for reducing oral fungi, comprising the oral fungi-reducing agent according to claim 1 as an active ingredient.

12. An oral fungus-reducing gum comprising the oral fungus-reducing agent according to claim 1 as an active ingredient.

13. A method for reducing oral fungi, comprising: a step of distributing the oral fungi-reducing agent according to claim 1 throughout the oral cavity; and a step of repeating the step of distributing the agent throughout the oral cavity for a certain period of time.

14. A method for reducing oral fungi as described in claim 13, wherein the step of distributing the oral fungi-reducing agent in the oral cavity includes a step of distributing 0.01 g or more of the oral fungi-reducing agent in the oral cavity per day.

15. A method for reducing oral fungi as described in claim 13 or 14, wherein the step of infesting the oral cavity includes using an ultrasonic toothbrush.

16. A periodontal disease improving agent comprising seaweed powder as an active ingredient, the seaweed powder being a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of the seaweed, and containing 0.01 mass% or more of iodine, the periodontal disease improving agent improving periodontal disease by distributing the oral bacteria-reducing agent throughout the oral cavity.

17. A toothpaste or liquid toothpaste for improving periodontal disease, comprising the periodontal disease improving agent according to claim 16 as an active ingredient.

18. A mouthwash for improving periodontal disease, comprising the periodontal disease improving agent according to claim 16 as an active ingredient.

19. A gum for improving periodontal disease, comprising the agent for improving periodontal disease according to claim 16 as an active ingredient.

20. A method for improving periodontal disease, comprising: a step of distributing the periodontal disease improving agent according to claim 16 throughout the oral cavity; and a step of repeating the step of distributing the agent throughout the oral cavity for a certain period of time.

21. A method for improving periodontal disease according to claim 20, wherein the step of spreading the agent in the oral cavity includes a step of spreading 0.01 g or more of the periodontal disease improving agent in the oral cavity per day.

22. A method for reducing oral fungi as described in claim 20 or 21, wherein the step of infesting the oral cavity includes using an ultrasonic toothbrush.

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