Dynamic in-situ monitoring method for corn root growth
By installing microroot canals from corn VE to V1 stages, and using root probe rods and scanning heads to obtain images in V4 stages, the problem of difficulty in installing corn root system monitoring in the black soil area in Northeast China before spring mechanical operation is achieved, and the corn root system is Acquisition of in-situ, continuous and accurate monitoring data.
Patent Information
- Application Number
- CN202411064000.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-05
- Publication Date
- 2024-12-06
AI Technical Summary
In the black soil area in the northeast, it is difficult to monitor the growth dynamics of corn root system before spring mechanical operations, especially the difficulty of micro-root canals being installed under the frozen layer, which affects data accuracy and installation difficulty.
Microroot canals were installed in corn VE to V1 phases, and 360° scan was performed using root probe rods and scanning heads in phase V4. The root image was obtained in combination with a micro camera, and the data was processed using root analysis software to ensure monitoring accuracy and reliability.
In-situ and continuous monitoring of the corn root system is realized, operations are simplified, and the accuracy and reliability of monitoring data are improved, and the planting conditions in the black soil area in Northeast China are adapted.
Smart Images
Figure CN119085483A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of plant root growth monitoring, and in particular to a method for dynamic in-situ monitoring of corn root growth. Background Art
[0002] The root system is the main organ for plants to absorb water and nutrients. Its distribution and morphology are closely related to the soil environment. Therefore, the root growth dynamics is not only an important indicator of plant growth and development, but also an important indicator of soil habitat. Real-time and on-site acquisition of root growth dynamics is of great scientific value for a comprehensive understanding of the root-soil relationship and guiding production practice.
[0003] Plant roots grow underground, and it is extremely difficult to obtain the root growth dynamics in situ and non-destructively. Since the development of root system research, there have been many monitoring methods, such as soil drilling method, soil column method, profile method, root chamber method, micro-root tube method, etc. Compared with other methods, the micro-root tube method is widely used in the study of plant root dynamics due to its advantages such as in situ and non-destructive. The black soil area in Northeast China is a one-year crop planting area with heavy texture, long frozen soil period, and short suitable operation time. In the past, micro-root tubes usually need to be pre-buried 3 to 6 months in advance to ensure that the micro-root tubes are closely integrated with the surrounding soil. If the micro-root tubes are installed after the autumn crops are harvested, it is easy to affect the mechanical operation in spring; if the micro-root tubes are installed after the corn is sown, the ground is often still frozen below 40 cm, which is extremely difficult to install. In addition, the location, angle, and number of micro-root tube installations will affect the quality and accuracy of root data.
[0004] Therefore, it is urgent to propose a method for in situ monitoring of the dynamics of corn root growth to solve the above technical problems. Summary of the invention
[0005] The purpose of the present invention is to solve the problem of the difficulty of installation caused by the common technology affecting the spring mechanical operation. A brief summary of the present invention is given below to provide a basic understanding of certain aspects of the present invention. It should be understood that this summary is not an exhaustive summary of the present invention. It is not intended to determine the key or important parts of the present invention, nor is it intended to limit the scope of the present invention.
[0006] The technical solution of the present invention:
[0007] A method for in-situ monitoring of the dynamics of corn root growth comprises the following steps:
[0008] Step 1: Install the micro-root tubes from the VE stage of corn (when the coleoptile emerges from the ground) to the V1 stage (when the first leaf is fully expanded);
[0009] Step 2: Use the soil drill that comes with the micro-root tube to pre-drill holes between the corn plants. Wrap the micro-root tube with black tape and seal the top of the micro-root tube with a cap. Then push the micro-root tube into the pre-drilled hole.
[0010] Step 3: Install cotton cloth on the root probe rod. When the fourth leaf of corn V4 is fully expanded, open the cover of the micro-root tube. Insert the root probe rod along the top of the micro-root tube to the bottom of the micro-root tube. Then place the root scanning head with a micro camera along the root probe rod to the bottom of the micro-root tube. Start the scanning head to scan the tube wall 360°. After the scanning is completed, use the probe rod to move and scan layer by layer until the ground, and mark the initial sampling position to obtain the root system image.
[0011] Step 4: Process the collected root images using root analysis software to generate root data for different soil layers or the entire soil profile based on research needs.
[0012] Preferably: in step 1, before installing the micro-root tubes, a soil drill is used to monitor the frozen soil layer between the corn plants on the ridge.
[0013] Preferably, in step 2, the angle between the pre-drilled hole and the ground is 45°, the depth of the pre-drilled hole from the ground is 85 cm, and the drilling is carried out layer by layer, and water is added during the advancement process.
[0014] Preferably, in step 2, the length of the micro-root tube is 1 m, and the black tape is wrapped around 15 to 20 cm from the upper end of the micro-root tube.
[0015] Preferably, when installing the micro-root tubes in step 2, at least three micro-root tubes are installed in the same row or the same treatment test field, the micro-root tubes are located between two corn plants, and adjacent micro-root tubes are spaced 80 cm apart.
[0016] Preferably, in step 3, the scanning head collects root system images every 10 days.
[0017] Preferably: each collected root image is processed using root analysis software to obtain information such as root length, root surface area, root volume, root diameter, number of nodes, number of branches, etc. in each image, and calculate root length density, root net growth rate and root turnover rate.
[0018] The present invention has the following beneficial effects:
[0019] The present invention realizes in-situ and continuous monitoring of corn root system, the operation process is simple, and the monitoring data is accurate and reliable:
[0020] The present invention improves the accuracy and reliability of monitoring results by installing micro-root canals at different positions. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1Schematic diagram of a method for in situ monitoring of maize root growth dynamics. DETAILED DESCRIPTION
[0022] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is described below by the specific embodiments shown in the accompanying drawings. However, it should be understood that these descriptions are only exemplary and are not intended to limit the scope of the present invention. In addition, in the following description, the description of well-known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.
[0023] Specific implementation method 1: Combination Figure 1 -Description of this embodiment: A method for in situ monitoring of the dynamic growth of corn root system in this embodiment comprises the following steps:
[0024] Step 1: Install the micro-root tubes from the VE stage of corn (when the coleoptile emerges from the ground) to the V1 stage (when the first leaf is fully expanded);
[0025] Step 2: Use the soil drill that comes with the micro-root tube to pre-drill holes between the corn plants. Wrap the micro-root tube with black tape and seal the top of the micro-root tube with a cap. Then push the micro-root tube into the pre-drilled hole.
[0026] Step 3: Install cotton cloth on the root probe rod. When the "fourth leaf is fully expanded" of corn V4 stage, open the cover on the micro-root tube, and insert the root probe rod along the top of the micro-root tube to the bottom of the micro-root tube. While wiping off the water vapor, move the auxiliary scanning head, and then place the root scanning head with a micro camera along the root probe rod to the bottom of the micro-root tube. Start the scanning head to scan the tube wall 360°. After the scanning is completed, use the probe rod to move and scan layer by layer until the ground, and mark the initial sampling position to obtain the root system image.
[0027] Step 4: Process the collected root images using root analysis software to generate root data for different soil layers or the entire soil profile based on research needs.
[0028] Specific implementation method 2: Combination Figure 1 The present embodiment is described as a method for in situ monitoring of the dynamic growth of corn root system. In step one, before installing the micro-root tube, a soil drill is used to monitor the frozen soil layer between the corn plants on the ridge. If the soil can be drilled to below 60 cm relatively easily, the installation can be carried out. If it is difficult to drill below 60 cm, the installation will be postponed.
[0029] Specific implementation method three: Combination Figure 1The present embodiment is described as a method for in situ monitoring of the dynamic growth of corn root system. In step 2, the angle between the pre-drilled hole and the ground is 45°, and the depth of the pre-drilled hole from the ground is 85 cm. The hole is drilled layer by layer, and water is added during the advancement process. On the one hand, it reduces resistance, and on the other hand, it is beneficial to maintain the shape of the drilled hole and reduce disturbance to the soil.
[0030] Specific implementation method four: Combination Figure 1 The present embodiment is described as a method for dynamic in situ monitoring of corn root growth. In step 2, the length of the micro-root tube is 1 m, and black tape is wrapped around 15 to 20 cm of the upper end of the micro-root tube to prevent light input from affecting root growth.
[0031] Specific implementation method five: Combination Figure 1 This embodiment describes a method for in situ monitoring of the dynamic growth of corn root systems. When installing the micro-root tubes in step 2, at least three micro-root tubes are installed in the same row or in the same treatment test field. The micro-root tubes are located between two corn plants, and adjacent micro-root tubes are spaced 80 cm apart. By installing the micro-root tubes in different positions, more information can be captured, thereby improving the accuracy and reliability of the monitoring results.
[0032] Specific implementation method six: Combination Figure 1 The present embodiment is described as a method for in-situ monitoring of the dynamics of corn root growth. In step three, the scanning head collects root images every 10 days to ensure that data are recorded at key stages of root growth, thereby more accurately reflecting its growth dynamics.
[0033] Specific implementation method seven: Combination Figure 1 This embodiment is described. This embodiment is a method for in-situ monitoring of the dynamic growth of corn root system. The root system images collected each time are processed using WinRHIZOTron MF 2019 root system analysis software to obtain information such as root length, root surface area, root volume, root diameter, number of nodes, number of branches, etc. in each image, and calculate the root length density, root net growth rate and root turnover rate. According to research needs, commonly used image processing software such as Photoshop, ImageJ, etc. can be used to splice, grayscale image conversion, binarization, etc., to generate root system data of different soil layers or the entire soil profile.
[0034] In order to verify the accuracy and reliability of the monitoring method, the method of the present invention was used to monitor the root system of corn under three different tillage methods, namely no-tillage, rotary tillage and strip tillage, and compared with the traditional soil drilling method. The soil drilling method was used to collect corn roots at the tasseling stage on July 18, 2022 and the maturity stage on September 14, 2022. Three plants were randomly selected from each tillage plot, and the aboveground part of the plant was removed. Then, a soil drill with a diameter of 8 cm was used to collect root samples in 4 layers of 15 cm each between two corn plants, i.e., 0-15 cm, 15-30 cm, 30-45 cm, and 45-60 cm. The samples were layered into a net bag with a label, and then rinsed with tap water, the old roots and impurities were manually removed, and the fresh fine roots were scanned with a root scanner. The comparison results of the root length density of corn obtained by the micro-root tube method and the soil drilling method are shown in Table 1, indicating that this method has good consistency with the soil drilling method.
[0035] Table 1
[0036]
[0037] It should be noted that in the above embodiments, as long as the technical solutions are not contradictory, they can be arranged and combined, and those skilled in the art can exhaust all possibilities based on the mathematical knowledge of arrangement and combination. Therefore, the present invention will no longer describe the technical solutions after arrangement and combination one by one, but it should be understood that the technical solutions after arrangement and combination have been disclosed by the present invention.
[0038] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A method for in situ monitoring of the dynamics of corn root growth, It is characterized in that The steps include: Step 1: Install the micro-root canal during the VE to V1 stage of corn; Step 2: Pre-drill holes between corn plants using a soil drill that matches the micro-root tube. Wrap the micro-root tube with black tape and seal the top of the micro-root tube with a cap. Then push the micro-root tube into the pre-drilled hole. Step 3: Install cotton cloth on the root probe rod, open the cover of the micro-root tube when the corn is in the V4 stage, insert the root probe rod along the top of the micro-root tube to the bottom of the micro-root tube, and then place the root scanning head with a micro camera along the root probe rod to the bottom of the micro-root tube, start the scanning head to scan the tube wall 360 degrees, and after the scanning is completed, use the probe rod to move and scan layer by layer until the ground, and mark the initial sampling position to obtain the root system image; Step 4: Process the collected root images using root analysis software to generate root data for different soil layers or the entire soil profile based on research needs.
2. A method for in-situ monitoring of corn root growth dynamics according to claim 1, Features: In the step 1, before installing the micro-root tubes, a soil drill is used to monitor the frozen soil layer between the corn plants on the ridge.
3. A method for in-situ monitoring of corn root growth dynamics according to claim 1, Features: In the step 2, the angle between the pre-drilled hole and the ground is 45°, and the depth of the pre-drilled hole from the ground is 85 cm. The pre-drilled hole is advanced layer by layer during the drilling process, and water is added during the advancement process.
4. A method for in-situ monitoring of the dynamics of corn root growth according to claim 1, Features: In the step 2, the length of the micro root tube is 1 m, and the black tape is wrapped around 15 to 20 cm of the upper end of the micro root tube.
5. A method for in-situ monitoring of the dynamics of corn root growth according to claim 1, Features: When installing the micro-root tubes in step 2, at least three micro-root tubes are installed in the same row or the same treatment test field. The micro-root tubes are located between two corn plants, and adjacent micro-root tubes are spaced 80 cm apart.
6. A method for in-situ monitoring of corn root growth dynamics according to claim 1, Features: In the step 3, the scanning head collects root system images every 10 days.
7. A method for in-situ monitoring of the dynamics of corn root growth according to claim 6, Features: The root images collected each time were processed using root analysis software to obtain information on root length, root surface area, root volume, root diameter, number of nodes, and number of branches in each image, and to calculate root length density, root net growth rate, and root turnover rate.