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Extraction method of triptolide A

A technology of triptolide A and an extraction method, which is applied in the field of traditional Chinese medicine research and can solve problems such as failure to be widely popularized, high toxicity and the like

Inactive Publication Date: 2017-10-17
FUJIAN ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tripterygium wilfordii has the effects of promoting blood circulation and removing blood stasis, clearing away heat and detoxification, reducing swelling and dissipating stagnation, expelling wind and dampness, killing insects and stopping bleeding. It is highly toxic, and its clinical effective dose is close to the poisoning dose, which makes the application of Tripterygium wilfordii not widely popularized so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Crush 10kg of Tripterygium wilfordii plants containing roots, stems and leaves, pass through a 20-mesh sieve, add 5 L / kg of ethanol to soak for 24 hours, and then slowly add 28 L / kg of ethanol for percolation. Collect the dialysis solution once every 500mL, concentrate it to dryness, add 1mL of chloroform to dissolve it and use TLC to perform qualitative detection until the test is negative, stop the percolation, then combine and concentrate the percolation solution to 1 / 10 of the original volume. Ethanol concentrate;

[0025] 2) After adding water to the obtained ethanol concentrate at a volume ratio of 1:1, add ethyl acetate at a volume ratio of 1:1.5 for extraction. Each extraction needs to be shaken 20 times. After each extraction, the collected ethyl acetate The ester solution was concentrated, and qualitatively tested by TLC. After 4 extractions, the extract was negative. The extraction was stopped. Then the ethyl acetate solution obtained from the extraction was ...

Embodiment 2

[0030] 1) Crush 10kg of Tripterygium wilfordii plant containing roots, stems and leaves, pass a 20-mesh sieve, add 5 L / kg of ethanol to soak for 24 hours, and then slowly add 29 L / kg of ethanol for percolation. Collect the dialysis solution once every 500mL, concentrate it to dryness, add 1mL of chloroform to dissolve it and use TLC to perform qualitative detection until the test is negative, stop the percolation, then combine and concentrate the percolation solution to 1 / 10 of the original volume. Ethanol concentrate;

[0031] 2) After adding water to the obtained ethanol concentrate at a volume ratio of 1:1, add ethyl acetate at a volume ratio of 1:1.5 for extraction. Each extraction needs to be shaken 20 times. After each extraction, the collected ethyl acetate The ester solution was concentrated and qualitatively tested by TLC. After 5 extractions, the extract was negative. The extraction was stopped. Then the ethyl acetate solution obtained from the extraction was combined a...

Embodiment 3

[0036] 1) Crush 10kg of Tripterygium wilfordii plant containing roots, stems and leaves, pass a 20-mesh sieve, add 5 L / kg of ethanol to soak for 24 hours, and then slowly add 30 L / kg of ethanol for percolation. Collect the dialysis solution once every 500mL, concentrate it to dryness, add 1mL of chloroform to dissolve it and use TLC to perform qualitative detection until the test is negative, stop the percolation, then combine and concentrate the percolation solution to 1 / 10 of the original volume. Ethanol concentrate;

[0037] 2) After adding water to the obtained ethanol concentrate at a volume ratio of 1:1, add ethyl acetate at a volume ratio of 1:1.5 for extraction. Each extraction needs to be shaken 20 times. After each extraction, the collected ethyl acetate The ester solution was concentrated, and qualitatively tested by TLC. After 6 extractions, the extract was negative. The extraction was stopped. Then the ethyl acetate solution obtained from the extraction was combined ...

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PUM

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Abstract

The invention discloses a common threewingnut root lactone armour extraction method. According to the method, root hearts, root peels, stems and leaves of a common threewingnut root plant serve as raw materials, the raw materials are subjected to multiple steps of crushing, soaking, percolation, extraction, silica gel column chromatography separation and purification, recrystallization and the like, common threewingnut root lactone armour with the purity as high as 99% or above can be extracted, the yield is 0.01% and 10-20 times higher than that of an extraction process reported by existing documents, and the method is an effective method for obtaining a great amount of common threewingnut root lactone armour and can lay a material foundation and provide a scientific basis for research on illuminating chemical ingredients in common threewingnut roots, pharmacologic actions and the like.

Description

Technical field [0001] The invention belongs to the technical field of traditional Chinese medicine research, and specifically relates to a method for extracting triptolide A. Background technique [0002] Tripterygium ( Tripterygium willfordii Hook.f) is a plant of the genus Tripterygium of the family Euonymus, with whole root or peeled root xylem as medicine. Tripterygium wilfordii has the effects of promoting blood circulation and removing blood stasis, clearing heat and detoxification, reducing swelling and dispelling masses, dispelling wind and dampness, killing insects and stopping bleeding. It is clinically used to treat rheumatoid arthritis, systemic lupus erythematosus, nephritis, compulsive spondylitis, etc., but its It is highly toxic, and its clinically effective dose is similar to the toxic dose, which makes the application of tripterygium wilfordii not widely promoted so far. In order to give full play to the medicinal effects of Tripterygium wilfordii, scholars a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
CPCC07J71/0005
Inventor 林绥阙慧卿钱丽萍
Owner FUJIAN ACAD OF MEDICAL SCI
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