Method for producing artificial meat, and apparatus for performing same

Abstract

An apparatus for producing artificial meat by using bio-ink, according to one embodiment of the present application, comprises: a plurality of modules; and at least one processor for controlling the plurality of modules, wherein the plurality of modules include a monitoring module and an extrusion module, and the at least one processor can control that the state of the bio-ink maintains a predetermined condition by means of the monitoring module, and can control that at least a part of the bio-ink is extruded and extracted by means of the extrusion module.

Description

Method for producing artificial meat and device for performing the same

[0001] The present invention relates to a method for producing artificial meat or artificial organs and a device for performing the same, and relates to a method for producing artificial meat or artificial organs in large quantities in a short period of time by monitoring the state of bioink and extracting it by extrusion.

[0002]

[0003] The present invention relates to a device for manufacturing artificial meat or organs using bioink. Artificial meat is considered one of the most important future foods, an eco-friendly alternative that can contribute to curbing global warming. It can also address the massive greenhouse gas emissions generated by conventional meat production and serve as a sustainable food production method. Artificial organs are also highly important, as they can significantly contribute to extending human lifespan, improving health, and enhancing function.

[0004] Existing methods for manufacturing artificial meat and / or organs (hereinafter referred to as "artificial meat") have primarily relied on 3D printing technology to extract bioink. This process is slow and inefficient for large-scale production. Furthermore, artificial meat manufactured through this method has limitations in replicating the texture or cross-sectional shape of real meat. This could hinder consumers' acceptance of artificial meat as a comparable substitute for real meat.

[0005] There is a growing need to develop a technology that can overcome the limitations of conventional technologies, accurately monitor the status of bioink, extract large quantities of bioink, produce large quantities of artificial meat in a short period of time, and simultaneously reproduce the texture and appearance of real meat as closely as possible.

[0006]

[0007] One object of the present invention is to provide a method for mass-producing artificial meat that reproduces the texture and shape of actual meat in a short period of time by storing and monitoring bioink and extracting a large amount of bioink in a short period of time through an extrusion device.

[0008] The problems to be solved by the present invention are not limited to the problems described above, and problems not mentioned can be clearly understood by a person having ordinary skill in the technical field to which the present invention pertains from this specification and the attached drawings.

[0009]

[0010] A device for producing artificial meat using bioink disclosed in the present invention comprises a plurality of modules and at least one processor for controlling the plurality of modules, wherein the plurality of modules include a monitoring module and an extrusion module, and the at least one processor controls the state of the bioink to maintain a predetermined condition through the monitoring module, and controls at least a portion of the bioink to be extruded and extracted through the extrusion module.

[0011] The solutions to the problems of the present invention are not limited to the solutions described above, and solutions that are not mentioned can be clearly understood by a person having ordinary skill in the art to which the present invention pertains from this specification and the attached drawings.

[0012]

[0013] According to this embodiment, by extracting a large amount of bioink at once through an artificial meat production device, the speed of the artificial meat production process can be significantly improved, enabling large-scale production. This can make the commercial production of artificial meat more realistic and economically feasible.

[0014] According to this embodiment, by producing artificial meat by mass-extracting bio-ink in the form of fiber bundles, the appearance and texture of real meat can be reproduced as much as possible, and thus, not only the texture and appearance of artificial meat, but also the taste and nutritional value can be made more similar to real meat, thereby providing the effect of increasing consumer acceptance of artificial meat.

[0015] The effects of the invention are not limited to the effects described above, and effects not mentioned can be clearly understood by a person having ordinary skill in the art to which the present invention pertains from this specification and the attached drawings.

[0016]

[0017] FIG. 1 is a drawing for explaining an artificial meat manufacturing system according to one embodiment.

[0018] Figure 2 is a drawing for explaining the configuration of a manufacturing device according to one embodiment.

[0019] FIGS. 3 and 4 are drawings for explaining a plurality of modules constituting a manufacturing module according to one embodiment.

[0020] FIGS. 5 and 6 are drawings illustrating a pair of manufacturing modules according to one embodiment.

[0021] Figures 7 to 9 are drawings for explaining a monitoring module according to one embodiment.

[0022] FIG. 10 and FIG. 11 are drawings for explaining an extrusion module according to one embodiment.

[0023] Fig. 12 is a drawing showing a cross-section of a plunger of an extrusion module according to one embodiment.

[0024] Fig. 13 is a drawing for explaining the extrusion operation of a plunger according to one embodiment.

[0025] Fig. 14 is a drawing for explaining the filling operation of a plunger according to one embodiment.

[0026] FIG. 15 is a drawing for explaining a plunger of an extrusion module according to another embodiment.

[0027] Fig. 16 is a drawing for explaining the extrusion operation and filling operation of a plunger according to another embodiment.

[0028] FIG. 17 is a drawing for explaining a plunger of an extrusion module according to another embodiment.

[0029] FIG. 18 is a drawing for explaining the extrusion operation and filling operation of a plunger according to another embodiment.

[0030] Figures 19 and 20 are drawings for explaining a nozzle according to one embodiment.

[0031] FIG. 21 is a drawing for explaining an expandable manufacturing module according to one embodiment.

[0032] FIG. 22 is a drawing for explaining the arrangement and operation of an expandable manufacturing module according to one embodiment.

[0033] FIGS. 23 to 25 are drawings for explaining a monitoring module included in an expandable manufacturing module according to one embodiment.

[0034] FIG. 26 is a drawing for explaining a method for controlling an extrusion module based on user input according to one embodiment.

[0035] Fig. 27 is a drawing for explaining an extrusion module according to another embodiment.

[0036] Fig. 28 is a drawing for explaining a transport device according to one embodiment.

[0037] FIG. 29 is a drawing for explaining the flow of fluid within a transport device according to one embodiment.

[0038] FIGS. 30 and 31 are drawings for explaining the transport efficiency when using a transport device according to one embodiment.

[0039] Fig. 32 is a drawing for explaining a manufacturing device according to another embodiment.

[0040] Fig. 33 is a drawing for explaining a cell mixing device according to one embodiment.

[0041] Fig. 34 is a drawing for explaining the configuration of a cell mixing device according to one embodiment.

[0042] Fig. 35 is a drawing for explaining the detailed configuration of a stirring module according to one embodiment.

[0043] FIGS. 36 and 37 are drawings for explaining the operation of a cell mixing device according to one embodiment.

[0044] FIGS. 38 and 39 are drawings for explaining the operation method of a plurality of cell mixing modules and an extrusion material composition module according to one embodiment.

[0045] Figure 40 is a drawing for explaining a post-processing module according to one embodiment.

[0046] Fig. 41 is a drawing for explaining the configuration of a bridge module according to one embodiment.

[0047] FIG. 42 is a diagram illustrating a method for a processor to control the operation of a bridge module according to one embodiment.

[0048] Figure 43 is a drawing for explaining the configuration of a culture module according to one embodiment.

[0049] FIG. 44 is a diagram illustrating a method for a processor to control the operation of a culture module according to one embodiment.

[0050] FIGS. 45 and 46 are drawings for explaining a method for a processor to control a post-processing process of an extruded material according to one embodiment.

[0051] FIG. 47 is a diagram illustrating a method for a processor to control and monitor a plurality of modules constituting an automation system according to one embodiment.

[0052] FIGS. 48 and 49 are diagrams illustrating a method for a processor to manage the state of a culture module according to one embodiment.

[0053] FIG. 50 is a diagram illustrating a method for a processor to control a plurality of containers included in a culture module according to one embodiment.

[0054] FIGS. 51 and 52 are drawings illustrating an assembly module according to one embodiment.

[0055] Figure 53 is a drawing for explaining the configuration of an assembly module according to one embodiment.

[0056] FIGS. 54 to 56 are drawings for explaining a first method for manufacturing artificial meat by an assembly module according to one embodiment.

[0057] FIG. 57 is a drawing illustrating a second method for manufacturing artificial meat by an assembly module according to one embodiment.

[0058] FIG. 58 is a drawing illustrating how an assembly module according to one embodiment operates in multiple work areas.

[0059] FIGS. 59 to 61 are drawings for explaining a third method of manufacturing artificial meat by an assembly module according to one embodiment.

[0060] FIGS. 62 to 64 are drawings for explaining a method for manufacturing artificial meat having a predetermined pattern using an assembly module according to one embodiment.

[0061] FIGS. 65 and 66 are drawings for explaining a method for obtaining artificial meat by processing a unit structure including a plurality of layers by an assembly module according to one embodiment.

[0062] FIG. 67 is a drawing for explaining a cross-section of artificial meat manufactured by a manufacturing device according to one embodiment.

[0063]

[0064] The above-described purposes, features, and advantages of the present application will become more apparent through the following detailed description, taken in conjunction with the accompanying drawings. However, as the present application is susceptible to various modifications and various embodiments, specific embodiments will be illustrated in the drawings and described in detail below.

[0065] Throughout the specification, identical reference numbers, in principle, indicate identical components. Furthermore, components with identical functions within the scope of the same concept shown in the drawings of each embodiment are described using the same reference numbers, and redundant descriptions thereof will be omitted.

[0066] If a detailed description of a known function or configuration related to this application is deemed to unnecessarily obscure the gist of this application, such detailed description will be omitted. Furthermore, numbers (e.g., "first," "second," etc.) used throughout the description of this specification are merely identifiers used to distinguish one component from another.

[0067] In addition, the suffixes "module" and "part" for components used in the following examples are given or used interchangeably only for the convenience of writing the specification, and do not have distinct meanings or roles in themselves.

[0068] In the examples below, singular expressions include plural expressions unless the context clearly indicates otherwise.

[0069] In the examples below, terms such as “include” or “have” mean that a feature or component described in the specification is present, and do not preclude the possibility that one or more other features or components may be added.

[0070] For convenience of explanation, the sizes of components in the drawings may be exaggerated or reduced. For example, the sizes and thicknesses of each component shown in the drawings are arbitrarily shown for convenience of explanation, and the present invention is not necessarily limited to what is shown.

[0071] In some embodiments, where implementations are otherwise feasible, the order of specific processes may differ from the order described. For example, two processes described in succession may be performed substantially simultaneously, or in a reverse order from the order described.

[0072] In the following examples, when components are said to be connected, this includes not only cases where the components are directly connected, but also cases where components are interposed between the components and are indirectly connected.

[0073] For example, when it is said in this specification that components, etc. are electrically connected, it includes not only cases where the components, etc. are directly electrically connected, but also cases where components, etc. are interposed in between and are indirectly electrically connected.

[0074]

[0075] This specification describes a method for producing artificial meat and / or artificial organs. However, since the methods for producing artificial meat and the methods for producing artificial organs disclosed in this specification largely correspond to each other, for convenience of explanation, "artificial meat and / or artificial organs" will be expressed as "artificial meat." Unless otherwise specified, "artificial meat" should be interpreted as a term that includes "artificial meat and / or artificial organs," and if not, it will be described with a separate explanation.

[0076]

[0077] FIG. 1 is a drawing for explaining an artificial meat manufacturing system according to one embodiment.

[0078] Referring to FIG. 1, an artificial meat manufacturing system according to one embodiment may include a manufacturing device (100), a control server (200), an extrusion material production device (300), and a user terminal (400).

[0079] According to one embodiment, the extrusion material production device (300) relates to a device for producing bio-ink, which serves as the basis for producing artificial meat, and the manufacturing device (100) relates to a device for producing artificial meat using bio-ink. The user terminal (400) may be an electronic device that receives user input for producing customized artificial meat. The control server (200) may be an electronic device that controls at least one of the components constituting the system.

[0080] The extrusion material production device (300) can produce one or more types of bioinks according to predetermined criteria. The extrusion material production device (300) can include a device for producing fat-related bioinks and a device for producing protein-related bioinks.

[0081] The manufacturing device (100) can perform the functions of storing bio-ink produced through the extrusion material production device (300), monitoring the status of bio-ink, and extracting bio-ink. The manufacturing device (100) can include a plurality of modules that perform the functions described above, and a more detailed description of the plurality of modules will be described later with reference to the drawings.

[0082] The control server (200) can perform an operation to control the function of the extrusion material production device (300) and / or the manufacturing device (100). The control server (200) is communicatively connected to the extrusion material production device (300) and / or the manufacturing device (100) and can perform an operation to control the respective functions. The control server (200) can control the operation of the extrusion material production device (300) and / or the manufacturing device (100) based on a control signal received from a user terminal (400).

[0083] The user terminal (400) may be an electronic device that receives user input and may receive information regarding artificial meat of a desired form. The user may input desired conditions (e.g., the shape, appearance, texture, and ratio (ratio of fat and protein) of the artificial meat, etc.) through the user terminal (400), and the control server (200) may control the operation of the manufacturing device (100) so that the artificial meat can be produced based on the information regarding the conditions.

[0084] FIG. 2 is a drawing for explaining the configuration of a manufacturing device according to one embodiment. Referring to FIG. 2, the manufacturing device (100) according to one embodiment may include at least one module.

[0085] A manufacturing device (100) according to one embodiment may include at least one of a manufacturing module (1000), a processor (2000), a communication module (3000), a memory (4000), an output module (5000), and a user interface (6000).

[0086] The manufacturing device (100) may include a manufacturing module (1000). The manufacturing module (1000) may perform operations of storing, monitoring, extruding, and / or crosslinking bioink, and may be composed of multiple modules. A more detailed description of the multiple modules constituting the manufacturing module (1000) will be described later with reference to the drawings.

[0087] The manufacturing device (100) may include a processor (2000). The manufacturing device (100) may include at least one processor, and may control the operations of elements constituting the manufacturing device (100) through the processor (2000). The processor (2000) may communicate with other elements within the device by executing software instructions, and may interpret and execute input user commands.

[0088] The manufacturing device (100) may include a communication module (3000). The communication module (3000) may be communicatively connected to an external device or network and may perform a function of transmitting and receiving data. The communication module (3000) may enable information exchange with a cloud server or other devices through a communication connection, and may assist in functions such as real-time monitoring of bioink status, software updates, optimization of the manufacturing process, and remote control.

[0089] The manufacturing device (100) may include a memory (4000). The manufacturing device (100) may store commands to be executed by the processor (2000) and information necessary for monitoring the status of bioink through the memory (4000).

[0090] The manufacturing device (100) may include an output module (5000). The output module (5000) may transmit information about events occurring during the artificial meat production process (e.g., progress of the manufacturing process, warning messages, quality assessment of the finished product, etc.) to a user through the output module (5000). The output module (5000) may be implemented in various forms, such as a visual output device or an auditory output device.

[0091] The manufacturing device (100) may include a user interface (6000). The manufacturing device (100) may receive user input through the user interface (6000) and control the operation of the device based on the input user input. The user interface (6000) may take the form of various input tools such as a touch screen, a button, a keyboard, a mouse, etc., and the user may control the start and stop of the manufacturing operation through the user interface (6000) and may also set parameters such as the type of bioink, extrusion speed, and temperature.

[0092]

[0093] FIGS. 3 and 4 are drawings for explaining a plurality of modules constituting a manufacturing module according to one embodiment.

[0094] Referring to FIG. 3, a manufacturing module (1000) according to one embodiment may include a plurality of modules. The manufacturing module (1000) may include a first module (1100), a second module (1200), a third module (1300), and a fourth module (1400). Each module may perform an operation of storing bioink, an operation of monitoring the status of bioink, an operation of extruding bioink, and an operation of crosslinking bioink.

[0095] Referring to FIG. 4, the first module may be a storage module (1100), the second module may be a monitoring module (1200), the third module may be an extrusion module (1300), and the fourth module may be a post-processing module (1400).

[0096] The storage module (1100) may be a module for storing bioink. The storage module (1100) may be in the form of a tank having an internal space. The storage module (1100) may be in the form of a large tank (bulk tank) capable of storing a large amount of bioink.

[0097] The monitoring module (1200) may be a module that monitors the status of bioink. The monitoring module (1200) may monitor the status of bioink based on at least one indicator. The monitoring module (1200) may monitor the temperature or pH value of the bioink. The monitoring module (1200) will be described later with reference to the drawings.

[0098] The extrusion module (1300) can perform the function of extruding bioink. The extrusion module (1300) can extrude bioink by applying pressure to the bioink via a syringe. The extrusion module (1300) can extrude a large amount of bioink in the form of a fiber bundle, and a more detailed description thereof will be described later with reference to the drawings.

[0099] The post-processing module (1400) may have an internal space. The post-processing module (1400) may have an internal space capable of accommodating a cross-linking solution of a predetermined capacity. The post-processing module (1400) may be in the form of a tank or a water tank having an internal space, and the cross-linking solution may be stored in the internal space. The cross-linking solution may be an aqueous solution containing a chemical. The cross-linking solution may be calcium chloride (CaCl2), but is not limited thereto. The cross-linking solution may also be a solution having a predetermined temperature (e.g., tap water, purified water, etc.).

[0100] The post-processing module (1400) may include at least one sensor, and may monitor the state of the cross-linking solution stored in the internal space using the at least one sensor. For example, the post-processing module (1400) may be equipped with an electrical conductivity sensor, and may monitor the state of the cross-linking solution stored in the internal space based on the electrical conductivity sensor. The post-processing module (1400) may measure the contamination concentration of the internal space based on the electrical conductivity sensor, and the processor (2000) may control the cross-linking solution to be circulated and replaced if it is determined that the contamination concentration exceeds a certain level.

[0101] The post-processing module (1400) may be provided in a form that is detachable from the manufacturing module (1000). Since the cross-linking solution stored in the post-processing module (1400) needs to be replaced after a certain period of time or when a predetermined standard is met, the post-processing module (1400) may be provided in a form that is detachable and mountable in a cart form that is detachable from the manufacturing module (1000). The post-processing module (1400) may be in the form of a transparent case tank.

[0102] The post-processing module (1400) may be equipped with a circulation system. The post-processing module (1400) may replace the cross-linking solution stored in the internal space through the circulation system after a certain period of time or when a predetermined standard is met. The post-processing module (1400) may be equipped with a pump and pipelines, which may be used to operate the circulation system.

[0103]

[0104] FIGS. 5 and 6 are drawings illustrating a pair of manufacturing modules according to one embodiment. Referring to FIGS. 5 and 6, the manufacturing module (1000) may include a plurality of storage modules, a plurality of monitoring modules, a plurality of extrusion modules, and a plurality of post-processing modules.

[0105] In one embodiment, the bioinks may be of multiple types. For example, the bioinks may include a first bioink containing fat and a second bioink containing protein.

[0106] The storage module (1100) may include a first storage module for storing the first bio-ink and a second storage module for storing the second bio-ink. The monitoring module (1200) may include a first monitoring module (1200a) for monitoring the first bio-ink and a second monitoring module (1200b) for monitoring the second bio-ink. The extrusion module (1300) may include a first extrusion module (1300a) for extruding the first bio-ink and a second extrusion module (1300b) for extruding the second bio-ink. The post-processing module (1400) may include a first post-processing module (1400a) for cross-linking the first bio-ink and a second post-processing module (1400b) for cross-linking the second bio-ink.

[0107] When the first bio-ink and the second bio-ink are produced from the extrusion material production device (300), the first bio-ink can be stored in the first storage module. The first monitoring module (1200a) monitors the status of the first bio-ink being transported through the pump, and the first extrusion module (1300a) can extrude the first bio-ink in the form of a fiber bundle. The first post-processing module (1400a) can perform a function of cross-linking the extruded first bio-ink.

[0108] Likewise, the second bioink produced from the extrusion material production device (300) can be stored in the second storage module. The second monitoring module (1200b) monitors the status of the second bioink being transported via the pump, and the second extrusion module (1300b) can extrude the second bioink in the form of a fiber bundle. The second post-processing module (1400b) can perform a function of crosslinking the extruded second bioink.

[0109] The above-described pair of storage modules (1100), monitoring modules (1200), extrusion modules (1300), and post-processing modules (1400) can be arranged symmetrically. The pair of storage modules (1100), monitoring modules (1200), extrusion modules (1300), and post-processing modules (1400) can be controlled in parallel.

[0110]

[0111] FIGS. 7 to 9 are drawings illustrating a monitoring module according to one embodiment. Referring to FIGS. 7 to 9, a monitoring module (1200) according to one embodiment may include an outer housing (1210) and an inner housing (1220).

[0112] The monitoring module (1200) may have an internal space capable of accommodating bio-ink, and the internal space may be formed based on at least one of an external housing (1210) and an internal housing (1220). The external housing (1210) and the internal housing (1220) may form an internal space, and the internal housing (1220) may be located inside the external housing (1210). The monitoring module (1200) may accommodate bio-ink in the internal space formed by the internal housing (1220).

[0113] The monitoring module (1200) may be equipped with a blade (1230). The blade (1230) may be positioned in the internal space of the internal housing (1220). The blade (1230) may be in the form of an impeller and may perform a function of stirring bio-ink stored inside the internal housing (1220) by rotating by power.

[0114] The blade (1230) may be positioned so as to be spaced apart from the inner surface of the inner housing (1220) by a predetermined distance. For example, when the blade (1230) rotates by power, a first radius formed on a plane may be smaller than a second radius of the inner housing (1220), but may be formed within a predetermined numerical range.

[0115] When the first radius is formed within a predetermined numerical range relative to the second radius, more efficient stirring can occur. For example, the difference between the first and second radii may be 10 mm, in which case the blade (1230) rotates in closer contact with the inner surface of the inner housing (1220), thereby more effectively performing the stirring action of the bioink.

[0116] The shape of the blade (1230) may be provided in various shapes (e.g., a U shape) other than the shape illustrated in the drawing. When the blade (1230) is provided in a U shape, the area of ​​the blade (1230) that comes into contact with the inner surface of the inner housing (1220) increases, thereby enabling a more effective bio-ink stirring operation.

[0117]

[0118] The monitoring module (1200) may include at least one sensor (1240). The monitoring module (1200) may include at least one sensor (1240) capable of monitoring the status of bioink stored in the internal housing (1220). The monitoring module (1200) may include a temperature sensor capable of measuring the temperature value of the bioink and / or a pH sensor (or pH measuring device) capable of measuring the pH value.

[0119] The monitoring module (1200) may include a reagent pump (1250). The monitoring module (1200) may control the state of the bioink stored in the internal housing (1220) through the reagent pump (1250). For example, the monitoring module (1200) may monitor the pH value of the bioink stored in the internal housing (1220) based on at least one sensor (1240), and if the measured pH value is determined to be outside a predetermined condition, the monitoring module (1200) may adjust the pH of the bioink through the reagent pump (1250).

[0120] The pH of bioink can be a critical factor in determining the quality of artificial meat. It is important to monitor and control the pH of bioink to ensure it remains at an appropriate level (e.g., neutral). Therefore, the manufacturing device (100) can monitor and control the pH of stored bioink to maintain it at an appropriate level via a monitoring module (1200).

[0121]

[0122] The monitoring module (1200) may have a double-jacket structure formed by an outer housing (1210) and an inner housing (1220). The monitoring module (1200) may include a multi-layer space corresponding to the double-jacket structure. The multi-layer space may be a space formed between the outer housing (1210) and the inner housing (1220).

[0123] The monitoring module (1200) may include a circulator (1260). The circulator (1260) may be a device for maintaining the state of the bioink according to predetermined conditions. The circulator (1260) may be equipped with a fluid circulation mechanism, which may be controlled to maintain the temperature of the bioink at a uniform level.

[0124] The monitoring module (1200) can control the state of the bio-ink stored in the housing through the circulator (1260). For example, the monitoring module (1200) can control the temperature value of the bio-ink stored in the internal housing (1220) by circulating fluid through the multi-layer space using the circulator (1260).

[0125] More specifically, the monitoring module (1200) monitors the temperature value of the bio-ink measured by at least one sensor (1240), and if the measured temperature value is determined to be outside a predetermined condition, the temperature of the bio-ink can be controlled through the circulator (1260).

[0126] The monitoring module (1200) may include a heating block. The monitoring module (1200) may control the state of bioink stored in the housing through the heating block. For example, the monitoring module (1200) may monitor the temperature value of the bioink measured by at least one sensor (1240) and control the heating block so that the measured temperature value remains constant. The heating block may refer to a device that compensates for the temperature by transferring heat generated from the heating element to the block.

[0127] The monitoring module (1200) may include a Peltier system including a Peltier element. The monitoring module (1200) may include an electrical or water-cooled Peltier system. The monitoring module (1200) may control the state of bio-ink stored in the housing using the Peltier system. For example, the monitoring module (1200) may monitor the temperature value of the bio-ink measured by at least one sensor (1240) and control the Peltier system so that the measured temperature value is maintained constant.

[0128] The monitoring module (1200) can control the state of the bio-ink through a combination of at least one of a circulating system, a heating block, and a Peltier system based on the above-described double jacket structure.

[0129] The monitoring module (1200) may include a UV lamp (1270). The monitoring module (1200) may perform a sterilization operation using the UV lamp (1270). The UV lamp (1270) may be placed in the external housing (1210) or the internal housing (1220) and may perform a sterilization operation on bio-ink stored in the internal space.

[0130]

[0131] Figures 10 and 11 are drawings for explaining an extrusion module according to one embodiment. The extrusion module (1300) according to one embodiment may be a device that applies pressure to bioink to extract it in the form of a plurality of fiber bundles.

[0132] The extrusion module (1300) may include a syringe (1310) forming an internal space, a plunger (1320) disposed in the internal space of the syringe, and a nozzle (1330) disposed at the end of the plunger (1320).

[0133] An internal space is formed in the syringe (1310) of the extrusion module (1300), and bioink can be accommodated in the internal space. At least a portion of the bioink stored in the internal housing (1220) of the monitoring module (1200) can be moved by an external force into the internal space of the syringe (1310).

[0134] At least a portion of the bioink contained in the syringe (1310) of the extrusion module (1300) may be extruded and extracted in the form of a fiber bundle. The plunger (1320) may perform a power-driven upward or downward motion, and at least a portion of the bioink may be extruded and extracted by the upward or downward motion of the plunger (1320).

[0135] A nozzle (1330) may be arranged at the end of the plunger (1320), and the nozzle (1330) may have a plurality of holes or slits formed therein with a predetermined diameter. The extrusion module (1300) controls the rising or falling motion of the plunger (1320) to extrude at least a portion of the bio-ink so that it passes through the nozzle (1330) having the plurality of holes formed therein, thereby extracting the bio-ink in the form of a fiber bundle.

[0136]

[0137] Fig. 12 is a drawing showing a cross-section of a plunger of an extrusion module according to one embodiment. Referring to Fig. 12, the plunger (1320) may have a plurality of internal spaces. The plunger (1320) may include a first internal space (s1), a second internal space (s2), and a third internal space (s3). Here, the diameter (or width) of the first internal space (s1) may be formed to be smaller than the diameter (or width) of the second internal space (s2). The first internal space (s1) may be defined as a first layer, the second internal space (s2) may be defined as a second layer, and the third internal space (s3) may be defined as a third layer. Hereinafter, for convenience of explanation, the first layer to the third layer are described interchangeably as the first internal space to the third internal space.

[0138] A plate (pl) having at least one hole may be arranged in the second internal space (s2). The plate (pl) may move up and down within the second internal space (s2) in response to the rising or falling motion of the plunger (1320). The shape of the at least one hole provided in the plate (pl) may vary.

[0139] A plate (pl) may be provided with a first hole (h1) and a second hole (h2). The first hole (h1) and the second hole (h2) may be formed at a position spaced apart from the center of the plate (pl) by a first length (d1). At this time, the radius of the first internal space (s1) may be a second length (d2), and the first length (d1) may be formed longer than the second length (d2). The first hole (h1) and the second hole (h2) may be formed outside a first radius range formed based on the center of the plate (pl), and the first radius range may be greater than or equal to the radius of the first internal space (s1).

[0140] By the movement (rising or falling) of the plate (pl), the movement passage inside the plunger (1320) can be opened or blocked. Hereinafter, with reference to the drawings, the function of blocking or opening the movement path of the fluid according to the movement of the plunger (1310) and the plate (pl) will be described.

[0141]

[0142] Fig. 13 is a drawing for explaining an extrusion operation of a plunger according to one embodiment, and Fig. 14 is a drawing for explaining a filling operation of a plunger according to one embodiment. Referring to Figs. 13 and 14, movement occurs in a plate located in a second internal space corresponding to the movement direction of the plunger (1320), and thus the movement direction of the bioink can be determined.

[0143] Referring to FIG. 13, when the extrusion motion of the plunger (1320) is performed, the plate may move in a first direction, thereby causing at least some of the bioink stored in the syringe to pass through the hole of the plate and move in the first direction. The extrusion motion may be a motion in which the plunger (1320) moves in the first direction inside the syringe (1310).

[0144] When the extrusion operation of the plunger (1320) is performed, the plate moves in the first direction, so that the first internal space (s1), the second internal space (s2), and the third internal space (s3) of the plunger (1320) can be fluidly connected.

[0145] When the extrusion motion of the plunger (1320) is performed, as shown in (b) of FIG. 13, the plate moves in the first direction by pressure, so that at least a portion of the bio-ink stored inside the syringe (1310) can pass through the hole formed in the plate and move in the first direction. The extrusion motion may be a motion in which the plunger (1320) moves in the first direction inside the syringe (1310).

[0146] When the extrusion motion of the plunger (1320) is performed, as shown in (b) of FIG. 13, the plate moves in the first direction by pressure, thereby opening a first movement path through which at least a portion of the bioink stored inside the syringe (1310) can move to the post-processing module (1400). Here, the first direction may be the direction of the arrows in (a) and (b) of FIG. 13.

[0147] Referring to Fig. 14, when the filling operation of the plunger (1320) is performed, the plate may move in a second direction, thereby blocking the movement of the bioink in the second direction. The filling operation may be an operation in which the plunger (1320) moves in the second direction inside the syringe (1310). Here, the second direction may be the direction of the arrow in (b) of Fig. 14.

[0148] When the filling operation of the plunger (1320) is performed, the plate moves in the second direction, so that the first internal space (s1) and the second internal space (s2) of the plunger (1320) can be fluidly connected by the plate.

[0149] When the filling operation of the plunger (1320) is performed, as shown in (b) of FIG. 14, the plate moves in the second direction by pressure, so that a closed area formed on the plate can shield the first internal space (s1) of the plunger (1320). This shielding operation of the plunger (1320) can prevent the bioink from flowing back into the syringe (1310). Here, the closed area may refer to an area on the plane of the plate that is not a hole.

[0150] When the filling operation of the plunger (1320) is performed, the first movement passage described above can be blocked by the plate moving in the second direction by pressure as shown in (b) of Fig. 14.

[0151] When the filling operation of the plunger (1320) is performed, the shielding operation of the plunger (1320) described above can be performed, and the first moving passage described above can be blocked, thereby allowing the internal space of the syringe (1310) to be maintained in a vacuum state. At the same time, by blocking the inflow of the cross-linking solution stored in the post-processing module (1400) into the syringe (1310), cross-linking of the bio-ink stored inside the syringe (1310) can be prevented.

[0152] In other words, by repeatedly performing the extrusion and filling operations of the plunger (1320), the operation of extruding at least a portion of the bio-ink stored inside the syringe (1310) and extracting it to the outside (e.g., the post-processing module (1400)) and the operation of filling the bio-ink back into the syringe (1310) can be repeatedly performed.

[0153]

[0154] FIG. 15 is a drawing for explaining a plunger of an extrusion module according to another embodiment, and FIG. 16 is a drawing for explaining an extrusion operation and a filling operation of a plunger according to another embodiment.

[0155] Referring to (a) of FIG. 15, the plunger (1320) of the extrusion module (1300) may be provided as a ball spring valve or check valve type. The plunger (1320) may include at least one hole that functions as a passage through which a fluid moves. The plunger (1320) may include at least one hole through which bioink can move. For example, the plunger (1320) may include a first hole (h1) and a second hole (h2) through which bioink can move.

[0156] Referring to (b) of FIG. 15, the plunger (1320) of the extrusion module (1300) may include at least one ball and at least one spring. The ball and spring may be arranged inside a housing constituting the plunger (1320), and may be arranged on a path along which a fluid passes through a hole.

[0157] The above balls and springs may be provided as a pair. For example, the plunger (1320) may include a first ball (b1) on a fluid movement path formed by a first hole (h1), a second ball (b2) on a fluid movement path formed by a second hole (h2), a first spring at a position corresponding to the first ball (b1), and a second spring at a position corresponding to the second ball (b2).

[0158] Referring to FIG. 16, in response to the rising or falling motion of the plunger (1320), movement occurs in at least one ball (b1, b2) inside the plunger (1320), and as a result, a passage, which is a space through which a fluid moves, may be opened or blocked.

[0159] When a movement (rising movement) in which the plunger (1320) moves in the first direction (d1) as in (a) of Fig. 16 is performed, at least one ball (b1, b2) inside the plunger (1320) may move in the second direction (d2) by pressure, and at this time, the first direction and the second direction may be opposite directions.

[0160] By moving at least one ball (b1, b2) in the second direction (d2), the first internal space (s1) and the second internal space (s2), which are internal spaces of the syringe (1310) separated by the plunger (1320), can be fluidly connected to each other. By fluidly connecting the first internal space (s1) and the second internal space (s2), the movement passage inside the plunger (1320) can be opened. By fluidly connecting the first internal space (s1) and the second internal space (s2), at least a portion of the bio-ink stored inside the syringe (1310) can move in the second direction through the movement passage.

[0161] In this way, when the plunger (1320) moves in the first direction, at least some of the bio-ink stored inside the syringe (1310) moves in the second direction, thereby allowing an operation of filling the bio-ink to be performed.

[0162] When a movement (lowering movement) in which the plunger (1320) moves in the second direction as in (b) of Fig. 16 is performed, at least one ball (b1, b2) inside the plunger (1320) may move in the first direction (d1) by pressure, and at this time, the first direction and the second direction may be opposite directions.

[0163] As the at least one ball (b1, b2) moves in the first direction (d1), the first internal space (s1) and the second internal space (s2), which are internal spaces of the syringe (1310) separated by the plunger (1320), can be fluidically blocked from being connected. As the first internal space (s1) and the second internal space (s2) are fluidically blocked, the movement passage within the plunger (1320) can be blocked. As the first internal space (s1) and the second internal space (s2) are fluidically blocked, the bio-ink stored within the syringe (1310) cannot move in the second direction through the movement passage.

[0164] In this way, when the plunger (1320) moves in the second direction, the bio-ink stored inside the syringe (1310) cannot move in the second direction, and thus, an extrusion operation of the bio-ink through the nozzle (1330) can be performed. More specifically, when the plunger (1320) moves in the second direction, an extrusion operation can be performed on at least a portion of the bio-ink stored in the second internal space (s2), and at the same time, the bio-ink stored in the first internal space (s1) can be blocked from flowing into the second internal space (s2).

[0165]

[0166] FIG. 17 is a drawing for explaining a plunger of an extrusion module according to another embodiment, and FIG. 18 is a drawing for explaining an extrusion operation and a filling operation of a plunger according to another embodiment.

[0167] Referring to FIG. 17, the plunger (1320) may include a first plunger body (pb1) and a second plunger body (pb2). At least one hole may be formed in each of the first plunger body (pb1) and the second plunger body (pb2). The first plunger body (pb1) and the second plunger body (pb2) may be formed to be detachable or connectable to each other.

[0168] The plunger (1320) may include a disk (di), and the disk (di) may be arranged in an internal space formed by the first plunger body (pb1) and the second plunger body (pb2). At least one hole may be formed in the disk (di). At least one hole of the disk (di) may be formed at a position corresponding to a hole of the second plunger body (pb2).

[0169] The plunger (1320) may further include a spring (sp), and the disk (di) may be arranged to move in the internal space in response to the movement of the plunger (1320) by the spring (sp).

[0170] Referring to FIG. 18, in response to the rising or falling motion of the plunger (1320), movement occurs in the disk (di) included in the plunger (1320), and as a result, a passage, which is a space through which a fluid moves, may be opened or blocked.

[0171] When the plunger (1320) moves in the first direction (d1) as shown in (a) of Fig. 18 (rising motion), the disk (di) may move in the second direction (d2) by pressure, and at this time, the first direction and the second direction may be opposite directions.

[0172] As the disk (di) moves in the second direction (d2), the first internal space (s1) and the second internal space (s2), which are internal spaces of the syringe (1310) separated by the plunger (1320), can be fluidly connected to each other. As the first internal space (s1) and the second internal space (s2) are fluidly connected, the movement passage inside the plunger (1320) can be opened. As the first internal space (s1) and the second internal space (s2) are fluidly connected, at least a portion of the bio-ink stored inside the syringe (1310) can move in the second direction through the movement passage.

[0173] In this way, when the plunger (1320) moves in the first direction, at least some of the bio-ink stored inside the syringe (1310) moves in the second direction, thereby allowing an operation of filling the bio-ink to be performed.

[0174] When the plunger (1320) moves in the second direction (d2) as in (b) of Fig. 18 (lowering motion), the disk (di) may be moved in the first direction (d1) by pressure, and at this time, the first direction and the second direction may be opposite directions.

[0175] As the above disk (di) moves in the first direction (d1), the first internal space (s1) and the second internal space (s2), which are internal spaces of the syringe (1310) separated by the plunger (1320), can be fluidically blocked from being connected. As the first internal space (s1) and the second internal space (s2) are fluidically blocked, the movement passage inside the plunger (1320) can be blocked. As the first internal space (s1) and the second internal space (s2) are fluidically blocked, the bio-ink stored inside the syringe (1310) cannot move in the second direction through the movement passage.

[0176] In this way, when the plunger (1320) moves in the second direction, the bio-ink stored inside the syringe (1310) cannot move in the second direction, and thus, an extrusion operation of the bio-ink through the nozzle (1330) can be performed. More specifically, when the plunger (1320) moves in the second direction, an extrusion operation can be performed on at least a portion of the bio-ink stored in the second internal space (s2), and at the same time, the bio-ink stored in the first internal space (s1) can be blocked from flowing into the second internal space (s2).

[0177]

[0178] Figures 19 and 20 are drawings for explaining a nozzle according to one embodiment.

[0179] Referring to (a) of FIG. 19, a nozzle (1330) according to one embodiment may include a plurality of holes. The plurality of holes included in the nozzle (1330) may be formed to have a predetermined pattern. The plurality of holes included in the nozzle (1330) may be formed to be spaced apart from each other by a predetermined interval. The plurality of holes included in the nozzle (1330) may be formed to be spaced apart from each other by the same distance.

[0180] Referring to (b) of FIG. 19, a nozzle (1330) according to one embodiment may include a plurality of holes formed in a longitudinal direction. The plurality of holes formed in the nozzle (1330) may be formed to have a predetermined height. The plurality of holes formed in the nozzle (1330) may function as a passage through which a fluid moves.

[0181] The plurality of holes formed in the nozzle (1330) may have a Y-shaped cross-section. One side of the hole formed in the nozzle (1330) may be formed to have a first radius (d1), and the other side may be formed to have a second radius (d2). The hole formed in the nozzle (1330) may be formed such that an inlet portion may have a first radius (d1), and an outlet portion may have a second radius (d2). In this case, the first radius (d1) may be larger than the second radius (d2). For example, one side of the hole may be formed to have a diameter of 0.45 cm, and the other side of the hole may be formed to have a diameter of 0.3 cm.

[0182] Referring to FIG. 20, the plurality of holes included in the nozzle (1330) may be circular, but is not limited thereto, and may have various shapes such as polygons, straight lines, slits, etc. The shape of the extracted bio-ink may vary depending on the shape of the plurality of holes formed in the nozzle (1330).

[0183]

[0184] FIG. 21 is a drawing illustrating an expandable manufacturing module according to one embodiment. Referring to FIG. 21, the expandable manufacturing module (1000b) may include a plurality of modules.

[0185] The manufacturing module (1000b) may include a 1-1 module (1100a), a 1-2 module (1100b), a 2-1 module (1200a), a 2-2 module (1200b), a 3-1 module (1300a), a 3-2 module (1300b), a 4-1 module (1400a), and a 4-2 module (1400b).

[0186] The 1-1 module (1100a) and the 1-2 module (1100b) may correspond to the storage module (1100) described with reference to FIG. 4. For example, the 1-1 module (1100a) may be a first storage module, and the 1-2 module (1100b) may be a second storage module. Since the morphology or functional configuration of the first storage module and the second storage module may correspond to the storage module (1100) described above with reference to FIG. 4, a duplicate description will be omitted.

[0187] The 2-1 module (1200a) and the 2-2 module (1200b) may correspond to the monitoring module (1200) described with reference to FIG. 4. For example, the 2-1 module (1200a) may be the second monitoring module, and the 2-2 module (1200b) may be the second monitoring module. Since the morphological or functional configuration of the 1st monitoring module and the 2nd monitoring module may correspond to the monitoring module (1200) described above with reference to FIG. 4, a duplicate description will be omitted.

[0188] The 3-1 module (1300a) and the 3-2 module (1300b) may correspond to the extrusion module (1300) described with reference to FIG. 4. For example, the 3-1 module (1300a) may be a first extrusion module, and the 3-2 module (1300b) may be a second extrusion module. Since the morphological or functional configurations of the first extrusion module and the second extrusion module may correspond to those of the extrusion module (1300) described above with reference to FIG. 4, any overlapping descriptions will be omitted.

[0189] The 4-1 module (1400a) and the 4-2 module (1400b) may correspond to the post-processing module (1400) described with reference to FIG. 4. For example, the 4-1 module (1400a) may be a first post-processing module, and the 4-2 module (1400b) may be a second post-processing module. Since the first post-processing module and the second post-processing module may correspond in form or function to the post-processing module (1400) described above with reference to FIG. 4, a redundant description thereof will be omitted.

[0190] The manufacturing module (1000b) may include a first unit composed of a 1-1 module (1100a), a 2-1 module (1200a), a 3-1 module (1300a), and a 4-1 module (1400a), and a second unit composed of a 1-2 module (1100b), a 2-2 module (1200b), a 3-2 module (1300b), and a 4-2 module (1400b). The first unit and the second unit may perform operations related to different types of bio-inks. For example, the first unit may perform operations related to a first type of bio-ink, and the second unit may perform operations related to a second type of bio-ink. The first type of bio-ink may be related to fat, and the second type of bio-ink may be related to protein.

[0191] By including a plurality of modules formed by a pair of combinations, the manufacturing module (1000b) can enable simultaneous mass production of a larger quantity of artificial meat and / or artificial organs.

[0192]

[0193] FIG. 22 is a drawing illustrating the arrangement and operation of an expandable manufacturing module according to one embodiment. Referring to FIG. 22, the expandable manufacturing module (1000b) may include a pair of PLC (Programmable Logic Controller) boxes. The manufacturing module (1000b) may include a first PLC box (1010a) and a second PLC box (1010b).

[0194] The above PLC box can be used to automate and control the operations of various devices and modules in the manufacturing process. The manufacturing module (1000b) can control the operations of at least one of a circulator, a monitoring module, an extrusion module, and a post-processing module through a pair of PLC boxes. A user can automatically perform the operations of at least one of the circulator, the monitoring module, the extrusion module, and the post-processing module through a pair of PLC boxes by programming in advance.

[0195] The manufacturing module (1000b) may include a pair of circulators. The manufacturing module (1000b) may include a first circulator (1020a) and a second circulator (1020b). The pair of circulators may correspond to the circulator (1260) described with reference to FIG. 7. For example, the first circulator (1020a) may control the temperature value of bio-ink stored in the internal space of the first monitoring module (1030a) by circulating fluid into the multi-layer space included in the first monitoring module (1030a). Similarly, the second circulator (1020b) may control the temperature value of bio-ink stored in the internal space of the second monitoring module (1030b) by circulating fluid into the multi-layer space included in the second monitoring module (1030b).

[0196] The manufacturing module (1000b) may include a pair of monitoring modules. The manufacturing module (1000b) may include a first monitoring module (1030a) and a second monitoring module (1030b). The pair of monitoring modules may correspond to the monitoring module (1200) described with reference to FIG. 7.

[0197] The manufacturing module (1000b) may include a pair of extrusion modules. The manufacturing module (1000b) may include a first extrusion module (1040a) and a second extrusion module (1040b). The pair of extrusion modules may correspond to the extrusion module (1300) described with reference to FIG. 7. At least a portion of the bioink stored in the pair of monitoring modules may be transferred to the pair of extrusion modules by a pump. Thereafter, the pair of extrusion modules may extrude at least a portion of the bioink stored in the internal space to extract it in the form of a fiber bundle.

[0198] The manufacturing module (1000b) may include a pair of post-processing modules. The manufacturing module (1000b) may include a first post-processing module (1050a) and a second post-processing module (1050b). The pair of post-processing modules may correspond to the post-processing module (1400) described with reference to FIG. 7. The bioink extracted by the pair of extrusion modules described above may be introduced into the pair of post-processing modules, and a cross-linking reaction may occur by a cross-linking solution included in the pair of post-processing modules. The pair of post-processing modules may be formed to be detachably coupled to the manufacturing module (1000b).

[0199]

[0200] FIGS. 23 to 25 are drawings for explaining a monitoring module included in an expandable manufacturing module according to one embodiment.

[0201] Referring to FIGS. 23 and 24, a monitoring module (1030) according to one embodiment may include an outer housing (1031) and an inner housing (1032). The monitoring module (1030), like the monitoring module (1200) of FIG. 7, may have a double jacket structure and may have a multi-layer space (ml) formed by the outer housing (1031) and the inner housing (1032).

[0202] The internal housing (1032) may be provided with an internal space capable of accommodating bio-ink. The multi-layer space (ml) may be provided with a space through which fluid may circulate, and by circulating the fluid through the multi-layer space (ml) by the circulator (1020), the temperature of the bio-ink stored in the internal housing (1032) may be maintained at a constant level.

[0203] The monitoring module (1030) may include a window (1036) that allows the status of the bioink to be visually observed. The window (1036) may be formed in an elongated shape in the longitudinal direction. The window (1036) may be formed on the outside of the inner housing (1032), and a user may visually observe the status of the bioink contained in the inner housing (1032) (e.g., the type of bioink and the remaining amount of bioink) through the window (1036). The window (1036) may be made of a transparent polycarbonate material.

[0204] Referring to FIGS. 24 and 25, the monitoring module (1030) may include a blade (1033). The blade (1033) may have a configuration corresponding to the blade (1230) described with reference to FIG. 7, and any redundant description will be omitted.

[0205] The blade (1033) may be positioned so as to be spaced apart from the inner housing (1032) by a predetermined distance (d1). For example, the predetermined distance (d1) may be 10 mm. When the gap between the blade (1033) and the inner housing (1032) is formed within the predetermined distance, the bioink can be stirred more efficiently.

[0206] The blade (1033) can rotate by receiving power from the driving device (1034). At least one sensor can be placed on the driving device (1034) or the upper plate (1035), and the monitoring module (1030) can monitor the temperature or pH of the bio-ink contained in the internal housing (1032) based on the value measured from the at least one sensor.

[0207] The internal space of the monitoring module (1030) can be opened and closed by a separate device (e.g., a lifting device). The upper plate (1035) of the monitoring module (1030) can be moved up and down by a separate device, thereby opening and closing the internal space.

[0208] A cleaning device may be placed in the internal space of the internal housing (1032) of the monitoring module (1030). The cleaning device may be of various known types, and the operation of the cleaning device may result in cleaning of the internal space of the internal housing (1032).

[0209]

[0210] FIG. 26 is a diagram illustrating a method for controlling an extrusion module based on user input according to one embodiment. Referring to FIG. 26, a manufacturing device (100) according to one embodiment can receive user input from a user terminal (300) and control a first extrusion module (110) and a second extrusion module (120) based on the user input.

[0211] The user terminal (300) can obtain manufacturing information for artificial meat or artificial organs from the user via an interface. The artificial meat manufacturing information may be information regarding the first and second components that constitute the artificial meat. The first component may be protein, and the second component may be fat. The artificial meat manufacturing information may be information regarding the ratio of the first and second components.

[0212] The manufacturing device (100) can obtain artificial meat manufacturing information obtained from an external device (e.g., a user terminal) and control the first extrusion module (110) and the second extrusion module (120) based on the information. The first extrusion module (110) may be a module for extracting bio-ink (first bio-ink) regarding the first component, and the second extrusion module (120) may be a module for extracting bio-ink (second bio-ink) regarding the second component.

[0213] The manufacturing device (100) can determine parameters for extracting the first bio-ink and the second bio-ink based on the artificial meat manufacturing information. The manufacturing device (100) can determine at least one of the time point at which the first extrusion module (110) starts extruding the first bio-ink, the time point at which the extrusion ends, the extrusion speed, and the extrusion amount based on the artificial meat manufacturing information. The manufacturing device (100) can determine at least one of the time point at which the second extrusion module (120) starts extruding the second bio-ink, the time point at which the extrusion ends, the extrusion speed, and the extrusion amount based on the artificial meat manufacturing information.

[0214] The manufacturing device (100) can monitor and control the status of the first bio-ink and the second bio-ink based on the artificial meat manufacturing information. For example, the manufacturing device (100) can monitor and control the temperature and pH value of the first bio-ink so that they meet predetermined conditions based on the artificial meat manufacturing information, and can monitor and control the temperature and pH value of the second bio-ink so that they meet predetermined conditions.

[0215] The manufacturing device (100) can determine information regarding the pattern of artificial meat to be produced based on artificial meat manufacturing information. After obtaining information regarding the artificial meat pattern, the manufacturing device (100) can determine parameters for extracting the first bio-ink and the second bio-ink based on the information regarding the artificial meat pattern. The manufacturing device (100) can control the extrusion module in parallel based on the parameters determined based on the information regarding the artificial meat pattern, thereby producing artificial meat having a pattern desired by the user.

[0216] The configuration and operation method of the first extrusion module (110) and the second extrusion module (120) are the same as or corresponding to the contents described above with reference to FIGS. 10 to 20, so redundant descriptions will be omitted.

[0217]

[0218] FIG. 27 is a drawing for explaining an extrusion module according to another embodiment. Referring to FIG. 27, the extrusion module (1300) may be a device that applies pressure to bio-ink to extract it in the form of a plurality of fiber bundles. Since the configuration related to the extrusion device included in the drawing has been described above, a redundant description will be omitted. The present invention discloses a system for controlling the flow of a mixture, and herein, the term 'bio-ink' used throughout the specification does not refer to a specific single component, but can be interpreted as a combination of various substances, i.e., a mixture, including high viscosity fluids, bio-materials, edible materials, organic mixtures, inorganic mixtures, gels, powder formulations, and doughs. This indicates that the technical idea of ​​the present invention is not limited to a specific substance, but includes technical features for efficiently transporting and controlling various types of mixtures within the category of bio-ink, etc. Although the term 'bio-ink' is used herein, bio-ink can be interpreted interchangeably as various types of substances included in the above-described mixture.

[0219] At least a portion of the bio-ink stored in the hopper (1340) provided in the extrusion module (1300) can be moved by an external force into the internal space of the syringe (1310).

[0220] The extrusion module (1300) may include a hopper (1340). The hopper (1340) may have an internal space capable of containing bioink. The hopper (1340) may be fluidly connected to at least a portion of a transport device (1350). At least a portion of the bioink stored in the hopper (1340) may be fed into the transport device (1350) by an external force or gravity.

[0221] The extrusion module (1300) may include a transfer device (1350). The transfer device (1350) may control the flow of bioink. The transfer device (1350) may be a device that transfers bioink contained in the hopper (1340) or the monitoring module (1200) to the syringe (1310) and provides power to enable extrusion. The transfer device (1350) provides a mechanism for efficiently and stably transferring a fluid such as bioink, and may be designed to accommodate various material properties, such as high-viscosity or low-viscosity bioink. The transfer device (1350) may play a significant role in enhancing the accuracy and productivity of bioprinting or related manufacturing processes through precise fluid transfer.

[0222] The transport device (1350) can be implemented as one of various types of pumps, such as a piston pump that transports fluid through reciprocating motion. This allows for stable fluid transfer at a constant pressure and speed, while minimizing losses or irregularities that may occur during the fluid transport process. The transport device (1350) is designed to ensure stable transport while maintaining the properties of the bioink, making it advantageous for precision manufacturing processes such as bioprinting.

[0223] The transport device (1350) may include an extrusion device, which may include the syringe (1310), plunger (1320) and nozzle (1330) described above.

[0224] The extrusion module (1300) may include a mixer (1360). The mixer (1360) is a device that mixes various materials to create a homogeneous state. It may be a device used to create a homogeneous mixture by mixing different materials, such as powder and water. The mixer (1360) may include a rotating blade or a stirring device that mixes the materials internally, and may be designed to handle both high-viscosity and low-viscosity materials. The mixer (1360) may be designed to maintain physical or chemical properties while the materials are mixed.

[0225] Meanwhile, the extrusion module (1300) may also include a homogenizer or crusher. The homogenizer can perform the function of uniformly dispersing high-viscosity mixtures or fine particles, and the crusher can be utilized to crush solid materials to increase mixing efficiency. The above-described mixer (1360), homogenizer, and crusher are not essential components of the extrusion module (1300) and may be used optionally.

[0226]

[0227] Fig. 28 is a drawing for explaining a transport device according to one embodiment. Referring to Fig. 28, a transport device (1350) according to one embodiment may include an inlet (1351) through which bioink is introduced, an outlet (1352) through which bioink is discharged, a motor (1353) that provides power to a piston, a first fluid channel (1354), a second fluid channel (1355), a piston (1356) that reciprocates within a cylinder, and a check valve (1357) located between the inlet and the outlet and controlling the flow of fluid.

[0228] The inlet (1351) may be an inlet through which bioink flows in, and the outlet (1352) may be an outlet through which bioink is discharged.

[0229] The inlet (1351) can be fluidly connected to the hopper (1340), and at least a portion of the bio-ink contained in the hopper (1340) can be supplied to the transport device (1350) through the inlet (1351). The inlet (1351) can be formed in the direction of gravity, and at least a portion of the bio-ink contained in the hopper (1340) can be introduced into the inlet (1351) by gravity.

[0230] The outlet (1352) may be fluidly connected to the extrusion device, and at least a portion of the bio-ink present in the cylinder may be discharged into the extrusion device through the outlet (1352) by the reciprocating motion of the piston (1356). The outlet (1352) may be formed in a direction different from the direction of the inlet (1351). The outlet (1352) may be formed in a direction perpendicular to the direction of the inlet (1351).

[0231] The motor (1353) is a driving device and can provide the power required to operate the piston pump. The motor (1353) generates a rotational motion, and the rotational motion is converted into a reciprocating motion of the piston pump to assist in the stable transport of a fluid such as bioink. Various known technologies can be applied to the motor (1353), and for example, a brushless DC motor (BLDC) can be applied. In this case, it provides high precision and energy efficiency, and has low noise and vibration, which can be advantageous for the transport of sensitive materials such as bioink. The motor (1353) can be linked to a control system (e.g., at least one processor) and can operate in response to conditions such as the viscosity, flow rate, and pressure of the fluid.

[0232] The first fluid channel (1354) and the second fluid channel (1355) can provide a path for bioink to flow. The first fluid channel (1354) and the second fluid channel (1355) can serve as fluid passages for bioink to pass through and can be formed in a cylindrical structure. The first fluid channel (1354) can be formed in connection with the inlet (1351), and the second fluid channel (1355) can be formed in connection with the outlet (1352).

[0233]

[0234] FIG. 29 is a drawing for explaining the flow of fluid within a transport device according to one embodiment.

[0235] Referring to FIG. 29, when bioink is injected into the inlet (1351) of the transport device (1350) according to one embodiment, the bioink can be transferred through the first fluid channel (1354) to a piston (1356) that reciprocates within the cylinder. The bioink can be moved to the second fluid channel (1355) by the piston (1356) and then discharged through the outlet (1352).

[0236] The first fluid channel (1354) may include a first check valve (1357a), and the second fluid channel (1355) may include a second check valve (1357b). The check valves (1357a, 1357b) may perform a function of controlling the flow direction of the fluid and preventing backflow.

[0237] The first check valve (1357a) may be positioned adjacent to the inlet (1351) and may prevent the bioink from flowing backward when it is transferred from the inlet (1351) to the piston (1356) through the first fluid channel (1354). The second check valve (1357b) may be positioned adjacent to the outlet (1352) and may prevent the bioink from flowing backward when the piston (1356) pushes the bioink into the second fluid channel (1355) and may allow the bioink to be discharged to the outside through the outlet (1352).

[0238] The transport device (1350) may further include at least one processor, which may control the operation of the piston (1356) by executing at least one instruction stored in a memory.

[0239] The above processor can control the operating conditions of the piston (1356). Here, the operating conditions can be related to at least one of the reciprocating speed of the piston, the reciprocating cycle, the pressure, and the RPM value of the motor.

[0240] The processor can control the operation of the piston (1356) in response to the state of the extrusion device. The processor can control the operation of the piston according to the amount of bioink in the syringe (1310) of the extrusion device. For example, the processor can control the piston (1356) to reciprocate when the bioink is contained in the syringe (1310) of the extrusion device below a predetermined amount. Conversely, the processor can control the operation of the piston (1356) to stop when the bioink is contained in the syringe (1310) of the extrusion device above a predetermined amount.

[0241] The processor can control the operation of the piston (1356) based on the state of the bioink. The processor can control the operation of the piston (1356) under different conditions based on the viscosity of the bioink. The processor can determine the state of the bioink as one of low viscosity, medium viscosity, and high viscosity, and control the operation of the piston (1356) based on the determination. If the state of the bioink is determined to be low viscosity, the processor can control the piston at a first speed, if the state of the bioink is determined to be medium viscosity, the processor can control the piston at a second speed, and if the state of the bioink is determined to be high viscosity, the processor can control the piston at a third speed.

[0242] For example, the first speed may be faster than the second speed, and the second speed may be faster than the third speed. As a more specific example, the processor may provide a high flow rate by operating the piston reciprocating speed at a high speed when the bioink has low viscosity, provide a uniform flow by operating the piston reciprocating speed at an intermediate speed when the bioink has medium viscosity, and control the bioink to move at a stable and low speed by reducing the piston reciprocating speed when the bioink has high viscosity. As another example, the first speed may be slower than the second speed, and the second speed may be slower than the third speed.

[0243]

[0244] FIGS. 30 and 31 are drawings for explaining the transport efficiency when using a transport device according to one embodiment.

[0245] An experiment was conducted to evaluate the efficiency of transporting bioinks of various viscosities using a transport device (1350) according to one embodiment, and to verify the performance of the transport device (1350) of the present invention by comparing it with other types of pumps known in the art (e.g., a Longer Pump and an Air Diaphragm Pump).

[0246] For this experiment, bioinks were classified into low, medium, and high viscosity based on viscosity. Here, low-viscosity bioink was prepared with a composition of 2% alginate and 98% water, medium-viscosity bioink was prepared with a composition of 2% alginate, 1% methylcellulose (MC), 2% fish collagen, 15% palm oil, and 80% water, and high-viscosity bioink was prepared with a composition of 2% alginate, 1.5% methylcellulose (MC), 1% ISP, 0.5% salt, 0.5% sugar, 36% sunflower oil, and 58.5% water.

[0247] In this experiment, a Longer Pump, an Air Diaphragm Pump, and a transfer device (1350) were used. After measuring the initial weight of the empty container, bioink was transferred for 3 minutes using each pump, and the weight of the container after transfer was measured to calculate the weight of the transferred bioink. A total of three repeated experiments were performed, and the average transfer amount and efficiency were compared after repeated experiments.

[0248] Figure 30 (a) shows the results of comparing the transport efficiency of low-viscosity bioinks. Referring to Figure 30 (a), the average transport amount of the Longer Pump was 222.3 g, the average transport amount of the Air Diaphragm Pump was 675.0 g, and the average transport amount of the transport device (1350) was 5200.0 g. Consequently, the transport device (1350) of the present invention exhibited a transport efficiency that was approximately 23 times higher than that of the Longer Pump and approximately 7.7 times higher than that of the Air Diaphragm Pump in low-viscosity bioink.

[0249] Figure 30 (b) shows the results of comparing the transport efficiency of medium viscosity bioink. Referring to Figure 30 (b), the average transport amount of the Longer Pump was 77.3 g, the average transport amount of the Air Diaphragm Pump was 73.5 g, and the average transport amount of the transport device (1350) was 4466.0 g. Consequently, the transport device (1350) of the present invention showed a transport efficiency that was approximately 60 times higher, although the efficiency of the Longer Pump and the Air Diaphragm Pump did not differ significantly in the case of medium viscosity bioink.

[0250] Meanwhile, in terms of transport efficiency of high-viscosity bioink, the Longer Pump and the Air Diaphragm Pump were unable to transport high-viscosity bioink. In contrast, the transport device (1350) of the present invention was able to transport high-viscosity bioink, and the average transport amount was approximately 3916.7 g.

[0251] Figure 31 is a graph comparing the transfer efficiency by viscosity between each device. Referring to Figure 31, it can be confirmed that the transfer efficiency of all devices decreases as the viscosity increases. Specifically, in the case of low-viscosity bioink, the transfer device (1350), air diaphragm pump, and longer pump showed high efficiency in that order, and in the case of medium viscosity or higher, the transfer device (1350) showed the highest efficiency, and there was no significant difference in the efficiency of the air diaphragm pump and longer pump. In particular, in the case of high-viscosity bioink, the air diaphragm pump and longer pump did not operate, and only the transfer device (1350) was able to operate.

[0252] Through these experimental results, it can be seen that the transport device (1350) of the present invention is most effective in efficiently transporting bioinks of various viscosities.

[0253]

[0254] Fig. 32 is a drawing for explaining a manufacturing device according to another embodiment.

[0255] Referring to FIG. 32, a manufacturing device (500) according to another embodiment may include a processor (510), a hopper (520), a transport device (530), an extrusion device (540), a container (550), and a power supply device (560). The manufacturing device (500) may be designed to have a movable structure and may further include a driving unit for movement.

[0256] The processor (510) is a control device of the manufacturing device (500) and can control the operation of the transport device (530) and the extrusion device (540) in real time. The processor (510) can analyze process conditions such as the viscosity, extrusion speed, and flow rate of the bio-ink and control them to maintain an optimal operating state, and can precisely control the operation of the device according to conditions set by the user.

[0257] The hopper (520) can serve to store and supply bioink to be used in the manufacturing process. It can be designed to maintain the bioink in a stable state and can accommodate both high- and low-viscosity bioinks.

[0258] The transport device (530) can perform the function of stably transporting bioink from the hopper (520) to the extrusion device (540). It employs a piston-based design to maintain a constant flow rate and pressure regardless of the viscosity characteristics of the bioink, and includes a check valve to prevent backflow of the fluid. This is identical to the description described above with reference to FIGS. 5 to 8, and thus, a duplicate description will be omitted.

[0259] The extrusion device (540) may serve to extrude and extract bioink supplied through the transport device (530). It is designed to enable the bioink to be discharged in a shape or location required for final use, and provides precise extrusion control.

[0260] The container (550) may be a space for storing or temporarily storing bio-ink extracted from the extrusion device (540), and may be designed with a material that does not damage the bio-ink, and may be designed to facilitate further processing or storage after transport depending on the intended use.

[0261] The manufacturing device (500) may be designed as a movable structure and may thus include a power supply device (560) therein. The power supply device (560) may supply stable power to all components of the device and support the manufacturing device (500) to operate in various working environments as well as in a fixed location.

[0262]

[0263] Fig. 33 is a drawing for explaining a cell mixing device according to one embodiment. Referring to Fig. 33, an extrusion material production device (300) according to one embodiment may include a cell mixing device (Cell Mixer).

[0264] The cell mixing device (3000) is a device used to obtain an extruded material, and can serve to optimize the quality of the extruded material by uniformly mixing cells and at least one substance. The cell mixing device (3000) can perform the function of adjusting the physical and / or chemical properties of the composition so that a specific tissue structure can be formed, enabling uniform distribution of cells within the extruded material. The viscosity and fluidity can be adjusted during the mixing process using the cell mixing device (3000) to assist in the smooth extrusion process, and the stirring speed and mixing method can be precisely controlled so that physical damage is not inflicted on the cells.

[0265] The cell mixing device (3000) may be provided within the manufacturing device (100). An extruded material is manufactured through the cell mixing device (3000) provided within the manufacturing device (100), and the manufactured material can be processed into artificial meat.

[0266]

[0267] FIG. 34 is a drawing for explaining the configuration of a cell mixing device according to one embodiment. Referring to FIG. 34, a cell mixing device (3000) according to one embodiment may include a first stirring module (3100), a second stirring module (3200), and a third stirring module (3300).

[0268] The first stirring module (3100) and the second stirring module (3200) may be primary stirring modules, and the third stirring module (3300) may be a secondary stirring module. The primary stirring module may be a device for producing a primary pre-extrusion material, and the secondary stirring module may be a device for producing an extrusion material.

[0269] More specifically, the first stirring module may be a device for obtaining a pre-extrusion material by mixing cells and a predetermined first material, and the second stirring module may be a device for obtaining an extrusion material by mixing the pre-extrusion material and a predetermined second material.

[0270] Although the drawing illustrates the cell mixing device (3000) as including three stirring modules, the invention is not limited thereto, and the cell mixing device (3000) may include a primary stirring module (e.g., a first stirring module) and a secondary stirring module (e.g., a third stirring module), or the cell mixing device (3000) may include three or more primary stirring modules and two or more secondary stirring modules.

[0271]

[0272] Fig. 35 is a drawing for explaining the detailed configuration of a stirring module according to one embodiment. Referring to Fig. 35, a first stirring module (3100) may include a first tank (3110), a cell input tube (3120), a first material input tube (3130), a first stirring means (3140), and a first valve (3150).

[0273] The first stirring module (3100) may include a first tank (3110) having an internal space capable of accommodating a material. The first tank (3110) may include a cell inlet tube (3120) for injecting cells therein, a first material inlet tube (3130) for injecting a first material therein, a first stirring means (3140) for performing an operation of mixing the material, and a first valve (3150) for performing an operation of controlling the discharge of the material placed inside the tank.

[0274] The cell input tube (3120) is a passage that supplies cultured cells into the interior of the first tank (3110), and can be designed so that the cells are not damaged while maintaining a constant flow rate. The first material input tube (3130) can serve to inject biomaterials that can be mixed with cells, culture medium, PBS solution (phosphate buffered saline), saline solution, solutions having non-Newtonian fluid properties, alginate, or other additives into the interior of the first tank (3110), and the diameter and pressure of the tube can be adjusted according to the viscosity and flow properties of the material being input.

[0275] The first stirring means (3140) performs the function of uniformly mixing the introduced cells and the first material, and can be designed so that the stirring speed, rotation method (e.g., rotating blade, etc.), stirring intensity, etc. are controlled for efficient mixing.

[0276] The first valve (3150) plays a role in controlling opening and closing during the process of discharging the mixed material, and controls the discharge speed to enable a constant flow rate and a homogeneous mixture to be delivered to the subsequent process.

[0277] The third stirring module (3300) may include a third tank (3310) having an internal space capable of accommodating a material. The third tank (3310) may include a mixture inlet tube (3320) for injecting a material mixed in the first stirring module (3100) or the second stirring module (3200) into the tank, a second material inlet tube (3330) for injecting a second material into the tank, a third stirring means (3340) for performing an operation of mixing the material, and a third valve (3350) for performing an operation of controlling discharge of the material disposed inside the tank.

[0278] The mixture inlet tube (3320) is a passage that supplies the material (e.g., mixture) mixed in the first stirring module (3100) or the second stirring module (3200) into the third tank (3310), as described above, and may be designed to maintain a constant flow rate. The second material inlet tube (3320) may serve to inject a biomaterial, culture solution, PBS solution (phosphate buffered saline), saline solution, a solution having non-Newtonian fluid properties, alginate, or other additives that may be mixed with the mixture into the third tank (3310), and the second material may be the same or a different type of material as the first material.

[0279] The third stirring means (3340) performs the function of uniformly mixing the introduced mixture and the second material, and may be of a different type from the stirring means included in the first stirring module.

[0280]

[0281] FIGS. 36 and 37 are drawings for explaining the operation of a cell mixing device according to one embodiment.

[0282] Referring to FIG. 36, a cell mixing device (3000) according to one embodiment includes a step of introducing cells and a culture solution (or, PBS solution (phosphate buffered saline), saline solution, a solution having the characteristics of a non-Newtonian fluid, alginate or other additives, etc.) into a first tank and a second tank (S3110), a step of operating a first stirring means to stir the cells and the first material contained in the first tank to obtain a first mixture (S3120), a step of operating a second stirring means to stir the cells and the first material contained in the second tank to obtain a second mixture (S3130), a step of continuously introducing the second material into a third tank through a pump (S3140), a step of controlling a first valve to introduce the first mixture at a first time point (S3150), a step of controlling a second valve to introduce the second mixture into the third tank at a second time point (S3160), and a step of operating a third stirring means at a third time point to stir the solution contained in the third tank to obtain an extruded material. It can be operated through a step (S3170) and a step (S3180) of extruding the extruded material according to predetermined conditions. In this way, the cell mixing device (3000) can produce an extruded material by mixing cells and predetermined materials through a plurality of stirring modules.

[0283] Referring to FIG. 37, the cell mixing device (3000) can control the first stirring module (3100), the second stirring module (3200), and the third stirring module (3300) in parallel. The cell mixing device (3000) can sequentially introduce the mixture contained in the first stirring module (3100) and the second stirring module (3200) into the third stirring module (3300).

[0284] The cell mixing device (3000) may first input the mixture contained in the first stirring module (3100) into the third stirring module (3300), and after the operation is completed, may sequentially input the mixture contained in the second stirring module (3200) into the third stirring module (3300). The cell mixing device (3000) may sequentially input the mixture contained in the first stirring module (3100) and the mixture contained in the second stirring module (3200) into the third stirring module (3300) at different times.

[0285] The cell mixing device (3000) can control the first valve (3150) at a first time point to inject the first mixture into the third tank (3310), and can operate the third stirring means (3340) at a third time point to mix the material contained in the third tank (3310). Here, the third time point is after the first time point, and may be a time point at which it is confirmed that the first mixture has been completely injected into the third tank (3310).

[0286] For example, the first material may be a culture solution (or, PBS solution (phosphate buffered saline), saline solution, a solution having the properties of a non-Newtonian fluid, alginate, or other additives, etc.), and the first mixture may be a material in which cells and the first material are mixed. The second material may be an alginate solution, and the second mixture may be a material in which the first mixture and the alginate solution are mixed.

[0287] The first stirring means (3140) to the third stirring means (3340) can be implemented in various forms depending on the properties of the mixing target and the requirement for ensuring homogeneity of the mixing.

[0288] For example, it can be a stirring means by vibration, and it can operate in a way that induces flow within the mixed solution using vibration. Another example is an impeller-based stirring method, and it can be a method that forms a strong fluid flow using a rotating impeller blade, and it can be effective for mixing not only culture media but also high-molecular substances such as high-viscosity alginate solutions. The shape of the impeller can be various known shapes such as a Rushton type, a Marine type, a Turbine type, a Helical Impeller type, etc. In particular, when implemented in the form of a helical impeller, it is effective for stirring highly viscous substances due to its spiral structure, can induce a uniform flow, and can be more advantageous in increasing cell viability. Another example is a stirring means by a static mixer, and can cross-mix fluids in multiple stages through a fixed pattern structure inside. Another example is a stirring means that shakes the housing (tank) itself. For example, a stirring method may be applied that involves shaking the housing (tank) itself through linear reciprocating motion, rotational vibration, or gyroscopic mixing.

[0289] At least one of the first stirring means (3140) to the third stirring means (3340) may be applied with a mixing method using a 3-way stopcock valve. In the case of the 3-way stopcock valve, it may include a first inlet, a second inlet, an internal stirring unit, and an outlet, and cells and a first material may be introduced through the first inlet and the second inlet, materials introduced through the internal stirring unit are naturally mixed, and the mixed materials may be discharged through the outlet.

[0290]

[0291] FIGS. 38 and 39 are drawings for explaining the operation method of a plurality of cell mixing modules and an extrusion material composition module according to one embodiment.

[0292] Referring to FIG. 38, a cell mixing device (3000) according to one embodiment may operate through a step of generating and storing a first mixture using a first stirring means inside a first cell mixing module (S3210), a step of discharging the first mixture stored in the first cell mixing module to an extrusion material composition module (S3220), a step of operating the stirring means inside the extrusion material composition module when it is confirmed that the first mixture is input to the extrusion material composition module (S3230), a step of performing an operation of washing the inside of the first cell mixing module and / or an operation of generating an additional first mixture when it is confirmed that the discharge of the first mixture is complete (S3240), and a step of discharging a second mixture stored in a second cell mixing module to the extrusion material composition module when it is confirmed that the discharge of the first mixture is complete (S3250).

[0293] Referring to FIGS. 38 and 39, the first cell mixing module (CMM1) to the fifth cell mixing module (CMM5) may correspond to the first stirring module described above, and the material composition module (MFM) may correspond to the second stirring module described above.

[0294] The cell mixing device (3000) can control a plurality of cell mixing modules (CMMs) in parallel, and can sequentially control each cell mixing module (CMM). For example, the cell mixing device (3000) can first feed the mixture received in the first cell mixing module (CMM1) to the material composition module (MFM), and when the stirring operation in the material composition module (MFM) is completed, the mixture received in the second cell mixing module (CMM2) can be sequentially fed to the material composition module (MFM).

[0295] The cell mixing device (3000) can perform an operation of washing the interior of the first cell mixing module (CMM1) when it is confirmed that the first mixture has been discharged from the first cell mixing module (CMM1). The first cell mixing module (CMM1) can include a wastewater discharge unit, and wastewater generated after internal washing can be discharged to the outside through the wastewater discharge unit.

[0296] For example, the cell mixing device (3000) can monitor cells input into the first cell mixing module (CMM1) and determine whether to proceed with the internal cleaning operation of the first cell mixing module (CMM1) depending on the type of cells input into the first cell mixing module (CMM1). For example, after the cell mixing device (3000) confirms that the first cells have been input into the first cell mixing module (CMM1) and confirms that a mixture related to the first cells has been discharged, if it is determined that additionally input cells are different from the first cells, the cell mixing device (3000) can proceed with the internal cleaning operation of the first cell mixing module (CMM1).

[0297] As another example, the cell mixing device (3000) may include at least one of a residual cell detection sensor, a contaminant detection sensor, a conductivity sensor, and a pH sensor, and may determine whether cleaning of the interior of the first cell mixing module (CMM1) is required based on a value measured from the sensor.

[0298] When the cell mixing device (3000) confirms that the first mixture has been discharged from the first cell mixing module (CMM1), it can perform an operation of discharging the residue remaining inside the first cell mixing module (CMM1). For example, the operation of discharging the residue may be an operation of scraping the inside of the housing using a tool provided in the first cell mixing module (CMM1). As a more specific example, the operation of discharging the residue may be performed using a high-pressure air or cleaning agent spraying method, and may also be performed using a rotary scraper.

[0299] The cell mixing device (3000) can perform an operation of generating an additional first mixture by operating the first stirring means when it is confirmed that the first mixture has been completely discharged from the first cell mixing module (CMM1). The operation of generating the additional mixture can be performed by applying a pre-mixing method so that a continuous process flow is maintained in a manner in which the second mixture is pre-mixed in the second cell mixing module (CMM2) while the first mixture is discharged.

[0300]

[0301] FIG. 40 is a diagram illustrating a post-processing module according to one embodiment. Referring to FIG. 40, a post-processing module (1400) according to one embodiment may include a cross-linking module (1410), a washing module (1420), and a culture module (1430).

[0302] Fig. 41 is a drawing for explaining the configuration of a cross-linking module according to one embodiment. The cross-linking module (1410) may, as described above, secure the structural stability of the extruded material and perform a cross-linking reaction for final tissue formation. The cross-linking module (1410) may be implemented by utilizing a hydrogel-based material such as alginate, and cross-linking the extruded material through a reaction with a specific ion solution (e.g., CaCl2 solution) or other compound aqueous solutions (e.g., iron ion aqueous solution, copper ion aqueous solution, magnesium ion aqueous solution, polylysine), and an enzyme (transglutaminase).

[0303] Referring to FIG. 41, the bridge module (1410) may include a processor (1411), a first housing (1412), a second housing (1413), a third housing (1414), a peristaltic pump (1415), a circulation pump (1416), and a sensor (1417).

[0304] The processor (1411) controls the operation of components included in the crosslinking module (1410) in real time and can control the circulation system. The processor (1411) collects data from a sensor (1417) to form a feedback loop so that the crosslinking reaction can be performed in an optimal state, and can dynamically control the flow of the solution by controlling the operation of the pumps (1415, 1416) according to conditions.

[0305] The first housing (1412) may include an internal space capable of containing a specific ionic solution (e.g., a CaCl2 solution). A crosslinking reaction between the extruded material and the ionic solution may occur within the internal space.

[0306] The internal space of the first housing (1412) may include a stirring device. The stirring device may serve to induce the flow of the ion solution contained within the first housing (1412) and maintain a uniform distribution of the solution. Here, various known means such as a rotary impeller, a magnetic stirrer, or an ultrasonic stirrer may be utilized as the stirring method.

[0307] The internal space of the first housing (1412) may include a filter. The filter may function to prevent the extruded material from being sucked in by the pump (1415, 1416). For example, the filter may be a mesh filter, a porous membrane, a cyclone filter, etc. having a specific pore size.

[0308] The second housing (1413) may have an internal space, and an ion solution not yet used in a cross-linking reaction may be stored within the internal space. At least a portion of the ion solution contained within the second housing (1413) may be supplied to the first housing (1412) according to a predetermined standard. The second housing (1413) may be provided with a function to detect the solution concentration in real time by linking with a sensor (1417) and replenish fresh ion solution when the concentration falls below a standard, which may be controlled by a processor (1411).

[0309] The third housing (1414) may have an internal space, and purified water may be accommodated within the internal space. As the crosslinking reaction progresses, the solution within the first housing (1412) is gradually consumed, and when the amount of the solution decreases below a predetermined standard, at least a portion of the purified water stored in the third housing (1414) may be automatically supplied to the first housing (1412), which may be controlled by the processor (1411).

[0310]

[0311] FIG. 42 is a diagram illustrating a method for a processor to control the operation of a bridge module according to one embodiment.

[0312] Referring to FIG. 42, the processor (1411) may perform an operation of processing an extruded material through an extrusion system to create a fiber bundle (S1411), an operation of controlling the fiber bundle to move to a first housing of a cross-linking module (S1412), an operation of monitoring the concentration of an ionic solution (e.g., CaCl2) in the first housing through a conductivity sensor (S1413), an operation of controlling at least a portion of the solution in the second housing to be injected into the first housing when the concentration of the ionic solution is lower than a specific value (S1414), an operation of monitoring the capacity of the ionic solution in the first housing through a water level sensor (S1415), and an operation of controlling at least a portion of purified water in the third housing to be injected into the first housing when the capacity of the ionic solution is lower than a specific value (S1416).

[0313] The processor (1411) can control the fiber bundle to be positioned in a specific area within the first housing (1412). The internal space of the first housing (1412) can be divided into a plurality of areas, and the processor (1411) can determine any one of the plurality of areas as a target area based on predetermined criteria. The processor (1411) can control the extruded fiber bundle to be positioned in the target area. For example, the target area may be an area among the plurality of areas where the fiber bundle is not currently positioned.

[0314] The processor (1411) can measure the ion concentration of the solution in the first housing through a conductivity sensor, and based on this, control to supply at least a portion of the solution in the second housing (1413) into the first housing (1412). The processor (1411) can determine whether the ion concentration in the first housing is within a range appropriate for maintaining a cross-linking reaction using the conductivity sensor, and if it is determined based on the sensor value that the ion concentration needs to be diluted or supplemented, control to supply at least a portion of the solution in the second housing (1413) into the first housing (1412).

[0315] The processor (1411) can control the system to adjust the time of the crosslinking reaction or perform additional stirring operations based on the ion concentration within the first housing (1412).

[0316] The processor (1411) can monitor the remaining amount of the ion solution in the first housing (1412) through a level sensor or a weight sensor, and if it is determined that the ion solution has decreased below a certain threshold value, it can automatically perform a step of supplying purified water in the third housing (1414). If it is determined that the ion concentration in the first housing (1412) has been diluted to a reference value or more due to the supply of purified water in the third housing (1414), the processor (1411) can additionally supply the ion solution in the second housing (1413) into the first housing (1412).

[0317] Although not shown in the drawing, the cross-linking module (1410) may further include a fourth housing. The solution contained in the internal space of the fourth housing may be the same as or similar to the solution contained in the internal space of the first housing (1412). The processor (1411) may control at least a portion of the solution contained in the fourth housing to be supplied into the first housing when the concentration or volume of the solution contained in the first housing (1412) does not meet a predetermined standard based on a sensor value.

[0318]

[0319] Figure 43 is a drawing illustrating the configuration of a culture module according to one embodiment. The culture module (1430) may serve to culture an extruded material for which a crosslinking reaction has been completed for a predetermined period of time.

[0320] Referring to FIG. 43, the culture module (1430) may include a processor (1431), a first housing (1432), a second housing (1433), a third housing (1434), a peristaltic pump (1435), a circulation pump (1436), and a sensor (1437).

[0321] The processor (1431) can control the operations of all components included in the culture module (1430) in real time and automatically adjust the culture environment. The processor (1431) can collect data from a sensor (1437) to monitor the temperature, pH, nutrient concentration, etc. of the culture medium and form a feedback loop, and can automatically perform operations to replenish and replace the culture medium when necessary. The processor (1431) can control the operations of the pumps (1435, 1436) to optimize the flow of the culture medium and support the uniform culture of the extruded material in a constant environment.

[0322] The first housing (1432) can provide an internal space where culture can take place, and a culture medium mixture can be stored in the internal space. The extruded material can remain within the first housing (1432) for a certain period of time, allowing cells to grow and become organized.

[0323] The interior of the first housing (1432) can be controlled to maintain constant temperature, pH, dissolved oxygen, nutrient concentration, etc. The first housing (1432) can include a stirring device, and the stirring device can be controlled to uniformly distribute the culture mixture. The first housing (1432) can include a filter, and the extruded material being cultured can be prevented from being sucked by the pump (1435, 1436).

[0324] The first housing (1432) may include a holder. The holder may perform a function of controlling the extruded material so that it does not leave a specific area among a plurality of areas contained in the internal space of the first housing (1432).

[0325] The second housing (1433) may include an internal space for storing a fresh culture mixture, which may be used to replenish the culture mixture consumed during the culture process. The internal environment of the culture mixture within the second housing (1433) may be controlled to maintain a predetermined nutrient composition, pH, and temperature.

[0326] The second housing (1433) may contain a first type of culture element, and the third housing (1434) may contain a second type of culture element.

[0327] Alternatively, the third housing (1434) may include an internal space for storing purified water, and purified water may be replenished when the water level of the solution within the first housing (1432) decreases. For example, during the process of culturing within the first housing (1432), the concentration or remaining amount may change as the culture mixture evaporates or is consumed, and purified water may be additionally supplied to maintain this constant.

[0328] The above-described sensor (1417, 1437) may include multiple sensors, and may include, for example, a pH sensor, a temperature sensor, an oxygen sensor, a conductivity sensor, a water level sensor, a weight sensor, etc.

[0329]

[0330] FIG. 44 is a diagram illustrating a method for a processor to control the operation of a culture module according to one embodiment.

[0331] Referring to FIG. 44, the processor (1431) may perform a step of controlling a fiber bundle accommodated in a cross-linking module to move to a culture module (S1431), an operation of controlling the fiber bundle to be located in a specific area within the first housing using at least one holder (S1432), an operation of monitoring the pH and glucose concentration within the first housing through a sensor value (S1433), an operation of controlling the culture mixture within the first housing to be replaced with at least a portion of the culture mixture within the second housing through a circulation pump based on the monitoring result (S1434), an operation of monitoring the volume of the solution within the culture module through a water level sensor (S1435), and an operation of controlling at least a portion of the purified water within the third housing to be injected into the first housing when the volume of the solution within the first housing becomes lower than a specific value (S1436).

[0332] The processor (1431) can control the fiber bundle to be positioned in a specific area within the first housing (1432) using at least one holder. The internal space of the first housing (1432) can be divided into a plurality of areas, and the processor (1431) can determine any one of the plurality of areas as a target area based on predetermined criteria. The processor (1431) can control the fiber bundle to be cultured within the target area using the holder. In this way, the holder can perform a function of fixing the fiber bundle so that it does not leave the specific area within the first housing (1432).

[0333]

[0334] The processor (1431) can monitor pH, glucose concentration, etc. within the first housing through a sensor, and based on this, control to supply at least a portion of the culture mixture within the second housing (1433) into the first housing (1432). The processor (1431) can determine whether the culture mixture within the first housing is suitable for the culture environment using the sensor, and if it is determined that replacement or supplementation is necessary, control to supply at least a portion of the culture mixture within the second housing (1433) into the first housing (1432).

[0335] The processor (1431) can perform an operation of adjusting the culture time based on the monitoring result of the culture mixture within the first housing (1432).

[0336] The processor (1431) can monitor the remaining amount of the culture mixture in the first housing (1432) through a level sensor or a weight sensor, and if it is determined that the culture mixture has decreased below a certain threshold value, it can automatically perform a step of supplying purified water in the third housing (1434). If it is determined that the concentration of the culture mixture in the first housing (1432) has been diluted to a reference value or more due to the supply of purified water in the third housing (1434), the processor (1431) can additionally supply the culture mixture in the second housing (1433) into the first housing (1432).

[0337]

[0338] The processor (1411, 1431) can individually record the point in time when the extruded material is placed in a specific area of ​​the first housing (1412, 1432). The processor (1411, 1431) can divide the interior of the housing into multiple cross-linking areas or incubation areas, and measure and monitor the processing status of the material located in each area.

[0339] As the processor (1411, 1431) records the input time of each material, it can control the removal of the material or the performance of additional processing after a certain period of time has elapsed. For example, the appropriate cross-linking time and incubation time may vary depending on the type of material being cross-linked or cultured (e.g., fat fiber, protein fiber, etc.). The processor (1411, 1431) can set individual timers considering the optimal incubation / cross-linking time for each type of material, and when the set time for a specific material is exceeded, it can provide an alarm or automatically perform an operation to remove the material.

[0340] The processor (1411, 1431) may perform an operation to process materials that have exceeded their cross-linking or incubation time so that they can be discharged outside the housing through a removal module (e.g., an automatic transport device, a discharge valve, a robot arm, etc.). The processor (1411, 1431) may automatically detect materials that have exceeded a certain period of time at a specific location within the housing (1412, 1432) and perform an operation to discharge them.

[0341] The processor (1411, 1431) can provide an alarm to the user when the incubation and / or crosslinking time of the material is completed, and the interface can be configured to allow the user to manually confirm and remove it.

[0342] Meanwhile, the bridge module (1410) and the culture module (1430) may include a wastewater discharge unit, and waste materials generated during the circulation process of the solution may be discharged to the outside through the wastewater discharge unit.

[0343]

[0344] FIGS. 45 and 46 are drawings for explaining a method for a processor to control a post-processing process of an extruded material according to one embodiment.

[0345] Referring to FIG. 45, a processor (2000) according to one embodiment can control an artificial meat manufacturing automation system and selectively operate at least one of a plurality of processes included in the system depending on the type of extruded material.

[0346] The processor (2000) can identify the type of extruded material obtained from a plurality of extrusion modules (1300) and control the system so that different post-processing processes are performed based on the type of extruded material. For example, the post-processing processes may include a cross-linking operation performed by a cross-linking module (1410), a washing operation performed by a washing module (1420), and a culturing operation performed by a culturing module (1430), and the processor (2000) can control the system so that a post-processing process corresponding to the extruded material is performed.

[0347] Referring to FIG. 46, the processor (2000) can perform an operation (S2110) of determining the type of extruded material in a predetermined manner, an operation (S2120) of controlling to perform a first post-processing process if the extruded material is determined to be a first type, and an operation (S2130) of controlling to perform a second post-processing process if the extruded material is determined to be a second type.

[0348] For example, if the processor (2000) determines that the material obtained through the extrusion module (1300) contains cells, the processor (2000) may determine the material to be of the first type. The processor (2000) may control the system so that the material of the first type is post-processed according to a first post-processing process (e.g., a process in which a cross-linking operation, a washing operation, a culturing operation, and a washing operation are sequentially performed).

[0349] As another example, if the processor (2000) determines that the material obtained through the extrusion module (1300) does not contain cells, the processor (2000) may determine the material to be of the second type. The processor (2000) may control the system so that the material of the second type is post-processed according to a second post-processing process (e.g., a process in which a cross-linking operation and a washing operation are sequentially performed).

[0350]

[0351] FIG. 47 is a diagram illustrating a method for a processor to control and monitor a plurality of modules constituting an automation system according to one embodiment.

[0352] Referring to FIG. 47, an automated system according to one embodiment may include at least one extrusion module (1300), at least one crosslinking module (1410), at least one washing module (1420), at least one culture module (1430), and at least one assembly module (1500).

[0353] The processor (2000) can control multiple modules constituting the automated system based on control parameters. The processor (2000) can set control parameters by considering the type of extruded material (e.g., fat material, protein material, presence or absence of cells, etc.) and / or the extrusion timing, and can control multiple modules constituting the automated system based on these control parameters.

[0354] The automated system may include a plurality of extrusion modules (1300), and the processor (2000) may control the system so that a first post-processing process may be performed on the extruded material extracted from the plurality of extrusion modules (1300) by passing it through a cross-linking module (1410) and a washing module (1420).

[0355] The processor (2000) can control the system so that a second post-processing process is performed on the extruded material for which the first post-processing process has been completed according to predetermined criteria, or can control the system so that the material is processed through the assembly module (1500).

[0356] The culture module (1430) may include a plurality of containers. The plurality of containers may store a culture mixture, and the extruded material obtained through the plurality of extrusion modules may be fed into any one of the plurality of containers and cultured.

[0357]

[0358] FIGS. 48 and 49 are diagrams illustrating a method for a processor to manage the state of a culture module according to one embodiment.

[0359] Referring to FIGS. 48 and 49, the processor (2000) may perform an operation (S2210) of acquiring type information based on the type of solution contained in a plurality of containers included in a culture module, an operation (S2220) of acquiring operation information based on whether an extruded material is placed and cultured in the internal space of the plurality of containers, an operation (S2230) of acquiring culture time information based on the time at which the extruded material is placed in the plurality of containers in operation, an operation (S2240) of acquiring status information based on sensor values ​​for the plurality of containers, and an operation (S2250) of monitoring the culture module based on at least one of type information, operation information, culture time information, and status information.

[0360] The processor (2000) can obtain type information for the plurality of containers based on the composition of the solution contained in the plurality of containers (e.g., amino acid concentration, glucose concentration, temperature, type of additive, etc.). For example, if a specific type of cell culture is required, the processor (2000) can classify containers based on the concentration of a specific component of the culture mixture, and the processor (2000) can automatically adjust the culture protocol based on this.

[0361] The processor (2000) can determine whether culture is in progress by placing extruded materials in the internal spaces of multiple containers, and can obtain operational information about the multiple containers based on this. For example, the processor (2000) can automatically set containers in which culture is not in progress to a "non-operational state" by utilizing an optical sensor, an infrared sensor, or a weight detection sensor.

[0362] The processor (2000) can obtain incubation time information for each container based on the time at which the extruded material is placed in a plurality of containers in operation. The processor (2000) can record the time at which incubation starts in each container and obtain incubation time information based on this. The processor (2000) can obtain the incubation time information in real time by comparing the incubation start time with the current time, and when the target incubation time is reached, can provide an alarm to the user or control the module to perform a subsequent process.

[0363] The processor (2000) can obtain status information about a container based on sensor values ​​for multiple containers. For example, the processor (2000) can determine whether the culture environment is maintained normally through a pH sensor, a temperature sensor, a dissolved oxygen sensor, a conductivity sensor, a water level sensor, or a weight sensor.

[0364] The processor (2000) can monitor the culture module (1430) based on at least one of the type information, operation information, culture time information, and status information obtained by the above-described method.

[0365]

[0366] FIG. 50 is a diagram illustrating a method for a processor to control a plurality of containers included in a culture module according to one embodiment.

[0367] Referring to FIG. 50, a processor (2000) according to one embodiment may perform an operation (S2310) of determining the type of extruded material. The processor (2000) may determine the type of material based on physical and / or chemical properties of the extruded material. For example, the processor (2000) may determine the type of material based on protein-based material, fat-based material, mixed material, cell type, nutrient content, viscosity, etc.

[0368] The processor (2000) may perform an operation (S2320) of determining at least one candidate container corresponding to the type of the extruded material among a plurality of containers included in the culture module (1430). For example, the processor (2000) may determine the candidate container based on the composition of the culture medium inside the container, the temperature condition of the container, the availability status of the container, the oxygen concentration and pH level inside the container, etc.

[0369] The processor (2000) can perform an operation (S2330) of monitoring the status of a plurality of containers included in the culture module (1430) based on sensor values ​​(e.g., pH, temperature, dissolved oxygen concentration, etc.).

[0370] The processor (2000) may perform an operation (S2340) of determining one of the candidate containers as a target container based on at least one of the operation status and the status. The processor (2000) may preferentially select a container that is not in operation among the candidate containers, excluding a container currently being cultured, and may determine a container that is determined to have an optimal culture environment based on a sensor value as the target container.

[0371] The processor (2000) can perform an operation (S2350) of controlling the placement of extruded material in a predetermined area inside the target container and monitoring the incubation time. If it is confirmed that the extruded material has been incubated for a predetermined time based on the incubation time monitoring result, the processor (2000) can perform an operation (S2360) of controlling the extruded material placed in the target container to be discharged to the outside.

[0372]

[0373] FIGS. 51 and 52 are drawings illustrating an assembly module according to one embodiment.

[0374] Referring to FIGS. 51 and 52, a manufacturing device (100) of an artificial meat manufacturing system according to one embodiment may include a manufacturing module (1000), and the manufacturing module (1000) may include a first module (1100), a second module (1200), a third module (1300), a fourth module (1400), and a fifth module (1500). Since the first module (1100) to the fourth module (1400) have been described above with reference to FIG. 3, a redundant description thereof will be omitted.

[0375] The fifth module may be an assembly module (1500), and the assembly module (1500) may perform a function of forming a shape of artificial meat by assembling an extruded material (e.g., an extruded fiber bundle).

[0376] The assembly module (1500) can perform an operation of precisely arranging and combining extruded materials, thereby ultimately processing artificial meat having a tissue structure and appearance similar to actual meat. The assembly module (1500) includes a process of arranging extruded fiber bundles in a specific pattern and combining each fiber bundle. The combining method may include physical bonding, chemical bonding, mechanical compression, heat treatment, or a method utilizing a specific bio-adhesive.

[0377]

[0378] Fig. 53 is a drawing for explaining the configuration of an assembly module according to one embodiment. Referring to Fig. 53, an assembly module (1500) according to one embodiment may include an input unit (1510), a compression unit (1520), a first mold (1530), a second mold (1540), a plate (1550), and a post-processing unit (1560).

[0379] The above-mentioned input unit (1510) may perform a function of inputting an extruded material into a mold. For example, the input unit (1510) may perform a function of applying pressure to an extruded material contained in a syringe so that the extruded material fills the mold.

[0380] The above compression unit (1520) can perform a function of applying physical pressure to the extruded material inserted into the mold to ensure that the extruded material sufficiently fills the mold.

[0381] The first mold (1530) and the second mold (1540) may be placed on a plate (1550). The first mold (1530) and the second mold (1540) may provide a space (e.g., a groove) into which an extruded material may be filled, and may serve as a guide so that the extruded material may be formed into a certain shape.

[0382] The first mold (1530) and the second mold (1540) may include a plurality of grooves, and the plurality of grooves may include at least one groove having a different width. The first mold (1530) and the second mold (1540) may include a plurality of grooves whose widths are adjustable. The plurality of grooves may be formed so that their respective widths are adjustable according to a predetermined standard.

[0383] The first mold (1530) and the second mold (1540) may be provided in the form of interchangeable modules so that their shapes can be changed. When the form of the interchangeable modules is applied, artificial meat of various sizes and shapes can be produced through the first mold (1530) and the second mold (1540).

[0384] The first mold (1530) and the second mold (1540) may be used to process different types of materials. For example, the first mold (1530) may be used to assemble a first material, and the second mold (1540) may be used to assemble a second material.

[0385] The post-processing unit (1560) may perform a function of post-processing at least a portion of the extruded material filled in the first mold (1530) and the second mold (1540). For example, the post-processing unit (1560) may perform a function of cutting (or cutting out) at least a portion of the extruded material filled in the first mold (1530) and the second mold (1540). More specifically, when the input unit (1510) inputs the material in a first direction, the post-processing unit (1560) may perform a cutting operation in a second direction perpendicular to the first direction.

[0386]

[0387] FIGS. 54 to 56 are drawings for explaining a first method for manufacturing artificial meat by an assembly module according to one embodiment.

[0388] Referring to FIG. 54, an assembly module (1500) according to one embodiment can perform an operation of placing a first mold on a plate (S1511), an operation of filling a material into the first mold (S1512), an operation of removing the first mold and placing a second mold on the plate (S1513), an operation of filling a material into the second mold (S1514), an operation of removing the second mold and obtaining a unit structure (S1515), and an operation of manufacturing artificial meat using the unit structure (S1516).

[0389] The assembly module (1500) can perform an operation of placing a first mold (1530) on a plate (1550), as shown in (a) of FIG. 55.

[0390] The assembly module (1500) can perform an operation of filling an extruded material into a first mold (1530), as shown in (b) of FIG. 55. The assembly module (1500) can perform an operation of filling an extruded material into a groove of the first mold (1530) using an input unit (1510). For example, the assembly module (1500) can perform an operation of filling an extruded material into the first mold (1530) using an input unit (1510) that operates in an air cylinder manner.

[0391] The material filled in the groove of the first mold (1530) may be a first material unit (mu1), and the first material unit (mu1) may have a number and shape corresponding to the grooves included in the first mold (1530). For example, the first material unit (mu1) may be a material unit corresponding to fat.

[0392] The assembly module (1500) can perform an operation of removing the first mold (1530) and placing the second mold (1540) on the plate (1550), as shown in (c) of FIG. 55. The assembly module (1500) can place the first material unit (mu1) obtained through the first mold (1530) on the plate (1550) as is, and remove only the first mold (1530). The assembly module (1500) can place the second mold (1540) on the plate (1550) and the first material unit (mu1).

[0393] The assembly module (1500) can perform an operation of filling an extruded material into the second mold (1540), as shown in (d) of FIG. 55. Here, the extruded material filled through the second mold (1540) may be of a different type from the extruded material filled through the first mold (1530). The material may be filled into a groove of the second mold (1540). For example, the assembly module (1500) can perform an operation of filling an extruded material into the second mold (1540) through an input unit (1510) that operates in an air cylinder manner.

[0394] The material filled in the groove of the second mold (1540) may be a second material unit (mu2), and the second material unit (mu2) may have a number and shape corresponding to the grooves included in the second mold (1540). For example, the second material unit (mu2) may be a material unit corresponding to a protein.

[0395] The assembly module (1500) can perform an operation of removing the second mold (1540) and obtaining a unit structure (us), as shown in (e) and (f) of FIG. 55. The unit structure (us) can be a combination of at least one first material unit (mu1) and at least one second material unit (mu2).

[0396] Referring to Fig. 56, the unit structure (us) may be composed of at least one first material unit (mu1) and at least one second material unit (mu2) arranged adjacently on the same layer. The unit structure (us) may be composed in a form in which at least one first material unit (mu1) and at least one second material unit (mu2) are arranged in an intersecting manner.

[0397] The assembly module (1500) can perform an operation of manufacturing artificial meat using the unit structure (us). The assembly module (1500) can perform an operation of manufacturing artificial meat by applying a physical change to the unit structure (us). The operation of manufacturing artificial meat may be an operation of processing the unit structure (us) into a shape similar to actual meat.

[0398]

[0399] FIG. 57 is a drawing illustrating a second method for manufacturing artificial meat by an assembly module according to one embodiment.

[0400] Referring to FIG. 57, an assembly module (1500) according to one embodiment can perform an operation of placing a first mold on a plate and filling the first mold with a material (S1521), an operation of providing a physical change to the material placed on the plate (S1522), an operation of post-processing at least a portion of the material filled in the first mold (S1523), an operation of removing the first mold and placing a second mold on the plate (S1524), an operation of filling the second mold with a material (S1525), an operation of providing a physical change to the material placed on the plate (1526), ​​an operation of post-processing at least a portion of the material filled in the second mold (S1527), an operation of removing the second mold and obtaining a unit structure (S1528), and an operation of manufacturing artificial meat using the unit structure (S1529).

[0401] Some of the above operations (e.g., S1521, S1524, S1525, S1528, S1529) correspond to those described above through FIG. 54, so redundant descriptions are omitted.

[0402] The assembly module (1500) can perform an operation of providing a physical change to the material after filling the first mold (1530). The assembly module (1500) can apply a physical change (e.g., compression) to the material filled in the first mold (1530) through the compression unit (1520). By applying a physical change to the material filled in the first mold (1530), the material can be filled more densely in the groove of the first mold (1530). Similarly, the assembly module (1500) can perform an operation of providing a physical change to the material after filling the second mold (1540) with the material, which corresponds to the above-described content, and thus a duplicate description thereof will be omitted.

[0403] The assembly module (1500) can perform an operation (e.g., a cutting operation) for post-processing at least a portion of the material filled in the first mold (1530). The assembly module (1500) can obtain at least one first material unit (mu1) corresponding to a groove of the first mold (1530) by performing an operation (e.g., a cutting operation) for post-processing at least a portion of the material filled in the second mold (1540). Since this corresponds to the above-described content, a redundant description thereof will be omitted.

[0404]

[0405] FIG. 58 is a drawing illustrating how an assembly module according to one embodiment operates in multiple work areas.

[0406] Referring to FIG. 58, an assembly module (1500) according to one embodiment can obtain unit structures (us) from multiple work areas. The assembly module (1500) can be designed to obtain at least one first material unit (mu1) from a first work area (WA1) and at least one second material unit (mu2) from a second work area (WA2).

[0407] The assembly module (1500) can control the plate (1550) to sequentially move to the first working area (WA1) and the second working area (WA2). The assembly module (1500) can obtain at least one first material unit (mu1) and at least one second material unit (mu2) from different locations.

[0408] The assembly module (1500) may include a pair of input portions (1510). The pair of input portions (1510) may include a first input portion (1510a) and a second input portion (1510b), and the first input portion (1510a) may be disposed in the first work area (WA1), and the second input portion (1510b) may be disposed in the second input portion (1510b).

[0409] The first input unit (1510a) may be a device for inputting a first material, and the second input unit (1510b) may be a device for inputting a second material. The assembly module (1500) may obtain at least one first material unit (mu1) through the first input unit (1510a) in the first work area (WA1), and may obtain at least one second material unit (mu2) through the second input unit (1510b) in the second work area (WA2).

[0410]

[0411] FIGS. 59 to 61 are drawings for explaining a third method of manufacturing artificial meat by an assembly module according to one embodiment.

[0412] Referring to FIG. 59, an assembly module (1500) according to one embodiment can perform an operation of positioning a plate in a first working area (S1531), an operation of positioning a first mold on the plate (S1532), an operation of filling the first mold with a first material (S1533), an operation of removing the first mold and positioning a second mold on the plate (S1534), an operation of positioning the plate in a second working area (S1535), an operation of filling the second mold with a second material (S1536), an operation of removing the second mold and obtaining a unit structure (S1537), and an operation of manufacturing artificial meat using the unit structure (S1538).

[0413] The assembly module (1500) can perform an operation of positioning a plate (1550) in the first work area (WA1) and placing a first mold (1530) on the plate (1550), as shown in (a) of FIG. 60.

[0414] The assembly module (1500) can perform an operation of filling a first material into a first mold (1530) placed in a first working area (WA1), as shown in (b) and (c) of FIG. 60. The assembly module (1500) can perform an operation of filling the first material into the first mold (1530) through the first input portion (1510a). The assembly module (1500) can obtain at least one first material unit (mu1) through the operation of filling the first material into the first mold (1530).

[0415] Although not shown in the drawing, the assembly module (1500) can obtain at least one first material unit (mu1) through an operation of applying a physical change (e.g., compression) to the first material after filling the first mold (1530) with the first material and / or an operation of post-processing at least a portion of the first material (e.g., cutting).

[0416] The assembly module (1500) can perform an operation of removing the first mold (1530) and placing the second mold (1540) on the plate (1550), as shown in (d) of FIG. 60. The assembly module (1500) can perform an operation of positioning the plate (1550) in the second working area (WA2), as shown in (e) of FIG. 61. Although not shown in the drawing, the operation of placing the second mold (1540) on the plate (1550) can be performed after positioning the plate (1550) in the second working area (WA2).

[0417] The assembly module (1500) can perform an operation of filling a second material into a second mold (1540), as shown in (f) and (g) of FIG. 61. The assembly module (1500) can perform an operation of filling a second material into a second mold (1540) through a second input portion (1510b). The assembly module (1500) can obtain at least one second material unit (mu2) through the operation of filling a second material into a second mold (1540).

[0418] Although not shown in the drawing, the assembly module (1500) can obtain at least one second material unit (mu2) through an operation of applying a physical change (e.g., compression) to the second material after filling the second mold (1540) with the second material and / or an operation of post-processing at least a portion of the second material (e.g., cutting).

[0419] The assembly module (1500) can perform an operation of removing the second mold (1540) and obtaining a unit structure (us), as shown in (h) of FIG. 61. The assembly module (1500) can perform an operation of manufacturing artificial meat by processing the unit structure (us) based on a predetermined method.

[0420]

[0421] FIGS. 62 to 64 are drawings for explaining a method for manufacturing artificial meat having a predetermined pattern using an assembly module according to one embodiment.

[0422] Referring to FIG. 62, an assembly module (1500) according to one embodiment can perform a step (S1541) of obtaining a unit structure in which at least one first material unit and at least one second material unit are arranged adjacently on the same layer, and a step (S1542) of processing the unit structure to obtain artificial meat having a predetermined pattern in a cross-section.

[0423] The assembly module (1500) can obtain artificial meat by processing a unit structure in which at least one first material unit and at least one second material unit are arranged adjacently on the same layer. The assembly module (1500) can process the unit structure to produce artificial meat in which a user-desired pattern appears on the cross-section.

[0424]

[0425] Referring to FIGS. 63 and 64, an assembly module (1500) according to one embodiment may perform a step of obtaining a target pattern to be formed on an artificial meat cross-section from a user input (S1551), a step of determining a first condition related to a specification (length) of a unit structure based on the target pattern (S1552), a step of determining a second condition related to at least one of a type, number, size, and arrangement method of a first material unit and a second material unit constituting the unit structure based on the target pattern (S1553), and a step of determining a design condition based on the first condition and the second condition, and obtaining a unit structure based on the design condition (S1554).

[0426] The assembly module (1500) can acquire a target pattern to be formed on an artificial meat cross-section from user input or a predefined data set. The target pattern may include the muscle fiber arrangement, marbling (a combination of muscle and fat), tissue density, and directionality of actual meat, and may be a custom-designed pattern for a specific aesthetic or functional purpose.

[0427] The assembly module (1500) can determine a first condition related to at least one of the size, length, and width of the unit structure (us) based on the target pattern. The assembly module (1500) can determine a second condition related to at least one of the type, number, size, and arrangement method of at least one first material unit (mu1) and at least one second material unit (mu2) constituting the unit structure (us) based on the target pattern. The first condition and the second condition can be determined in consideration of the size of the artificial meat, the cross-sectional shape, the minimum size required to implement the target pattern, etc.

[0428] The assembly module (1500) can determine design conditions based on the first condition and / or the second condition, and obtain a unit structure (us) based on the design conditions.

[0429] The assembly module (1500) can manufacture artificial meat having a target pattern formed on a cross-section by applying a predetermined process (e.g., a rolling process, a cylindrical rolling process, a compressing process, etc.) to a unit structure (us) obtained by the above-described method.

[0430] The above unit structure (us) includes a plurality of first material units (mu1) and a plurality of second material units (mu2), and the plurality of first material units (mu1) and the plurality of second material units (mu2) can be arranged alternately (e.g., cross-arranged) on the same layer.

[0431] The assembly module (1500) can be controlled to adjust the width of the groove (slot) constituting the first mold (1530) and / or the second mold (1540) so as to satisfy the first condition and / or the second condition. Using the first mold (1530) and the second mold (1540) adjusted according to the first condition and the second condition, artificial meat having a target pattern formed on the cross-section can be obtained.

[0432]

[0433] FIGS. 65 and 66 are drawings for explaining a method for obtaining artificial meat by processing a unit structure including a plurality of layers by an assembly module according to one embodiment.

[0434] Referring to FIG. 65, an assembly module (1500) according to one embodiment may perform a step (S1561) of obtaining a first layer structure including at least one first material unit and at least one second material unit arranged adjacent to each other, a step (S1562) of obtaining a second layer structure including at least one first material unit and at least one second material unit arranged adjacent to each other, a step (S1563) of obtaining a unit structure composed of the first layer structure and the second layer structure, a step (S1564) of obtaining artificial meat by processing the unit structure through a pressing device having a predetermined shape, and a step (S1565) of obtaining artificial meat by processing the unit structure through a forming device including at least one roller. Here, the steps (S1564 and S1565) of obtaining artificial meat may be selectively performed.

[0435] Referring to FIG. 66, the assembly module (1500) obtains a unit structure (us), and the unit structure (us) may include a plurality of layer structures (e.g., a first layer structure (ls1) to a fourth layer structure (ls4)).

[0436] The first layer structure (ls1) may include at least one first material unit (mu1) and at least one second material unit (mu2), and the second layer structure (ls2) may include at least one first material unit (mu1) and at least one second material unit (mu2). The first layer structure (ls1) and the second layer structure (ls2) may be arranged vertically to form a unit structure (us). The first layer structure (ls1) and the second layer structure (ls2) may be assembled by being stacked vertically.

[0437] For example, multiple layer structures may have different properties. For example, the first layer structure (ls1) may be a high-protein fiber layer, the second layer structure (ls2) may be a fat layer, and the third layer (ls3) may be a low-density muscle layer. Such customized layering can artificially create a structure similar to a specific meat cut (e.g., sirloin, tenderloin, etc.).

[0438] The assembly module (1500) can manufacture artificial meat by processing a unit structure (us) composed of multiple layers obtained in this manner. The assembly module (1500) can obtain artificial meat by applying pressure to the unit structure (us) through a pressurizing device having a predetermined shape.

[0439] The assembly module (1500) can process the unit structure (us) through a molding device including at least one roller to obtain artificial meat. The molding device may include a pair of rollers (upper roller and lower roller). The molding device may include only the upper roller.

[0440] Artificial meat manufactured by processing a unit structure (us) including multiple layers can implement meat texture similar to actual meat through layer-by-layer arrangement of muscle fibers, and can improve strength and elasticity by increasing the bonding force between layers, and can form more precise marbling through appropriate arrangement of fat and protein layers.

[0441]

[0442] FIG. 67 is a drawing for explaining a cross-section of artificial meat manufactured by a manufacturing device according to one embodiment. Referring to FIG. 67, the manufacturing device (100) according to one embodiment can manufacture artificial meat having a pattern formed in the cross-section.

[0443] The above pattern may include, as described above, muscle fiber arrangement, marbling (mixture of muscle and fat), tissue density and directionality, and may be a custom designed pattern for a specific aesthetic or functional purpose.

[0444] The manufacturing device (100) can obtain artificial meat by processing the first material and the second material, and a pattern can be formed on the artificial meat by the first material and the second material.

[0445] The manufacturing device (100) can obtain a first microstructure (m1) by extruding a first material in a longitudinal direction, and can obtain a second microstructure (m2) by extruding a second material in a longitudinal direction. Here, the longitudinal direction may be a direction corresponding to the direction in which pressure is applied. More specifically, the manufacturing device (100) can obtain a first microstructure (m1) by processing a first material through an extrusion method using at least one nozzle, and can obtain a second microstructure (m2) by processing a second material.

[0446] The above pattern can be formed by at least one first microstructure (m1) obtained by processing a first material and at least one second microstructure (m2) obtained by processing a second material.

[0447] The above pattern can be formed by a combination (e.g., physical combination or chemical combination) of at least one first microstructure (m1) and at least one second microstructure (m2).

[0448] The above pattern can be formed by at least one first microstructure (m1) that serves to implement the organizational form of the pattern and at least one second microstructure (m2) that serves to form the background of the pattern.

[0449] The above pattern can be determined by at least one of the number of at least one first microstructure (m1), the arrangement (e.g., parallel arrangement, cross arrangement, random arrangement, etc.), and the bonding method (e.g., the method of assembling the microstructures, compressive strength, etc.).

[0450] The above pattern is formed by at least one first microstructure (m1), and the outer line of the area defined by the pattern can be formed to contact at least one second microstructure (M2). For example, the area defined by the pattern can be surrounded by a plurality of second microstructures (m2).

[0451] The manufacturing device (100) can obtain at least one first microstructure (m1) and at least one second microstructure (m2) by extruding a first material and a second material in the longitudinal direction in a first working space. The manufacturing device (100) can assemble at least one first microstructure (m1) and at least one second microstructure (m2) in a second working space through a physical and / or chemical bonding method. Here, the first working space and the second working space may be different spaces.

[0452] The manufacturing device (100) can obtain at least one first microstructure (m1) and at least one second microstructure (m2) by extruding the first material and the second material in the longitudinal direction at a first time point. The manufacturing device (100) can assemble the at least one first microstructure (m1) and the at least one second microstructure (m2) at a second time point through a physical and / or chemical bonding method. Here, the first time point and the second time point may be different time points.

[0453] For example, according to one embodiment, the manufacturing device (100) can obtain an artificial meat having a pattern formed on a cross-section through an assembly process of the microstructure after obtaining a microstructure through an extrusion method and then performing the post-processing process described above.

[0454]

[0455] The cross-section of the artificial meat manufactured by the manufacturing device (100) may include a plurality of patterns, and the plurality of patterns may have a certain repeatability and may be formed in different shapes.

[0456] At least one of the plurality of patterns included in the cross-section of the artificial meat may be formed with a minimum width of 1 mm to 2 mm or less. Regardless of the geometric shape of the plurality of patterns, at least one of the plurality of patterns may be formed with a minimum width (minimum cross-sectional dimension) constituting the pattern of 1 mm to 2 mm or less.

[0457] The plurality of patterns included in the cross-section of the artificial meat may have various geometric shapes, but the minimum width of an individual structural element constituting at least one of the plurality of patterns may be formed to be 1 mm to 2 mm or less.

[0458] The shape of the plurality of patterns included in the cross-section of the artificial meat has a plurality of measurable widths (e.g., a minimum width, a minimum cross-sectional dimension, a minimum characteristic dimension, any one of the geometric elements within the pattern, the width of the spatially narrowest part, etc.), and at least one of the plurality of measurable widths can be formed to be 1 mm to 2 mm or less.

[0459] For example, at least one of the plurality of patterns may be in a polygonal shape, and the plurality of measurable widths within the polygonal shape may be formed to be 1 mm to 2 mm or less. As another example, at least one of the plurality of patterns may be in a circular shape, and the minimum diameter of the circular shape may be formed to be 1 mm to 2 mm or less.

[0460]

[0461] The cross-section of the artificial meat manufactured by the manufacturing device (100) may include a plurality of pattern areas defined by a plurality of patterns. At least one of the plurality of pattern areas may be a minimum pattern area.

[0462] The minimum pattern area may be a pattern structure formed to have specific physical or visual characteristics within the artificial meat cross-section, and may have an area smaller than a certain size. The minimum pattern area may be an area defined by a pattern formed to a predetermined size or smaller. The minimum width of the pattern located within the minimum pattern area may be 1 mm to 2 mm or less.

[0463] The minimum pattern area may include a feature area formed by at least one first microstructure and a background area where an outer line of the feature area contacts at least one second microstructure.

[0464] The minimum pattern area can be defined by a predefined guide tool. The guide tool can identify patterns smaller than a certain size on the cross-section of the artificial meat and define these as the minimum pattern area. In other words, the guide tool filters patterns of a certain size, thereby determining patterns smaller than that size as the minimum pattern area.

[0465] Among the multiple patterns included in the artificial meat cross-section, a pattern that is distinguished by a guide tool to be smaller than a certain size can be defined as the minimum pattern area. The guide tool can set a projection area of ​​a certain size, and among the multiple patterns included in the artificial meat cross-section, a pattern detected within that area can be defined as the minimum pattern area. A pattern included within the projection area set by the guide tool can be defined as the minimum pattern area.

[0466] For example, the guide tool may be circular, and the diameter of the circle may be 1 mm to 2 mm or less. In this case, the cross-section of the artificial meat manufactured by the manufacturing device (100) may have at least one minimum pattern area included within the projection area set by the guide tool (i.e., an area having a diameter of 1 mm to 2 mm or less).

[0467]

[0468] The features, structures, effects, etc. described in the embodiments above are included in at least one embodiment of the present invention, and are not necessarily limited to just one embodiment. Furthermore, the features, structures, effects, etc. exemplified in each embodiment can be combined or modified in other embodiments by those skilled in the art to which the embodiments pertain. Therefore, the contents related to such combinations and modifications should be construed as falling within the scope of the present invention.

[0469] In addition, although the above description focuses on the embodiments, these are merely examples and do not limit the present invention. Those skilled in the art to which the present invention pertains will appreciate that various modifications and applications not exemplified above are possible without departing from the essential characteristics of the present embodiments. In other words, each component specifically shown in the embodiments can be modified and implemented. In addition, differences related to such modifications and applications should be interpreted as being included within the scope of the present invention defined in the appended claims.

Claims

1. In a device for producing artificial meat using bio-ink, multiple modules; and At least one processor controlling the plurality of modules; The above multiple modules are, Includes a monitoring module and an extrusion module, At least one processor, Controlling the state of the bio-ink to maintain a predetermined condition through the above monitoring module, Controlling that at least a portion of the bio-ink is extruded and extracted through the extrusion module; Artificial meat manufacturing device.

2. In paragraph 1, The monitoring module includes an internal space capable of accommodating the bio-ink and at least one sensor for monitoring the status of the bio-ink accommodated in the internal space. wherein said at least one sensor comprises a first sensor for measuring the temperature of said bioink or a second sensor for measuring the pH of said bioink, Artificial meat manufacturing device.

3. In paragraph 2, The above extrusion module further includes a nozzle having a plurality of holes, At least one processor, Controlling the extrusion module to allow at least a portion of the bio-ink to pass through the nozzle and be extracted in the form of a fiber bundle; Artificial meat manufacturing device.

4. In paragraph 3, Each of the plurality of holes provided in the nozzle is formed to have a predetermined identical diameter, is formed to be spaced apart from each other by a predetermined interval, and is formed to have a Y-shaped cross-section. Artificial meat manufacturing device.

5. In paragraph 4, The above artificial meat manufacturing device further includes a cross-linking module, The above-mentioned cross-linking module has an internal space capable of accommodating a cross-linking solution, At least one processor, Controlling the bio-ink extracted by extrusion through the extrusion module to be injected into the internal space of the cross-linking module. Artificial meat manufacturing device.

6. In paragraph 5, The bioink comprises a first bioink related to fat and a second bioink related to protein, The monitoring module includes a first monitoring module for monitoring the first bio-ink and a second monitoring module for monitoring the second bio-ink, The extrusion module includes a first extrusion module for extruding the first bio-ink and a second extrusion module for extruding the second bio-ink, The cross-linking module includes a first cross-linking module into which the first bio-ink is extracted and a second cross-linking module into which the second bio-ink is extracted. Artificial meat manufacturing device.

7. In paragraph 6, The above plurality of modules are composed of a first unit and a second unit, The first unit includes the first monitoring module, the first extrusion module, and the first crosslinking module, and the second unit includes the second monitoring module, the second extrusion module, and the second crosslinking module. The at least one processor controls the first unit and the second unit in parallel, Artificial meat manufacturing device.

8. In paragraph 5, The above-mentioned bridge module includes an electrical conductivity sensor, Based on the above electrical conductivity sensor, the contamination concentration of the cross-linking solution contained in the internal space of the cross-linking module is measured, If the above contamination concentration is judged to exceed a predetermined level, the cross-linking solution is controlled to be circulated and replaced. Artificial meat manufacturing device.

9. In paragraph 5, The above artificial meat production device further includes a storage module, The above storage module has an internal space capable of storing the bioink, At least one processor, Controlling so that at least a portion of the bio-ink contained in the internal space of the storage module can be moved to the monitoring module through the pump, Artificial meat manufacturing device.

10. A method for producing artificial meat using bioink, A step of storing bio-ink in the internal space of the storage module; A step of moving at least a portion of the bio-ink contained in the internal space of the storage module to the monitoring module through a pump; A step of controlling the state of the bio-ink stored in the above monitoring module to maintain a predetermined condition; and A step of controlling at least a portion of the bio-ink stored in the monitoring module to be extruded and extracted through the extrusion module; Method for producing artificial meat.

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