Cell active peptide composition for resisting acnes and regulating skin micro-ecology, preparation method of cell active peptide composition and application of cell active peptide composition in medicine and beauty

Abstract

The invention relates to a cell active peptide composition for resisting acnes and regulating skin micro-ecology, a preparation method thereof and application of the cell active peptide composition in medicine and beauty, and belongs to the technical field of medicine and beauty. The composition disclosed by the invention is prepared from a rhizoma coptidis extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, a lactobacillus fermentation extract and a bifidus yeast fermentation product lysate. The composition can balance peripheral androgen and reduce excessive secretion of sebum; hair follicle keratinization is removed, and pores are dredged; pathogenic bacterium infection is inhibited; acne injuries such as acne pits, acne marks, scars and pigmentation are repaired; the skin micro-ecology is balanced; the pores are shrunk. The composition is applied to skin beauty and skin medicine to effectively reduce the oil spot area, the oil intensity, the acne volume and the acne a value, and has the effects of resisting acnes and regulating the skin micro-ecology.

Description

Technical Field

[0001] The present invention belongs to the field of medicine and cosmetology technology, and specifically relates to a cell active peptide composition for resisting acne and regulating skin microecology, a preparation method thereof, and application thereof in medicine and cosmetology. Background Art

[0002] Skin, a vast organ covering our body, is not only our first line of defense against the world, but also an active metabolic participant, deeply involved in our body's metabolic activities. The skin is like a lush forest, full of vitality and diversity. The so-called "skin microecology" refers to the numerous microorganisms on the surface of the skin, including bacteria, fungi, viruses, etc., which interact with skin tissues, cells, secretions and microenvironment, forming a complex and delicate ecosystem.

[0003] These microorganisms on the skin, as non-specific antigens given to us by nature, can awaken and strengthen our body's immune system and strengthen our immune defenses. The symbiotic relationship between them and the skin not only promotes the healthy growth of skin cells, but also helps to delay skin aging and reduce the formation of wrinkles. Some resident bacteria on the skin can synthesize antibacterial, antifungal and antiviral substances, building a natural protective barrier for the skin and effectively resisting the invasion of harmful microorganisms.

[0004] However, if the skin is not properly cleaned, whether it is over-cleaned or under-cleaned, or is attacked by adverse external factors such as ultraviolet radiation, improper use of products containing irritating ingredients (such as hormones, fruit acids) or excessive additives in cosmetics, the balance of the skin's microecology will be disrupted. This imbalance will weaken the skin's original defenses, making the skin more susceptible to inflammation and infection, and becoming abnormally sensitive. This will lead to the formation of acne, weakening of the skin's barrier function, and discomfort such as dryness, tightness, itching, and even imbalance of the skin's water and oil balance.

[0005] Among them, acne can cause great discomfort to the skin. Its occurrence process includes the following steps: increased sebum secretion, blockage of the sebaceous gland ducts of the hair follicles, bacterial infection, and the proliferation of Propionibacterium acnes in the hair follicles in an anaerobic environment, which decomposes sebum and produces free fatty acids, thereby causing inflammation.

[0006] The occurrence of acne is closely related to the imbalance of skin microecology, but the existing technologies have not fully considered the comprehensive conditioning of skin microecology when treating acne. The following are some limitations of the existing technologies:

[0007] 1. Single treatment focus: Many treatments focus on reducing sebum secretion, controlling inflammation, or killing acne-related bacteria, while ignoring the overall balance of the skin microbiome.

[0008] 2. Ignoring microbial interactions: There are complex interactions between various microorganisms such as bacteria, fungi and viruses in the skin microbiome. The impact of these interactions on the development of acne is not fully considered.

[0009] 3. Excessive cleaning or use of antibiotics: Excessive cleaning of the skin or use of broad-spectrum antibiotics may destroy the normal microbial community of the skin, leading to microecological imbalance, which may aggravate acne.

[0010] 4. Ignoring the skin barrier function: In the process of treating acne, too much attention is paid to the lesions themselves, while the importance of maintaining and repairing the skin barrier function is ignored.

[0011] 5. Insufficient application of probiotics and prebiotics: The role of probiotics and prebiotics in regulating intestinal microecology has been widely recognized, but their application in the regulation of skin microecology is still in its infancy.

[0012] 6. Lack of strategies to maintain long-term balance of skin microecology: Lack of strategies to maintain long-term balance of skin microecology leads to easy recurrence of acne.

[0013] Therefore, in the face of recurrent acne problems and regulating skin microecology, it is necessary to continuously develop and improve new technologies to achieve faster, more effective and safer improvement results. Summary of the invention

[0014] The existing anti-acne products used in medicine and cosmetology have unsatisfactory effects, and even improper use may cause damage to the skin microecology, aggravation of acne, and repeated attacks of acne. The present invention provides a cell active peptide composition for anti-acne and regulating skin microecology, and a preparation method and application in medicine and cosmetology, the composition comprising coptis chinensis extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract, and bifid yeast fermentation product lysate.

[0015] The specific technical solutions of the present invention are as follows:

[0016] A cell active peptide composition for resisting acne and regulating skin microecology comprises the following raw materials in parts by mass: 0.0001 to 0.5 parts of coptis root extract, 0.0001 to 1 parts of hydrolyzed zinc hyaluronate, 0.0001 to 1 parts of azelaic acid, 0.0005 to 0.3 parts of nisin, 0.0001 to 0.05 parts of polylysine, 0.001 to 0.5 parts of palmitoyl tripeptide-8, 0.0001 to 0.5 parts of palmitoyl tripeptide-5, 0.001 to 1 parts of dipeptide-15, 0.0001 to 0.5 parts of capryloyl glycine, 0.0001 to 1 parts of lactic acid bacteria fermentation extract, and 0.0001 to 0.7 parts of bifid yeast fermentation product lysate.

[0017] The coptis chinensis extract is an extract with a value below 2 kDa obtained by sequentially enzymolysis of coptis chinensis rhizomes and snail protein peptides with cellulase, trypsin and nattokinase.

[0018] The lactic acid bacteria fermentation extract is an extract with a value below 2 kDa obtained by fermenting the stems and leaves of Lithospermum erythrorhizon, the stems and leaves of Centella asiatica and the leaves of Hamamelis virginiana with Lactobacillus acidophilus.

[0019] In the above technical scheme, the preparation method of the Coptis chinensis extract comprises the following steps: taking the rhizome of Coptis chinensis, crushing it, adding a saline solution with a mass of 10 to 12 times the mass of the rhizome of Coptis chinensis, adjusting the pH to 5.5 to 6.5, adding snail protein peptide and cellulase, enzymolysis at 40°C to 45°C for 3h to 4h, then adjusting the pH to 8.0 to 8.5, adding trypsin, enzymolysis at 36°C to 38°C for 50min to 80min, inactivating the enzyme at high temperature, cooling to room temperature, adjusting the pH to 8.0 to 9.0, adding nattokinase, enzymolysis at 40°C to 45°C for 60min to 90min, inactivating the enzyme at high temperature, cooling to room temperature, adjusting the pH to 6.5 to 7.0, centrifuging to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, freeze-drying, and obtaining a Coptis chinensis extract.

[0020] In the preparation method of the above-mentioned Coptis chinensis extract, the mass concentration of the saline solution is 1.0% to 2.5%; the added amounts of the snail protein peptide, cellulase, trypsin and nattokinase are respectively 0.3% to 1.0% of the mass of the Coptis chinensis rhizome.

[0021] In the preparation method of the above-mentioned Coptis chinensis extract, the enzyme activities of the cellulase, trypsin and nattokinase are all 50,000 U / g to 100,000 U / g.

[0022] In the preparation method of the above-mentioned Coptis chinensis extract, the temperature of high-temperature enzyme inactivation is 90°C to 95°C, and the time of high-temperature enzyme inactivation is 15min to 20min; the speed of centrifugal separation is 3000r / min to 4000r / min, and the time of centrifugal separation is 15min to 25min.

[0023] In the above technical scheme, the preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo lithospermum, the stems and leaves of Centella asiatica and the leaves of witch hazel according to the mass ratio of ginkgo lithospermum stems and leaves: Centella asiatica stems and leaves: witch hazel leaves = (3-6): (1-3): (0.5-1.5), crushing to obtain a mixture, adding water 10 to 15 times the mass of the mixture, 1% to 5% of the mass of yeast flavor in the mixture, and 1% to 3% of the mass of glucose in the mixture, adjusting the pH to 5.5 to 6.0, and ultrasonically mixing to obtain a mixed solution; adding Lactobacillus acidophilus to the mixed solution, fermenting anaerobically at 36°C to 38°C for 20h to 25h, sterilizing at high temperature, cooling to room temperature, adjusting the pH to 6.5 to 7.0, and centrifuging to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain a lactic acid bacteria fermentation extract.

[0024] In the preparation method of the above-mentioned lactic acid bacteria fermentation extract, the frequency of the ultrasound is 20kHz~30kHz, and the ultrasound time is 30min~40min; the temperature of the high-temperature sterilization is 121°C, and the sterilization time is 15min~20min; the speed of the centrifugal separation is 3000r / min~4000r / min, and the centrifugal separation time is 15min~25min.

[0025] In the preparation method of the lactic acid bacteria fermentation extract, the amount of Lactobacillus acidophilus added is 0.5% to 1.5% of the mass of the composition, and the bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g to 50 billion CFU / g.

[0026] The method for preparing the above-mentioned anti-acne, skin microecological regulating cell active peptide composition comprises the following steps:

[0027] The coptis root extract, hydrolyzed hyaluronate zinc, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract, and bifid yeast fermentation product lysate are added to 10 to 50 parts of purified water at a temperature of 40° C. to 45° C. by mass, the mixture is stirred and mixed evenly, freeze-dried, and sterilized to obtain a composition.

[0028] Application of the above-mentioned anti-acne and skin microecology regulating cell active peptide composition in medicine or cosmetology.

[0029] The above-mentioned cell active peptide composition for fighting acne and regulating skin microecology is used for preparing anti-acne products.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. The composition of the present invention includes coptis root extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract, and bifid yeast fermentation product lysate; each component is used in a specific proportion to reduce sebum secretion, effectively control oil, inhibit bacteria and reduce inflammation, remove follicle keratinization, balance peripheral androgens, quickly eliminate acne, repair acne scars and pigmentation, shrink pores, and regulate skin microecology. The anti-acne and skin microecology improvement effect is achieved from multiple links, and acne is not easy to recur.

[0032] 2. Coptis chinensis extract is an extract below 2kDa obtained by sequential enzymatic hydrolysis of Coptis chinensis rhizome and snail protein peptides with cellulase, trypsin, and nattokinase. It contains antibacterial substances and small molecule peptides, which not only has better antibacterial effect, but also has the functions of balancing peripheral androgens, balancing the flora ecology, repairing skin function, and preventing acne from recurring.

[0033] 3. Hydrolyzed zinc hyaluronate can inhibit the activity of 5α-reductase, which is related to sebum secretion. Therefore, hydrolyzed zinc hyaluronate has the function of regulating sebum secretion and realizing dynamic regulation of sebum.

[0034] 4. Azelaic acid has the effect of removing spots and acne and has excellent safety and efficacy.

[0035] 5. Nisin can bind to lipid II on the cell membrane of Gram-positive bacteria to form a nisin-lipid II complex, which in turn forms pores in the membrane, interfering with the bacterial energy metabolism and cell wall synthesis, thereby leading to bacterial death.

[0036] 6. Polylysine, also known as ε-polylysine, is produced by fermentation of glucose by Streptomyces albus. It contains 25 to 30 L-lysine units and has excellent broad-spectrum antibacterial ability.

[0037] 7. Palmitoyl tripeptide-8 can reduce the release of pro-inflammatory cytokines such as IL-1, IL-8 and TNF-α, help reduce vascular dilation and permeability, and reduce skin edema and erythema.

[0038] 8. Palmitoyl tripeptide-5 accelerates skin repair in inflammatory areas by increasing matrix cell activity, promoting the production of collagen, elastin, hyaluronic acid, glycosaminoglycans and fibronectin.

[0039] 9. Dipeptide-15 acts on glycine receptors, causing them to induce the influx of chloride ions, which helps to offset the excess positive charge of calcium ions in cells, thereby promoting the normal differentiation of keratinocytes and achieving the effect of improving enlarged pores.

[0040] 10. Capryloyl glycine can inhibit excessive secretion of sebum, help control oiliness and reduce the appearance of enlarged pores.

[0041] 11. Lactobacillus fermentation extract is an extract below 2kDa obtained by fermenting the stems and leaves of Ginkgo biloba, the stems and leaves of Centella asiatica and the leaves of Hamamelis virginiana with Lactobacillus acidophilus; through its rich active ingredients, it can not only maintain and regulate the balance of skin microecology, but also has good antibacterial properties against harmful bacteria such as Staphylococcus aureus, Candida albicans, Prevotella intermedia, Listeria, etc. It can promote the reproduction of Staphylococcus epidermidis and Corynebacterium, balance the antibacterial properties of azelaic acid on Staphylococcus epidermidis and Corynebacterium, thereby regulating the balance of skin microecology, allowing Staphylococcus epidermidis and Corynebacterium to play a microbial role, maintain the normal flora structure, and participate in immune regulation.

[0042] 12. The lysate of bifid yeast fermentation product can help maintain the microecological balance on the skin surface, promote the growth of beneficial bacteria and inhibit the reproduction of harmful bacteria. DETAILED DESCRIPTION

[0043] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0044] Example 1

[0045] A cell active peptide composition for resisting acne and regulating skin microecology comprises the following raw materials in parts by mass: 0.5 parts of coptis root extract, 1 part of hydrolyzed zinc hyaluronate, 1 part of azelaic acid, 0.3 parts of nisin, 0.05 parts of polylysine, 0.5 parts of palmitoyl tripeptide-8, 0.5 parts of palmitoyl tripeptide-5, 1 part of dipeptide-15, 0.5 parts of capryloyl glycine, 1 part of lactic acid bacteria fermentation extract, and 0.7 parts of bifid yeast fermentation product lysate.

[0046] The preparation method of the coptis chinensis extract comprises the following steps: taking coptis chinensis rhizomes, crushing them, adding a saline solution with a mass of 10 times the mass of the coptis chinensis rhizomes, wherein the mass concentration of the saline solution is 1.0%, then adjusting the pH to 5.5, adding 0.3% of snail protein peptide and cellulase by mass of the coptis chinensis rhizomes respectively, enzymolysis at 40°C for 3h, then adjusting the pH to 8.0, adding 0.3% of trypsin by mass of the coptis chinensis rhizomes, enzymolysis at 36°C for 50min, inactivating the enzyme at 90°C for 15min, cooling to room temperature, adjusting the pH to 8.0, adding 0.3% of nattokinase by mass of the coptis chinensis rhizomes, enzymolysis at 40°C for 60min, inactivating the enzyme at 90°C for 15min, cooling to room temperature, adjusting the pH to 6.5, and centrifuging at 3000r / min for 15min to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the coptis chinensis extract.

[0047] The enzyme activities of cellulase, trypsin and nattokinase are all 100,000 U / g.

[0048] The preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo lithospermum, the stems and leaves of Centella asiatica and the leaves of witch hazel in a mass ratio of 3:1:0.5, crushing to obtain a mixture, adding 10 times the mass of water, 1% of the mass of yeast flavor in the mixture, and 1% of the mass of glucose in the mixture, adjusting the pH to 5.5, and ultrasonically mixing at 20kHz for 30 minutes to obtain a mixed solution; adding 0.5% of the mass of Lactobacillus acidophilus of the composition to the mixed solution, fermenting anaerobically at 36°C for 20 hours, sterilizing at 121°C for 15 minutes, cooling to room temperature, adjusting the pH to 6.5, and centrifuging at 3000r / min for 15 minutes to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain a lactic acid bacteria fermentation extract.

[0049] The bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g.

[0050] The method for preparing the above-mentioned anti-acne, skin microecological regulating cell active peptide composition comprises the following steps:

[0051] According to the mass proportions, coptis root extract, hydrolyzed hyaluronate zinc, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract and bifid yeast fermentation product lysate are added to 50 parts of purified water at a temperature of 40°C, stirred and mixed evenly, freeze-dried and sterilized to obtain a composition.

[0052] Example 2

[0053] A cell active peptide composition for resisting acne and regulating skin microecology comprises the following raw materials in parts by mass: 0.3 parts of coptis root extract, 0.5 parts of hydrolyzed zinc hyaluronate, 0.3 parts of azelaic acid, 0.1 parts of nisin, 0.03 parts of polylysine, 0.2 parts of palmitoyl tripeptide-8, 0.3 parts of palmitoyl tripeptide-5, 0.5 parts of dipeptide-15, 0.3 parts of capryloyl glycine, 0.5 parts of lactic acid bacteria fermentation extract, and 0.5 parts of bifid yeast fermentation product lysate.

[0054] The preparation method of the coptis chinensis extract comprises the following steps: taking coptis chinensis rhizomes, crushing them, adding a saline solution with a mass of 11 times the mass of the coptis chinensis rhizomes, the mass concentration of the saline solution being 1.8%, then adjusting the pH to 6.0, adding 0.6% of snail protein peptide and cellulase by mass of the coptis chinensis rhizomes respectively, enzymolysis at 42°C for 3.5h, then adjusting the pH to 8.2, adding 0.5% of trypsin by mass of the coptis chinensis rhizomes, enzymolysis at 37°C for 60min, inactivating the enzyme at 92°C for 16min, cooling to room temperature, adjusting the pH to 8.5, adding 0.5% of nattokinase by mass of the coptis chinensis rhizomes, enzymolysis at 42°C for 80min, inactivating the enzyme at 92°C for 16min, cooling to room temperature, adjusting the pH to 6.8, and centrifuging at 3500r / min for 20min to obtain a supernatant; filtering the supernatant using a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the coptis chinensis extract.

[0055] The enzyme activities of cellulase, trypsin and nattokinase are all 100,000 U / g.

[0056] The preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo lithospermum, the stems and leaves of Centella asiatica and the leaves of witch hazel in a mass ratio of 5:2:1, crushing to obtain a mixture, adding 12 times the mass of water, 3% of the mass of yeast flavor in the mixture, and 2% of the mass of glucose in the mixture, adjusting the pH to 5.8, and ultrasonically mixing at 25kHz for 35min to obtain a mixed solution; adding 1.2% of the mass of Lactobacillus acidophilus of the composition to the mixed solution, fermenting anaerobically at 37°C for 22h, sterilizing at 121°C for 16min, cooling to room temperature, adjusting the pH to 6.8, and centrifuging at 3500r / min for 20min to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the lactic acid bacteria fermentation extract.

[0057] The bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g.

[0058] The method for preparing the above-mentioned anti-acne, skin microecological regulating cell active peptide composition comprises the following steps:

[0059] According to the mass proportions, coptis root extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract and bifid yeast fermentation product lysate were added to 45 parts of purified water at a temperature of 42°C, stirred and mixed evenly, freeze-dried and sterilized to obtain a composition.

[0060] Example 3

[0061] A cell active peptide composition for resisting acne and regulating skin microecology comprises the following raw materials in parts by mass: 0.05 parts of coptis root extract, 0.05 parts of hydrolyzed zinc hyaluronate, 0.05 parts of azelaic acid, 0.05 parts of nisin, 0.005 parts of polylysine, 0.05 parts of palmitoyl tripeptide-8, 0.05 parts of palmitoyl tripeptide-5, 0.05 parts of dipeptide-15, 0.03 parts of capryloyl glycine, 0.03 parts of lactic acid bacteria fermentation extract, and 0.02 parts of bifid yeast fermentation product lysate.

[0062] The preparation method of the coptis chinensis extract comprises the following steps: taking coptis chinensis rhizomes, crushing them, adding a saline solution with a mass of 12 times the mass of the coptis chinensis rhizomes, wherein the mass concentration of the saline solution is 2.5%, then adjusting the pH to 6.5, adding 1.0% by mass of snail protein peptide and cellulase to the coptis chinensis rhizomes respectively, performing enzymolysis at 45°C for 4h, then adjusting the pH to 8.5, adding 1.0% by mass of trypsin to the coptis chinensis rhizomes, performing enzymolysis at 38°C for 80min, inactivating the enzyme at 95°C for 20min, cooling to room temperature, adjusting the pH to 9.0, adding 1.0% by mass of nattokinase to the coptis chinensis rhizomes, performing enzymolysis at 45°C for 90min, inactivating the enzyme at 95°C for 20min, cooling to room temperature, adjusting the pH to 7.0, and centrifuging at 4000r / min for 25min to obtain a supernatant; filtering the supernatant using a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the coptis chinensis extract.

[0063] The enzyme activities of cellulase, trypsin and nattokinase are all 100,000 U / g.

[0064] The preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo lithospermum, the stems and leaves of Centella asiatica and the leaves of witch hazel in a mass ratio of 6:3:1.5, crushing to obtain a mixture, adding 15 times the mass of water, 5% of the mass of yeast flavor in the mixture, and 3% of the mass of glucose in the mixture, adjusting the pH to 6.0, and ultrasonically mixing at 30kHz for 40 minutes to obtain a mixed solution; adding 1.5% of the mass of Lactobacillus acidophilus of the composition to the mixed solution, fermenting anaerobically at 38°C for 25 hours, sterilizing at 121°C for 20 minutes, cooling to room temperature, adjusting the pH to 7.0, and centrifuging at 4000r / min for 25 minutes to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain a lactic acid bacteria fermentation extract.

[0065] The bacterial activity of Lactobacillus acidophilus is 50 billion CFU / g.

[0066] The method for preparing the above-mentioned anti-acne, skin microecological regulating cell active peptide composition comprises the following steps:

[0067] According to the mass proportions, coptis root extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract and bifid yeast fermentation product lysate are added to 40 parts of purified water at a temperature of 45°C, stirred and mixed evenly, freeze-dried and sterilized to obtain a composition.

[0068] Example 4

[0069] A cell active peptide composition for resisting acne and regulating skin microecology, comprising the following raw materials in parts by mass: 0.003 parts of coptis root extract, 0.002 parts of hydrolyzed hyaluronate zinc, 0.0008 parts of azelaic acid, 0.006 parts of nisin, 0.005 parts of polylysine, 0.006 parts of palmitoyl tripeptide-8, 0.005 parts of palmitoyl tripeptide-5, 0.008 parts of dipeptide-15, 0.004 parts of capryloyl glycine, 0.006 parts of lactic acid bacteria fermentation extract, and 0.005 parts of bifid yeast fermentation product lysate.

[0070] The preparation method of the coptis chinensis extract comprises the following steps: taking coptis chinensis rhizomes, crushing them, adding a saline solution with a mass of 10 times the mass of the coptis chinensis rhizomes, wherein the mass concentration of the saline solution is 2.5%, then adjusting the pH to 5.5, adding 1.0% of snail protein peptide and cellulase by mass of the coptis chinensis rhizomes respectively, performing enzymolysis at 40°C for 4h, then adjusting the pH to 8.0, adding 1.0% of trypsin by mass of the coptis chinensis rhizomes, performing enzymolysis at 36°C for 80min, inactivating the enzyme at 90°C for 20min, cooling to room temperature, adjusting the pH to 8.0, adding 1.0% of nattokinase by mass of the coptis chinensis rhizomes, performing enzymolysis at 40°C for 90min, inactivating the enzyme at 90°C for 20min, cooling to room temperature, adjusting the pH to 6.5, and centrifuging at 4000r / min for 15min to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the coptis chinensis extract.

[0071] The enzyme activities of cellulase, trypsin and nattokinase are all 100,000 U / g.

[0072] The preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo lithospermum, the stems and leaves of Centella asiatica and the leaves of witch hazel in a mass ratio of 3:3:0.5, crushing to obtain a mixture, adding 15 times the mass of water, 1% of the mass of yeast flavor in the mixture, and 3% of the mass of glucose in the mixture, adjusting the pH to 6.0, and ultrasonically mixing at 20kHz for 40 minutes to obtain a mixed solution; adding 1.5% of the mass of Lactobacillus acidophilus of the composition to the mixed solution, fermenting anaerobically at 36°C for 25 hours, sterilizing at 121°C for 15 minutes, cooling to room temperature, adjusting the pH to 7.0, and centrifuging at 3000r / min for 25 minutes to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain a lactic acid bacteria fermentation extract.

[0073] The bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g.

[0074] The method for preparing the above-mentioned anti-acne, skin microecological regulating cell active peptide composition comprises the following steps:

[0075] According to the mass proportions, coptis root extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract and bifid yeast fermentation product lysate are added to 20 parts of purified water at a temperature of 45°C, stirred and mixed evenly, freeze-dried and sterilized to obtain a composition.

[0076] Example 5

[0077] A cell active peptide composition for fighting acne and regulating skin microecology, comprising the following raw materials in parts by mass: 0.0001 parts of coptis root extract, 0.0001 parts of hydrolyzed zinc hyaluronate, 0.0001 parts of azelaic acid, 0.0005 parts of nisin, 0.0001 parts of polylysine, 0.001 parts of palmitoyl tripeptide-8, 0.0001 parts of palmitoyl tripeptide-5, 0.001 parts of dipeptide-15, 0.0001 parts of capryloyl glycine, 0.0001 parts of lactic acid bacteria fermentation extract, and 0.0001 parts of bifid yeast fermentation product lysate.

[0078] The preparation method of the coptis chinensis extract comprises the following steps: taking coptis chinensis rhizomes, crushing them, adding a saline solution with a mass of 12 times the mass of the coptis chinensis rhizomes, wherein the mass concentration of the saline solution is 1.0%, then adjusting the pH to 6.5, adding 0.3% of snail protein peptide and cellulase by mass of the coptis chinensis rhizomes respectively, enzymolysis at 45°C for 3h, then adjusting the pH to 8.5, adding 0.3% of trypsin by mass of the coptis chinensis rhizomes, enzymolysis at 38°C for 50min, inactivating the enzyme at 95°C for 15min, cooling to room temperature, adjusting the pH to 9.0, adding 0.3% of nattokinase by mass of the coptis chinensis rhizomes, enzymolysis at 45°C for 60min, inactivating the enzyme at 95°C for 15min, cooling to room temperature, adjusting the pH to 7.0, and centrifuging at 3000r / min for 25min to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the coptis chinensis extract.

[0079] The enzyme activities of cellulase, trypsin and nattokinase are all 50,000 U / g.

[0080] The preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo lithospermum, the stems and leaves of Centella asiatica and the leaves of witch hazel in a mass ratio of 6:1:1.5, crushing to obtain a mixture, adding 10 times the mass of water, 5% of the mass of yeast flavor in the mixture, and 1% of the mass of glucose in the mixture, adjusting the pH to 5.5, and ultrasonically mixing at 30kHz for 30 minutes to obtain a mixed solution; adding 0.5% of the mass of Lactobacillus acidophilus of the composition to the mixed solution, fermenting anaerobically at 38°C for 20 hours, sterilizing at 121°C for 20 minutes, cooling to room temperature, adjusting the pH to 6.5, and centrifuging at 4000r / min for 15 minutes to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain a lactic acid bacteria fermentation extract.

[0081] The bacterial activity of Lactobacillus acidophilus is 50 billion CFU / g.

[0082] The method for preparing the above-mentioned anti-acne, skin microecological regulating cell active peptide composition comprises the following steps:

[0083] According to the mass proportions, coptis root extract, hydrolyzed zinc hyaluronate, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract and bifid yeast fermentation product lysate are added to 10 parts of purified water at a temperature of 40°C, stirred and mixed evenly, freeze-dried and sterilized to obtain a composition.

[0084] Comparative Example 1

[0085] No Coptis chinensis extract was added to the composition; other parameters and methods were the same as in Example 1.

[0086] Comparative Example 2

[0087] No lactic acid bacteria fermentation extract was added to the composition; other parameters and methods were the same as in Example 1.

[0088] Comparative Example 3

[0089] No coptis root extract and lactic acid bacteria fermentation extract were added to the composition; other parameters and methods were the same as in Example 1.

[0090] Comparative Example 4

[0091] Lactic acid bacteria fermentation extract, nisin, and dipeptide-15 are not added to the composition; other parameters and methods are the same as in Example 1.

[0092] Comparative Example 5

[0093] In the preparation method of the Coptis chinensis extract, no snail protein peptide is added, and nattokinase is not used for enzymatic hydrolysis; other parameters and methods are the same as in Example 1.

[0094] Comparative Example 6

[0095] In the preparation method of the lactic acid bacteria fermentation extract, the stems and leaves of Ginkgo biloba are not added; other parameters and methods are the same as those in Example 1.

[0096] Comparative Example 7

[0097] In the preparation method of the lactic acid bacteria fermentation extract, no witch hazel leaves are added; other parameters and methods are the same as in Example 1.

[0098] Comparative Example 8

[0099] In the preparation method of the lactic acid bacteria fermentation extract, the stems and leaves of Lithospermum erythrorhizon and the leaves of Hamamelis virginiana are not added; other parameters and methods are the same as those in Example 1.

[0100] The compositions of the above embodiments and comparative examples were tested.

[0101] 1. Toxicity test

[0102] The acute oral toxicity test was conducted on mice, and 0.3g was gavaged and the reaction was observed. It was safe and non-toxic. The Ames test, mouse bone marrow cell micronucleus test, and mouse sperm abnormality test were all negative. Rats were fed with 0.2g / kg·bw / d, 0.5g / kg·bw / d, and 1g / kg·bw / d for 30 days, and there were no abnormal changes in the organs, and no harmful effects were observed.

[0103] 2. Anti-acne test

[0104] The composition was diluted with water to a 5 wt % sample solution; Propionibacterium acnes was diluted with water to 1.0×106 CFU / mL, and obtain the bacterial solution. Experimental group: add 100μL of bacterial solution to each well of a 96-well plate, and then add 100μL of sample solution. Positive control group: add 100μL of bacterial solution to each well of a 96-well plate, and then add 100μL of 250μg / mL erythromycin solution; blank group: add 100μL of bacterial solution to each well of a 96-well plate, and then add 100μL of purified water. Place in an anaerobic box, incubate for 48 hours, then take out, use an enzyme reader to detect the OD value, and calculate the antibacterial rate of the sample against Propionibacterium acnes. The results are shown in Table 1:

[0105] Table 1 Anti-acne efficacy test results

[0106]

[0107] It can be seen from the above results that the compositions of Examples 1 to 5 have a good antibacterial rate of Propionibacterium acnes and can effectively resist acne. It can be seen from the results of Comparative Example 1 that the Coptis chinensis extract has a certain inhibitory effect on Propionibacterium acnes. It can be seen from the results of Comparative Example 2 that the lactic acid bacteria fermentation extract has a certain inhibitory effect on Propionibacterium acnes. It can be seen from the results of Comparative Example 3 that without adding Coptis chinensis extract and lactic acid bacteria fermentation extract, the inhibitory effect of Propionibacterium acnes is greatly reduced, that is, Coptis chinensis extract and lactic acid bacteria fermentation extract are added at the same time in Example 1, and the inhibitory effect of Propionibacterium acnes is greatly improved, indicating that the use of Coptis chinensis extract and lactic acid bacteria fermentation extract has a good antibacterial synergistic effect. It can be seen from the results of Comparative Example 4 that the use of lactic acid bacteria fermentation extract, nisin, and dipeptide-15 has a good inhibitory effect on Propionibacterium acnes. It can be seen from the results of Comparative Example 5 that in the preparation method of Coptis chinensis extract, snail protein peptide is not added, and nattokinase is not used for enzymatic hydrolysis, and its antibacterial effect on Propionibacterium acnes is reduced. From the results of Comparative Examples 6 to 8, it can be seen that reducing the raw materials of the lactic acid bacteria fermentation extract, that is, not adding the stems and leaves of Ginkgo Biloba or the leaves of Hamamelis Virginiana, or not adding the stems and leaves of Ginkgo Biloba and the leaves of Hamamelis Virginiana at the same time, all have an impact on the composition of the lactic acid bacteria fermentation extract and reduce the antibacterial rate of Propionibacterium acnes.

[0108] 2. Antibacterial test of other harmful bacteria

[0109] The composition: purified water = 4:1 was mixed to prepare a paste sample. The bacterial solution of each strain was poured into a culture dish containing its own solid culture medium, the surface of the solid culture medium was infiltrated, and the paste was inoculated in the middle of the culture medium. Three parallel samples were prepared for each sample, and after 48 hours of culture, the diameter of the inhibition zone was measured, and the average value of the three parallel samples was taken. The results are shown in Table 2 below.

[0110] Table 2 Antibacterial test results (diameter of inhibition zone, mm)

[0111]

[0112] As can be seen from the above results, the compositions of Examples 1 to 5 have good antibacterial effects on harmful bacteria such as Staphylococcus aureus, Candida albicans, intermediate Prevotella, Listeria, etc., and have weaker antibacterial effects on Staphylococcus epidermidis and Corynebacterium, and can balance the microbial environment and contribute to balancing the microbial ecological balance. As can be seen from the results of Comparative Examples 1 to 3, Rhizoma Coptidis extract and lactic acid bacteria fermentation extract have certain antibacterial effects on harmful bacteria such as Staphylococcus aureus, Candida albicans, intermediate Prevotella, Listeria, etc. As can be seen from the results of Comparative Examples 6 to 8, reducing the raw materials of lactic acid bacteria fermentation extract, i.e., not adding galactoglobulus purpurogenum stems and leaves or witch hazel leaves, or not adding galactoglobulus purpurogenum stems and leaves and witch hazel leaves at the same time, all have an impact on the antibacterial effects of harmful bacteria such as Staphylococcus aureus, Candida albicans, intermediate Prevotella, Listeria, etc.

[0113] 4. Trial Evaluation

[0114] The composition was diluted with purified water to a concentration of 5 wt % to obtain a sample solution.

[0115] 78 subjects with moderate facial acne (each with more than 2 moderate acne) were selected and divided into 13 groups. After cleansing in the morning and evening, the sample solution was evenly applied to the face and absorbed for 5 minutes. The improvement of facial acne was continuously observed. The results are shown in Table 3 below.

[0116] Table 3 Improvement of facial acne (average value)

[0117]

[0118]

[0119] From the above results, it can be seen that the compositions of Examples 1 to 5 have good anti-acne effects, can quickly eliminate acne and repair acne marks, can balance the facial microbial environment, enhance antibacterial immunity, and effectively inhibit repeated infection by pathogenic bacteria. From the comparative examples, it can be seen that the preparation method with fewer ingredients or changed ingredients has a reduced efficacy in balancing the facial microbial environment, and acne is prone to recurrence.

[0120] The composition of the present invention includes coptis extract, hydrolyzed hyaluronate zinc, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract, bifid yeast fermentation product lysate; each component is used in combination to reduce sebum secretion, efficiently control oil, inhibit bacteria and reduce inflammation, remove follicular keratinization, balance peripheral androgens, quickly eliminate acne, repair acne scars and pigmentation, shrink pores, regulate skin microecology, and acne is not easy to recur. The present invention uses raw materials with different activities to prepare a cell activity composition, so that the composition can balance peripheral androgens, reduce excessive sebum secretion; remove follicular keratinization, dredge pores; inhibit pathogenic bacteria infection; repair acne pits, acne marks, scars, pigmentation and other acne damage; balance skin microecology; shrink pores. The composition is applied to skin beauty and dermatology to effectively reduce oil spot area, oil light intensity, acne volume, acne a value, and has an anti-acne effect of regulating skin microecology.

Claims

1. A cell active peptide composition for fighting acne and regulating skin microecology, characterized in that: The invention comprises the following raw materials in parts by weight: 0.0001 to 0.5 parts of coptis root extract, 0.0001 to 1 parts of hydrolyzed zinc hyaluronate, 0.0001 to 1 parts of azelaic acid, 0.0005 to 0.3 parts of nisin, 0.0001 to 0.05 parts of polylysine, 0.001 to 0.5 parts of palmitoyl tripeptide-8, 0.0001 to 0.5 parts of palmitoyl tripeptide-5, 0.001 to 1 parts of dipeptide-15, 0.0001 to 0.5 parts of capryloyl glycine, 0.0001 to 1 parts of lactic acid bacteria fermentation extract, and 0.0001 to 0.7 parts of bifid yeast fermentation product lysate; The coptis chinensis extract is an extract with a value below 2 kDa obtained by sequentially enzymolysis of coptis chinensis rhizome and snail protein peptide with cellulase, trypsin and nattokinase; The lactic acid bacteria fermentation extract is an extract with a value below 2 kDa obtained by fermenting the stems and leaves of Lithospermum erythrorhizon, the stems and leaves of Centella asiatica and the leaves of Hamamelis virginiana with Lactobacillus acidophilus.

2. The anti-acne, skin microecology regulating cell active peptide composition according to claim 1, characterized in that: The preparation method of the coptis chinensis extract comprises the following steps: taking coptis chinensis rhizomes, crushing them, adding a saline solution with a mass of 10 to 12 times the mass of the coptis chinensis rhizomes, adjusting the pH to 5.5 to 6.5, adding snail protein peptide and cellulase, performing enzymatic hydrolysis at 40 to 45°C for 3 to 4 hours, then adjusting the pH to 8.0 to 8.5, adding trypsin, performing enzymatic hydrolysis at 36 to 38°C for 50 to 80 minutes, inactivating the enzyme at high temperature, and adjusting the pH to 8.0 to 9.0 after cooling to room temperature, adding nattokinase, performing enzymatic hydrolysis at 40 to 45°C for 60 to 90 minutes, inactivating the enzyme at high temperature, and adjusting the pH to 6.5 to 7.0 after cooling to room temperature, and performing centrifugal separation to obtain a supernatant; filtering the supernatant using a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the coptis chinensis extract.

3. The anti-acne, skin microecology regulating cell active peptide composition according to claim 2, characterized in that: The mass concentration of the salt water solution is 1.0% to 2.5%; the added amounts of the snail protein peptide, cellulase, trypsin and nattokinase are 0.3% to 1.0% of the mass of the rhizome of Coptis chinensis respectively.

4. The anti-acne, skin microecology regulating cell active peptide composition according to claim 2, characterized in that: The enzyme activities of the cellulase, trypsin and nattokinase are all 50,000 U / g to 100,000 U / g.

5. The anti-acne, skin microecology regulating cell active peptide composition according to claim 2, characterized in that: The temperature of the high temperature enzyme inactivation is 90°C to 95°C, and the time of the high temperature enzyme inactivation is 15min to 20min; the speed of the centrifugal separation is 3000r / min to 4000r / min, and the time of the centrifugal separation is 15min to 25min.

6. The anti-acne, skin microecology regulating cell active peptide composition according to claim 1, characterized in that: The preparation method of the lactic acid bacteria fermentation extract comprises the following steps: mixing the stems and leaves of ginkgo purpurogenous root: the stems and leaves of Centella asiatica: the leaves of witch hazel according to the mass ratio of (3-6): (1-3): (0.5-1.5), crushing to obtain a mixture, adding water 10 to 15 times the mass of the mixture, 1% to 5% of the mass of yeast flavor in the mixture, and 1% to 3% of the mass of glucose in the mixture, adjusting the pH value to 5.5 to 6.0, and ultrasonically mixing to obtain a mixed solution; adding lactobacillus acidophilus to the mixed solution, fermenting anaerobically at 36 to 38°C for 20 to 25 hours, sterilizing at high temperature, adjusting the pH value to 6.5 to 7.0 after cooling to room temperature, and centrifuging to obtain a supernatant; filtering the supernatant with a 2kDa ultrafiltration membrane to obtain a filtrate below 2kDa, and freeze-drying to obtain the lactic acid bacteria fermentation extract.

7. The anti-acne, skin microecology regulating cell active peptide composition according to claim 6, characterized in that: The frequency of the ultrasound is 20kHz to 30kHz, and the ultrasound time is 30min to 40min; the temperature of the high-temperature sterilization is 121°C, and the sterilization time is 15min to 20min; the rotation speed of the centrifugal separation is 3000r / min to 4000r / min, and the centrifugal separation time is 15min to 25min.

8. The anti-acne, skin microecology regulating cell active peptide composition according to claim 6, characterized in that: The amount of Lactobacillus acidophilus added is 0.5% to 1.5% of the mass of the composition, and the bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g to 50 billion CFU / g.

9. The method for preparing a cell active peptide composition for fighting acne and regulating skin microecology according to claim 1, characterized in that: The preparation method comprises the following steps: The coptis root extract, hydrolyzed hyaluronate zinc, azelaic acid, nisin, polylysine, palmitoyl tripeptide-8, palmitoyl tripeptide-5, dipeptide-15, capryloyl glycine, lactic acid bacteria fermentation extract, and bifid yeast fermentation product lysate are added to 10 to 50 parts of purified water at a temperature of 40° C. to 45° C. by mass, the mixture is stirred and mixed evenly, freeze-dried, and sterilized to obtain a composition.

10. The use of a cell active peptide composition for fighting acne and regulating skin microecology according to claim 1, characterized in that: The composition is used to formulate anti-acne products.

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