A phenothiazine-based near-infrared lipid droplet fluorescent dye, a preparation method and application thereof

By using phenothiazine-based near-infrared lipid droplet fluorescent dyes to emit near-infrared light in a low-polarity environment, the problems of short wavelength and poor stability in existing technologies have been solved, enabling efficient and specific imaging and dynamic analysis of lipid droplets in vivo.

CN117069678BActive Publication Date: 2026-04-07HUBEI ENG UNIV
0 Cites 0 Cited by

Patent Information

Application Number
CN202310824198.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-07-06
Publication Date
2026-04-07
Estimated Expiration
2043-07-06

AI Technical Summary

Technical Problem

Existing lipid droplet fluorescent dyes have short emission wavelengths and poor photostability, making them unsuitable for in vivo in situ imaging and lipid droplet dynamic analysis, and they also lack specificity.

Method used

A near-infrared lipid droplet fluorescent dye based on phenothiazine was developed, which emits near-infrared light in a low-polarity environment through a specific chemical structure, exhibiting a large Stokes shift and high fluorescence intensity. The preparation method includes reaction and purification in an anhydrous polar solvent.

Benefits of technology

It enables specific imaging of lipid droplets within living cells, allowing analysis of droplet division and lipid migration behavior, and exhibits significant resistance to photobleaching, making it suitable for in situ in vivo imaging.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

This invention discloses a phenothiazine-based near-infrared lipid droplet fluorescent dye, its preparation method, and its applications, belonging to the field of fluorescence sensing and detection technology. The phenothiazine-based near-infrared lipid droplet fluorescent dye is compound II with the following structural formula. In a low-polarity environment, the emission wavelength of this phenothiazine-based near-infrared lipid droplet fluorescent dye is located in the near-infrared region, exhibiting a large Stokes shift and strong fluorescence intensity. It can be used for specific imaging of lipid droplets within living cells, demonstrating strong specificity for lipid droplets and enabling imaging analysis of intracellular lipid droplet division and lipid migration. Leveraging the advantages of near-infrared emission, this dye can be used for in situ imaging of mouse breast cancer. Furthermore, the near-infrared lipid droplet fluorescent dye of this invention exhibits strong stability and significantly superior photobleaching resistance compared to Nile Red.
Need to check novelty before this filing date? Find Prior Art