A fully automatic processing platform integrating fatty acid value pretreatment and titration and a processing method thereof

Abstract

The invention discloses a full-automatic processing platform integrating fatty acid value pretreatment and titration and a processing method thereof. The full-automatic processing platform comprises a platform body; the full-automatic processing platform comprises a sample container, a filter, a liquid storage tube, a distilled water container, a KOH solution container and an indicator container located on both sides of the platform body; the full-automatic processing platform comprises a fluid pump and a titration pump located inside the platform body, and a pipetting table and a titration chamber located on the upper end of the platform body; a pipetting cup is arranged on the top of the pipetting table; the instrument pretreatment process and the sample titration process are both fully automated without human intervention in the middle, thus avoiding human operation errors, greatly saving manpower and time, and greatly improving work efficiency, and well solving the difficult problems currently faced by grain and oil departments in fatty acid value detection.

Description

Technical Field

[0001] The invention relates to the field of food fatty acid value processing systems, and in particular to a full-automatic processing platform integrating fatty acid value pre-processing and titration and a processing method thereof. Background Art

[0002] The fatty acid value of grains is a measurement of the free fatty acid content in grains. The test result is expressed as the amount of potassium hydroxide required to neutralize the free fatty acids in 100g of grain samples. The change in fatty acid value reflects the degree of quality deterioration of rice and corn. Therefore, it is used as an indicator of the suitability of rice and corn in the national standard for grain storage judgment rules.

[0003] During the process of maturation, harvesting and storage of grains, their lipid components will undergo hydrolysis to produce free fatty acids due to the influence of endogenous enzymes and external factors such as temperature and humidity. External environmental factors such as insects, rodents, and mold will accelerate the hydrolysis reaction and increase the free fatty acid content. The international GB / T20569-2006 and GB / T20570-2015 respectively stipulate that fatty acid value is one of the indicators for objectively evaluating the storage quality of rice and corn.

[0004] The existing food fatty acid value pretreatment equipment has a complicated operation process and uses a lot of glassware, which results in a large amount of manual workload and working time in the whole process, reducing work efficiency. The determination and processing process of the fatty acid value of all samples is a unified organic solvent extraction, shaking, filtering operation process and the subsequent determination of the titration endpoint. There are the following specific defects:

[0005] The extraction time is too long, which greatly reduces the efficiency of extraction. After the extraction is completed, it is necessary to manually transfer with filter paper and burette, which is time-consuming and cumbersome. In addition, some grassroots personnel are still lacking in the use of burettes, resulting in inaccurate pipetting, which directly affects the final experimental results. As a result, grassroots grain depots and grain and oil monitoring stations at all levels and other grain departments cannot obtain test results efficiently and accurately when conducting large-scale sample testing. In manual titration, due to the turbidity of the fatty acid sample test liquid and the interference of the color of the test liquid itself, it is very difficult to accurately observe the color endpoint. In addition, different people have different understandings of the slightly red endpoint required by the national standard, resulting in the accuracy and stability of the experimental results cannot be guaranteed during manual titration.

[0006] Therefore, it is necessary to provide a platform that integrates the fatty acid value detection process to solve the difficulties currently faced by the grain and oil departments in fatty acid value detection. Summary of the invention

[0007] In view of the shortcomings of the prior art, the present invention provides a fully automatic processing platform and a processing method thereof that integrates fatty acid value pretreatment and titration, eliminates human interference, uses filters and diaphragm pumps to extract sample solutions, and can accurately extract and pipette liquids, thereby greatly improving the efficiency and accuracy of the entire detection results of fatty acid values ​​by grain and oil departments.

[0008] A fully automatic processing platform integrating fatty acid value pretreatment and titration.

[0009] The fully automatic processing platform includes a platform body;

[0010] The fully automatic processing platform comprises a sample container 1, a filter 2, a liquid storage tube 3, a distilled water container 11, a KOH solution container 12 and an indicator container 13 located on both sides of the platform body;

[0011] The fully automatic processing platform includes a fluid pump 7 and a titration pump 8 located inside the platform body, and a pipetting platform 5 and a titration chamber 10 located at the upper end of the platform body;

[0012] A pipetting cup 6 is arranged on the top of the pipetting platform 5;

[0013] The sample container 1, the filter 2 and the liquid storage tube 3 are connected to the fluid pump 7 inside the platform body through a hose, and then connected to the pipetting cup 6 through a hose;

[0014] The distilled water container 11 and the KOH solution container 12 are connected to the fluid pump 7 through hoses and then connected to the titration chamber 10 respectively;

[0015] The indicator container 13 is connected to the titration pump 8 and the titration chamber 10 in sequence through a hose.

[0016] Preferably, an observation door 9 is also provided on the front side of the platform body.

[0017] Preferably, a protective cover 4 is disposed above the platform body, and the protective cover 4 is buckled above the pipetting platform 5 .

[0018] Preferably, the number of the sample containers 1 does not exceed eight.

[0019] Preferably, the indicator in the indicator container 13 is a phenolphthalein indicator.

[0020] Preferably, the capacity of the distilled water container 11 is greater than 50 ml.

[0021] Preferably, the capacity of the pipette cup 6 is greater than 25 ml.

[0022] Preferably, an internal diaphragm small water pump is connected between the filter 2 and the liquid storage tube 3.

[0023] A fully automatic processing method integrating fatty acid value pretreatment and titration, the fully automatic processing method comprising the following steps:

[0024] S1, collect eight samples, place them in eight sample containers, stir or shake, and then pass the liquid through the filter and the internal diaphragm small water pump through the hose to the liquid storage tube;

[0025] S2, through the fluid pump, first lubricate the tube with about 4ml and discharge it into the waste tank, then extract 25ml of liquid into the pipette cup;

[0026] S3, the solution in each pipetting cup is sent into the titration chamber through the pipetting station. After entering the titration chamber, 50 ml of distilled water in the distilled water container is added through the fluid pump, and a small amount of indicator in the indicator container is also added;

[0027] S4, then wait for 5 seconds, start automatic titration, add KOH solution into the KOH solution container through the titration pump, and obtain the exact volume consumed. When the liquid color turns slightly red, stop titration;

[0028] S5, the fatty acid value content in the sample solution is calculated by the software, and the pipetting station continues to send the next sample solution into the pipetting cup for titration. After the titration is completed, the pipetting station returns to position 0 to prepare for the next experiment.

[0029] The beneficial effects of the present invention are:

[0030] The invention provides a fully automatic processing platform and a processing method thereof that integrates fatty acid value pretreatment and titration. In terms of processing, 8 samples can be processed at the same time. High-speed stirring extraction only takes 2-3 minutes. After the extraction is completed, the machine automatically extracts the filtrate quickly and quantitatively and accurately absorbs 25 ml of the filtrate required for the experiment. When the filtrate is added to the designated sample test cup, the instrument automatically adds 50 ml of distilled water and phenolphthalein indicator to the cup. When the instrument is used for titration, because the software sets a unified color difference value, the disadvantages of manual titration can be avoided, and the accuracy and repeatability of the results can be guaranteed.

[0031] The platform's pre-treatment process and sample titration process are fully automated, with no human intervention in the middle, avoiding human operation errors, greatly saving manpower and time, and greatly improving work efficiency. This new instrument is fast in pre-treatment, accurate and stable in results. Compared with the traditional manual method, it has great advantages for grassroots grain depots and grain and oil monitoring stations at all levels to conduct large-scale sample testing in grain departments, and it has solved the current difficulties faced by grain and oil departments in fatty acid value testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0033] Figure 1 It is a structural schematic diagram of a fully automatic processing platform integrating fatty acid value pre-treatment and titration of the present invention;

[0034] Explanation of the accompanying drawings: In the figure, 1-sample container; 2-filter; 3-liquid storage tube; 4-protective cover; 5-pipette platform; 6-pipette cup; 7-fluid pump; 8 titration pump; 9-observation door; 10-titration chamber; 11-distilled water container; 12-KOH solution container; 13-indicator container. DETAILED DESCRIPTION

[0035] The specific implementation modes of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0036] In order to fully understand the embodiments of the present invention, detailed structures will be presented in the following description. Obviously, the implementation of the embodiments of the present invention is not limited to the specific details familiar to those skilled in the art. The preferred embodiments of the present invention are described in detail below, but in addition to these detailed descriptions, the present invention may also have other embodiments.

[0037] This embodiment refers to Figure 1 , Figure 1 The schematic diagram of the structure of the fully automatic processing platform of the present invention which integrates fatty acid value pre-treatment and titration.

[0038] The fully automatic processing platform shown in the figure integrates fatty acid value pretreatment and titration. The fully automatic processing platform includes a platform body; the fully automatic processing platform includes a sample container 1, a filter 2, a liquid storage tube 3, a distilled water container 11, a KOH solution container 12 and an indicator container 13 located on both sides of the platform body, and the number of the sample containers 1 does not exceed 8; the fully automatic processing platform includes a fluid pump 7 and a titration pump 8 located inside the platform body, and a pipetting table 5 and a titration chamber 10 located at the upper end of the platform body; a pipetting cup 6 is arranged on the top of the pipetting table 5; the sample container 1, the filter 2 and the liquid storage tube 3 are connected to the fluid pump 7 inside the platform body through a hose, and then through the hose connected to the pipetting cup 6; the distilled water container 11 and the KOH solution container 12 are connected to the fluid pump 7 through hoses and then connected to the titration chamber 10 respectively; the indicator container 13 is connected to the titration pump 8 and the titration chamber 10 in sequence through a hose; an observation door 9 is also provided on the front side of the platform body for the convenience of maintenance; a protective cover 4 is provided above the platform body, and the protective cover 4 is buckled above the pipetting platform 5 to play a dust-proof role; the indicator in the indicator container 13 is a phenolphthalein indicator, the capacity of the distilled water container 7 is greater than 50ml, the capacity of the pipetting cup 5 is greater than 25ml, and an internal diaphragm small water pump is connected between the filter 2 and the liquid storage tube 3.

[0039] Specifically, the sample container 1 is an absolute ethanol sample solution, and the number is eight. Eight samples are collected and placed in eight sample containers. After stirring or shaking, the liquid is passed through the filter 2 and the internal diaphragm small water pump through the hose to the liquid storage tube 3 (in order to maintain the water level and pressure balance, it is first stored in the liquid storage tube); then it passes through the fluid pump 7, first moistens the tube with about 4 ml, and discharges it into the waste liquid tank, and then extracts 25 ml of liquid into the pipette cup 6, and the solution in each pipette cup 6 is sent to the titration chamber 10 through the pipetting table 5. After entering the titration chamber 10, 50 ml of distilled water is added to the distilled water container 11 through the fluid pump 7, and a small amount of indicator is also added to the indicator container 13. Then wait for 5 seconds, start automatic titration, add KOH solution into KOH solution container 12 through titration pump 8, and get the exact volume consumed. When the liquid color turns slightly red, stop titration, and calculate the fatty acid value content in the sample solution through software. The pipetting station 5 continues to feed the pipetting cup 6 of the next sample solution for titration. After the titration is completed, the pipetting station returns to position 0 to prepare for the next experiment. The observation door 9 is for the convenience of maintenance preparation and observation.

[0040] The present embodiment is a fully automatic processing platform that integrates fatty acid value pretreatment and titration. In terms of pretreatment, it can process 8 samples at a time. High-speed stirring extraction only takes 2-3 minutes, while the traditional method takes 10 minutes or 30 minutes. After the extraction is completed, the machine automatically extracts the filtrate quickly and quantitatively and accurately absorbs the 25 ml of filtrate required for the experiment. When the filtrate is added to the designated sample test cup, the instrument automatically adds 50 ml of distilled water and phenolphthalein indicator to the cup.

[0041] In this embodiment, corn is taken as an example. The entire pretreatment takes about 10 minutes, while the traditional method takes about 60 minutes, and the work efficiency is greatly improved. After the pretreatment is completed, the sample test cup containing the sample liquid to be tested will automatically enter the titration chamber to perform the titration work of the experiment. The software automatically determines the experimental endpoint according to the change in the color of the sample liquid during the titration process; the instrument is used for titration, because the software sets a unified color difference value, the shortcomings of manual titration can be avoided, and the accuracy and repeatability of the results are guaranteed; the instrument pretreatment process and sample titration process are fully automated, without human intervention in the middle, avoiding human operation errors, greatly saving manpower and time, and greatly improving work efficiency; this new instrument is fast in pretreatment, accurate and stable in results, and has great advantages over the traditional manual method when conducting large-scale sample testing for food departments such as grassroots grain depots and grain and oil monitoring stations at all levels, and well solves the current difficulties faced by grain and oil departments in fatty acid value testing.

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field can still modify or replace the specific implementation methods of the present invention with equivalents. Any modifications or equivalent replacements that do not depart from the spirit and scope of the present invention are within the scope of protection of the pending claims.

Claims

1. A fully automatic processing platform integrating fatty acid value pretreatment and titration, characterized in that: The fully automatic processing platform includes a platform body; The fully automatic processing platform comprises a sample container (1), a filter (2), a liquid storage tube (3), a distilled water container (11), a KOH solution container (12) and an indicator container (13) located on both sides of the platform body; The fully automatic processing platform comprises a fluid pump (7) and a titration pump (8) located inside the platform body, and a pipetting platform (5) and a titration chamber (10) located at the upper end of the platform body; A pipetting cup (6) is arranged on the top of the pipetting platform (5); The sample container (1), the filter (2) and the liquid storage tube (3) are connected to the fluid pump (7) inside the platform body through a hose, and then connected to the pipetting cup (6) through a hose; The distilled water container (11) and the KOH solution container (12) are respectively connected to the fluid pump (7) through hoses and then respectively connected to the titration chamber (10); The indicator container (13) is connected to the titration pump (8) and the titration chamber (10) in sequence through a hose.

2. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: An observation door (9) is also provided on the front side of the platform body.

3. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: A protective cover (4) is arranged above the platform body, and the protective cover (4) is buckled above the pipetting platform (5).

4. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: The number of the sample containers (1) does not exceed 8.

5. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: The indicator in the indicator container (13) is a phenolphthalein indicator.

6. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: The capacity of the distilled water container (11) is greater than 50 ml.

7. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: The capacity of the pipetting cup (6) is greater than 25 ml.

8. The fully automatic processing platform integrating fatty acid value pre-treatment and titration according to claim 1, characterized in that: An internal diaphragm small water pump is connected between the filter (2) and the liquid storage pipe (3).

9. A fully automatic processing method integrating fatty acid value pretreatment and titration, the fully automatic processing method is based on the fully automatic processing platform integrating fatty acid value pretreatment and titration according to claims 1-8, characterized in that: The fully automatic processing method comprises the following steps: S1, collect eight samples, place them in eight sample containers, stir or shake, and then pass the liquid through the filter and the internal diaphragm small water pump through the hose to the liquid storage tube; S2, through the fluid pump, first lubricate the tube with 4ml and discharge it into the waste tank, then extract 25ml of liquid into the pipette cup; S3, the solution in each pipetting cup is sent into the titration chamber through the pipetting station. After entering the titration chamber, 50 ml of distilled water in the distilled water container is added through the fluid pump, and a small amount of indicator in the indicator container is also added; S4, then wait for 5 seconds, start automatic titration, add KOH solution into the KOH solution container through the titration pump, and obtain the exact volume consumed. When the liquid color turns slightly red, stop titration; S5, the fatty acid value content in the sample solution is calculated by the software, and the pipetting station continues to send the next sample solution into the pipetting cup for titration. After the titration is completed, the pipetting station returns to position 0 to prepare for the next experiment.

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