A preparation method of Huanghe oral liquid for replenishing qi and nourishing blood

Abstract

A preparation method of Huang He oral liquid for invigorating qi and nourishing blood belongs to the technical field of medicines and health products. The method comprises preparing a decoction extract of traditional Chinese medicine, preparing a fermentation hydrolyzate using traditional Chinese medicine filter residue, barley flour, barley bran powder and roasted highland barley bran powder, and adding bovine liver protein peptide, sheep liver phosphorylated peptide, fruit flavor, sweetener and sodium alginate to prepare Huang He oral liquid. The method of the present invention adds fermentation hydrolyzate of traditional Chinese medicine residue and barley flour, barley bran powder and roasted highland barley bran powder to the decoction extract of traditional Chinese medicine, and adds bovine liver protein peptide and sheep liver phosphorylated peptide components for use in combination. The peptide components and the fermentation hydrolyzate of traditional Chinese medicine can play a therapeutic effect in the short term, enhance the effect of invigorating blood and invigorating qi, resist fatigue and weakness, and quickly relieve symptoms such as mental fatigue and insomnia; the decoction extract of traditional Chinese medicine plays a better therapeutic effect in the medium and long term, and the use of each other can greatly improve the efficacy and shorten the course of treatment, and the conditioning effect is good, and there is no dependence and recurrence.

Description

Technical Field

[0001] The invention belongs to the technical field of medicines and health products, and particularly relates to a method for preparing Huanghe oral liquid for invigorating qi and nourishing blood. Background Art

[0002] With the accelerated pace of modern people's lives, bad habits such as long hours in front of computer or mobile phone screens, frequent staying up late, and eating unhealthy foods can lead to sub-healthy liver and kidney symptoms, persistent fatigue, deficiency of qi and blood, dizziness, palpitations and insomnia, etc. Over time, mental and life problems such as bad temper, memory loss, lack of concentration, and lack of interest in everything will occur, so physical conditioning is needed, including diet conditioning, exercise regulation, and drug intervention.

[0003] Among them, Huang He oral liquid is used to nourish qi and blood, tonify liver and kidney, treat dizziness, palpitations and insomnia caused by qi and blood deficiency and liver and kidney deficiency, and can regulate sub-health symptoms. Huang He oral liquid is a light reddish brown to dark reddish brown liquid preparation made mainly from Chinese medicinal ingredients such as Gynostemma pentaphyllum, Polygonatum sibiricum, Ganoderma lucidum, Polygonum multiflorum, Panax notoginseng, Gastrodia elata, Eucommia ulmoides, Polygonatum odoratum, Cannabis sativa seed and Licorice, and has a bitter taste.

[0004] At present, Huang He oral liquid contains all Chinese herbal conditioning ingredients, which have the disadvantages of slow onset of effect, long course of treatment, and poor palatability, especially for those with sensitive taste buds, who have poor medication compliance. Summary of the invention

[0005] In view of the problems of slow onset of effect, long course of treatment, and poor palatability of the existing Huang He oral liquid, the present invention provides a preparation method of Huang He oral liquid for invigorating qi and nourishing blood, adding Chinese medicine residues and fermented enzymatic hydrolysate of barley flour, barley bran powder and roasted highland barley bran powder to the Chinese medicine decoction extract, and adding bovine liver protein peptides and sheep liver phosphorylated peptide components for use in combination, the peptide components and the Chinese medicine fermentation enzymatic hydrolysate can play a therapeutic effect in the short term, enhance the effect of invigorating blood and invigorating qi, resist fatigue and weakness, and quickly relieve symptoms such as mental fatigue and insomnia; the Chinese medicine decoction extract plays a better therapeutic effect in the medium and long term, and the use of each other can greatly improve the efficacy and shorten the course of treatment, with good conditioning effect and no dependence and recurrence. The specific technical scheme is as follows:

[0006] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0007] S1: Weigh 1 to 5 parts by weight of Gynostemma pentaphyllum, Polygonatum sibiricum, Ganoderma lucidum, Polygonatum multiflorum, Panax notoginseng, Gastrodia elata, Eucommia ulmoides, Polygonatum odoratum, Fructus hemp and Licorice respectively, grind and mix to obtain medicinal material powder A;

[0008] S2: decoct the medicinal material powder A with water, filter to obtain a residue B and a filtrate C, and concentrate the filtrate to obtain a medicinal solution D;

[0009] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1: (0.3-0.5): (0.05-0.2): (0.1-0.3), the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, salt water and lactic acid bacteria are added to the mixture E, and anaerobic fermentation is performed to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis, and a filtrate is obtained by filtering, and the filtrate is concentrated to obtain a liquid medicine G;

[0010] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = (1-5): (0.5-2): (0.1-0.5): (0.1-0.5): (0.01-0.1): (0.01-0.1): (0.05-0.1): (0.5-1), first add water to boil the fruit flavor, sweetener and sodium alginate to dissolve, reduce the temperature to 40-42°C, then add liquid D and liquid G and mix evenly, then add bovine liver protein peptide and sheep liver phosphorylated peptide, mix to obtain Huang He oral liquid.

[0011] In S1 of the above method, the particle size of the crushed material is less than 1 mm.

[0012] In S2 of the above method, the amount of water added for decoction is 8 to 15 times the mass of the medicinal material powder A; the decoction temperature is boiling water, maintained for 20 to 40 minutes, and the concentrated filtrate is an extract with a relative density of 1.5 to 2.0.

[0013] In S3 of the above method, the baking temperature of the highland barley bran powder is 150-200° C., and the baking time is 30-60 min.

[0014] In S3 of the above method, the concentration of the brine is 8 to 10 g / 100 mL, and the amount of brine added is 6 to 10 times the mass of the mixture E.

[0015] In S3 of the above method, the anaerobic fermentation temperature is 36-37° C., and the anaerobic fermentation time is 48-60 hours.

[0016] In S3 of the above method, the amount of amylase added is 0.05-0.15% of the mass of the fermentation liquid F, and the amylase activity unit is 2000-2500 U / g.

[0017] In S3 of the above method, the amount of xylanase added is 0.05-0.15% of the mass of the fermentation liquid F, and the activity unit of the xylanase is 40000-50000 U / g.

[0018] In S3 of the above method, the enzymatic hydrolysis time is 3 to 5 hours.

[0019] In S3 of the above method, the concentrated filtrate is an extract having a relative density of 1.5 to 2.0.

[0020] Compared with the prior art, the preparation method of the Qi-invigorating and blood-nourishing Huanghe oral liquid of the present invention has the following beneficial effects:

[0021] 1. The present invention improves the formula and preparation method of conventional Huang He oral liquid, uses the decoction of Chinese medicine ingredients to extract the filter residue, ferments with barley flour, barley bran powder, and baked highland barley bran powder, and then performs enzymolysis to obtain a milder and easier to absorb active ingredient, wherein barley flour and barley bran powder are fermented and enzymolyzed with Chinese medicine ingredients to obtain Jianshenqu ingredients, which have the effects of regulating qi and removing dampness, strengthening the spleen and stomach, and promoting rapid absorption of drugs. And the preparation of the decoction of Chinese medicine ingredients to extract the filter residue can maximize the utilization of Chinese medicine by-products, waste utilization, and cost saving. Among them, highland barley bran can increase the generated content of Jianshenqu, and after baking, highland barley bran can improve the fermentation and enzymolysis efficiency and shorten the process time; the product generated after enzymolysis is more easily absorbed by the human body, and the efficacy is improved.

[0022] 2. The Huang He oral liquid of the present invention also adds a specific proportion of bovine liver protein peptides, which can be quickly absorbed by the human body after taking it, promote the efficacy, and shorten the course of treatment. The bovine liver protein peptides can promote the excretion of toxins accumulated in the human body, promote the synthesis of hemoglobin, play a good role in nourishing blood and qi, and also have the effects of maintaining skin health, protecting eyes, relieving dizziness, and preventing vision loss; it can maintain the integrity of liver cells, improve the disease resistance of cells, prevent toxins from penetrating and destroying liver cell membranes, and prevent liver cell degeneration or necrosis; promote protein synthesis and liver cell regeneration, repair damaged liver cells, restore the normal function of the liver, improve immunity, and relieve symptoms such as mental fatigue and insomnia.

[0023] 3. The Huang He oral liquid of the present invention also adds a specific proportion of sheep liver phosphorylated peptides, which can be quickly absorbed by the human body after taking, promote the therapeutic effect, and shorten the course of treatment. Phosphorylated peptides play an important role in cellular energy metabolism. Sheep liver phosphorylated peptides can promote the regulation of muscle contraction and decontraction, have anti-fatigue and weakness effects, improve physical vitality, and have a good ability to resist oxygen free radicals.

[0024] Fourth, the Huang He oral liquid of the present invention also aims at maintaining good suspension of bovine liver protein peptides and sheep liver phosphorylated peptides with other ingredients, and also adds a specific proportion of sodium alginate to improve the mixing and suspension effects.

[0025] 5. The Huang He oral liquid of the present invention is also added with fruit flavoring agents and sweeteners to improve palatability and medication compliance.

[0026] 6. The preparation method of the present invention finally adds the bovine liver protein peptide and the sheep liver phosphorylated peptide at 40-42° C., which can maximize the effectiveness of the peptide components and is not prone to structural destruction due to mixing, or deterioration due to excessively high temperature, which affects the efficacy.

[0027] In summary, the oral liquid of the present invention contains various Chinese medicine decoction extracts, fermentation hydrolysates, bovine liver protein peptides and sheep liver phosphorylated peptides. The peptide components and the Chinese medicine fermentation hydrolysates can play a therapeutic effect in the short term, enhance the effect of nourishing blood and replenishing qi, resist fatigue and weakness, and quickly relieve symptoms such as mental fatigue and insomnia; the Chinese medicine extract components play a better therapeutic effect in the medium and long term, and the coordinated use of each other can greatly improve the efficacy and shorten the course of treatment, with good conditioning effect and no dependence and recurrence. Within the 1st to 10th day of taking the medicine, the symptoms of dizziness and vertigo were relieved by 45%, the symptoms of palpitations were relieved by 50%, and the symptoms of insomnia were relieved by 51%; within the 11th to 20th day of taking the medicine, the symptoms of dizziness and vertigo were relieved by 83%, the symptoms of palpitations were relieved by 86%, and the symptoms of insomnia were relieved by 91%; within the 21st to 30th day of taking the medicine, the symptoms of dizziness and vertigo were relieved by 100%, the symptoms of palpitations were relieved by 100%, and the symptoms of insomnia were relieved by 98%; there was no recurrence after stopping taking the medicine for the 60th day, indicating that the oral liquid of this embodiment has a quick and significant effect in treatment, no dependence on long-term use, and has good economic and practical value. DETAILED DESCRIPTION

[0028] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0029] Example 1

[0030] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0031] S1: Weigh parts by weight respectively, 3 parts of Gynostemma pentaphyllum, 2 parts of Polygonatum sibiricum, 1 part of Ganoderma lucidum, 2 parts of Polygonatum multiflorum, 4 parts of Panax notoginseng, 2 parts of Gastrodia elata, 1 part of Eucommia ulmoides, 1 part of Polygonatum odoratum, 1 part of Cannabis sativa seed and 1 part of Licorice, grind them to a particle size of less than 1 mm, and mix them to obtain medicinal material powder A.

[0032] S2: Decoct the medicinal powder A with water in an amount of 10 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 30 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 2.0 to obtain medicinal solution D.

[0033] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.4:0.1:0.2, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 8 times the mass of brine with a concentration of 9 g / 100 mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 37°C for 55 hours to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 3 hours, and the filtrate is filtered to obtain a filtrate, and the filtrate is concentrated to a relative density of 2.0 to obtain an extract to obtain a medicinal solution G.

[0034] The baking temperature of the baked highland barley bran powder is 150° C., and the baking time is 60 min.

[0035] The amount of amylase added is 0.05% of the mass of the fermentation liquid F, and the amylase activity unit is 2000 U / g. The amount of xylanase added is 0.05% of the mass of the fermentation liquid F, and the xylanase activity unit is 40000 U / g.

[0036] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 3:1:0.3:0.2:0.05:0.05:0.08:0.8. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After cooling to 40°C, add liquid D and liquid G and mix well. Then add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0037] Preparation Comparative Example 1: Step S3 was omitted, and liquid G was not added in step S4; other parameters were the same as those in this embodiment.

[0038] Preparation Comparative Example 2: In step S3, barley flour, barley bran powder and roasted highland barley bran powder are not added, and the filter residue B is directly fermented and enzymolyzed; other parameters are the same as those in this embodiment.

[0039] Preparation Comparative Example 3: In step S4, no bovine liver protein peptide and sheep liver phosphorylated peptide were added; other parameters were the same as those in this embodiment.

[0040] Preparation Comparative Example 4: Step S3 was omitted, and in Step S4, no drug solution G, bovine liver protein peptide and sheep liver phosphorylated peptide were added.

[0041] 1. Safety test:

[0042] (1) Acute toxicity test:

[0043] The oral liquids of this embodiment and comparative examples 1-4 were respectively administered to the test mice by gavage at twice the normal dose, and the drugs were continuously administered 3 times within 24 hours, each time with an interval of 4 hours, and the cumulative total amount of the drug reached 30 mL. Within 7 days after administration, the mice had normal activities, eating, and excretion, bright fur, and good health. The test mice were killed on the 8th day, and autopsy, organ coefficients, and histological examinations were performed, and the results were normal, indicating that the oral liquids of this embodiment and comparative examples 1-4 had no acute toxic reactions.

[0044] (2) Long-term toxicity test:

[0045] The oral liquids of this embodiment and comparative examples 1 to 4 were continuously administered to the test mice by oral gavage at a dose twice the normal dose for 3 months, and the external physical symptoms, behavioral activities, secretions, fecal characteristics, etc. were continuously observed. After 3 months, the test mice were killed for autopsy, organ coefficients and histological examinations, and the results showed no abnormalities, indicating that the oral liquids of this embodiment and comparative examples 1 to 4 had no long-term toxic reactions, and the composition was safe and non-toxic.

[0046] The selection of raw materials is within the range of types allowed for drug addition.

[0047] 2. Clinical Trials:

[0048] 100 subjects with qi and blood deficiency and liver and kidney sub-health symptoms were selected and randomly divided into 5 groups (20 subjects in each group), and the subjects took the oral liquids of this embodiment and comparative examples 1 to 4, respectively. The age, course of disease, etc. of the five groups of subjects had no significant significance (p>0.05), and were comparable.

[0049] Take once a day in the morning and evening, 5 ml each time after dilution, observe and statistically evaluate the results of the subjects as follows:

[0050]

[0051]

[0052] The above results show that the oral liquid of this embodiment, within the 1st to 10th day of taking: dizziness and vertigo symptoms are alleviated by 45%, palpitation symptoms are alleviated by 50%, and insomnia symptoms are alleviated by 51%; within the 11th to 20th day of taking: dizziness and vertigo symptoms are alleviated by 83%, palpitation symptoms are alleviated by 86%, and insomnia symptoms are alleviated by 91%; within the 21st to 30th day of taking: dizziness and vertigo symptoms are alleviated by 100%, palpitation symptoms are alleviated by 100%, and insomnia symptoms are alleviated by 98%; there is no recurrence after stopping taking the 60th day, indicating that the oral liquid of this embodiment has a quick effect, a significant effect, and no long-term use dependence. The oral liquids of Comparative Examples 1 and 2 are relatively slow to take effect, the effect is relatively poor, and there is long-term use dependence. The oral liquids of Comparative Examples 3 and 4 are very slow to take effect, have a long course of treatment, and the effect is relatively the worst, and there are a large number of recurrence symptoms when they are discontinued.

[0053] Example 2

[0054] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0055] S1: Weigh parts by weight respectively, 2.5 parts of Gynostemma pentaphyllum, 2 parts of Polygonatum sibiricum, 1.5 parts of Ganoderma lucidum, 2 parts of Polygonatum multiflorum, 3 parts of Panax notoginseng, 1 part of Gastrodia elata, 1.5 parts of Eucommia ulmoides, 2 parts of Polygonatum odoratum, 1 part of Cannabis sativa seed and 1 part of Licorice, grind them to a particle size of less than 1 mm, and mix them to obtain medicinal material powder A.

[0056] S2: Decoct the medicinal powder A with water in an amount of 8 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 35 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 1.8 to obtain medicinal solution D.

[0057] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.3:0.05:0.1, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 10 times the mass of brine with a concentration of 10g / 100mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 37°C for 60h to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 5h, and the filtrate is filtered to obtain the filtrate, and the filtrate is concentrated to a relative density of 1.8 extract to obtain the medicinal solution G.

[0058] The baking temperature of the baked highland barley bran powder is 200° C., and the baking time is 30 minutes.

[0059] The amount of amylase added is 0.15% of the mass of the fermentation liquid F, and the amylase activity unit is 2500 U / g. The amount of xylanase added is 0.15% of the mass of the fermentation liquid F, and the xylanase activity unit is 50000 U / g.

[0060] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 1:2:0.1:0.5:0.01:0.1:0.05:1. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After cooling to 42°C, add liquid D and liquid G and mix well. Then, add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0061] Example 3

[0062] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0063] S1: Weigh parts by weight respectively, 5 parts of Gynostemma pentaphyllum, 4 parts of Polygonatum sibiricum, 2 parts of Ganoderma lucidum, 5 parts of Polygonatum multiflorum, 3 parts of Panax notoginseng, 2.5 parts of Gastrodia elata, 1.5 parts of Eucommia ulmoides, 1.5 parts of Polygonatum odoratum, 1.5 parts of Cannabis sativa seed and 1.5 parts of Licorice, grind them to a particle size of less than 1 mm, and mix them to obtain medicinal material powder A.

[0064] S2: Decoct the medicinal powder A with water in an amount of 9 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 25 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 1.6 to obtain medicinal solution D.

[0065] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.5:0.2:0.3, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 6 times the mass of brine with a concentration of 9 g / 100 mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 37°C for 50 hours to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 4 hours, and the filtrate is filtered to obtain a filtrate, and the filtrate is concentrated to a relative density of 1.6 extract to obtain a medicinal solution G.

[0066] The baking temperature of the baked highland barley bran powder is 180° C., and the baking time is 50 min.

[0067] The amount of amylase added is 0.05% of the mass of the fermentation liquid F, and the amylase activity unit is 2500 U / g. The amount of xylanase added is 0.05% of the mass of the fermentation liquid F, and the xylanase activity unit is 50000 U / g.

[0068] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 5: 0.5: 0.5: 0.1: 0.1: 0.01: 0.1: 0.5. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After cooling to 41°C, add liquid D and liquid G and mix well. Then, add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0069] Example 4

[0070] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0071] S1: Weigh parts by weight respectively, 4 parts of Gynostemma pentaphyllum, 3 parts of Polygonatum sibiricum, 2 parts of Ganoderma lucidum, 3 parts of Polygonatum multiflorum, 5 parts of Panax notoginseng, 4 parts of Gastrodia elata, 4 parts of Eucommia ulmoides, 4 parts of Polygonatum odoratum, 3 parts of Fructus hemp and 3 parts of Licorice, grind them to a particle size of less than 1 mm, and mix them to obtain medicinal material powder A.

[0072] S2: Decoct the medicinal powder A with water in an amount of 12 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 40 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 2.0 to obtain medicinal solution D.

[0073] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.3:0.2:0.1, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 7 times the mass of brine with a concentration of 8g / 100mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 36°C for 60h to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 5h, and the filtrate is filtered to obtain the filtrate, and the filtrate is concentrated to a relative density of 2.0 to obtain an extract to obtain a medicinal solution G.

[0074] The baking temperature of the baked highland barley bran powder is 160° C., and the baking time is 50 min.

[0075] The amount of amylase added is 0.15% of the mass of the fermentation liquid F, and the amylase activity unit is 2000 U / g. The amount of xylanase added is 0.15% of the mass of the fermentation liquid F, and the xylanase activity unit is 40000 U / g.

[0076] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 1:0.5:0.1:0.1:0.01:0.01:0.05:0.5. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After the temperature drops to 40.5°C, add liquid D and liquid G and mix evenly. Then, add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0077] Example 5

[0078] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0079] S1: Weigh parts by weight respectively, 1 part of Gynostemma pentaphyllum, 1 part of Polygonatum sibiricum, 2 parts of Ganoderma lucidum, 1 part of Polygonatum multiflorum, 1 part of Panax notoginseng, 2.5 parts of Gastrodia elata, 5 parts of Eucommia ulmoides, 3 parts of Polygonatum odoratum, 3 parts of Cannabis sativa seed and 3.5 parts of Licorice, grind them to a particle size of less than 1 mm, and mix them to obtain medicinal material powder A.

[0080] S2: Decoct the medicinal powder A with water in an amount of 14 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 20 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 1.8 to obtain medicinal solution D.

[0081] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.5:0.05:0.3, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 6.5 times the mass of brine with a concentration of 8 g / 100 mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 36.5°C for 48 hours to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 4.5 hours, and the filtrate is filtered to obtain a filtrate, and the filtrate is concentrated to a relative density of 1.8 extract to obtain a medicinal solution G.

[0082] The baking temperature of the baked highland barley bran powder is 200° C., and the baking time is 40 minutes.

[0083] The amount of amylase added is 0.1% of the mass of the fermentation liquid F, and the amylase activity unit is 2200 U / g. The amount of xylanase added is 0.1% of the mass of the fermentation liquid F, and the xylanase activity unit is 45000 U / g.

[0084] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 5:2:0.5:0.5:0.1:0.1:0.1:1. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After the temperature drops to 41.5°C, add liquid D and liquid G and mix evenly. Then, add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0085] Example 6

[0086] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0087] S1: Weigh parts by weight respectively, 1.5 parts of Gynostemma pentaphyllum, 3.5 parts of Polygonatum sibiricum, 4 parts of Ganoderma lucidum, 3 parts of Polygonatum multiflorum, 4 parts of Panax notoginseng, 3.5 parts of Gastrodia elata, 4 parts of Eucommia ulmoides, 5 parts of Polygonatum odoratum, 5 parts of Fructus hemp and 2.5 parts of Licorice, grind them to a particle size of less than 1 mm, and mix to obtain medicinal material powder A.

[0088] S2: Decoct the medicinal powder A with water in an amount of 15 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 22 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 1.5 to obtain medicinal solution D.

[0089] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.5:0.15:0.25, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 8.5 times the mass of brine with a concentration of 8.5g / 100mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 37°C for 54h to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 3.5h, and the filtrate is filtered to obtain the filtrate, and the filtrate is concentrated to a relative density of 1.5 extract to obtain the medicinal solution G.

[0090] Wherein, the baking temperature of the baked highland barley bran powder is 170° C., and the baking time is 55 min.

[0091] The amount of amylase added is 0.12% of the mass of the fermentation liquid F, and the amylase activity unit is 2500 U / g. The amount of xylanase added is 0.12% of the mass of the fermentation liquid F, and the xylanase activity unit is 40000 U / g.

[0092] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 2: 0.8: 0.2: 0.2: 0.04: 0.05: 0.05: 0.6. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After the temperature drops to 40-42°C, add liquid D and liquid G and mix evenly. Then, add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0093] Example 7

[0094] A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood comprises the following steps:

[0095] S1: Weigh parts by weight respectively, 2 parts of Gynostemma pentaphyllum, 5 parts of Polygonatum sibiricum, 5 parts of Ganoderma lucidum, 4 parts of Polygonatum multiflorum, 5 parts of Panax notoginseng, 5 parts of Gastrodia elata, 1 part of Eucommia ulmoides, 4.5 parts of Polygonatum odoratum, 4 parts of Cannabis sativa seed and 5 parts of Licorice, grind them to a particle size of less than 1 mm, and mix them to obtain medicinal material powder A.

[0096] S2: Decoct the medicinal powder A with water in an amount of 10 times the mass of the medicinal powder A, and keep the decoction temperature at boiling point for 36 minutes. Filter to obtain residue B and filtrate C, and concentrate the filtrate to an extract with a relative density of 2.0 to obtain medicinal solution D.

[0097] S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1:0.45:0.12:0.25, the filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, 8 times the mass of brine with a concentration of 9.5g / 100mL is added to the mixture E, and lactic acid bacteria is added to carry out anaerobic fermentation at 36.8°C for 54h to obtain a fermentation liquid F, and then amylase and xylanase are added to the fermentation liquid F for enzymolysis for 4.5h, and the filtrate is filtered to obtain a filtrate, and the filtrate is concentrated to a relative density of 2.0 to obtain an extract to obtain a medicinal solution G.

[0098] Wherein, the baking temperature of the baked highland barley bran powder is 190° C., and the baking time is 45 min.

[0099] The amount of amylase added is 0.1% of the mass of the fermentation liquid F, and the amylase activity unit is 2300 U / g; the amount of xylanase added is 0.08% of the mass of the fermentation liquid F, and the xylanase activity unit is 50000 U / g.

[0100] S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = 4:1.5:0.4:0.4:0.08:0.08:0.08:0.7. First, boil the fruit flavor, sweetener and sodium alginate in water to dissolve them. After cooling to 40°C, add liquid D and liquid G and mix evenly. Then add bovine liver protein peptide and sheep liver phosphorylated peptide, and mix to obtain Huang He oral liquid.

[0101] 1. Safety test:

[0102] (1) Acute toxicity test:

[0103] The oral liquids of Examples 2 to 7 were respectively administered to the test mice by gavage at twice the normal dose, and were administered three times within 24 hours, each time at an interval of 4 hours, and the cumulative total amount of the drug reached 30 mL. Within 7 days after administration, the mice were active, eating, and excreting normally, with shiny fur and healthy bodies. The test mice were killed on the 8th day, and autopsies, organ coefficients, and histological examinations were performed, and the results were normal, indicating that the oral liquids of Examples 2 to 7 had no acute toxic reactions.

[0104] (2) Long-term toxicity test:

[0105] The oral liquids of Examples 2 to 7 were continuously gavaged to test mice at a dose twice the normal dose for 3 months, and the external physical symptoms, behavioral activities, secretions, fecal characteristics, etc. were continuously observed. After 3 months, the test mice were killed for autopsy, organ coefficients and histological examinations, and the results showed no abnormalities, indicating that the oral liquids of Examples 2 to 7 had no long-term toxic reactions and the compositions were safe and non-toxic.

[0106] The selection of raw materials is within the range of types allowed for drug addition.

[0107] 2. Clinical Trials:

[0108] 120 subjects with qi and blood deficiency and liver and kidney sub-health symptoms were selected and randomly divided into 6 groups (20 subjects in each group), and the subjects were respectively given the oral liquids of Examples 2 to 7. The age, course of disease, etc. of the 6 groups of subjects were not significantly different (p>0.05), and were comparable.

[0109] Take once a day in the morning and evening, 5 ml each time after dilution, observe and count the evaluation results of the subjects as follows:

[0110]

[0111]

[0112]

[0113]

[0114] The above results indicate that the therapeutic effects of the oral solutions of Examples 2 to 8 are comparable to that of Example 1.

Claims

1. A method for preparing Huanghe oral liquid for replenishing qi and nourishing blood, characterized in that: The method comprises the following steps: S1: Weigh 1 to 5 parts by weight of Gynostemma pentaphyllum, Polygonatum sibiricum, Ganoderma lucidum, Polygonatum multiflorum, Panax notoginseng, Gastrodia elata, Eucommia ulmoides, Polygonatum odoratum, Fructus hemp and Licorice respectively, grind and mix to obtain medicinal material powder A; S2: decoct the medicinal powder A with water in an amount of 8 to 15 times the mass of the medicinal powder A, and keep the decoction temperature at boiling water for 20 to 40 minutes, filter to obtain a residue B and a filtrate C, and concentrate the filtrate C to a relative density of 1.5 to 2.0 to obtain a medicinal solution D; S3: According to the mass ratio, filter residue B: barley flour: barley bran powder: roasted highland barley bran powder = 1: (0.3-0.5): (0.05-0.2): (0.1-0.3), filter residue B, barley flour, barley bran powder and roasted highland barley bran powder are mixed to obtain a mixture E, salt water and lactic acid bacteria are added to the mixture E, and anaerobic fermentation is carried out at 36-37° C. for 48-60 h to obtain a fermentation liquid F, and then 0.05-0.15% by mass of amylase and 0.05-0.15% by mass of xylanase are added to the fermentation liquid F for enzymolysis for 3-5 h, and a filtrate is obtained by filtering, and the relative density of the concentrated filtrate reaches 1.5-2.0 to obtain a liquid G; The baking temperature of the baked highland barley bran powder is 150-200° C., and the baking time is 30-60 min; the concentration of the brine is 8-10 g / 100 mL, and the amount of the brine added is 6-10 times the mass of the mixture E; the activity unit of the amylase is 2000-2500 U / g; the activity unit of the xylanase is 40000-50000 U / g; S4: According to the mass ratio, liquid D: liquid G: bovine liver protein peptide: sheep liver phosphorylated peptide: fruit flavor: sweetener: sodium alginate: water = (1-5): (0.5-2): (0.1-0.5): (0.1-0.5): (0.01-0.1): (0.01-0.1): (0.05-0.1): (0.5-1), first add water to boil the fruit flavor, sweetener and sodium alginate to dissolve, reduce the temperature to 40-42°C, then add liquid D and liquid G and mix evenly, then add bovine liver protein peptide and sheep liver phosphorylated peptide, mix to obtain Huang He oral liquid.

2. The method for preparing the Qi-invigorating and blood-nourishing Huanghe oral liquid according to claim 1, characterized in that: In S1, the particle size of the crushed material is less than 1 mm.

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