A blueberry soil conditioner and its preparation method and application

Abstract

The invention discloses a blueberry soil conditioner and a preparation method and application thereof. It belongs to the technical field of soil conditioners. The conditioner comprises the following components by mass: 95-99.5 parts of Bacillus coagulans waste liquid and 0.5-5 parts of component A; wherein the Bacillus coagulans waste liquid is obtained by centrifuging the fermented liquid of Bacillus coagulans after liquid fermentation; component A is one or more of fulvic acid, D-alanine and acetic acid. The beneficial effects of the present invention are as follows: adding a certain amount of component A to the Bacillus coagulans waste liquid can make the spores of Bacillus coagulans in the waste liquid continue to be in a dormant state, and at the same time can promote the growth of blueberries; after the soil is diluted and the conditioner is used, the spore germination inhibition factor disappears, the spores of Bacillus coagulans quickly wake up to reproduce and grow, and produce a large amount of acid, thereby achieving the purpose of regulating soil acidity; regulating soil acidity is sustainable, and the regulating effect can be maintained for 2 months after one application.

Description

Technical Field

[0001] The present invention relates to the technical field of soil conditioners, and more particularly to a blueberry soil conditioner and a preparation method and application thereof. Background Art

[0002] Bacillus coagulans is a Gram-positive bacterium belonging to the Firmicutes (or Thick-walled) phylum. Bacillus coagulans belongs to the genus Bacillus in taxonomy. The cells are rod-shaped, Gram-positive, with terminal spores and no flagella. It decomposes sugars to produce L-lactic acid and is a homolactic fermentation bacterium. The optimum growth temperature is 45-50°C, and the optimum pH is 6.6-7.0. When the pH value and water activity are less than 4.5 and 0.96 respectively, the growth of Bacillus coagulans will be inhibited to a certain extent. Bacillus coagulans waste liquid is a by-product of the production process of Bacillus coagulans. It is rich in nutrients such as protein, polysaccharides, and inorganic salts. It not only contains a large number of live Bacillus coagulans, but also contains small peptide antibacterial substances such as coagulins and L-lactic acid. These substances have a certain promoting effect on crop growth and a certain preventive effect on crop diseases. However, due to the difficulty in preservation and application value, the market demand is small, and it can generally only be treated as waste liquid, which increases the burden on environmental protection and enterprises. Therefore, without affecting the production efficiency of Bacillus coagulans, by controlling the centrifugation parameters, the number of live Bacillus coagulans in the waste liquid can be appropriately increased, and the live bacteria can be easier to preserve by adding substances that inhibit spore germination and appropriately adjusting the pH value of the liquid. At the same time, priority is given to adding fulvic acid, which has a good promoting effect on plant growth, as an acid-adjusting substance, to prepare a blueberry soil conditioner that can meet the blueberry soil conditioning needs. The waste liquid can be utilized, turning waste into treasure, which is green, environmentally friendly and low-carbon, and can also increase revenue for enterprises.

[0003] Blueberry is a perennial low shrub belonging to the genus Vaccinium of the Ericaceae family. Blueberry fruit is rich in nutrients, especially anthocyanins, which not only have good nutritional and health effects, but also have the functions of preventing brain nerve aging, strengthening the heart, anti-cancer, softening blood vessels, and enhancing human immunity.

[0004] Blueberries can only grow healthily in an acidic medium, and the pH value of the cultivation soil is most suitable for blueberry growth between 4 and 5. This problem has troubled many blueberry introducers, because the pH value of farmland soil in most areas is generally between 6.5 and 7.5, and the methods commonly used to adjust the soil pH value in the current introduction and planting are mainly to apply sulfur powder, wood vinegar, peat, acidic organic fertilizer, etc. Among them, the application of sulfur powder is the most economical, effective and more common, but because sulfur has a broad spectrum of insecticidal and fungicidal effects, the per mu dosage in general farmland soil must reach about 200 kg / mu to achieve the effect. While adjusting the acid, it will seriously affect the soil biological conditions, and thus affect the green cultivation of blueberries.

[0005] Therefore, providing a soil conditioner with continuous acid production capability and green environmental protection is the key to the successful introduction of blueberry green cultivation. Summary of the invention

[0006] In view of this, the present invention provides a blueberry soil conditioner and a preparation method and application thereof.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] A blueberry soil conditioner comprises the following components in parts by weight: 95 to 99.5 parts of Bacillus coagulans waste liquid and 0.5 to 5 parts of component A; wherein:

[0009] The waste liquid of Bacillus coagulans is obtained by centrifuging the fermented liquid of Bacillus coagulans and taking the supernatant;

[0010] The component A is one or more of fulvic acid, D-alanine and acetic acid.

[0011] The beneficial effects achieved are as follows: the component A has the function of inhibiting the germination of Bacillus coagulans spores and promoting the growth of blueberries, wherein the fulvic acid is not only a green crop growth promoter and stimulant, but also can be used as an acid-regulating substance, and D-alanine has a good inhibitory effect on the germination of Bacillus coagulans spores at a certain concentration.

[0012] Furthermore, a blueberry soil conditioner comprises the following components by weight: 95 parts of Bacillus coagulans waste liquid and 5 parts of component A; wherein,

[0013] The waste liquid of Bacillus coagulans is obtained by centrifuging the fermented liquid of Bacillus coagulans and taking the supernatant;

[0014] The component A is one or more of fulvic acid, D-alanine and acetic acid.

[0015] Furthermore, the component A is composed of fulvic acid and D-alanine, wherein the fulvic acid comprises 70 parts and the D-alanine comprises 30 parts.

[0016] Furthermore, the number of Bacillus coagulans spores in the Bacillus coagulans waste liquid is 4×10 6 ~9×10 6 Pieces / ml.

[0017] Furthermore, the pH value of the conditioning agent is adjusted to 3.8-4.5.

[0018] The preparation method of the above-mentioned blueberry soil conditioner comprises the following steps:

[0019] (1) After liquid fermentation of Bacillus coagulans, the fermentation liquid is centrifuged and the supernatant is taken to obtain Bacillus coagulans waste liquid;

[0020] The centrifugal parameters are: speed 14000r / min, processing capacity 15t / h;

[0021] (2) sterilizing component A at the following sterilization parameters: 0.15 MPa, 121° C., 50 to 60 min;

[0022] (3) The waste liquid of Bacillus coagulans and the sterilized component A are mixed evenly.

[0023] The above operations were all performed under sterile conditions.

[0024] Application of the above blueberry soil conditioner in blueberry planting.

[0025] Furthermore, the application method is to dilute the conditioner 40 to 100 times and then apply it to the roots of blueberries.

[0026] Furthermore, after watering, the soil moisture saturation at the roots of blueberries is controlled at 80-100%.

[0027] Furthermore, the conditioning agent is applied once every 45 to 70 days.

[0028] It can be seen from the above technical scheme that, compared with the prior art, the beneficial effects achieved by the present invention are as follows: (1) adding a certain amount of component A to the waste liquid of Bacillus coagulans can keep the spores of Bacillus coagulans in the waste liquid in a dormant state, while promoting the growth of blueberries; (2) after the soil is diluted and the conditioner is used, the factors inhibiting spore germination disappear, and the spores of Bacillus coagulans quickly wake up to reproduce and grow, and produce a large amount of acid, thereby achieving the purpose of regulating soil acidity; (3) the regulation of soil acidity is sustainable, and the regulation effect can be maintained for about 2 months after one application. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0030] Figure 1 The accompanying drawing is a comparison of the effects of the blueberry soil conditioner on regulating soil acidity in Experiment 1 of the present invention. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Other reagents required by the present invention are conventional experimental reagents, which are purchased from commercial channels; experimental methods not mentioned are conventional experimental methods and will not be described in detail here.

[0033] Example 1

[0034] Formula: 95 parts of Bacillus coagulans waste liquid and 5 parts of component A; wherein,

[0035] The waste liquid of Bacillus coagulans is obtained by centrifuging the fermented liquid of Bacillus coagulans and taking the supernatant;

[0036] Component A is fulvic acid.

[0037] Preparation method:

[0038] (1) After liquid fermentation of Bacillus coagulans (initial pH 6.50, culture conditions: 40°C, 150 rpm, ventilation volume 2 vvm, culture for about 60 h), the fermentation liquid is centrifuged and the supernatant is taken to obtain Bacillus coagulans waste liquid;

[0039] The centrifugal parameters are: speed 14000r / min, processing capacity 15t / h;

[0040] (2) sterilizing component A at the following sterilization parameters: 0.15 MPa, 121° C., 55 min;

[0041] (3) The waste liquid of Bacillus coagulans and the sterilized component A are mixed evenly.

[0042] Example 2

[0043] Formula: 99.5 parts of Bacillus coagulans waste liquid and 0.5 parts of component A; wherein,

[0044] The waste liquid of Bacillus coagulans is obtained by centrifuging the fermented liquid of Bacillus coagulans and taking the supernatant;

[0045] Component A is D-alanine.

[0046] The preparation method is the same as Example 1.

[0047] Experiment 1

[0048] Verify the effect of the conditioner prepared by the present invention on the soil of greenhouse blueberry field:

[0049] In order to investigate the effect of the conditioner prepared in Example 1 of the present invention on the acidity of greenhouse blueberry soil, a greenhouse where blueberries were to be planted six months later was selected for testing. Four treatments were designed with three repetitions. The plots were randomly arranged. Each plot had an area of ​​24 square meters. The initial pH value of the soil was 6.8. The soil was loamy. The treatment contents were as follows:

[0050] Treatment 1: control area, only conventional water management;

[0051] Treatment 2: Sulfur powder treatment area, application of sulfur powder + conventional water management;

[0052] Treatment 3: Inactivated blueberry soil conditioner treatment area, inactivated blueberry soil conditioner + conventional water management;

[0053] Treatment 4: Blueberry soil conditioner treatment area, application of blueberry soil conditioner + conventional water management.

[0054] Among them, conventional water management, full water irrigation was carried out on August 20, October 25, December 18 and February 15 when the soil was relatively dry, with a water consumption of about 40-50 kg / m2. Treatment 2 sulfur powder 190 kg / mu, applied at one time, mixed well and then watered; Treatment 3 and Treatment 4, the inactivated blueberry soil conditioner and blueberry soil conditioner were applied in batches, the first time 100L / mu, applied with water, the second and third times were used after 60 days and 120 days, 50L / mu. Times, and the same amount of water management was carried out for each treatment during the same period.

[0055] Soil samples were collected from each plot before the first, second, and third application and 180 days later to measure soil pH. The pH values ​​of each treatment at different time periods are shown in Table 1. Figure 1 As shown:

[0056] Table 1 Soil pH values ​​at different times in greenhouse soil acidification test using blueberry soil conditioner

[0057] August 20 October 25 December 18 February 15 Process 1 6.8 6.8 6.8 6.9 Process 2 6.8 4.4 4.6 4.8 Process 3 6.8 6.6 6.6 6.7 Process 4 6.8 5.7 5.1 4.9

[0058] From the above, it can be seen that in the past six months, after using blueberry soil conditioner three times, the soil pH value dropped to 4.9, which is basically equivalent to 4.8 in the sulfur powder treatment area, and fully meets the soil acidity requirements for blueberry planting.

[0059] Experiment 2

[0060] The effect of the conditioner prepared in Example 1 of the present invention on the soil of potted blueberries:

[0061] In order to investigate the effect of the conditioner prepared by the present invention on the soil acidity of potted blueberries, six potted blueberries with similar growth conditions were selected for soil acidification test. The test was set up with two treatments and three repetitions. The potted blueberries were not treated with acidification in the early stage. Treatment 1 was the control and normal water management was performed; Treatment 2 used 1 / 50 of the blueberry soil conditioner aqueous solution on August 20, October 25, and December 18, respectively, and water management was performed with reference to the water consumption of Treatment 1. Soil samples were collected from each pot before application to determine the soil pH value. The pH values ​​of each treatment at different time periods are as follows:

[0062] Table 2 Soil pH values ​​at different times in the blueberry soil conditioner acidification test for potted blueberries

[0063] August 20 October 25 December 18 February 5 Process 1 6.1 6.2 6.3 6.3 Process 2 6.1 5.5 4.9 4.4

[0064] From the above, we can see that in the past six months, after using blueberry soil conditioner three times, the soil pH value dropped from 6.1 to 4.4, while the pH value of the control soil without acidification treatment increased slightly to 6.3. At the same time, from the appearance of blueberry plants, treatment 2 is obviously better than treatment 1 in terms of growth and appearance.

[0065] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0066] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A blueberry soil conditioner, characterized in that: The method comprises the following components in parts by weight: 95-99.5 parts of waste liquid of Bacillus coagulans and 0.5-5 parts of component A; wherein, The waste liquid of Bacillus coagulans is obtained by centrifuging the fermented liquid of Bacillus coagulans and taking the supernatant; The component A is one or more of fulvic acid, D-alanine and acetic acid; The pH value of the conditioner is adjusted to 3.8-4.

5.

2. A blueberry soil conditioner according to claim 1, characterized in that: The invention comprises the following components by weight: 95 parts of waste liquid of Bacillus coagulans and 5 parts of component A; wherein, The waste liquid of Bacillus coagulans is obtained by centrifuging the fermented liquid of Bacillus coagulans and taking the supernatant; The component A is one or more of fulvic acid, D-alanine and acetic acid.

3. A blueberry soil conditioner according to claim 1, characterized in that: The number of Bacillus coagulans spores in the Bacillus coagulans waste liquid is 4×10 6 ~9×10 6 Pieces / ml.

4. The method for preparing the blueberry soil conditioner according to any one of claims 1 to 3, characterized in that: The steps include: (1) After liquid fermentation of Bacillus coagulans, the fermentation liquid is centrifuged and the supernatant is taken to obtain Bacillus coagulans waste liquid; The centrifugal parameters are: speed 14000r / min, processing capacity 15t / h; (2) Sterilize component A at the following parameters: 0.15 MPa, 121°C, 50-60 min; (3) Mix the waste liquid of Bacillus coagulans and the sterilized component A evenly.

5. Use of the blueberry soil conditioner according to any one of claims 1 to 3 in blueberry planting.

6. The use according to claim 5, characterized in that The application method is to dilute the conditioner by 40 to 100 times and then apply it to the roots of blueberries.

7. The use according to claim 6, characterized in that After watering, the soil moisture saturation at the roots of blueberries is controlled at 80~100%.

8. The use according to claim 7, characterized in that The conditioner is applied every 45 to 70 days.

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