Simple and convenient separation method for phytophthora parasitica var nicotianae
A technology of tobacco black shank bacteria and separation method, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of bacteria separation failure, cumbersome operation, and separation needs to be sterilized, and achieve simple equipment and high purity High, the effect of maintaining characteristics
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Embodiment 1
[0025] Select the diseased plants with typical symptoms in the field, no other symptoms, no cracking or artificial dissection of the diseased stems, that is, the diseased plants with the diseased pith discs sealed and wrapped in healthy stems as the target diseased plants, and remove the stem tops when collecting , leaves and roots, only keep the diseased stems, wash the surface to remove floating dust, and dry them. Each diseased stem is wrapped and separated with sterilized toilet paper, and placed in a disposable clean sealed bag. To isolate the pathogen in a clean environment, first cut the diseased stem from the upper part of the stem, grasp the upper end of the diseased stem with both hands, and pull the diseased stem until the disc-shaped diseased pith is exposed. Then use flame-sterilized elbow tweezers to pick up the lesion slices and place them in the oat culture medium plate under the flame; The time for placing the slices was 3 to 5 seconds; each sampling point was...
Embodiment 2
[0027] Replace the oat medium plate with the lima bean medium plate, and the others are the same as in Example 1.
Embodiment 3
[0029] Select diseased stems in the manner of Example 1, separate diseased marrow slices and carry out room temperature cultivation, and select target pathogenic bacteria according to the pure white colony form. The pathogenicity of the isolated bacteria was verified by inoculating the susceptible variety Honghua Dajinyuan with the Jungu method, and the strains with a disease severity greater than 1 were pathogenic.
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