Recombinant collagen Recomtein AAG-containing pure zinc oxide sunscreen lotion capable of resisting light aging and preparation method thereof

Abstract

The invention belongs to the field of cosmetics, and discloses a pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging and a preparation method thereof. The sunscreen lotion is prepared from a physical sunscreen agent, a light stabilizer, an emulsifier, an emollient, a dispersing agent, a thickening agent, a humectant, a preservative, an emulsion stabilizer, a chelating agent, a biological protection component and the balance of water, the physical sun-screening agent is zinc oxide subjected to surface treatment by triethoxyoctylsilane; the biological protection component is prepared from a highland barley fermentation liquid Supferm HIBY-PLUS (HydroxyIBY-Polylactic Acid) and a Recomtein AAG (Adenosine Adenosine Adenosine). The light stabilizer is added into the sunscreen cream, so that a relatively high sunscreen value is achieved by using less sunscreen agents, the amount of the sunscreen agents is less, correspondingly, heavy grease is also less, and the skin feeling is lighter and thinner.

Description

Technical Field

[0001] The present invention belongs to the field of cosmetics, and specifically relates to a cosmetic containing recombinant collagen AAG and pure zinc oxide sunscreen lotion capable of resisting photoaging and a preparation method thereof. Background Art

[0002] With the diversification of consumer demands, the trend of personalization and customization of sunscreen products is becoming more and more obvious. Consumers are no longer satisfied with general sunscreen products, but hope to find sunscreen products that suit them. At the same time, the market for skin care cosmetics is emerging, and consumers’ demand for sunscreen products is not only for high sun protection value and excellent refreshing skin texture, but also for multi-effect products. The damage of ultraviolet rays to the skin includes inflammation, oxidative stress, aging, darkening, etc. Consumers hope that sunscreen products can solve these problems and meet their needs for sun protection and anti-aging.

[0003] As consumers' awareness of skin protection increases, their requirements for the effectiveness and safety of sunscreen products increase, and the demand for high-end sunscreen products is also increasing. These products usually have higher SPF values, better waterproof performance, and more comfortable use experience.

[0004] For chemical sunscreens, small molecule sunscreens may have the risk of penetration, and large molecule sunscreens have limitations in application. At the same time, the sunscreen raw materials of most chemical sunscreens achieve the sunscreen effect through the principle of absorption. In view of the many shortcomings of chemical sunscreens. Physical sunscreens achieve the purpose of sunscreen by physically shielding, refracting and reflecting light. Its advantage is that the sunscreen is not absorbed by the skin, so it is highly safe and stable. It can be applied and sunscreened without waiting, and it can reflect ultraviolet rays for a long time. As long as you don't sweat or wipe, you can always maintain the sunscreen effect. And physical sunscreens do not react chemically on the skin, so they are relatively mild to the skin and suitable for a wide range of people. Summary of the invention

[0005] The present invention provides a pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging and a preparation method thereof. The present invention uses EWG green raw materials, is 3O-free, coral reef-friendly, OMC-free, octocrylene-free, and benzophenone-3-free; all ingredients are sensitive skin-friendly, and only the mineral inorganic sunscreen agent SolaPure ZnO C is added.

[0006] The technical solution of the present invention is as follows.

[0007] A pure zinc oxide sunscreen containing recombinant collagen Recomtein AAG and capable of resisting photoaging, comprising, by mass percentage, 20-30% of a physical sunscreen, 10-15% of a light stabilizer, 4-6% of an emulsifier, 20-25% of an emollient, 1-3% of a dispersant, 0.1-2% of a thickener, 8-10% of a moisturizer, 0.1-1% of a preservative, 0.1-2% of an emulsion stabilizer, 0.1-1% of a chelating agent and 10-15% of a biological protective component, with the remainder being water;

[0008] The physical sunscreen is zinc oxide surface-treated with triethoxyoctylsilane;

[0009] The biological protection components include highland barley fermentation liquid Supferm HIBY-PLUS and Recomtein AAG coagulogen.

[0010] Furthermore, the preparation method of the highland barley fermentation liquid Supferm HIBY-PLUS is as follows: prepare highland barley and water as fermentation substrates, the material-liquid ratio is: 1:200-1:100 g / mL, sterilize at high temperature and high pressure, cool down, and inoculate yeast; ferment, the fermentation temperature is: 27-30°C; after the fermentation is completed, remove impurities, take the filtrate, and obtain highland barley fermentation liquid.

[0011] Furthermore, the light stabilizer is one or more of acrylic acid (ester) copolymer or butyl octanol salicylate.

[0012] Furthermore, the emulsifier is one or more of lauryl PEG-9, polydimethylsiloxyethyl, polydimethylsiloxane, and cetyl PEG / PPG.

[0013] Furthermore, the emollient is one or more of cyclopentasiloxane or diphenylsiloxyphenyl trimethicone.

[0014] Furthermore, the dispersant is lauryl polyglyceryl-3 polydimethylsiloxyethyl polydimethylsiloxane.

[0015] Furthermore, the thickener is zinc stearate; and the humectant is one or more of butanediol and 1,2-hexanediol.

[0016] Furthermore, the preservative is p-hydroxyacetophenone; the emulsifier stabilizer is sodium chloride; and the chelating agent is EDTA-2NA.

[0017] A method for preparing a pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging comprises the following steps:

[0018] Step 1: After the light stabilizer, emulsifier and physical sunscreen are stirred, dispersed and moistened, the mixture is ground until uniformly dispersed to obtain component A;

[0019] Step 2: Heat and dissolve a light stabilizer, an emollient, and a thickener to obtain component B;

[0020] Step 3: Take an emulsifier and a light stabilizer and weigh them and mix them evenly to obtain component C; pour component A and component B into component C and place them in a water bath at 65-75°C to keep warm;

[0021] Step 4: Heat the solvent of xinxian ketone and 1,3-butanediol until it is transparent and free of particles, then add the chelating agent, sodium chloride, moisturizer, and deionized water, place in a 65-75°C water bath, stir and dissolve evenly until there are no particles, and then obtain component D;

[0022] Step 5: Mix Supferm HIBY-PLUS with AAG to obtain component E;

[0023] Step 6: Homogenize components A / B / C at 5000-7000 rpm for 5-8 min; then slowly pour component D into components A / B / C under stirring at 5000-7000 rpm for 5-8 min;

[0024] Step 7: After cooling to 40-50°C, add component E, and then cool to room temperature to obtain the target sunscreen product.

[0025] In the above method, the mass ratio of Supferm HIBY-PLUS to AAG is 10:1.

[0026] The raw materials of the present invention are described below.

[0027] Physical sunscreen: Solapure ZnO C

[0028] Sunscreen also belongs to the category of skin care. In the context of increasingly stringent market regulations and consumers' increasing demand for more natural products, the need to develop effective broad-spectrum sunscreens has attracted people's attention to zinc oxide. Hallstar provides SolaPure ZnO C, a zinc oxide surface-treated with triethoxycaprylylsilane that can provide convenience for formulation. It has excellent ability to protect against UVA and UVB while improving the transparency of the finished sunscreen product, with high transparency and good suspension and dispersibility. SolaPure ZnO C provides an essential solution for the development of safe, effective and elegant sunscreen products. The advantages of Solapure ZnO C products are extreme transparency, excellent dispersion, strong passivation and excellent performance; the real pure zinc oxide solution has better skin feel, more efficient photoactivity inhibition, better suspension and dispersion and better performance; pure zinc oxide is a physical sunscreen that can directly reflect and scatter ultraviolet rays to achieve sunscreen effect. Compared with chemical sunscreens, pure zinc oxide sunscreen is milder and less likely to cause allergic reactions.

[0029] Light Stabilizer:

[0030] Acrylic acid (ester) copolymer;

[0031] BHB: Butyloctanol Salicylate;

[0032] Emulsifier:

[0033] KF-6038: Lauryl PEG-9, Polydimethylsiloxyethyl, Polydimethylsiloxane;

[0034] KF-6048: Cetyl PEG / PPG;

[0035] Emollients:

[0036] KF-995: Cyclopentasiloxane;

[0037] KF-56A: Diphenylsiloxyphenyl trimethicone;

[0038] CRODAMOL AB-LQ-(RB): C12-15 alcohol benzoate;

[0039] Dispersant: KF-6115: Lauryl Polyglyceryl-3 Polydimethylsiloxyethyl Dimethicone;

[0040] Thickener: zinc stearate;

[0041] Humectants: Butylene glycol, 1,2-hexanediol;

[0042] Preservative: p-hydroxyacetophenone;

[0043] Emulsion stabilizer: sodium chloride;

[0044] Chelating agent: EDTA-2NA;

[0045] Bioprotective ingredient Supferm HIBY-PLUS:

[0046] The preparation process of Supferm HIBY-PLUS is as follows: preparing highland barley and water as fermentation substrates, with a material-liquid ratio of 1:200-1:100, sterilizing with high temperature and high pressure, cooling, and inoculating yeast; fermenting at a fermentation temperature of 27-30° C.; after the fermentation is completed, removing impurities, taking the filtrate, and obtaining highland barley fermentation liquid.

[0047] Supferm HIBY-PLUS has excellent DPPH free radical scavenging ability, reducing ability, oxygen free radical absorption ability and cellular antioxidant activity.

[0048] Recomtein AAG Time Freezing Origin:

[0049] The preparation process of Recomtein AAG is as follows: the full-length or partial amino acid sequence fragment encoded by the human collagen specific type gene prepared by DNA recombination technology, or a combination of human collagen functional fragments. Through the RGD site-specific gene editing technology, RGD is introduced into the recombinant protein to obtain a human-like water-soluble biological collagen. Collagen is widely used in various fields due to its excellent biocompatibility and versatility. Compared with animal collagen, recombinant collagen has a single component, high safety, controllable production process, better activity, and can be better used in anti-aging and firming effects.

[0050] Recomtein AAG has a strong structural stability and exists in a single helical linear structure with a triple helix structure in aqueous solution. It is easy to store and transport, has high protein purity, and maintains cell adhesion; Recomtein AAG has the effect of promoting skin remodeling, enhancing elasticity, and making it more firm. Therefore, this sunscreen can reduce the damage of photoaging to the skin by adding Recomtein AAG.

[0051] Verification of recombinant humanized collagen against photoaging

[0052] Skin aging is influenced by exogenous factors, and ultraviolet rays in particular cause premature skin aging, also known as extrinsic skin aging or photoaging, which is the main cause of changes associated with the aging process in areas exposed to the sun. [1] Skin wrinkles are caused by the reduction of extracellular matrix components (such as collagen) and are the main external manifestation of photoaging. Collagen is mainly produced by fibroblasts and is the main component of the extracellular matrix.

[0053] By using genetic engineering methods to combine collagen fragments with fibronectin fragments, a new polypeptide fusion protein, FCC protein, is formed. FCC protein has the characteristics of both collagen and fibronectin. It is safe and non-toxic, and plays an important role in promoting cell adhesion, migration and tissue repair. FCC recombinant protein has good anti-photoaging effects both in vivo and in vitro.

[0054] Type I collagen is the most abundant collagen and can be degraded by MMP-1 and MMP-3. When the degradation of normal collagen and the accumulation of abnormal collagen fiber fragments increase, the proliferation of fibroblasts is inhibited, resulting in a decrease in collagen synthesis. UVB exposure leads to upregulation of MMP-1 protein expression and decreased collagen expression, which was also confirmed in this study. In addition, the inhibitory effect of FCC protein on UVB-induced fibroblast MMP-1 protein expression and the promoting effect on COLIII and Fibrillin protein expression were also confirmed. FCC protein can reduce UVB damage to the skin. Therefore, FCC protein may protect the skin from photoaging and delay the cellular aging process by inhibiting the expression of matrix metalloproteinases and inhibiting the degradation of extracellular matrix. The results are shown in Figure 2. Figure 1 and Figure 2 shown.

[0055] (1) RT-qPCR

[0056] MMP1 is matrix metalloproteinase 1, and MMP3 is matrix metalloproteinase 3. When the expression of both is upregulated, they can promote the degradation of the extracellular matrix ECM. SMP30 is a cell senescence marker protein. When cells become aged, the expression of SMP30 will be upregulated. The results of the FCC anti-photoaging qPCR experiment are as follows Figure 1 As shown in the figure, when normal cells were irradiated with UVB, the expression of genes COLIII, Elastin and Fibrillin, which constitute the extracellular matrix components, was significantly reduced, while the addition of FCC and RHC significantly improved, indicating that FCC has a protective effect on the decomposition of extracellular matrix ECM after UVB radiation. In addition, after UVB irradiation, the genes MMP-3 and MMP-1 related to the degradation of extracellular matrix ECM showed an up-regulation trend, indicating that the cells were damaged and the synthesis of extracellular matrix ECM was inhibited. When FCC protein was added, there was no significant difference in the normal control group, indicating that FCC had no inhibitory effect on the secretion and synthesis of extracellular matrix; in addition, the SMP30 aging gene showed a significant down-regulation trend. It can be seen that the unilateral addition of FCC can improve the skin's ability to resist photoaging, and compared with the RHC group, the FCC group has a better anti-aging effect, proving that FCC protein can reduce the damage of skin to UVB.

[0057] (2) Western Blot

[0058] The results of WB experiment on FCC protein against photoaging are as follows Figure 2 As shown in the figure, it can be seen that the expression levels of COLⅢ and COLⅠ in the group irradiated by UVB lamp are lower than those in the Ctrl group, while the COLⅢ and COLⅠ in the FCC treatment group are basically the same as those in the Ctrl group, but the COLⅢ and COLⅠ protein expression levels in the RHC group and the animal collagen group are lower, which may be because the exogenously added RHC and animal collagen inhibit the secretion in the cells. In addition, the expression of MMP-3 and SMP30 in the groups after UVB modeling were significantly upregulated. After FCC treatment, the expression levels of MMP-3 and SMP30 were lower than those in the UVB group. This shows that FCC can alleviate the damage of cell metal matrix proteases to cells and delay the aging process of cells.

[0059] Features of AAG anti-sun pure zinc oxide sunscreen products:

[0060] 1. Recomtein AAG: Ultraviolet rays are one of the main causes of skin photoaging. By adding Recomtein AAG, the damage of photoaging to the skin can be reduced.

[0061] 2. Anti-photoaging: Ultraviolet rays are one of the main causes of skin photoaging. AAG chronolytic enzyme can reduce the damage of photoaging to the skin.

[0062] 3. Pure zinc oxide physical sunscreen: Pure zinc oxide is a physical sunscreen that can directly reflect and scatter ultraviolet rays to achieve a sunscreen effect. Compared with chemical sunscreens, pure zinc oxide sunscreen is milder and less likely to cause allergic reactions.

[0063] Compared with the prior art, the advantages of the present invention are:

[0064] 1. Comprehensive protection: The combination of collagen and pure zinc oxide provides comprehensive skin protection against sunburn and photoaging caused by UV rays.

[0065] 2. Anti-aging: helps reduce the damage of photoaging to the skin and keep the skin young.

[0066] 3. Gentle and safe: Pure zinc oxide is used as a physical sunscreen, which is not easy to cause allergic reactions and is suitable for sensitive skin.

[0067] 4. High quality: Ensure product quality and stability through strict formula research and development and production process.

[0068] 5. In order to increase the sun protection value in the prior art, more sunscreen is used, and thus more heavy oils need to be added to dissolve the sunscreen, which will make the skin feel heavy. The light stabilizer is added in this application with the purpose of using less sunscreen to have a higher sun protection value. With less sunscreen, less heavy oil will be added, and the skin feel will be lighter. BRIEF DESCRIPTION OF THE DRAWINGS

[0069] Figure 1 This is the expression chart of extracellular matrix-related genes and cellular inflammation and aging-related genes detected by RT-qPCR after drug administration.

[0070] Figure 2 This is a graph showing the expression of extracellular matrix-related proteins and cellular inflammation and aging-related proteins after drug administration detected by WB.

[0071] Figure 3 This is a normal light microscope image of Example 1.

[0072] Figure 4 This is the polarized light microscope image of Example 1.

[0073] Figure 5 This is the normal light microscope image of Comparative Example 1.

[0074] Figure 6 This is the polarized light microscope image of Comparative Example 1.

[0075] Figure 7 It is a comparison diagram of the ultraviolet absorption curves of Example 1 and Comparative Example 1. DETAILED DESCRIPTION

[0076] Prepare cosmetics according to the formula in Table 1:

[0077] Table 1

[0078]

[0079] In this example, Recomtein AAG was purchased from Guangzhou Jikemei Chuang Life Sciences Co., Ltd.;

[0080] Solapure ZnO C (zinc oxide surface treated with triethoxyoctylsilane) was purchased from Haosite New Materials Technology (Suzhou) Co., Ltd.;

[0081] The preparation method of highland barley fermentation liquid Supferm HIBY-PLUS in this embodiment is as follows: prepare highland barley and water as fermentation substrates, the material-liquid ratio is: 1:200-1:100, sterilize at high temperature and high pressure, cool down, and inoculate yeast; ferment, the fermentation temperature is: 27-30°C; after the fermentation is completed, remove impurities, take the filtrate, and obtain highland barley fermentation liquid.

[0082] In this embodiment, CRODAMOL AB-LQ-(RB) is C12-15 alcohol benzoate.

[0083] Example 1

[0084] Step 1: BHB (3%), KF-6115, KF-995 and Solapure ZnO C were stirred, dispersed and wetted, and then ground in a three-roll mill until uniformly dispersed to obtain component A;

[0085] Step 2: BHB (5%), KF-56A, and zinc stearate are heated and dissolved to obtain component B; Step 3: KF-6038, KF-6048, After weighing, mix evenly to obtain component C, pour component A and component B into component C, and place in a 70-degree water bath to keep warm;

[0086] Step 4: Heat the solvent of cinnamic acid ketone and 1,3-butanediol until the solvent is transparent and free of particles, then add EDTA-2NA, sodium chloride, 1,2-hexanediol and deionized water, place in a 70-degree water bath, stir and dissolve evenly until there are no particles, and then obtain component D;

[0087] Step 5: Mix Supferm HIBY-PLUS and AAG to obtain component E;

[0088] Step 6: Homogenize components A / B / C at 6000 rpm for 7 min; then slowly pour component D into components A / B / C under stirring at 6000 rpm for 5 min;

[0089] Step 7: After cooling to 45 degrees, add component E, and then cool to room temperature to obtain the target sunscreen product.

[0090] In this embodiment, the optimal ratio of Supferm HIBY-PLUS and AAG used as the bioprotective component is 10:1 by mass.

[0091] Comparative Example 1

[0092] Step 1: After CRODAMOL AB-LQ-(RB) (3%), KF-6115, KF-995 and Solapure ZnOC are stirred, dispersed and wetted, and then ground on a three-roll mill until they are uniformly dispersed to obtain component A;

[0093] Step 2: Heat and dissolve CRODAMOL AB-LQ-(RB) (5%), KF-56A and zinc stearate to obtain component B;

[0094] Step 3: Weigh KF-6038, KF-6048, and CRODAMOL AB-LQ-(RB) (3%) and mix them evenly to obtain component C. Pour component A and component B into component C and place in a 70-degree water bath to keep warm.

[0095] Step 4: Heat the solvent of cinnamic acid ketone and 1,3-butanediol until the solvent is transparent and free of particles, then add EDTA-2NA, sodium chloride, 1,2-hexanediol and deionized water, place in a 70-degree water bath, stir and dissolve evenly until there are no particles, and keep warm to obtain component D;

[0096] Step 5: Mix Supferm HIBY-PLUS and AAG to obtain component E;

[0097] Step 6: Homogenize components A / B / C at 6000 rpm for 7 min; then slowly pour component D into components A / B / C under stirring at 6000 rpm for 5 min;

[0098] Step 7: After cooling to 45 degrees, add component E, and then cool to room temperature to obtain the target sunscreen product.

[0099] The optimal ratio of Supferm HIBY-PLUS and AAG used as the bioprotection components in this control example is 10:1 by mass.

[0100] From the analysis of Example 1 and Comparative Example 1, it can be concluded that the stability of the example is good and has passed the stability test; the sunscreen value is high and the product safety is relatively high. However, no light stabilizer is added to Comparative Example 1, which affects the sunscreen value of the sunscreen product and causes uneven material. Example: Adding light stabilizer, the physical sunscreen agent is dispersed more evenly (such as Figure 3 and Figure 4 microscope), the sun protection value is higher (such as Figure 7 UV absorption curve comparison chart)

[0101] Comparative Example 1: No light stabilizer was added, and physical sunscreen was more likely to aggregate (microscope as follows Figure 5 and Figure 6 microscope), the sun protection value is low (such as Figure 7 UV absorption curve comparison chart)

[0102] In order to demonstrate the excellent effect of the present invention, the inventors conducted various performance tests on the new combination sunscreen lotion prepared in Example 1. The specific tests are as follows:

[0103] 1. Testing of the physical and chemical properties of sunscreen lotion:

[0104] a) The pH value of the sunscreen lotion measured by a pH meter is 6.0-7.0; b) The viscosity of the sunscreen lotion is measured by a rheometer, and the viscosity is 2000-3000 mPa·s; c) Stability test under external force: the sunscreen lotion is centrifuged at a speed of 3000 r / min for 30 minutes, and the water-oil phase separation of the sunscreen lotion is observed. The results show that the sunscreen lotion has no phase separation; d) Heat resistance, cold resistance, and cycle experiment: the sunscreen lotion is placed in an environment of heat resistance at 45℃ for 90 days, cold resistance at -18℃ for 90 days, and 24h 45℃, 24h room temperature, and 24h -18℃ (a total of 3 cycles) to observe the separation of the water phase and the oil phase of the sunscreen lotion. The results show that under the environment of hot and cold for 90 days and 3 cycles of hot and cold cycles, the sunscreen lotion has no phase separation. The specific physical and chemical basic properties are shown in Table 2.

[0105] Table 2 Basic physical and chemical properties of the new combination sunscreen lotion in Example 1

[0106] No parameter Observation under certain conditions 1 color milky 2 odor Characteristic smell, acceptable 3 Phase separation No phase separation 4 Centrifuge test Homogeneous, no phase separation 5 pH 6.0～7.0 6 Heat resistance test 45℃, no phase separation after 90 days 7 Cold resistance test -18℃, no phase separation in 90 days 8 Cycle Test 3 cycles, no phase separation for 9 days

[0107] AAG sunscreen patent efficacy test:

[0108] In terms of sun protection value, compared with the control group, after adding the photostabilizer in Example 1, the SPF value increased from 21.82 to 51.57, an increase of 136%, and the PA value increased from 8.26 to 10.08, an increase of 22%. It can be seen from the curve that the photostabilizer enhances the 290nm-360nm (UVA+UVB) band, and the enhancement strength in the UVB band is better. This shows that after adding the photostabilizer to the formula, even if a small amount of sunscreen is added, the sun protection value can be greatly improved to obtain a higher sun protection value.

[0109] Sample No. SPF PA Example 51.57 10.08 Comparative Example 21.82 8.26

[0110] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

Claims

1. A pure zinc oxide sunscreen containing recombinant collagen Recomtein AAG and capable of resisting photoaging, characterized in that: The composition includes, by mass percentage, 20-30% physical sunscreen, 10-15% light stabilizer, 4-6% emulsifier, 20-25% emollient, 1-3% dispersant, 0.1-2% thickener, 8-10% moisturizer, 0.1-1% preservative, 0.1-2% emulsion stabilizer, 0.1-1% chelating agent and 10-15% biological protection component, and the balance is water; The physical sunscreen is zinc oxide surface-treated with triethoxycaprylylsilane; The biological protection components include highland barley fermentation liquid Supferm HIBY-PLUS and Recomtein AAG coagulogen.

2. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The preparation method of the highland barley fermentation liquid Supferm HIBY-PLUS is as follows: preparing highland barley and water as fermentation substrates, with a material-liquid ratio of 1:200-1:100 g / mL, sterilizing with high temperature and high pressure, cooling, inoculating yeast; fermenting, with a fermentation temperature of 27-30°C; after the fermentation is completed, removing impurities, taking the filtrate, and obtaining highland barley fermentation liquid.

3. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The light stabilizer is one or more of acrylic acid (ester) copolymer or butyl octanol salicylate.

4. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The emulsifier is one or more of lauryl PEG-9, polydimethylsiloxyethyl, polydimethylsiloxane, and cetyl PEG / PPG.

5. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The emollient is one or more of cyclopentasiloxane or diphenylsiloxyphenyl trimethicone.

6. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The dispersant is lauryl polyglyceryl-3 polydimethylsiloxyethyl dimethicone.

7. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The thickener is zinc stearate; the moisturizer is one or more of butylene glycol and 1,2-hexanediol.

8. The pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging according to claim 1, characterized in that: The preservative is p-hydroxyacetophenone; the emulsifier stabilizer is sodium chloride; and the chelating agent is EDTA-2NA.

9. The method for preparing the pure zinc oxide sunscreen lotion containing recombinant collagen Recomtein AAG and capable of resisting photoaging as claimed in any one of claims 1 to 8, characterized in that: The steps include: Step 1: After the light stabilizer, emulsifier and physical sunscreen are stirred, dispersed and moistened, the mixture is ground until uniformly dispersed to obtain component A; Step 2: Heat and dissolve a light stabilizer, an emollient, and a thickener to obtain component B; Step 3: Take an emulsifier and a light stabilizer and weigh them and mix them evenly to obtain component C; pour component A and component B into component C and place them in a water bath at 65-75°C to keep warm; Step 4: Heat the solvent of xinxian ketone and 1,3-butanediol until it is transparent and free of particles, then add the chelating agent, sodium chloride, moisturizer, and deionized water, place in a 65-75°C water bath, stir and dissolve evenly until there are no particles, and then obtain component D; Step 5: Mix Supferm HIBY-PLUS with Recomtein AAG to obtain component E; Step 6: Homogenize components A / B / C at 5000-7000 rpm for 5-8 min; then slowly pour component D into components A / B / C under stirring at 5000-7000 rpm for 5-8 min; Step 7: After cooling to 40-50°C, add component E, and then cool to room temperature to obtain the target sunscreen product.

10. The preparation method according to claim 9, characterized in that: The mass ratio of Supferm HIBY-PLUS to RecomteinAAG is 10:1.

Images