Method for grafting and rapid propagation of special germplasm resources of tea trees

A specific and germplasm technology, applied in grafting, botany equipment and methods, horticulture, etc., can solve the problems of variation and degradation, small reproduction coefficient, and not retaining leaves, etc., and achieve the effect of improving survival rate and accelerating propagation

CN106879369AActive Publication Date: 2017-06-23GUIZHOU TEA RES INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2017-06-23

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Abstract

The invention discloses a method for grafting and rapid propagation of special germplasm resources of tea trees. According to the method, vicennial cloned Fuding big white tea is utilized as stocks, the stocks and cions are twigs before semi-lignification under specific stock-cion combination, grafting work is carried out from the first ten days of May to the middle ten days of June, the twigs of the stocks and cions are cut and grafted by utilizing shavers, grafting cuts are bundled by utilizing black plastic tapes, the whole cion and stock grafting cuts are wrapped with colorless polypropylene plastic bags, an awning is erected, the best time is selected to relieve bundled and wrapping plastic bags and remove the awning, and timely and batched topping is performed. By comprehensively utilizing the steps, the survival rate of the special germplasm resources of the tea trees is improved by 80% or above, and the propagation expanding progress can be accelerated for at least 2-3 years by comparing cuttage and transplantation.
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Description

technical field

[0001] The invention belongs to the field of plant breeding, and more specifically relates to a method for grafting and rapid propagation of tea tree specific germplasm resources. Background technique

[0002] my country is the birthplace of tea trees, and its germplasm resources are very rich. However, with the development of science and technology and breeding methods, conventional germplasm resources are difficult to meet the needs of breeding diversity and directional goals to a certain extent. Therefore, it is necessary to discover and innovate Specific tea germplasm resources.

[0003] Tea tree specific germplasm resources refer to rare germplasm resources with special traits, such as high tea polyphenols, high amino acid, high theanine, low caffeine, albinism and other specific tea tree resources. For the specific tea tree germplasm resources that have been initially excavated, how to speed up the multiplication, speed up resource evaluation, identific...

Examples

Embodiment 1

[0034] A total of 10 special tea germplasm resources including Guilu 1, Guilv 2, Guilv 3, Gelu, Gaoyuanlv, Yiwei, Liufang, Qianjiangyue, Hannan 1 and Baihua 1 were grafted and rapidly propagated, including Follow the steps below:

[0035] (1) Treatment of rootstocks: take the 20-year-old clone Fuding Dabai tea as rootstocks, mow in May to June, and form rootstock stumps with a height of 15 to 25 cm from the ground. Select and stay 10-11 strong new shoots in the direction, and cultivate the new shoots into semi-lignified rootstock twigs with a length of 8-10 cm for grafting.

[0036] (2) The processing of scion: with the twig before the semi-lignification of tea tree specific germplasm resource as scion branch, scion branch is cut into partial wedge-shaped short ear with razor, the long 2~3cm of cutting surface, the long 3~3cm of short ear. 4cm, leave a strong and full axillary bud and a leaf, and retain half of the leaf for the large-leaf variety, and ensure that the cut is o...

experiment example 1

[0048] 10 clumps of tea trees grafted in embodiment 1, 50 branches of each clump, establish 3 repetitions, and graft 150 branches altogether; Observe the survival rate after the summer drought at the beginning of September, and observe the tree height, tree height and tree height when the growth stops at the beginning of November for 3 consecutive years. Width and the number of ears that can be cut.

[0049] Table 1. Grafting survival rate of 10 tea tree specific germplasm resources

[0050]

[0051] The results of the grafted survival rates of 10 tea tree specific germplasm resources are shown in Table 1, and the survival rates are all higher than 80%, indicating that the grafting rapid propagation method of the present invention is suitable for tea tree specific germplasm resources.

[0052] Table 2. The growth vigor and the number of pruned ears of 10 tea tree specific germplasm resources after grafting

[0053]

[0054]

[0055] As shown in Table 2, after the gra...

experiment example 2

[0057] Using the methods of Example 1 (using the grafting and rapid propagation method of the present invention) and Comparative Example 1 (using the conventional cutting and transplanting method) to propagate a total of 3 tea tree specific germplasm resources of No. 1, No. 2 and No. 3 , and observed the tree height, tree width and the number of pruned ears at the beginning of November when the growth stopped for three consecutive years.

[0058] Table 3. Comparison of the growth vigor and the number of pruned ears of the three tea tree specific germplasm resources under different multiplication methods

[0059]

[0060] As shown in Table 3, the tree height of Guilu No. 1 in the first year of grafting was 61.7cm, which was 2.8cm higher than that in the third year of cutting transplantation, and the number of ears that could be cut in the second year of grafting was 19.1 more than that in the third year of cutting transplantation; Green No. 2 is 63.5cm taller in the first ye...