Test chip, test device, and test method

Abstract

A test chip, a test device, and a test method. The test chip comprises a sample loading slot, a separation unit, a flow diversion unit, at least one injection slot, at least one test slot, and at least one waste liquid unit. By using the test chip disclosed in the present invention, and only by means of adjusting a rotational direction and the magnitude of acceleration during centrifugation, a single chip can implement the functions of separation, reaction and cleaning of a sample to be tested, thereby conveniently and quickly completing the test of the sample. Moreover, a stepped slot contained in the waste liquid unit can further avoid the problem of waste liquid backflow.

Description

Detection chips, detection devices and detection methods

[0001] This application claims priority to Chinese patent application No. 202410922509.5, filed on July 10, 2024, the entire contents of which are incorporated herein by reference. Technical Field

[0002] This disclosure relates to the field of biological detection technology, and in particular to a detection chip, detection device and detection method. Background Technology

[0003] There are many types of biological tests. For example, bodily fluid samples such as plasma, urine, and saliva contain many substances that provide physiological or clinical information. By conducting biological tests on these samples, medical personnel or testing units can quickly understand an individual's physical condition.

[0004] As the sensitivity of detection methods continues to improve, the application scope of biological detection is rapidly expanding, the operational threshold is gradually decreasing, and its commercial value is becoming increasingly important. For example, enzyme-linked immunosorbent assay (ELISA) is also a commonly used biological detection technology.

[0005] However, to ensure the accuracy of the test results, the sample needs to undergo a series of operations such as centrifugation, reaction, and cleaning during the testing process. In existing technologies, the methods for detecting substances in samples generally involve first centrifuging with large centrifuge equipment, and then transferring the sample to a specialized testing instrument for reaction, cleaning, and detection.

[0006] Such segmented operations, besides increasing the risk of sample contamination, also require operators to have a high level of professional knowledge. Furthermore, with the development of microfluidic control technology, although existing detection chips can achieve sample separation, an integrated technology that can complete all detection processes on a single detection chip has not yet been developed. Summary of the Invention

[0007] To address the problems mentioned in related technologies, this disclosure provides a detection chip, a detection device, and a detection method. The detection chip includes a sample loading cell, a separation unit, a flow splitting unit, at least one detection cell, at least one injection cell, and at least one waste liquid unit.

[0008] The separation unit is connected to the sample loading tank, and the diversion unit is connected to the separation unit. The at least one detection tank is connected to the diversion unit, and the at least one injection tank is connected to at least one flow channel connecting the diversion unit and the at least one detection tank. The at least one waste liquid unit is connected to the at least one detection tank. Each of the at least one waste liquid unit includes a stepped tank.

[0009] Based on the aforementioned detection chip, the detection device described in this disclosure includes a driving mechanism. The detection chip is detachably connected to the driving mechanism.

[0010] Based on the aforementioned detection chip and detection device, this disclosure further provides a detection method for the detection chip.

[0011] First, in step (A), a sample to be tested is added into a sample loading tank.

[0012] Next, step (B) drives the detection chip to rotate and separates the sample to be tested from the sample loading cell into a detection sample through a separation unit.

[0013] Next, in step (C), the test sample is transported to the corresponding at least one test cell via the diversion unit, and in step (D), at least one reaction solution is added to the at least one test cell through at least one injection tank, so that the test sample and the at least one reaction solution are mixed and incubated to obtain a mixture containing at least one intermediate, and then a waste liquid in the mixture is removed by centrifugation.

[0014] Subsequently, step (E) involves adding a cleaning solution to the at least one detection tank through the at least one injection tank, thereby cleaning the at least one intermediate with the cleaning solution.

[0015] Finally, step (F) involves adding at least one substrate into the at least one detection cell through the at least one injection cell to initiate a reaction, and detecting the reaction result presented in the at least one detection cell.

[0016] The above brief description of this disclosure is intended to provide a basic explanation of several aspects and technical features of this disclosure. This brief description is not a detailed description of this disclosure, and therefore its purpose is not to specifically list the key or important elements of this disclosure, nor to define the scope of this disclosure, but only to present several concepts of this disclosure in a concise manner. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments or related technologies of this disclosure, the accompanying drawings used in the description of the embodiments or related technologies will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained based on the structure of these drawings without any effort.

[0018] Figure 1 is a schematic diagram of the structure of a detection chip according to some embodiments of the present disclosure.

[0019] Figure 2 is a schematic diagram of the structure of a separation unit of a detection chip according to some embodiments of the present disclosure.

[0020] Figure 3 is a schematic diagram of the structure of a current shunting unit of a detection chip according to some embodiments of the present disclosure.

[0021] Figure 4 is a schematic diagram of another shunt unit of a detection chip according to some embodiments.

[0022] Figure 5 is a schematic diagram of the structure of the injection groove according to some embodiments.

[0023] Figure 6 is a schematic diagram of the structure of the detection groove according to some embodiments.

[0024] Figure 7 is a schematic diagram of the structure of a waste liquid unit of a detection chip according to some embodiments.

[0025] Figure 8 is a structural schematic diagram of a waste liquid unit according to some embodiments.

[0026] Figure 9 is a schematic diagram of the structure of another detection chip according to some embodiments.

[0027] Figure 10 is a schematic diagram of the structure of another detection chip according to some embodiments.

[0028] Figure 11 is a schematic diagram of the structure of another detection chip according to some embodiments.

[0029] Figure 12 is a schematic diagram of the structure of a detection device according to some embodiments of the present disclosure.

[0030] Figure 13 is a schematic diagram of the detection device according to some embodiments.

[0031] Figure 14 is a flowchart of a detection method according to some embodiments of the present disclosure.

[0032] Figures 15A-15G are schematic diagrams of detection chip operation methods according to some embodiments of the present disclosure.

[0033] Figure Descriptions: 1-Detection chip, 10-Rotation center, 21-Sampling groove, 211-Sampling orifice, 22-Separation unit, 221 First flow channel, 222-Separation groove, 223-First microchannel, A1-First connection end, A2-Second connection end, A3-First bend end, 23-Diverting unit, 231-Diverting flow channel, 232-Quantitative groove, 233-Second microchannel, 234-Reservoir, 2341-First reservoir, 2342-Second reservoir, 235 - First microfluidic valve, 24-Injection tank, 240-Accommodation space, 241-Limiting threshold, 25-Detection tank, 251-First detection chamber, 252-Second detection chamber, 26-Waste liquid unit, 261-Third microfluidic channel, 262-Waste liquid tank, 263-Second microfluidic valve, 264-Exhaust port, 265-Stepped groove, B1-Third connecting end, B2-Second turning end, B3-Fourth connecting end, 3-Drive mechanism, 4-Magnetic mechanism, 41-Magnetic block, D-Detection device. Detailed Implementation

[0034] The technology in the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this disclosure, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this disclosure without any effort are within the scope of protection of this disclosure.

[0035] Therefore, this disclosure provides a detection chip in some embodiments. Please refer to FIG1, which is a schematic diagram of the structure of a detection chip according to some embodiments of this disclosure. As shown in FIG1, the detection chip 1 in FIG1 includes a sample loading tank 21, a separation unit 22, a diversion unit 23, at least one injection tank 24, at least one detection tank 25 and at least one waste liquid unit 26 arranged sequentially from the rotation center 10 to the outer periphery.

[0036] Figure 1 shows the separation unit 22 connected to the sample loading tank 21 in the embodiment. The separation unit 22 is used to separate the sample to be tested into groups that can be subsequently detected according to the density gradient, i.e., the test samples; as for the remaining samples, since they have different densities from the test samples, the samples of other densities are retained in the separation unit 22 as a separate group.

[0037] Next, the diversion unit 23 of this embodiment is connected to the separation unit 22. The diversion unit 23 is used to divert the test sample separated according to the density gradient into at least one test tank 25. At least one injection tank 24 is connected to the test tank 25, and the injection tank 24 is used to inject reaction solution, cleaning solution or substrate into the test tank 25.

[0038] Furthermore, in this embodiment, at least one detection slot 25 is connected to the diversion unit 23. For example, at least one detection slot 25 is connected to the diversion unit 23 via at least one flow channel (e.g., at least one second microchannel 233 hereinafter). The detection slot 25 is used to receive the diverted detection sample. In this embodiment, the following processes are mainly performed within the detection slot 25.

[0039] First, the sample reacts with the reaction solution injected through injection tank 24, and then is centrifuged to obtain an intermediate and impurities. Next, the intermediate is cleaned with a cleaning solution injected through injection tank 24 to remove impurities. Finally, the intermediate reacts with the substrate injected through injection tank 24 to complete the detection.

[0040] After the test is completed, at least one waste liquid unit 26 in this embodiment is connected to the test tank 25. The waste liquid unit 26 is mainly used to collect the waste liquid output from the test tank 25 in the above process.

[0041] Next, please refer to Figure 2, which is a schematic diagram of the structure of the separation unit of the detection chip according to some embodiments of the present disclosure. As shown in Figure 2, the separation unit 22 in the embodiment of Figure 2 includes a first flow channel 221, a separation groove 222, and a first microflow channel 223.

[0042] The first flow channel 221 is used to control the movement of the test sample from the sample loading tank 21 to the separation tank 222, and the separation tank 222 is used to separate the test sample into the test sample and samples of other densities according to the density gradient. Then, the first microflow channel 223 is used to control the movement of the test sample from the separation tank 222 to the splitting unit 23.

[0043] It is understood that in this embodiment, the first flow channel 221 is connected to the sample loading tank 21 and the separation tank 222 respectively. In this embodiment, the first microchannel 223 includes a first connecting end A1, a second connecting end A2, and a first turning end A3.

[0044] The first connecting end A1 is connected to the separation tank 222 and is located at the first radius. The second connecting end A2 is connected to the diversion unit 23 and is located at the second radius. The first turning end A3 is located between the first connecting end A1 and the second connecting end A2 and is located at the third radius.

[0045] It is understood that the third radius is smaller than the first radius, and the first radius is smaller than the second radius. Furthermore, the first radius, second radius, and third radius refer to the distances of the first connecting end A1, the second connecting end A2, and the first turning end A3 from the center (or center of mass) of the entire detection chip 1, respectively.

[0046] Please refer further to Figure 3, which is a schematic diagram of the structure of a current-splitting unit of a detection chip according to some embodiments of the present disclosure. As shown in Figure 3, the current-splitting unit 23 of this embodiment includes a current-splitting channel 231, at least a certain amount groove 232, at least a second microchannel 233, and a liquid storage tank 234.

[0047] The diversion channel 231 is used to receive the test sample delivered from the first microchannel 223. In the diversion channel 231, the test sample is first allocated to the quantitative tank 232, and the excess test sample is allocated to the storage tank 234.

[0048] In this embodiment, the quantitative tank 232 is used for quantitative pre-dispensing of the test sample, while the second microchannel 233 is used to control the movement of the test sample from the quantitative tank 232 to the test tank 25. It should be noted that in this embodiment, one end of the diversion channel 231 is connected to the second connecting end A2 of the first microchannel 223, and the other end is connected to the storage tank 234. At least one quantitative tank 232 is connected to the diversion channel 231 between the second connecting end A2 and the storage tank 234, and at least one second microchannel 233 is connected to the quantitative tank 232.

[0049] In some embodiments, referring to FIG4, which is a schematic structural diagram of another diversion unit according to some embodiments of the present disclosure, a first microfluidic valve 235 is provided on the second microfluidic channel 233 in some embodiments based on FIG4. The first microfluidic valve 235 can enhance the control force of the second microfluidic channel 233 on the movement of the detection sample from the quantitative groove 232 to the detection groove 25.

[0050] In some other embodiments based on Figure 4, the diversion channel 231 is spiral-shaped, and the angle between the liquid storage tank 234 at the end of the diversion channel 231 and the circumference of the diversion channel 231 is not less than 20°. This design of the diversion channel 231 facilitates the collection of residual test samples in the diversion channel 231 into the liquid storage tank 234 after the test sample has been diverted to at least one test tank 25.

[0051] Referring again to Figure 4, in some embodiments based on Figure 4, a first liquid storage chamber 2341 and a second liquid storage chamber 2342 are provided in the liquid storage tank 234. The depth difference between the first liquid storage chamber 2341 and the second liquid storage chamber 2342 is not less than 2 mm. This depth difference helps to maintain the liquid-gas interface line and prevent liquid backflow in the liquid storage tank 234.

[0052] Next, please refer to Figure 5, which is a schematic diagram of the structure of an injection tank according to some embodiments of the present disclosure. In some embodiments based on Figure 5, a limiting threshold 241 is provided in the injection tank 24, thereby forming a receiving space 240 within the injection tank 24. In this embodiment, a drying reagent or a lyophilized reagent can be pre-embedded in the receiving space 240 to reduce the risk of contamination when using liquid reagents in related technologies. When the drying reagent or lyophilized reagent needs to be used, in this embodiment, an activator is first injected into the injection tank 24 to activate the pre-embedded drying reagent or lyophilized reagent.

[0053] Referring again to Figure 6, which is a schematic diagram of the structure of a detection tank according to some embodiments of the present disclosure. In some embodiments disclosed in Figure 6, a first detection cavity 251 and a second detection cavity 252 are provided within the detection tank 25, and the depth difference between the first detection cavity 251 and the second detection cavity 252 is not less than 2 mm. This depth difference helps maintain the liquid-gas interface line, ensuring a consistent liquid level during detection, thereby improving the accuracy of the detection.

[0054] Please refer to Figure 7, which is a schematic diagram of the structure of a waste liquid unit of a detection chip according to some embodiments of the present disclosure. In some embodiments based on Figure 7, at least one waste liquid unit 26 includes a third microchannel 261 and a waste liquid tank 262. One end of the third microchannel 261 is connected to the detection tank 25, and the other end is connected to the waste liquid tank 262. In this embodiment, the third microchannel 261 is used to control the movement of waste liquid from the detection tank 25 to the waste liquid tank 262.

[0055] The third microchannel 261 in this embodiment includes a third connecting end B1, a second turning end B2, and a fourth connecting end B3 (see Figure 8). The third connecting end B1 is connected to the detection tank 25 and is located at the fourth radius. The fourth connecting end B3 is connected to the waste liquid tank 262 and is located at the fifth radius.

[0056] The second turning point B2 is located between the third connecting point A1 and the fourth connecting point A2, and is situated at the sixth radius. The sixth radius is smaller than the fourth radius, and the fourth radius is smaller than the fifth radius. Furthermore, the fourth, fifth, and sixth radii refer to the distances of the third connecting point B1, the fourth connecting point B3, and the second turning point B2 from the center (or center of mass) of the entire detection chip 1, respectively.

[0057] In some embodiments, please refer to FIG8, which is a schematic diagram of another waste liquid unit according to some embodiments of the present disclosure. In some embodiments based on FIG8, at least one second microfluidic valve 263 is also provided on the third microfluidic channel 261, and the at least one second microfluidic valve 263 can enhance the control force of the third microfluidic channel 261 on the movement of waste liquid from the detection tank 25 to the waste liquid tank 262.

[0058] In some embodiments, referring back to FIG8, in other embodiments based on FIG8, a vent 264 is provided on the waste liquid tank 262. The vent 264 is configured to allow gas to be discharged from the waste liquid tank 262, which helps to collect the waste liquid.

[0059] Referring again to Figure 8, in some other embodiments based on Figure 8, a stepped groove 265 may be provided in the waste liquid tank 262. The depth difference of the stepped groove 265 can help maintain the liquid-gas interface line and prevent the waste liquid in the waste liquid tank 262 from flowing back.

[0060] Please refer to Figures 9 through 11 simultaneously. Figures 9 through 11 are schematic diagrams of the detection chip structure in different embodiments. Figure 9 is a schematic diagram of another detection chip structure according to some embodiments, Figure 10 is a schematic diagram of yet another detection chip structure according to some embodiments, and Figure 11 is a schematic diagram of yet another detection chip structure according to some embodiments.

[0061] As shown in Figure 9, the circular detection chip 1 in the embodiment of Figure 9 includes a sample loading tank 21, a separation unit 22, a diversion unit 23, two injection tanks 24, a detection tank 25, and a waste liquid unit 26.

[0062] The difference between the detection chip 1 shown in the embodiment of Figure 10 and the embodiment of Figure 9 is that the sample loading slot 21 simultaneously corresponds to four detection slots 25. That is, the embodiment of Figure 10 only requires one sample loading to achieve simultaneous detection of four different requirements of the sample. Of course, depending on the detection requirements and quantity, the sample loading slot 21 can be designed to correspond to multiple detection slots 25, and this disclosure does not limit this. The difference between the embodiment of the detection chip 1 shown in Figure 11 and the embodiment of Figure 10 is that the detection chip 1 is divided into fan-shaped disks, and a single detection chip 1 can correspond to a single detection object, which can reduce the waste of the detection chip 1.

[0063] Figure 12 is a schematic diagram of the structure of a detection device according to some embodiments of the present disclosure. Referring to Figure 12, the detection device D of this embodiment includes a drive mechanism 3 and a detection chip 1 mentioned in the foregoing embodiments. The detection chip 1 is detachably mounted on the drive mechanism 3. The drive mechanism 3 provides rotational power, driving the detection chip 1 to rotate, thereby realizing the detection of the sample to be tested within the detection chip 1.

[0064] In some embodiments, please refer to FIG13, which is a schematic diagram of a detection device according to some embodiments of the present disclosure. The detection device D in the embodiment of FIG13 further includes a magnetic mechanism 4 that applies a magnetic force to the detection slot 25 in the detection chip 1.

[0065] By utilizing the magnetic force of the magnetic mechanism 4, the intermediates and reactants can be retained within the detection tank 25, preventing them from being ejected during centrifugation. For example, in this embodiment, the magnetic mechanism 4 has multiple magnetic blocks 41 arranged circumferentially along the detection chip 1. These magnetic blocks 41 can move in directions away from or towards the detection tank 25, thereby controlling the position of the intermediates and reactants within the detection tank 25. Of course, controlling the position of the magnetic blocks 41 can also assist in processes such as mixing or cleaning during the reaction of the intermediates and reactants within the detection tank 25.

[0066] Please refer to Figures 9, 14, and 15A-15G simultaneously. Figure 14 is a flowchart of a detection method according to some embodiments of the present disclosure; Figures 15A-15G are schematic diagrams of a detection chip operation method according to some embodiments of the present disclosure.

[0067] This embodiment will describe in detail the implementation of the detection method embodiment based on the structure of the detection chip 1 shown in the embodiment of FIG9.

[0068] First, step (A) involves adding a sample to be tested into a sample loading chamber. For example, in step (A), the sample to be tested is first added into the sample loading chamber 21 of the detection chip 1 (as shown in Figure 15A). Then, step (B) is performed, which drives the detection chip to rotate and separates the sample from the sample loading chamber into a test sample through a separation unit.

[0069] In step (B), this embodiment drives the detection chip to centrifuge at a first rotational speed, causing the sample to be tested to enter the separation tank 222 through the first flow channel 221, and the sample to be tested is separated into the detection sample and samples of other densities in the separation tank 222 (as shown in Figure 15B). Then, step (C) is performed, and the detection sample is transported to the corresponding at least one detection tank through the splitting unit.

[0070] In step (C), the detection chip is centrifuged at a second rotation speed while changing its rotation direction, so that the liquid level of the detection sample in the first microchannel 223 breaks through the first turning end A3 and enters the diversion channel 231. Under the action of centrifugal force, the detection sample in the diversion channel 231 is first distributed to the quantitative tank 232, and then the excess detection sample is collected into the storage tank 234 (as shown in Figure 15C).

[0071] Next, step (D) is performed to add at least one reaction solution to the at least one detection tank through at least one injection tank, so that the detection sample and the at least one reaction solution are mixed and incubated to obtain a mixture containing at least one intermediate, and then centrifugation is performed to remove a waste liquid from the mixture.

[0072] In step (D), the sample in the quantitative tank 232 is centrifuged at a third rotation speed to break through the first microfluidic valve 235 and enter the detection tank 25 (as shown in Figure 15D). Then, step (E) is performed, in which a cleaning solution is added to the at least one detection tank through the at least one injection tank to clean the at least one intermediate.

[0073] In step (E), the reaction solution is added to the injection tank 24 and centrifuged at the fourth rotation speed to allow the reaction solution to enter the detection tank 25. Then, by alternating forward and reverse rotation, the detection tank 25 is heated to achieve mixing and incubation of the test sample and the reaction solution, resulting in a mixture containing the intermediate (as shown in Figure 15E).

[0074] Finally, step (F) involves adding at least one substrate to at least one detection tank through at least one injection tank to initiate a reaction, and then detecting the reaction result in at least one detection tank. In this step, in this embodiment, the waste liquid in the detection tank 25 is first centrifuged at a fifth rotation speed to allow it to pass through the second microfluidic valve 263 and enter the waste liquid tank 262, and an intermediate containing impurities is obtained in the detection tank 25. Simultaneously, a cleaning solution is added to the injection tank 24, and then centrifuged at a fourth rotation speed to allow the cleaning solution to enter the detection tank 25.

[0075] Based on the above process, the intermediate is thoroughly cleaned by the cleaning solution through forward and reverse rotation. Then, it is centrifuged at a fifth rotation speed, causing the cleaning solution containing impurities to pass through the second microfluidic valve 263 and enter the waste liquid tank 262, where a high-purity intermediate is obtained in the detection tank 25 (as shown in Figure 15F). Next, in step (F), the substrate is added to the injection tank 24, while the detection chip is centrifuged at a fourth rotation speed, allowing the substrate to enter the detection tank 25. Then, the detection chip undergoes forward and reverse rotation while simultaneously heating the detection tank 25 to achieve uniform mixing and reaction of the intermediate and substrate (as shown in Figure 15G). After the reaction is complete, it can be detected and analyzed by the detection device D.

[0076] Among the sample selection methods described above, immunoassay is particularly suitable, especially for detecting antigens in blood. In the examples above, the sample can be plasma; the reaction solution can be a capture carrier containing antibodies bound to its surface and an enzyme-labeled antibody. The capture carrier can be magnetic beads, and the enzyme used for the enzyme-labeled antibody can be alkaline phosphatase (ALP). The substrate can be any one of AMPPD, CDP-Star, or APS-5.

[0077] When using magnetic beads as a capture carrier to detect antigens in blood, an external magnetic field can be used to screen intermediates and retain them in the detection tank 25. By adjusting the distribution of the magnetic field, intermediates can be cleaned to different degrees.

[0078] In other embodiments, when the embodiment shown in FIG10 or 11 is used, that is, when a single sample loading tank 21 corresponds to multiple detection tanks 25 and the sample loading tank 21 is pre-filled with particulate reagents, the difference from the above detection process is that the reaction liquid in the above detection process is replaced with an activation liquid. After the activation liquid is added to the sample loading tank 21 to dissolve and activate the particulate reagents, the dissolved particulate reagents are then transported to the detection tanks 25 by centrifugation.

[0079] Since the reaction solution is a liquid, it has a short shelf life after opening and is more likely to be contaminated. Therefore, pre-setting granular reagents can reduce the waste of the reaction solution and greatly reduce the probability of contamination, thus ensuring the accuracy of the detection.

[0080] Of course, the detection chip, detection device and detection method described above can also be used to perform fully automated detection of multiple indicators in bodily fluids such as urine, saliva, semen, spinal cord or amniotic fluid of human or animal. In addition, this disclosure can also be used in the field of food safety to detect toxic and harmful substances, bacteria or viruses in food. Similarly, this disclosure can be used in the fields of pharmaceuticals and chemicals to detect various drug ingredients and chemical products.

[0081] The specific embodiments described above are merely preferred embodiments of this disclosure and should not be construed as limiting the scope of this disclosure. Simple equivalent changes and modifications made based on the scope of the claims and descriptions of this disclosure are still within the scope of this disclosure. The above are merely exemplary embodiments of this disclosure and do not limit the patent scope of this disclosure. All equivalent structural transformations made using the content of this specification and drawings under the disclosed concept, or direct / indirect applications in other related technical fields, are included within the patent protection scope of this disclosure.

Claims

1. A detection chip, comprising: One sample addition tank; A separation unit is connected to the sample dispensing tank; A shunt unit is connected to the separation unit; At least one detection slot is connected to the diversion unit; At least one injection tank is connected to at least one flow channel that connects the diversion unit and the at least one detection tank; At least one waste liquid unit is connected to the at least one detection tank; Each of the at least one waste liquid unit further includes: A third microchannel, connected to the corresponding detection slot in the at least one detection slot; and A waste liquid tank is connected to the third microchannel.

2. The detection chip according to claim 1, wherein, Each of the at least one waste liquid unit includes a stepped tank.

3. The detection chip according to claim 2, wherein, Each of the at least one waste liquid unit also includes a second microfluidic valve disposed on the third microfluidic channel.

4. A detection device, comprising: One driving mechanism; as well as The detection chip according to any one of claims 1-3 is detachably connected to the drive mechanism.

5. The detection device according to claim 4, wherein, The detection device also includes a magnetic mechanism that applies a magnetic field to at least a portion of the at least one detection slot within the detection chip.

6. A method for detecting a chip, comprising: (A) Add a sample to be tested into a sample loading tank; (B) Drive the detection chip to rotate and separate the sample to be tested from the sample dispensing cell into a detection sample through a separation unit; (C) The test sample is conveyed to at least one corresponding test tank via a diversion unit; (D) At least one reaction solution is added to the at least one detection tank through at least one injection tank, the test sample and the at least one reaction solution are mixed and incubated to obtain a mixture containing at least one intermediate, and then a waste liquid in the mixture is removed by centrifugation. (E) A cleaning solution is added to the at least one detection tank through the at least one injection tank, so that the cleaning solution cleans the at least one intermediate; and (F) At least one substrate is added to the at least one detection cell through the at least one injection cell to carry out a reaction, and the reaction result presented in the at least one detection cell is detected.

7. The detection method for the detection chip according to claim 6, wherein, The sample to be tested is blood.

8. The detection method for the detection chip according to claim 7, wherein, In step (D), the at least one reaction solution contains a capture carrier, which is coated with an antibody.

9. The detection method for the detection chip according to claim 8, wherein, The capture medium is a magnetic bead.

10. The detection method for the detection chip according to claim 8 or 9, wherein, The enzyme used in this antibody is alkaline phosphatase.

11. The detection method for the detection chip according to any one of claims 6-10, wherein, The substrate can be AMPPD, CDP-Star, or APS-5.

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