32results about How to "Rapid detection response" patented technology

The invention relates to a method for synchronously performing parallel detection of various biomarkers and a piece of chip test paper. The method comprises the following steps that: a corresponding ligand An is fixed on a fibrous membrane or other fixed solid holders; the various biomarkers n and a ligand Bn form an affinity binding; and simultaneously the biomarkers move on the holders to combine with at least one ligand with a maker so as to form the ligand An, the biomarkers n, the ligand Bn and a signal marker. A conversion step and a calculation step of release signals comprise the following steps that: through the synchronous excitation of various markers on a piece of detected test paper, the markers generate energy jump after the excitation by exciting light and simultaneously release variant light waves or electronic substances; a detecting instrument receives signals of the light waves or electronic transmitters released by the markers and converts the signals into digital signals; and a data processing system calculates the signal data released by the markers and finally outputs the concentration of the biomarkers. The method can synchronously, quantitatively and quickly detect various biomarkers in one sample, shorten the detection time, and improve the sensitivity and the accuracy.

The invention provides a type of fluorescent probes for detecting formaldehyde. The probes have the structures shown as formulas I to IV. Benzene ring correspondingly substituted coumarin is firstly subjected to backflow reaction with Lawson reagents in toluol; an obtained product continuously performs backflow reaction with hydrazine hydrate in ethanol. The fluorescent probes per se provided by the invention do not have fluorescence in the water solution, but can take a specific fast reaction with formaldehyde to produce products with strong fluorescence, so that the specific sensitive detection and quantitative analysis of formaldehyde is realized. The stability is high; the long-time storage use can be realized; the detection signal-to-noise ratio is high; the sensitivity is high; the excellent selectivity is realized; the specific formaldehyde detection in complicated biological samples can be realized; good biomembrane permeability is realized, so that the fluorescent probes can be used for formaldehyde detection in living cells; the detection reaction is fast; the detection result can be given within 1 minutes for formaldehyde in the water solution; during the formaldehyde detection, the formaldehyde concentration in the environment cannot be influenced. The general molecular formulas of the probes provided by the invention are shown as the accompanying drawing.

The invention discloses a method, system and chip test paper for parallel detection on various cardiac markers. The chip test paper is used for detecting a part or all of the cardiac markers including cTNI (cardiac troponin I), cTNT (cardiac troponin T), MYO (myoglobin), CK-MB (isoenzymeof creatine kinase containing M and B subunits), BNP (B-type natriuretic peptide), CRP (C-reactive protein) and FABP (fatty acid-binding protein); the chip test paper comprises a first membrane and a second membrane; a ligand An of each marker is arranged on the first membrane, and a ligand Bn coupled with a signal marker is absorbed on the second membrane; and a detected material forming sandwich detection together with the ligands An and Bn is added from a sampling hole, then under the acting force of percolation or other factors, the detected material moves to be combined with the ligands An and Bn respectively to form a composite array of the first membrane-the ligand An-the detected material-the ligand Bn-the signal marker, which is fixed on the first membrane, the composite array and a capture fiber membrane are assembled simultaneously, and a detection hole is reserved. The method, the system and the chip test paper provided by the invention can be used for detecting the various cardiac markers simultaneously and quantitatively; moreover, the detection sensitivity can be improved, and the detection time is saved.

The invention provides a photosensitive composite water entry detection device comprises an infrared emitting diode, a photosensitive transistor, a base, an optical probe, an infrared unidirectional light transmitting film, a positive electrode, a negative electrode, a photosensitive detection circuit, a conductive detection circuit and a logic AND circuit, wherein, the photosensitive composite water entry detection device comprises an infrared emitting diode, a photosensitive transistor, a base, an optical probe, an infrared unidirectional light transmitting film, a positive electrode, a negative electrode, a photosensitive detection circuit and a logic AND circuit. A photosensitive composite water inlet detecting device, rapid detection, Simple circuit principle, Reliable function, at present, that practical application of the helicopt floating system is sol, The false triggering phenomenon of water entry detection under the natural environment such as freezing or man-made misoperation conditions, and the non-triggering phenomenon of water entry under the natural strong light environment, therefore, the invention has good reliability and strong pertinence, and can be widely popularized and applied in many fields of multi-type helicopters and offshore life-saving floating platforms, and has great practical value.

The invention relates to a solid-state quantum dot sensor as well as a preparation method and application thereof. Quantum dots are embedded into a polymer matrix formed by perfluorosulfonic acid andpolyvinyl alcohol to form the solid-state quantum dot sensor. Glucose oxidase is embedded into the solid-state quantum dot sensor to generate a solid-state quantum dot glucose sensor. According to thesolid-state quantum dot sensor provided by the invention, the quantum dots and the glucose oxidase are stable in the sensor by specifically selecting a polymer matrix, when the sensor is exposed on atest sample, rapid response can be realized, and the detection efficiency and the sensitivity are improved.

The invention discloses a fluorescent-OCT dual-mode imaging method and device based on indocyanine green nanometer. The method includes: compounding indocyanine green with polyethylene glycol after being treated with phospholipid to form indocyanine green-phospholipid-treated polyethylene glycol nanoparticles, and modifying biologically targeted molecules at the tail ends of polyethylene glycol molecules to obtain an ICG-PL-PEG-FA solution; injecting the ICG-PL-PEG-FA solution to an imaging target, utilizing a low-coherence light source of near infrared to irradiate a to-be-scanned area of theimaging target, and using a light source of 780nm in wavelength to excite ICG-PL-PEG-FA molecules in the imaging target to generate fluorescence; opening an OCT imaging light path to acquire OCT of the imaging target to develop a structure image with a depth range. The fluorescence is generated through the ICG-PL-PEG-FA molecules in the imaging target, an OCT imaging system is applied to collectfluorescent signals, and position information of fluorescent substances in an experimental living body can be reduced, so that lesion area position recognized by a contrast agent can be clearly distinguished, and the structure image of a certain depth range can be presented.

The invention belongs to the technical field of two-photon fluorescence labeling probes and application thereof. The invention particularly relates to a two-photon fluorescence labeling probe, and a synthesis method thereof and application of the two-photon fluorescence labeling probe in novel coronavirus diagnosis. The probe has the chemical name of 3-(N-methyl-N-(6-acetyl-2-naphthyl)amino)propionic acid-N-hydroxysuccinimide ester. The two-photon fluorescent probe has the advantages of high sensitivity and rapid and accurate detection; a fluorescent antigen obtained by labeling an N protein antigen of 2019-nCoV with the probe can be made into an immunochromatographic test strip or a detection kit, and can be used for rapid detection of novel coronavirus, and the test strip or the kit hasthe excellent characteristics of rapid detection, high detection consistency and high stability.

The invention discloses a uric acid test kit capable of eliminating drug interference of calcium dobesilate and etamsylate in serum and a preparation method thereof. According to the technical point,the test kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, peroxidase, ascorbic acid oxidase, a preservative, a Trinder's reactant A, metallate or a nonionic surfactant; and the reagent R2 comprises a buffer solution, serum albumin, uricase, a preservative and a Trinder's reactant B or a nonionic surfactant. According to the uric acid test kit disclosed by the invention, one or more than one of high-redox-potential oxidized metallate, vanadium pentoxide and vanadium oxychloride is / are added into R1 to be mixed, and calcium dobesilate and etamsylate are oxidized by utilizing the metallate, H2O2 generated by uricase in decomposing uric acid after R2 is added is prevented from being consumed, and an accurate uric acid measured value is rapidly obtained.

The invention discloses a toilet device provided with a drying mechanism, belongs to the technical field of toilets, and solves the problem that the use effect is affected due to the fact that the drying mechanism on the conventional toilet device cannot sense the opening and closing states of a baffle plate in time. The toilet device comprises a main body part, a control mechanism, a spray nozzle mechanism, the drying mechanism and a baffle plate sensing mechanism, wherein the main body part is connected with a toilet assembly for supporting a human body; the control mechanism comprises an operating part located on the outer side of the main body part and a control part located in the main body part; the spray nozzle mechanism is mounted at the main body part and used for washing the local private part of the human body; the drying mechanism comprises a casing, an air supply device for producing airflow and a heater for heating the airflow, and the casing is provided with an air outlet for supplying dry air to the human body and a baffle plate for opening or closing the air outlet; the baffle plate sensing mechanism is arranged on the drying mechanism and connected with the control part, and the control part is used for judging the opening and closing states of the baffle plate according to feedback signals of the baffle plate sensing mechanism.

The invention provides a sulfydryl-containing fluorescent compound or a thioester derivative thereof and preparation and application thereof. The sulfydryl-containing fluorescent compound or the thioester derivative of the sulfydryl-containing fluorescent compound can emit strong fluorescence, the compund can directly react with nitric oxide or specifically and rapidly react with nitric oxide after the ester group is removed so as to generate the weak fluorescence product, and the quenched fluorescence can be gradually recovered to the initial level when the nitric oxide concentration is reduced so as to achieve the reversibility and the real-time dynamic detection on nitric oxide. The fluorescent probe is good in stability and can be stored and used for a long time; the detection signal-to-noise ratio is high, and the sensitivity is good; the compound has the advantages of good selectivity, good biofilm permeability, rapid detection reaction and reversibility of detection response andis suitable for real-time dynamic observation of endogenous nitric oxide in organisms such as solutions, intracellular organisms and living tissues. The structural formula of sulfydryl-containing fluorescent compound or the thioester derivative thereof is shown in the specification.

The invention discloses aptamer colloidal gold lateral chromatography test paper for detecting kanamycin, and belongs to the fields of analytical chemistry, medicine, environment, food safety, nano biosensing and the like. According to the invention, the complementary sequences (Aptamer and CapApt) of DNA can be rapidly and sensitively captured by utilizing AuNPs@ polyA-DNA, and the colloidal gold lateral chromatography test strip which is rapid, sensitive and low in cost is developed. The kanamycin detection by the test strip method is simple, convenient and rapid, the detection can be carried out at any time and anywhere, the test result can be observed only by adding a test solution into a sample port and completely developing the test strip after 5 minutes, and the detection efficiency can be greatly improved. Qualitative analysis is carried out by naked eyes, and quantitative analysis is carried out by using a colloidal gold test paper quantitative analyzer. The aptamer colloidal gold lateral chromatography test paper has important significance on kanamycin residue detection in food.

The invention discloses a toilet device provided with a drying mechanism, belongs to the technical field of toilets, and solves the problem that the use effect is affected due to the fact that the drying mechanism on the conventional toilet device cannot sense the opening and closing states of a baffle plate in time. The toilet device comprises a main body part, a control mechanism, a spray nozzle mechanism, the drying mechanism and a baffle plate sensing mechanism, wherein the main body part is connected with a toilet assembly for supporting a human body; the control mechanism comprises an operating part located on the outer side of the main body part and a control part located in the main body part; the spray nozzle mechanism is mounted at the main body part and used for washing the local private part of the human body; the drying mechanism comprises a casing, an air supply device for producing airflow and a heater for heating the airflow, and the casing is provided with an air outlet for supplying dry air to the human body and a baffle plate for opening or closing the air outlet; the baffle plate sensing mechanism is arranged on the drying mechanism and connected with the control part, and the control part is used for judging the opening and closing states of the baffle plate according to feedback signals of the baffle plate sensing mechanism.

The invention discloses an exoskeleton support system of a rotary arm guitar, comprising a first support plate, a second support plate and a hinge. The first support plate and the second support plateare articulated by the hinge, and both the first support plate and the second support plate are provided with openings. A resonance patch, a detection module and an LED light are arranged on the surfaces of the first support plate and the second support plate. The resonance patch is used for resonating with the first support plate and the second support plate and generating an audio signal. The detection module includes a vibration sensor, an analog-to-digital conversion module and a processor. The vibration sensor is used for collecting the audio signal generated by the resonance patch and transmitting the collected audio signal to the analog-to-digital conversion module. The analog-to-digital conversion module converts the received audio signal into a digital signal and sends the digital signal to the processor. The processor processes the received digital signal into a voltage with high and low levels.

The invention relates to an optical coherence tomography method and apparatus based on indocyanine green. The optical coherence tomography method includes the steps: the same amount of phospholipid-polyethylene glycol and the indocyanine green are taken at room temperature and added to distilled water correspondingly to be prepared into solutions, then the two solutions are mixed according to themass ratio of the indocyanine green to the phospholipid-polyethylene glycol being 1:20, and an indocyanine green contrast agent is obtained; the indocyanine green contrast agent is injected into an imaging target, and the indocyanine green contrast agent permeates freely within the imaging target; and an OCT imaging system is opened to obtain images with the depth range produced by OCT imaging ofthe imaging target. According to the optical coherence tomography method and apparatus based on the indocyanine green, the indocyanine green contrast agent based on nanomaterials is used, the effect of optical coherence tomography on image reconstruction of the imaging target is very obvious, the high longitudinal resolution is achieved, the imaging ability is very strong, and the detection response is rapid.

The invention discloses a spectrophotometer with a mixer. The spectrophotometer comprises a spectrophotometer body, wherein a sample feeding tube with a constant-flow pump is arranged in the body, and the sample feeding tube is connected with a cuvette; a fixed seat is arranged in the spectrophotometer body; a sample seat in movable fit connection through a slide bar is arranged on the fixed seat; a clamping port capable of arranging the cuvette is formed in the sample seat, and an optical path hole is formed in the corresponding detection optical path; a vibration motor is connected to the sample seat; a material layout tube is further arranged on the cuvette. According to the device, one or more sample feeding tubes and the corresponding constant-flow pumps can be used for sucking different needed test liquids according to test needs, and laid out in the cuvette, and the vibration motor is capable of rapidly reacting reaction solution in the cuvette and immediately detecting. Therefore, the device disclosed by the invention has the advantages of being suitable for automatic sample feeding and short-time chemical reaction detection tests, and capable of adequately mixing a sample without the need of manual intervention during a mixing process.

The present invention relates to a class of coumarin hydrazone compound selected from the following compounds, its preparation method and application. The coumarin hydrazone compound or its salt itself of the present invention has only weak fluorescence, but it can specifically and quickly react with formaldehyde to generate a product with strong fluorescence, and there is a linear correlation between the fluorescence intensity and the concentration of formaldehyde, thereby realizing the specificity to formaldehyde Sex detection and quantitative analysis. The coumarin hydrazone compound or its salt of the present invention is suitable for the determination of formaldehyde content in the environment, the labeling and imaging of formaldehyde in cells and tissues, the determination of formaldehyde content in biological samples such as tissue homogenate, plasma, urine, saliva, etc., and food Determination of formaldehyde content in life samples such as cosmetics, clothing and so on.

The invention discloses a multi-hole blood detection needle tube. The needle tube comprises a needle tube main body, a needle changing handle is arranged at the top end of the needle tube main body, the needle tube main body is movably connected with the needle changing handle, a test paper box is arranged at the top of the needle tube main body, a long needle tube joint is arranged at the bottomend of the test paper box, a long needle tube is arranged at the bottom end of the long needle tube joint, the long needle tube is fixedly connected with the test paper box through the long needle tube joint, a groove is arranged at the bottom end of the long needle tube, a long needle tube head is arranged at the bottom end of the groove, a needle tube head sleeve is arranged at the bottom end ofthe long needle tube head, the needle tube head sleeve is movably connected with the long needle tube, a blood test needle is arranged in the long needle tube, and a pointed blood test needle head isarranged at the bottom end of the blood test needle. The multi-hole blood detection needle tube has the advantages of convenience in use, fast detection reaction, slight detection pains, and broad market and application prospects.

The invention discloses a submarine cable wear hidden danger detection device and method, and the device is characterized in that the device comprises an excitation source, an armor layer, an output cable, a coated optical fiber, a connection optical fiber, and POTDR equipment; the excitation source is connected with an armor layer of the submarine cable through an output cable and is used for injecting current into the armor layer through the excitation source, and the POTDR equipment is connected with a cladding optical fiber of the submarine cable through a connecting optical fiber and is used for monitoring the polarization state change of the optical fiber and positioning the accurate position of a damaged point according to the sudden change position of the polarization state change of the optical fiber. According to the invention, early warning can be carried out on defect hidden dangers before an accident occurs, and operation and maintenance personnel are reminded to handle the hidden dangers in time.

The invention discloses a light refraction type water detection device. The water detection device comprises a shell, wherein a light source is arranged on one side in the shell, and a first transparent waterproof plate is mounted on one side located in front of the light source in the shell; two photosensitive devices are arranged on the other side in the shell at intervals, the photosensitive device farer from the light source is a first photosensitive device, the photosensitive device nearer to light source is a second photosensitive device, and a second transparent waterproof plate is arranged on the other side in front of the first photosensitive device and the second photosensitive device in the shell; and the included angle between a light-sensitive surface of the first photosensitive device and the second transparent waterproof plate is same as the included angle between an incident ray of the light source and a normal line of the first transparent waterproof plate. The light refraction type water detection device can rapidly respond and be conveniently realized, is relatively low in cost, high in reliability and detection property, is not influenced by the salinity difference of a water body, the atmospheric moisture and height and the like and has a state detection function, so that the online detection of system equipment can be realized.

The invention discloses a steam generation device and aims to provide a steam generation device provided with a water shortage detection device. The water shortage detection device judges precisely, has low cost, quick response and a long service life, and can remind a user to clean heating tubes so as to avoid heating without water. The embodiment of the invention provides the steam generation device which comprises a water distribution valve (1) and the heating tubes (2) which are transparent or partially transparent at the lower part, wherein the lower end of a lower electrode (21) of eachheating tube (2) is provided with a light-emitting device (111) and a light sensitive element (121); and light emitted by the light-emitting device (111) is refracted by at least one of the heating tubes (2) and then reaches the light sensitive element (121) without passing through an axle center of the heating tube (2). The invention also discloses a household electric steam box which comprises the steam generation device.

The invention belongs to the technical field of two-photon fluorescently labeled probes and applications thereof, and in particular relates to two-photon fluorescently labeled probes and a method for synthesizing them, as well as applications in the diagnosis of novel coronaviruses. The chemical name of the probes of the present invention is 3-(N-methyl-N -(6-acetyl-2-naphthyl)amino)propionic acid-N-hydroxysuccinimide ester, the two-photon fluorescent probe has the advantages of high sensitivity, rapid detection, and accuracy, and is used to label the 2019-nCoV The fluorescent antigen obtained after the N protein antigen can be made into immunochromatographic test strips or detection kits, which can be used for the rapid detection of novel coronavirus pneumonia, and the test strips or kits have the advantages of rapid detection, detection consistency and stability High quality features.

A uric acid test kit capable of eliminating the interference of calcium dobesilate and phenetamine drugs in serum and a preparation method thereof of the present invention, the key points of its technical scheme are: comprising reagent R1 and reagent R2, and the reagent R1 contains buffer solution, peroxidase, ascorbate oxidase, preservative, Trinder's reaction reagent A, metal salt or nonionic surfactant; the reagent R2 includes buffer, serum albumin, uricase, preservative, and Trinder's reaction Agent B or nonionic surfactant. In the uric acid kit of the present invention, one or more of the oxidized metal salts of high redox potential or vanadium pentoxide or vanadium oxychloride are added to R1, and the hydroxybenzene Oxidation of calcium sulfonate and phenetamine prevents the consumption of H2O2 produced by uricase decomposing uric acid after adding R2, and quickly obtains accurate uric acid measurement values.

The invention discloses a steam generation device and aims to provide a steam generation device provided with a water shortage detection device. The water shortage detection device judges precisely, has low cost, quick response and a long service life, and can remind a user to clean heating tubes so as to avoid heating without water. The embodiment of the invention provides the steam generation device which comprises a water distribution valve (1) and the heating tubes (2) which are transparent or partially transparent at the lower part, wherein the lower end of a lower electrode (21) of each heating tube (2) is provided with a light-emitting device (111) and a light sensitive element (121); and light emitted by the light-emitting device (111) is refracted by at least one of the heating tubes (2) and then reaches the light sensitive element (121) without passing through an axle center of the heating tube (2). The invention also discloses a household electric steam box which comprises the steam generation device.

The invention discloses a light refraction type water detection device. The water detection device comprises a shell, wherein a light source is arranged on one side in the shell, and a first transparent waterproof plate is mounted on one side located in front of the light source in the shell; two photosensitive devices are arranged on the other side in the shell at intervals, the photosensitive device farer from the light source is a first photosensitive device, the photosensitive device nearer to light source is a second photosensitive device, and a second transparent waterproof plate is arranged on the other side in front of the first photosensitive device and the second photosensitive device in the shell; and the included angle between a light-sensitive surface of the first photosensitive device and the second transparent waterproof plate is same as the included angle between an incident ray of the light source and a normal line of the first transparent waterproof plate. The light refraction type water detection device can rapidly respond and be conveniently realized, is relatively low in cost, high in reliability and detection property, is not influenced by the salinity difference of a water body, the atmospheric moisture and height and the like and has a state detection function, so that the online detection of system equipment can be realized.

A fluorescence-OCT dual-mode imaging method and device based on indocyanine green nanometers, the method comprising: combining indocyanine green with phospholipidated polyethylene glycol to form indocyanine green-phospholipidated polyethylene glycol Nanoparticles, and modify biological targeting molecules at the end of the polyethylene glycol molecule to obtain ICG-PL-PEG-FA solution; inject the ICG-PL-PEG-FA solution to the imaging target, and use the low coherence of near-infrared light The light source irradiates the area to be scanned of the imaging target and uses a light source with a wavelength of 780nm to excite the ICG-PL-PEG-FA molecules in the imaging target to generate fluorescence; open the OCT imaging optical path to obtain the OCT imaging of the imaging target Structural images with depth ranges. The above method and device generate fluorescence through the ICG-PL-PEG-FA molecules in the imaging target, and use the OCT imaging system to collect the fluorescence signals, which can restore the position information of the fluorescent substance in the experimental living body, so that the contrast agent can be clearly distinguished The position of the identified lesion area presents a structural image of a certain depth range.