A kind of preparation method of gpx7 recombinant protein

A recombinant protein and prokaryotic expression technology, applied in the field of GPx7 recombinant protein preparation, can solve problems such as protein inactivation, and achieve the effects of high degree of purification, good protein activity and simple steps
CN104004722BInactive Publication Date: 2017-10-27上海御先医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
上海御先医药科技有限公司
Publication Date
2017-10-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of biomedicine and particularly relates to a method for preparing a recombinant GPx7 (glutathione peroxidase) protein. The invention discloses a method for preparing a GPx7 recombinant protein. The method comprises the steps of constructing a GPx7 recombinant protein prokaryotic expression plasmid and strain; expressing the GPx7 recombinant protein; extracting the GPx7 recombinant protein and purifying the GPx7 recombinant protein. The method provided by the invention has the advantages of simple steps, low cost and high degree of purification; the prepared protein has good activity. The method is far superior to a metal affinity chromatography used in the prior art.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a method for preparing GPx7 recombinant protein. Background technique

[0002] Glutathione peroxidase (Glutathione peroxidase, GPx) is an important peroxide-decomposing enzyme that widely exists in the body. The GPx that has been discovered so far has included 8 different subtypes, from the GPx1 subtype to the currently discovered GPx8 subtype, which can be subdivided into two main types, one is the subtype containing Sec rare amino acids, and the human source contains selenium There are 5 types of GPx (SecGPx), including GPx1, GPx2, GPx3, GPx4, and GPx6; one is a subtype that does not contain the rare amino acids of Sec, and GPx7 / 8 completely lacks the binding site for GSH.

[0003] In 2004, it was reported for the first time that GPx7 has the function of alleviating polyunsaturated fatty acid-mediated apoptosis. Since GPx7 and GPx4 (PHGPx) have a high similarity in structure and are...

Claims

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