A method for tracing the sources of carbon and nitrogen elements based on isotope labeling

Abstract

The present invention discloses a method for tracing the source of carbon and nitrogen elements based on isotope labeling, and relates to the technical field of element tracing. The method comprises: data acquisition: setting the initial temperature, obtaining the initial temperature item, obtaining the movement speed of the labeled isotope under the initial temperature item, and obtaining the initial speed item; stage division: dividing the reaction process into the first stage and the second stage, respectively setting the speed threshold for the first stage and the second stage, obtaining the first stage threshold item and the second stage threshold item, increasing the reaction speed in the first stage, and reducing the reaction speed in the second stage, limiting the maximum speed of the labeled isotope in the first stage and the minimum speed in the second stage. The present invention uses an adjustment method to adjust the real-time temperature based on the detection interval item, obtains the movement speed of the labeled isotope, obtains the real-time speed item, and judges the real-time speed item of the labeled isotope, thereby obtaining the optimal reaction temperature of the labeled isotope.

Description

Technical Field

[0001] The present invention relates to the technical field of element tracing, and in particular to a method for tracing the sources of carbon and nitrogen elements based on isotope labeling. Background Art

[0002] Carbon and nitrogen are two important chemical elements that play a key role in nature. Carbon is one of the basic building blocks of life and is the basis of all organic molecules such as proteins, fats, carbohydrates and nucleic acids. Plants fix carbon dioxide through photosynthesis and are the main source of energy in the ecosystem. Nitrogen mainly exists in molecular form in nature, accounting for about 78% of the Earth's atmosphere. It also exists in the form of ammonia, nitrates and organic nitrogen compounds. Carbon and nitrogen are closely related in the ecosystem. Plants absorb carbon dioxide during photosynthesis and need nitrogen to synthesize proteins and nucleic acids. The concentration and circulation status of these two elements will affect each other, thereby affecting the growth of plants and the ecological balance. Tracking the sources of carbon and nitrogen has many important scientific and practical significances. Tracking the sources of carbon and nitrogen can help identify and quantify the sources of pollutants, such as agricultural fertilization, industrial wastewater discharge, etc.

[0003] Patent publication number CN109932290A is a particle counting method based on streaming image motion target tracking. According to the position information of the tracking target, the Kalman filter method is used to predict the position of the tracking target in each frame image, and the position of the moving target detected in the subsequent frame image of the video is compared. The relative distance between the two positions is used as the basis for judging whether it belongs to the corresponding tracking target. Compared with the static motion target counting technology such as FlowCam and MFI, it avoids repeated counting and avoids the influence of impurity background and sample uniformity on the results, thereby improving the accuracy of the analysis process. The result is closer to the actual value. In addition, the particle counting method of the dynamic tracking technology has been used for the first time in microbial analysis and water environment analysis technology.

[0004] When the above-mentioned and similar technical solutions track the sources of carbon and nitrogen elements through isotopes, when the experiment is not conducted in the laboratory and a stable reaction temperature cannot be provided, the temperature differences in different regions will have different impacts on the experimental process. When the temperature is too high or too low, it will affect the reaction of the elements, and at different reaction stages, the optimal temperature required for the reaction process is not fixed. As the reaction proceeds, the reaction rate will decrease or speed up accordingly, thereby causing certain interference to the tracking process of the staff. When the reaction rate decreases, it takes more time to complete the tracking of the sources of carbon and nitrogen elements, and when the reaction rate is faster, it is easy to fail to obtain the precise process information of the reaction. Summary of the invention

[0005] The purpose of the present invention is to provide a method for tracing the sources of carbon and nitrogen elements based on isotope labeling to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: a method for tracing the source of carbon and nitrogen elements based on isotope labeling, comprising:

[0007] Data acquisition: set the initial temperature to obtain the initial temperature term, obtain the movement speed of the labeled isotope under the initial temperature term, and obtain the initial speed term;

[0008] Stage division: the reaction process is divided into the first stage and the second stage, and the speed thresholds are set for the first stage and the second stage respectively, so as to obtain the first stage threshold item and the second stage threshold item. The first stage increases the reaction speed, and the second stage decreases the reaction speed, so as to limit the maximum speed of the labeled isotope in the first stage and the minimum speed in the second stage;

[0009] Temperature regulation: The initial temperature item is adjusted by the adjustment method to obtain the movement speed of the labeled isotope and the real-time speed item. Based on the continuous acquisition of the real-time speed item, the optimal reaction temperature of the labeled isotope is obtained to ensure that the labeled isotope always achieves the best reaction;

[0010] The adjustment method comprises:

[0011] Step 1: setting the detection interval, setting the detection interval, obtaining the detection interval item, continuously acquiring the movement speed of the labeled isotope according to the detection interval item, obtaining the real-time speed item, and then obtaining the real-time speed set, the real-time speed set including at least one real-time speed item;

[0012] Step 2: Peak acquisition: based on the real-time velocity set, the velocity peak value of the labeled isotope is obtained to obtain the velocity peak item, and the target temperature corresponding to the velocity peak item is obtained to obtain the temperature change item;

[0013] Step 3: stage confirmation, confirm the stage of the reaction. When the reaction is in the first stage, compare the speed peak item with the first stage threshold item. When the speed peak item does not reach the first stage threshold item, the target temperature corresponding to the speed peak item is used as the temperature change item. When the speed peak item reaches the first stage threshold item, the target temperature corresponding to the first stage threshold item is used as the temperature change item. When the reaction is in the second stage, the target temperature corresponding to the second stage threshold item is used as the temperature change item.

[0014] Step 4: Temperature change. Adjust the temperature based on the temperature change item to ensure the stability of the movement of the labeled isotope and the intensity of the reaction.

[0015] Furthermore, the method for setting the detection interval includes:

[0016] S1: interval adjustment, setting the change threshold and the positive and negative growth ratio, obtaining the difference in movement speed of two adjacent labeled isotopes in the real-time speed set, obtaining a speed difference item, and then obtaining a speed difference set, which includes at least one speed difference item;

[0017] S2: Difference judgment, judgment is made based on the speed difference set. When there is no speed difference item that reaches the change threshold in the speed difference set, the detection interval item is extended based on the positive growth ratio. When there is a speed difference item that reaches the change threshold in the speed difference set, the detection interval item is shortened based on the negative growth ratio.

[0018] Furthermore, the method for obtaining the temperature change item includes:

[0019] N1: feature point acquisition, based on the speed difference item, positive and negative judgment is performed. When the speed difference item is negative, the two real-time speed items constituting the speed difference item are respectively set as the first speed feature point and the second speed feature point;

[0020] N2: The temperature change item is determined, and the target temperature corresponding to the first speed characteristic point is used as the temperature change item.

[0021] Furthermore, the method for obtaining the temperature change item also includes: setting an extended threshold value, setting the extended threshold value, in step N1, when the speed difference item is a negative number, obtaining the speed difference item within the target extended threshold value again based on the extended threshold value, and performing a positive and negative judgment again, when the speed difference items within the target extended threshold value are all negative numbers, selecting the first negative speed difference item as the target difference item, and repeating steps N1-N2, when there is a positive number in the speed difference item within the target extended threshold value, setting the first negative speed difference item as the comparison difference item, repeating steps N1-N2, using the obtained temperature change item as the comparison temperature item, repeating steps N1-N2 again, and then obtaining the extended temperature item, and comparing it with the comparison temperature item, when the extended temperature item is greater than the comparison temperature item, using the extended temperature item as the temperature change item, otherwise using the comparison temperature item as the temperature change item.

[0022] Furthermore, the stage division method includes:

[0023] M1: Change point setting, set the detection time of the labeled isotope, take the detection time as the change point, and get the first range and the second range. The first range is from the start of the reaction to the change point, and the second range is from the change point to the end of the reaction;

[0024] M2: stage correspondence, based on the first range and the second range corresponding to the first stage and the second stage respectively, the first range corresponds to the first stage, and the second range corresponds to the second stage.

[0025] Furthermore, the method for stage confirmation includes: stage judgment, confirming whether the stage of the reaction has reached a change point, and then performing stage confirmation. When the stage of the reaction has not reached the change point, confirming that the stage of the reaction is the first stage, when the stage of the reaction reaches the change point, confirming that the stage of the reaction is the second stage.

[0026] Furthermore, the method for obtaining the threshold item of the first stage includes:

[0027] X1: Image acquisition, obtaining the state images of labeled isotopes at different temperatures to obtain a state image set;

[0028] X2: critical point determination, based on the state image set, determine the highest critical temperature of the target marker isotope and obtain the critical temperature term;

[0029] X3: Protection threshold setting. Set the protection threshold. Based on the protection threshold and the critical speed corresponding to the critical temperature item, set the decrease range of the critical speed item to obtain the first stage threshold item.

[0030] Furthermore, the method for obtaining the second-stage threshold item includes: determining the lowest critical temperature of the target marker isotope based on the state image set, and setting the speed increase amplitude based on the protection threshold and the critical speed corresponding to the lowest critical temperature to obtain the second-stage threshold item.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The carbon and nitrogen element source tracing method based on isotope labeling obtains an initial temperature item and a detection interval item respectively by setting an initial temperature and setting a detection interval. Under the premise of the initial temperature item, the movement speed of the labeled isotope is obtained to obtain an initial speed item. Through an adjustment method, the real-time temperature is adjusted based on the detection interval item to obtain the movement speed of the labeled isotope and obtain a real-time speed item. The real-time speed item of the labeled isotope is judged to obtain the optimal reaction temperature of the labeled isotope. According to continuous judgment of the real-time speed item, the real-time temperature is continuously adjusted to ensure that the labeled isotope always reaches the highest reaction speed when the state is normal.

[0033] At the same time, the entire reaction stage is divided into two stages. The first stage increases the reaction intensity of the labeled isotope, and the second stage reduces the reaction intensity of the labeled isotope. The first stage increases the reaction rate in order to increase the mixing and growth rate of the labeled isotope, and the second stage reduces the reaction rate in order to better detect the labeled isotope and reduce the activity of the labeled isotope, which is more conducive to detection. While adjusting the temperature by adjusting the method, the movement speed of the labeled isotope at different temperatures is obtained, so that the optimal reaction temperature of the labeled isotope can be obtained. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the relationship between data acquisition, stage division and temperature regulation of the present invention;

[0035] Figure 2 It is a schematic flow chart of the adjustment method of the present invention;

[0036] Figure 3 A schematic diagram of a first speed characteristic point and a second speed characteristic point of the present invention;

[0037] Figure 4 A schematic diagram of a method for obtaining a temperature change item of the present invention;

[0038] Figure 5 It is a schematic diagram of the relationship between the comparison temperature item and the expansion temperature item of the present invention. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] At the end of the reaction, it is necessary to maintain the stability of carbon and nitrogen elements to improve the accuracy of source tracking. At this time, it is necessary to appropriately reduce the reaction intensity of carbon and nitrogen elements. Therefore, during the entire reaction process, different degrees of reaction speed need to be provided to achieve different required effects. The reaction speed is adjusted by controlling the temperature, and it is also necessary to consider whether there is damage to the labeled isotope. The technical solution provided by the present application sets the initial temperature and the detection interval during the entire reaction process, and obtains the initial temperature item and the detection interval item respectively. Under the premise of the initial temperature item, the movement speed of the labeled isotope is obtained to obtain the initial speed item. Through the adjustment method, the real-time temperature is adjusted based on the detection interval item to obtain the movement speed of the labeled isotope and obtain the real-time speed item. By judging the real-time speed item of the labeled isotope, the optimal reaction temperature of the labeled isotope is obtained. According to the continuous judgment of the real-time speed item, the real-time temperature is continuously adjusted to ensure that the labeled isotope always reaches the highest reaction speed when the state is normal, and the entire reaction stage is divided into two stages. The first stage increases the reaction intensity of the labeled isotope, and the second stage reduces the reaction intensity of the labeled isotope, so that the labeled isotope can be observed more carefully.

[0041] like Figure 1-Figure 3As shown, the present invention provides a technical solution: a method for tracing the source of carbon and nitrogen elements based on isotope labeling, comprising: data acquisition: setting an initial temperature to obtain an initial temperature item, obtaining the movement speed of the labeled isotope under the initial temperature item, and obtaining an initial speed item; stage division: dividing the reaction process into a first stage and a second stage, setting speed thresholds for the first stage and the second stage respectively, obtaining a first stage threshold item and a second stage threshold item, increasing the reaction speed in the first stage, and reducing the reaction speed in the second stage, limiting the maximum speed of the labeled isotope in the first stage and the minimum speed in the second stage; temperature adjustment: adjusting the initial temperature item by an adjustment method, obtaining the movement speed of the labeled isotope, and obtaining a real-time speed item, and according to the continuous acquisition of the real-time speed item, obtaining the optimal reaction temperature of the labeled isotope, ensuring that the labeled isotope reaches the optimal reaction at all times; the adjustment method comprises: step one: detection interval setting, setting the detection interval, obtaining the detection interval item, according to The detection interval item continuously obtains the movement speed of the labeled isotope to obtain the real-time speed item, and then obtains the real-time speed set, which includes at least one real-time speed item; step two: peak acquisition, based on the real-time speed set, obtain the speed peak of the labeled isotope to obtain the speed peak item, and obtain the target temperature corresponding to the speed peak item to obtain the temperature change item; step three: stage confirmation, confirm the stage of the reaction, when the reaction is in the first stage, compare the speed peak item with the first stage threshold item, when the speed peak item does not reach the first stage threshold item, the target temperature corresponding to the speed peak item is used as the temperature change item, when the speed peak item reaches the first stage threshold item, the target temperature corresponding to the first stage threshold item is used as the temperature change item, when the reaction is in the second stage, the target temperature corresponding to the second stage threshold item is used as the temperature change item; step four: temperature change, adjust the temperature based on the temperature change item to ensure the movement stability of the labeled isotope and the reaction intensity.

[0042] It should be noted that the initial temperature is set at 15°C to obtain the movement speed of the labeled isotope at 15°C, and the reaction process is divided into the first stage and the second stage. The first stage is the development stage, and the second stage is the detection stage. The first stage increases the reaction speed in order to increase the mixing and growth rate of the labeled isotope. The second stage reduces the reaction speed in order to better detect the labeled isotope and reduce the activity of the labeled isotope, which is more conducive to detection. While adjusting the temperature through the adjustment method, the movement speed of the labeled isotope at different temperatures is obtained, so that the optimal reaction temperature of the labeled isotope can be obtained.

[0043] It should be noted that the detection interval is set to 2s and the temperature adjustment ratio is set to 1°C. According to the detection interval and the temperature adjustment ratio, the movement speed of the labeled isotope is continuously obtained, and the speed peak of the labeled isotope is obtained, and the temperature change item is obtained according to the temperature corresponding to the speed peak.

[0044] In a specific implementation process, a certain labeled isotope starts from an initial temperature of 15°C and increases the temperature by 1°C every 2s. At the initial temperature, the movement speed of the target labeled isotope is 5cm / s. After 8s, the temperature rises to 19°C, at which time the movement speed of the target labeled isotope gradually increases to 12cm / s. After another 2s, the temperature rises to 20°C, at which time the movement speed of the target labeled isotope is 11cm / s, and the optimal reaction temperature of the target labeled isotope is 19°C. After a period of reaction, the movement speed of the target labeled isotope stabilizes at 12cm / s. After another 2s, the movement speed of the target labeled isotope decreases to 11cm / s. At this time, the reaction temperature is reduced to 18°C ​​according to the temperature adjustment ratio. At this time, the movement speed of the target labeled isotope increases to 12cm / s. After another 2s, the temperature rises to 19°C, at which time the movement speed of the target labeled isotope decreases again to 11cm / s, and the optimal reaction temperature of the target labeled isotope is 18°C.

[0045] The method for setting the detection interval includes: S1: interval adjustment, setting a change threshold and a positive and negative growth ratio, obtaining the difference in movement speed of two adjacent labeled isotopes in the real-time speed set, obtaining a speed difference item, and then obtaining a speed difference set, the speed difference set including at least one speed difference item; S2: difference judgment, judging based on the speed difference set, when there is no speed difference item reaching the change threshold in the speed difference set, extending the detection interval item based on the positive growth ratio, and when there is a speed difference item reaching the change threshold in the speed difference set, shortening the detection interval item based on the negative growth ratio.

[0046] It should be noted that the set change threshold is 0.5 cm / s, and the positive and negative growth ratio is ±200%. By obtaining the difference in movement speed of two adjacent labeled isotopes in the real-time speed set, when the speed difference does not reach the change threshold, the detection interval item is extended based on the positive growth ratio. When the speed difference reaches the change threshold, the detection interval item is shortened based on the negative growth ratio.

[0047] In the specific implementation process, a certain labeled isotope starts from an initial temperature of 15°C. At the initial temperature, the movement speed of the target labeled isotope is 5 cm / s. After 2 seconds, the movement speed increases to 6 cm / s. At this time, the speed difference is 1 cm / s, which does not reach the change threshold. The detection interval item is extended based on the positive growth ratio. At this time, the detection interval is 4 seconds. After 4 seconds, the movement speed increases to 9 cm / s. At this time, the speed difference is 3 cm / s, which does not reach the change threshold. The detection interval item is extended based on the positive growth ratio. At this time, the detection interval is 8 seconds. After 8 seconds, the movement speed increases to 9.3 cm / s. At this time, the movement difference is 0.3 m / s, which reaches the change threshold. At this time, the detection interval item is shortened based on the negative growth ratio.

[0048] like Figure 3 As shown, the method for obtaining the temperature change item includes: N1: characteristic point acquisition, positive and negative judgment is made based on the speed difference item. When the speed difference item is a negative number, the two real-time speed items constituting the speed difference item are respectively set as the first speed characteristic point and the second speed characteristic point; N2: temperature change item determination, with the target temperature corresponding to the first speed characteristic point as the temperature change item.

[0049] It should be noted that when the speed difference is a negative number, the speed detected later will be smaller than the speed detected last time. The two detected speeds are set as the first speed characteristic point and the second speed characteristic point respectively, and the target temperature corresponding to the first speed characteristic point is used as the temperature change item.

[0050] In the specific implementation process, a certain labeled isotope starts from an initial temperature of 15°C. At the initial temperature, the movement speed of the target labeled isotope is 5 cm / s. At the tenth detection, the movement speed is 12 cm / s and the temperature is 19°C. After another 2s, the movement speed is 11 cm / s and the temperature is 20°C. The speeds of the tenth detection and the eleventh detection are set as the first speed characteristic point and the second speed characteristic point, respectively, and the target temperature corresponding to the first speed characteristic point is used as the temperature change item. At this time, the target temperature corresponding to the tenth detection is used as the temperature change item, that is, 19°C is used as the temperature change item.

[0051] like Figure 4-5As shown, the method for obtaining the temperature change item also includes: setting an extended threshold value, setting the extended threshold value, in step N1, when the speed difference item is a negative number, obtaining the speed difference item within the target extended threshold value again based on the extended threshold value, and performing a positive and negative judgment again, when the speed difference items within the target extended threshold value are all negative numbers, selecting the first negative speed difference item as the target difference item, and repeating steps N1-N2, when there is a positive number in the speed difference item within the target extended threshold value, setting the first negative speed difference item as the comparison difference item, repeating steps N1-N2, using the obtained temperature change item as the comparison temperature item, repeating steps N1-N2 again, and then obtaining the extended temperature item, and comparing it with the comparison temperature item, when the extended temperature item is greater than the comparison temperature item, using the extended temperature item as the temperature change item, otherwise using the comparison temperature item as the temperature change item.

[0052] It should be noted that the set extension threshold is the number of detections of 3. When the speed difference item is negative, the speed difference item within the target extension threshold is obtained again based on the extension threshold, and the positive and negative judgment is performed again. When the speed difference items within the number of detections of 3 are all negative, the first negative speed difference item is selected as the target difference item, and steps N1-N2 are repeated.

[0053] In the specific implementation process, a certain labeled isotope starts from an initial temperature of 15°C. At the initial temperature, the movement speed of the target labeled isotope is 5 cm / s. At the tenth detection, the movement speed is 12 cm / s and the temperature is 18°C. After another 2s, the movement speed is 11 cm / s and the temperature is 19°C. At this time, the speed difference is a negative number. Based on the expansion threshold, the speed difference item within the target expansion threshold is obtained again. For the twelfth detection, the movement speed is 10 cm / s and the temperature is 20°C. For the thirteenth detection, the movement speed is 9 cm / s and the temperature is 21°C. For the fourteenth detection, the movement speed is 8 cm / s and the temperature is 22°C. At this time, the speed difference between the tenth and eleventh times is used as the target difference, and the target temperature corresponding to the tenth detection is obtained as the temperature change item, that is, 18°C ​​is used as the temperature change item.

[0054] It should be noted that when there is a positive speed difference item within the target expansion threshold, the first negative speed difference item is set as the comparison difference item, and steps N1-N2 are repeated. The obtained temperature change item is used as the comparison temperature item, and steps N1-N2 are repeated again to obtain the extended temperature item, which is compared with the comparison temperature item. When the extended temperature item is greater than the comparison temperature item, the extended temperature item is used as the temperature change item, otherwise the comparison temperature item is used as the temperature change item.

[0055] In the specific implementation process, a certain labeled isotope starts from an initial temperature of 15°C. At the initial temperature, the movement speed of the target labeled isotope is 5 cm / s. At the tenth detection, the movement speed is 12 cm / s and the temperature is 18°C. After another 2s, the movement speed is 11 cm / s and the temperature is 19°C. At this time, the speed difference is a negative number. Based on the extended threshold, the speed difference item within the target extended threshold is obtained again. In the twelfth detection, the movement speed is 12 cm / s and the temperature is 20°C. In the thirteenth detection, the movement speed is 13 cm / s and the temperature is 21°C. In the fourteenth detection, the movement speed is 12 cm / s and the temperature is 22°C. At this time, the comparison temperature item is 18°C ​​and the movement speed is 12 cm / s, while the extended temperature item is 21°C and the movement speed is 13 cm / s. The extended temperature item is greater than the comparison temperature item. The extended temperature item is used as the temperature change item, and the speed difference between the thirteenth and fourteenth times is used as the target difference. The target temperature corresponding to the thirteenth detection is obtained as the temperature change item, that is, 21°C is used as the temperature change item.

[0056] The method of stage division includes: M1: change point setting, setting the detection time of the labeled isotope, taking the detection time as the change point, and obtaining the first range and the second range, the first range is from the start of the reaction to the change point, and the second range is from the change point to the end of the reaction; M2: stage correspondence, based on the first range and the second range, respectively corresponding to the first stage and the second stage, the first range corresponds to the first stage, and the second range corresponds to the second stage.

[0057] It should be noted that the detection time of the labeled isotope as the change point is the reaction time of the target labeled isotope. When the reaction time is reached, the labeled isotope can be detected. The range before the detection point is the first stage, and the range after the detection point is the second stage.

[0058] The method of stage confirmation includes: stage judgment, confirming whether the stage of the reaction has reached the change point, and then performing stage confirmation. When the stage of the reaction has not reached the change point, confirming that the stage of the reaction is the first stage, when the stage of the reaction reaches the change point, confirming that the stage of the reaction is the second stage.

[0059] The method for obtaining the threshold item of the first stage includes: X1: image acquisition, obtaining the state images of the labeled isotope at different temperatures to obtain a state image set; X2: critical point determination, based on the state image set, determining the highest critical temperature of the target labeled isotope to obtain the critical temperature item; X3: protection threshold setting, setting the protection threshold, based on the protection threshold and the critical speed corresponding to the critical temperature item, setting the decrease range of the critical speed item to obtain the threshold item of the first stage.

[0060] It should be noted that the state image set includes state images of labeled isotopes at different temperatures. When the highest critical temperature is reached, the state image of the labeled isotope obtained at this time is on the verge of degradation. The set protection threshold is 10%. The critical temperature is lowered based on the protection threshold, and the first-stage threshold item is obtained.

[0061] The method for obtaining the second stage threshold item includes: determining the lowest critical temperature of the target marker isotope based on the state image set, and setting the speed increase amplitude based on the protection threshold and the critical speed corresponding to the lowest critical temperature to obtain the second stage threshold item.

[0062] It should be noted that when the lowest critical temperature is reached, the state image of the labeled isotope obtained at this time is that no reaction is generated, the activity is inhibited, and the protection threshold is set to 10%. The critical temperature is increased based on the protection threshold, and the second stage threshold item is obtained.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is limited by the attached embodiments and their equivalents.

Claims

1. A method for tracing the source of carbon and nitrogen elements based on isotope labeling, characterized in that: include: Data acquisition: set the initial temperature to obtain the initial temperature term, obtain the movement speed of the labeled isotope under the initial temperature term, and obtain the initial speed term; Stage division: the reaction process is divided into the first stage and the second stage, and the speed thresholds are set for the first stage and the second stage respectively, so as to obtain the first stage threshold item and the second stage threshold item. The first stage increases the reaction speed, and the second stage decreases the reaction speed, so as to limit the maximum speed of the labeled isotope in the first stage and the minimum speed in the second stage; Temperature regulation: The initial temperature item is adjusted by the adjustment method to obtain the movement speed of the labeled isotope and the real-time speed item. Based on the continuous acquisition of the real-time speed item, the optimal reaction temperature of the labeled isotope is obtained to ensure that the labeled isotope always achieves the best reaction; The adjustment method comprises: Step 1: setting the detection interval, setting the detection interval and the temperature adjustment ratio, obtaining the detection interval item and the temperature adjustment item, continuously acquiring the movement speed of the labeled isotope according to the detection interval item and the temperature adjustment item, obtaining the real-time speed item, and then obtaining the real-time speed set, which includes at least one real-time speed item; Step 2: Peak acquisition: based on the real-time velocity set, the velocity peak value of the labeled isotope is obtained to obtain the velocity peak item, and the target temperature corresponding to the velocity peak item is obtained to obtain the temperature change item; Step 3: stage confirmation, confirm the stage of the reaction. When the reaction is in the first stage, compare the speed peak item with the first stage threshold item. When the speed peak item does not reach the first stage threshold item, the target temperature corresponding to the speed peak item is used as the temperature change item. When the speed peak item reaches the first stage threshold item, the target temperature corresponding to the first stage threshold item is used as the temperature change item. When the reaction is in the second stage, the target temperature corresponding to the second stage threshold item is used as the temperature change item. Step 4: Temperature change. Adjust the temperature based on the temperature change item to ensure the stability of the movement of the labeled isotope and the intensity of the reaction.

2. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 1, characterized in that: The method for setting the detection interval includes: S1: interval adjustment, setting the change threshold and the positive and negative growth ratio, obtaining the difference in movement speed of two adjacent labeled isotopes in the real-time speed set, obtaining a speed difference item, and then obtaining a speed difference set, which includes at least one speed difference item; S2: Difference judgment, judgment is made based on the speed difference set. When there is no speed difference item that reaches the change threshold in the speed difference set, the detection interval item is extended based on the positive growth ratio. When there is a speed difference item that reaches the change threshold in the speed difference set, the detection interval item is shortened based on the negative growth ratio.

3. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 2, characterized in that: The method for obtaining the temperature change item includes: N1: feature point acquisition, based on the speed difference item, positive and negative judgment is performed. When the speed difference item is negative, the two real-time speed items constituting the speed difference item are respectively set as the first speed feature point and the second speed feature point; N2: The temperature change item is determined, and the target temperature corresponding to the first speed characteristic point is used as the temperature change item.

4. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 3, characterized in that: The method for obtaining the temperature change item also includes: setting an extended threshold value, setting the extended threshold value, in step N1, when the speed difference item is a negative number, obtaining the speed difference item within the target extended threshold value again based on the extended threshold value, and performing a positive and negative judgment again, when the speed difference items within the target extended threshold value are all negative numbers, selecting the first negative speed difference item as the target difference item, and repeating steps N1-N2, when there is a positive number in the speed difference item within the target extended threshold value, setting the first negative speed difference item as the comparison difference item, repeating steps N1-N2, using the obtained temperature change item as the comparison temperature item, repeating steps N1-N2 again, and then obtaining the extended temperature item, and comparing it with the comparison temperature item, when the extended temperature item is greater than the comparison temperature item, using the extended temperature item as the temperature change item, otherwise using the comparison temperature item as the temperature change item.

5. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 1, characterized in that: The stage division method includes: M1: Change point setting, set the detection time of the labeled isotope, take the detection time as the change point, and get the first range and the second range. The first range is from the start of the reaction to the change point, and the second range is from the change point to the end of the reaction; M2: stage correspondence, based on the first range and the second range corresponding to the first stage and the second stage respectively, the first range corresponds to the first stage, and the second range corresponds to the second stage.

6. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 5, characterized in that: The method for stage confirmation includes: stage judgment, confirming whether the stage of the reaction has reached a change point, and then performing stage confirmation. When the stage of the reaction has not reached the change point, confirming that the stage of the reaction is the first stage, when the stage of the reaction has reached the change point, confirming that the stage of the reaction is the second stage.

7. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 1, characterized in that: The method for obtaining the threshold item of the first stage includes: X1: Image acquisition, obtaining the state images of labeled isotopes at different temperatures to obtain a state image set; X2: critical point determination, based on the state image set, determine the highest critical temperature of the target marker isotope and obtain the critical temperature term; X3: Protection threshold setting. Set the protection threshold. Based on the protection threshold and the critical speed corresponding to the critical temperature item, set the decrease range of the critical speed item to obtain the first stage threshold item.

8. The method for tracing the source of carbon and nitrogen elements based on isotope labeling according to claim 7, characterized in that: The method for obtaining the second-stage threshold item includes: determining the lowest critical temperature of the target marker isotope based on the state image set, and setting the speed increase amplitude based on the protection threshold and the critical speed corresponding to the lowest critical temperature to obtain the second-stage threshold item.

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