High internal phase emulsion as well as preparation and application thereof

Abstract

The invention provides a high internal phase emulsion as well as preparation and application thereof. The preparation method comprises the following steps: firstly, carrying out hydration, uniform mixing and enzymolysis on walnut protein and protein A, then uniformly mixing a double-protein zymolyte with naringenin dispersed in a dispersing agent, carrying out ultrasonic treatment, adding into water, finally uniformly mixing with coconut oil, and homogenizing to obtain the high internal phase emulsion. According to the present invention, the specific oil-water interface stabilizer naringenin is added to the double-protein zymolyte system, such that the walnut protein can maintain the good solubility and the good emulsifying property even in the pH 5-7 environment so as to significantly improve the physical stability of the high internal phase emulsion, and the daily chemical product prepared from the high internal phase emulsion has the good moisturizing effect, the application of the walnut protein in the field of daily chemical products is widened.

Description

Technical Field

[0001] The present invention belongs to the technical field of emulsion preparation, and more specifically, relates to a high internal phase emulsion and its preparation and application. Background Art

[0002] High internal phase emulsion is a type of emulsion with a dispersed phase volume fraction higher than 74.05%. Its dispersed phase droplets are separated by a continuous phase liquid film to form an irregular polyhedral structure. Compared with traditional emulsions, it exhibits better physical stability, and its unique oil-water interface structure can better protect bioactive substances and achieve controlled release and selective percutaneous absorption of bioactive substances. It is widely used in the field of daily chemicals.

[0003] At present, high internal phase emulsions usually use surfactants as emulsifiers to improve their own stability, but most surfactants are chemically synthesized and have limited nutritional value. Therefore, scholars have shifted the focus of emulsifier research and development to natural proteins with high yield, low price, sustainability and health benefits. Among them, walnut protein has attracted more widespread attention from scholars due to its good emulsification and high nutritional value.

[0004] However, walnut protein is greatly affected by pH. In the human skin environment with a pH of 5 to 7, its solubility and emulsification properties are poor, making it difficult to effectively stabilize high internal phase emulsions, which seriously limits its application in the daily chemical field. Summary of the invention

[0005] The present invention aims to provide a high internal phase emulsion and its preparation and application, which is obtained by hydrating, mixing and enzymolyzing walnut protein and protein A (sunflower seed protein / soybean protein), then mixing the double protein hydrolysate with naringenin dispersed in a dispersant, ultrasonicating, adding water, and finally mixing and homogenizing with coconut oil. The high internal phase emulsion can make the walnut protein maintain better solubility and emulsification even in an environment of pH 5 to 7, significantly improve its physical stability, and the daily chemical products prepared by the high internal phase emulsion also have better moisturizing effect, which broadens the application of walnut protein in the field of daily chemical products.

[0006] The first object of the present invention is to provide a method for preparing a high internal phase emulsion.

[0007] The second object of the present invention is to provide a high internal phase emulsion prepared by the above method.

[0008] The third object of the present invention is to provide the use of the high internal phase emulsion in the preparation of daily chemical products.

[0009] The fourth object of the present invention is to provide a daily chemical product.

[0010] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0011] The present invention provides a method for preparing a high internal phase emulsion, comprising the following steps:

[0012] S1. After hydrating walnut protein and protein A, mix them in a mass ratio of 3-7:3-7, and enzymolyze them with alkaline protease to obtain a double protease hydrolysate;

[0013] S2. dispersing naringenin in a dispersant, adding the mixture to the double protein hydrolysate, mixing, and ultrasonicating to obtain composite nanoparticles;

[0014] S3. adding the composite nanoparticles into water to obtain an aqueous phase;

[0015] S4. Mixing the aqueous phase and coconut oil and homogenizing to obtain a high internal phase emulsion;

[0016] Wherein, the protein A described in S1 is sunflower seed protein or soybean protein.

[0017] In the double protein hydrolysate system of the present invention, a specific oil-water interface stabilizer naringenin is added, so that the walnut protein can maintain better solubility and emulsification even in an environment of pH 5 to 7, thereby significantly improving the physical stability of the high internal phase emulsion, and the daily chemical products prepared by the high internal phase emulsion also have better moisturizing effect, which broadens the application of walnut protein in the field of daily chemical products. In addition, compared with the high internal phase emulsion prepared by other oil-water interface stabilizers, the high internal phase emulsion prepared by naringenin in the present invention has significantly better stability.

[0018] Preferably, the hydration in S1 is to hydrate the walnut protein and protein A simultaneously, or separately. The hydration can be completed in a manner commonly used in the art, such as stirring the walnut protein or protein A in water for 1 to 3 hours and then standing until fully hydrated. Optionally, the concentrations of the walnut protein and the protein A in water are independently selected from 2% (w / v) to 6% (w / v), preferably 4% (w / v). Optionally, the stirring rate is 90 to 110 rpm.

[0019] Preferably, the mass ratio of the walnut protein to protein A in S1 is 1:1.

[0020] Preferably, the temperature of the enzymatic hydrolysis in S1 is 50-55°C.

[0021] Preferably, the enzymatic hydrolysis time in S1 is 25 to 35 minutes.

[0022] Preferably, the pH of the enzymatic hydrolysis in S1 is 7.9 to 8.1, which can be adjusted using sodium hydroxide solution.

[0023] Preferably, the amount of alkaline protease in S1 is 0.8% (w / v) to 1.2% (w / v) of the mixed product.

[0024] Preferably, after the enzymatic hydrolysis in S1, enzyme inactivation and separation are also performed. Enzyme inactivation and separation can be performed in a manner commonly used in the art, such as: the enzyme inactivation is performed by heating; the separation is performed by centrifugation. Optionally, the enzyme inactivation by heating is performed by heating with boiling water for 7 to 9 minutes; the centrifugation is performed by centrifugation at 7800 to 8200 rpm for 8 to 12 minutes.

[0025] Preferably, the ratio of the total mass of the walnut protein and protein A after hydration in S1 to the mass of the naringenin in S2 is 3 to 5:1, and most preferably 4:1.

[0026] Preferably, the amount of naringenin added to the dispersant in S2 is 1% (w / v) to 2% (w / v), and most preferably 1% (w / v).

[0027] Preferably, the dispersant in S2 is an ethanol aqueous solution, such as an ethanol aqueous solution with a concentration of 75% (v / v) to 85% (v / v).

[0028] Preferably, the dispersion in S2 is achieved by stirring naringenin in a dispersant for 1 to 3 hours.

[0029] More preferably, the stirring rate is 90-110 rpm.

[0030] Preferably, the mixing in S2 is achieved by stirring for 0.5 to 1 hour.

[0031] More preferably, the stirring rate is 90-110 rpm.

[0032] Preferably, the power of the ultrasound in S2 is 400-500W, most preferably 450W.

[0033] Preferably, the temperature of the ultrasound in S2 is 20-30° C., most preferably 30° C. The temperature can be controlled by an ice bath.

[0034] Preferably, the ultrasonic time in S2 is 13 to 17 minutes, most preferably 15 minutes.

[0035] Preferably, after the ultrasound in S2, rotary evaporation is also performed.

[0036] More preferably, the rotary evaporation time is 15 to 30 minutes, most preferably 25 minutes.

[0037] More preferably, the rotary evaporation temperature is 45-55°C, most preferably 50°C.

[0038] More preferably, the rotation speed of the rotary evaporation is 115-125 rpm, most preferably 120 rpm.

[0039] Preferably, the concentration of the composite nanoparticles in water in S3 is 10 to 60 mg / mL.

[0040] Preferably, the volume ratio of the coconut oil to the aqueous phase in S4 is 75:23-25, most preferably 75:25.

[0041] Preferably, the homogenization speed in S4 is 12000-19000 rpm, most preferably 15000 rpm.

[0042] Preferably, the homogenization time in S4 is 1 to 3 minutes, most preferably 1.5 minutes.

[0043] When the high internal phase emulsion prepared by the above method is used to make a shower gel, it can not only significantly improve the rheology, viscosity and stability of the shower gel, but also significantly improve the anti-oxidation, moisturizing, cleaning and antibacterial effects of the shower gel. Therefore, the high internal phase emulsion prepared by the above method, the use of the high internal phase emulsion in the preparation of daily chemical products, and a daily chemical product containing the high internal phase emulsion should all be within the protection scope of the present invention.

[0044] Optionally, the daily chemical product is shower gel.

[0045] Preferably, the shower gel comprises the following components in parts by weight: 0.1-0.2 parts of sodium alginate, 0.1-0.2 parts of hydrogenated lecithin, 0.6-1.2 parts of corn modified starch, 1.5-7 parts of coconut oil foaming agent, 0.3-1.4 parts of D-panthenol, 0.4-1 parts of glycerol, 1.5-7 parts of the above-mentioned high internal phase emulsion, and 0.5-1 parts of sodium chloride. At this time, the pH value of the shower gel is 5-7, which is suitable for human skin.

[0046] More preferably, the shower gel further comprises water, and the amount of water used is sufficient to dissolve the sodium alginate.

[0047] Based on this, the present invention also provides a method for preparing the above-mentioned shower gel, namely: heating sodium alginate and hydrogenated lecithin to dissolve in water, then adding corn modified starch, coconut oil foaming agent, D-panthenol, glycerin, the above-mentioned high internal phase emulsion, and sodium chloride, mixing and homogenizing to obtain the shower gel.

[0048] Preferably, the heating temperature is 50-70°C.

[0049] Preferably, the mixing is achieved by magnetic stirring, such as stirring at 100 to 1500 rpm for 25 to 35 min.

[0050] Preferably, the homogenizing speed is 5000-10000 rpm, most preferably 6000 rpm.

[0051] Preferably, the homogenization time is 2 to 8 minutes, most preferably 5 minutes.

[0052] Optionally, the shower gel is a baby shower gel, wherein the term "baby" refers to babies aged 0 to 8 years old.

[0053] The present invention has the following beneficial effects:

[0054] 1. In the dual protein hydrolysate system of the present invention, the addition of a specific oil-water interface stabilizer, naringenin, can significantly improve the stability of the high internal phase emulsion.

[0055] 2. The high internal phase emulsion of the present invention is used to make shower gel, which can not only significantly improve the rheology, viscosity and stability of the shower gel, but also significantly improve the anti-oxidation, moisturizing, cleaning and antibacterial effects of the shower gel. In addition, the shower gel made with the high internal phase emulsion of the present invention as a raw material has a refreshing, non-greasy, tear-free feel, stable foam, easy to rinse, and natural ingredients, and has no toxic or irritating effects on the body, and is very suitable for use as a shower gel for infants and young children.

[0056] 3. The high internal phase emulsion of the present invention uses walnut protein, sunflower seed protein / soybean protein as raw materials, which broadens the application fields of these proteins and reduces the waste of plant-based protein resources.

[0057] 4. The preparation method of the high internal phase emulsion of the present invention is simple, consumes less energy, is highly safe, does not generate pollution, and is suitable for mass production. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 a is a perspective electron microscope image of composite nanoparticles. Figure 1 b is the scanning electron microscope image of the composite nanoparticles. Figure 1 c is the three-phase contact angle measurement results of composite nanoparticles.

[0059] Figure 2 A is the particle distribution diagram of S4 composite nanoparticles at the oil-water interface in Comparative Example 1, Figure 2 B is the particle distribution diagram of S4 composite nanoparticles at the oil-water interface in Comparative Example 3, Figure 2 C is the particle distribution diagram of S4 composite nanoparticles at the oil-water interface in Example 4.

[0060] Figure 3 a is an inverted photo of a high internal phase emulsion. Figure 3 b is a microscope image of a high internal phase emulsion. Figure 3 c is the change of transmittance of high internal phase emulsion with centrifugal displacement.

[0061] Figure 4 a is the high internal phase emulsion obtained in Example 1 after standing for 7 days in an environment of 25°C, Figure 4 b is the high internal phase emulsion obtained in Comparative Example 1 after standing at 25° C. for 7 days.

[0062] Figure 5 a is the high internal phase emulsion obtained in Example 1 after standing for 3 hours in an environment of 25°C, Figure 5 b is the high internal phase emulsion obtained in Comparative Example 2 after standing for 3 hours in an environment of 25°C, Figure 5 c is the high internal phase emulsion obtained in Comparative Example 3 after standing for 3 hours in an environment of 25°C, Figure 5 d is the high internal phase emulsion obtained in Comparative Example 4 after standing in an environment of 25°C for 3 hours.

[0063] Figure 6 a is the high internal phase emulsion obtained in Example 4 after standing in an environment of 25° C. for 24 hours, Figure 6 b is the high internal phase emulsion obtained in Comparative Example 3 after standing in an environment of 25° C. for 24 hours.

[0064] Figure 7 a is the high internal phase emulsion obtained in Comparative Example 1 after standing for 30 days in an environment of 25°C, Figure 7 b is the high internal phase emulsion obtained in Example 4 after standing at 25°C for 30 days, Figure 7 c is the high internal phase emulsion obtained in Comparative Example 3 after standing at 25°C for 30 days.

[0065] Figure 8 a is the high internal phase emulsion obtained in Example 1 after heating at 100°C for 30 minutes, Figure 8 b is the high internal phase emulsion obtained in Example 7 after heating at 100°C for 30 minutes.

[0066] Fig. 9 The results of rheological properties.

[0067] Fig.10 The viscosity is measured.

[0068] Fig.11 The results of the antioxidant properties.

[0069] Fig.12 The results are for the moisturizing and cleansing performance tests.

[0070] Fig.13 a is the antibacterial performance test result of shower gel 1, Fig.13 b is the antibacterial performance test result of shower gel 2, Fig.13 c is the antibacterial performance test result of shower gel 3, Fig.13 d is the test result of antibacterial performance of commercially available shower gel. DETAILED DESCRIPTION

[0071] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0072] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0073] Example 1 Preparation of high internal phase emulsion

[0074] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0075] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0076] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0077] S4. naringenin was added to 80% (v / v) ethanol aqueous solution at an addition amount of 1% (w / v), magnetically stirred at 100 rpm for 2 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin was 4:1), magnetically stirred at 100 rpm for 1 h, ultrasonicated at 450 W and 30 ° C for 15 min, and then rotary evaporated at 50 ° C and 120 rpm for 25 min to obtain composite nanoparticles;

[0078] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0079] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0080] Example 2 Preparation of high internal phase emulsion

[0081] S1. Add walnut protein to water (the concentration of walnut protein in water is 2% (w / v)), stir magnetically at 110 rpm for 1 h, and let stand until fully hydrated;

[0082] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 2% (w / v)), stir magnetically at 110 rpm for 1 h, and let stand until fully hydrated;

[0083] S3. The S1 product and the S2 product were mixed at a mass ratio of 3:7, the pH was adjusted to 7.9 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 0.8% (w / v) of the mixed product), and the mixture was shaken in a 55°C water bath for 25 min, heated in boiling water for 7 min, and centrifuged at 7800 rpm for 12 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0084] S4. 1% (w / v) naringenin was added to 75% (v / v) ethanol aqueous solution, magnetically stirred at 110 rpm for 1 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin was 3:1), magnetically stirred at 110 rpm for 0.5 h, ultrasonicated at 500 W and 20° C. for 13 min, and then rotary evaporated at 55° C. and 125 rpm for 15 min to obtain composite nanoparticles;

[0085] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0086] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 19000 rpm for 1 min to obtain a high internal phase emulsion.

[0087] Example 3 Preparation of high internal phase emulsion

[0088] S1. Add walnut protein to water (the concentration of walnut protein in water is 6% (w / v)), stir magnetically at 90 rpm for 3 h, and let stand until fully hydrated;

[0089] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 6% (w / v)), stir magnetically at 90 rpm for 3 h, and let stand until fully hydrated;

[0090] S3. The S1 product and the S2 product were mixed at a mass ratio of 7:3, the pH was adjusted to 8.1 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1.2% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 35 minutes, heated in boiling water for 9 minutes, and centrifuged at 8200 rpm for 10 minutes. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0091] S4. 2% (w / v) naringenin was added to 85% (v / v) ethanol aqueous solution, magnetically stirred at 90 rpm for 3 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin was 5:1), magnetically stirred at 90 rpm for 1 h, ultrasonicated at 400 W and 30 ° C for 17 min, and then rotary evaporated at 45 ° C and 115 rpm for 30 min to obtain composite nanoparticles;

[0092] S5. The composite nanoparticles are added to water (so that the concentration of the composite nanoparticles in water is 10 mg / mL) to obtain an aqueous phase;

[0093] S6. Mix the coconut oil and water phase in a volume ratio of 75:23, and homogenize at 12000 rpm for 3 min to obtain a high internal phase emulsion.

[0094] Example 4 Preparation of high internal phase emulsion

[0095] The same as Example 1, except that in S4, the amount of naringenin added is 2% (w / v). That is, the specific embodiment of this embodiment is:

[0096] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0097] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0098] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0099] S4. Add naringenin in an amount of 2% (w / v) to 80% (v / v) ethanol aqueous solution, stir magnetically at 100 rpm for 2 h, then add to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin is 4:1), stir magnetically at 100 rpm for 1 h, ultrasonicate at 450 W and 30 ° C for 15 min, and then rotary evaporate at 50 ° C and 120 rpm for 25 min to obtain composite nanoparticles;

[0100] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0101] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0102] Example 5 Preparation of high internal phase emulsion

[0103] The same as Example 1, except that in S4, the ultrasonic power is 150W, and the rotary evaporation time is 30 minutes; in S6, the homogenization is carried out at 19000 rpm for 1 minute. That is, the specific embodiment of this embodiment is:

[0104] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0105] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0106] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0107] S4. naringenin was added to 80% (v / v) ethanol aqueous solution at an addition amount of 1% (w / v), magnetically stirred at 100 rpm for 2 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin was 4:1), magnetically stirred at 100 rpm for 1 h, ultrasonicated at 150 W and 30 ° C for 15 min, and then rotary evaporated at 50 ° C and 120 rpm for 30 min to obtain composite nanoparticles;

[0108] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0109] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 19000 rpm for 1 min to obtain a high internal phase emulsion.

[0110] Example 6 Preparation of high internal phase emulsion

[0111] The same as Example 1, except that in S4, the ultrasonic power is 600W, and the rotary evaporation time is 15 minutes; in S6, the homogenization is carried out at 12000 rpm for 3 minutes. That is, the specific embodiment of this embodiment is:

[0112] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0113] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0114] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0115] S4. naringenin was added to 80% (v / v) ethanol aqueous solution at an addition amount of 1% (w / v), magnetically stirred at 100 rpm for 2 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin was 4:1), magnetically stirred at 100 rpm for 1 h, ultrasonicated at 600 W and 30° C. for 15 min, and then rotary evaporated at 50° C. and 120 rpm for 15 min to obtain composite nanoparticles;

[0116] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0117] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 12000 rpm for 3 min to obtain a high internal phase emulsion.

[0118] Example 7 Preparation of high internal phase emulsion

[0119] The same as Example 1, except that the sunflower seed protein is replaced by soybean protein. That is, the specific embodiment of this example is:

[0120] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0121] S2. Add soy protein to water (the concentration of soy protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0122] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0123] S4. naringenin was added to 80% (v / v) ethanol aqueous solution at an addition amount of 1% (w / v), magnetically stirred at 100 rpm for 2 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of naringenin was 4:1), magnetically stirred at 100 rpm for 1 h, ultrasonicated at 450 W and 30 ° C for 15 min, and then rotary evaporated at 50 ° C and 120 rpm for 25 min to obtain composite nanoparticles;

[0124] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0125] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0126] Comparative Example 1

[0127] The same as Example 1, except that naringenin was not added, that is, the double protein hydrolysate obtained in S3 was directly subjected to the operation of S5 in Example 1; and in order to form nanoparticles, an ultrasonic treatment of S4 was also performed after S5. That is, this comparative example is specifically:

[0128] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0129] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0130] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0131] S4. Add the double protein hydrolysate to water (so that the concentration of the double protein hydrolysate in water is 60 mg / mL), and ultrasonicate at 450 W and 30 ° C for 15 min to obtain composite nanoparticles (aqueous phase);

[0132] S5. Coconut oil and composite nanoparticles (aqueous phase) were mixed in a volume ratio of 75:25, and homogenized at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0133] Comparative Example 2

[0134] The same as Example 1, except that in S4, naringenin is replaced by catechin, and the amount of catechin added is 0.5% (w / v); and because catechin is a water-soluble component, water is used as a dispersant, and the rotary evaporation operation to remove ethanol is not performed. That is, this comparative example is specifically:

[0135] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0136] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0137] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0138] S4. 0.5% (w / v) catechin was added to water, magnetically stirred at 100 rpm for 2 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to catechin mass was 4:1), magnetically stirred at 100 rpm for 1 h, and ultrasonicated at 450 W and 30 ° C for 15 min to obtain composite nanoparticles;

[0139] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0140] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0141] Comparative Example 3

[0142] The difference is the same as Example 1, in S4, naringenin is replaced by catechin, and the amount of catechin added is 1% (w / v); and because catechin is a water-soluble component, water is used as a dispersant, and the rotary evaporation operation to remove ethanol is not performed. That is, this comparative example is specifically:

[0143] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0144] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0145] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0146] S4. catechin was added to water at an addition amount of 1% (w / v), magnetically stirred at 100 rpm for 2 h, then added to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of catechin was 4:1), magnetically stirred at 100 rpm for 1 h, and ultrasonicated at 450 W and 30 ° C for 15 min to obtain composite nanoparticles;

[0147] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0148] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0149] Comparative Example 4

[0150] The same as Example 1, except that in S4, naringenin is replaced by catechin, and the amount of catechin added is 2% (w / v); and because catechin is a water-soluble component, water is used as a dispersant, and the rotary evaporation operation to remove ethanol is not performed. That is, this comparative example is specifically:

[0151] S1. Add walnut protein to water (the concentration of walnut protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0152] S2. Add sunflower seed protein to water (the concentration of sunflower seed protein in water is 4% (w / v)), stir magnetically at 100 rpm for 3 h, and let stand until fully hydrated;

[0153] S3. The S1 product and the S2 product were mixed at a mass ratio of 1:1, the pH was adjusted to 8.0 with 1M NaOH solution, alkaline protease was added (the amount of alkaline protease was 1% (w / v) of the mixed product), and the mixture was shaken in a 50°C water bath for 30 min, heated in boiling water for 8 min, and centrifuged at 8000 rpm for 10 min. The supernatant obtained by centrifugation was the double protein hydrolysate;

[0154] S4. Add catechins to water at an addition amount of 2% (w / v), stir magnetically at 100 rpm for 2 h, then add to the double protein hydrolysate (so that the total mass ratio of S1 product and S2 product to the mass ratio of catechins is 4:1), stir magnetically at 100 rpm for 1 h, and ultrasonicate at 450 W and 30 ° C for 15 min to obtain composite nanoparticles;

[0155] S5. The composite nanoparticles were added to water (so that the concentration of the composite nanoparticles in water was 60 mg / mL) to obtain an aqueous phase;

[0156] S6. Mix the coconut oil and water phase in a volume ratio of 75:25, and homogenize at 15000 rpm for 1.5 min to obtain a high internal phase emulsion.

[0157] Test Example 1

[0158] 1. Characterization of composite nanoparticles

[0159] The microstructure of the S4 composite nanoparticles in Example 1 was observed and analyzed using a transmission electron microscope (TEM) and a scanning electron microscope (SEM), and the three-phase contact angle was measured using a contact angle meter (Dataphysics OCA20). The results are as follows: Figure 1 As shown. Among them, Figure 1 a is a perspective electron microscope image of composite nanoparticles. Figure 1 b is the scanning electron microscope image of the composite nanoparticles. Figure 1 c is the three-phase contact angle measurement result of the composite nanoparticles. It can be seen that the S4 composite nanoparticles of Example 1 are cross-linked under hydrogen bonding and hydrophobic effects to form nanoparticles with small particle size (~320nm), nearly spherical shape, and high interface wettability (~84.5°).

[0160] The particle distribution of S4 composite nanoparticles at the oil-water interface in Example 4, Comparative Example 1, and Comparative Example 3 was observed and analyzed using a scanning electron microscope. Figure 2 As shown. Among them, Figure 2 A is the particle distribution diagram of S4 composite nanoparticles at the oil-water interface in Comparative Example 1, Figure 2B is the particle distribution diagram of S4 composite nanoparticles at the oil-water interface in Comparative Example 3, Figure 2 C is the particle distribution diagram of S4 composite nanoparticles at the oil-water interface in Example 4.

[0161] It can be seen that the S4 composite nanoparticles in Example 4 form a multi-layer cross-linked network structure at the oil-water interface, which is more conducive to forming a stable high internal phase emulsion, indicating that only by adding an oil-water interface stabilizer and only by using the specific oil-water interface stabilizer naringenin of the present invention can the stability of the high internal phase emulsion be significantly improved.

[0162] 2. Characterization of high internal phase emulsion

[0163] The high internal phase emulsion obtained in Example 1 was inverted, and the result was as follows: Figure 3 As shown in a. It can be seen that the high internal phase emulsion obtained in Example 1 does not flow, that is, it has good stability.

[0164] The high internal phase emulsion obtained in Example 1 was observed under an optical microscope. Figure 3 As shown in b, it can be seen that the droplets of the high internal phase emulsion obtained in Example 1 are uniform in size and tightly packed together, indicating that it has good physical stability.

[0165] The high internal phase emulsion obtained in Example 1 was placed in a stability analyzer (iFUGE111) and centrifuged at 4000 rpm for 3600 s to observe the change in the transmittance of the high internal phase emulsion with centrifugal displacement. The results are as follows: Figure 3 As shown in c. It can be seen that the high internal phase emulsion obtained in Example 1 has good centrifugal stability.

[0166] In addition, the present invention also uses the same method to test the high internal phase emulsions obtained in Examples 2 to 3, and the test results show that they are basically consistent with the test results of the high internal phase emulsion obtained in Example 1.

[0167] 3. Stability test of high internal phase emulsion

[0168] (1) Stand at 25℃ for 7 days

[0169] The high internal phase emulsions obtained in Example 1 and Comparative Example 1 were placed in an environment of 25°C for 7 days, and their stratification was observed. The high internal phase emulsion obtained in Example 1 without stratification was inverted for observation. The results are as follows: Figure 4 As shown. Among them, Figure 4 a is the high internal phase emulsion obtained in Example 1 after standing for 7 days in an environment of 25°C, Figure 4 b is the high internal phase emulsion obtained in Comparative Example 1 after standing at 25° C. for 7 days.

[0170] It can be seen that the stability of Example 1 is significantly better than that of Comparative Example 1, indicating that only by adding an oil-water interface stabilizer can the stability of the high internal phase emulsion of the present invention be significantly improved.

[0171] (2) Stand at 25℃ for 3h

[0172] The high internal phase emulsions obtained in Example 1 and Comparative Examples 2 to 4 were placed in an environment of 25° C. for 3 hours, and their stratification was observed. The high internal phase emulsion obtained in Example 1 without stratification was inverted and observed. The results are as follows: Figure 5 As shown. Among them, Figure 5 a is the high internal phase emulsion obtained in Example 1 after standing for 3 hours in an environment of 25°C, Figure 5 b is the high internal phase emulsion obtained in Comparative Example 2 after standing for 3 hours in an environment of 25°C, Figure 5 c is the high internal phase emulsion obtained in Comparative Example 3 after standing for 3 hours in an environment of 25°C, Figure 5 d is the high internal phase emulsion obtained in Comparative Example 4 after standing in an environment of 25°C for 3 hours.

[0173] It can be seen that the stability of Example 1 is significantly better than that of Comparative Examples 2 to 4, indicating that only by using the specific oil-water interface stabilizer naringenin of the present invention can the stability of the high internal phase emulsion be significantly improved.

[0174] (3) Stand at 25℃ for 24h

[0175] The high internal phase emulsions obtained in Example 4 and Comparative Example 3 were placed in an environment of 25°C for 24 hours, and their stratification was observed. The high internal phase emulsion obtained in Example 4 without stratification was inverted for observation. The results are as follows: Figure 6 As shown. Among them, Figure 6 a is the high internal phase emulsion obtained in Example 4 after standing in an environment of 25° C. for 24 hours, Figure 6 b is the high internal phase emulsion obtained in Comparative Example 3 after standing in an environment of 25° C. for 24 hours.

[0176] It can be seen that the stability of Example 4 is significantly better than that of Comparative Example 3, indicating that only by using the specific oil-water interface stabilizer naringenin of the present invention can the stability of the high internal phase emulsion be significantly improved.

[0177] (4) Stand at 25℃ for 30 days

[0178] The high internal phase emulsions obtained in Example 4, Comparative Example 1 and Comparative Example 3 were placed in an environment of 25°C for 30 days, and their stratification was observed. The results are as follows: Figure 7 As shown. Among them, Figure 7 a is the high internal phase emulsion obtained in Comparative Example 1 after standing for 30 days in an environment of 25°C, Figure 7 b is the high internal phase emulsion obtained in Example 4 after standing at 25°C for 30 days, Figure 7 c is the high internal phase emulsion obtained in Comparative Example 3 after standing at 25°C for 30 days.

[0179] It can be seen that the stability of Example 4 is significantly better than that of Comparative Example 1 and Comparative Example 3, indicating that only by adding an oil-water interface stabilizer and only by using the specific oil-water interface stabilizer naringenin of the present invention can the stability of the high internal phase emulsion be significantly improved.

[0180] (5) Heating at 100°C for 30 min

[0181] The high internal phase emulsions obtained in Example 1 and Example 7 were heated at 100°C for 30 minutes respectively, and their stratification was observed. The results are as follows: Figure 8 As shown. Among them, Figure 8 a is the high internal phase emulsion obtained in Example 1 after heating at 100°C for 30 minutes, Figure 8 b is the high internal phase emulsion obtained in Example 7 after heating at 100°C for 30 minutes.

[0182] It can be seen that the high internal phase emulsions obtained in Example 1 and Example 7 can remain stable after heating without oil droplets precipitating, indicating that the high internal phase emulsion of the present invention has good stability.

[0183] Application Example 1

[0184] 1. Preparation of shower gel 1

[0185] 0.1 g of sodium alginate and 0.1 g of hydrogenated lecithin were dissolved in 60° C. deionized water, and then 0.6 g of corn modified starch (food grade), 3.5 g of coconut oil foaming agent, 0.7 g of D-panthenol, 0.5 g of glycerol, 3.5 g of the high internal phase emulsion obtained in Example 1, and 0.5 g of sodium chloride were added. The mixture was stirred magnetically at 550 rpm for 30 min and homogenized at 6000 rpm for 5 min to obtain shower gel 1.

[0186] 2. Preparation of Shower Gel 2

[0187] 0.1 g of sodium alginate and 0.1 g of hydrogenated lecithin were dissolved in 60° C. deionized water, and then 0.6 g of corn modified starch (food grade), 3.5 g of coconut oil foaming agent, 0.7 g of D-panthenol, 0.5 g of glycerol, 3.5 g of the high internal phase emulsion obtained in Example 6, and 0.5 g of sodium chloride were added. The mixture was stirred magnetically at 550 rpm for 30 min and homogenized at 6000 rpm for 5 min to obtain shower gel 2.

[0188] 3. Preparation of shower gel 3

[0189] 0.1 g of sodium alginate and 0.1 g of hydrogenated lecithin were dissolved in 60° C. deionized water, and then 0.6 g of corn modified starch (food grade), 3.5 g of coconut oil foaming agent, 0.7 g of D-panthenol, 0.5 g of glycerol, 3.5 g of the high internal phase emulsion obtained in Example 5, and 0.5 g of sodium chloride were added. The mixture was stirred magnetically at 550 rpm for 30 min and homogenized at 6000 rpm for 5 min to obtain shower gel 3.

[0190] 4. Preparation of shower gel 4

[0191] 0.1 g of sodium alginate and 0.1 g of hydrogenated lecithin were dissolved in 60° C. deionized water, and then 0.6 g of corn modified starch (food grade), 5 g of coconut oil foaming agent, 0.9 g of D-panthenol, 0.8 g of glycerol, 1.5 g of the high internal phase emulsion obtained in Example 1, and 0.5 g of sodium chloride were added. The mixture was stirred magnetically at 550 rpm for 30 min and homogenized at 10000 rpm for 2 min to obtain shower gel 4.

[0192] 5. Preparation of Shower Gel 5

[0193] 0.1 g of sodium alginate and 0.1 g of hydrogenated lecithin were dissolved in 60° C. deionized water, and then 0.6 g of corn modified starch (food grade), 1.5 g of coconut oil foaming agent, 0.3 g of D-panthenol, 0.4 g of glycerol, 6.0 g of the high internal phase emulsion obtained in Example 1, and 0.5 g of sodium chloride were added. The mixture was stirred magnetically at 550 rpm for 30 min and homogenized at 5000 rpm for 8 min to obtain shower gel 5.

[0194] 6. Preparation of shower gel 6

[0195] 0.1 g of sodium alginate and 0.1 g of hydrogenated lecithin were dissolved in 60° C. deionized water, and then 0.6 g of corn modified starch (food grade), 3.5 g of coconut oil foaming agent, 0.7 g of D-panthenol, 0.5 g of glycerol, 3.5 g of the high internal phase emulsion obtained in Example 1, and 0.5 g of sodium chloride were added. The mixture was stirred magnetically at 550 rpm for 30 min and homogenized at 8000 rpm for 7 min to obtain shower gel 6.

[0196] 7. Rheological properties

[0197] The rheological behavior of shower gels 1 to 6 was measured using a Haake rheometer, with a parallel plate of 35 mm in diameter and a gap of 1 mm. The frequency sweep mode was used, the frequency sweep range was 0.1 to 100 Hz, and the strain was 0.01%. The results are shown in Fig. 9 shown.

[0198] It can be seen that shower gels 1 to 3 have good rheological properties, and shower gel 1 has the best rheological properties, indicating that the shower gel prepared by using the high internal phase emulsion of the present invention has good rheological properties.

[0199] The present invention also uses the same method to perform rheological analysis on shower gels 4 to 6. The results show that they are basically consistent with the analysis conclusion of shower gel 1, and all have good rheological properties.

[0200] 8. Viscosity

[0201] The viscosities of shower gels 1 to 6 were analyzed using a Brookfield DV-III Ultra viscometer. The results are shown in the figure below. Fig.10 shown.

[0202] It can be seen that shower gels 1 to 3 have relatively high viscosities, and shower gel 1 has the highest viscosity, indicating that the shower gel prepared by using the high internal phase emulsion of the present invention has relatively high physical stability.

[0203] In addition, the present invention also uses the same method to analyze the viscosity of a commercially available shower gel (Raccoon Chamomile Shower Gel), and it is found that the viscosity of shower gel 1 is close to that of the commercially available shower gel.

[0204] 9. Antioxidant Properties

[0205] Sample solution: Dilute the volume of shower gel 1 to 3 by 10 times with distilled water to obtain sample solution.

[0206] Sample group: Take 2 mL of 0.2 mM DPPH ethanol solution and mix with 2 mL of sample solution, shake with a vortex shaker for 1 min, and then place in the dark for 30 min, and measure the absorbance value A at 517 nm. 样品 .

[0207] Blank group 1: Mix 2 mL of ethanol with 2 mL of sample solution, vortex for 1 min, and place in a dark place for 30 min. Measure the absorbance value A at 517 nm. 空白样1 .

[0208] Blank group 2: Mix 2 mL of 0.2 mM DPPH ethanol solution with 2 mL of distilled water, vortex for 1 min, and place in the dark for 30 min. Measure the absorbance at 517 nm. 空白样2 .

[0209] According to "clearance rate (%) = [1-(A 样品 -A 空白样1 ) / A 空白样2 ]×100%” to calculate the DPPH free radical scavenging rate of shower gels 1 to 3. The results are as follows Fig.11 shown.

[0210] It can be seen that shower gels 1 to 3 all have high DPPH free radical scavenging rates, and shower gel 1 has the highest rate, indicating that the shower gel prepared by using the high internal phase emulsion of the present invention has high antioxidant properties.

[0211] 10. Moisturizing and Cleansing Performance

[0212] (1) Determination of moisturizing properties

[0213] Twelve children (3 to 8 years old) with similar arm skin conditions were selected and divided into three groups. Areas of the same size were demarcated on the inner side of their left forearms as test areas. After measuring the skin moisture content A0 with a skin moisture tester, 0.1 mL of shower gel 1 to 3 diluted with water at a volume ratio of 1:1 was applied to the test area. After 30 seconds, it was rinsed with 25-30℃ distilled water, dried with a paper towel, and left to sit for 1 hour. Then, the skin moisture content A1 in the test area was measured with a skin moisture tester. Each test was repeated 5 times, and the average of all the measurement results in the same group was taken. Finally, the skin moisture increase rate was calculated according to "skin moisture increase rate (%) = (A1-A0) / A0×100%", and the moisturizing performance of shower gels 1 to 3 was evaluated. The results are as follows Fig.12 shown.

[0214] It can be seen that shower gels 1 to 3 all have a high skin moisture increase rate, and shower gel 1 has the highest rate, indicating that the high internal phase emulsion of the present invention can make walnut protein maintain better solubility and emulsification even in an environment of pH 5 to 7, significantly improving its own physical stability and the moisturizing effect of the daily chemical products prepared by the high internal phase emulsion. In addition, it can be observed that shower gels 1 to 3 have a refreshing, non-greasy, tear-free skin feel, stable foam, easy to rinse, and natural ingredients, no toxic or irritating effects on the body, and are very suitable for use as infant shower gels.

[0215] (2) Cleaning performance measurement

[0216] 3g of shower gels 1 to 3 were added to 1000mL of distilled water respectively, pre-stirred and dispersed for 2min, then a piece of protein-stained cloth was put in (the whiteness value A0 was measured by a whiteness meter at this time), and the cloth was left to stand for 10min. The cloth was taken out, rinsed with clean water and hung to dry, and the whiteness value A1 was measured by a whiteness meter at this time. Finally, the detergency was calculated according to "detergency (%) = (A1-A0) / A0×100%" to evaluate the cleaning performance of shower gels 1 to 3. Three parallel samples were set for each shower gel, and the results were averaged. Fig.12 shown.

[0217] It can be seen that shower gels 1 to 3 all have relatively high detergency, and shower gel 1 has the highest detergency, indicating that the shower gel prepared by using the high internal phase emulsion of the present invention has relatively high cleaning performance.

[0218] 11. Antibacterial performance

[0219] According to the method of GB / T 7918.2-1987 "Standard Method for Microbiological Examination of Cosmetics - Determination of Total Bacteria", the degree of bacterial contamination of shower gels 1 to 3 was determined. The results are as follows: Fig.13 As shown. Among them, Fig.13 a is the antibacterial performance test result of shower gel 1, Fig.13 b is the antibacterial performance test result of shower gel 2, Fig.13 c is the antibacterial performance test result of shower gel 3, Fig.13 d is the test result of antibacterial performance of commercially available shower gel.

[0220] It can be seen that the ability of shower gels 1 to 3 to inhibit the growth of microorganisms in the air is significantly better than that of the commercially available shower gel (Raccoon Chamomile Shower Gel), and all have good antibacterial properties, and the antibacterial property of shower gel 1 is the best, indicating that the shower gel prepared using the high internal phase emulsion of the present invention has higher antibacterial properties.

[0221] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A method for preparing a high internal phase emulsion, characterized in that: The steps include: S1. After hydrating walnut protein and protein A, mix them in a mass ratio of 3-7:3-7, and enzymolyze them with alkaline protease to obtain a double protease hydrolysate; S2. dispersing naringenin in a dispersant, adding the mixture to the double protein hydrolysate, mixing, and ultrasonicating to obtain composite nanoparticles; S3. adding the composite nanoparticles into water to obtain an aqueous phase; S4. Mixing the aqueous phase and coconut oil and homogenizing to obtain a high internal phase emulsion; Wherein, the protein A described in S1 is sunflower seed protein or soybean protein.

2. The preparation method according to claim 1, characterized in that: The ratio of the total mass of the walnut protein and protein A after hydration in S1 to the mass of the naringenin in S2 is 3 to 5:

1.

3. The preparation method according to claim 1, characterized in that: The power of the ultrasound in S2 is 400-500W.

4. The preparation method according to claim 1, characterized in that: The ultrasound time in S2 is 13 to 17 minutes.

5. The preparation method according to claim 1, characterized in that: S3 The concentration of the composite nanoparticles in water is 10 to 60 mg / mL.

6. The preparation method according to claim 1, characterized in that: The volume ratio of coconut oil to water phase in S4 is 75:23-25.

7. The preparation method according to claim 1, characterized in that: The homogenizing speed in S4 is 12000-19000 rpm.

8. The high internal phase emulsion prepared by the method according to any one of claims 1 to 7.

9. Use of the high internal phase emulsion according to claim 8 in the preparation of daily chemical products.

10. A daily chemical product, characterized in that: Comprising the high internal phase emulsion according to claim 8.

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