Acne removal and repair composition containing recombinant collagen and use thereof

Abstract

The present application relates to an acne removal and repair composition containing a recombinant collagen and use thereof. The acne removal and repair composition comprises azelaic acid, nisin, acetylcysteine, and the recombinant collagen. The present application inventively uses the four components in combination. The azelaic acid, the nisin, and the acetylcysteine have a potential mutually promoting effect in inhibiting Cutibacterium acnes and resisting inflammation, thus having a synergistic effect in terms of inhibiting and relieving acne formation and repairing acne. The recombinant collagen can repair skin damaged by acne and inflammation and can provide sufficient nutritional ingredients for the damaged skin to help the skin achieve better repair. As the recombinant collagen can further promote the efficacy of the azelaic acid, the nisin, and the acetylcysteine in acne removal, the four components work together through different mechanisms and from different perspectives to achieve the effect of acne removal and repair. In addition, the composition can well balance excellent acne removal and repair effects with exceptional mildness.

Description

Acne-removing and repairing composition containing recombinant collagen and its application Technical Field

[0001] The present application belongs to the technical field of acne-removing products, and relates to an acne-removing and repairing composition containing recombinant collagen and its application. Background Art

[0002] Acne, also known as pimples, blackheads, or pimples, is a common and recurring skin condition. Its root cause is a chronic skin disease that occurs in the sebaceous glands of the hair follicles. Excessive oil secretion leads to clogged pores. It often occurs in areas rich in sebaceous glands, such as the head, face, and neck, negatively impacting beauty.

[0003] Propionibacterium acnes is considered the primary cause of acne. This is caused by excessive oil secretion in the skin, which leads to excessive sebaceous cell proliferation. Oil is secreted into the sebaceous glands and spreads through the pores. When P. acnes comes into contact with oil, the triglycerides in the oil are broken down into glycerol and fatty acids. Glycerol provides nutrients for the bacteria, leading to bacterial proliferation, while free fatty acids cause keratinization of the skin pores. Hyperkeratinization leads to clogged pores. The bacterial proliferation and clogged pores lead to acne.

[0004] Common topical medications for acne treatment include tretinoin, benzoyl peroxide, and antibiotics. However, tretinoin can increase photosensitivity and cause redness; benzoyl peroxide can be irritating, causing redness and peeling in some patients; and antibiotics can promote bacterial resistance, so prolonged use is recommended.

[0005] The prior art also discloses many types of cosmetics with acne-removing effects. For example, CN107737033A discloses a composition for quickly removing acne and acne scars and its preparation method. The raw materials include the following components, in parts by weight: 1.2-1.8 parts of salicylic acid, 2-2.77 parts of arginine, 0.5-2 parts of Usnea barbata extract, 1-10 parts of glabridin liposomes, 0.03-0.1 parts of O-cymene-5-ol, 1-12 parts of other acne-removing ingredients, and 75-90 parts of other base materials. This product combines a specific amount of salicylic acid with arginine, compounding a specific amount of Usnea barbata extract and glabridin liposomes, supplemented with O-cymene-5-ol as a mild and effective preservative and bactericidal agent, and then adding other acne-removing ingredients. The product has good acne-removing effects. However, the raw material contains salicylic acid, which can irritate the skin and mucous membranes, may damage the skin barrier, and may cause allergic symptoms. It is not suitable for people with sensitive skin.

[0006] Currently, there are numerous acne-removing cosmetics on the market. While their production processes and formulas are largely similar, they offer varying degrees of protective and beautifying benefits for the skin. However, these results are suboptimal, and they lack the multifaceted anti-inflammatory, repair, and care properties for facial acne and pimples. Furthermore, these products typically lose their effectiveness within a short period of application, making it difficult for their active ingredients to remain active on the skin for extended periods, effectively eliminating acne and ensuring efficacy.

[0007] Therefore, it is very meaningful to develop a product that is gentle, safe and can effectively treat acne in the long term.

[0008] Summary of the Invention

[0009] The following is a summary of the subject matter described in detail herein. This summary is not intended to limit the scope of the claims.

[0010] In view of the shortcomings of the existing technology, the purpose of this application is to provide an acne-repairing composition containing recombinant collagen and its application.

[0011] To achieve this goal, this application adopts the following technical solutions:

[0012] In a first aspect, the present application provides an acne-repairing composition containing recombinant collagen, wherein the acne-repairing composition comprises azelaic acid, nisin, acetylcysteine ​​and recombinant collagen.

[0013] Azelaic acid has antibacterial and anti-inflammatory effects, regulates hair follicle keratinization, inhibits oil secretion, reduces pigmentation, and inhibits cell proliferation; nisin can interact with anionic lipids on bacterial cell membranes, thereby causing disturbances in the cell membrane and achieving the effect of inhibiting bacteria; acetylcysteine ​​can be used in cosmetics and skin care products as a skin conditioner, antioxidant, or protease inhibitor; recombinant collagen is collagen prepared using DNA recombination technology. Collagen can supplement the amino acids needed by the skin, repair damaged skin, and provide the skin with essential nutrients.

[0014] This application creatively combines four components: azelaic acid, nisin, acetylcysteine, and recombinant collagen. Azelaic acid, nisin, and acetylcysteine ​​have potential for mutually reinforcing effects in inhibiting Propionibacterium acnes and combating inflammation, thereby synergizing in suppressing and alleviating acne formation and repairing acne. Recombinant collagen can repair skin damaged by acne and inflammation, providing sufficient nutrients to help damaged skin repair itself, as it can further promote the acne-removing effects of azelaic acid, nisin, and acetylcysteine. These four components, working from different mechanisms and perspectives, work together to achieve acne-removing and repairing effects. This composition simultaneously balances excellent acne-removing and repairing efficacy with excellent mildness.

[0015] In one embodiment, the acne-removing and repairing composition comprises, by weight, 4-10 parts of azelaic acid, 0.1-1 parts of nisin, 0.5-5 parts of acetylcysteine, and 0.01-1 parts of recombinant collagen.

[0016] Based on the potential interactions between the above ingredients, when the above four ingredients are combined in a specific mass ratio, they have better effects in removing acne and repairing skin.

[0017] The mass fraction of the azelaic acid can be 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.; the mass fraction of the nisin can be 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, etc.; the mass fraction of the acetylcysteine ​​can be 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.; the mass fraction of the recombinant collagen can be 0.01 parts, 0.03 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.7 parts, 0.8 parts, 1 parts, etc. Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0018] In one embodiment, the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO: 1.

[0019] Compared with recombinant collagen from other sources, the present application also found that the recombinant collagen from the above specific sources has better effects in antibacterial, anti-inflammatory, acne removal and skin repair.

[0020] SEQ ID NO: 1:

[0021] In a second aspect, the present application provides a use of the acne-removing and repairing composition containing recombinant collagen according to the first aspect in the preparation of an acne-removing and repairing product.

[0022] In one embodiment, the amount of the acne-removing and repairing composition added to the acne-removing and repairing product is 1-20%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 20%, etc. Other specific values ​​within this numerical range can be selected and will not be repeated here.

[0023] In one embodiment, the product comprises a cosmetic or a pharmaceutical.

[0024] In a third aspect, the present application provides a gel with acne-removing and repairing effects, wherein the raw materials for preparing the gel include, by mass percentage: 1-20% of the acne-removing and repairing composition described in the first aspect, 0.5-5% of the gel, 0.2-2% of a solubilizer, 5-20% of a polyol, 0.1-1% of triethanolamine, and 50-90% of water.

[0025] The mass percentage of the acne-removing and repairing composition can be selected as 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 20%, etc.; the mass percentage of the gel can be selected as 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 3%, 4%, 5%, etc.; the mass percentage of the solubilizing agent can be selected as 0.2%, 0.3%, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, etc. The mass percentage of the polyol can be selected as 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 20%, etc.; the mass percentage of the triethanolamine can be selected as 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.7%, 0.8%, 0.9%, 1%, etc.; the mass percentage of the water can be selected as 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, etc.; other specific point values ​​within the above numerical range can be selected, which will not be repeated here.

[0026] In one embodiment, the gel comprises carbomer.

[0027] In one embodiment, the solubilizing agent comprises PPG-26-buteth-26 and / or PEG-40 hydrogenated castor oil.

[0028] In one embodiment, the polyol includes any one of propylene glycol, butylene glycol, or glycerol, or a combination of at least two thereof.

[0029] In one embodiment, the raw materials for preparing the gel further include, by mass percentage, any one of 1-10% of a plant-derived conditioning agent, 0.05-0.5% of an antioxidant, or 0.1-0.5% of a fragrance, or a combination of at least two of them.

[0030] The mass percentage of the plant-based conditioner can be selected as 1%, 2%, 3%, 4%, 5%, 6%, 8%, 10%, etc.; the mass percentage of the antioxidant can be selected as 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, etc.; the mass percentage of the fragrance can be selected as 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, etc.; other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0031] In one embodiment, the plant-derived conditioning agent includes a Sufanghua flower extract and / or a Kapok flower extract.

[0032] The present applicants have also creatively discovered that the addition of a Sufanghua extract and / or a Kapok flower extract, particularly a combination of a Sufanghua extract and a Kapok flower extract, to the above-mentioned product can further enhance the product's antibacterial, anti-inflammatory, and acne-removing effects. The Sufanghua extract and the Kapok flower extract also exhibit a synergistic effect in achieving the above-mentioned effects.

[0033] In a fourth aspect, the present application provides a method for preparing the gel having acne-removing and repairing effects according to the third aspect, the preparation method comprising the following steps:

[0034] The gel is mixed with part of the water at 25-45° C. to obtain solution A; the acne-removing and repairing composition, the solubilizer, the polyol and the remaining water are mixed at 30-50° C. to obtain solution B; solution A and solution B are mixed at 20-40° C., and triethanolamine and the remaining preparation raw materials are added to obtain the gel with acne-removing and repairing efficacy.

[0035] The specific point values ​​in the range of 25-45°C can be selected from 25°C, 28°C, 30°C, 32°C, 35°C, 38°C, 40°C, 42°C, 45°C, etc.; the specific point values ​​in the range of 30-50°C can be selected from 30°C, 33°C, 35°C, 38°C, 40°C, 42°C, 45°C, 48°C, 50°C, etc.; the specific point values ​​in the range of 20-40°C can be selected from 20°C, 22°C, 25°C, 28°C, 30°C, 32°C, 35°C, 38°C, 40°C, etc.; other specific point values ​​within the above numerical range can be selected, and they will not be listed here one by one.

[0036] Compared with the prior art, this application has the following beneficial effects:

[0037] This application creatively combines four components: azelaic acid, nisin, acetylcysteine, and recombinant collagen. Azelaic acid, nisin, and acetylcysteine ​​have potential for mutual promotion in inhibiting Propionibacterium acnes and fighting inflammation, thereby synergizing in suppressing and relieving acne formation and repairing acne. Recombinant collagen can repair skin damaged by acne and inflammation, providing sufficient nutrients to damaged skin to help it repair better, as it can further promote the acne-removing effects of azelaic acid, nisin, and acetylcysteine. The four components work together to achieve acne-removing and repairing effects from different mechanisms and perspectives. At the same time, the composition is able to strike a good balance between excellent acne-removing and repairing efficacy and excellent mildness.

[0038] Still other aspects will become apparent upon reading and understanding the detailed description. DETAILED DESCRIPTION

[0039] In order to further illustrate the technical means and effects adopted by this application, the technical solution of this application is further explained below in combination with the preferred embodiments of this application, but this application is not limited to the scope of the embodiments.

[0040] The mass fractions of each component in the composition products involved in the following content are calculated based on the actual active ingredient content in the commercially available raw materials.

[0041] The Sufanghua extract involved in the following content is derived from the products of Lanzhou Waterless Biotechnology Co., Ltd.; the kapok flower extract is derived from the products of Lanzhou Waterless Biotechnology Co., Ltd.; and the nisin is derived from the products of Guangzhou Tianjia Biotechnology Co., Ltd.

[0042] The recombinant collagen involved in the following content is the product prepared in the preparation example. The pET-28a(+) vector in the preparation example was purchased from Maclean; BL21(DE3) chemically competent cells were purchased from Merck with product number CMC0014-4X40UL.

[0043] Preparation Example

[0044] This preparation example provides a recombinant collagen freeze-dried powder, and the preparation method thereof is carried out according to the method disclosed in Preparation Example 1 in ZL 202310123476.3, as follows:

[0045] (1) Construction of recombinant plasmid: According to the nucleotide sequence shown in SEQ ID NO: 1, the pET-28a(+) vector suitable for the expression vector Escherichia coli host was selected and sent to a sequencing company for synthesis to obtain the recombinant collagen expression vector pET-28a-1.

[0046] (2) Construction of recombinant fermentation strain: The pET-28a-1 expression vector was transformed into Escherichia coli (BL21 (DE3)) competent cells by chemical transformation. Positive clones were screened in the transformed plate colonies by agarose gel electrophoresis. The screened positive clones were transferred to 2 mL LB medium and cultured at 37°C, 200 rpm for 12 h. The cultured bacterial solution was diluted 1-fold with 50% glycerol by volume, distributed into cryovials, and frozen at -80°C to obtain a recombinant collagen fermentation strain.

[0047] (3) Fermentation culture of recombinant fermentation strain: 10 μL of recombinant collagen fermentation liquid was spread on a solid LB plate, cultured in a 37°C incubator for 12 h, and then a single clone was selected and inoculated into 50 mL of LB liquid culture medium containing 50 μg / mL kanamycin. The culture was placed in a shaker at 37°C, 200 rpm for 14 h, and then inoculated into 200 mL of LB liquid culture medium containing 50 μg / mL kanamycin at a ratio of 1:100. The culture was cultured at 37°C until the OD value of the bacterial liquid was 0.6, and then IPTG was added to make the final concentration reach 1 mM. The culture was continued for 4 h, and then the bacteria were collected by centrifugation at 4°C, 4000 rpm for 15 min.

[0048] (4) Purification of recombinant collagen: The cells were ultrasonically disrupted and centrifuged, and the precipitate was washed with Triton X-100 washing solution, and the supernatant was removed by centrifugation again. 6 M guanidine hydrochloride was added to the precipitate, and the pH was adjusted to 8. After the precipitate was fully dissolved, the solution was passed through nickel affinity chromatography resin and eluted in sequence using 20 mM, 50 mM, 100 mM, 200 mM, and 500 mM concentration gradient imidazole solutions. The eluate was concentrated using a protein concentration column, and the concentrate was collected and dialyzed overnight (dialysis bag, RC membrane, 14 KD, flat width 44 mm (Sangong F600112)). The protein concentration was then detected using a BCA kit, and 0.5 mL of a 5% volume concentration mannitol aqueous solution was added to every 5 mg of protein and mixed with the mannitol aqueous solution. The solution was then pre-frozen at -40°C for 10 min using a freeze dryer, and finally freeze-dried at -35°C to obtain recombinant collagen freeze-dried powder.

[0049] Example 1

[0050] This embodiment provides an acne-removing and repairing composition, which is composed of the following components by weight: 5 parts of azelaic acid, 1 part of nisin, 3 parts of acetylcysteine, and 0.08 parts of recombinant collagen. The components can be physically mixed evenly according to the weight ratio.

[0051] Example 2

[0052] This embodiment provides an acne-removing and repairing composition, which is composed of the following components by weight: 4 parts of azelaic acid, 0.5 parts of nisin, 5 parts of acetylcysteine, and 0.05 parts of recombinant collagen. The components can be physically mixed evenly according to the weight ratio.

[0053] Example 3

[0054] This embodiment provides an acne-removing and repairing composition, which is composed of the following components by weight: 7 parts of azelaic acid, 0.2 parts of nisin, 2 parts of acetylcysteine, and 0.03 parts of recombinant collagen. The components can be physically mixed evenly according to the weight ratio.

[0055] Comparative Example 1

[0056] This comparative example provides a composition that differs from Example 1 only in that it does not contain azelaic acid. The reduced mass of azelaic acid is proportionally distributed to the mass of nisin and acetylcysteine, while the other components and their contents remain unchanged. The components are physically mixed uniformly according to the mass ratio.

[0057] Comparative Example 2

[0058] This comparative example provides a composition that differs from Example 1 only in that nisin is omitted. The reduced mass of nisin is proportionally distributed to the mass of azelaic acid and acetylcysteine, while the other components and their contents remain unchanged. The components are physically mixed uniformly according to their mass ratio.

[0059] Comparative Example 3

[0060] This comparative example provides a composition that differs from Example 1 only in that acetylcysteine ​​is omitted. The reduced mass of acetylcysteine ​​is proportionally distributed to the mass of azelaic acid and nisin, while the other components and their contents remain unchanged. The components are physically mixed uniformly according to their mass ratio.

[0061] Comparative Example 4

[0062] This comparative example provides a composition that differs from Example 1 only in that it does not contain recombinant collagen. The reduced mass of the collagen is allocated to the mass of azelaic acid, while the other components and their contents remain unchanged. The components are physically mixed uniformly according to the mass ratio.

[0063] Application Example 1

[0064] This application example provides a gel, the formula of which includes the following ingredients:

[0065] Its preparation method is:

[0066] (1) Mixing the gelling agent with a portion of water at 30°C to obtain solution A;

[0067] (2) Azelaic acid was mixed with 1,3-propylene glycol and butylene glycol at 40° C., and then mixed with nisin, acetylcysteine, recombinant collagen, a solubilizer, glycerol and the remaining water at 40° C. to obtain solution B;

[0068] (3) Solution A and solution B are mixed at 30° C., and triethanolamine is added to obtain the gel having acne-removing and repairing effects.

[0069] Application Example 2-3

[0070] This application example provides two gels. Their formulations differ from those of Application Example 1 only in that the acne-removing and repairing composition of Example 1 is replaced with an equal amount of the acne-removing and repairing composition prepared in Examples 2-3. All other components and amounts remain unchanged. Their preparation methods follow those of Application Example 1.

[0071] Comparative Application Examples 1-4

[0072] Comparative Application Examples 1-4 provide four gels. Their formulations differ from those of Application Example 1 only in that the acne-removing and repairing composition prepared in Example 1 is replaced with an equal amount of the acne-removing and repairing composition prepared in Comparative Examples 1-4. The other components and contents remain unchanged. Their preparation methods are similar to those of Application Example 1.

[0073] Application Example 4

[0074] This application example provides a gel, the formula of which includes the following ingredients:

[0075] Its preparation method is:

[0076] (1) Mixing the gelling agent with a portion of water at 35°C to obtain solution A;

[0077] (2) Azelaic acid was mixed with 1,3-propylene glycol and butylene glycol at 30°C, and then mixed with nisin, acetylcysteine, recombinant collagen, a solubilizer, glycerol and the remaining water at 30°C to obtain solution B;

[0078] (3) Solution A and solution B are mixed at 30° C., and triethanolamine and a conditioning agent are added to obtain the gel having acne-removing and repairing effects.

[0079] Application Example 5

[0080] This application example provides a gel, the formula of which differs from that of Application Example 4 only in that the Kapok flower extract is missing and the mass percentage of the Kapok flower extract is 8%, while the other components and contents remain unchanged.

[0081] Application Example 6

[0082] This application example provides a gel, the formula of which differs from that of Application Example 4 only in that the kapok flower extract is missing and the mass percentage of the Sufang flower extract is 8%, while the other components and contents remain unchanged.

[0083] Application Example 7

[0084] This application example provides a gel, the formula of which differs from that of Application Example 1 only in that it lacks the acne-repairing composition, the reduced mass of which is supplemented by water, and the other components and contents remain unchanged.

[0085] Test Example 1

[0086] Safety evaluation:

[0087] The products prepared in Application Examples 1-7 and Comparative Application Examples 1-4 were placed in a spot tester. The spot tester containing the test substance was attached to the flexor side of the forearm of 20 subjects. The tester was gently pressed with the palm of the hand to evenly apply the test substance to the skin for 24 hours. The skin irritation and sensitization were observed according to Table 1 30 minutes, 24 hours, and 48 hours after the removal of the test substance spot tester, and the observation results were recorded.

[0088] Table 1

[0089] The results show that the ratings of the products prepared in Application Examples 1-7 and Comparative Application Examples 1-4 are all 0, which means that the acne-removing and repairing composition involved in this application is safe and non-irritating to the skin.

[0090] Test Example 2

[0091] Propionibacterium acnes inhibition test:

[0092] Prepare sample solutions: Sample solutions 1-11 correspond to Application Examples 1-7 and Comparative Application Examples 1-4, respectively. Their formulations differ from those of Application Examples 1-7 and Comparative Application Examples 1-4 in that they do not contain a solubilizer, gelling agent, or glycerin; the reduced mass is supplemented by water. Prepare the solutions by mixing azelaic acid with 1,3-propylene glycol and butylene glycol at 30°C, then mixing with nisin, acetylcysteine, recombinant collagen, and water at 30°C. Optionally, a conditioning agent may be added and mixed thoroughly.

[0093] Propionibacterium acnes was 1×10 6 Cells were inoculated into a 96-well plate at 150 μL per well. 150 μL of each sample solution 1-11 was added. An equal volume of culture medium was added as a blank control. The cells were incubated in a 37°C incubator under anaerobic conditions for 48 hours. The absorbance at 600 nm was measured using a UV spectrophotometer, and the P. acnes inhibition rate (%) was calculated using the following formula. Five replicates were performed for each group, and the average value was calculated.

[0094] Propionibacterium acnes inhibition rate (%) = (blank group absorbance - test group absorbance) / blank group absorbance × 100%

[0095] The results are shown in Table 2.

[0096] Table 2

[0097] As can be seen from the data in Table 2, the acne-removing and repairing composition of the present application has a significant inhibitory effect on Propionibacterium acnes, and azelaic acid, nisin, and acetylcysteine ​​have a synergistic effect in inhibiting Propionibacterium acnes. The extract of Sufanghua and the extract of Kapok flower can further enhance the above effects, and the two have a synergistic promoting effect.

[0098] Test Example 3

[0099] Propionibacterium acnes inhibition test:

[0100] Prepare sample solutions: Sample solutions 1-11 correspond to Application Examples 1-7 and Comparative Application Examples 1-4, respectively. Their formulations differ from those of Application Examples 1-7 and Comparative Application Examples 1-4 in that they do not contain a solubilizer, gelling agent, or glycerin; the reduced mass is supplemented by water. Prepare the solutions by mixing azelaic acid with 1,3-propylene glycol and butylene glycol at 30°C, then mixing with nisin, acetylcysteine, recombinant collagen, and water at 30°C. Optionally, a conditioning agent may be added and mixed thoroughly.

[0101] 1×10 6 Raw264.7 cells (100 μL / mL) were seeded into 24-well plates, with 300 μL per well. The cells were incubated at 37°C for 24 hours, and the supernatant was discarded. The blank group was treated with 300 μL of fresh culture medium, the LPS group with 300 μL of fresh culture medium containing 1 μg / mL LPS, and the experimental groups with 300 μL of sample solutions 1-11 containing 1 μg / mL LPS. The cells were then incubated at 37°C for 24 hours. The supernatant was aspirated and centrifuged. The concentrations of the inflammatory factors TNF-α and IL-1 were measured according to the ELISA kit protocol. Five replicates were performed for each group, and the average values ​​were calculated. The results are shown in Table 3.

[0102] Table 3

[0103] It can be seen from the data in Table 3 that the acne-removing and repairing composition involved in the present application has a significant anti-inflammatory effect, and azelaic acid, nisin, and acetylcysteine ​​have a synergistic enhancing effect in inhibiting inflammatory factors. The extract of Sufanghua and the extract of Kapok flower can further enhance the above effects, and the two have a synergistic promoting effect.

[0104] Test Example 4

[0105] Sensory evaluation test:

[0106] A total of 160 female and male volunteers aged 18-40 with oily, acne-prone skin were randomly divided into eight groups, each consisting of 20 participants, half male and half female. Each group used the products from Application Examples 1, 4-6, and Comparative Application Examples 1-4, once daily, morning and evening, after cleansing, for 60 consecutive days. No other acne-removing products were used during this period, and the dosage and technique remained essentially the same. After 60 days, the products were rated for their mildness and acne-removing effects, with a maximum score of 5 and a minimum of 1, ranging from 1 to 5. The evaluation results are shown in Table 4 (average score):

[0107] Table 4

[0108] As can be seen from Table 4, the composition involved in the present application and the cosmetics prepared therefrom have both excellent acne-removing and repairing properties and mildness, among which recombinant collagen plays an important role in improving the acne-removing and repairing effect and mildness of the product, and the plant extracts added to the cosmetics can further enhance the acne-removing and repairing effect of the composition, and the Sufang flower extract and the kapok flower extract have a certain synergistic effect in improving the acne-removing and repairing effect of the product.

[0109] The applicant declares that while the above-mentioned embodiments are used to illustrate the technical solutions of this application, this application is not limited to these embodiments, and does not imply that this application must rely on these embodiments in order to be implemented. Persons skilled in the art should understand that any improvements to this application, equivalent replacements for the raw materials of the products of this application, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of this application.

[0110] The preferred embodiments of the present application are described in detail above. However, the present application is not limited to the specific details of the above embodiments. Within the technical concept of the present application, various simple modifications can be made to the technical solution of the present application, and these simple modifications all fall within the scope of protection of the present application.

[0111] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner unless there is any contradiction. In order to avoid unnecessary repetition, this application will not further describe various possible combinations.

Claims

1. An acne-removing and repairing composition containing recombinant collagen, comprising azelaic acid, nisin, acetylcysteine ​​and recombinant collagen.

2. The acne-removing and repairing composition containing recombinant collagen according to claim 1, wherein: The acne-removing and repairing composition comprises, by weight, 4-10 parts of azelaic acid, 0.1-1 parts of nisin, 0.5-5 parts of acetylcysteine ​​and 0.01-1 parts of recombinant collagen.

3. The acne-removing and repairing composition containing recombinant collagen according to claim 1, wherein: The nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO:

1.

4. Use of the acne-removing and repairing composition containing recombinant collagen according to any one of claims 1 to 3 in the preparation of acne-removing and repairing products.

5. The use according to claim 4, wherein: The amount of the acne-removing and repairing composition added to the acne-removing and repairing product is 1-20%; Optionally, the product comprises a cosmetic or a medicine.

6. A gel with acne-removing and repairing effects, wherein the raw materials for its preparation include, by weight percentage: 1-20% of the acne-repairing composition according to any one of claims 1 to 3, 0.5-5% of a gelling agent, 0.2-2% of a solubilizing agent, 5-20% of a polyol, 0.1-1% of triethanolamine and 50-90% of water.

7. The gel with acne-removing and repairing effects according to claim 6, wherein: The gel comprises carbomer; Optionally, the solubilizing agent comprises PPG-26-buteth-26 and / or PEG-40 hydrogenated castor oil; Optionally, the polyol includes any one of propylene glycol, butylene glycol or glycerol, or a combination of at least two of them.

8. The gel with acne-removing and repairing effects according to claim 6, wherein: The raw materials for preparing the gelling agent further include, by weight percentage, any one of 1-10% of plant-derived conditioning agent, 0.05-0.5% of antioxidant, or 0.1-0.5% of aromatic agent, or a combination of at least two of them.

9. The gel with acne-removing and repairing effects according to claim 8, wherein: The plant-derived conditioning agent includes a safflower flower extract and / or a kapok flower extract.

10. A method for preparing a gel having acne-removing and repairing effects according to any one of claims 6 to 9, comprising the following steps: The gel is mixed with part of water at 25-45° C. to obtain solution A; the acne-removing and repairing composition, the solubilizer, the polyol and the remaining water are mixed at 30-50° C. to obtain solution B; solution A and solution B are mixed at 20-40° C., triethanolamine and the remaining preparation raw materials are added to obtain the gel with acne-removing and repairing effect.

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