Velvet antler extract as well as preparation method and application thereof

Abstract

The invention discloses a preparation method of a velvet antler. The preparation method is characterized by comprising the following steps: 1) selecting and placing velvet antler in a clear water forsoaking treatment for 5-10 h, taking out the velvet antler and steaming the velvet antler with steam for 15-20 min, then, placing the velvet antler in water at 50-65 DEG C for soaking treatment for 1-2 h, and producing the treated velvet antler into freeze-dried powder; 2) adding the freeze-dried powder to an extraction solvent, and carrying out reflux extraction on the velvet antler to obtain a reflux liquid; 3) performing filtration by a vacuum suction filter to remove impurities in the reflux liquid to obtain a primary filtrate for later use; 4) introducing the primary filtrate in a crusherfor cell crushing treatment, releasing active substances in cells through ultrasonic dispersing treatment, dissolving the active substances in the extraction solvent, and performing filtration by a superfine filter to obtain a secondary filtrate; and 5) concentrating and drying the secondary filtrate to obtain the velvet antler extract. The method is beneficial to dissolution of bioactive substances and effective components in the reflux process.

Description

technical field

[0001] The invention belongs to the technical field of deer antler extraction, in particular to antler extract and its preparation method and application.

[0002] technical background

[0003] Antler (Cornucer vipantotrichum) is the unossified and densely hairy young horns of the male deer (Cervus Nippon Temminch) or red deer (Cervuselaphus Linaeus) of the deer family. It is a traditional Chinese medicine with a history of more than 2,000 years. Deer antler is a precious medicinal material in traditional Chinese medicine. Together with ginseng and donkey-hide gelatin, it is called the three treasures of traditional Chinese medicine. It has a history of more than 2,000 years of use.

[0004] Modern pharmacological studies have confirmed that the chemical components in velvet antler are complex, among which the protein content is as high as 55.26%, which has a positive regulatory effect on the cardiovascular system, nervous system and sexual function. However,...

Examples

Embodiment 1

[0035] A preparation method of deer antler extract, comprising the following contents:

[0036] 1) Select velvet antler and soak it in clean water for 5 hours; take it out and steam it for 15 minutes, then soak it at 50°C for 1 hour; make the processed velvet antler into freeze-dried powder;

[0037] 2) Immerse the freeze-dried powder in absolute ethanol, heat it in a water bath, and the temperature of the water bath is 80°C, and carry out reflux extraction on the velvet antler to obtain the reflux liquid; the solid-liquid ratio of the freeze-dried powder and absolute ethanol is 1:20;

[0038] 3) filter with a vacuum filter to remove impurities in the reflux liquid to obtain a primary filtrate, and store the primary filtrate statically for 10 hours for subsequent use;

[0039] 4) Pass the primary filtrate into the crusher to crush the cells, and then undergo ultrasonic dispersion treatment to release the active substances in the cells and dissolve them in the extraction medium...

Embodiment 2

[0042] A preparation method of deer antler extract, comprising the following contents:

[0043] 1) Select velvet antler and soak it in clean water for 7 hours; take it out and steam it for 16 minutes, and then soak it at 55°C for 1.5 hours; make the processed velvet antler into freeze-dried powder;

[0044] 2) Immerse the freeze-dried powder in absolute ethanol, heat it in a water bath, and the temperature of the water bath is 80°C, and carry out reflux extraction on the velvet antler to obtain the reflux liquid; the solid-liquid ratio of the freeze-dried powder and absolute ethanol is 1:20;

[0045] 3) filter with a vacuum filter to remove impurities in the reflux liquid to obtain a primary filtrate, and store the primary filtrate statically for 12 hours for subsequent use;

[0046] 4) Pass the primary filtrate into the crusher to crush the cells, and then undergo ultrasonic dispersion treatment to release the active substances in the cells and dissolve them in the extraction...

Embodiment 3

[0049] A preparation method of deer antler extract, comprising the following contents:

[0050] 1) Select velvet antler and soak it in clean water for 8 hours; take it out and steam it for 17 minutes, and then soak it at 60°C for 2 hours; make the processed velvet antler into freeze-dried powder;

[0051] 2) Immerse the freeze-dried powder in absolute ethanol, heat it in a water bath at a temperature of 85°C, and extract the velvet antler by reflux to obtain a reflux liquid; the solid-liquid ratio of the freeze-dried powder to absolute ethanol is 1:25;

[0052] 3) Filtrate with a vacuum filter to remove impurities in the reflux liquid to obtain a primary filtrate, and store the primary filtrate for 14 hours for subsequent use;

[0053] 4) Pass the primary filtrate into the crusher to crush the cells, and then undergo ultrasonic dispersion treatment to release the active substances in the cells and dissolve them in the extraction medium. The frequency of ultrasonic treatment is...

technical field

[0001] The invention belongs to the technical field of deer antler extraction, in particular to antler extract and its preparation method and application.

[0002] technical background

[0003] Antler (Cornucer vipantotrichum) is the unossified and densely hairy young horns of the male deer (Cervus Nippon Temminch) or red deer (Cervuselaphus Linaeus) of the deer family. It is a traditional Chinese medicine with a history of more than 2,000 years. Deer antler is a precious medicinal material in traditional Chinese medicine. Together with ginseng and donkey-hide gelatin, it is called the three treasures of traditional Chinese medicine. It has a history of more than 2,000 years of use.

[0004] Modern pharmacological studies have confirmed that the chemical components in velvet antler are complex, among which the protein content is as high as 55.26%, which has a positive regulatory effect on the cardiovascular system, nervous system and sexual function. However,...

Examples

Embodiment 1

[0035] A preparation method of deer antler extract, comprising the following contents:

[0036] 1) Select velvet antler and soak it in clean water for 5 hours; take it out and steam it for 15 minutes, then soak it at 50°C for 1 hour; make the processed velvet antler into freeze-dried powder;

[0037] 2) Immerse the freeze-dried powder in absolute ethanol, heat it in a water bath, and the temperature of the water bath is 80°C, and carry out reflux extraction on the velvet antler to obtain the reflux liquid; the solid-liquid ratio of the freeze-dried powder and absolute ethanol is 1:20;

[0038] 3) filter with a vacuum filter to remove impurities in the reflux liquid to obtain a primary filtrate, and store the primary filtrate statically for 10 hours for subsequent use;

[0039] 4) Pass the primary filtrate into the crusher to crush the cells, and then undergo ultrasonic dispersion treatment to release the active substances in the cells and dissolve them in the extraction medium...

Embodiment 2

[0042] A preparation method of deer antler extract, comprising the following contents:

[0043] 1) Select velvet antler and soak it in clean water for 7 hours; take it out and steam it for 16 minutes, and then soak it at 55°C for 1.5 hours; make the processed velvet antler into freeze-dried powder;

[0044] 2) Immerse the freeze-dried powder in absolute ethanol, heat it in a water bath, and the temperature of the water bath is 80°C, and carry out reflux extraction on the velvet antler to obtain the reflux liquid; the solid-liquid ratio of the freeze-dried powder and absolute ethanol is 1:20;

[0045] 3) filter with a vacuum filter to remove impurities in the reflux liquid to obtain a primary filtrate, and store the primary filtrate statically for 12 hours for subsequent use;

[0046] 4) Pass the primary filtrate into the crusher to crush the cells, and then undergo ultrasonic dispersion treatment to release the active substances in the cells and dissolve them in the extraction...

Embodiment 3

[0049] A preparation method of deer antler extract, comprising the following contents:

[0050] 1) Select velvet antler and soak it in clean water for 8 hours; take it out and steam it for 17 minutes, and then soak it at 60°C for 2 hours; make the processed velvet antler into freeze-dried powder;

[0051] 2) Immerse the freeze-dried powder in absolute ethanol, heat it in a water bath at a temperature of 85°C, and extract the velvet antler by reflux to obtain a reflux liquid; the solid-liquid ratio of the freeze-dried powder to absolute ethanol is 1:25;

[0052] 3) Filtrate with a vacuum filter to remove impurities in the reflux liquid to obtain a primary filtrate, and store the primary filtrate for 14 hours for subsequent use;

[0053] 4) Pass the primary filtrate into the crusher to crush the cells, and then undergo ultrasonic dispersion treatment to release the active substances in the cells and dissolve them in the extraction medium. The frequency of ultrasonic treatment is...