Adherent cell culture reactor

Abstract

The invention relates to the field of cell culture equipment, in particular to an adherent cell culture reactor, and aims to solve the problems that some cells at the bottom of a culture dish are easy to lack nutrition when the cells are subjected to static culture at present, so that growth stagnation and even necrosis are caused; according to the adherent cell culture reactor provided by the invention, the problem that the cells are easily damaged and even die due to shearing force generated by stirring a culture solution through stirring equipment is solved, the adherent cell culture reactor is provided, and the adherent cell culture reactor consists of a left shell and a right shell which are symmetrically arranged and side plates which are fixedly connected between the two shells and are arranged on the front side and the rear side; the lower side of the vertical plate is provided with a driving structure for controlling the incubator to rotate at a steady speed, the inner walls of the two protective covers are respectively and fixedly connected with a culture bed, and the adherent cells can be driven to uniformly stay in the culture beds in the steady-speed rotation process of the incubator; the culture solution flows towards one direction in the steady-speed rotation process of the culture device, so that the shearing force generated during the circulation of the culture solution can be greatly reduced, and the damage to cells is reduced.

Description

Technical Field

[0001] The invention relates to the field of cell culture equipment, in particular to an adherent cell culture reactor. Background Art

[0002] Adherent cell culture is a commonly used cell culture technique, which refers to a technique of attaching cells to the wall of a culture (flask) vessel and culturing them in vitro. It is mainly based on the characteristic of cells to grow by adhering to the wall. Cells are placed in culture bottles, culture dishes, microcarriers, etc., so that the cells adhere to a fixed surface for growth. Adherent cell culture can be applied to cell culture operations including cell recovery, cell counting, cell passage, etc. This cell culture growth mode is closer to the natural growth state of cells in the body, which is conducive to studying the physiological functions and growth mechanisms of cells, and is conducive to observing and detecting changes in cell morphology, structure, function, etc., improving experimental efficiency. The cultured cells have a higher density, which can make better use of the culture space, and it is convenient to replace the culture medium, add drugs or stimulating factors, etc., and perform various experimental operations on the cells, so as to obtain more in-depth research data;

[0003] At present, in order to ensure the cell adhesion effect when culturing adherent cells, the cell suspension is usually transferred to a culture dish, and the culture dish is tilted to allow the cells to obtain a larger adhesion area, and then static culture is carried out. During the static culture process, since all the cells are precipitated and adsorbed on the inner wall of the culture dish, the cell density is too high, and some cells at the bottom cannot obtain sufficient nutrients. With the increase of metabolic waste, some cells at the bottom of the culture dish stagnate or even necrotize. The existing technology uses a stirring device such as an impeller to drive the circulation of the culture solution, promote the flow of cells to avoid accumulation, and then in the process of stirring the culture solution by the stirring device, the cells are directly in contact with the stirring device and the shear force generated during stirring can easily cause cell damage or even death, and is not conducive to the cell adhesion development. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides an adherent cell culture reactor, which effectively solves the problems mentioned in the above background technology.

[0005] In order to solve the above problems, the technical solution adopted by the present invention is:

[0006] A cell culture reactor for adherent cells comprises a bottom plate, a vertically arranged vertical plate is fixedly connected to the front side of the bottom plate, and a horizontally arranged culture vessel is rotatably connected to the rear side of the vertical plate; the culture vessel is composed of two symmetrically arranged shells on the left and right sides and side plates on the front and rear sides fixedly connected between the two shells, the side plate on the front side is fixedly connected and connected to a liquid inlet pipe, the liquid inlet pipe is rotatably connected to the vertical plate, a driving structure for controlling the steady-speed rotation of the culture vessel is installed on the lower side of the vertical plate, and the output end of the driving structure is connected to the liquid inlet pipe;

[0007] A partition is fixedly connected between the two shells, and the space between the two shells is divided into two upper and lower parts by the partition. Arc-shaped protective covers are respectively arranged on the upper and lower sides of the partition. The two protective covers are detachably connected between the two shells. A culture bed is fixedly connected to the inner walls of the two protective covers respectively. The culture beds are respectively in contact with the partition, and a plurality of through holes are respectively opened on the left and right sides of the surface of the partition.

[0008] Preferably, the partition plate is fixedly connected between the two shells in a state where the left side is lower and the right side is higher.

[0009] Preferably, an arc-shaped drainage tube is fixedly connected to the lower side of the through hole on the left side of the partition plate and the upper side of the through hole on the right side of the partition plate respectively.

[0010] Preferably, the circumferential surface of the shell is respectively fixedly connected with an annular circular plate, and the corresponding two sides of the circumferential surface of the circular plate are respectively provided with connecting grooves toward the inside, and the left and right sides of the upper end of the protective cover are respectively fixedly connected with the connecting plate, and the front and rear ends of the connecting plate are respectively provided with limited protrusions, and the corresponding two sides of the inner wall of the connecting groove are respectively provided with limited grooves, and the limiting protrusions and the limiting grooves are slidably matched; the inside of the connecting groove is respectively rotatably connected with a circular block, and the middle of the surface of the circular block is respectively fixedly connected with a connecting gear, and one side of the connecting gear is meshed with a connecting rack, and the upper end of the connecting rack is fixedly connected to the bottom of the connecting plate when the protective cover is installed. The meshing of the connecting rack and the connecting gear can drive the circular block to rotate counterclockwise. A locking structure for fixing the circular block is installed inside the connecting groove; a slide box is provided on the surface of the connecting rack, and the slide box is fixedly connected to the inner wall of the connecting groove, and a connecting slider is fixedly connected to the lower side of the surface of the connecting rack, and the connecting slider is slidably connected to the slide box, and a push spring is fixedly connected to the bottom of the connecting slider, and the other end of the push spring is fixedly connected to the inner wall of the slide box.

[0011] Preferably, the locking structure includes a card block rotatably connected to the inner wall of the connecting groove, the upper and lower ends of the card block are respectively provided with card grooves, the two sides corresponding to the circumferential surface of the circular block are respectively fixedly connected with a matching claw and a pushing claw, the surface of the circular block between the matching claw and the pushing claw is fixedly connected with a pushing block, and the inner end of the pushing claw is provided with a protrusion opened inward;

[0012] When the circular block rotates counterclockwise, the protrusion of the pushing claw can push the card block to rotate to a vertical state, so that the card slot of the card block corresponds to one corner of the push block, and then when the push spring pushes the connecting rack to move, the circular block is reversed under the meshing of the connecting rack and the connecting gear. When the circular block reverses, one corner of the push block is stuck in the card slot, so that the circular block cannot continue to rotate under the push of the push spring;

[0013] When the connecting rack drives the circular block to rotate counterclockwise again by meshing with the connecting gear, one corner of the push block disengages from the slot, and then the protrusion of the pushing claw pushes the block to rotate again to dislocate the slot and the push block, and then when the pushing spring pushes the connecting rack to move, the circular block is driven to reverse under the meshing of the connecting rack and the connecting gear. During the reversal of the circular block, the push block pushes the block to an inclined state and is placed in the space between the push block and the mating claw as the circular block continues to rotate.

[0014] Preferably, the outer end of the push block away from the circular block is in an arc-shaped structure, and the left and right ends of the clamping block are respectively provided with arc surface structures that can fit with the outer end of the push block.

[0015] Preferably, the internal sliding connection of the connecting groove is provided with a mounting slide block that can move vertically, the bottom of the mounting slide block is fixedly connected to a supporting spring, the lower end of the supporting spring is fixedly connected to the inner wall of the connecting groove, the rear end of the pushing claw is fixedly connected to a swing plate, the surface of the swing plate is provided with a matching groove along the length direction, the surface of the mounting slide block is fixedly connected to a first pin shaft, and the first pin shaft is slidingly fitted in the inner side of the matching groove.

[0016] Preferably, a bearing wheel is fixedly connected to the surface of the liquid inlet pipe, and an annular plate is rotatably connected to the circumferential surface of the bearing wheel. A friction wheel is mounted on the surface of the liquid inlet pipe, and a plurality of spring sheets are respectively fixedly connected to the circumferential surfaces of the friction wheel, and the other ends of the spring sheets are respectively fixedly connected to the annular plates. Under the pressure of the spring sheets, the friction wheel is connected to the bearing wheel, and a transmission tube is coaxially fixedly connected to the surface of the friction wheel, and the transmission tube is sleeved on the surface of the liquid inlet pipe, and a transmission sleeve is slidably connected to the surface of the transmission tube, and the transmission sleeve is rotatably connected to the vertical plate, and a worm wheel is fixedly connected to the surface of the transmission sleeve, and a worm is meshed with one side of the worm wheel, and the worm is rotatably connected to the vertical plate, and a driving motor is arranged on the lower side of the worm, and the driving motor is fixedly connected to the vertical plate, and the output end on the upper side of the driving motor is fixedly connected to the worm wheel.

[0017] Preferably, a linkage plate is rotatably connected to the surface of the transmission tube, a limit rod is slidably connected to the bottom of the linkage plate, the limit rod is fixedly connected to the vertical plate, and a second pin is fixedly connected to the right end of the linkage plate;

[0018] The surface of the vertical plate is vertically provided with an installation slide groove, and a linkage slide block is slidably connected inside the installation slide groove. The front and rear ends of the linkage slide block are respectively fixedly connected with a handle and a pressure rod, and the rear end of the pressure rod is fixedly connected with a guide plate, and the lower end of the linkage slide block is fixedly connected with a return spring, and the lower end of the return spring is fixedly connected to the lower end of the installation slide groove. A guide groove with a bottom end inclined toward the rear side is provided on the surface of the guide plate, and the second pin shaft slides with the bottom end of the guide groove. When the guide plate moves downward, the friction wheel can be driven to move forward and disengage from the bearing wheel through the sliding cooperation between the guide groove and the second pin shaft; a liquid outlet is provided on the surface of the shell at the right end, and a blocking bolt is threadedly connected to the liquid outlet.

[0019] Preferably, a ratchet is fixedly connected to the front side of the liquid inlet pipe, and a rotating disk is rotatably connected to the surface of the ratchet, and a plurality of ratchet pawls meshing with the ratchet are rotatably connected to the surface of the rotating disk, and the surfaces of the ratchet pawls are respectively fixedly connected to elastic sheets, and the other ends of the elastic sheets are respectively fixedly connected to the rotating disk under the push of the elastic sheets, and the ratchet pawls mesh with the ratchet wheels. A matching plate is fixedly connected to the surface of the rotating disk, and a vertical groove and an oblique groove are opened on the surface of the matching plate, and the bottom of the vertical groove and the oblique groove are connected, and the pressure rod slides and fits in the upper end of the vertical groove. The rotating disk cannot rotate under the sliding cooperation of the pressure rod and the vertical groove. When the rotating disk cannot rotate, the liquid inlet pipe can rotate unidirectionally under the meshing of the ratchet and the ratchet wheel without affecting the rotating disk. When the pressure rod moves downward, it can drive the rotating disk to rotate through the sliding cooperation with the oblique groove, and when the rotating disk rotates, the liquid inlet pipe is driven to rotate through the meshing of the ratchet and the ratchet wheel.

[0020] The present invention has novel structure, ingenious design, simple and convenient operation, and has the following advantages compared with the prior art:

[0021] 1. During the steady-speed rotation of the culture vessel, the adherent cells can be driven to stay evenly in the culture bed, avoiding the situation where the cells are too dense and cause cell death. In addition, since the culture medium flows in one direction during the steady-speed rotation of the culture vessel, the shear force generated during the circulation of the culture medium can be greatly reduced, reducing the damage to the cells;

[0022] 2. When installing the protective cover, the device can directly connect the connecting plate with the connecting groove and press the protective cover. After pressing the protective cover to the bottom, release the protective cover. The culture bed can be fixed on the culture device. When removing the culture bed, the protective cover can be pressed again. After releasing it, the protective cover can be taken out. The user can remove the culture bed, which is convenient for the user to remove the adherent cells. The operation is simple and convenient.

[0023] 3. When the user needs to pour out the culture solution, the handle can be directly controlled to move downward. When the handle moves downward, the guide plate can be driven downward by the pressure rod. Under the cooperation of the guide groove and the second pin shaft, the friction wheel moves forward and disconnects from the bearing wheel. Then, in the process of the pressure rod moving downward, the rotating disk is driven to rotate through the cooperation with the inclined groove. When the rotating disk rotates, the incubator is driven to rotate through the engagement of the pawl and the ratchet wheel, so that the liquid outlet discharges the culture solution downward, thereby improving the stability of controlling the discharge of the culture solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of an adherent cell culture reactor of the present invention.

[0025] Figure 2 This is a first schematic diagram of a protective cover installation structure of an adherent cell culture reactor of the present invention.

[0026] Figure 3 This is a second schematic diagram of the protective cover installation structure of an adherent cell culture reactor of the present invention.

[0027] Figure 4 The figure is a schematic diagram of the installation structure of the drainage tube of an adherent cell culture reactor of the present invention.

[0028] Figure 5 The figure is a schematic diagram of the installation structure of a partition plate of an adherent cell culture reactor of the present invention.

[0029] Figure 6 It is a first schematic diagram of the motion state of the locking structure of an adherent cell culture reactor of the present invention.

[0030] Figure 7 This is a second schematic diagram of the motion state of the locking structure of an adherent cell culture reactor of the present invention.

[0031] Figure 8 This is a third schematic diagram of the motion state of the locking structure of an adherent cell culture reactor of the present invention.

[0032] Fig. 9 This is a fourth schematic diagram of the motion state of the locking structure of an adherent cell culture reactor of the present invention.

[0033] Fig.10 It is a schematic diagram of the matching structure of a swing plate and a first pin shaft of an adherent cell culture reactor of the present invention.

[0034] Fig.11 The figure is a schematic diagram of the installation structure of the feed port of an adherent cell culture reactor of the present invention.

[0035] Fig.12The present invention is a schematic diagram of the cooperation structure of the friction wheel and the bearing wheel of an adherent cell culture reactor.

[0036] Fig.13 The figure is a schematic diagram of the transmission structure of a driving motor of an adherent cell culture reactor of the present invention.

[0037] Fig.14 The figure is a schematic diagram of the driving structure of a guide plate of an adherent cell culture reactor of the present invention.

[0038] Fig.15 The figure is a schematic diagram of the installation structure of a rotating disk of an adherent cell culture reactor of the present invention.

[0039] Fig.16 It is a schematic diagram of the handle installation structure of an adherent cell culture reactor of the present invention.

[0040] Numbers in the figure: 1-bottom plate, 2-vertical plate, 3-culture vessel, 4-shell, 5-side plate, 6-protective cover, 7-partition plate, 8-culture bed, 9-liquid inlet pipe, 10-through hole, 11-drainage pipe, 12-blocking bolt, 13-connecting plate, 14-connecting rack, 15-slide box, 16-round block, 17-connecting gear, 18-matching claw, 19-pushing claw, 20-block, 21-slot, 22-push block, 23-protrusion, 24-connecting slider, 25-pushing spring, 26-swinging plate, 27-matching groove, 28-installing slider, 29-first pin shaft, 30-support Support spring, 31-bearing wheel, 32-ball valve, 33-liquid inlet, 34-circular plate, 35-annular plate, 36-spring sheet, 37-friction wheel, 38-linking plate, 39-second pin, 40-limiting rod, 41-transmission pipe, 42-transmission sleeve, 43-worm gear, 44-worm, 45-drive motor, 47-rotating disk, 48-ratchet, 49-elastic sheet, 50-ratchet, 51-matching plate, 52-pressure rod, 53-linking slider, 54-reset spring, 55-guide plate, 56-guide groove, 57-handle, 58-vertical groove, 59-oblique groove, 60-liquid outlet. DETAILED DESCRIPTION

[0041] The following are specific embodiments of the present invention, and the technical solutions of the present invention are further described in conjunction with the accompanying drawings, but the present invention is not limited to these embodiments.

[0042] like Figure 1-16As shown, the present invention provides an adherent cell culture reactor, comprising a bottom plate 1, a vertically arranged upright plate 2 is fixedly connected to the front side of the bottom plate 1, and a horizontally arranged culture vessel 3 is rotatably connected to the rear side of the upright plate 2; the culture vessel 3 is composed of two symmetrically arranged shells 4 on the left and right and side plates 5 on the front and rear sides fixedly connected between the two shells 4, the front side plate 5 is fixedly connected and connected to a liquid inlet pipe 9, the liquid inlet pipe 9 is rotatably connected to the upright plate 2, and a driving structure for controlling the steady-speed rotation of the culture vessel 3 is installed on the lower side of the upright plate 2, and the output end of the driving structure is connected to the liquid inlet pipe 9;

[0043] A partition plate 7 is fixedly connected between the two shells 4, and the space between the two shells 4 is divided into two upper and lower parts by the partition plate 7. Arc-shaped protective covers 6 are respectively provided on the upper and lower sides of the partition plate 7. The two protective covers 6 are respectively detachably connected between the two shells 4. A culture bed 8 is respectively fixedly connected to the inner walls of the two protective covers 6. The culture beds 8 are respectively in contact with the partition plate 7. A plurality of through holes 10 are respectively opened on the left and right sides of the surface of the partition plate 7.

[0044] The incubator 3 is formed by the combination of two protective covers 6 and the cooperation of two shells 4 to form a accommodating space with hemispherical ends and cylindrical middle for accommodating culture fluid, and is separated into upper and lower spaces by a partition plate 7. The front end of the liquid inlet pipe 9 is rotatably connected to a ball valve 32, and the ball valve 32 is fixedly connected to the vertical plate 2. The front end opening of the ball valve 32 is fixedly connected to a cylindrical liquid inlet 33. When in use, the ball valve 32 can be opened, the device can be reversed to a state where the feed port is facing upward, the cell suspension tube feed port and the liquid inlet pipe 9 are injected into the incubator 3, and then the ball valve 32 is closed, and then the device is placed in an incubator with a constant temperature. Since the incubator 3 is in an initial state, the cell suspension in the incubator 3 is in a horizontal position, and the through holes 10 on both sides of the surface of the partition plate 7 are used to connect the upper and lower spaces. When culturing cells, the incubator 3 can be driven by the driving structure to rotate clockwise at a steady speed. Under the action of gravity, the culture fluid containing the cell suspension can be moved along with the incubator. The culture vessel 3 rotates clockwise for flow. When the culture vessel 3 rotates driven by the driving structure, the upper and lower spaces of the culture vessel 3 alternate repeatedly, and the culture fluid can flow through the upper and lower spaces repeatedly in one direction through the through hole 10. The culture bed 8 is composed of polyester fibers, which can provide a large number of attachments for the adherent cells, and there are gaps inside the culture bed 8 composed of polyester fibers, which is conducive to the circulation of the culture fluid. In the process of the culture fluid flowing through the culture bed 8, the adherent cells can be in the culture bed 8 and develop using the culture bed 8; in the process of the steady-speed rotation of the culture vessel 3, the adherent cells can be driven to stay evenly in the culture bed 8 to avoid the situation where the cells are too dense and cause cell death, and because the culture fluid flows in one direction during the steady-speed rotation of the culture vessel 3, the shear force generated when the culture fluid circulates can be greatly reduced, thereby reducing damage to the cells; the two protective covers 6 can be removed from the culture vessel 3, which is convenient for the user to remove the adherent cells inside.

[0045] The partition plate 7 is fixedly connected between the two shells 4 in a state of being lower on the left and higher on the right. Each time the culture vessel 3 is rotated to a horizontal state, the partition plate 7 is in a state of being lower on the left and higher on the right, so that when the culture vessel 3 is in a horizontal position, the culture fluid in the upper space can flow to the lower space through the through hole 10 on the left under the action of gravity, which is beneficial to promoting the circulation of the culture fluid.

[0046] Furthermore, in order to reduce the shear force generated when the culture device 3 rotates, the lower side of the through hole 10 on the left side of the partition plate 7 and the upper side of the through hole 10 on the right side of the partition plate 7 are respectively fixedly connected with an arc-shaped drainage tube 11, and the other end of the drainage tube 11 is respectively in contact with the culture bed 8, such as Figure 4 As shown, when the culture vessel 3 rotates clockwise, the drainage tube 11 can provide a circulation channel for the culture fluid, so that the culture fluid can flow directly to the culture bed 8, avoiding a large drop when the culture vessel 3 rotates and avoiding shear force caused by a large error from causing damage to the cells.

[0047] The circumferential surface of the shell 4 is respectively fixedly connected with an annular circular plate 34, and the corresponding two sides of the circumferential surface of the circular plate 35 are respectively provided with connecting grooves inwardly, and the left and right sides of the upper end of the protective cover 6 are respectively fixedly connected with the connecting plate 13, and the front and rear ends of the connecting plate 13 are respectively provided with limiting protrusions 23, and the corresponding two sides of the inner wall of the connecting groove are respectively provided with limiting grooves, and the limiting protrusions 23 are slidably matched with the limiting grooves; the inside of the connecting groove is respectively rotatably connected with a circular block 16, and the middle part of the surface of the circular block 16 is respectively fixedly connected with a connecting gear 17, and one side of the connecting gear 17 is meshed with a connecting rack 14, and the upper end of the connecting rack 14 is fixedly connected to the bottom of the connecting plate 13. When the protective cover 6 is installed, the circular block 16 can be driven to rotate counterclockwise by the meshing of the connecting rack 14 and the connecting gear 17, and a locking structure for fixing the circular block 16 is installed inside the connecting groove; a slide box 15 is provided on the surface of the connecting rack 14, and the slide box 15 is fixedly connected to the inner wall of the connecting groove, and the lower side of the surface of the connecting rack 14 is fixed A connecting slider 24 is connected, and the connecting slider 24 is slidably connected to the slide box 15. A pushing spring 25 is fixedly connected to the bottom of the connecting slider 24, and the other end of the pushing spring 25 is fixedly connected to the inner wall of the slide box 15; when installing the protective cover 6, the connecting plate 13 can be placed in the connecting groove, and the connecting rack 14 can be positioned by sliding cooperation between the limiting protrusion 23 and the limiting groove, so that the connecting rack 14 can be stably engaged with the connecting gear 17, and the circular block 16 is stopped by the locking structure to prevent the protective cover 6 from falling off. When installing the protective cover 6, the slide box 15 first touches the bottom of the connecting groove, and under the continuous pressing of the protective cover 6, the pushing spring 25 can be squeezed by the cooperation of the connecting slider 24 and the slide box 15. The pushing spring 25 can increase the tightness of the protective cover 6 and the incubator 3 during installation through its own elastic force. The surfaces of the protective cover 6 in contact with the incubator 3 are fixedly connected with sealing strips, which can further improve the tightness of the connection between the protective cover 6 and the incubator 3 to prevent leakage of the culture solution.

[0048] like Figure 6 , Figure 7 , Figure 8 and Fig. 9 As shown, the lock structure includes a card block 20 rotatably connected to the inner wall of the connecting groove, and the upper and lower ends of the card block 20 are respectively provided with card grooves 21, and the two sides corresponding to the circumferential surface of the circular block 16 are respectively fixedly connected with a matching claw 18 and a pushing claw 19, and the surface of the circular block 16 between the matching claw 18 and the pushing claw 19 is fixedly connected with a pushing block 22, and the inner end of the pushing claw 19 is provided with a protrusion 23 opened inwardly;

[0049] When the protective cover 6 is installed on the incubator 3, the circular block 16 is driven to rotate counterclockwise by the meshing of the connecting rack 14 and the connecting gear 17. When the protective cover 6 is pressed to the bottom, the circular block 16 rotates counterclockwise by the protrusion 23 of the pushing claw 19, so that the card slot 21 of the card block 20 corresponds to a corner of the pushing block 22. After the protective cover 6 is released, the connecting rack 14 moves under the push of the pushing spring 25, and the circular block 16 is reversed under the meshing of the connecting rack 14 and the connecting gear 17. When the push block 22 is in the position of the card slot 21, the position of the card slot 21 is protruding from the rotation track of the push block 22, that is, after the push block 22 is engaged with the card slot 21, the swing of the push block 22 and the rotation of the card block 20 will interfere with each other, so that the circular block 16 cannot continue to rotate under the push of the push spring 25, and the card block 20 cannot rotate either. After the circular block 16 cannot be reversed, the protective cover 6 cannot be separated from the incubator 3, and the push spring 25 can increase the pressure of the push block 22 on the card slot 21, so as to prevent the card block 20 from shaking under the action of external force.

[0050] When the protective cover 6 is removed, the protective cover 6 can be pressed again, and the pushing spring 25 can provide moving space for the movement of the connecting rack 14. When the connecting rack 14 drives the circular block 16 to rotate counterclockwise again by meshing with the connecting gear 17, one corner of the pushing block 22 is disengaged from the slot 21, and then the protrusion 23 of the pushing claw 19 pushes the block 20 to rotate again to make the slot 21 and the pushing block 22 misaligned, and then when the pushing spring 25 pushes the connecting rack 14 to move, the circular block 16 is driven to reverse under the meshing of the connecting rack 14 and the connecting gear 17. During the reversal of the circular block 16, the pushing block 22 pushes the block 20 to an inclined state and is placed in the space between the pushing block 22 and the matching claw 18 under the continued rotation of the circular block 16. Without the restriction of the block 20, the connecting rack 14 can move outward and disengage from the connecting gear 17, and then the protective cover 6 can be taken out;

[0051] By setting the locking structure, the convenience of installing and removing the protective cover 6 can be greatly improved. When installing the protective cover 6, the connecting plate 13 can be directly connected to the connecting groove and then the protective cover 6 can be pressed. After the protective cover 6 is pressed to the bottom, the protective cover 6 can be released. The protective cover 6 can be fixed on the incubator 3. When removing the protective cover 6, the protective cover 6 can be pressed again. After the protective cover 6 is released, the protective cover 6 can be taken out. The operation is simple and convenient. The outer end of the push block 22 away from the circular block 16 is an arc-shaped structure, and the left and right ends of the card block 20 are respectively provided with an arc surface structure that can fit with the outer end of the push block 22. After the circular block 16 is rotated to place the card block 20 in the space between the push block 22 and the mating claw 18, the arc surface structure of the end face of the card block 20 cooperates with the arc structure of the push block 22, so that the stability of the card block 20 can be improved.

[0052] Furthermore, in order to improve the stability of the pushing claw 19 and the matching claw 18, a vertically movable mounting slider 28 is slidably connected inside the connecting groove, a supporting spring 30 is fixedly connected to the bottom of the mounting slider 28, and the lower end of the supporting spring 30 is fixedly connected to the inner wall of the connecting groove, and a swing plate 26 is fixedly connected to the rear end of the pushing claw 19, and a matching groove 27 is opened on the surface of the swing plate 26 along the length direction, and a first pin shaft 29 is fixedly connected to the surface of the mounting slider 28, and the first pin shaft 29 is slidably matched with the inner side of the matching groove 27, and the first pin shaft 29 is fixedly connected to the inner side of the matching groove 27. The shaft 29 can make the installation slider 28 and the swing plate 26 interlocked by sliding cooperation with the matching groove 27, and then under the support of the support spring 30, when the connecting gear 17 is not under force, the swing plate 26 can be stably placed, and then the pushing claw 19 and the matching claw 18 can also be stably placed. When the connecting gear 17 rotates through the engagement of the connecting rack 14, the swing plate 26 can drive the installation slider 28 to move downward through the sliding cooperation with the first pin shaft 29 to compress the support spring 30 without affecting the swinging of the pushing claw 19 and the matching claw 18.

[0053] The surface of the liquid inlet pipe 9 is fixedly connected with a bearing wheel 31, and the circumferential surface of the bearing wheel 31 is rotatably connected with an annular plate 35. The surface of the liquid inlet pipe 9 is covered with a friction wheel 37, and the circumferential surfaces of the friction wheel 37 are respectively fixedly connected with a plurality of spring sheets 36, and the other ends of the spring sheets 36 are respectively fixedly connected with the annular plates 35. Under the pressure of the spring sheets 36, the friction wheel 37 is connected to the bearing wheel 31, and the friction wheel 37 and the bearing wheel 31 rotate through friction force. The surface of the friction wheel 37 is coaxially fixedly connected with a transmission tube 41, and the transmission tube 41 is sleeved on the surface of the liquid inlet pipe 9. The surface of the transmission tube 41 is slidably connected with a transmission sleeve 42, and the transmission sleeve 42 is rotatably connected to the vertical plate 2. A worm wheel 43 is fixedly connected to the surface of the transmission sleeve 42, and a worm 44 is meshed with one side of the worm wheel 43. The worm 44 is rotatably connected to the vertical plate 2, and a driving motor 45 is arranged on the lower side of the worm 44. The driving motor 45 It is fixedly connected to the vertical plate 2, and the output end on the upper side of the driving motor 45 is fixedly connected to the worm 44. When the culture vessel 3 is controlled to rotate, the driving motor 45 can be controlled to drive the worm 44 to rotate. When the worm 44 rotates, it can drive the transmission sleeve 42 to rotate through the engagement with the worm wheel 43. The inner wall of the transmission sleeve 42 is evenly and annularly fixedly connected with a strip protrusion, and the circumferential surface of the transmission tube 41 is evenly and annularly provided with a plurality of strip grooves. The strip protrusions and the strip grooves are slidably matched. When the transmission sleeve 42 rotates, the transmission tube 41 can also rotate. When the transmission tube 41 rotates, the liquid inlet tube 9 can be driven to rotate through the transmission of the friction wheel 37 and the bearing wheel 31, thereby driving the culture vessel 3 to rotate. The engagement of the worm 44 and the worm wheel 43 has a self-locking effect, which can greatly improve the stability of the culture vessel 3 during rotation, so that the culture vessel 3 can rotate stably without affecting the cell adhesion.

[0054] Further, in order to facilitate pouring out and replacing the culture solution inside the culture vessel 3, a linkage plate 38 is rotatably connected to the surface of the transmission tube 41, and a limit rod 40 is slidably connected to the bottom of the linkage plate 38. The limit rod 40 is fixedly connected to the vertical plate 2. The limit rod 40 is used to limit the linkage plate 38 so that the linkage plate 38 can move forward and backward stably. A second pin shaft 39 is fixedly connected to the right end of the linkage plate 38;

[0055] The surface of the vertical plate 2 is vertically provided with a mounting slide groove, and a linkage slide block 53 is slidably connected inside the mounting slide groove. The front and rear ends of the linkage slide block 53 are respectively fixedly connected to a handle 57 and a pressure rod 52. The rear end of the pressure rod 52 is fixedly connected to a guide plate 55. The lower end of the linkage slide block 53 is fixedly connected to a return spring 54. The lower end of the return spring 54 is fixedly connected to the lower end of the mounting slide groove. The surface of the guide plate 55 is provided with a guide groove 56 with a bottom end inclined toward the rear side. The second pin shaft 39 slides with the bottom end of the guide groove 56. When the guide plate 55 moves downward, the friction wheel 37 can be driven to move forward and disengage from the bearing wheel 31 through the sliding cooperation between the guide groove 56 and the second pin shaft 39. It is not only applicable to the friction wheel 37 and the bearing wheel 31 driven by friction transmission, but also applicable to the clutch components driven by meshing transmission. The surface of the shell 4 at the right end is provided with a liquid outlet 60, and the inner thread of the liquid outlet 60 is connected to the A blocking bolt 12 is connected, and the liquid outlet 60 is used to facilitate the outflow of the culture vessel 3. The blocking bolt 12 is used to block the liquid outlet 60. The blocking bolt 12 can be removed by rotating it, so that the culture solution can flow out through the liquid outlet 60. When in use, the handle 57 can be moved downward. When the handle 57 moves downward, not only can the reset spring 54 be squeezed by the linked slider 53, but the guide plate 55 can also be driven to move downward by the pressure rod 52. When the guide plate 55 moves downward, since the bottom of the guide groove 56 is inclined to the rear side, the second pin shaft 39 can be driven to move to the front side through the sliding cooperation between the guide groove 56 and the second pin shaft 39, and then when the guide plate 55 moves downward, the second pin shaft 39 is blocked by the vertical part of the guide groove 56. When the second pin shaft 39 moves to the front side, the friction wheel 37 is disengaged from the bearing wheel 31, and the culture vessel 3 can be rotated to face the liquid outlet 60, which is convenient for discharging the culture solution.

[0056] Furthermore, in order to facilitate the user to operate the culture vessel 3 to rotate and pour out the culture liquid, a ratchet 50 is fixedly connected to the front side of the liquid inlet tube 9, and a rotating disk 47 is rotatably connected to the surface of the ratchet 50. The surface of the rotating disk 47 is respectively rotatably connected to a plurality of ratchet claws 48 that mesh with the ratchet 50. The surfaces of the ratchet claws 48 are respectively fixedly connected to elastic sheets 49, and the other ends of the elastic sheets 49 are respectively fixedly connected to the rotating disk 47. Under the push of the elastic sheets 49, the ratchet claws 48 mesh with the ratchet 50. The surface of the rotating disk 47 is fixedly connected to a matching plate 51. The matching plate A vertical groove 58 and an inclined groove 59 are provided on the surface of 51, and the bottoms of the vertical groove 58 and the inclined groove 59 are connected. The pressure rod 52 is slidably matched at the upper end of the vertical groove 58. Under the sliding cooperation between the pressure rod 52 and the vertical groove 58, the rotating disk 47 cannot rotate. When the rotating disk 47 cannot rotate, the liquid inlet pipe 9 can rotate unidirectionally under the meshing of the pawl 48 and the ratchet 50 without affecting the rotating disk 47, so that the incubator 3 can rotate normally without affecting the pressure rod 52, and further without affecting the transmission between the friction wheel 37 and the bearing wheel 31. When the pressure rod 52 moves downward, it can pass through the inclined groove 5 The sliding cooperation of the pawl 48 and the ratchet 50 drives the rotating disk 47 to rotate. When the rotating disk 47 rotates, the liquid inlet pipe 9 is driven to rotate through the meshing of the pawl 48 and the ratchet 50. When the pressure rod 52 moves downward to pass through the vertical groove 58, the bearing wheel 31 and the friction wheel 37 can be disconnected under the sliding cooperation of the guide groove 56 and the second pin shaft 39. Then, after the pressure rod 52 slides to the inclined groove 59, it drives the matching plate 51 to swing, so that the rotating disk 47 rotates, and the meshing of the pawl 48 and the ratchet 50 drives the liquid inlet pipe 9 to rotate, thereby driving the incubator 3 to rotate so that the liquid outlet 60 faces downward. Pour out the culture solution; therefore, when the user needs to pour out the culture solution, the handle 57 can be directly controlled to move downward. When the handle 57 moves downward, the guide plate 55 can be driven downward by the pressure rod 52, and the friction wheel 37 is moved forward and disconnected from the bearing wheel 31 under the cooperation of the guide groove 56 and the second pin shaft 39. Then, in the process of the pressure rod 52 moving downward, the rotating disk 47 is driven to rotate through the cooperation with the inclined groove 59, and then when the rotating disk 47 rotates, the culture container 3 is driven to rotate through the engagement of the pawl 48 and the ratchet 50. The operation is simple and convenient.

[0057] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. An adherent cell culture reactor, comprising a bottom plate (1), the front side of which is fixedly connected to a vertically arranged upright plate (2), characterized in that: The rear side of the vertical plate (2) is rotatably connected to a culture vessel (3) arranged horizontally; the culture vessel (3) is composed of two symmetrically arranged shells (4) on the left and right and side plates (5) fixedly connected between the two shells (4) on the front and rear sides; the front side plate (5) is fixedly connected to and connected to a liquid inlet pipe (9); the liquid inlet pipe (9) is rotatably connected to the vertical plate (2); a driving structure for controlling the steady-speed rotation of the culture vessel (3) is installed on the lower side of the vertical plate (2); the output end of the driving structure is connected to the liquid inlet pipe (9); A partition plate (7) is fixedly connected between the two shells (4), and the space between the two shells (4) is divided into two upper and lower parts by the partition plate (7). Arc-shaped protective covers (6) are respectively arranged on the upper and lower sides of the partition plate (7). The two protective covers (6) are respectively detachably connected between the two shells (4). The inner walls of the two protective covers (6) are respectively fixedly connected with a culture bed (8), and the culture bed (8) is respectively in contact with the partition plate (7). A plurality of through holes (10) are respectively opened on the left and right sides of the surface of the partition plate (7).

2. The adherent cell culture reactor according to claim 1, characterized in that: The partition plate (7) is fixedly connected between the two shells (4) in a state where the left side is lower and the right side is higher.

3. The adherent cell culture reactor according to claim 1, characterized in that: The lower side of the through hole (10) on the left side of the partition plate (7) and the upper side of the through hole (10) on the right side of the partition plate (7) are respectively fixedly connected with an arc-shaped drainage tube (11).

4. The adherent cell culture reactor according to claim 1, characterized in that: The circumferential surface of the shell (4) is respectively fixedly connected with an annular circular plate (34), and the corresponding two sides of the circumferential surface of the circular plate (35) are respectively provided with connecting grooves inwardly, and the left and right sides of the upper end of the protective cover (6) are respectively fixedly connected with a connecting plate (13), and the front and rear ends of the connecting plate (13) are respectively provided with limiting protrusions (23), and the corresponding two sides of the inner wall of the connecting groove are respectively provided with limiting grooves, and the limiting protrusions (23) are slidably matched with the limiting grooves; the inside of the connecting groove is respectively rotatably connected with a circular block (16), and the middle part of the surface of the circular block (16) is respectively fixedly connected with a connecting gear (17), and one side of the connecting gear (17) is meshed with a connecting rack (14), and the upper end of the connecting rack (14) is The connecting rack (14) is fixedly connected to the bottom of the connecting plate (13). When the protective cover (6) is installed, the circular block (16) can be driven to rotate counterclockwise through the meshing of the connecting rack (14) and the connecting gear (17). A locking structure for fixing the circular block (16) is installed inside the connecting groove. A sliding groove box (15) is arranged on the surface of the connecting rack (14). The sliding groove box (15) is fixedly connected to the inner wall of the connecting groove. A connecting slider (24) is fixedly connected to the lower side of the surface of the connecting rack (14). The connecting slider (24) is slidably connected to the sliding groove box (15). A pushing spring (25) is fixedly connected to the bottom of the connecting slider (24). The other end of the pushing spring (25) is fixedly connected to the inner wall of the sliding groove box (15).

5. The adherent cell culture reactor according to claim 4, characterized in that: The locking structure comprises a clamping block (20) rotatably connected to the inner wall of the connecting groove, the clamping block (20) is provided with clamping grooves (21) at the upper and lower ends respectively, the corresponding two sides of the circumferential surface of the circular block (16) are respectively fixedly connected with a matching claw (18) and a pushing claw (19), the surface of the circular block (16) between the matching claw (18) and the pushing claw (19) is fixedly connected with a pushing block (22), and the inner end of the pushing claw (19) is provided with a protrusion (23) opened inwardly; When the circular block (16) rotates counterclockwise, the protrusion (23) of the pushing claw (19) can push the clamping block (20) to rotate to a vertical state, so that the clamping groove (21) of the clamping block (20) corresponds to a corner of the pushing block (22), and then when the pushing spring (25) pushes the connecting rack (14) to move, the circular block (16) is reversed under the meshing of the connecting rack (14) and the connecting gear (17). When the circular block (16) reverses, a corner of the pushing block (22) is stuck in the clamping groove (21), so that the circular block (16) cannot continue to rotate under the push of the pushing spring (25); When the connecting rack (14) engages with the connecting gear (17) again to drive the circular block (16) to rotate counterclockwise, one corner of the push block (22) is disengaged from the clamping groove (21), and then the protrusion (23) of the pushing claw (19) pushes the clamping block (20) to rotate again so that the clamping groove (21) and the pushing block (22) are misaligned, and then when the pushing spring (25) pushes the connecting rack (14) to move, the circular block (16) is driven to reverse under the meshing of the connecting rack (14) and the connecting gear (17). During the reverse rotation of the circular block (16), the clamping block (20) is pushed to an inclined state by the push block (22) and is placed in the space between the push block (22) and the matching claw (18) under the continuous rotation of the circular block (16).

6. The adherent cell culture reactor according to claim 5, characterized in that: The outer end of the push block (22) away from the circular block (16) is in an arc-shaped structure, and the left and right ends of the clamping block (20) are respectively provided with an arc surface structure that can fit with the outer end of the push block (22).

7. The adherent cell culture reactor according to claim 6, characterized in that: The interior of the connecting groove is slidably connected to a vertically movable mounting slide block (28); a supporting spring (30) is fixedly connected to the bottom of the mounting slide block (28); the lower end of the supporting spring (30) is fixedly connected to the inner wall of the connecting groove; a swing plate (26) is fixedly connected to the rear end of the pushing claw (19); a matching groove (27) is provided on the surface of the swing plate (26) along the length direction; a first pin shaft (29) is fixedly connected to the surface of the mounting slide block (28); the first pin shaft (29) is slidably matched on the inner side of the matching groove (27).

8. The adherent cell culture reactor according to claim 1, characterized in that: The surface of the liquid inlet pipe (9) is fixedly connected to a bearing wheel (31), and the circumferential surface of the bearing wheel (31) is rotatably connected to an annular plate (35). The surface of the liquid inlet pipe (9) is fitted with a friction wheel (37), and the circumferential surface of the friction wheel (37) is respectively fixedly connected to a plurality of spring sheets (36), and the other ends of the spring sheets (36) are respectively fixedly connected to the annular plates (35). Under the pressure of the spring sheets (36), the friction wheel (37) is connected to the bearing wheel (31), and the surface of the friction wheel (37) is coaxially fixedly connected to a transmission pipe (41). The tube (41) is sleeved on the surface of the liquid inlet tube (9), and a transmission sleeve (42) is slidably connected to the surface of the transmission tube (41), and the transmission sleeve (42) is rotationally connected to the vertical plate (2). A worm gear (43) is fixedly connected to the surface of the transmission sleeve (42), and a worm (44) is meshed on one side of the worm gear (43), and the worm (44) is rotationally connected to the vertical plate (2). A driving motor (45) is arranged on the lower side of the worm gear (44), and the driving motor (45) is fixedly connected to the vertical plate (2). The output end on the upper side of the driving motor (45) is fixedly connected to the worm gear (44).

9. The adherent cell culture reactor according to claim 8, characterized in that: The surface of the transmission tube (41) is rotatably connected to a linkage plate (38), the bottom of the linkage plate (38) is slidably connected to a limit rod (40), the limit rod (40) is fixedly connected to the vertical plate (2), and the right end of the linkage plate (38) is fixedly connected to a second pin shaft (39); The surface of the vertical plate (2) is vertically provided with an installation slide groove, and a linkage slide block (53) is slidably connected inside the installation slide groove. The front and rear ends of the linkage slide block (53) are respectively fixedly connected with a handle (57) and a pressure rod (52). The rear end of the pressure rod (52) is fixedly connected with a guide plate (55). The lower end of the linkage slide block (53) is fixedly connected with a return spring (54). The lower end of the return spring (54) is fixedly connected to the lower end of the installation slide groove. The surface of the guide plate (55) is provided with a guide groove (56) with a bottom end inclined toward the rear side. The second pin shaft (39) is slidably matched with the bottom end of the guide groove (56). When the guide plate (55) moves downward, the friction wheel (37) can be driven to move forward and disengage from the bearing wheel (31) through the sliding match between the guide groove (56) and the second pin shaft (39); the surface of the shell (4) at the right end is provided with a liquid outlet (60), and the liquid outlet (60) is internally threadedly connected with a blocking bolt (12).

10. The adherent cell culture reactor according to claim 9, characterized in that: A ratchet (50) is fixedly connected to the front side of the liquid inlet pipe (9), a rotating disk (47) is rotatably connected to the surface of the ratchet (50), a plurality of ratchet pawls (48) meshing with the ratchet (50) are rotatably connected to the surface of the rotating disk (47), elastic sheets (49) are fixedly connected to the surfaces of the ratchet pawls (48), the other ends of the elastic sheets (49) are fixedly connected to the rotating disk (47), the ratchet pawls (48) mesh with the ratchet (50) under the push of the elastic sheets (49), a matching plate (51) is fixedly connected to the surface of the rotating disk (47), a vertical groove (58) and a The inclined groove (59), the vertical groove (58) and the bottom of the inclined groove (59) are connected, and the pressure rod (52) is slidably matched with the upper end of the vertical groove (58). Under the sliding cooperation between the pressure rod (52) and the vertical groove (58), the rotating disk (47) cannot be rotated. When the rotating disk (47) cannot be rotated, the liquid inlet pipe (9) can be unidirectionally rotated without affecting the rotating disk (47) under the meshing of the pawl (48) and the ratchet (50). When the pressure rod (52) moves downward, the rotating disk (47) can be driven to rotate by the sliding cooperation with the inclined groove (59). When the rotating disk (47) rotates, the liquid inlet pipe (9) is driven to rotate by the meshing of the pawl (48) and the ratchet (50).

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