A compound quaternary ammonium salt bactericidal disinfectant and preparation method thereof

Abstract

The present invention relates to the technical field of medical disinfectants, and in particular to a compound quaternary ammonium salt bactericidal disinfectant and a preparation method thereof. The compound quaternary ammonium salt bactericidal disinfectant comprises a composite quaternary ammonium salt, an amine substance, a nonionic surfactant, a plant-derived antibacterial agent, hydroxyethyl cellulose, anhydrous ethanol, and water. The present invention also provides a preparation method thereof. Compared with the prior art, the compound quaternary ammonium salt bactericidal disinfectant prepared by the present invention simultaneously introduces amine substances, double-chain quaternary ammonium salts, single-chain quaternary ammonium salts, plant extracts, and plant extract microcapsules, which have a synergistic effect with each other, further enhance the disinfection effect, and have a wider sterilization range; the plant extract microcapsules are introduced to extend the antibacterial, disinfecting, and deodorizing properties, and the formula is mild, will not irritate the human body, is harmless to the human body, and is healthy and environmentally friendly.

Description

Technical Field

[0001] The invention relates to the technical field of medical disinfectants, and in particular to a compound quaternary ammonium salt bactericidal disinfectant and a preparation method thereof. Background Art

[0002] Disinfectants are mainly a method of reducing the risk of cross-contamination of surface contact through process disinfection. The commonly used disinfectants on the market are inorganic chlorine salts, such as sodium hypochlorite, bleaching powder, bleaching essence, dichloroisocyanuric acid, trichloroisocyanuric acid, etc.; alcohol-containing, such as 75% alcohol, isopropyl alcohol, compound alcohol, etc.; strong oxidants, such as hydrogen peroxide, etc. Although these disinfectants can have a good disinfection and sterilization effect, their effectiveness is very short-lived, their long-lasting antibacterial performance is poor, and they have a strong pungent odor.

[0003] Quaternary ammonium compound disinfectants can adsorb negatively charged bacteria, have good bactericidal effects, and are widely used internationally.

[0004] CN116114715A discloses a low-temperature quaternary ammonium salt disinfectant for animals and a preparation method thereof, comprising a quaternary ammonium salt disinfectant, wormwood, wild chrysanthemum, garlic, bupleurum, a deodorant, an antifreeze, 70% ethanol and water, wherein the weight ratio is as follows: the proportion of the quaternary ammonium salt disinfectant is set to 30-50 parts, the proportion of the wormwood is set to 5-10 parts, the proportion of the wild chrysanthemum is set to 3-5 parts, the proportion of the garlic is set to 6-15 parts, the proportion of the bupleurum is set to 4-9 parts, the proportion of the deodorant is set to 5-12 parts, the proportion of the antifreeze is set to 2-5 parts, the proportion of the 70% ethanol is set to 10-20 parts, and the balance is water. Compared with ordinary disinfectants, the low-temperature quaternary ammonium salt disinfectant can not freeze, solidify or fail in a sub-zero environment, and can always maintain a liquid state, can be sprayed for disinfection, effectively kill Escherichia coli, Staphylococcus aureus, etc., and the sterilization rate can reach 99.99%. The use of a variety of Chinese patent medicine prescriptions improves the safety of the disinfectant, has no odor, avoids harming the physical and mental health of animals, and has a good disinfection effect.

[0005] Therefore, there is an urgent need for a composite quaternary ammonium salt bactericidal disinfectant that is highly efficient, widely bactericidal, durable, safe, non-toxic or has low toxicity and is non-irritating. Summary of the invention

[0006] To achieve the above object, the present invention provides a composite quaternary ammonium salt bactericidal disinfectant, characterized by: composite quaternary ammonium salt, amine substances, nonionic surfactant, hydroxyethyl cellulose, anhydrous ethanol, and water.

[0007] Further preferably, a composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, measured by mass percentage: 0.2-2% composite quaternary ammonium salt, 0.1-1% amine substance, 0.1-5% nonionic surfactant, 0.1-2% hydroxyethyl cellulose, 12-16% anhydrous ethanol, and the balance is water.

[0008] The nonionic surfactant is a mixture of one or more of dodecyl dihydroxyethyl amine oxide and coconut oil alkyl bishydroxyethyl amine oxide;

[0009] The amine substance is laurylamine dipropylene diamine.

[0010] Amine substances mainly form positively charged substances in water, adsorb on the negatively charged surface of microorganisms, form micelles, and enter the lipid layer of the cell membrane through osmosis, changing the permeability of the membrane, which can lead to rupture of the cell membrane and extravasation of cell contents, thereby killing the microorganisms; at the same time, they can change the activity of multiple enzymes and regulate changes in the expression of related genes, hinder cell metabolism, and have a good bactericidal effect.

[0011] The composite quaternary ammonium salt is a mixture of a double-chain quaternary ammonium salt and a single-chain quaternary ammonium salt;

[0012] The double-chain quaternary ammonium salt is at least one of decanylmethylammonium chloride, decanylmethylammonium bromide, dioctyldimethylammonium chloride, dioctadecyldimethylammonium chloride, didodecyldimethylammonium chloride, ditetradecyldimethylammonium chloride, dihexadecyldimethylammonium chloride, and 2-(2-phenoxyethoxy)ethyltrimethylammonium chloride.

[0013] The single-chain quaternary ammonium salt is at least one of benzalkonium bromide, benzalkonium chloride, benzethonium chloride, tetradecyl dimethyl benzyl ammonium chloride, dodecyl dimethyl benzyl ammonium chloride, octadecyl trimethyl ammonium chloride, and dodecyl trimethyl ammonium chloride.

[0014] Furthermore, the composite quaternary ammonium salt is prepared by mixing decanylmethylammonium chloride and benzethonium chloride in a mass ratio of (1-3):(1-3).

[0015] As a common component of disinfectants, double-chain quaternary ammonium salt compounds can change the permeability of bacterial spore membranes, allowing components inside bacteria to penetrate outward, hindering cell metabolism and thus killing bacteria; the composite quaternary ammonium salt proposed by the present invention is composed of single-chain and double-chain quaternary ammonium salts, and its disinfection and sterilization performance is better than that of single double-chain and double-chain quaternary ammonium salts; the double-chain quaternary ammonium salt contains two long-chain hydrophobic groups and two positively charged N + , through induction, the positive charge density of quaternary nitrogen is increased, and the adsorption capacity of the disinfectant on the bacterial surface is increased, thereby changing the permeability of the membrane and causing the bacteria to rupture. Therefore, it can not only effectively expand the bactericidal spectrum of the disinfectant, but also enhance the disinfection effect; the non-ionic surfactant molecules are inserted between the polar head groups of the ionic surfactant by means of hydrophobic effects and interactions between polar head groups, thereby improving the charge density, synergizing the bactericidal effect of quaternary ammonium salts and improving their effects.

[0016] However, long-term use of a single disinfectant for surface disinfection will also cause antibacterial resistance. In order to further improve its antibacterial effect, plant-based antibacterial and bactericidal preparations are introduced, and quaternary ammonium salts are combined with natural biological antibacterial agents to improve environmental problems, while improving antibacterial and disinfection problems, and improving antibacterial resistance problems.

[0017] Further preferably, a composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, measured by mass percentage: 0.2-2% composite quaternary ammonium salt, 0.1-1% amine substance, 0.1-5% nonionic surfactant, 0.1-10% plant-derived antibacterial agent, 0.1-2% hydroxyethyl cellulose, 12-16% anhydrous ethanol, and the balance is water.

[0018] The botanical antibacterial agent may be a plant extract;

[0019] Furthermore, the plant-derived antibacterial agent may also be a plant extract microcapsule;

[0020] Further preferably, the botanical antibacterial agent is a mixture of plant extracts and plant extract microcapsules; the botanical antibacterial agent is a mixture of plant extracts and plant extract microcapsules in a mass ratio of 1:(1-3);

[0021] The plant extract is at least one of tea tree extract, sweet orange peel extract, mint extract, clove extract, lemon extract, and raspberry extract;

[0022] Raspberry (Rubus Chingii Hu) is a woody plant of the genus Rubus in the Rosaceae family. It was recorded in the "Shennong Herbal Classic" as early as the Qin and Han Dynasties. Raspberry extract mainly consists of raspberry ketone, flavonoids, and phenolic acid compounds, which have antioxidant, antibacterial, and anti-inflammatory effects; among them, raspberry ketone has the characteristic aroma of raspberry, it has the effect of enhancing flavor and sweetness, giving it a fruity and sweet aroma, which can make the aroma last long and effectively weaken and inhibit the emission of odorous substances.

[0023] Sweet orange peel extract contains a large amount of sweet orange oil, which has a calming effect and a sweet orange scent. It can be used as a fragrance, purify and improve the air, and can also drive away tension and stress, and improve insomnia caused by anxiety. Most of the effective molecules in raspberry extract, sweet orange peel extract, mint extract, clove extract, lemon extract, and tea tree extract contain active groups that can react with odor molecules. Multiple raw materials work synergistically to achieve good deodorizing and sterilizing properties. Plant extracts have a deodorizing effect on the environment during the sterilization process. Plant extracts have a strong adsorption effect and can combine with harmful gases such as ammonia, hydrogen sulfide, and methyl indole to reduce their concentration, thereby improving odor and playing a certain role in deodorization, disinfection, and sterilization.

[0024] Plant extracts contain a large number of active ingredients with antibacterial, deodorizing and deodorizing properties, but their active substances are easily oxidized and volatilized and cannot exert their effects for a long time, and their performance needs to be further improved.

[0025] The preparation method of the plant extract microcapsule comprises:

[0026] (1) adding 1-3.5 parts by weight of hydroxyapatite to 20-30 parts by weight of water and ultrasonically treating the mixture for 1-2 hours to obtain a hydroxyapatite dispersion, wherein the ultrasonic frequency is 15-25 kHz and the ultrasonic power is 100-500 W; adding 7-8 parts by weight of sodium hydroxide and 10-15 parts by weight of urea to 90-100 parts by weight of water and mixing the mixture evenly, and then precooling the mixture in a -15 to -10°C refrigerator for 10-30 minutes, adding 5-6 parts by weight of microcrystalline cellulose, stirring the mixture at 100-500 r / min for 10-30 minutes, and placing the mixture at 0°C; finally adding 3-5 parts by weight of epichlorohydrin, stirring the mixture at 100-500 r / min for 30-60 minutes, and drying the mixture at 50-60°C for 12-24 hours to obtain a gel, washing the mixture with water until neutral, and then freezing the mixture at -60 to -50°C for 12-24 hours, and finally freeze-drying the mixture to obtain an aerogel;

[0027] (2) crushing the aerogel obtained above into 1-5 μm, then adding the aerogel powder and the plant extract into a 60-80 wt % ethanol aqueous solution and mixing for 10-30 min, filtering and drying to obtain an aerogel loaded with the plant extract, wherein the mass ratio of the aerogel powder, the plant extract and the ethanol aqueous solution is (1-2):1:(5-15);

[0028] (3) adding 1-3 parts by weight of a stabilizer, 2-4 parts by weight of gelatin, and 5-15 parts by weight of aerogel loaded with plant extracts to 50-100 parts by weight of water and mixing evenly, then adding 100-200 parts by weight of a 1-3wt% sodium alginate aqueous solution and stirring evenly to obtain a mixed dispersion system; mixing 1-3wt% chitosan quaternary ammonium salt aqueous solution and 1-2wt% calcium chloride aqueous solution at a mass ratio of (1-2):1 to obtain a chitosan quaternary ammonium salt-calcium chloride mixed solution; mixing the mixed dispersion system and the chitosan quaternary ammonium salt-calcium chloride mixed solution evenly at a volume ratio of 1:1 to obtain a mixed solution; adding 1-3 times the volume of water to the mixed solution and stirring at 100-500r / min for 20-30min, finally standing at room temperature for 1-3h, filtering, and drying to obtain plant extract microcapsules.

[0029] The microcrystalline cellulose and hydroxyapatite used in the present invention have the characteristics of low cost, excellent biocompatibility, environmental friendliness, and biodegradability. The present invention uses hydroxyapatite and microcrystalline cellulose as raw materials to prepare aerogels with a honeycomb structure. Hydroxyapatite is evenly distributed in the microcrystalline cellulose to effectively improve the pore size and specific surface area of ​​the aerogels, and improves their adsorption performance for plant extracts. However, the aerogels loaded with plant extracts have poor compatibility in disinfectants, are easy to precipitate, and the plant extracts are easy to desorb and disperse in the disinfectant, affecting their sustained release, long-term antibacterial, deodorizing, and disinfecting performance. Further, the aerogels loaded with plant extracts are used to prepare aerogels with a honeycomb structure. The aerogel is used as the matrix, and sodium alginate, chitosan quaternary ammonium salt, and calcium chloride are used to wrap it to improve the sustained release performance and prolong the antibacterial, disinfecting, and deodorizing effects; the plant extract microgel prepared by the present invention can interact well with the effective ingredients in the disinfectant, and its wall material slowly decomposes in the disinfectant, has long-term stability, and at the same time makes the disinfectant have lasting antibacterial, disinfecting, deodorizing and other effects; the plant-derived antibacterial agent used in the present invention is synergistically composed of plant extracts and plant extract microcapsules, and the plant extract microcapsules can make up for the disappearance or reduction of the performance of the plant extract after volatilization. During the disinfection process, the active substances of the plant extracts in the disinfectant are easily oxidized and volatilized, and cannot play their role for a long time. The plant extracts treated with the sustained release of the plant extract microcapsules can make up for their losses and continue to play the antibacterial and deodorizing effects.

[0030] The invention also provides a method for preparing the compound quaternary ammonium salt bactericidal disinfectant.

[0031] The invention discloses a preparation method of a compound quaternary ammonium salt bactericidal disinfectant. The preparation method comprises the following steps: according to a formula, a compound quaternary ammonium salt, an amine substance, a nonionic surfactant, a plant-derived antibacterial agent, hydroxyethyl cellulose, anhydrous ethanol and water are stirred and mixed uniformly to obtain the compound quaternary ammonium salt bactericidal disinfectant.

[0032] The composite quaternary ammonium salt bactericidal disinfectant proposed by the present invention has the advantages of being easy to use, non-toxic, non-irritating, and non-corrosive.

[0033] The open-ended terms such as “comprising”, “including”, etc. used in the present invention also cover the situation of “consisting of…”

[0034] When using the quaternary ammonium salt disinfectant of the present invention, it is also necessary to pay attention to:

[0035] (1) External disinfectants should not be taken orally. Keep out of the reach of children.

[0036] (2) Avoid mixing with anionic surfactants and soaps.

[0037] (3) Store in a cool, dry place, away from light, sealed and moisture.

[0038] Beneficial effects of the present invention:

[0039] 1. Compared with the prior art, the compound quaternary ammonium salt bactericidal disinfectant prepared by the present invention simultaneously introduces amine substances, double-chain quaternary ammonium salts, single-chain quaternary ammonium salts, plant extracts, and plant extract microcapsules, which act synergistically with each other, further enhancing the disinfection effect and having a wider bactericidal range; the introduction of plant extract microcapsules prolongs the antibacterial, disinfecting, and deodorizing properties.

[0040] 2. Compared with the prior art, the present invention introduces raspberry extract and tea tree extract. Most of the effective molecules in raspberry extract and tea tree extract contain active groups, which can react with odor molecules. Multiple raw materials work synergistically to achieve good deodorization and sterilization performance. Plant extracts have a deodorizing effect on the environment during the sterilization process. Plant extracts have a strong adsorption effect and can combine with harmful gases such as ammonia, hydrogen sulfide, and methyl indole to reduce their concentration, thereby improving odor and playing a certain deodorizing, disinfecting, and sterilization role. Raspberry extract, tea tree extract and plant extract microcapsules are mixed and act synergistically. Plant extract microcapsules can make up for the problem of disappearance or reduction of performance after the plant extract volatilizes.

[0041] 3. The disinfectant prepared by the present invention has a mild formula, will not irritate the human body, is harmless to the human body, is healthy and environmentally friendly. DETAILED DESCRIPTION

[0042] The endpoints and any values ​​of the ranges disclosed in the present invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be regarded as specifically disclosed in the present invention.

[0043] Introduction of some raw materials used in the embodiments of the present invention:

[0044] Hydroxyapatite, particle size 100 nm, product number 170508161249, (Kramer) Shanghai Puzhen Biotechnology Co., Ltd.

[0045] The preparation method of the tea tree extract is as follows: wash, dry and crush tea leaves and branches, add a mixed solution of water and anhydrous ethanol in a volume ratio of 5:95, and reflux extract twice, add 6 times the weight of the tea tree for the first time, and reflux for 3 hours; add 3 times the weight of the tea tree for the second time, and reflux for 2 hours; combine the two extracts, concentrate, and obtain the tea tree extract.

[0046] The preparation method of raspberry extract comprises the following steps: washing, drying and crushing raspberries, adding a mixed solution of water and anhydrous ethanol in a volume ratio of 10:90, and reflux extracting twice, adding 5 times the weight of raspberries for the first time and refluxing for 3 hours; adding 2 times the weight of raspberries for the second time and refluxing for 2 hours, combining the two extracts, concentrating, and obtaining raspberry extract;

[0047] Other raw materials not mentioned are common raw materials. The above content is only to help illustrate the present invention and should not be construed as a strict limitation of the present invention. Those skilled in the art can directly purchase or prepare the same / similar raw materials from the market.

[0048] The following will be combined with the specific embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] Example 1

[0050] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, measured by mass percentage: 0.5% of decanium chloride, 0.5% of benzethonium chloride, 0.6% of laurylamine dipropylene diamine, 1% of coconut oil alkyl bishydroxyethyl amine oxide, 4% of raspberry extract, 4% of tea tree extract, 1% of hydroxyethyl cellulose, 14% of anhydrous ethanol, and the balance is water.

[0051] Example 2

[0052] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, measured by mass percentage: 0.5% of decanium chloride, 0.5% of benzalkonium chloride, 0.6% of laurylamine dipropylene diamine, 1% of coconut oil alkyl bishydroxyethyl amine oxide, 4% of raspberry extract, 4% of tea tree extract, 1% of hydroxyethyl cellulose, 14% of anhydrous ethanol, and the balance is water.

[0053] Example 3

[0054] A composite quaternary ammonium salt bactericidal disinfectant, comprising the following raw materials, calculated by mass percentage: 0.5% decanium chloride, 0.5% benzethonium chloride, 0.6% laurylamine dipropylene diamine, 1% coconut oil alkyl bishydroxyethyl amine oxide, 8% plant extract microcapsules, 1% hydroxyethyl cellulose, 14% anhydrous ethanol, and the balance is water;

[0055] The preparation method of the plant extract microcapsule comprises:

[0056] (1) 3.1 parts by weight of hydroxyapatite was added to 25.4 parts by weight of water and ultrasonically treated for 2 hours to obtain a hydroxyapatite dispersion, wherein the ultrasonic frequency was 20 kHz and the ultrasonic power was 200 W; 7.5 parts by weight of sodium hydroxide and 12.2 parts by weight of urea were added to 92 parts by weight of water and mixed evenly, and then placed in a -13°C freezer for precooling for 20 minutes, and then 5.3 parts by weight of microcrystalline cellulose was added, stirred at 300 r / min for 30 minutes, placed at 0°C, and finally 3.8 parts by weight of epichlorohydrin was added, stirred at 300 r / min for 60 minutes, and dried at 55°C for 12 hours to obtain a gel, which was washed with water until neutral, and then placed in a -54°C freezer for 12 hours, and finally freeze-dried to obtain an aerogel;

[0057] (2) crushing the aerogel obtained above into 2 μm, then adding the aerogel powder and the plant extract into a 70 wt % ethanol aqueous solution and mixing for 30 min, filtering and drying to obtain an aerogel loaded with the plant extract, wherein the mass ratio of the aerogel powder, the plant extract and the ethanol aqueous solution is 1:1:10;

[0058] (3) Add 2 parts by weight of paraffin, 3 parts by weight of gelatin, and 10 parts by weight of aerogel loaded with plant extract to 100 parts by weight of water and mix them evenly, then add 200 parts by weight of 3wt% sodium alginate aqueous solution and stir them evenly to obtain a mixed dispersion system; 2.5wt% chitosan quaternary ammonium salt aqueous solution and 2wt% calcium chloride aqueous solution are mixed evenly at a mass ratio of 2:1 to obtain a chitosan quaternary ammonium salt-calcium chloride mixed solution; the mixed dispersion system and the chitosan quaternary ammonium salt-calcium chloride mixed solution are mixed evenly at a volume ratio of 1:1 to obtain a mixed solution; add 3 times the volume of water to the mixed solution and stir at 300r / min for 20min, finally stand at room temperature for 3h, filter and dry to obtain plant extract microcapsules; wherein the plant extract is a mixture of raspberry extract and tea tree extract at a mass ratio of 1:1.

[0059] Example 4

[0060] A composite quaternary ammonium salt bactericidal disinfectant is substantially the same as Example 3, except that:

[0061] Using aerogel loaded with plant extracts to replace plant extract microcapsules;

[0062] The method for preparing the aerogel loaded with plant extracts comprises:

[0063] (1) 3.1 parts by weight of hydroxyapatite was added to 25.4 parts by weight of water and ultrasonically treated for 2 hours to obtain a hydroxyapatite dispersion, wherein the ultrasonic frequency was 20 kHz and the ultrasonic power was 200 W; 7.5 parts by weight of sodium hydroxide and 12.2 parts by weight of urea were added to 92 parts by weight of water and mixed evenly, and then placed in a -13°C freezer for precooling for 20 minutes, and then 5.3 parts by weight of microcrystalline cellulose was added, stirred at 300 r / min for 30 minutes, placed at 0°C, and finally 3.8 parts by weight of epichlorohydrin was added, stirred at 300 r / min for 60 minutes, and dried at 55°C for 12 hours to obtain a gel, which was washed with water until neutral, and then placed in a -54°C freezer for 12 hours, and finally freeze-dried to obtain an aerogel;

[0064] (2) The aerogel obtained above was crushed into 2 μm, and then the aerogel powder and the plant extract were added to a 70 wt % ethanol aqueous solution and mixed for 30 min, filtered and dried to obtain an aerogel loaded with a plant extract, wherein the mass ratio of the aerogel powder, the plant extract and the ethanol aqueous solution was 1:1:10.

[0065] Example 5

[0066] A composite quaternary ammonium salt bactericidal disinfectant is substantially the same as Example 3, except that:

[0067] The preparation method of the plant extract microcapsule comprises:

[0068] 2 parts by weight of paraffin, 3 parts by weight of gelatin and 5 parts by weight of plant extract are added to 100 parts by weight of water and mixed evenly, and then 200 parts by weight of 3wt% sodium alginate aqueous solution are added and stirred evenly to obtain a mixed dispersion system; 2.5wt% chitosan quaternary ammonium salt aqueous solution and 2wt% calcium chloride aqueous solution are mixed evenly at a mass ratio of 2:1 to obtain a chitosan quaternary ammonium salt-calcium chloride mixed solution; the mixed dispersion system and the chitosan quaternary ammonium salt-calcium chloride mixed solution are mixed evenly at a volume ratio of 1:1 to obtain a mixed solution; 3 times the volume of water is added to the mixed solution and stirred at 300r / min for 20min, and finally allowed to stand at room temperature for 3h, filtered and dried to obtain plant extract microcapsules; wherein the plant extract is a mixture of raspberry extract and tea tree extract at a mass ratio of 1:1.

[0069] Example 6

[0070] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, measured by mass percentage: 0.5% decanium chloride, 0.5% benzethonium chloride, 0.6% laurylamine dipropylene diamine, 1% coconut oil alkyl bishydroxyethyl amine oxide, 1% raspberry extract, 1% tea tree extract, 6% plant extract microcapsules, 1% hydroxyethyl cellulose, 14% anhydrous ethanol, and the balance is water; wherein the preparation method of the plant extract microcapsules is consistent with that of Example 3.

[0071] Example 7

[0072] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, measured by mass percentage: 0.5% of decanium chloride, 0.5% of benzethonium chloride, 1% of coconut oil alkyl bishydroxyethyl amine oxide, 4% of raspberry extract, 4% of tea tree extract, 1% of hydroxyethyl cellulose, 14% of anhydrous ethanol, and the balance is water.

[0073] Comparative Example 1

[0074] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, calculated by mass percentage: 0.5% of decanium chloride, 0.5% of benzethonium chloride, 0.6% of laurylamine dipropylene diamine, 1% of coconut oil alkyl bishydroxyethyl amine oxide, 8% of raspberry extract, 1% of hydroxyethyl cellulose, 14% of anhydrous ethanol, and the balance is water.

[0075] Comparative Example 2

[0076] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, calculated by mass percentage: 0.5% of decanium chloride, 0.5% of benzethonium chloride, 0.6% of laurylamine dipropylene diamine, 1% of coconut oil alkyl bishydroxyethyl amine oxide, 8% of tea tree extract, 1% of hydroxyethyl cellulose, 14% of anhydrous ethanol, and the balance is water.

[0077] Comparative Example 3

[0078] A composite quaternary ammonium salt bactericidal disinfectant comprises the following raw materials, calculated by mass percentage: 0.5% of decanium chloride, 0.5% of benzethonium chloride, 1% of coconut oil alkyl bishydroxyethyl amine oxide, 4% of raspberry extract, 4% of tea tree extract, 1% of hydroxyethyl cellulose, and the balance is water.

[0079] Test Example 1

[0080] The test was carried out with reference to 2.1.1 of Technical Specification for Disinfection (2002 Edition), and Escherichia coli 8099, Staphylococcus aureus ATCC6538, Candida albicans ATCC10231, and Pseudomonas aeruginosa ATCC15442 were selected as tests. The antibacterial test was carried out on the disinfectants of the embodiments and comparative examples. The ratio of the number of bacteria before and after disinfection / the change in the titer of the virus can be used to calculate the bactericidal rate and the killing rate. The test was repeated 3 times each, and the average value was shown in Table 1.

[0081] Table 1 Killing test results of composite quaternary ammonium salt bactericidal disinfectant

[0082]

[0083]

[0084] The composite quaternary ammonium salt proposed by the present invention is composed of single-chain and double-chain quaternary ammonium salts, and its disinfection and sterilization performance is better than that of single double-chain and double-chain quaternary ammonium salts; the double-chain quaternary ammonium salt contains two long-chain hydrophobic groups and two positively charged N + , through induction, the positive charge density of quaternary nitrogen is increased, the adsorption capacity of disinfectant on the bacterial surface is increased, thereby changing the permeability of the membrane and rupturing the bacterial body, so that not only the bactericidal spectrum of the disinfectant can be effectively expanded, but also the disinfection effect can be enhanced; the nonionic surfactant molecules are inserted between the polar head groups of the ionic surfactant by means of hydrophobic interaction and the interaction between the polar head groups, improving the charge density, and playing a synergistic role in the bactericidal effect of quaternary ammonium salts, improving its effect. However, long-term use of a certain disinfectant alone for surface disinfection will also produce antibacterial resistance, so the technical scheme of Example 1 is obtained by introducing botanical antibacterial and bactericidal preparations, and quaternary ammonium salts are combined with natural biological antibacterial agents to improve environmental problems, improve antibacterial and disinfection problems at the same time, and improve antibacterial resistance problems. The quaternary ammonium salt bactericidal disinfectant prepared in Example 1 reaches 99.99% in sterilization and disinfection after 1 day of disinfection. The effective molecules introduced in raspberry extract and tea tree extract in Example 1 mostly contain active groups, which can act on odor molecules, and multiple raw materials work synergistically to achieve good deodorization and bactericidal performance. Plant extracts have a deodorizing effect on the environment during the sterilization process. Plant extracts have a strong adsorption effect and can combine with harmful gases such as ammonia, hydrogen sulfide, and methyl indole to reduce their concentration, thereby improving odor and playing a certain role in deodorization, disinfection, and sterilization. In order to improve the problem that active substances are easily oxidized and volatilized and cannot play their role for a long time, the microcrystalline cellulose and hydroxyapatite used in the present invention are low in cost, have excellent biocompatibility, environmental friendliness, and are easy to degrade. The present invention uses hydroxyapatite and microcrystalline cellulose as raw materials to prepare aerogels with a honeycomb structure. Hydroxyapatite is evenly distributed in microcrystalline cellulose to effectively improve the pore size and specific surface area of ​​the aerogel, and improve its adsorption performance for plant extracts; however, the aerogel loaded with plant extracts has poor compatibility in disinfectants, is easy to precipitate, and the plant extracts It is easy to desorb and disperse in the disinfectant, affecting its sustained release, long-term antibacterial, deodorizing and disinfecting properties; further, the aerogel loaded with plant extracts is used as a matrix, and sodium alginate, chitosan quaternary ammonium salt and calcium chloride are used to wrap it, so as to improve the sustained release performance and prolong the antibacterial, disinfecting and deodorizing effects; the plant extract microgel prepared by the present invention can interact well with the effective ingredients in the disinfectant, and its wall material is slowly decomposed in the disinfectant, with long-term stability, and at the same time, the disinfectant has lasting antibacterial, disinfecting, deodorizing and other effects, thereby obtaining the technical scheme of Example 2, and improving the bactericidal / bacteriostatic effect.

[0085] The plant-derived antibacterial agent used in Example 5 is composed of a mixture of plant extracts and plant extract microcapsules. The plant extract microcapsules can make up for the problem of disappearance or degradation of performance after the plant extracts volatilize, while maintaining long-lasting bactericidal / antibacterial effects and improving air quality.

[0086] Test Example 2

[0087] At (30±1)℃, the disinfectant is effective in removing ammonia and hydrogen sulfide unpleasant gases.

[0088] The initial concentration of ammonia and hydrogen sulfide was 60 ppm, recorded as 100%, and the final concentration and content of hydrogen sulfide were tested 120 minutes after the addition of the prepared liquid.

[0089] Table 2 Test results of odor removal

[0090]

[0091] In Example 1, effective molecules introduced into raspberry extract and tea tree extract mostly contain active groups, which can react with odor molecules, and multiple raw materials work synergistically to achieve good deodorization and bactericidal performance. Plant extract has a deodorizing effect on the environment during the sterilization process, and plant extract has a strong adsorption effect, which can be combined with harmful gases such as ammonia, hydrogen sulfide, and methyl indole to reduce its concentration, thereby improving odor and playing a certain deodorizing, disinfecting, and sterilizing effect. The compound quaternary ammonium salt bactericidal disinfectant prepared by the present invention simultaneously introduces double-chain quaternary ammonium salt, single-chain quaternary ammonium salt, plant extract, and plant extract microcapsule substances to synergize with each other, further strengthen the disinfection effect, and have a wider sterilization range; plant extract microcapsules are introduced to extend antibacterial, disinfecting, and deodorizing performances.

[0092] Test Example 3

[0093] Select 14 New Zealand rabbits weighing 2-3kg, regardless of gender. Refer to the 2002 edition of the "Technical Specifications for Disinfection" and use New Zealand rabbits for skin irritation testing; 24 hours before the test, cut the hair on both sides of the animal's back within a range of 3cm 2 The next day, drop 0.2 mL of the tested disinfectant onto a 2.5 cm 2 The test substance was applied to the skin surface of one side on two layers of gauze of different sizes, and then covered with a layer of non-irritating oil paper and fixed with a non-irritating adhesive tape. The other side was used as a blank control (or solvent control) for 4 hours, and then the residual test substance was washed off with warm water; the local skin reaction was observed and scored 1 hour, 24 hours, and 48 hours after the test substance was removed. The results showed that the disinfectant prepared in Examples 1-7 acted on the rabbit skin for 4 hours and did not cause animal skin irritation reaction, the skin irritation index was <0.4, and the irritation intensity was non-irritating.

[0094] The disinfectant prepared by the invention has a mild formula, does not cause irritation to the human body, is harmless to the human body, is healthy and environmentally friendly.

[0095] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A composite quaternary ammonium salt bactericidal disinfectant, characterized in that: The raw materials include, by mass percentage, 0.2-2% of composite quaternary ammonium salt, 0.1-1% of amine substance, 0.1-5% of nonionic surfactant, 0.1-2% of hydroxyethyl cellulose, 12-16% of anhydrous ethanol, and the balance is water; Also includes 0.1-10% plant-derived antibacterial agent; The botanical antibacterial agent is a mixture of plant extracts and plant extract microcapsules; the botanical antibacterial agent is a mixture of plant extracts and plant extract microcapsules in a mass ratio of 1:(1-3); The preparation method of the plant extract microcapsule comprises: (1) adding 1-3.5 parts by weight of hydroxyapatite to 20-30 parts by weight of water and ultrasonically treating the mixture for 1-2 hours to obtain a hydroxyapatite dispersion, wherein the ultrasonic frequency is 15-25 kHz and the ultrasonic power is 100-500 W; adding 7-8 parts by weight of sodium hydroxide and 10-15 parts by weight of urea to 90-100 parts by weight of water and mixing the mixture evenly, and then precooling the mixture in a -15 to -10°C refrigerator for 10-30 minutes, adding 5-6 parts by weight of microcrystalline cellulose, stirring the mixture at 100-500 r / min for 10-30 minutes, and placing the mixture at 0°C; finally adding 3-5 parts by weight of epichlorohydrin, stirring the mixture at 100-500 r / min for 30-60 minutes, and drying the mixture at 50-60°C for 12-24 hours to obtain a gel, washing the mixture with water until neutral, and then freezing the mixture at -60 to -50°C for 12-24 hours, and finally freeze-drying the mixture to obtain an aerogel; (2) crushing the aerogel obtained above into 1-5 μm, then adding the aerogel powder and the plant extract into a 60-80 wt % ethanol aqueous solution and mixing for 10-30 min, filtering and drying to obtain an aerogel loaded with the plant extract, wherein the mass ratio of the aerogel powder, the plant extract and the ethanol aqueous solution is (1-2):1:(5-15); (3) adding 1-3 parts by weight of a stabilizer, 2-4 parts by weight of gelatin, and 5-15 parts by weight of aerogel loaded with plant extracts to 50-100 parts by weight of water and mixing evenly, then adding 100-200 parts by weight of a 1-3wt% sodium alginate aqueous solution and stirring evenly to obtain a mixed dispersion system; mixing 1-3wt% chitosan quaternary ammonium salt aqueous solution and 1-2wt% calcium chloride aqueous solution in a mass ratio of (1-2):1 to obtain a chitosan quaternary ammonium salt-calcium chloride mixed solution; mixing the mixed dispersion system and the chitosan quaternary ammonium salt-calcium chloride mixed solution evenly in a volume ratio of 1:1 to obtain a mixed solution; adding 1-3 times the volume of water to the mixed solution and stirring at 100-500r / min for 20-30min, finally standing at room temperature for 1-3h, filtering, and drying to obtain plant extract microcapsules; The composite quaternary ammonium salt is prepared by mixing decylmethylammonium chloride and benzethonium chloride in a mass ratio of (1-3):(1-3); The amine substance is laurylamine dipropylene diamine; The plant extract is prepared by mixing raspberry extract and tea tree extract in a mass ratio of 1:1; The stabilizer is paraffin.

2. The composite quaternary ammonium salt bactericidal disinfectant as claimed in claim 1, characterized in that The nonionic surfactant is a mixture of one or more of dodecyl dihydroxyethyl amine oxide and coconut oil alkyl bishydroxyethyl amine oxide.

3. A method for preparing a composite quaternary ammonium salt bactericidal disinfectant as described in any one of claims 1-2, characterized in that: According to the formula, the composite quaternary ammonium salt, amine substances, nonionic surfactant, plant-derived antibacterial agent, hydroxyethyl cellulose, anhydrous ethanol and water are stirred and mixed uniformly to obtain the composite quaternary ammonium salt bactericidal disinfectant.

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