A wax sealing composition for wax sealing storage of seeds and its application

Abstract

The invention discloses a wax sealing composition for wax sealing storage of seeds and its application. The wax sealing composition is prepared by mixing 16 to 20 parts of carboxymethyl chitosan, 6 to 10 parts of polyvinyl alcohol, 6 to 14 parts of propylene glycol and 11 to 13 parts of glycerol by mass. The wax sealing composition can be used to prepare wax sealing liquid. After the seeds are dried, the dried seeds are dipped in wax with the wax sealing liquid to ensure that the surface of the seeds is all thinly waxed. After being dispersed and dried in the shade, they are placed in a ventilated, dry and light-proof place indoors for storage at room temperature or low temperature, which can extend the storage time of the seeds and maintain the vitality of the seeds. The invention provides a new energy-saving method for seed storage, which is simple, low-cost and has good effect in the actual storage of Gaozhou tea oil seeds. The invention conforms to the new idea of ​​the country to promote sustainable agricultural development, and the wax sealing composition has broad application prospects in the actual storage of seeds.

Description

Technical Field

[0001] The invention relates to the technical field of seed storage, and more particularly to a wax sealing composition for seed wax sealing storage and application thereof. Background Art

[0002] Seed Storage Technique is a technology that uses reasonable storage equipment and advanced scientific methods to store seeds. By artificially controlling storage conditions, the change in seed quality is reduced to a minimum, and the seeds' vigorous germination and vitality are most effectively maintained, thereby ensuring the seeding value. The requirements for seed storage are good effect, simple and convenient operation, low cost and energy consumption, and a wide range of applications.

[0003] Safe seed storage is one of the key tasks from seed harvest to field sowing, which involves seed preservation and management. For seeds of any different plant, only by creating suitable storage conditions based on the storage characteristics of the seeds themselves can the germination and vitality of the seeds be maintained at the highest level during storage.

[0004] The storage of seeds is mainly affected by two aspects: external storage conditions and internal activities of seeds. The internal activities of seeds are mainly the respiration of seeds. The respiration of seeds is the process in which the living tissues in seeds (mainly active tissues such as embryos and aleurone cells) undergo a series of redox reactions with the participation of enzymes and oxygen to release carbon dioxide and water, and at the same time release energy, thereby providing the seeds with the energy needed for life activities. The respiration of seeds is mainly affected by moisture, temperature, oxygen content, the state of the seeds themselves, chemicals and other organisms. Appropriate respiration can promote seed ripening and drive away pests; however, excessive respiration will consume a large amount of stored dry matter in the seeds, increase the release of moisture and heat, thereby leading to the intensification of the activities of warehouse pests and microorganisms, further aggravating the ingestion and harm to seeds; in addition, anaerobic respiration can also produce toxic substances.

[0005] In order to isolate external intrusions and reduce the internal activity of seeds to extend the storage time, there are currently a variety of seed storage methods, including ordinary storage, sealed storage, vacuum storage, and low-temperature storage. Ordinary storage is a method of storing fully dried seeds in packaging materials in a warehouse. The temperature and humidity of the seeds will change due to the temperature and humidity in the warehouse. In actual production, in order to reduce storage costs, ordinary growers usually place seeds at room temperature or in a wet sand environment. These methods are easily affected by environmental interference, resulting in seed inactivation, being eaten by animals, or germination in advance.

[0006] Therefore, ordinary growers will dry the seeds to meet the moisture content standard under the sealing requirements, and then seal them with various containers or airtight materials (sealed storage), or seal the fully dried seeds in a container under near vacuum conditions for storage (vacuum storage). The purpose of these methods is to isolate the seeds from the outside world, inhibit the respiration of the seeds, and force the seeds to enter a dormant state, thereby achieving the purpose of extending the life of the seeds. However, ordinary storage, sealed storage and vacuum storage of seeds will consume the nutrients inside the seeds. After long-term storage, the vitality of the seeds will be greatly affected, making it difficult for the long-term stored seeds to germinate. Low-temperature storage is a method of storing seeds in a low-temperature environment. By reducing the temperature in the storage warehouse to below 15°C and the relative humidity to below 50%, the safety of the stored seeds is enhanced, thereby achieving the purpose of extending the life of the seeds, but low-temperature storage also faces the problem of affected seed vitality. At present, the long-term storage of plant seeds mainly uses ultra-low temperature refrigeration, but due to the influence of production facilities and high storage costs, production applications are limited, and it is mainly used for the long-term preservation of seed genetic resources.

[0007] There are differences in the storage environment conditions required by different seeds, and the storage method is directly related to the preservation time and sowing quality. Wax sealing is a new type of seed preservation storage technology, and its preservation principle is similar to the plastic sealing method. By wrapping a layer of film on the outside of the seeds, it can reduce the interference of the external environment, prevent internal moisture evaporation and external moisture intrusion, and inhibit seed respiration. This method can effectively extend the storage time of seeds and maintain the good quality of seeds. However, there are currently no exact regulations for the wax sealing method of seeds, and the wax sealing composition formula in the prior art is complicated.

[0008] Camellia oleifera Abel. is a general term for oil-rich species in the genus Camellia (Camellia L.) of the Theaceae family. It is one of the most important economic forest tree species in my country. Camellia oleifera seeds are composed of embryo, endosperm, seed shell and other parts. When the seeds mature, the embryo is not fully developed, so it has a shallow morphological dormancy characteristic. Gaozhou Camellia (C. gauchowensis Chang), also known as Vietnamese Camellia, is the southernmost Camellia species in my country. It has the characteristics of tall tree body, long life, large fruit, high single plant yield, strong disease resistance, high temperature resistance and high humidity resistance. However, Gaozhou Camellia oleifera is a kind of large-fruit Camellia oleifera, with large single fruit weight, thick pericarp, high seed yield and high oil content in seed kernel, which makes its storage points different from other varieties of Camellia oleifera. Summary of the invention

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a wax sealing composition for seed wax sealing storage and application thereof.

[0010] A first object of the present invention is to provide a wax seal composition for seed wax seal storage.

[0011] A second object of the present invention is to provide a wax sealing liquid for wax-sealed storage of seeds.

[0012] The third object of the present invention is to provide a seed storage method.

[0013] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0014] The invention claims a wax sealing composition for wax sealing storage of seeds, wherein the wax sealing composition is prepared by mixing 16-20 parts of carboxymethyl chitosan, 6-10 parts of polyvinyl alcohol, 6-14 parts of propylene glycol and 11-13 parts of glycerol by weight.

[0015] Preferably, the wax sealing composition is prepared by mixing 18 parts of carboxymethyl chitosan, 8 parts of polyvinyl alcohol, 10 parts of propylene glycol and 12 parts of glycerol, calculated by mass.

[0016] The use of the wax sealing composition in the preparation of a wax sealing liquid for wax-sealed storage of seeds should also be within the protection scope of the present invention.

[0017] More preferably, the wax sealing composition is used for wax sealing and storage of tea oil seeds.

[0018] Most preferably, the wax sealing composition is used for wax sealing storage of Gaozhou Camellia oleifera seeds.

[0019] A wax sealing liquid for wax-sealed storage of seeds, the wax sealing liquid being a mixture of the wax sealing composition and water which are fully mixed, wherein the ratio of the wax sealing composition to water is 1:25-75 by volume.

[0020] Preferably, the wax sealing liquid is a mixture of the wax sealing composition and water which are fully mixed, and the ratio of the wax sealing composition to water is 1:50 by volume.

[0021] More preferably, the wax sealing liquid is used for wax sealing and storage of tea oil seeds.

[0022] Most preferably, the wax sealing liquid is used for wax sealing and storage of Gaozhou Camellia oleifera seeds.

[0023] A seed storage method comprises the steps of wax-sealing the dried seeds with the wax-sealing liquid for storage.

[0024] Preferably, the seeds are camellia oleifera seeds.

[0025] More preferably, the seeds are Gaozhou camellia oleifera seeds.

[0026] Further more preferably, the seeds are Gaozhou camellia oleifera seeds, and the dry seeds are seeds with a moisture content of no more than 13%.

[0027] Specifically, the storage method is to use the wax sealing liquid to wax-seal the dry seeds for preservation, and the wax sealing method is: soak the dry seeds in the wax sealing liquid, fully soak them to ensure that the surface of the seeds is completely covered with the wax sealing layer, take out the seeds covered with the wax sealing layer, and disperse them to dry in the shade.

[0028] Preferably, the wax sealing method is to soak the dry seeds in the wax sealing liquid, fully soak them, and pick them up after 30 to 40 minutes. The amount of wax sealing liquid used is 1 to 3 L per 1 kg of seeds, ensuring that the surface of the seeds is completely covered with a wax sealing layer, take out the seeds covered with the wax sealing layer, and spread them out to dry in the shade.

[0029] More preferably, the wax sealing method is to soak the dry seeds in the wax sealing liquid, fully soak them, and pick them up after 30 minutes. The amount of wax sealing liquid used is 2L per 1kg of seeds to ensure that the surface of the seeds is completely covered with a wax sealing layer, take out the seeds covered with the wax sealing layer, and spread them out to dry in the shade.

[0030] Preferably, the dry seeds are sterilized dry seeds.

[0031] More preferably, the dry seeds are dry seeds that have been soaked and disinfected in a potassium permanganate solution.

[0032] Further more preferably, the dry seeds are dry seeds that have been soaked and disinfected in 5-10‰ potassium permanganate solution.

[0033] Most preferably, the dry seeds are dry seeds that have been disinfected by soaking in 5-10‰ potassium permanganate solution for 30-45 minutes.

[0034] More preferably, the dry seeds are dry seeds that have been disinfected by soaking in 5‰ potassium permanganate solution for 30 minutes.

[0035] Preferably, the storage temperature of the storage method is 2-25°C.

[0036] More preferably, the storage temperature of the storage method is 2 to 5°C.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] (1) The storage method of the present invention sterilizes the surface of the seed coat after the seeds are naturally dried, which can effectively kill bacteria and fungi carried on the seed coat, avoid the phenomenon of seed deterioration during storage, facilitate the normal respiration of the seeds, and prevent seedling diseases caused by pathogenic bacteria during seed germination.

[0039] (2) In the process of drying the Gaozhou tea seeds, the natural drying method is adopted to control the moisture content of the Gaozhou tea seeds to be lower than 13%, so that the embryo and the outer seed coat are separated, and the Gaozhou tea seeds are close to the minimum safety line for storage. After the Gaozhou tea seeds are completely dried, the physiological and biochemical effects inside are weakened, which can effectively avoid nutrient loss. At the same time, the interior of the Gaozhou tea seeds after drying is relatively dry, which is not conducive to the reproduction of microorganisms and can effectively inhibit the activity of microorganisms, thereby achieving the effect of maintaining the quality of the Gaozhou tea seeds and extending the storage time of the Gaozhou tea seeds.

[0040] (3) After soaking the seeds, the wax sealing composition and wax sealing liquid claimed in the present invention will form a dense protective film on the surface of the seed coat. The protective film has good permeability and water barrier properties, will not have a negative impact on the growth and development of the seeds, and can also prevent the loss and penetration of water inside the seeds, help inhibit the physiological and biochemical effects inside the seeds, further maintain a suitable moisture environment inside the seeds, prolong the storage time of the seeds and promote seed germination.

[0041] In addition, the protective film can block direct contact between moisture in the air and the seeds, and can better resist the harsh environment during storage, such as humidity changes and temperature fluctuations, thereby further improving the safety of seed storage.

[0042] Moreover, the protective film can effectively inhibit the infection and damage of exogenous bacteria to seeds, reduce the risk of seeds getting sick during storage, maintain seed vitality, and improve the quality of seedlings after germination.

[0043] The chitosan and polyvinyl alcohol in the protective agent work together to make the protective agent have good film-forming properties, and the formed film is not easily damaged, which increases the surface hardness and compression resistance of the seeds, reduces the physical damage of the external environment to the seeds, and increases their resistance during storage, transportation and sowing.

[0044] (4) The wax sealing composition and wax sealing liquid claimed in the present invention do not require heating during the preparation process. Therefore, the seed storage method claimed in the present invention does not require high-temperature heating when wax-sealing seeds, thereby avoiding the potential damage to seeds caused by high-temperature heating in the traditional wax-sealing process and better maintaining the vitality level of the seeds. Since the preparation process of the wax sealing composition and the wax sealing liquid and the process of wax-sealing seeds only require room temperature, there is no need to use special equipment or control the temperature, which simplifies the operation process and saves energy, complies with the principle of sustainable development, reduces energy consumption, and is more environmentally friendly.

[0045] (5) The present invention uses wax sealing as the main method for processing samples. Compared with the currently commonly used seed storage methods such as room temperature storage, low temperature storage and plastic sealing storage, the storage method claimed in the present invention has the advantages of improved germination rate and germination potential, long plumules, long radicles, large plumule diameters and radicle diameters. Compared with room temperature (no wax sealing) treatment, the shelf life can be extended by about one month. The wax-sealed Gaozhou tea seeds can be stored in a constant temperature or low temperature room with suitable temperature and humidity for 5 months or even longer.

[0046] Compared with the ultra-low temperature storage method, the storage method of the present invention does not require expensive equipment and a large amount of energy consumption, and is more feasible; it helps to reduce the use of energy resources and reduce the impact on the environment, which is in line with the country's new ideas for promoting sustainable agricultural development; the implementation process is simple and easy to operate, and it has high application value and application prospects in production activities. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 Schematic diagram of samples being bagged after collection;

[0048] Figure 2 Schematic diagram of samples in composting treatment to avoid light;

[0049] Figure 3 The samples are after being sun-dried and stripped;

[0050] Figure 4 This is a schematic diagram of the sample being disinfected;

[0051] Figure 5 This is a schematic diagram of the sample after the seeds are dried;

[0052] Figure 6 This is a wax seal stock solution diagram;

[0053] Figure 7 Schematic diagram of the sample during wax dipping treatment;

[0054] Figure 8 Schematic diagram of the sample after processing;

[0055] Fig. 9 For the sowing map;

[0056] Fig.10 This is a germination diagram.

[0057] Fig.11 This is a germination diagram for effect testing.

[0058] Fig.12 The figure is a comparison chart of germination rate and germination potential in different treatment groups.

[0059] Fig.13 Comparison of plumule and radicle lengths in different treatment groups.

[0060] Fig.14 Comparison of plumule and radicle diameters in different treatment groups. DETAILED DESCRIPTION

[0061] The present invention is further described in detail below in conjunction with the accompanying drawings and specific examples of the specification. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0062] Example 1

[0063] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0064] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits and put them into pre-prepared perforated storage bags (such as Figure 1 and transported back to base.

[0065] (2) Composting and ripening: The harvested fresh fruit is placed in a sealed oxygen-free environment and protected from light for 3 to 4 days (e.g. Figure 2 As shown), the fruits are piled up and fermented before ripening.

[0066] (3) Drying and de-fermenting: Spread the tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits gradually dry out the moisture and crack naturally (such as Figure 3 shown).

[0067] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0068] (5) Seed disinfection: Soak the collected Gaozhou oil-tea camellia seeds in 0.5% potassium permanganate solution for 30 min for disinfection (such as Figure 4 shown).

[0069] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place to dry in the sun, turning them over every day to prevent water accumulation and mildew (such as Figure 5 After drying for about 15 days, select large and full seeds, remove impurities and seal with wax for storage.

[0070] (7) Waxing: 2 L of wax sealing solution is required to treat 1 kg of Gaozhou oil-tea camellia seeds. The wax sealing solution is prepared in a ratio of stock solution to water = 1:50. The wax sealing stock solution is composed of 18 parts of carboxymethyl chitosan, 8 parts of polyvinyl alcohol, 10 parts of propylene glycol and 12 parts of glycerol by weight (the wax sealing stock solution is as follows: Figure 6 Soak the seeds completely in the wax sealing solution, stirring constantly to ensure that the surface of the seeds is completely covered with a thin layer of wax (as shown). Figure 7 After 30 min, remove from the water.

[0071] (8) Drying: Pick up the wax-soaked seeds and place them in a dry, dark and ventilated place to dry in the shade. After drying for 5 days, put them into a mesh perforated seed bag (such as Figure 8 Store at room temperature (24±1℃) in a ventilated, dry and dark place indoors.

[0072] Example 2

[0073] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0074] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits and put them into pre-prepared perforated storage bags (such as Figure 1 and transported back to base.

[0075] (2) Composting and ripening: The harvested fresh fruit is placed in a sealed oxygen-free environment and protected from light for 3 to 4 days (e.g. Figure 2 As shown), the fruits are piled up and fermented before ripening.

[0076] (3) Drying and de-fermenting: Spread the tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits gradually dry out the moisture and crack naturally (such as Figure 3 shown).

[0077] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0078] (5) Seed disinfection: Soak the collected Gaozhou oil-tea camellia seeds in 0.5% potassium permanganate solution for 30 min for disinfection (such as Figure 4 shown).

[0079] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place to dry in the sun, turning them over every day to prevent water accumulation and mildew (such as Figure 5After drying for about 15 days, select large and full seeds, remove impurities and seal with wax for storage.

[0080] (7) Waxing: 2 L of wax sealing solution is required to treat 1 kg of Gaozhou oil-tea camellia seeds. The wax sealing solution is prepared in a ratio of stock solution to water = 1:50. The wax sealing stock solution is composed of 20 parts by weight of carboxymethyl chitosan, 10 parts by weight of polyvinyl alcohol, 14 parts by weight of propylene glycol, and 13 parts by weight of glycerol (the wax sealing stock solution is as follows: Figure 6 Soak the seeds completely in the wax sealing solution, stirring constantly to ensure that the surface of the seeds is completely covered with a thin layer of wax (as shown). Figure 7 After 30 min, remove from the water.

[0081] (8) Drying: Pick up the wax-soaked seeds and place them in a dry, dark and ventilated place to dry in the shade. After drying for 5 days, put them into a mesh perforated seed bag (such as Figure 8 Store at room temperature (24±1℃) in a ventilated, dry and dark place indoors.

[0082] Example 3

[0083] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0084] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0085] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits and put them into pre-prepared perforated storage bags (such as Figure 1 and transported back to base.

[0086] (2) Composting and ripening: The harvested fresh fruit is placed in a sealed oxygen-free environment and protected from light for 3 to 4 days (e.g. Figure 2 As shown), the fruits are piled up and fermented before ripening.

[0087] (3) Drying and de-fermenting: Spread the tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits gradually dry out the moisture and crack naturally (such as Figure 3 shown).

[0088] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0089] (5) Seed disinfection: Soak the collected Gaozhou oil-tea camellia seeds in 0.5% potassium permanganate solution for 30 min for disinfection (such as Figure 4 shown).

[0090] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place to dry in the sun, turning them over every day to prevent water accumulation and mildew (such as Figure 5 After drying for about 15 days, select large and full seeds, remove impurities and seal with wax for storage.

[0091] (7) Waxing: 2 L of wax sealing solution is required for treating 1 kg of Gaozhou oil-tea camellia seeds. The wax sealing solution is prepared in a ratio of stock solution to water = 1:50. The wax sealing stock solution consists of 16 parts by weight of carboxymethyl chitosan, 6 parts by weight of polyvinyl alcohol, 6 parts by weight of propylene glycol, and 11 parts by weight of glycerol (the wax sealing stock solution is as follows: Figure 6 Soak the seeds completely in the wax sealing solution, stirring constantly to ensure that the entire surface of the seeds is covered with a thin layer of wax (as shown). Figure 7 After 30 min, remove from the water.

[0092] (8) Drying: Pick up the wax-soaked seeds and place them in a dry, dark and ventilated place to dry in the shade. After drying for 5 days, put them into a mesh perforated seed bag (such as Figure 8 Store at room temperature (24±1℃) in a ventilated, dry and dark place indoors.

[0093] Comparative Example 1

[0094] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0095] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits, put them into pre-prepared perforated storage bags, and transport them back to the base.

[0096] (2) Pile fermentation and ripening: The harvested fresh fruits are piled and fermented in a sealed oxygen-free environment, away from light for 3 to 4 days to allow the fruits to ripen after fermentation.

[0097] (3) Drying and removing the tea leaves: Spread the fermented tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits will gradually dry out the moisture and crack naturally.

[0098] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0099] (5) Seed disinfection: Soak the collected Gaozhou oil-tea tea seeds in 0.5% potassium permanganate solution for 30 minutes for disinfection.

[0100] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place for drying. Turn them over every day to prevent water accumulation and mildew. After drying for about 15 days, select large and plump seeds, remove impurities and seal them with wax for storage.

[0101] (7) Waxing: 2 L of wax sealing solution is required for each 1 kg of Gaozhou oil-tea camellia seeds. The wax sealing solution is prepared in a ratio of stock solution to water = 1:50. The wax sealing stock solution is composed of 15 parts of carboxymethyl chitosan, 5 parts of polyvinyl alcohol, 10 parts of glycerol, 5 parts of sorbitol and 5 parts of Honmirin by weight. The seeds are completely immersed in the wax sealing solution, stirring continuously to ensure that the entire surface of the seeds is covered with a thin layer of wax. After 30 minutes, pick them up.

[0102] (8) Drying: Pick up the wax-soaked seeds and place them in a dry, dark and ventilated place to dry in the shade. After drying for 5 days, put them into a mesh perforated seed bag and store them in a ventilated, dry and dark place indoors at room temperature (24±1°C).

[0103] Comparative Example 2

[0104] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0105] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits, put them into pre-prepared perforated storage bags, and transport them back to the base.

[0106] (2) Pile fermentation and ripening: The harvested fresh fruits are piled and fermented in a sealed oxygen-free environment, away from light for 3 to 4 days to allow the fruits to ripen after fermentation.

[0107] (3) Drying and removing the tea leaves: Spread the fermented tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits will gradually dry out the moisture and crack naturally.

[0108] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0109] (5) Seed disinfection: Soak the collected Gaozhou oil-tea tea seeds in 0.5% potassium permanganate solution for 30 minutes for disinfection.

[0110] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place for drying. Turn them over every day to prevent water accumulation and mildew. After drying for about 15 days, select large and plump seeds and remove impurities.

[0111] (7) Seed storage: The screened seeds were placed in a mesh perforated seed bag and stored at room temperature (24±1°C) in a ventilated, dry and dark place indoors.

[0112] Comparative Example 3

[0113] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0114] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits, put them into pre-prepared perforated storage bags, and transport them back to the base.

[0115] (2) Pile fermentation and ripening: The harvested fresh fruits are piled and fermented in a sealed oxygen-free environment, away from light for 3 to 4 days to allow the fruits to ripen after fermentation.

[0116] (3) Drying and removing the tea leaves: Spread the fermented tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits will gradually dry out the moisture and crack naturally.

[0117] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0118] (5) Seed disinfection: Soak the collected Gaozhou oil-tea tea seeds in 0.5% potassium permanganate solution for 30 minutes for disinfection.

[0119] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place for drying. Turn them over every day to prevent water accumulation and mildew. After drying for about 15 days, select large and plump seeds and remove impurities.

[0120] (7) Seed storage: Place the screened seeds into mesh perforated seed bags and store them in a seed cabinet at 2°C to 5°C.

[0121] Comparative Example 4

[0122] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits, put them into pre-prepared perforated storage bags, and transport them back to the base.

[0123] (2) Pile fermentation and ripening: The harvested fresh fruits are piled and fermented in a sealed oxygen-free environment, away from light for 3 to 4 days to allow the fruits to ripen after fermentation.

[0124] (3) Drying and removing the tea leaves: Spread the fermented tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits will gradually dry out the moisture and crack naturally.

[0125] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0126] (5) Seed disinfection: Soak the collected Gaozhou oil-tea tea seeds in 0.5% potassium permanganate solution for 30 minutes for disinfection.

[0127] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place for drying. Turn them over every day to prevent water accumulation and mildew. After drying for about 15 days, select large and plump seeds and remove impurities.

[0128] (7) Plastic sealing: Put the dried Gaozhou oil-tea tea seeds into a transparent vacuum bag and seal it with a vacuum sealer.

[0129] (8) Seed storage: Store the plastic-sealed seeds in a ventilated, dry, and dark place indoors at room temperature (24±1℃).

[0130] Comparative Example 5

[0131] A Gaozhou oil-tea camellia seed storage method comprises the following steps:

[0132] (1) Fruit collection: Weed and clean the forest in advance, select high-yield fruit forests, collect ripe tea oil fruits, put them into pre-prepared perforated storage bags, and transport them back to the base.

[0133] (2) Pile fermentation and ripening: The harvested fresh fruits are piled and fermented in a sealed oxygen-free environment, away from light for 3 to 4 days to allow the fruits to ripen after fermentation.

[0134] (3) Drying and removing the tea leaves: Spread the fermented tea fruits evenly and without overlapping in a cool, dry and ventilated place to dry them. After natural drying, the fresh fruits will gradually dry out the moisture and crack naturally.

[0135] (4) Cattail seed separation: After drying in the sun, some of the Gaozhou tea seeds naturally separate from the fruit skin. The unopened tea fruits need to be opened manually. The Gaozhou tea seeds in the fresh fruits are collected and the seed quality is checked according to the requirements of GB / T 2772. At the same time, the seed moisture content is measured and seeds with unqualified quality and moisture content greater than 13% are screened out.

[0136] (5) Seed disinfection: Soak the collected Gaozhou oil-tea tea seeds in 0.5% potassium permanganate solution for 30 minutes for disinfection.

[0137] (6) Seed drying: Place the disinfected Gaozhou oil-tea camellia seeds in a dry, dark and ventilated place for drying. Turn them over every day to prevent water accumulation and mildew. After drying for about 15 days, select large and plump seeds, remove impurities and seal them with wax for storage.

[0138] (7) Waxing: 2 L of wax sealing solution is required for each 1 kg of Gaozhou oil-tea camellia seeds. The wax sealing solution is prepared in a ratio of stock solution to water = 1:50. The wax sealing stock solution consists of 8 parts of carboxymethyl chitosan, 5 parts of polyvinyl alcohol, 5 parts of propylene glycol and 10 parts of glycerol by weight. The seeds are completely immersed in the wax sealing solution, stirring continuously to ensure that the entire surface of the seeds is covered with a thin layer of wax. After 30 minutes, pick them up.

[0139] (8) Drying: Pick up the wax-soaked seeds and place them in a dry, dark and ventilated place to dry in the shade. After drying for 5 days, put them into a mesh perforated seed bag and store them in a ventilated, dry and dark place indoors at room temperature (24±1°C).

[0140] Example 4 Effect test of different storage methods

[0141] 1. Inspection method

[0142] (1) Three bags of fresh seeds that were not dried after threshing were randomly selected for the first germination test.

[0143] (2) The Gaozhou tea oil seeds that passed the inspection in Example 1 were stored using the Gaozhou tea oil seed storage method of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5. After 60 days of storage, a seed germination test was performed every 30 days.

[0144] The test steps are as follows: from the Gaozhou tea seeds stored by the different Gaozhou tea seed storage methods, 3 bags of seeds are randomly selected for germination in each treatment group (as 3 repetitions). Before germination, the seeds are soaked in warm water at 60°C for 12 hours to accelerate germination and break the dormancy of the Gaozhou tea seeds. After germination, the Gaozhou tea seeds are evenly sown in a transparent container covered with 10 cm thick wet sand, so that the Gaozhou tea seeds are close to the wall of the transparent container for easy observation of germination. 3 to 5 layers of sterilized gauze are laid on the bottom of the transparent container, and the germination test is carried out in an artificial climate greenhouse, and the temperature in the artificial climate greenhouse is controlled to be (25±1)°C and the humidity is 80%. Observe the seed germination on time every day, replenish water as needed, keep the sand bed moist, and remove mildewed and infected seeds in time. Supplement light at night every day, and the germination is considered to be terminated when the Gaozhou tea seeds no longer germinate for 5 consecutive days.

[0145] The seeds stored by the Gaozhou camellia oil seed storage method of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5 were inactivated for two consecutive months, and the seed germination test was stopped. The germination rate and germination potential of the Gaozhou camellia oil seeds stored by the Gaozhou camellia oil seed storage method of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5, the length of the embryo and the length of the radicle of the Gaozhou camellia oil seeds, and the diameter of the embryo and the diameter of the radicle of the Gaozhou camellia oil seeds were counted.

[0146] The measurement method of radicle and plumule was as follows: after germination was terminated, 10 seedlings were randomly selected from each treatment group (all were measured if the number was insufficient) and the radicle length and diameter, plumule length and diameter of the germinated seeds were measured using a ruler (0.1 cm) and a vernier caliper (0.01 mm).

[0147] The measurement method of germination rate and germination potential is as follows: after sowing, observe every day, observe the rooting and germination through a transparent container, and record the number of seeds that germinate every day. The germination of seeds is based on the appearance of white as the standard. After germination ends, the germination rate and germination potential are calculated as follows:

[0148]

[0149] 2. Test results

[0150] The technical effects of Examples 1 to 3 are all better than those of Comparative Examples 1 to 5. Since Example 1 has the best technical effect, Example 1 is selected as a representative example of Examples 1 to 3 for drawing.

[0151] Depend on Fig.12 It can be seen that using Example 1 ( Fig.12 The seeds stored by the Gaozhou Camellia oleifera seed storage method (shown in group S1) are the same as those stored by the comparative example 1 ( Fig.12 D1 group), Comparative Example 2 ( Fig.12 D2 group), Comparative Example 3 ( Fig.12 D3 group), Comparative Example 4 ( Fig.12 D4 group) and Comparative Example 5 ( Fig.12 Compared with the seeds stored by the Gaozhou oil-tea camellia seed storage method of Example 1 (shown in Group D5), the seeds still had a germination rate of nearly 20% after being stored for 5 months. The germination potential of the seeds reflects the vitality of the seeds, and the level of germination potential can be used to evaluate the quality and storage status of the seeds. Compared with conventional room temperature storage (Comparative Example 2), low temperature refrigeration (Comparative Example 3) and plastic-sealed storage (Comparative Example 4), the seeds stored by the Gaozhou oil-tea camellia seed storage method of Example 1 had a higher survival rate and a higher germination potential, which means that the seeds stored by the Gaozhou oil-tea camellia seed storage method of Example 1 also had higher vitality.

[0152] In the wax sealing solution used in Comparative Example 1, sorbitol and Ben Mirin are both commonly used seed protectants, among which sorbitol has good water retention performance, can help maintain the moisture content of seeds, prevent seeds from dehydration, and slow down the consumption and degradation process of nutrients inside seeds; Ben Mirin contains a variety of beneficial ingredients, such as amino acids, vitamins and organic acids, which have the effect of promoting seed germination and growth, and can increase the ability of seeds to resist corruption and pathogens, thereby improving the quality and survival rate of seeds. However, the germination rate and germination potential of Comparative Example 1, which uses sorbitol and Ben Mirin and wax sealing solution made of carboxymethyl chitosan, polyvinyl alcohol and glycerol to wrap camellia seeds for room temperature storage, are not as good as those of the storage methods of Implementations 1 to 3. This is because the specific wax sealing solution components and ratios will affect the storage effect of seeds, and the interaction, combination and ratio of multiple chemical substances will lead to differences in storage effects.

[0153] In Comparative Example 5, in which wax sealing solutions with different main component ratios were used to wrap camellia seeds for storage at room temperature, the germination rate and germination potential were not as good as those of the storage methods of Examples 1 to 3. This shows that the Gaozhou camellia seed storage method of Examples 1 to 3 can protect the seeds from water loss and interference from microorganisms in the environment, maintain a suitable gas exchange balance during storage, significantly improve the survival rate and germination rate of the seeds during storage, and the preservation state and quality of the seeds are better, which is more conducive to rapid germination and growth into seedlings.

[0154] Depend on Fig.13 It can be seen that using Example 1 ( Fig.13 The seeds stored by the Gaozhou Camellia oleifera seed storage method (shown in group S1) are the same as those stored by the comparative example 1 ( Fig.13 D1 group), Comparative Example 2 ( Fig.13 D2 group), Comparative Example 3 ( Fig.13 D3 group), Comparative Example 4 ( Fig.13 D4 group) and Comparative Example 5 ( Fig.13 Compared with the seeds stored by the Gaozhou Camellia oleifera seed storage method (shown in group D5), the length of the embryo and the radicle was the longest.

[0155] Depend on Fig.14 It can be seen that using Example 1 ( Fig.14 The seeds stored by the Gaozhou Camellia oleifera seed storage method (shown in group S1) are the same as those stored by the comparative example 1 ( Fig.14 D1 group), Comparative Example 2 ( Fig.14 D2 group), Comparative Example 3 ( Fig.14 D3 group), Comparative Example 4 ( Fig.14 D4 group) and Comparative Example 5 ( Fig.14 Compared with the seeds stored by the Gaozhou Camellia oleifera seed storage method (shown in group D5), the diameters of the plumule and radicle were the longest.

[0156] The plumule is the original plant bud in the seed. The good development of the plumule means that the seed has a higher germination ability and growth potential; the radicle is the original root system in the seed. The developed radicle is conducive to the seed to obtain sufficient water and nutrients at the beginning of the growth stage. The health and development status of the plumule and radicle are crucial to the germination and growth of the seed. Therefore, the seeds stored using the Gaozhou oil-tea camellia seed storage method of Examples 1 to 3 are well maintained in seed vitality, which can effectively delay seed aging and degradation and increase the survival rate of oil-tea camellia seedlings after germination. Moreover, the radicle and plumule have a longer length and diameter, indicating that they have accumulated sufficient nutrients and energy reserves during storage. Therefore, the seeds stored using the Gaozhou oil-tea camellia seed storage method of Examples 1 to 3 preserve more nutrients, which is conducive to the rapid growth and healthy development of oil-tea camellia seedlings after germination.

[0157] This shows that the Gaozhou tea oil seed storage method of Examples 1 to 3 can keep the embryo and radicle of the seeds in a good state, which can bring significant benefits to the preservation, germination and subsequent growth of the Gaozhou tea oil seedlings, so that the Gaozhou tea oil seeds stored for a long time can retain more seed vitality, survival rate and development potential.

[0158] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A wax seal composition for seed wax seal storage, characterized in that: The wax sealing composition is prepared by mixing 16 to 20 parts of carboxymethyl chitosan, 6 to 10 parts of polyvinyl alcohol, 6 to 14 parts of propylene glycol and 11 to 13 parts of glycerol, calculated by mass.

2. Use of the wax sealing composition according to claim 1 in preparing a wax sealing liquid for wax-sealed storage of seeds.

3. A wax sealing liquid for wax-sealed storage of seeds, characterized in that: The wax sealing liquid is a mixture of the wax sealing composition according to claim 1 and water which are fully mixed. Calculated by volume, the wax sealing composition according to claim 1: water = 1:25-75.

4. A seed storage method, characterized in that: The storage method comprises wax-sealing the dried seeds using the wax-sealing liquid according to claim 3 for storage.

5. The method according to claim 4, characterized in that The seeds are camellia oleifera seeds.

6. The method according to claim 5, characterized in that The seeds are Gaozhou camellia oleifera seeds.

7. The method according to claim 6, characterized in that The seeds are Gaozhou tea oil seeds, and the dry seeds are seeds with a water content of no more than 13%.

8. The method according to claim 4, characterized in that The specific method of wax sealing is: soaking the dry seeds in the wax sealing liquid of claim 3, fully soaking to ensure that the surface of the seeds is completely covered with the wax sealing layer, taking out the seeds covered with the wax sealing layer, and drying them in the shade.

9. The method according to claim 8, characterized in that The dry seeds are sterilized dry seeds.

10. The method according to claim 4, characterized in that The storage temperature is 2-25°C.

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