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Application of Naloxone in the Preparation of Drugs for Improving Spatial Learning and Memory Ability

A technology of learning and memory ability and naloxone, which is applied in the direction of drug combination, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of unstable promotion and insignificant promotion, so as to improve efficiency and spatial learning and memory ability Effect

Active Publication Date: 2016-08-24
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: to overcome the shortcomings of insignificant promotion and unstable promotion in the methods for improving learning and memory ability in the prior art. Applications

Method used

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  • Application of Naloxone in the Preparation of Drugs for Improving Spatial Learning and Memory Ability
  • Application of Naloxone in the Preparation of Drugs for Improving Spatial Learning and Memory Ability
  • Application of Naloxone in the Preparation of Drugs for Improving Spatial Learning and Memory Ability

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Experimental program
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Effect test

Embodiment 1

[0031] 1. Obtain RNA from mouse tissue and obtain cDNA

[0032] After the mouse tissue was ground into powder in liquid nitrogen, 1 ml TRIzol (Invitrogen Company) was added to every 100 mg tissue for grinding, and the total volume of the sample should not exceed 10% of the volume of the TRIzol used. Keep the grinding solution at room temperature for 5 minutes, then add 0.2ml of chloroform per 1ml of TRIzol solution, cover the centrifuge tube tightly, and shake the centrifuge tube vigorously by hand for 15 seconds. Take the upper aqueous phase into a new centrifuge tube, add isopropanol at the ratio of 0.5ml isopropanol per 1ml TRIzol solution, place at room temperature for 10 minutes, and centrifuge at 12000g for 10 minutes. Discard the supernatant, add at least 1ml of 75% ethanol for every 1ml of TRIzol solution, vortex and mix, and centrifuge at 7500g for 5 minutes at 4°C. Carefully discard the supernatant, then dry at room temperature or under vacuum for 5-10 minutes, be c...

Embodiment 2

[0064] A combination that improves spatial learning and memory; includes lentivirus encoding NeuroD1 cDNA and naloxone (purchased from Sigma) used independently.

[0065] Application test 1: the effect of naloxone on the analgesic effect of morphine

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Abstract

The present invention relates to the application of naloxone in the preparation of drugs for improving spatial learning and memory, and the invention also provides the combination of naloxone and lentivirus encoding NeuroD1 cDNA in the preparation of drugs for improving spatial learning and memory application. Since there are no obvious side effects, the dose of naloxone can ensure that it has sufficient promoting effect on the ability of spatial learning and memory. The combined use of naloxone of the present invention and the lentivirus encoding NeuroD1 cDNA can effectively improve the adult neurogenesis in the lower layer of the dentate gyrus of the hippocampus due to blocking the influence of endogenous morphine analogues in the brain on the spatial learning and memory ability. Efficiency and spatial learning and memory ability.

Description

technical field [0001] The invention relates to a new application of naloxone, in particular to an application of naloxone in preparing medicines for improving spatial learning and memory ability. Background technique [0002] The original theory was that new nerve cells do not grow in adult animals. However, with the advancement and deepening of scientific research, researchers have discovered that neural stem cells exist in adult animals. Neural stem cells can differentiate into different types of nerve cells under appropriate conditions, thereby supplementing lost nerve cells in the course of life or providing support for a variety of advanced neural activities. The process of differentiation of neural stem cells into different types of nerve cells in adult animals is generally called adult neurogenesis. Although many studies have shown that neural stem cells and adult neurogenesis can widely exist in multiple regions of the brain, it is generally believed that neural s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/485A61K48/00A61P25/28
Inventor 郑辉李文何松蔚孙昊
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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