Traditional Chinese medicine composition for treating camel mastitis and application

Abstract

The invention relates to a traditional Chinese medicine composition for treating camel mastitis and application, and relates to the technical field of veterinary medicine traditional Chinese medicine compositions. The traditional Chinese medicine composition is prepared from the following raw materials: honeysuckle flowers, dandelions, herba violae, fructus forsythiae, herba houttuyniae, liquorice roots and spica prunellae. The traditional Chinese medicine composition has a remarkable effect on camel mastitis, the curative effect is greatly higher than that of a single drug, the unique curative effect is achieved, and the problems that drug resistance is generated, toxic and side effects are large, the milk discarding period is long and the like due to the fact that the drug is used for treating mastitis in the prior art are effectively solved.

Description

Technical Field

[0001] The invention relates to the technical field of veterinary Chinese medicine compositions, and in particular to a Chinese medicine composition for treating camel mastitis and its application. Background Art

[0002] Mastitis is an inflammation of the breast caused by mechanical, physical, chemical and biological pathogenic factors. Its characteristics are: various inflammations of the mammary gland, milk cistern, nipple, and connective tissue between the mammary lobes, changes in the physical and chemical properties of milk and bacteriological changes (the presence of milk lumps, a large number of white blood cells and pathogenic microorganisms in the milk, and changes in the color of the milk). The clinical symptoms are mostly redness, swelling, heat, and pain in the breast, which also affects lactation, causing a decrease in milk production or even cessation of milk production.

[0003] Camel mastitis is the most harmful disease in the camel industry. The cause of camel mastitis is very complicated, and it increases the cost of treatment and management, reduces the quality of milk and production, and increases the elimination rate of female camels. The existing technology mainly uses antibacterial drugs to treat mastitis. Long-term use of antibiotics will make pathogens resistant, the efficacy will gradually decrease, and it will also bring greater toxic side effects to the camel's internal organs (liver, kidney, spleen, etc.); the use of antibacterial drugs for treatment will result in a long milk abandonment period and high treatment costs.

[0004] Chinese medicine formula has been widely used in veterinary clinics, and has the advantages of no drug residue, safety and good effect. However, at present, the treatment of camel mastitis with a single Chinese medicine cannot meet the needs of the development of modern breeding industry. Therefore, the development of a Chinese medicine composition that is effective in treating camel mastitis and low-cost is one of the new issues that scientific researchers in this field are urgently needed to solve. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a Chinese medicine composition and application for treating camel mastitis. The present invention effectively solves the problems of drug resistance, large toxic and side effects, long weaning period, high treatment cost, etc. caused by drug treatment of mastitis in the prior art.

[0006] The technical solution of the present invention to solve the above technical problems is as follows:

[0007] The first object of the present invention is to provide a traditional Chinese medicine composition for treating camel mastitis, which comprises the following raw materials: honeysuckle, dandelion, physalis, forsythia, houttuynia, liquorice and selfheal.

[0008] Honeysuckle: cold in nature, sweet in taste. It enters the lung meridian, heart meridian, and stomach meridian; it clears away heat and detoxifies, and cools and disperses wind-heat. It is a heat-clearing and detoxifying drug under the category of heat-clearing drugs. It has the effects of resisting pathogenic microorganisms, resisting toxins, resisting inflammation, relieving fever, and promoting the phagocytic function of inflammatory cells.

[0009] Dandelion: cold in nature, bitter and sweet in taste. Enters the liver meridian and stomach meridian. Clears away heat and detoxifies, diuretic and dissipates stagnation. It is a heat-clearing and detoxifying drug classified under heat-clearing drugs, and has anti-pathogenic microbial effects, bactericidal, anti-tumor, anti-gastric ulcer, bile-promoting and liver-protecting effects.

[0010] Herba Dianthus: cold in nature, bitter in taste, clears away heat and dampness, detoxifies and reduces swelling. Treats furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhea, red eyes, throat paralysis, and snake bites.

[0011] Forsythia suspensa: bitter and slightly cold in nature, it belongs to the lung, heart, and small intestine meridians. It has the effects of clearing away heat and detoxifying, eliminating carbuncle and dispersing nodules, and evacuating wind-heat.

[0012] Houttuynia cordata: slightly cold in nature, pungent in taste, with the effects of clearing away heat and detoxifying, eliminating carbuncle and draining pus, and promoting diuresis and relieving stranguria. It is mainly used to treat lung abscess with pus vomiting, lung heat cough, heat toxic sores and carbuncle, damp-heat stranguria, damp-heat diarrhea and other clinical symptoms, such as pneumonia, lung abscess, acute upper respiratory tract infection, etc.

[0013] Licorice: neutral in nature, sweet in taste. Enters the spleen, stomach, heart, and lung meridians. Raw, neutral in nature, clears away heat and detoxifies, and harmonizes various medicines; roasted with honey, slightly warm, nourishes the spleen and replenishes qi, moistens the lungs and relieves cough, and relieves pain.

[0014] Prunella Vulgaris: cold in nature, pungent and bitter in taste. It can clear the liver and purge fire, improve eyesight, dissipate stagnation and reduce swelling. It is used for red and swollen eyes, night pain in the eyes, headache and dizziness, scrofula, goiter, breast abscess, breast lumps, and breast pain.

[0015] The beneficial effects of the present invention are: the Chinese medicine composition of the present invention has a significant effect on camel mastitis, the curative effect is much higher than that of a single medicine, and has a unique curative effect. The Chinese medicine composition of the present invention is formed according to the compatibility principle of Chinese medicine, combined with the disease characteristics of camel mastitis, which is mainly dampness, qi, and blood stagnation, and the cure rate in clinical trials reaches more than 66.7%, has a unique curative effect, and can be used as a future alternative antibiotic product.

[0016] Furthermore, the mass ratio of the honeysuckle, the dandelion, the physalis, the forsythia, the houttuynia, the licorice, and the selfheal is (2.0-2.5): (2.0-2.5): (1.5-2.0): (1.5-2.0): (0.5-1.5): (0.5-1.5): (0.5-1.5).

[0017] The beneficial effect of adopting the above further scheme is that through the mutual cooperation between honeysuckle, dandelion, plantain, forsythia, houttuynia, licorice and selfheal, the prepared Chinese medicine composition can achieve the effects of clearing away heat and detoxifying, eliminating carbuncle and dispersing nodules, thereby achieving the effect of treating camel mastitis.

[0018] Preferably, the traditional Chinese medicine composition may be a composition of honeysuckle, dandelion and physalis, and the mass ratio of the honeysuckle, dandelion and physalis is 2:2:1. The therapeutic effect of the traditional Chinese medicine composition is poorer than that of the composition of honeysuckle, dandelion, physalis, forsythia, houttuynia cordata, licorice and selfheal, but the cost is lower and has a price advantage.

[0019] Furthermore, the mass ratio of the honeysuckle, the dandelion, the physalis, the forsythia, the houttuynia, the licorice, and the selfheal is 2:2:1.5:1.5:1:1:1.

[0020] The beneficial effect of adopting the above further scheme is: the proportion of raw materials in the traditional Chinese medicine composition is limited, so that the therapeutic effect of the composition on camel mastitis is optimized.

[0021] The second object of the present invention is to provide an application of a traditional Chinese medicine composition for treating camel mastitis, wherein the traditional Chinese medicine composition is used in preparing a therapeutic product for camel mastitis.

[0022] The beneficial effects of the present invention are as follows: the Chinese medicine composition of the present invention has a reasonable formula, a simple preparation process, a wide range of raw materials in the Chinese medicine composition, low cost, and a high cure rate; the medicine has no residue, no toxic side effects, and is easily absorbed by camels. It has a significant bactericidal and detoxifying effect, can effectively treat camel mastitis, and thus reduce the use of antibiotics.

[0023] Furthermore, the products include medicines and functional feeds.

[0024] Furthermore, the dosage form of the drug is a water extract.

[0025] The beneficial effects of adopting the above further scheme are as follows: the present invention uses the Chinese medicine composition as a medicine and feed to treat camel mastitis, wherein the medicine is used in the form of a water extract to treat camel mastitis, which is more conducive to the absorption of the effective ingredients in the Chinese medicine composition; when the treatment is carried out in the form of feed, no special treatment steps are required, which saves manpower and material resources, and does not destroy the activity of the effective ingredients of the medicine composition, and the treatment effect is better.

[0026] Furthermore, the preparation method of the drug is as follows:

[0027] The honeysuckle, dandelion, phytoncide, forsythia, houttuynia, licorice and selfheal are crushed, added with water and soaked for 2 hours, then boiled, and kept boiling for 30-45 minutes, the decoction and the residue are separated, and the decoction is concentrated to obtain the medicine.

[0028] Furthermore, the mass ratio of the water to the honeysuckle is 10:1.

[0029] Furthermore, the concentration temperature is 92-95°C.

[0030] Furthermore, the concentration time is 60-90 min.

[0031] Preferably, the concentration of the decoction is 1 g / mL. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The drawings are raw material drawings of the compound of the present invention; wherein (a) is the raw material drawing of the compound one in Examples 1 and 2; (b) is the raw material drawing of the compound two in Example 3; (c) is the raw material drawing of the compound three in Comparative Example 1; (d) is the raw material drawing of the compound four in Comparative Example 2;

[0033] Figure 2 The diagrams of camel udder changes after modeling in Experimental Example 3 of the present invention; wherein (a) is a diagram of typical changes in camel udder after modeling; (b) is a diagram of typical changes in camel milk after modeling; (c) is a diagram of typical changes in CMT detection of camel milk after modeling;

[0034] Figure 3 The diagrams are the changes of camel indicators before and after the challenge in Experimental Example 3 of the present invention; wherein (A) is the change diagram of skin thickness in the mammary area; (B) is the change diagram of body temperature in the camel; (C) is the change diagram of milk temperature in the camel;

[0035] Figure 4 The graphs of the changes in blood indicators of camels before and after modeling in Experimental Example 3 of the present invention are shown in Figure 3; wherein (A) is a graph showing the changes in the number of white blood cells; (B) is a graph showing the changes in the number of neutrophils; and (C) is a graph showing the changes in the number of lymphocytes.

[0036] Figure 5 The figure is a graph showing the change of lactic acid bacteria content before and after modeling in Experimental Example 3 of the present invention; wherein (a) is a low concentration group; (b) is a medium concentration group; and (c) is a high concentration group;

[0037] Figure 6 The diagrams of camel index changes before and after treatment in Experimental Example 4 of the present invention are shown in Figure 4; (a) is a diagram of skin thickness changes in the mammary area; (b) is a diagram of body temperature changes in the camel before and after treatment;

[0038] Figure 7 The graphs are the changes of blood indexes in camels before and after treatment in Experimental Example 4 of the present invention; (a) is the change graph of white blood cell count; (b) is the change graph of neutrophil count. DETAILED DESCRIPTION

[0039] The principles and features of the present invention are described below, and the examples are only used to explain the present invention and are not used to limit the scope of the present invention. If no specific technology or conditions are specified in the embodiments, the technology or conditions described in the literature in this field or the product instructions are used. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased through regular channels.

[0040] Example 1: Preparation of water extract

[0041] The raw materials of this embodiment (compound one) are as follows: 20g honeysuckle, 20g dandelion, 10g physalis;

[0042] Preparation method: grind 20g honeysuckle, 20g dandelion and 10g physalis, add 500mL water, soak for 2h and then boil, keep boiling for 30-45min, separate the decoction and the residue, and concentrate the decoction to obtain the water extract.

[0043] Example 2: Preparation of feed

[0044] The raw materials of this embodiment (compound one) are as follows: 200g honeysuckle, 200g dandelion, 100g physalis;

[0045] 200g of honeysuckle, 200g of dandelion and 100g of plantain are crushed and mixed to obtain feed.

[0046] Example 3: Preparation of water extract

[0047] The raw materials of this embodiment (compound 2) are as follows: 10g honeysuckle, 10g dandelion, 7.5g physalis, 7.5g forsythia, 5g houttuynia, 5g liquorice, 5g selfheal;

[0048] Preparation method: 10g honeysuckle, 10g dandelion, 7.5g physalis, 7.5g forsythia, 5g houttuynia, 5g licorice, and 5g selfheal are crushed, added with 500mL of water, soaked for 2h, then boiled, kept boiling for 45min, the decoction and the residue are separated, and the decoction is concentrated to obtain the water extract.

[0049] Example 4: Preparation of feed

[0050] The raw materials of the present embodiment are as follows: 100g honeysuckle, 100g dandelion, 75g physalis, 75g forsythia, 50g houttuynia, 50g liquorice, 50g selfheal;

[0051] Preparation method: 100g of honeysuckle, 100g of dandelion, 75g of physalis, 75g of forsythia, 50g of houttuynia, 50g of licorice and 50g of selfheal are crushed and mixed to obtain a mixture of the traditional Chinese medicine composition.

[0052] Comparative Example 1: Preparation of water extract

[0053] The raw materials of this comparative example (compound three) are as follows: 10g honeysuckle, 10g dandelion, 7.5g physalis, 7.5g coptis root, 5g purslane, 5g phellodendron, 5g trichosanthes;

[0054] Preparation method: grind 10g honeysuckle, 10g dandelion, 7.5g physalis, 7.5g coptis, 5g purslane, 5g phellodendron, and 5g trichosanthes, add 500mL of water, soak for 2h, then boil, keep boiling for 40min, separate the decoction and the residue, and concentrate the decoction to obtain the water extract.

[0055] Comparative Example 2: Preparation of water extract

[0056] The raw materials of this comparative example (compound 4) are as follows: 10g honeysuckle, 10g dandelion, 7.5g physalis, 7.5g scutellaria, 5g tuckahoe, 5g angelica, 5g tongcao;

[0057] Preparation method: grind 10g honeysuckle, 10g dandelion, 7.5g physalis, 7.5g scutellaria, 5g poria, 5g angelica, and 5g tongcao, add 500mL of water, soak for 2h, then boil, keep boiling for 40min, separate the decoction and the residue, and concentrate the decoction to obtain the water extract.

[0058] Experimental Example 1: Study on the antibacterial effect of Chinese herbal extracts on three pathogens of camel mastitis

[0059] The purpose of this experiment was to explore the in vitro antibacterial effects of 15 Chinese medicinal herbs, including Coptis chinensis, Viola yedoensis, Taraxacum mongolicum, Forsythia suspensa, Portulaca oleracea, Licorice root, Houttuynia cordata, Scutellaria baicalensis, Prunella vulgaris, Lonicera japonica, Phellodendron chinense, Angelica sinensis, Smilax glabra, Poria cocos, and Trichosanthes kirilowii, on three major pathogens of camel mastitis: Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli.

[0060] The test method is as follows:

[0061] Weigh 50 g each of Coptidis rhizome, Viola yedoensis, Taraxacum officinale, Fructus Forsythiae, Portulaca oleracea, Glycyrrhiza uralensis, Houttuynia cordata, Scutellaria baicalensis, Prunella vulgaris, Flos Lonicerae, Phellodendron chinense, Angelica sinensis, Herba Anemarrhenae, Poria cocos, and Fructus Trichosanthis, add 500 mL of water respectively, and prepare a water extract with a final concentration of 1 g / mL by the decoction method as in Example 1, then sterilize the water extract at 121° C. for 15 min, and perform in vitro antibacterial tests on the water extracts of 15 Chinese medicines using the Oxford cup method. The results are shown in Table 1.

[0062] Table 1: Antibacterial effects of 15 Chinese herbs on three pathogenic bacteria of camel mastitis

[0063]

[0064]

[0065] From Table 1, we can get:

[0066] The results of the inhibition zone diameters of 15 Chinese herbs on three pathogens of camel mastitis are shown in Table 1. It can be seen from Table 1 that the Chinese herbs with obvious antibacterial effects on Staphylococcus aureus are dandelion, purslane, coptis root, and scutellaria; the Chinese herbs with obvious antibacterial effects on Escherichia coli are dandelion, honeysuckle, physalis, and coptis root; the Chinese herbs with obvious antibacterial effects on Streptococcus agalactiae are houttuynia, coptis root, forsythia, phellodendron, tongcao, and physalis. Among them, physalis and honeysuckle have obvious antibacterial effects on the three pathogens.

[0067] Test Example 2: Results of in vitro antibacterial test of traditional Chinese medicine complex

[0068] Will be like Figure 1 The raw material compounds (Compound 1, Compound 2, Compound 3, Compound 4) of Example 1, Example 3, Comparative Example 1 and Comparative Example 2 were prepared by the decoction method as in Example 1 to prepare a water extract with a final concentration of 1 g / mL, and then the water extract was sterilized at 121° C. for 15 min, and the inhibition zone sizes of three pathogens of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli mixed in equal proportions were detected by the Oxford cup method, and the judgment criteria are shown in Table 2. The results of the inhibition zone diameters of the four traditional Chinese medicine compounds on pathogenic bacteria are shown in Table 3;

[0069] Table 2: Criteria for determining the antibacterial effect of Chinese herbal medicine extracts

[0070] Antibacterial degree Diameter of inhibition zone (mm) Poor antibacterial effect d＜6.00 Mild antibacterial 6.00≤d＜10.00 Moderate antibacterial 10.00≤d＜15.00 Highly antibacterial 15.00≤d＜20.00

[0071] Table 3: In vitro antibacterial effect of Chinese herbal compound (mm)

[0072] Group Result 1 Result 2 Result 3 Compound 1 14.63±0.28 14.6±0.24 14.09±0.13 Compound II 13.91±0.58 13.9±0.17 12.93±0.09 Compound Three 9.81±0.04 9.40±0.14 8.74±0.21 Compound IV 10.38±0.14 10.3±0.21 9.78±0.13

[0073] From Table 2, we can get:

[0074] Compound 1 and Compound 2 have certain antibacterial effects on pathogenic bacteria, and their antibacterial effects are moderate. Compound 3 and Compound 4 have poor antibacterial effects on pathogenic bacteria.

[0075] Test Example 3:

[0076] 1. Construction of camel bacterial mastitis model

[0077] (1) Model construction: Twelve healthy camels aged 7 to 8 years in the late lactation period were randomly divided into 4 groups, with 3 camels in each group. The left and right front mammary areas of each camel were selected as the experimental mammary areas. The first group was infused with a mixed bacterial solution (Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli mixed in a ratio of 1:1:1) at a rate of 2×10 5 CFU / mL as the low concentration group, and the second group was perfused with 2×10 6CFU / mL as the medium concentration group, and the third group was perfused with 2×10 7 CFU / mL bacterial solution was used as the high concentration group, each group was infused with 5mL of mixed bacterial solution, and the fourth group was infused with 5mL of normal saline as the control group. The injection was continued for 7 days to establish the camel bacterial mastitis model.

[0078] (2) Before and after modeling (i.e. before and after challenge), the clinical manifestations, udder appearance, and milk shape of the camels were observed by palpating the udder area, and the body temperature, udder area temperature, udder area skin thickness, milk bacteria content, milk CMT, and the number of white blood cells, neutrophils, and lymphocytes in the blood were measured. The detection method is as follows:

[0079] ① Breast skin thickness: Double electronic vernier calipers (Deli DL91150, Xinjiang Dingfeng Biotechnology Co., Ltd.) were used to measure the breast skin thickness before and after inoculation in each concentration group. The measurement time was fixed at 14:00-15:00 noon every day, and each peak was measured 3 times. There is no standard indicator for breast skin thickness, only the change trend is considered.

[0080] ②Body temperature measurement: Use a mercury thermometer to measure the Bactrian camel's body temperature in the rectum, and take it out after 5 minutes to read the corresponding temperature. (The standard temperature is 35.40-38.10℃ measured by the Gansu Veterinary Research Institute)

[0081] ③ Determination of model establishment: Use the California Mastitis Test (CMT) method to detect the model establishment. Dilute the CMT test solution (Xinjiang Dingfeng Biotechnology Co., Ltd.) with distilled water in a volume ratio of 1:3, discard the first three milks of the camel, collect 5mL of camel milk and place it in a plate on the diagnostic plate, and add an equal amount of CMT test solution to the camel milk. Shake the diagnostic plate quickly for about 10 to 20 seconds to fully mix the diagnostic solution and the milk sample, and determine the positive standard based on the degree of agglutination reaction. The determination method of the CMT method is as shown in Table 4:

[0082] Table 4

[0083]

[0084]

[0085] (4) Lactobacillus content: Milk was collected from each camel in each group, and 1 mL of the milk to be tested was diluted tenfold (1:1, 1:10, 1:100, 1:1000, 1:10000). 100 μL of milk was taken from each concentration group and spread on a solidified nutrient broth agar medium (10 g / L peptone, 3 g / L beef extract powder, 5 g / L sodium chloride, agar prepared at a ratio of 1.5%). The plate was incubated at 37°C for 18 h. It was appropriate to have 30-300 colonies on the plate. Three parallels were set for each concentration, and the lactobacillus content (CFU, Colony-Forming Units) was calculated to observe the changing trend of milk bacterial content after treatment.

[0086] (5) Blood indices: 5 mL of blood was collected from each camel in each group, and the number of white blood cells, neutrophils, and lymphocytes in the blood was measured using a veterinary blood cell analyzer (Exigo C200; Sweden).

[0087] The results are shown in Table 5. Figure 2-5 As shown;

[0088] Table 5: CMT test results of camel milk after virus attack

[0089]

[0090]

[0091] From Table 3, Figure 2-5 As shown:

[0092] (1) Camels that were perfused through the nipples with different bacterial concentrations did not show obvious systemic symptoms such as poor spirits, loss of appetite, and increased heart rate. Upon palpation of the perfused milk area, the camels in each experimental group showed different degrees of restlessness, wailing, pain, kicking the belly, and other resistance behaviors, while the camels in the control group showed no obvious symptoms, indicating that camels with mastitis have no obvious systemic symptoms, but have obvious pain symptoms when bacteria invade the local breast;

[0093] (2) Figure 2 As shown in the figure, compared with before the challenge, 3 days after the infusion of bacterial solution, the camels in the high-concentration group showed obvious udder redness and swelling, and the milk showed typical changes of paste-like, which was strongly positive by CMT test. The camels in other concentration groups did not show obvious udder redness and swelling, and the milk appeared granular and paste-like;

[0094] (3) Figure 3 , 4 As shown, after comparison with the control group, the body temperature, milk temperature, and skin thickness of the mammary area of ​​the camels in each experimental group before and after the challenge were significantly different, while the mammary area skin thickness of the camels in the high-concentration group showed differences after the challenge, and the mammary area of ​​the camels was significantly swollen, which was consistent with the clinical symptoms of mastitis;

[0095] (4) Figure 5 As shown in the figure, by comparing the inflammatory cell indicators in the camel blood before and after the challenge, it can be seen that the white blood cells, lymphocytes and neutrophils in the control group did not change significantly before and after the challenge. The number of inflammatory cells in each concentration group after the challenge showed significant differences compared with before the challenge, and the number of inflammatory cells in the high concentration group changed the most.

[0096] Test Example 4: Treatment of camel mastitis with a Chinese medicine composition

[0097] According to 2×10 7 CFU / mL concentration was used to reconstruct the mastitis model of 18 camels. The camels were randomly divided into 5 treatment groups and 1 control group, with 3 camels in each group. It was divided into an external use group and an oral group; the external use group used the water extracts prepared in Example 1 and Example 3 to scrub the nipple area; the oral group used the feed prepared in Example 2 and Example 4, with the dosage of 500g / peak / d and 500g / peak / d, respectively. In addition, an antibiotic group and a control group were set up, wherein the antibiotic group was intramuscularly injected with 50mL / peak / d, and the antibiotic selected was gentamicin (10mL: 0.2g; Shanxi Zhaoyi Biological Co., Ltd.), and the control group did not receive any treatment. Each group was treated for 15 days continuously. The clinical manifestations and udder appearance of the camels were observed by palpation of the udder area, and the milk state (combined with CMT observation) was observed. The thickness of the udder area, the content of milk bacteria, and the milk CMT were measured. 5 mL of blood was collected from each camel in each group, and the number of white blood cells and neutrophils contained was measured. The detection method was the same as that of Experimental Example 3: Cure rate = number of negative udder areas / total number of tested udder areas × 100%

[0098] The results are shown in Table 6-7. Figure 6-7 As shown;

[0099] Table 6: Clinical treatment effect statistics

[0100] Group Cure rate Compound for external use 50％(6 / 12) Compound oral 66.7％(8 / 12) Compound II for external use 33.3％(4 / 12) Compound 2 oral 83.3％(10 / 12) Antibiotic group 100％(12 / 12) Control group 16.7％(2 / 12)

[0101] Table 7: Results of bacterial counts in breast milk before and after treatment

[0102]

[0103]

[0104] Note: The same superscript indicates no significant difference (P>0.05)

[0105] Depend on Figure 6-7 , as shown in Table 6-7:

[0106] (1) As can be seen from Table 6, after 15 days of medication, clinical diagnosis and CMT testing revealed that the cure rate of the oral compound 1 group was 66.7%, the cure rate of the oral compound 2 group was 83.3%, and the cure rate of the antibiotic group was the highest (100%).

[0107] (2) As can be seen from Table 7, after 15 days of treatment, compared with the negative control group, the bacterial counts in the milk of the oral compound 1 group, the oral compound 2 group and the antibiotic group were significantly lower than those of the other groups, with significant differences (P<0.05). The oral compound 2 group was slightly better than the oral compound 1 group, but the difference was not significant (P>0.05).

[0108] (3) Before treatment, the skin thickness of the breast area in each group was greater than 11 mm, and there was no significant difference. After 9 days of treatment, the redness and swelling of the camel's breasts subsided, the sensitivity of the breast area was reduced, and there was no kicking or restlessness. Compared with the untreated group, the skin thickness of the breast area in the antibiotic group decreased significantly (P<0.05); there was no significant difference in body temperature among the groups (P>0.05). After 15 days of treatment, the skin thickness of the breast area in the compound 1 external application group, the compound 2 oral application group, and the antibiotic group decreased significantly (P<0.05), and the body temperature of each group recovered, but there was no difference (P>0.05).

[0109] (4) Before treatment, there were no significant differences in the number of white blood cells and neutrophils in each group. After 9 days of treatment, compared with the untreated group, the number of white blood cells in each treatment group was significantly reduced (P<0.05), among which the differences in the oral compound 1, external compound 2, oral compound 2, and antibiotic groups were extremely significant (P<0.01).

[0110] After 15 days of treatment, the number of white blood cells in the oral compound 1 and external compound 2 groups was significantly reduced compared with the untreated group (P<0.05); the difference in the reduction of white blood cell count between the oral compound 2 and antibiotic groups was extremely significant (P<0.01); no significant difference was found in the compound group (P>0.05).

[0111] (5) Before treatment, there was no significant difference in the number of neutrophils in each group. After 9 days of treatment, compared with the untreated group, the number of neutrophils in each treatment group was significantly reduced (P<0.05), and the difference between the oral compound II and antibiotic groups was extremely significant (P<0.01). After 15 days of treatment, compared with the untreated group, the number of neutrophils in each group showed a downward trend, and the difference between the oral compound II and antibiotic groups was significant (P<0.05). In summary, compound II can be used as the best antibiotic alternative formula for the treatment of camel mastitis, and its best route of administration is oral.

[0112] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A Chinese medicine composition for treating camel mastitis, characterized in that: The Chinese medicine composition comprises the following raw materials: Honeysuckle, dandelion, plantain, forsythia, houttuynia, licorice, and selfheal.

2. A Chinese medicine composition for treating camel mastitis according to claim 1, characterized in that: The mass ratio of the honeysuckle, the dandelion, the physalis, the forsythia, the houttuynia, the licorice and the selfheal is (2.0-2.5): (2.0-2.5): (1.5-2.0): (1.5-2.0): (0.5-1.5): (0.5-1.5): (0.5-1.5).

3. A Chinese medicine composition for treating camel mastitis according to claim 2, characterized in that: The mass ratio of the honeysuckle, the dandelion, the physalis, the forsythia, the houttuynia, the licorice, and the selfheal is 2:2:1.5:1.5:1:1:

1.

4. An application of a Chinese medicine composition for treating camel mastitis, characterized in that: The Chinese medicine composition according to any one of claims 1 to 3 is used to prepare a product for treating camel mastitis.

5. The use of a Chinese medicine composition for treating camel mastitis according to claim 4, characterized in that: The products include medicines and functional feeds.

6. The use of a Chinese medicine composition for treating camel mastitis according to claim 5, characterized in that: The dosage form of the medicine is water extract.

7. The use of a Chinese medicine composition for treating camel mastitis according to claim 6, characterized in that: The preparation method of the drug is specifically as follows: The honeysuckle, dandelion, phytoncide, forsythia, houttuynia, licorice and selfheal are crushed, soaked in water for 2 hours, boiled and kept boiling for 30-45 minutes, the decoction and the residue are separated, and the decoction is concentrated to obtain the medicine.

8. The use of a Chinese medicine composition for treating camel mastitis according to claim 7, characterized in that: The mass ratio of the water to the honeysuckle is 10:

1.

9. The use of a Chinese medicine composition for treating camel mastitis according to claim 7, characterized in that: The concentration temperature is 92-95°C.

10. The use of a Chinese medicine composition for treating camel mastitis according to claim 9, characterized in that: The concentration time is 60-90 min.

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