A bacterial agent for reducing methane emissions from ruminants, a preparation method thereof, and a compound feed containing the bacterial agent

Abstract

The present invention relates to the technical field of microbial agents. The present invention provides a microbial agent for reducing methane emissions from ruminants, a preparation method thereof, and a compound feed containing the microbial agent. The microbial agent comprises the following raw materials in parts by weight: 6 to 10 parts of Bacteroides fragilis powder, 3 to 5 parts of white Ruminococcus powder, 3 to 5 parts of yellow Ruminococcus powder, 4 to 6 parts of Bacillus subtilis powder, 2 to 4 parts of Acetobacter striata powder, and 10 to 12 parts of Lactobacillus plantarum powder. The microbial agent of the present invention can supplement beneficial microorganisms in the stomach of ruminants, increase the number of Bacteroides fragilis and white Ruminococcus, affect the concentration of metabolites myristic acid and palmitic acid, and thus reduce methane emissions. The prepared feed adjusts the nutritional structure of the diet, can reduce the fermentation of sugars in the rumen, inhibit the methane production pathway, and achieve synergistic effects with the microbial agent.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial agents, and in particular to a microbial agent for reducing methane emission of ruminants, a preparation method thereof, and a compound feed containing the microbial agent. Background Art

[0002] In the special digestive system structure of ruminants, the rumen is the main place for methane production. Depending on the substrate, there are four ways to produce methane: carbon dioxide reduction pathway, acetic acid dissimilation pathway, methanol disproportionate differentiation pathway, and formate oxidation pathway. The number of microbial communities in the rumen is huge, of which there are about 5,000 species of microorganisms, and the relationships are complex. Among these microorganisms, the largest number and most diverse are rumen bacteria. These microorganisms complete the fermentation process of rumen microorganisms through symbiosis, antagonism, competition, etc. to ensure the dynamic balance of the rumen environment. There are many reasons for the factors that affect the methane emissions of ruminants, including ruminant breeds, feed types and composition, feed intake, feed additives, and other factors.

[0003] In production and life, greenhouse gases emitted by the agricultural animal husbandry industry and the food industry account for 21-37% of the total greenhouse gas emissions, of which the amount of methane gas emitted by ruminants each year accounts for 28% of the methane emissions produced by human life. Therefore, it is particularly important to develop products and methods to reduce methane emissions from ruminants. Summary of the invention

[0004] The purpose of the present invention is to provide a bacterial agent for reducing methane emissions from ruminants, a preparation method thereof, and a compound feed containing the bacterial agent, so as to supplement beneficial microorganisms in the stomach of ruminants and reduce methane emissions.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a bacterial agent for reducing methane emissions from ruminants. The bacterial agent comprises the following raw materials in parts by weight: 6 to 10 parts of Bacteroides fragilis powder, 3 to 5 parts of white Ruminococcus powder, 3 to 5 parts of yellow Ruminococcus powder, 4 to 6 parts of Bacillus subtilis powder, 2 to 4 parts of Acetobacter striata powder and 10 to 12 parts of Lactobacillus plantarum powder.

[0007] Preferably, the bacterial agent comprises the following raw materials in parts by weight: 7 to 9 parts of Bacteroides fragilis powder, 3.5 to 4.5 parts of white Ruminococcus powder, 3.5 to 4.5 parts of yellow Ruminococcus powder, 4.5 to 5.5 parts of Bacillus subtilis powder, 2.5 to 3.5 parts of Acetobacter striata powder and 10.5 to 11.5 parts of Lactobacillus plantarum powder.

[0008] Preferably, the bacterial agent comprises the following raw materials in parts by weight: 8 parts of Bacteroides fragilis powder, 4 parts of white Ruminococcus powder, 4 parts of yellow Ruminococcus powder, 5 parts of Bacillus subtilis powder, 3 parts of Acetobacter membranaceus powder and 10 parts of Lactobacillus plantarum powder.

[0009] Preferably, the effective viable bacteria count of Bacteroides fragilis in the Bacteroides fragilis powder is 1.2 to 1.8×10 10 cfu / g;

[0010] The effective viable bacteria count of the white rumenococcus in the white rumenococcus powder is 3.0 to 3.6×10 10 cfu / g;

[0011] The effective live bacteria count of Ruminococcus xanthus in the Ruminococcus xanthus powder is 1.8 to 2.2×10 10 cfu / g;

[0012] The effective viable bacteria count of Bacillus subtilis in the Bacillus subtilis powder is 4.5 to 5.5×10 10 cfu / g;

[0013] The effective viable bacteria count of Acetobacter spp. in the Acetobacter spp. powder is 1.7 to 2.1×10 10 cfu / g;

[0014] The effective viable bacteria count of Lactobacillus plantarum in the Lactobacillus plantarum powder is 4 to 6×10 10 cfu / g.

[0015] The present invention also provides application of the bacterial agent in preparing a product for reducing methane emission of ruminants.

[0016] Preferably, the product is feed and the ruminant is cattle.

[0017] The present invention also provides a feed for reducing methane emissions from ruminants, the feed comprising the following raw materials in parts by weight: 1.5-2.5 parts of bacterial agent, 40-55 parts of silage corn, 10-15 parts of alfalfa, 10-15 parts of peanut vines, 4-6 parts of bean curd dregs, 1.3-1.7 parts of lauric acid, and 1-2 parts of flax seeds.

[0018] The present invention also provides a method for preparing the feed, comprising the following steps:

[0019] (1) grinding alfalfa and peanut vines to obtain a grinding material;

[0020] (2) mixing the crushed material, corn silage, bean curd residue, flax seeds and bacterial agent to obtain a mixed material;

[0021] (3) The mixed material is piled up and mixed with lauric acid, and dried to obtain the feed.

[0022] Preferably, the length of the crushed material in step (1) is ≤2 cm.

[0023] Preferably, the stacking temperature in step (3) is 25-35° C., the stacking time is 1-3 days, and the moisture content of the dried feed is ≤12%.

[0024] The present invention provides a bacterial agent for reducing methane emissions from ruminants, a preparation method thereof, and a compound feed containing the bacterial agent. The bacterial agent comprises the following raw materials in parts by weight: 6 to 10 parts of Bacteroides fragilis powder, 3 to 5 parts of white Ruminococcus powder, 3 to 5 parts of yellow Ruminococcus powder, 4 to 6 parts of Bacillus subtilis powder, 2 to 4 parts of Acetobacter striata powder, and 10 to 12 parts of Lactobacillus plantarum powder. The bacterial agent of the present invention can supplement beneficial microorganisms in the stomach of ruminants, increase the number of Bacteroides fragilis and white Ruminococcus, affect the concentration of metabolites myristic acid and palmitic acid, and thus reduce methane emissions. The prepared feed adjusts the nutritional structure of the diet, can reduce the fermentation of sugars in the rumen, inhibit the methane production pathway, and achieve synergistic effects with the bacterial agent. DETAILED DESCRIPTION

[0025] The invention provides a bacterial agent for reducing methane emissions from ruminants. The bacterial agent comprises the following raw materials in parts by weight: 6 to 10 parts of Bacteroides fragilis powder, 3 to 5 parts of white Ruminococcus powder, 3 to 5 parts of yellow Ruminococcus powder, 4 to 6 parts of Bacillus subtilis powder, 2 to 4 parts of Acetobacter striata powder and 10 to 12 parts of Lactobacillus plantarum powder.

[0026] In the present invention, the bacterial agent preferably includes the following raw materials in parts by weight: 7 to 9 parts of Bacteroides fragilis powder, 3.5 to 4.5 parts of white Ruminococcus powder, 3.5 to 4.5 parts of yellow Ruminococcus powder, 4.5 to 5.5 parts of Bacillus subtilis powder, 2.5 to 3.5 parts of Acetobacter striata powder and 10.5 to 11.5 parts of Lactobacillus plantarum powder.

[0027] In the present invention, the bacterial agent preferably includes the following raw materials in parts by weight: 8 parts of Bacteroides fragilis powder, 4 parts of white Ruminococcus powder, 4 parts of yellow Ruminococcus powder, 5 parts of Bacillus subtilis powder, 3 parts of Acetobacter membranaceus powder and 10 parts of Lactobacillus plantarum powder.

[0028] In the present invention, the effective viable count of Bacteroides fragilis in the Bacteroides fragilis powder is preferably 1.2 to 1.8×10 10 cfu / g, more preferably 1.5×10 10 cfu / g;

[0029] The effective viable bacteria count of the white rumenococcus in the white rumenococcus powder is preferably 3.0 to 3.6×10 10 cfu / g, more preferably 3.3×10 10 cfu / g;

[0030] The effective viable bacteria count of Ruminococcus xanthus in the Ruminococcus xanthus powder is preferably 1.8 to 2.2×10 10 cfu / g, more preferably 2×10 10 cfu / g;

[0031] The effective viable bacteria count of Bacillus subtilis in the Bacillus subtilis powder is preferably 4.5 to 5.5×10 10 cfu / g, more preferably 5×10 10 cfu / g;

[0032] The effective viable bacteria count of Acetobacter spp. in the Acetobacter spp. powder is preferably 1.7 to 2.1×10 10 cfu / g, more preferably 1.9×10 10 cfu / g;

[0033] The effective viable number of Lactobacillus plantarum in the Lactobacillus plantarum powder is preferably 4 to 6×10 10 cfu / g, more preferably 5×10 10 cfu / g.

[0034] The present invention also provides application of the bacterial agent in preparing a product for reducing methane emission of ruminants.

[0035] In the present invention, the type of the product is preferably feed, and the ruminant is preferably cattle.

[0036] The present invention also provides a feed for reducing methane emissions from ruminants, the feed comprising the following raw materials in parts by weight: 1.5-2.5 parts of bacterial agent, 40-55 parts of silage corn, 10-15 parts of alfalfa, 10-15 parts of peanut vines, 4-6 parts of bean curd dregs, 1.3-1.7 parts of lauric acid, and 1-2 parts of flax seeds.

[0037] In the present invention, the amount of the bacterial agent is preferably 1.8 to 2.2 parts, more preferably 2 parts.

[0038] In the present invention, the number of portions of the silage corn is preferably 45 to 50 portions, and more preferably 47 to 48 portions.

[0039] In the present invention, the amount of alfalfa is preferably 11 to 14 parts, and more preferably 12 to 13 parts.

[0040] In the present invention, the number of portions of the peanut vines is preferably 11 to 14 portions, and more preferably 12 to 13 portions.

[0041] In the present invention, the amount of the tofu dregs is preferably 4.5 to 5.5 parts, more preferably 5 parts.

[0042] In the present invention, the amount of lauric acid is preferably 1.4 to 1.6 parts, more preferably 1.5 parts.

[0043] In the present invention, the amount of flaxseed is preferably 1.2 to 1.8 parts, more preferably 1.5 parts.

[0044] The present invention also provides a method for preparing the feed, comprising the following steps:

[0045] (1) grinding alfalfa and peanut vines to obtain a grinding material;

[0046] (2) mixing the crushed material, corn silage, bean curd residue, flax seeds and bacterial agent to obtain a mixed material;

[0047] (3) The mixed material is piled up and mixed with lauric acid, and dried to obtain the feed.

[0048] In the present invention, the length of the crushed material in step (1) is preferably ≤2 cm, and more preferably ≤1.5 cm.

[0049] In the present invention, the stacking temperature in step (3) is preferably 25-35°C, more preferably 30°C, the stacking time is preferably 1-3 days, more preferably 2 days, and the moisture content of the dried feed is preferably ≤12%, more preferably ≤10%.

[0050] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0051] Example 1

[0052] A method for preparing feed for reducing methane emissions from ruminants, comprising the following steps:

[0053] (1) weighing raw materials according to the mass ratio of 6 parts of Bacteroides fragilis powder, 5 parts of white Ruminococcus powder, 5 parts of yellow Ruminococcus powder, 4 parts of Bacillus subtilis powder, 2 parts of Acetobacter membranaceus powder and 10 parts of Lactobacillus plantarum powder, and mixing them to obtain a microbial agent;

[0054] (2) weighing raw materials according to the mass ratio of 2.5 parts of microbial agent, 40 parts of silage corn, 15 parts of alfalfa, 15 parts of peanut seedlings, 4 parts of tofu residue, 1.7 parts of lauric acid, and 2 parts of flaxseed, and set aside;

[0055] (3) crushing alfalfa and peanut vines to a length of ≤2 cm to obtain crushed material;

[0056] (4) mixing the crushed material, corn silage, bean curd residue, flax seeds and microbial agent to obtain a mixed material;

[0057] (5) The mixed material is piled at 25° C. for 3 days, mixed with lauric acid, and dried to a water content of ≤12% to obtain the feed.

[0058] Note: In this embodiment

[0059] The effective viable count of Bacteroides fragilis in Bacteroides fragilis powder was 1.2×10 10 cfu / g;

[0060] The effective viable count of white Ruminococcus in white Ruminococcus powder was 3.6×10 10 cfu / g;

[0061] The effective viable bacteria count of Ruminococcus luteus in the powder of Ruminococcus luteus was 2.2×10 10 cfu / g;

[0062] The effective viable count of Bacillus subtilis in Bacillus subtilis powder is 4.5×10 10 cfu / g;

[0063] The effective viable count of Acetobacter spp. in Acetobacter spp. powder was 2.1×10 10 cfu / g;

[0064] The effective viable count of Lactobacillus plantarum in Lactobacillus plantarum powder is 4×10 10 cfu / g.

[0065] Example 2

[0066] A method for preparing feed for reducing methane emissions from ruminants, comprising the following steps:

[0067] (1) weighing raw materials according to the mass ratio of 10 parts of Bacteroides fragilis powder, 3 parts of white Ruminococcus powder, 3 parts of yellow Ruminococcus powder, 6 parts of Bacillus subtilis powder, 4 parts of Acetobacter membranaceus powder and 12 parts of Lactobacillus plantarum powder, and mixing them to obtain a microbial agent;

[0068] (2) weighing raw materials according to the mass ratio of 1.5 parts of microbial agent, 55 parts of silage corn, 10 parts of alfalfa, 10 parts of peanut seedlings, 6 parts of tofu residue, 1.3 parts of lauric acid, and 1 part of flaxseed, and set aside;

[0069] (3) crushing alfalfa and peanut vines to a length of ≤2 cm to obtain crushed material;

[0070] (4) mixing the crushed material, corn silage, bean curd residue, flax seeds and microbial agent to obtain a mixed material;

[0071] (5) The mixed material is piled at 35° C. for 1 day, mixed with lauric acid, and dried to a water content of ≤12% to obtain the feed.

[0072] Note: In this embodiment

[0073] The effective viable count of Bacteroides fragilis in Bacteroides fragilis powder was 1.8×10 10 cfu / g;

[0074] The effective viable count of white Ruminococcus in the white Ruminococcus powder was 3.0×10 10 cfu / g;

[0075] The effective viable count of Ruminococcus xanthus in the powder was 1.8×10 10 cfu / g;

[0076] The effective viable count of Bacillus subtilis in Bacillus subtilis powder is 5.5×10 10 cfu / g;

[0077] The effective viable count of Acetobacter spp. in Acetobacter spp. powder was 1.7×10 10 cfu / g;

[0078] The effective viable count of Lactobacillus plantarum in Lactobacillus plantarum powder is 6×10 10 cfu / g.

[0079] Example 3

[0080] A method for preparing feed for reducing methane emissions from ruminants, comprising the following steps:

[0081] (1) weighing raw materials according to the mass ratio of 8 parts of Bacteroides fragilis powder, 4 parts of white Ruminococcus powder, 4 parts of yellow Ruminococcus powder, 5 parts of Bacillus subtilis powder, 3 parts of Acetobacter membranaceus powder and 11 parts of Lactobacillus plantarum powder, and mixing them to obtain a microbial agent;

[0082] (2) weighing raw materials according to the mass ratio of 2 parts of microbial agent, 45 parts of silage corn, 13 parts of alfalfa, 13 parts of peanut seedlings, 5 parts of tofu residue, 1.5 parts of lauric acid, and 1.5 parts of flaxseed, and set aside;

[0083] (3) crushing alfalfa and peanut vines to a length of ≤2 cm to obtain crushed material;

[0084] (4) mixing the crushed material, corn silage, bean curd residue, flax seeds and microbial agent to obtain a mixed material;

[0085] (5) The mixed material is piled at 30° C. for 2 days, mixed with lauric acid, and dried to a water content of ≤12% to obtain the feed.

[0086] Note: In this embodiment

[0087] The effective viable count of Bacteroides fragilis in Bacteroides fragilis powder was 1.5×10 10 cfu / g;

[0088] The effective viable count of white Ruminococcus in the white Ruminococcus powder was 3.3×10 10 cfu / g;

[0089] The effective viable count of Ruminococcus flavoscens in the powder is 2×10 10 cfu / g;

[0090] The effective viable count of Bacillus subtilis in Bacillus subtilis powder is 5×10 10 cfu / g;

[0091] The effective viable count of Acetobacter spp. in Acetobacter spp. powder was 1.9×10 10 cfu / g;

[0092] The effective viable count of Lactobacillus plantarum in Lactobacillus plantarum powder is 5×10 10 cfu / g.

[0093] Comparative Example 1

[0094] A method for preparing feed for reducing methane emissions from ruminants, comprising the following steps:

[0095] (1) Weigh raw materials according to the mass ratio of 45 parts of silage corn, 13 parts of alfalfa, 13 parts of peanut vines, 5 parts of tofu residue, 1.5 parts of lauric acid, and 1.5 parts of flaxseed, and set aside;

[0096] (2) crushing alfalfa and peanut vines to a length of ≤2 cm to obtain crushed material;

[0097] (3) mixing the crushed material, corn silage, bean curd residue and flax seeds to obtain a mixed material;

[0098] (4) The mixed material is piled at 30° C. for 2 days, mixed with lauric acid, and dried to a water content of ≤12% to obtain the feed.

[0099] Comparative Example 2

[0100] A method for preparing feed for reducing methane emissions from ruminants, comprising the following steps:

[0101] (1) weighing raw materials according to the mass ratio of 8 parts of Bacteroides fragilis powder, 4 parts of white Ruminococcus powder, 4 parts of yellow Ruminococcus powder, 5 parts of Bacillus subtilis powder, 3 parts of Acetobacter membranaceus powder and 11 parts of Lactobacillus plantarum powder, and mixing them to obtain a microbial agent;

[0102] (2) Weigh the raw materials according to the mass ratio of 2 parts of microbial agent to 79 parts of conventional diet and set aside;

[0103] (3) mixing the conventional diet and the microbial agent to obtain a mixed feed;

[0104] (5) The mixed material is piled at 30° C. for 2 days and dried to a moisture content of ≤12% to obtain the feed.

[0105] Note: In this embodiment

[0106] The effective viable count of Bacteroides fragilis in Bacteroides fragilis powder was 1.5×10 10 cfu / g;

[0107] The effective viable count of white Ruminococcus in the white Ruminococcus powder was 3.3×10 10 cfu / g;

[0108] The effective viable count of Ruminococcus flavoscens in the powder is 2×10 10 cfu / g;

[0109] The effective viable count of Bacillus subtilis in Bacillus subtilis powder is 5×10 10 cfu / g;

[0110] The effective viable count of Acetobacter spp. in Acetobacter spp. powder was 1.9×10 10 cfu / g;

[0111] The effective viable count of Lactobacillus plantarum in Lactobacillus plantarum powder is 5×10 10 cfu / g.

[0112] The formula of the conventional diet (by weight) is 60 parts of sheep fescue, 27.16 parts of corn, 4.43 parts of soybean meal, 2.46 parts of rice bran meal, 3.15 parts of DDGS, 0.2 parts of stone powder, 0.3 parts of salt, 0.3 parts of sodium bicarbonate, and 2 parts of premix; the premix provides per kilogram of feed: vitamin A 2500IU, vitamin D 650IU, vitamin E 25mg, iron 42mg, manganese 30mg, copper 20mg, zinc 45mg, cobalt 0.3mg, selenium 0.6mg, and iodine 0.8mg.

[0113] Test example

[0114] Eighteen Chinese Grassland Red Cattle aged 18 months were selected and equally divided into 6 groups with 3 cattle in each group, numbered as experimental groups 1-3, control groups 1-2 and a blank control group.

[0115] The cattle in the test group 1 were fed with the feed prepared in Example 1, the cattle in the test group 2 were fed with the feed prepared in Example 2, the cattle in the test group 3 were fed with the feed prepared in Example 3, the cattle in the control group 1 were fed with the feed prepared in Comparative Example 1, the cattle in the control group 2 were fed with the feed prepared in Comparative Example 2, and the cattle in the blank control group were fed with conventional diets.

[0116] The formula of the conventional diet (by weight) is 60 parts of leymus chinensis, 27.16 parts of corn, 4.43 parts of soybean meal, 2.46 parts of rice bran meal, 3.15 parts of DDGS, 0.2 parts of stone powder, 0.3 parts of salt, 0.3 parts of sodium bicarbonate, and 2 parts of premix;

[0117] The premix provides per kilogram of feed: vitamin A 2500IU, vitamin D 650IU, vitamin E 25mg, iron 42mg, manganese 30mg, copper 20mg, zinc 45mg, cobalt 0.3mg, selenium 0.6mg, and iodine 0.8mg.

[0118] The experiment was conducted under the same feeding conditions, with free access to food and water during the experiment. The experiment lasted for 10 days. Respiration was monitored using a large animal respiratory calorimeter. The average gas emissions of each group of cattle were recorded on the 7th and 10th days after the start of the experiment. The results are shown in Table 1.

[0119] Table 1

[0120] Group Methane emissions on the 7th day (L / d) Methane emissions on the 10th day (L / d) Experimental Group 1 207.91 201.23 Experimental Group 2 208.50 203.47 Experimental Group 3 203.67 198.68 Control group 1 235.11 234.18 Control group 2 219.31 215.81 Blank control group 237.91 238.15

[0121] Conclusion: The bacterial agent of the present invention can supplement the beneficial microorganisms in the stomach of ruminants. Combined with a special diet, it can reduce the fermentation of sugars in the rumen, inhibit the methane production pathway, achieve a synergistic effect, and jointly reduce the methane emissions of ruminants.

[0122] As can be seen from the above embodiments, the present invention provides a bacterial agent for reducing methane emissions from ruminants, a preparation method thereof, and a compound feed containing the bacterial agent, wherein the bacterial agent comprises the following raw materials in parts by weight: 6 to 10 parts of Bacteroides fragilis powder, 3 to 5 parts of white Ruminococcus powder, 3 to 5 parts of yellow Ruminococcus powder, 4 to 6 parts of Bacillus subtilis powder, 2 to 4 parts of Acetobacter striata powder, and 10 to 12 parts of Lactobacillus plantarum powder. The bacterial agent of the present invention can supplement the beneficial microorganisms in the stomach of ruminants, increase the number of Bacteroides fragilis and white Ruminococcus, affect the concentration of metabolites myristic acid and palmitic acid, and thus reduce methane emissions. The prepared feed adjusts the nutritional structure of the diet, can reduce the fermentation of sugars in the rumen, inhibit the methane production pathway, and achieve synergistic effects with the bacterial agent.

[0123] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A bacterial agent for reducing methane emissions from ruminants, characterized in that: The bacterial agent is composed of the following raw materials in parts by weight: 6-10 parts of Bacteroides fragilis powder, 3-5 parts of white Ruminococcus powder, 3-5 parts of yellow Ruminococcus powder, 4-6 parts of Bacillus subtilis powder, 2-4 parts of Acetobacter striata powder and 10-12 parts of Lactobacillus plantarum powder; The effective viable bacteria count of Bacteroides fragilis in the Bacteroides fragilis powder is 1.2 to 1.8×10 10 cfu / g; The effective viable bacteria count of the white rumenococcus in the white rumenococcus powder is 3.0 to 3.6×10 10 cfu / g; The effective live bacteria count of the Ruminococcus xanthus in the Ruminococcus xanthus powder is 1.8 to 2.2×10 10 cfu / g; The effective viable bacteria count of Bacillus subtilis in the Bacillus subtilis powder is 4.5 to 5.5×10 10 cfu / g; The effective viable bacteria count of Acetobacter spp. in the Acetobacter spp. powder is 1.7 to 2.1×10 10 cfu / g; The effective viable bacteria count of Lactobacillus plantarum in the Lactobacillus plantarum powder is 4 to 6×10 10 cfu / g.

2. The bacterial agent according to claim 1, characterized in that The bacterial agent is composed of the following raw materials in parts by weight: 7-9 parts of Bacteroides fragilis powder, 3.5-4.5 parts of white Ruminococcus powder, 3.5-4.5 parts of yellow Ruminococcus powder, 4.5-5.5 parts of Bacillus subtilis powder, 2.5-3.5 parts of Acetobacter striata powder and 10.5-11.5 parts of Lactobacillus plantarum powder.

3. The bacterial agent according to claim 2, characterized in that The bacterial agent is composed of the following raw materials in parts by weight: 8 parts of Bacteroides fragilis powder, 4 parts of white Ruminococcus powder, 4 parts of yellow Ruminococcus powder, 5 parts of Bacillus subtilis powder, 3 parts of Acetobacter membranaceus powder and 10 parts of Lactobacillus plantarum powder.

4. Use of the bacterial agent according to any one of claims 1 to 3 in the preparation of a product for reducing methane emissions from ruminants.

5. The use according to claim 4, characterized in that: The product is feed and the ruminant is cattle.

6. A feed for reducing methane emissions from ruminants, characterized in that: The feed comprises the following raw materials in parts by weight: 1.5-2.5 parts of the bacterial agent according to any one of claims 1 to 3, 40-55 parts of silage corn, 10-15 parts of alfalfa, 10-15 parts of peanut seedlings, 4-6 parts of bean curd residue, 1.3-1.7 parts of lauric acid, and 1-2 parts of flaxseed; The method for preparing the feed comprises the following steps: (1) grinding alfalfa and peanut vines to obtain a grinding material; (2) mixing crushed material, corn silage, bean curd residue, flax seeds and the bacterial agent according to any one of claims 1 to 4 to obtain a mixed material; (3) the mixed material is piled up, mixed with lauric acid, and dried to obtain the feed; The stacking temperature in step (3) is 25-35° C., the stacking time is 1-3 days, and the moisture content of the dried feed is ≤12%.

7. The method for preparing the feed according to claim 6, characterized in that: The steps include: (1) grinding alfalfa and peanut vines to obtain a grinding material; (2) mixing crushed material, corn silage, bean curd residue, flax seeds and the bacterial agent according to any one of claims 1 to 4 to obtain a mixed material; (3) the mixed material is piled up, mixed with lauric acid, and dried to obtain the feed; The stacking temperature in step (3) is 25-35° C., the stacking time is 1-3 days, and the moisture content of the dried feed is ≤12%.

8. The preparation method according to claim 7, characterized in that: The length of the crushed material in step (1) is ≤2 cm.