Mixture consisting of phthalic acid, palmitic acid and azelaic acid and application thereof

Abstract

The present invention relates to a mixture consisting of phthalic acid, palmitic acid and azelaic acid. The present invention also relates to the use of the mixture (LCOA) in regulating the disease resistance of Panax notoginseng plants and / or repairing the continuous cropping soil of Panax notoginseng and / or improving the planting of Panax notoginseng. The present invention finds that by using the mixture (LCOA), the growth of root rot pathogens can be inhibited and the growth of dark green Trichoderma can be promoted. At the same time, by treating potted Panax notoginseng or soil in a root irrigation manner, the incidence of Panax notoginseng plants can be significantly reduced, the survival rate of Panax notoginseng plants can be improved, and the beneficial bacteria in the soil can be coordinated to alleviate the continuous cropping obstacles of Panax notoginseng, so as to facilitate the planting and growth of Panax notoginseng.

Description

Technical Field

[0001] The present invention relates to the technical field of Panax notoginseng planting, and in particular to the application of a mixture (LCOA) consisting of phthalic acid, palmitic acid and azelaic acid in regulating the disease resistance and survival rate of Panax notoginseng and repairing continuously cropped soil. Background Art

[0002] Panax notoginseng (Burk.) FHChen is a perennial herbaceous plant of the genus Panax in the Araliaceae family. It grows in warm and humid mountainous areas and is a precious Chinese medicinal material unique to my country. In my country, Panax notoginseng has a long history of medicinal use. The "Supplement to Compendium of Materia Medica" records: "Ginseng is the best for replenishing qi, and Panax notoginseng is the best for replenishing blood. They have the same taste and the same effect, so it is called ginseng Panax notoginseng, which is the most precious among Chinese medicines." Panax notoginseng is mainly used as a medicine with its roots and rhizomes. It has the functions of stopping bleeding and removing blood stasis, reducing swelling and relieving pain. Its main component, saponin, has a significant effect on the treatment of cardiovascular and cerebrovascular diseases. Panax notoginseng is not only an important medicinal material, but also a common food ingredient. Its flowers and leaves can be used as tea, and the root powder can also be used to make soup. Panax notoginseng is one of the characteristic industries of Yunnan. According to statistics, more than 90% of my country's Panax notoginseng comes from Yunnan Province. The production areas of Yunnan Panax notoginseng include Wenshan County, Yanshan County, Maguan, Xichou, Guangnan, Malipo, Funing, Qiubei, etc. The total area of ​​Panax notoginseng cultivation has exceeded 700,000 mu, with an annual output value of nearly 80 billion yuan.

[0003] However, there is a serious "taboo land" phenomenon in the production of Panax notoginseng. Due to the large-scale, single-pattern planting year after year, and the fact that Panax notoginseng is a perennial herb, the problem of continuous cropping obstacles has become increasingly prominent, seriously affecting the yield and quality of Panax notoginseng, and restricting the healthy development of the Panax notoginseng industry. Continuous cropping obstacles refer to the phenomenon that when the same crop is continuously planted on the same piece of land under normal cultivation methods and field management measures, the growth becomes weaker, the yield decreases, the quality decreases, and the spread of pests and diseases occurs. The causes of continuous cropping obstacles are very complex, and these main reasons can be summarized into the following five aspects: 1) Changes in the physical and chemical properties of the soil; 2) Deficiency of soil fertility and nutritional imbalance; 3) Intensification of soil-borne pests and diseases; 4) Accumulation of self-toxic substances; 5) Changes in soil microorganisms.

[0004] Several reasons that lead to the continuous cropping of Panax notoginseng during its cultivation process will cause an imbalance in the proportion of microorganisms in the soil. Bacteria and fungi are the dominant groups of soil microorganisms in the root zone of Panax notoginseng, and continuous cropping has a significant impact on the soil microbial flora. As the number of Panax notoginseng planting years increases, the number of bacteria decreases, and the diversity of microorganisms decreases, which is not conducive to the growth of Panax notoginseng.

[0005] Panax notoginseng root rot is also one of the most serious diseases affecting Panax notoginseng production, and it is one of the important reasons for the continuous cropping of Panax notoginseng. The pathogen of Panax notoginseng root rot is extremely complex. With the extension of the continuous cropping period of Panax notoginseng, the root rot becomes serious, and most of them are diseases of the underground part of the rhizome, which is difficult to prevent and control. Years of production practice have confirmed that root rot is a specific manifestation of continuous cropping obstacles. In production, the research on Panax notoginseng root rot only involves agricultural control, chemical control, biological control, etc. Due to the complexity of root rot and the limitations of each control measure, there has been no report on the promotion and application of effective control measures for Panax notoginseng root rot in the field, nor has there been any report on a single measure or a type of measure that can be more effectively controlled.

[0006] For a long time, the prevention and control of plant diseases has mainly relied on the selection and breeding of disease-resistant varieties and the use of chemical pesticides. However, the long-term and large-scale application of chemical synthetic fertilizers and pesticides has led to many problems such as agricultural ecological environment damage, reduced fertilizer and pesticide efficacy, and food safety. Therefore, seeking a new technology and method to increase the yield and protect Panax notoginseng has become an important research direction for agricultural science and technology workers around the world.

[0007] Yunnan Province is the main production area of ​​Panax notoginseng Chinese medicinal materials. According to the growth habits of Panax notoginseng, crop rotation in new land during the planting process of Panax notoginseng can reduce the harm of root rot to a certain extent. However, due to the long rotation cycle and the perennial crop rotation, the phenomenon of no land to plant in the main production areas of Panax notoginseng will be aggravated. In order to reduce production costs, reduce the harm of root rot, and alleviate the obstacles of continuous cropping, the present invention finds that the use of a mixture of phthalic acid, palmitic acid and azelaic acid (LCOA) can effectively improve the above problems and increase the survival rate of Panax notoginseng plants. Based on research, the present invention has found that a mixture containing phthalic acid, palmitic acid and azelaic acid (LCOA) has a very prominent effect in enhancing the disease resistance of Panax notoginseng plants and repairing the soil for continuous cropping of Panax notoginseng, and thus proposes the present invention. Summary of the invention

[0008] In order to alleviate the problem of continuous cropping obstacles of Panax notoginseng, improve the planting and growth environment of Panax notoginseng, and improve the quality of Panax notoginseng, the present invention provides a mixture (LCOA) comprising phthalic acid, palmitic acid and azelaic acid; the present invention also provides the use of the mixture (LCOA) comprising phthalic acid, palmitic acid and azelaic acid in regulating the disease resistance of Panax notoginseng plants and / or repairing the continuous cropping soil of Panax notoginseng and / or improving the planting of Panax notoginseng. The present invention also provides a method for using the mixture (LCOA) comprising phthalic acid, palmitic acid and azelaic acid to regulate the disease resistance of Panax notoginseng plants and / or repair the continuous cropping soil of Panax notoginseng and / or improve the planting of Panax notoginseng.

[0009] Among them: Phthalic acid, the molecular formula is C8H6O4. Its structure is as follows:

[0010]

[0011] Colorless flaky crystals. Melting point 234℃ (phthalic anhydride is generated when heated to above 200℃), relative density 1.593. Soluble in organic solvents such as methanol and ethanol, slightly soluble in water and ether, insoluble in chloroform. Used in synthetic resins, synthetic fibers and drugs, etc. Can be used to make dyes, polyester resins, polyester, drugs and plasticizers, etc. It can also be used as a chemical reagent and chromatographic analysis reagent, prepare standard solutions, and serve as alkali method standards. Verify cobalt, copper, germanium, mercury, nickel, potassium, strontium and zinc, and determine cobalt, iodate and lead, etc.

[0012] Palmitic acid, molecular formula: C16H32O2. Its structure is as follows:

[0013]

[0014] It is white scale with pearlescent luster, also known as palmitic acid, scientific name hexadecanoic acid. It exists in the form of glyceride in many oils and fats. It can be directly extracted from palm fruit or produced by hydrolysis of palm oil. It is insoluble in water, slightly soluble in cold alcohol and petroleum ether, soluble in hot ethanol, ether and chloroform, etc. Palmitic acid is a long-chain saturated fatty acid that not only maintains the structure and integrity of cell membranes, but also plays an important role in cell signal transmission and energy storage. It can be used to make candles, soaps, lubricants, synthetic detergents, softeners, etc. Palmitic acid also has a positive significance in promoting plant growth and inhibiting pathogenic microorganisms.

[0015] Azelaic acid has the molecular formula: C9H 14 O4, whose structure is as follows:

[0016]

[0017] Commonly known as azelaic acid, it is colorless to light yellow crystals or crystalline powders in appearance and properties. Its medium-length carbon chain plus two carboxyl groups give azelaic acid a wide range of applications. It is slightly soluble in cold water, soluble in hot water and ether, and easily soluble in ethanol. It can be used as a plasticizer and in alkyd resins, paints and chemical synthesis. Azelaic acid also has antibacterial properties and can be used as a food preservative. Using it in mouthwashes is beneficial for the prevention and treatment of dental caries, using it in soaps can avoid cracking on the soap surface, and it has good permeability to the skin. Using it in creams and cosmetics can increase the skin's absorption function.

[0018] The present invention finds that a mixture (LCOA) composed of phthalic acid, palmitic acid and azelaic acid can inhibit the growth of root rot pathogens, enhance the biocontrol function of the biocontrol bacterium Trichoderma harzianum, reduce the incidence of Panax notoginseng plants, improve the survival rate of Panax notoginseng plants, and alleviate the problem of root rot in Panax notoginseng. Therefore, the mixture (LCOA) containing phthalic acid, palmitic acid and azelaic acid in the application and method of the present invention can inhibit the growth of root rot pathogens and promote the growth of Trichoderma harzianum. The mixture (LCOA) containing phthalic acid, palmitic acid and azelaic acid in the application and method of the present invention can alleviate the problem of root rot in Panax notoginseng.

[0019] An object of the present invention is to provide a mixture (LCOA) consisting of phthalic acid, palmitic acid and azelaic acid.

[0020] Another object of the present invention is to provide a mixture of phthalic acid, palmitic acid and azelaic acid (LCOA) for use in regulating the disease resistance of Panax notoginseng plants and / or repairing the soil for continuous cropping of Panax notoginseng and / or improving Panax notoginseng planting.

[0021] Another object of the present invention is to provide a method for regulating the disease resistance of Panax notoginseng plants and / or repairing the soil for continuous cropping of Panax notoginseng and / or improving Panax notoginseng cultivation using a mixture of phthalic acid, palmitic acid and azelaic acid (LCOA).

[0022] In a preferred embodiment, the regulating disease resistance of Panax notoginseng plants of the present invention includes enhancing the disease resistance of Panax notoginseng plants and improving the survival rate; the disease resistance includes resistance to Panax notoginseng black spot disease and root rot. The repairing of Panax notoginseng continuous cropping soil of the present invention includes reducing the obstacles of Panax notoginseng continuous cropping. The improving of Panax notoginseng planting of the present invention includes improving the survival rate of Panax notoginseng during planting.

[0023] Preferably, in the use and method of the present invention, the mixture (LCOA) can be prepared into a suitable concentration, for example, with water, when used. Preferably, the concentration is 0.01 to 100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 50 μg / mL, 100 μg / mL, and more preferably 0.01 to 10 μg / mL.

[0024] Preferably, in the application and method of the present invention, a mixture (LCOA) is used for root irrigation when regulating the disease resistance of Panax notoginseng plants.

[0025] In a preferred embodiment, the application and method include root irrigation of Panax notoginseng plants using the mixture (LCOA). Preferably, the roots can be left for a period of time after root irrigation, and the time is preferably more than 6 hours, or more than 12 hours, or more than 24 hours. The concentration of the mixture (LCOA) is 0.01 to 100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 50 μg / mL, 100 μg / mL, and more preferably 0.01 to 10 μg / mL.

[0026] The present invention also provides a method for regulating the disease resistance of Panax notoginseng plants and / or improving Panax notoginseng planting, the method comprising using a mixture (LCOA) consisting of phthalic acid, palmitic acid and azelaic acid for root irrigation.

[0027] In a preferred embodiment, the use and method include administering biocontrol bacteria, especially Trichoderma harzianum, while using the mixture (LCOA) for root irrigation. The concentration of the mixture (LCOA) is 0.01-100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 50 μg / mL, 100 μg / mL, more preferably 0.01-10 μg / mL.

[0028] The present invention also provides a method for repairing Panax notoginseng continuous cropping soil, which comprises treating the Panax notoginseng continuous cropping soil with a mixture of phthalic acid, palmitic acid and azelaic acid (LCOA).

[0029] Preferably, in the use and method of the present invention, the mixture (LCOA) can be prepared into a suitable concentration, for example, with water, when used. Preferably, the concentration is 0.01 to 100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 50 μg / mL, 100 μg / mL, and more preferably 0.01 to 10 μg / mL.

[0030] In a preferred embodiment, the use and method include administering biocontrol bacteria, especially dark green Trichoderma harzianum, while treating the soil of Panax notoginseng continuous cropping with the mixture (LCOA). The concentration of the mixture (LCOA) is 0.01-100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 50 μg / mL, 100 μg / mL, and more preferably 0.01-10 μg / mL.

[0031] In the present invention, the dosage of the mixture (LCOA) is 0.5 μg, 5.0 μg, 50 μg, 500 μg, 100 μg, 1500 μg, 2500 μg, 5000 μg per pot of Panax notoginseng, and more preferably 0.5-500 μg.

[0032] The mixture (LCOA) of the present invention can be applied to farmland, especially Panax notoginseng continuous cropping farmland to regulate the disease resistance of Panax notoginseng plants and / or repair Panax notoginseng continuous cropping soil and / or improve Panax notoginseng planting.

[0033] In the present invention, the "mixture (LCOA)" is a mixture of phthalic acid, palmitic acid and azelaic acid; preferably, the mass ratio of phthalic acid, palmitic acid and azelaic acid in the mixture is 1-3:1-3:1-3, preferably (1, 2 or 3):(1, 2 or 3):(1, 2 or 3); further preferably 1-2:1-2:1-2, more preferably 1:1:1; in the solution, its mass concentration ratio is 1-3:1-3:1-3, preferably (1, 2 or 3):(1, 2 or 3):(1, 2 or 3); further preferably 1-2:1-2:1-2, more preferably 1:1:1.

[0034] In the present invention, the mixture can be prepared with auxiliary materials into aqueous solution, aqueous solvent, drops, emulsifiable concentrate, microemulsion, aqueous emulsion, microcapsule, aerosol, spray and other dosage forms. Preferably, the mixture can be prepared with water to a suitable concentration when used.

[0035] In the present invention, the dark green Trichoderma is dark green Trichoderma (Trichoderma harzianum) T21-2, and the strain preservation number is CGMCC No.22462, which has been disclosed in CN202210325832.5.

[0036] In the present invention, unless otherwise specified, the Panax notoginseng plants generally refer to Panax notoginseng plants during the planting period, especially one-year-old Panax notoginseng plants.

[0037] Beneficial effects:

[0038] The present invention uses a mixture of phthalic acid, palmitic acid and azelaic acid to treat potted Panax notoginseng and soil in a watering manner, and analyzes the function of the mixture of phthalic acid, palmitic acid and azelaic acid in the field planting process of Panax notoginseng from the perspective of soil microorganisms. The present invention finds that after watering the Panax notoginseng potted plants with the mixture of phthalic acid, palmitic acid and azelaic acid, the survival rate of Panax notoginseng increases and the incidence of black spot disease decreases; the mixture of phthalic acid, palmitic acid and azelaic acid can inhibit the growth of root rot pathogens and promote the growth of dark green Trichoderma. After watering the potted Panax notoginseng with a mixture of phthalic acid, palmitic acid and azelaic acid and soil beneficial bacteria such as dark green Trichoderma, the survival rate of Panax notoginseng increases and the incidence of root rot disease decreases; by preparing a certain concentration of phthalic acid, palmitic acid and azelaic acid mixture to water the Panax notoginseng continuous cropping soil, the adaptability of Panax notoginseng to the continuous cropping soil can be significantly regulated, so that the Panax notoginseng in the continuous cropping soil can grow normally. At the same time, compared with the monomers, the mixture of the three has a lower concentration and more stable effect on root rot pathogens and dark green Trichoderma. Phthalic acid, palmitic acid, and azelaic acid are all easily available and can be degraded by nature without causing environmental pollution. Therefore, they can be widely used in the field cultivation of Panax notoginseng. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 :Effects of phthalic acid, palmitic acid and azelaic acid monomers on the growth of dark green Trichoderma T21-2 and root rot pathogen RS6;

[0040] Figure 2 :Effects of a mixture of phthalic acid, palmitic acid and azelaic acid on the growth of Trichoderma viride T21-2 and root rot pathogen RS6;

[0041] Figure 3 : Effect of a mixture of phthalic acid, palmitic acid and azelaic acid on the resistance of Panax notoginseng to black spot disease;

[0042] Figure 4 :Effects of a mixture of phthalic acid, palmitic acid and azelaic acid in combination with dark green Trichoderma T21-2 on the incidence, survival rate and root dry weight of Panax notoginseng;

[0043] Figure 5 :The effect of the mixture of phthalic acid, palmitic acid and azelaic acid on the survival rate and incidence of Panax notoginseng by inhibiting the root rot pathogen RS6;

[0044] Figure 6 : Effect of a mixture of phthalic acid, palmitic acid and azelaic acid on the survival rate of Panax notoginseng seedlings in continuously cropped soil.

[0045] Among them, different lowercase letters indicate significant differences at the P < 0.05 level tested by Duncan's new multiple range method. DETAILED DESCRIPTION

[0046] The present invention is described in more detail hereinafter to facilitate understanding of the present invention.

[0047] It should be understood that the terms or words used in the specification and claims should not be understood as having the meaning defined in the dictionary, but should be understood as having the meaning consistent with their meaning in the context of the present invention based on the following principle: the concept of the term can be appropriately defined by the inventor for the best explanation of the present invention.

[0048] Example 1: Effects of Phthalic Acid, Palmitic Acid and Azelaic Acid Monomers on the Growth of Trichoderma viridis and Root Rot Pathogens

[0049] Phthalic acid, azelaic acid and palmitic acid analytically pure monomers were selected and prepared at different concentrations and added to the PDA culture medium. The final concentrations were 0, 0.01, 0.1, 1, 10, and 100 μg / mL. After sterilization, PDA plates were prepared for use. In the clean bench, the root rot pathogen (Ilyonectria destructans) Rs6 and dark green Trichoderma (Trichoderma harzianum) T21-2 were made into 6 mm bacterial cakes using a hole puncher. The bacterial cakes were inoculated in the middle of the above culture medium, one in each dish, and 4 replicates were used for each treatment. The culture medium containing 1% methanol was used as the control. After inoculation, the colony diameter was measured after culturing in the dark at 25°C for 3-5 days, and the hyphae inhibition rate was calculated. The results are shown in Figure 1 middle.

[0050] The inhibition rate formula is as follows:

[0051] Inhibition rate (%) = [(control colony radius - confrontation culture colony radius) / control colony radius] × 100%

[0052] according to Figure 1 It can be seen that phthalic acid and palmitic acid monomers can significantly promote the growth of dark green Trichoderma at a concentration of 0.01-100 μg / mL, and can promote the growth of dark green Trichoderma at a concentration of 0.01-1 μg / mL; all three substances can inhibit the growth of root rot pathogens, and there are significant differences only at 1 and 10 μg / mL.

[0053] Example 2: Effect of LCOA on the growth of Trichoderma viride and root rot pathogens

[0054] The pure substances of phthalic acid, azelaic acid and palmitic acid were selected for analysis and added to PDA culture medium with different concentrations. The final concentrations were 0, 0.01, 0.1, 1, and 10 μg / mL (the mass concentration ratio of phthalic acid, palmitic acid and azelaic acid: 1:1:1). After sterilization, PDA plates were prepared for use. The antibacterial activity was determined by the growth rate method. In an ultra-clean workbench, the root rot pathogen (Ilyonectria destructans) Rs6 and dark green Trichoderma (Trichoderma harzianum) T21-2 were made into 6 mm bacterial cakes with a puncher. The bacterial cakes were inoculated in the middle of the above culture medium, one in each dish, and each treatment was repeated 4 times. The culture medium containing 1% methanol was used as the control. After inoculation, the plates were cultured at 25°C in the dark for 3-5 days, and the mycelium inhibition rate was calculated. The results are shown in Figure 2 middle.

[0055] The inhibition rate formula is as follows:

[0056] Inhibition rate (%) = [(control colony radius - confrontation culture colony radius) / control colony radius] × 100%

[0057] according to Figure 2 It can be seen that the mixture of phthalic acid, palmitic acid and azelaic acid (LCOA) at different concentrations can significantly inhibit the growth of root rot pathogens and promote the growth of dark green Trichoderma. Comparing it with Example 1, it can be seen that the mixture of phthalic acid, palmitic acid and azelaic acid (LCOA) can significantly affect the growth of root rot pathogens and dark green Trichoderma at a lower concentration, and compared with the monomers, the mixture of the three has a lower concentration and a more stable effect on root rot pathogens and dark green Trichoderma.

[0058] Example 3: Experiment on inoculation of leaves of Panax notoginseng after LCOA treatment

[0059] This experiment adopted the potted root irrigation method: LCOA solution was irrigated into the roots of Panax notoginseng potted plants according to the treatment gradient of final concentration of 0, 0.01, 0.1, 1, 10μg / mL. The solution preparation method used was as follows: 0.1g of LCOA sample was accurately weighed with an electronic balance and poured into a centrifuge tube (15mL) containing 10mL of methanol, and the solution was fully mixed to a dilution concentration of 10mg / mL. 1mL of the 10mg / mL dilution solution was drawn into a centrifuge tube (15mL) containing 9mL of methanol with a dilution of 1mg / mL using a pipette. The dilution was 0.1mg / mL and 0.01mg / mL in turn according to this method. Then 10mL of the mixed solution at the corresponding concentration was taken and added to a measuring cylinder containing 1000mL of water to prepare the required 1μg / mL, 0.1μg / mL, and 0.01μg / mL target solutions (mass concentration ratio of phthalic acid, palmitic acid and azelaic acid: 1:1:1). 1% methanol was used as the control treatment. Each group was treated with 8 replicates, and each replicate was watered with 50 mL of the diluted LCOA solution. After 24 hours, the leaves were inoculated with fungi (the inoculated fungus was the pathogen of Panax notoginseng black spot disease SL17Alternaria panax), and the leaves were kept moisturized until the disease occurred. The lesion area was investigated and the lesion area was scanned with a corresponding scanner and calculated using Adobe Photoshop CS6. Figure 3 It can be seen that the area of ​​leaf lesions of Panax notoginseng plants planted in continuous soil after root irrigation with LCOA solution was reduced, and the best effect of inhibiting pathogens was achieved when the LCOA concentration was 0.01μg / mL, and the disease resistance effect was second when the LCOA concentration was 0.1μg / mL and 1μg / mL. The overall trend was obvious and the effect was significant.

[0060] Example 4: Experiment on the effect of LCOA and dark green Trichoderma T21-2 on the survival rate and incidence of Panax notoginseng

[0061] This experiment adopted the potted root irrigation method: the Panax notoginseng potted plants were placed in a greenhouse, and all soils were sterilized soil. The solution preparation method used was: 0.1g of LCOA sample was accurately weighed with an electronic balance and poured into a centrifuge tube (15mL) containing 10mL of methanol, and the solution was fully mixed to a dilution concentration of 10mg / mL. 1mL of the 10mg / mL dilution solution was drawn into a centrifuge tube (15mL) containing 9mL of methanol with a pipette, and the dilution was 1mg / mL. According to this method, dilutions of 0.1mg / mL and 0.01mg / mL were prepared in turn, and 10mL of the mixed solution at the corresponding concentration was taken and added to a measuring cylinder containing 1000mL of water to prepare the required 10μg / mL, 1μg / mL, 0.1μg / mL, and 0.01μg / mL target solutions (mass concentration ratio of phthalic acid, palmitic acid and azelaic acid: 1:1:1); 1% methanol was used as a control treatment. Each group was treated with 6 replicates, for a total of 30 pots. 200 mL of mixed solution (50 mL LCOA + 50 mL Trichoderma harzianum T21-2 solution + 100 mL water) was added to each pot. 0 μg / mL (50 ml of 1% methanol + 50 mL Trichoderma harzianum T21-2 solution + 100 mL water) represents the control treatment, which uses 0 μg / mL LCOA for root irrigation. The survey items are the number of seedlings, fresh and dry weight, and incidence rate. The calculation formula is as follows:

[0062] Plant morbidity rate (%) = (number of diseased plants / total number of plants) × 100%

[0063] Plant survival rate (%) = (number of surviving plants / total number of plants) × 100%

[0064] The results are shown in Figure 4 middle.

[0065] according to Figure 4 It can be seen that after watering Panax notoginseng with LCOA+T21-2 solution with a concentration of 0.01-10 μg / mL, the incidence rate of Panax notoginseng plants was reduced, and the trend was obvious; the seedling survival rate and root dry weight were significantly increased.

[0066] Example 5: Experiment on the effect of LCOA on the survival rate and incidence of Panax notoginseng by inhibiting root rot pathogen RS6

[0067] This experiment adopted the potted root irrigation method: the Panax notoginseng potted plants were placed in a greenhouse, and all soils were sterilized soil. The solution preparation method used was: 0.1g of LCOA sample was accurately weighed with an electronic balance and poured into a centrifuge tube (15mL) containing 10mL of methanol, and the solution was fully mixed to a dilution concentration of 10mg / mL. 1mL of the 10mg / mL dilution solution was drawn into a centrifuge tube (15mL) containing 9mL of methanol with a pipette, and the dilution was 1mg / mL. According to this method, dilutions of 0.1mg / mL and 0.01mg / mL were prepared in turn, and 10mL of the mixed solution at the corresponding concentration was taken and added to a measuring cylinder containing 1000mL of water to prepare the required 10μg / mL, 1μg / mL, 0.1μg / mL, and 0.01μg / mL target solutions (mass concentration ratio of phthalic acid, palmitic acid and azelaic acid: 1:1:1); 6 replicates were set, with a total of 30 pots. Add 200mL of mixed solution (50mL LCOA + 50mL RS6 solution of root rot pathogen (Ilyonectria destructans) + 100mL water) to each potted plant, and conduct regular surveys after root irrigation. The survey items are the number of seedlings, fresh and dry weight, and incidence rate. 0μg / ml (50mL 1% methanol + 50mL RS6 solution of root rot pathogen (Ilyonectria destructans) + 100mL water) represents the control treatment, that is, 0μg / mL LCOA is used for root irrigation. The results are shown in Figure 5 middle.

[0068] The calculation formula is as follows:

[0069] Plant morbidity rate (%) = (number of diseased plants / total number of plants) × 100%

[0070] Plant survival rate (%) = (number of surviving plants / total number of plants) × 100%

[0071] according to Figure 5 It can be seen that after watering Panax notoginseng with LCOA+RS6 solution at a concentration of 0.01μg / mL-10μg / mL, the incidence of Panax notoginseng plants decreased, and the trend was obvious. The incidence was lowest when the concentration was 0.1μg / mL, and the corresponding seedling survival rate was also significantly improved. The underground seedling survival rate increased significantly under different concentration treatments, and the effect was obvious.

[0072] Example 6: Experiment on the effect of LCOA treatment on the survival rate and incidence of Panax notoginseng in continuous cropping soil

[0073] This experiment uses the potted root irrigation method: the continuous cropping soil is placed in the potted plants, and then 50mL of LCOA solution is used for irrigation according to the treatment gradient of 0μg / mL (10mL methanol + 1L water), 0.01μg / mL, 0.1μg / mL, and 1μg / mL. The solution preparation method used is: 0.1g of LCOA sample is accurately weighed with an electronic balance and poured into a centrifuge tube (15mL) containing 10mL methanol, and the solution is fully mixed to a dilution concentration of 10mg / mL. Use a pipette to draw 1 mL from the 10 mg / mL dilution solution into a centrifuge tube (15 mL) containing 9 mL of methanol, and the dilution is 1 mg / mL. According to this method, dilutions of 0.1 mg / mL and 0.01 mg / mL are prepared in turn. Then 10 mL of the mixed solution at the corresponding concentration is added to a measuring cylinder containing 1000 mL of water to prepare the required 10 μg / mL, 1 μg / mL, 0.1 μg / mL, and 0.01 μg / mL target solutions (the mass concentration ratio of phthalic acid, palmitic acid, and azelaic acid: 1:1:1); 10 mL of methanol is added to 1000 mL of water to prepare a 1% methanol solution; 50 mL of LCOA solution is taken from each concentration to irrigate the continuous cropping soil in the flowerpot, and the Panax notoginseng seedlings are transplanted after three days of bagging for moisture retention. Each group is treated with 12 replicates, a total of 60 pots. The survival rate and biomass of Panax notoginseng plants were investigated. 1% methanol solution represents the control treatment, i.e., 0 μg / mL LCOA was used for root irrigation. The results are shown in Figure 6 middle.

[0074] The calculation formula is as follows: Plant incidence rate (%) = (number of diseased plants / total number of plants) × 100%

[0075] Plant survival rate (%) = (number of surviving plants / total number of plants) × 100%

[0076] according to Figure 6 It can be seen that the survival rate of Panax notoginseng plants planted in the continuous cropping soil after irrigating with LCOA was improved. The survival rate of Panax notoginseng under irrigating with 0.01μg / mL LCOA reached 66.7%, an increase of 19.9% ​​compared with the control; the survival rate of Panax notoginseng under irrigating with 0.1μg / mL and 1μg / mL LCOA increased by 17.8% compared with the control, and the survival rate of Panax notoginseng under 10μg / mL LCOA treatment increased by 22%; overall, the trend was obvious and the effect was significant.

[0077] The above describes the preferred embodiments of the present invention, but it is not intended to limit the present invention. Those skilled in the art may make improvements and changes to the embodiments disclosed herein without departing from the scope and spirit of the present invention.

Claims

1. A mixture of phthalic acid, palmitic acid and azelaic acid, characterized in that: The mass ratio of phthalic acid, palmitic acid and azelaic acid in the mixture is 1:1:

1.

2. Use of the mixture according to claim 1 in regulating disease resistance of Panax notoginseng plants and / or repairing soil for continuous cropping of Panax notoginseng and / or improving Panax notoginseng planting.

3. The use according to claim 2, characterized in that: The mixture is prepared into a suitable concentration when used; the concentration is 0.01-100 μg / mL.

4. The use according to claim 2, characterized in that: The mixture is prepared into a suitable concentration when used; the concentration is 0.01-10 μg / mL.

5. The use according to claim 3, characterized in that: When used, the mixture is prepared with water to a suitable concentration.

6. The use according to any one of claims 2 to 5, characterized in that: When regulating the disease resistance of Panax notoginseng plants, use the mixture for root irrigation.

7. The use according to claim 6, characterized in that: The application includes administering biocontrol bacteria simultaneously with root irrigation using the mixture.

8. The use according to claim 7, characterized in that: The application involves administering Trichoderma harzianum simultaneously with the root irrigation using the mixture.

9. A method for regulating disease resistance of Panax notoginseng plants and / or repairing Panax notoginseng continuous cropping soil and / or improving Panax notoginseng planting using the mixture according to claim 1.

10. The method according to claim 9, characterized in that The mixture is prepared into a suitable concentration when used; the concentration is 0.01-100 μg / mL.

11. The method according to claim 9, characterized in that The mixture is prepared into a suitable concentration when used; the concentration is 0.01-10 μg / mL.

12. The method according to claim 10, characterized in that When used, the mixture is prepared with water to a suitable concentration.

13. The method according to any one of claims 9 to 12, characterized in that: When regulating the disease resistance of Panax notoginseng plants, use the mixture for root irrigation.

14. The method according to claim 13, characterized in that The method comprises administering biocontrol bacteria simultaneously when using the mixture for root irrigation.

15. The method according to claim 14, characterized in that The method comprises administering Trichoderma harzianum simultaneously with root irrigation using the mixture.

16. A method for regulating disease resistance of Panax notoginseng plants and / or improving Panax notoginseng planting, the method comprising using the mixture according to claim 1 for root irrigation.

17. The method according to claim 16, characterized in that The method comprises administering biocontrol bacteria simultaneously when using the mixture for root irrigation.

18. The method according to claim 17, characterized in that The method comprises administering Trichoderma harzianum simultaneously with root irrigation using the mixture.

19. A method for remediating Panax notoginseng continuous cropping soil, the method comprising treating the Panax notoginseng continuous cropping soil with the mixture according to claim 1.

20. The method according to claim 19, characterized in that The mixture is prepared into a suitable concentration when used; the concentration is 0.01-100 μg / mL.

21. The method according to claim 19, characterized in that The mixture is prepared into a suitable concentration when used; the concentration is 0.01-10 μg / mL.

22. The method according to any one of claims 19 to 21, characterized in that: When using the mixture to treat the soil of continuous cropping of Panax notoginseng, biocontrol bacteria are also given at the same time.

23. The method according to claim 22, characterized in that When the mixture was used to treat the soil of continuous cropping of Panax notoginseng, dark green Trichoderma harzianum was also administered simultaneously.

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