Biological organic conditioner for saline-alkali land and its preparation method and application

Abstract

The present invention provides a biological organic conditioner for saline-alkali land, a preparation method and an application thereof. The biological organic conditioner comprises 50-60 parts of sheep manure organic fertilizer, 10-20 parts of humic acid, 5-10 parts of arbuscular mycorrhizal fungi agent, 5-10 parts of potassium sulfate, 15-20 parts of urea, 5-10 parts of calcium ammonium nitrate, and 20-30 parts of liquid binder. The provided biological organic conditioner can be used for saline-alkali land soil to improve soil fertility and increase crop yield.

Description

Technical Field

[0001] The invention belongs to the technical field of soil saline-alkali land improvement, and in particular relates to a biological organic conditioner for saline-alkali land, a preparation method and an application thereof. Background Art

[0002] Salt-alkali land is a progressive soil degradation problem. Its poor soil physical and chemical properties seriously endanger soil biological activity and crop yields, and undermine regional agricultural development and ecological civilization construction. However, after proper restoration and improvement, salt-alkali land can be used as an important reserve arable land resource to ensure food security. At present, the development and utilization of salt-alkali land resources have relatively complete technical improvement means, through physical methods such as underground pipe salt discharge and guest sand replacement, chemical methods such as application of phosphogypsum and biochar, and biological control measures such as planting salt-alkali tolerant plants such as alfalfa and sheepgrass. Among them, chemical improvement methods need to pay special attention to the dosage problem. Excessive application of chemical improvers is not only easy to cause problems such as extremely high improvement costs, but also easy to cause secondary soil pollution. In addition, biological control measures for saline-alkali land still rely on the foundation of previous soil improvement. Therefore, saline-alkali barrier soil remediation is the key to the utilization of saline-alkali land resources. However, the current development and utilization of saline-alkali land still generally has problems such as low soil nutrient content and insufficient fertility, which need further improvement. Summary of the invention

[0003] The present invention aims to solve at least one of the technical problems in the related art to a certain extent. The present invention aims to solve the practical problems of poor soil fertility and nutrient deficiency commonly found in saline-alkali land, and provides a biological organic conditioner for saline-alkali land, a preparation method and an application thereof. The provided biological organic conditioner can be particularly used in the field of saline-alkali land soil, for improving saline-alkali land soil, improving soil fertility and increasing crop yield.

[0004] Specifically, the present invention provides the following technical solutions:

[0005] The first aspect of the present invention provides a biological organic conditioner for saline-alkali land, comprising:

[0006] 50-60 parts of sheep manure organic fertilizer, 10-20 parts of humic acid, 5-10 parts of arbuscular mycorrhizal fungi agent, 5-10 parts of potassium sulfate, 15-20 parts of urea, 5-10 parts of calcium ammonium nitrate, and 20-30 parts of liquid binder.

[0007] According to an embodiment of the present invention, the above-mentioned biological organic conditioner for saline-alkali land may further include the following technical features:

[0008] According to an embodiment of the present invention, the arbuscular mycorrhizal fungal agent is an intraradical rhizosporoides fungal agent.

[0009] According to an embodiment of the present invention, the intraradical rhizosporon fungus is Glomus intraradical HDSF5, which is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration with a number of CGMCC NO.10607.

[0010] According to an embodiment of the present invention, the organic matter content of the sheep manure organic fertilizer is ≥28%, the total nitrogen content is ≥0.8%, and the total potassium content is ≥1.0%.

[0011] According to an embodiment of the present invention, the liquid binder is sterilized water.

[0012] The second aspect of the present invention provides a method for preparing a bio-organic conditioning agent, wherein the bio-organic conditioning agent is the bio-organic conditioning agent described in the first aspect, and the preparation method comprises the following steps:

[0013] (1) mixing the sheep manure organic fertilizer, humic acid and arbuscular mycorrhizal fungi agent uniformly, grinding and sieving to obtain a primary dry material mixture;

[0014] (2) granulating the primary dry material mixture and the liquid binder, and drying after forming to obtain a primary granular material;

[0015] (3) adding the primary granular material to a mixture of potassium sulfate, urea and calcium ammonium nitrate to obtain a secondary material mixture;

[0016] (4) pouring the secondary material mixture into a coating machine, adding a liquid binder, so that the potassium sulfate, urea, and calcium ammonium nitrate mixture in the secondary material mixture is uniformly wrapped and adsorbed on the surface of the primary granular material to form a coating, granulating, and drying after forming, so as to obtain the biological organic conditioner.

[0017] According to an embodiment of the present invention, the drying temperature in step (2) and step (4) is 30-50°C.

[0018] According to an embodiment of the present invention, the moisture content of the bio-organic conditioning agent is ≤10 wt %.

[0019] The third aspect of the present invention provides an application of the biological organic conditioner described in any embodiment of the first aspect in the field of soil saline-alkali land improvement.

[0020] A fourth aspect of the present invention provides a method for fertilizing saline-alkali soil, comprising: applying the biological organic conditioner described in any embodiment of the first aspect to the saline-alkali soil.

[0021] The beneficial effects achieved by the present invention are:

[0022] (1) Supplementing nutrients lacking in the soil. The biological organic conditioner provided by the present invention is rich in mineral elements such as C, N, P, K and organic matter sheep manure organic fertilizer, which can improve soil nutrient conditions and improve the crop growth environment in saline-alkali land with poor soil fertility.

[0023] (2) Preserve soil nutrients. The provided biological organic conditioner contains arbuscular mycorrhizal fungi, the hyphae of which can provide a place and system for plant roots and microorganisms to absorb and retain nutrients, making it difficult for nutrients added to saline-alkali soil to be leached with subsequent tillage and irrigation measures.

[0024] (3) Improve the soil structure of saline-alkali land. Saline-alkali land generally has problems such as dispersed soil particles and poor water permeability and aeration due to compacted structure. The provided biological organic conditioner with coexistence of organic fertilizer and mycorrhizal fungi has a low bulk density and a loose structure. The dense hyphae of arbuscular mycorrhizal fungi can cross-link soil particles and improve the soil's resistance to compaction. Application to the soil can reduce the bulk density of saline-alkali soil and improve the problems of soil compaction and poor water permeability and aeration. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a diagram showing the change in corn plant height after applying a saline-alkali soil biological organic conditioner according to an embodiment of the present invention.

[0026] Figure 2 This is a graph showing changes in soil organic matter content in the surface layer 0-20 cm after application of a saline-alkali land biological organic conditioner according to an embodiment of the present invention.

[0027] Figure 3 This is a graph showing changes in soil salt content in the surface layer 0-20 cm after application of a saline-alkali land biological organic conditioner according to an embodiment of the present invention. DETAILED DESCRIPTION

[0028] Embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but should not be construed as limiting the present invention.

[0029] The present invention provides a biological organic conditioner for saline-alkali land, comprising:

[0030] 50-60 parts of sheep manure organic fertilizer, 10-20 parts of humic acid, 5-10 parts of arbuscular mycorrhizal fungi agent, 5-10 parts of potassium sulfate, 15-20 parts of urea, 5-10 parts of calcium ammonium nitrate, and 20-30 parts of liquid adhesive.

[0031] The biological organic conditioner provided by the present invention aims to improve the fertility of saline-alkali land and promote crop yield. During the research process, it was found that if certain ingredients were not added or added in small amounts, such as if arbuscular mycorrhizal fungi agents were not added, the root length, plant height and biomass of the crop were reduced, which ultimately led to a decrease in yield; for another example, when the amount of humic acid added was small (for example, only 5 parts), it was found that the chlorophyll content, plant height and yield of the crop were reduced.

[0032] According to a specific embodiment, the provided biological organic conditioner consists of only these ingredients, namely: 50-60 parts of sheep manure organic fertilizer, 10-20 parts of humic acid, 5-10 parts of arbuscular mycorrhizal fungi agent, 5-10 parts of potassium sulfate, 15-20 parts of urea, 5-10 parts of calcium ammonium nitrate, and 20-30 parts of liquid adhesive. According to a preferred embodiment, the provided biological organic conditioner includes: 53-58 parts of sheep manure organic fertilizer, 13-18 parts of humic acid, 8-10 parts of arbuscular mycorrhizal fungi agent, 8-10 parts of potassium sulfate, 15-20 parts of urea, 8-10 parts of calcium ammonium nitrate, and 22-28 parts of liquid adhesive. The sheep manure organic fertilizer used has a high organic matter content and contains an appropriate amount of nitrogen and potassium. According to a specific embodiment, the organic matter content in the sheep manure organic fertilizer is ≥28%, the total nitrogen content is ≥0.8%, and the total potassium content is ≥1.0%.

[0033] Arbuscular mycorrhizal fungi (AMF) used is a kind of plant symbiotic fungi, which forms mycorrhizal symbionts by symbiosis with plant roots. Its widely distributed hyphae and secreted glomalin-related proteins can improve the adaptability of plants to adversity and promote soil fertilization. Organic fertilizer, as a kind of green conditioning material, can not only supplement soil organic matter and quick-acting nutrient content through the companion products of animal husbandry industry, but also improve soil physical structure, make soil texture more permeable, easy to discharge salt and retain moisture. At the same time, humic acid can improve soil porosity and promote aggregate formation in physical aspect, and promote organic matter decomposition and enzyme activity in biochemistry. Through these component compounding, the biological organic conditioning agent coordinated by microbial preparation and organic fertilizer can promote the improvement of crop production capacity on the basis of improving soil fertility. According to a specific embodiment, the arbuscular mycorrhizal fungi agent is a root intraradical cyst mold agent.

[0034] According to a specific implementation method, the root spore mold is Glomus radicis HDSF5, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Committee on March 11, 2015, and the deposit number is CGMCC No. 10607. Glomus radicis HDSF can grow in a poor saline-alkali soil environment, with an optimal growth temperature of 18 to 25 degrees Celsius and an optimal pH of 6.5 to 7.5. The microorganism is recorded in a Chinese patent application with application number 201510232876.3 and authorization announcement number CN104805020B. The corresponding microbial agent can be prepared by the following method:

[0035] After obtaining the Glomus intraradices HDSF5 strain from the General Microbiology Center of China National Microbiological Culture Collection Committee, it was propagated in a culture solution to obtain a mixture containing the propagated strain, which was freeze-dried to obtain fungal powder, namely the arbuscular mycorrhizal fungal agent.

[0036] According to a specific embodiment, the liquid binder is sterile water, and the sterile water mentioned refers to deionized water sterilized at high temperature.

[0037] The present invention also provides a method for preparing the above-mentioned biological organic conditioning agent, comprising:

[0038] (1) mixing the sheep manure organic fertilizer, humic acid and arbuscular mycorrhizal fungi agent uniformly, grinding and sieving to obtain a primary dry material mixture;

[0039] (2) granulating the primary dry material mixture and the liquid binder, and drying after forming to obtain a primary granular material;

[0040] (3) adding the primary granular material to a mixture of potassium sulfate, urea and calcium ammonium nitrate to obtain a secondary material mixture;

[0041] (4) pouring the secondary material mixture into a coating machine, adding a liquid binder, so that the potassium sulfate, urea, and calcium ammonium nitrate mixture in the secondary material mixture is evenly wrapped and adsorbed on the surface of the primary granular material to form a coating, granulating, and drying after forming to obtain a biological organic conditioner.

[0042] According to a specific implementation manner, in step (1), the sheep manure organic fertilizer, humic acid and arbuscular mycorrhizal fungi agent are mixed, stirred evenly, ground and sieved to obtain a primary dry material mixture.

[0043] According to a specific embodiment, in step (2), the primary dry material mixture obtained in step (1) is put into a granulator for granulation. During the granulation process, a liquid binder is sprayed into the primary dry material mixture to form granules. After granulation, the granular material is dried and cooled to obtain a primary granular material.

[0044] According to a specific implementation paradigm, in step (3), potassium sulfate, urea and calcium ammonium nitrate are mixed evenly, and then the primary granular material prepared in step (2) is put into the above three material mixture and stirred and mixed evenly to obtain a secondary material mixture.

[0045] According to a specific embodiment, the primary dry material mixture obtained in step (2) and the secondary material mixture in step (3) are placed in a coating machine. During the coating process, a liquid binder is sprayed into the secondary material mixture, and the coating and granulation are performed. After granulation, the granular material is dried and cooled to prepare a bio-organic conditioning agent.

[0046] According to a specific embodiment, the drying temperature during the granulation process in step (2) and step (4) is 30-50°C, for example, 30°C, 35°C, 40°C, 45°C or 50°C.

[0047] After granulation, the moisture content of the bio-organic conditioning agent particles prepared above is ≤10wt%.

[0048] The provided bio-organic conditioning agent can be applied by referring to the following method: during spring ploughing, the bio-organic conditioning agent is evenly spread on the soil surface one day before the rotary tillage of the farmland. The soil is then rotary tilled to rotate the bio-organic conditioning agent into the soil to mix it evenly with the soil. Watering is performed once 1-2 days before planting crops to promote the dissolution of the bio-organic conditioning agent and the activation of the microbial agent.

[0049] The soil rotary tillage is performed using a rotary tiller with 6 30 cm long spiral drill bits and a rotation speed of 3000 r / min, and the tillage depth is 20 cm below the soil surface. The surface soil and the bio-organic conditioning agent particles are fully mixed by the rotary tiller to facilitate nutrient storage and slow release.

[0050] The technical scheme of the present invention is described below by specific examples. It should be noted that these examples are only used to facilitate the understanding of those skilled in the art and should not be regarded as limiting the scope of protection of the present invention. The reagents used in the embodiments, unless otherwise specified, can all be obtained commercially.

[0051] Example 1

[0052] Ningxia Hetao Irrigation Area is one of the main saline soil distribution areas in my country. The soil used in this embodiment is located in the saline-alkali land distribution area of ​​Shizuishan City, Ningxia. The total salt content of the surface soil is 3.98g / kg, the pH is 8.63, and the soil texture is clay loam. The biological organic conditioner belonging to the present invention is the main research object, and the treatment without the conditioner is used as a control.

[0053] The specific formula of the biological organic conditioning agent used is as follows:

[0054] 58 parts of sheep manure organic fertilizer, 15 parts of humic acid, 8 parts of arbuscular mycorrhizal fungi agent, 6 parts of potassium sulfate, 180 parts of urea, 8 parts of calcium ammonium nitrate, and 25 parts of liquid binder; the arbuscular mycorrhizal fungi agent used is Glomus radicifolius HDSF5, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Committee and is numbered as CGMCC NO.10607; the liquid binder used is sterile water.

[0055] The bio-organic conditioning agent is prepared by the following method:

[0056] Step S1, mixing the sheep manure organic fertilizer, humic acid and arbuscular mycorrhizal fungi agent, stirring evenly, grinding and sieving to obtain a primary dry material mixture;

[0057] Step S2, putting the primary dry material mixture obtained in step S1 into a granulator for granulation, during which a liquid binder is sprayed into the primary dry material mixture for granulation. After granulation, the granular material is dried and cooled.

[0058] Step S3, mixing potassium sulfate, urea and calcium ammonium nitrate evenly, and then putting the granular material prepared in step S2 into the mixture of the above three materials and stirring and mixing them evenly.

[0059] Step S4, putting the mixture in step S3 into a granulator for secondary granulation, during which liquid binder is sprayed into the primary dry material mixture to form granules. After granulation, the granular material is dried and cooled to prepare a bio-organic conditioning agent.

[0060] The main implementation steps are as follows:

[0061] (1) Cultivated land: Before spring tillage, evenly spread the bio-organic conditioner on the soil surface, and then use a rotary tiller with 6 30 cm long spiral drills at a speed of 3000 r / min to rotate and till the tillage depth to 20 cm below the soil surface. The surface soil and the bio-organic conditioner particles are fully mixed by rotary tillage, and then the land is leveled.

[0062] (2) Sowing: corn seeds were sown in the soil after rotary tillage according to the conventional mechanized planting method, and the management during the crop growth period was carried out according to the conventional method. The sowing date, harvest date, sowing amount, base fertilizer application amount, topdressing application amount, etc. were all the same as conventional planting.

[0063] (3) Soil monitoring: soil samples are collected regularly during crop growth to monitor salinity data, and the changes in soil and crop indicators under the application of the bio-organic conditioner are compared with those of the control treatment.

[0064] The results showed that the soil salinity was lower after applying the bio-organic conditioner, and the nutrient indexes such as organic matter were significantly improved ( Figure 2 The plant height and dry weight of the crops treated with the bio-organic conditioning agent were higher than those of the control treatment ( Figure 1 ). It can be seen that the use of the biological organic conditioner provided by the present invention can significantly improve soil salinity, nutrients and crop growth indicators.

[0065] Example 2

[0066] The soil of Example 2 was collected from the saline-alkali land distribution area of ​​Bayannur City, Inner Mongolia. The surface soil conductivity can reach 14.86mS / cm, the pH reaches 9.59, and the soil texture is clay loam. The biological organic conditioner provided by the present invention is used as the main research object (the formula of the biological organic conditioner used is the same as that of Example 1), and the treatment without the conditioner is used as the control.

[0067] The main implementation steps are as follows:

[0068] (1) Cultivated land: Before spring tillage, evenly spread the bio-organic conditioner on the soil surface, and then use a rotary tiller with 6 30 cm long spiral drills at a speed of 3000 r / min to rotate and till the tillage depth to 20 cm below the soil surface. The surface soil and the bio-organic conditioner particles are fully mixed by rotary tillage, and then the land is leveled.

[0069] (2) Sowing: corn seeds were sown in conventional mechanized wide-narrow row method, and crop management during the growth period was carried out in accordance with conventional methods. The sowing date, harvest date, sowing amount, base fertilizer application amount, topdressing application amount, etc. were all consistent with the local management methods.

[0070] (3) Soil monitoring: soil samples are collected regularly during crop growth to monitor salinity data, and the changes in soil and crop indicators under the application of the bio-organic conditioner are compared with those of the control treatment.

[0071] The results showed that the application of the bio-organic conditioner significantly reduced soil salinity and significantly increased soil nutrient indicators such as organic matter ( Figure 2 , Figure 3 ). Among them, the plant height and chlorophyll index of the crops treated with the bio-organic conditioning agent were higher than those of the control treatment ( Figure 1 , Table 1). The application of the conditioning agent scheme of the present invention can significantly reduce soil salinity, increase soil nutrients, and improve crop growth indicators.

[0072] Table 1 Changes in corn dry weight and chlorophyll index after application of bio-organic conditioners

[0073] No additives added Bio-organic conditioning agent treatment Corn dry weight 116.00±3.61 165.67±5.86 Chlorophyll 50.33±1.53 54.33±2.08

[0074] Comparative Example 1

[0075] The difference between Comparative Example 1 and Example 1 is that no arbuscular mycorrhizal fungi agent is added to the specific components of the biological organic conditioning agent used in Comparative Example 1.

[0076] The results showed that the root length and plant height of corn in Comparative Example 1 were reduced by 20% and 23% respectively compared with those in Example 1, and the final yield was reduced by 15% compared with that in Example 1.

[0077] Comparative Example 2

[0078] The difference between Comparative Example 2 and Example 1 is that less humic acid is added to the specific components of the biological organic conditioning agent used in Comparative Example 2, and the content of the added humic acid is 5 parts.

[0079] The results showed that the chlorophyll content and plant height of corn leaves in Comparative Example 2 were respectively reduced by 11% and 8% compared with those in Example 1, and the final yield was reduced by 7% compared with that in Example 1.

[0080] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific implementation", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example.

[0081] The present invention is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principle of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A biological organic conditioner for saline-alkali land, It is characterized in that include: 50-60 parts of sheep manure organic fertilizer, 10-20 parts of humic acid, 5-10 parts of arbuscular mycorrhizal fungi agent, 5-10 parts of potassium sulfate, 15-20 parts of urea, 5-10 parts of calcium ammonium nitrate, and 20-30 parts of liquid binder; The arbuscular mycorrhizal fungal agent is an intraradical sporangiophore fungal agent; The root cyst fungus agent is the root cyst fungus HDSF5 agent, and the root cyst fungus HDSF5 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with the number CGMCC NO.10607.

2. The bio-organic conditioning agent according to claim 1, It is characterized in that The sheep manure organic fertilizer has an organic matter content of ≥28%, a total nitrogen content of ≥0.8%, and a total potassium content of ≥1.0%.

3. The bio-organic conditioning agent according to claim 1, It is characterized in that The liquid binder is sterilized water.

4. A method for preparing the bio-organic conditioning agent according to any one of claims 1 to 3, include: (1) mixing the sheep manure organic fertilizer, humic acid and arbuscular mycorrhizal fungi agent uniformly, grinding and sieving to obtain a primary dry material mixture; (2) granulating the primary dry material mixture and the liquid binder, and drying after forming to obtain a primary granular material; (3) adding the primary granular material to a mixture of potassium sulfate, urea and calcium ammonium nitrate to obtain a secondary material mixture; (4) pouring the secondary material mixture into a coating machine, adding a liquid binder, so that the mixture of potassium sulfate, urea and calcium ammonium nitrate in the secondary material mixture is uniformly wrapped and adsorbed on the surface of the primary granular material to form a coating, granulating, forming and drying, so as to obtain the bio-organic conditioning agent.

5. The preparation method according to claim 4, It is characterized in that The drying temperature in step (2) and step (4) is 30-50°C.

6. The preparation method according to claim 4, It is characterized in that The moisture content of the bio-organic conditioning agent is ≤10 wt %.

7. Use of the biological organic conditioner according to any one of claims 1 to 3 in the field of soil saline-alkali land improvement.

8. A method for fertilizing saline-alkali soil, It is characterized in that include: The bio-organic conditioner according to any one of claims 1 to 3 is applied to the saline-alkali soil.

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