A near-infrared femtosecond laser-induced vascular embolization system

Abstract

The present invention discloses a near-infrared femtosecond laser simulation-induced vascular embolism system. Rose Bengal reagent is pre-injected into the cerebral cortical blood vessels; the femtosecond laser is stimulated to generate a specific near-infrared 1070nm femtosecond laser and is incident on the two-photon microscope system to focus on the cerebral cortical blood vessel area of ​​interest; repeated spiral rapid scanning stimulation is performed in the area of ​​interest ROI to form a thrombus, and the imaging laser is also processed to generate an imaging beam and focused on the cerebral cortical blood vessels for real-time vascular imaging; the imaging laser generates an imaging beam and is incident on the two-photon microscope system; the stimulating femtosecond laser and the imaging laser are controlled to work according to the scanning imaging sequence. The present invention combines 1070nm near-infrared femtosecond laser stimulation with two-photon microscopic imaging technology to achieve precise and rapid thrombosis of blood vessels of various types, sizes and depths. It is a new and more effective system for the formation of targeted vascular thrombus, which is fast, accurate and has depth selectivity.

Description

Technical Field

[0001] The present invention relates to a simulation induction system for cerebral cortical vascular embolism, in particular to a simulation induction system for inducing rapid, precise and depth-selective vascular embolism by combining near-infrared femtosecond laser to excite rose bengal. Background Art

[0002] Studying vascular changes in the cerebral cortex is crucial to understanding the mechanisms of blood flow regulation during healthy brain function and its relationship to neurovascular and functional homeostasis in brain disease states.

[0003] Traditionally, in vivo cerebral cortical vascular thrombosis has been developed to study the impact of vascular dysfunction in health and disease states, but key technical deficiencies remain, such as the depth selectivity of target thrombus location as well as accuracy and speed.

[0004] In previous studies, a variety of systems have been proposed to achieve cortical vascular embolization. The use of green light can induce single-vessel embolism on the cortical surface, but it is limited by spatial resolution and penetration depth. During the photothrombosis process, it may cause non-target excitation of defocused laser light, which may cause thrombosis of the upper vessels of the target thrombus.

[0005] In addition, there are studies that use modulated 800nm ​​high-energy pulsed femtosecond lasers to directly act on vascular endothelial cells, producing photothermal damage to induce a coagulation cascade reaction to produce thrombi. However, this may cause photodamage to the blood vessel wall, and it is difficult to embolize deep blood vessels. Its stability and practicality are also still insufficient.

[0006] In general, because the efficiency, accuracy and penetration depth of thrombosis may be affected by the wavelength, power and scanning mode of the laser, current research and existing technologies lack a complete, accurate and reliable thrombosis simulation induction system for single vessels in the deep cortex, especially a controllable embolism simulation induction technology for single vessels in the deep cortex that has both depth and accuracy. Summary of the invention

[0007] In order to solve the problems existing in the background technology, the present invention provides a near-infrared femtosecond laser stimulation-induced vascular embolization system. The first-of-its-kind femtosecond laser with a wavelength of 1070nm is combined with two-photon microscopy technology. While real-time vascular imaging is performed, the 1070nm femtosecond laser is modulated and spirally scanned to activate the intravascular rose bengal reagent to produce reactive oxygen that damages vascular epithelial cells and causes platelet accumulation, quickly forming single-vessel embolism with high precision and depth.

[0008] This technology provides a single-vessel thrombosis simulation-inducing system with real-time observable thrombosis formation and high spatial selectivity, which is expected to provide a more effective basic research method for studying brain function in health and disease states.

[0009] In order to achieve the above-mentioned purpose, the system of the technical solution adopted by the present invention to solve the technical problem includes:

[0010] The method comprises a color developing reagent, wherein the color developing reagent comprises a rose bengal reagent and a fluorescein dextran dye, which are pre-injected into the blood vessels of the cerebral cortex;

[0011] It includes stimulating a femtosecond laser, generating a specific near-infrared 1070nm femtosecond laser and injecting it into a two-photon microscope system;

[0012] It includes an imaging laser, which generates a specific 920nm femtosecond laser to be incident on a two-photon microscope system;

[0013] It includes a two-photon microscope system, which focuses the femtosecond laser emitted by the stimulation femtosecond laser on the region of interest at the target depth of the cerebral cortical blood vessels, performs specific spiral scanning stimulation in the region of interest ROI to form a thrombus; and processes and focuses the femtosecond laser emitted by the imaging laser on the cerebral cortical blood vessels for imaging;

[0014] Focusing the femtosecond laser from the imaging laser on the blood vessels in the cerebral cortex

[0015] The device comprises a controller, which is respectively connected with the stimulation femtosecond laser and the imaging laser to control the stimulation femtosecond laser and the imaging laser to work according to specific parameters and scanning imaging sequence.

[0016] The stimulation femtosecond laser further comprises:

[0017] It includes a femtosecond laser, which generates a 1070nm femtosecond laser and is incident on a galvanometer group;

[0018] It includes a laser power controller for adjusting the power of the femtosecond laser emitted by the femtosecond laser;

[0019] It includes a stimulating mirror group, which controls the emission position of the laser after being modulated by the stimulating mirror group and then incident on the two-photon microscope system;

[0020] The device comprises a modulator connected to a stimulation galvanometer group, wherein the modulator controls the modulation mode of the stimulation galvanometer group, and modulates the near-infrared femtosecond laser into a repeated spiral scanning mode starting from the center of a positioning point and focusing on the rose red dye in the blood vessels of the cerebral cortex.

[0021] The imaging laser comprises:

[0022] It includes a femtosecond laser, which generates a 920nm laser and is incident on an imaging galvanometer group;

[0023] It includes a laser power controller to adjust the power of the femtosecond laser;

[0024] It includes an imaging galvanometer group, which controls the laser to focus on the target blood vessel segment after being modulated by the imaging galvanometer group to activate the fluorescein dextran dye and perform scanning imaging.

[0025] The blood vessel types of the cerebral cortical blood vessel object include cortical pial arteries and veins, small arteries and veins in the cortical parenchyma, or capillaries in the cortical parenchyma, with a blood vessel diameter ranging from 3 μm to 50 μm and a depth ranging from 0 to 800 μm.

[0026] The scanning imaging sequence is configured to consist of a stimulation-imaging sequence of 5 seconds in length that is continuously repeated, wherein the stimulation-imaging sequence of 5 seconds in length includes a scanning stimulation period of 3 seconds and an imaging period of 2 seconds.

[0027] During the 3-s scanning stimulation period, stimulation was performed using a stimulating femtosecond laser;

[0028] During the 2 s imaging period, imaging is performed using an imaging laser.

[0029] During the 3 s scanning stimulation period, the femtosecond laser in the stimulation femtosecond laser is controlled to generate continuous femtosecond laser in a repeated spiral scanning mode starting from the center of the positioning point, and the stimulation galvanometer group in the stimulation femtosecond laser is controlled to drive the femtosecond laser position to move for scanning.

[0030] The repeated spiral scanning mode starts from the center of the positioning point, specifically: the focus of the femtosecond laser emitted by the femtosecond laser is located at the positioning point at the target depth of the cerebral cortical blood vessels after the stimulation of the galvanometer group and the two-photon microscope system. The positioning point is the center of the region of interest ROI. The focal point is driven by the stimulation of the galvanometer group to perform uninterrupted repeated scanning along a planar spiral trajectory centered on the positioning point.

[0031] The single planar spiral trajectory scan is that the focus of the laser is modulated to start from the center of the selected region of interest ROI, move at a constant ultra-fast point scanning speed of 0.6μs / pixel in a continuous point scanning mode, and spiral outward along the planar spiral trajectory to the outer edge of the region of interest ROI at a step of 0.5μm / turn.

[0032] The wavelength of the near-infrared femtosecond laser is 1070nm, that is, 1070nm near-infrared femtosecond laser.

[0033] The laser power of the near-infrared femtosecond laser emitted from the two-photon microscope system was controlled to be 80-200 mW.

[0034] The region of interest ROI of the cerebral cortical blood vessels is a vascular cavity region within the vascular wall but not including the vascular wall.

[0035] In this way, the present invention uses a focused two-photon microscope system in combination with a 1070nm near-infrared femtosecond laser imaging laser to image and locate target vascular segments of cerebral cortical blood vessels labeled with fluorescein at different depths to obtain structural and blood flow conditions.

[0036] The present invention successfully achieves rapid, efficient and accurate vascular embolism simulation induction through the use of 1070nm near-infrared femtosecond laser, the combination of rose bengal reagent and the focusing treatment of the modulation method of repeated spiral scanning mode. It will not cause damage to the blood vessel wall and will not damage the blood-brain barrier. It avoids the problems of penetration depth influence, inaccuracy and imprecision caused by green light irradiation of about 500nm, as well as the problems of blood vessel wall damage and blood-brain barrier destruction caused by it.

[0037] The system of the present invention can simulate and induce vascular embolism, locate the target depth position very accurately, locate the capillaries accurately, and realize the high real-time requirement of real-time scanning and observation.

[0038] The rose bengal reagent can generate active oxygen free radicals under the stimulation of femtosecond laser, which can induce damage to vascular epithelial cells and cause platelet aggregation to produce embolism. Fluorescein dextran dye is used as a fluorescent imaging reagent to image the cortical vascular network structure under a two-photon system to observe the vascular network structure and blood flow.

[0039] This invention provides a practical, precise, and deeply selective single-vessel photothrombosis system using commercial optical equipment, providing an important tool for exploring neurovascular function in health and disease.

[0040] The present invention uses multi-photon imaging at intervals during the process of photothrombosis to obtain time series image data of vascular hemodynamics and structural changes in real time, and observes the vascular blood flow state in real time until thrombosis is formed.

[0041] The technology described in the present invention innovatively combines 1070nm near-infrared femtosecond laser stimulation with two-photon microscopy to achieve precise and rapid thrombosis of blood vessels of various types, sizes and depths, and is a new and more effective system for targeting vascular thrombosis formation.

[0042] Compared with the prior art, the present invention has the following advantages:

[0043] 1. High success rate and rapid thrombosis. The wavelength of 1070nm femtosecond laser is near the two-photon excitation peak of Rose Bengal, so it can effectively excite Rose Bengal; rapid and repeated spiral scanning can ensure rapid excitation of Rose Bengal in the target ROI, so the near-infrared femtosecond laser stimulation-induced vascular embolization technology has a very high thrombosis success rate and rapid thrombosis.

[0044] 2. Reliable, safe and low-damage. The point scanning method used when laser excites Rose Bengal and the two-photon excitation characteristics of Rose Bengal limit the light focusing and excitation range to the focal point, avoiding unnecessary excitation around the focal point; the ROI of spiral laser scanning is limited to the lumen of the blood vessel, which also avoids the photothermal and photodamage effects of the laser on the blood vessel wall cells; the alternating spiral scanning and imaging periods also promote the rapid dissipation of heat and reduce the impact of photothermal damage.

[0045] 3. Flexible operability and depth selectivity. The near-infrared femtosecond laser stimulation-induced vascular embolization technology uses commercial optical equipment to achieve thrombosis. The 1070nm laser is a commonly used and easily available laser. The 1070nm laser has excellent tissue penetration ability and less energy loss in the cortex. It can penetrate the cortex with a lower power laser to achieve deep vascular thrombosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 It is a specific implementation step diagram;

[0047] Figure 2 is a schematic diagram of a region of interest ROI and its planar spiral trajectory of the present invention;

[0048] Figure 3 is a schematic diagram of the thrombotic effect of the embodiment;

[0049] Figure 4 This is the comparison chart of the Rose Red experiment;

[0050] Figure 5 This is a comparison chart of spiral scanning methods. DETAILED DESCRIPTION

[0051] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0052] The system includes:

[0053] The method comprises a rose bengal reagent which is pre-injected into the cerebral cortical blood vessels to mark the plasma in the blood vessels to achieve photothrombosis. By pre-injecting the rose bengal reagent into the cerebral cortical blood vessels, the femtosecond laser irradiating the rose bengal reagent will excite fluorescence, thereby performing imaging.

[0054] It includes stimulating a femtosecond laser, generating a specific near-infrared femtosecond laser and injecting it into an optical lens of a two-photon microscope system;

[0055] Stimulation femtosecond lasers also include:

[0056] It includes a femtosecond laser, which generates near-infrared femtosecond laser and is incident on a galvanometer group;

[0057] It includes a stimulating galvanometer group, which controls the exit position of the laser after reflection or transmission modulation by the stimulating mirror group and then incident on the optical lens of the two-photon microscope system;

[0058] The device comprises a modulator connected to a stimulation galvanometer group. The modulator controls the modulation mode of the stimulation galvanometer group, and modulates a 1070nm near-infrared femtosecond laser into a repeated spiral scanning mode starting from the center of a positioning point and focusing on the cerebral cortical blood vessels.

[0059] It includes a two-photon microscope system, which focuses the femtosecond laser emitted by the stimulation femtosecond laser on the cerebral cortical blood vessels, and stimulates the region of interest ROI at the target depth of the cerebral cortical blood vessels to stimulate the formation of thrombus by rose bengal in the blood vessels; and processes the imaging beam generated by the imaging laser and focuses on the cerebral cortical blood vessels for imaging;

[0060] The imaging laser comprises an imaging laser, which generates an imaging beam incident on the two-photon microscope system; the imaging beam is usually a 920nm femtosecond laser.

[0061] Imaging lasers include:

[0062] It includes a femtosecond laser, which generates laser light and is incident on an imaging galvanometer group;

[0063] It includes an imaging galvanometer group, which controls the laser to focus on the target blood vessel segment and scan it after reflection or transmission modulation by the imaging galvanometer group.

[0064] The controller comprises a controller which is respectively connected with the stimulation femtosecond laser and the imaging laser to control the femtosecond laser in the stimulation femtosecond laser and the femtosecond laser in the imaging laser to work according to the scanning imaging sequence.

[0065] The scanning imaging sequence was set to consist of a 5-second stimulation-imaging sequence that was repeated continuously. The 5-second stimulation-imaging sequence included a 3-second scanning stimulation period and a 2-second imaging period.

[0066] During the 2 s imaging period, imaging is performed using an imaging laser.

[0067] During the 3s scanning stimulation period, the stimulation femtosecond laser is used for stimulation: the femtosecond laser in the stimulation femtosecond laser is controlled to generate continuous femtosecond laser in a repeated spiral scanning mode starting from the center of the positioning point, and the stimulation galvanometer group in the stimulation femtosecond laser is controlled to drive the femtosecond laser position to move for scanning.

[0068] In a repeated spiral scanning mode starting from the center of the positioning point, specifically: the femtosecond laser emitted by the femtosecond laser is focused at the positioning point at the target depth of the cerebral cortical blood vessels after passing through the stimulation galvanometer group and the optical lens of the two-photon microscope system. The positioning point is the center of the region of interest ROI. The focal point is driven by the stimulation galvanometer group to perform uninterrupted and repeated scanning along a planar spiral trajectory centered on the positioning point. The number of scans through the planar spiral trajectory within the 3s scanning stimulation period is 100 to 1000 times.

[0069] The single planar spiral trajectory scan is that the focus of the laser is modulated to start from the center of the selected region of interest ROI, move at a constant ultra-fast point scanning speed of 0.6μs / pixel in a continuous point scanning mode, and spiral outward along the planar spiral trajectory to the outer edge of the region of interest ROI at a step of 0.5μm / turn.

[0070] The laser power of the near-infrared femtosecond laser emitted from the optical lens of the two-photon microscope system is 80-200 mW.

[0071] In a specific implementation, the near-infrared femtosecond laser has a wavelength of 1070nm, a repetition frequency of 70MHZ, a pulse width of 55fs, and a laser power of 80-200mW after being emitted from the optical lens of the two-photon microscope system.

[0072] like Figure 3 As shown in the figure, the region of interest (ROI) of the cerebral cortical blood vessels is the vascular cavity area within the vascular wall, and cannot cover the vascular wall. The region of interest (ROI) is selected to be located within the maximum cross-section of the target vascular segment, with the edge close to the vascular wall and not covering the area outside the vascular cavity. All regions of interest (ROI) at different depths are selected based on the vascular structure and blood flow information collected by the two-photon microscopy system.

[0073] In this way, the present invention uses a focused two-photon microscope system in combination with a 1070nm near-infrared femtosecond laser imaging laser to image and locate target vascular segments of cerebral cortical blood vessels labeled with fluorescein at different depths to obtain structural and blood flow conditions.

[0074] like Figure 1 As shown, the working process of the present invention is as follows:

[0075] The method includes the injection of rose bengal reagent and fluorescein dextran dye into the cerebral cortical blood vessels in advance. Rose bengal is used to mark the plasma in the blood vessels to achieve photothrombosis; fluorescein dextran dye is injected into the cerebral cortical blood vessels, and the fluorescence is stimulated by femtosecond laser irradiation for imaging. The animals are equipped with imaging cranial windows and use a two-photon microscope with a tunable imaging femtosecond laser to record signals and collect data on vascular structure and blood flow information.

[0076] It includes a two-photon microscope system, which focuses the femtosecond laser emitted by the imaging femtosecond laser instrument on the cerebral cortical blood vessels at a specific depth. The imaging femtosecond laser generates laser light that is incident on the imaging galvanometer group, and after modulation, the laser is controlled to focus on the target blood vessel segment and scan. The blood vessels are imaged by exciting the dextran dye. The imaging beam is usually a 920nm femtosecond laser. Blood vessels at different depths in the cortex and with different sizes are located by two-photon imaging and the structure and blood flow information of the maximum cross section are obtained; the target blood vessel is selected based on the structure and blood flow information of the maximum cross section, and the region of interest ROI is set in the lumen of the target blood vessel, and the edge of the region of interest ROI is set to be tangent to the blood vessel wall and remain in the blood vessel lumen.

[0077] The method comprises a stimulating femtosecond laser, which generates a specific 1070nm near-infrared femtosecond laser and injects it into a two-photon microscope system. After being reflected and modulated by a stimulating mirror group, the scanning mode of the laser is controlled and then injected into the optical lens of the microscope system. A modulator controls the modulation mode of the stimulating galvanometer group, and modulates the 1070nm near-infrared femtosecond laser into a repeated spiral scanning mode starting from the center of a positioning point and focusing on the ROI. Stimulation is performed to stimulate the formation of thrombus by rose bengal in the blood vessel.

[0078] It includes using a main control computer to control the stimulation femtosecond laser and the stimulation scanning galvanometer group and the imaging femtosecond laser and the imaging scanning galvanometer group to work according to the scanning imaging sequence. The scanning imaging sequence is set to consist of a stimulation-imaging sequence with a duration of 5s that is continuously repeated, and the stimulation-imaging sequence with a duration of 5s includes a scanning stimulation period of 3s and an imaging period of 2s. During the 3s scanning stimulation period, the stimulation femtosecond laser is modulated at a specific power to start from the center of the positioning point of the region of interest ROI, and the laser focus moves at a constant ultra-fast point scanning speed of 0.6μs / pixel in a continuous point scanning mode, and spirals outward along a planar spiral trajectory to the end of the outer edge of the region of interest ROI at a step of 0.5μm / circle. During the scanning process, the laser scans repeatedly without interruption for more than 1000 times. After confirming the appearance of thrombus, the scanning sequence is terminated. In the specific implementation, the time from the start of scanning to the real-time observation of thrombus formation is the total duration of thrombus formation, which is controlled within 5 minutes.

[0079] In the specific implementation, the near-infrared femtosecond laser has a wavelength of 1070nm, a repetition frequency of 70MHZ, a pulse width of 55fs, and an optical lens of a two-photon microscope system, and controls the laser power after emission to be 80-200mW; the specific power value is adjusted with increasing depth

[0080] like Figure 2As shown in the figure, the region of interest (ROI) of the cerebral cortical blood vessels is the vascular cavity area within the vascular wall, and cannot cover the vascular wall. The region of interest (ROI) is selected to be located within the maximum cross-section of the target vascular segment, with the edge close to the vascular wall and not covering the area outside the vascular cavity. All regions of interest (ROI) at different depths are selected based on the vascular structure and blood flow information collected by the two-photon microscopy system.

[0081] like Figure 3 As shown, in a specific implementation, during the interval between adjacent 1070nm femtosecond laser stimulations, a multiphoton microscope is used in conjunction with an imaging instrument to perform time series imaging of the structure and blood flow of blood vessels in the cerebral cortex using a femtosecond laser, and observation is performed based on the time series imaging. The generated fluorescence is detected and imaged by a two-photon microscope system to observe the thrombosis situation and results in real time. When thrombosis is observed to be formed for more than 10 seconds, the scanning process is then stopped, and line scanning is used to confirm the blockage.

[0082] The experimental subjects in the specific implementation are rats, mice, cats and experimental monkeys, etc., but not limited to these.

[0083] Comparative Example 1 is as follows:

[0084] like Figure 4 As shown, the above-mentioned 1070nm stimulation femtosecond laser was used in combination with the 920nm imaging femtosecond laser to simulate the induction of vascular thrombosis. After scanning stimulation and imaging using the same scanning sequence parameters as mentioned above, no thrombosis was found in the blood vessel cavity without rose red marking, and the corresponding blood flow velocity of the blood vessel was not changed.

[0085] Comparative Example 2 is as follows:

[0086] like Figure 5 As shown, a 1070nm stimulating femtosecond laser was used and modulated into two scanning modes to simulate the induction of vascular thrombosis, one of which was a conventional scanning mode and the other was the spiral scanning mode mentioned above. The test was conducted on 46 blood vessels at the same depth of the cerebral cortex of different mice. The results showed that the spiral scanning mode had a significantly higher success rate and significantly faster thrombosis time than the conventional scanning mode, indicating the superiority of the spiral scanning mode.

[0087] Therefore, the present invention uses near-infrared laser stimulation in a multiphoton microscope to achieve precise and controllable thrombosis in blood vessels of various sizes with selectable depths in the cerebral cortex of living animals, providing a practical and accurate thrombosis model for cerebrovascular diseases, and providing a reference for the mechanism exploration and precise treatment of vascular diseases based on single vessels and focal lesions for studying neurovascular coupling mechanisms, cerebral small vessel diseases and neurodegenerative diseases.

Claims

1. A near-infrared femtosecond laser simulation-induced vascular embolism system, characterized in that the system include: Colorimetric reagents, including rose bengal reagent and fluorescein dextran dye, are pre-injected into the blood vessels of the cerebral cortex; Stimulate the femtosecond laser to generate specific near-infrared femtosecond laser and inject it into the two-photon microscope system; Imaging laser, which generates femtosecond laser light to be incident on the two-photon microscope system; The two-photon microscope system focuses the femtosecond laser emitted by the stimulation femtosecond laser on the region of interest below the target depth of the cerebral cortical blood vessels, and performs spiral scanning stimulation in the region of interest ROI to form a thrombus; the femtosecond laser emitted by the imaging laser is processed and focused on the cerebral cortical blood vessels for imaging; A controller, the controller is connected to the stimulation femtosecond laser and the imaging laser respectively, and controls the stimulation femtosecond laser and the imaging laser to work according to a scanning imaging sequence; The stimulation femtosecond laser further comprises: A femtosecond laser generates a 1070nm femtosecond laser which is incident on the galvanometer group; A laser power controller, which adjusts the power of the femtosecond laser emitted by the femtosecond laser; A stimulating mirror group, which controls the emission position of the laser after being modulated by the stimulating mirror group and then incident on the two-photon microscope system; A modulator is connected to the stimulation galvanometer group, and the modulator controls the modulation mode of the stimulation galvanometer group, modulating the near-infrared femtosecond laser to focus on the rose red dye in the blood vessels of the cerebral cortex in a repeated spiral scanning mode starting from the center of the positioning point; The imaging laser comprises: Femtosecond laser generates 920nm laser which is incident on the imaging galvanometer group; Laser power controller, which adjusts the power of femtosecond laser; An imaging galvanometer group, which controls the laser to focus on the target blood vessel segment after being modulated by the imaging galvanometer group to activate the fluorescein dextran dye and scan the image; The scanning imaging sequence is configured to consist of a stimulation-imaging sequence of 5 seconds in length that is continuously repeated, wherein the stimulation-imaging sequence of 5 seconds in length includes a scanning stimulation period of 3 seconds and an imaging period of 2 seconds. During the 3-s scanning stimulation period, stimulation was performed using a stimulating femtosecond laser; During the 2-s imaging period, imaging was performed using an imaging laser; The repeated spiral scanning mode starts from the center of the positioning point, specifically: the focus point of the femtosecond laser emitted by the femtosecond laser is located at the positioning point of the target depth of the cerebral cortical blood vessels after the femtosecond laser is stimulated by the galvanometer group and the two-photon microscope system. The positioning point is the center of the region of interest ROI, and the focus point is driven by the galvanometer group to perform uninterrupted repeated scanning along a planar spiral trajectory centered on the positioning point; The laser focus is modulated to start from the center of the selected region of interest (ROI) in a continuous point scanning mode at a constant ultra-fast point scanning speed of 0.6 μs / pixel, and spiral outward along the planar spiral trajectory to the outer edge of the region of interest (ROI) at a step of 0.5 μm / turn.

2. A near-infrared femtosecond laser simulation-induced vascular embolization system according to claim 1, characterized in that: The region of interest ROI of the cerebral cortical blood vessels is a vascular cavity region within the vascular wall but not including the vascular wall.

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