Fluid device

Abstract

The present disclosure provides a fluid device comprising: a device main body having a disc-shaped space therein for accommodating a fluid; a fluid inlet configured so as to introduce a fluid in a tangential direction at a position of 0 o'clock in a substantially circumferential portion of the disc-shaped space; and a fluid outlet configured so as to lead the fluid out of the space at a position of 6 o'clock to 12 o'clock in a substantially circumferential portion of the disc-shaped space.

Description

Technical Field

[0001] The present disclosure relates to fluidic devices. Background Art

[0002] In order to perform measurements on small amounts of liquid samples, microfluidic devices with small volumes have been developed.

[0003] When the fluid is introduced into the flow channel device, gaps or dead zones are easily generated in the corners of the space. In addition, due to the resistance of the wall, the target solution is sometimes introduced into the more central part of the volume, and the previous liquid remains near the wall. Therefore, an unbalanced solution distribution is generated in the fluid device. As long as the volume of the fluid device is large, the proportion of these problems in the measurement results is small, and the measurement can be performed in the part where no problems occur. In other words, these problems can be ignored. Summary of the invention

[0004] However, as the flow channel device becomes smaller, the generation or residue of air gaps or the uneven distribution of the target solution relative to the measurement area or volume becomes larger, and the behavior of the fluid in the microspace becomes significant. Therefore, these problems may have a substantial impact on the measurement results or measurement efficiency of the microfluid. However, such a problem is only an example, and the subject of the present disclosure described below is not limited to this.

[0005] Therefore, in microfluidic devices, it is desirable to improve the generation or retention of voids and the exchange efficiency of fluids. The present disclosure describes and provides a microfluidic device that suppresses or avoids these or other problems.

[0006] In one embodiment of the present disclosure, a fluid device is provided, comprising: a device body, which has a disc-shaped space for containing a fluid inside; a fluid inlet, which is configured to introduce the fluid in a clockwise and tangential direction at the 0 o'clock position on the substantial circumference of the above-mentioned disc-shaped space; and a fluid outlet, which is configured to discharge the fluid from the above-mentioned space at the 6 o'clock to 12 o'clock position on the substantial circumference of the above-mentioned disc-shaped space.

[0007] According to some embodiments of the present disclosure, for example, it is possible to efficiently perform high-precision measurement on a relatively small amount of sample.

[0008] Other modes and advantages of the present disclosure will be readily apparent to those skilled in the art from the following detailed description which merely illustrates and describes the exemplary embodiments of the present disclosure. As will be appreciated, the present disclosure may be in other different embodiments, some of the details of which may be modified in various obvious ways without departing from the present disclosure. Therefore, the drawings and descriptions are to be regarded as illustrative in nature and not as limiting. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 It is a top view schematically showing a fluid device according to a comparative example.

[0010] Figure 2 It is a top view schematically showing a fluid device according to a comparative example.

[0011] Figure 3 It is a top view schematically showing fluid devices according to some embodiments.

[0012] Figure 4 It is a top view schematically showing fluid devices according to some embodiments.

[0013] Figure 5 1 and 2 are a plan view (A) and a perspective view (B) schematically showing a fluid device according to an embodiment.

[0014] Figure 6 1 and 2 are a plan view (A) and a perspective view (B) schematically showing a fluid device according to an embodiment.

[0015] Figure 7 It is a top view schematically showing a fluid device according to one embodiment.

[0016] Figure 8 The present invention is an exploded perspective view of a fluid device according to an embodiment.

[0017] Fig. 9 It is a top view schematically showing a fluid device according to a comparative example.

[0018] Fig.10 It is a graph showing the output relative to the introduction amount in the fluid device according to one embodiment and the fluid device according to a comparative example.

[0019] Fig.11 This is a diagram showing the temporal change of the output at each height of the disk-shaped space according to one embodiment.

[0020] Fig.12 This is a diagram showing the relationship between the height of the disk-shaped space and the holding time of the maximum output according to one embodiment. DETAILED DESCRIPTION

[0021] The "disc-shaped space" used in this specification generally refers to a cylindrical space. The size of the cross section of the cylinder (the size of the major diameter and / or minor diameter, the diameter or the radius) is equal to or greater than the size in the direction of the central axis. In several embodiments, the cylinder of the "disc-shaped space" has a substantially circular cross section, and its diameter or radius is greater than the size in the direction of the central axis (the height of the disc-shaped space).

[0022] The "inlet of the fluid" (referred to as "fluid inlet", "inlet") and / or the "outlet of the fluid" (referred to as "fluid outlet", "outlet") have a tubular space (flow channel) inside, and may be detachably connected to the device body, may be fixed to the device body, or may be formed or manufactured integrally with the device body. The inlet and / or outlet may refer to the flow channel itself.

[0023] 0 o'clock used in this specification refers to the position of the angular direction of the disc space where the fluid flowing through the fluid inlet actually enters the disc space. It is also possible to define 0 o'clock as the direction from the center of the disc space toward the connection position of the fluid inlet and the disc space. 0 o'clock can be defined as the radial direction perpendicular to the circumferential direction when the fluid is introduced. The introduced fluid or at least a part thereof flows along the circumference near the circumference of the disc-shaped space. In this specification, the direction of the flow is defined as the clockwise direction.

[0024] The inlet and / or outlet are arranged on the circumference, substantially on the circumference or near the circumference. The "circumference" used in this specification refers to the circumference of the disc of the disc-shaped space of the fluid device or its vicinity. This does not refer to a position on the geometric circumference, but refers to a position or position that is at least separated from the geometric circumference by the distance required to introduce the fluid into the disc-shaped space or to lead it out from the disc-shaped space. For example, the inlet and / or outlet has a tube structure, and at least a part or all of the diameter of the tube structure is contained inside the disc. In this case, the center of the inlet or outlet tube does not exist on the circumference of the disc. Alternatively, due to manufacturing reasons, the inlet and / or outlet sometimes cannot be formed on the circumference or its outer edge is formed tightly on the circumference.

[0025] "Disc-shaped space" refers to a space formed in a cylindrical shape. In some embodiments, the cross section perpendicular to the central axis of the cylinder can be a perfect circle. In some embodiments, the cross section of the cylinder can be a non-perfect circle, for example, it can be an ellipse. The circumferential surface of the disc-shaped space can be substantially formed by a curved surface, preferably a continuous curved surface.

[0026] In some embodiments, the fluid inlet can be configured to introduce fluid in a direction perpendicular to the central axis of the cylinder of the disk-shaped space, that is, in the plane of the disk. In some embodiments, the disk-shaped space can be connected to the side of the disk from the outside.

[0027] In several embodiments, the fluid inlet can be configured to introduce fluid in a direction perpendicular to the central axis of the cylinder of the disc-shaped space, i.e., in a direction inclined from the in-plane direction of the disc. In several embodiments, the fluid inlet can be connected to the bottom surface of the cylinder. In several embodiments, the fluid inlet can be connected in a direction that is not perpendicular to the bottom surface of the cylinder or in a direction inclined to the bottom surface of the cylinder. That is, the direction of the fluid inlet projected onto the bottom surface of the cylinder is toward the circumferential direction of the cylinder.

[0028] In several embodiments, the inclined inlet and outlet can be connected to the same bottom surface of the disc-shaped space. In several embodiments, the inclined inlet can be connected to the first bottom surface (one bottom surface), and the outlet can be connected to the second bottom surface (another bottom surface).

[0029] In several embodiments, the two bottom surfaces may not necessarily be parallel. For example, the bottom surface may not be a plane. For example, at least a portion may be formed into a cone (convex or concave relative to the disc space). For example, one bottom surface of the cylinder may be formed in a manner away from the other bottom surface near the fluid inlet (convex cone). Thus, for example, bubbles in the disc space are easily discharged from the fluid outlet. For example, one bottom surface of the cylinder may be formed in a manner close to the other bottom surface near the fluid inlet (concave cone). The fluid introduced into the disc space easily passes near the circumference. The water flow on the circumferential side with a relatively small solution exchange rate can be enhanced to improve the solution exchange rate.

[0030] The height of the disk-shaped space can be 10μm, 15μm, 20μm, 25μm, 30μm, 40μm, 50μm, 60μm, 70μm, 80μm, 90μm, 100μm, 150μm, 200μm, 300μm, 400μm, 500μm, 600μm, 700μm, 800μm, 900μm, 1000μm, etc. or a value larger than it.

[0031] The height of the disk-shaped space can be 10μm, 15μm, 20μm, 25μm, 30μm, 40μm, 50μm, 60μm, 70μm, 80μm, 90μm, 100μm, 150μm, 200μm, 300μm, 400μm, 500μm, 600μm, 700μm, 800μm, 900μm, 1000μm, 1.5mm, 2mm, etc. or a value smaller than these.

[0032] The characteristic quantity of the radius or surface direction of the disk-shaped space can be 1mm, 2mm, 3mm, 4mm, 5mm, 6mm, 7mm, 8mm, 9mm, 10mm, etc. or a value larger than these.

[0033] The characteristic quantity of the radius or surface direction of the disk-shaped space can be 5mm, 6mm, 7mm, 8mm, 9mm, 10mm, 11mm, 12mm, 13mm, 14mm, 15mm, 16mm, 17mm, 18mm, 19mm, 20mm, etc. or a value smaller than these.

[0034] The volume of the disc-shaped space can be 1μL, 2μL, 3μL, 4μL, 5μL, 6μL, 7μL, 8μL, 9μL, 10μL, 15μL, 20μL, 30μL, 40μL, 50μL, 60μL, 70μL, 80μL, 90μL, 100μL, 200μL, 300μL, 400μL, 500μL, etc. or a value larger than it.

[0035] The volume of the disc-shaped space can be 5μL, 6μL, 7μL, 8μL, 9μL, 10μL, 15μL, 20μL, 30μL, 40μL, 50μL, 60μL, 70μL, 80μL, 90μL, 100μL, 200μL, 300μL, 400μL, 500μL, 1mL, etc. or a smaller value.

[0036] The fluid inlet may substantially have a volume of 50% to 200% of the volume of the disc-shaped space. The volume of the fluid inlet may substantially be 50%, 100%, 150% or 200% of the volume of the disc-shaped space. The sum of the volume of the disc-shaped space and the volume of the inlet (the volume from the fluid introduction portion to the inlet to the disc-shaped space) may be 2 μL, 3 μL, 4 μL, 5 μL, 6 μL, 7 μL, 8 μL, 9 μL, 10 μL, etc., or a value greater than that. The sum of the volume of the disc-shaped space and the volume of the inlet may be 10 μL, 15 μL, 20 μL, 30 μL, 40 μL, 50 μL, etc., or a value less than that.

[0037] In some embodiments, the fluidic device may have a sensor on the inner wall of the disc-shaped space. In some embodiments, the sensor may be disposed on one or both bottom inner walls of the disc-shaped space.

[0038] <Measurement object>

[0039] In some embodiments, the subject (subject) may include or be a human. In some embodiments, the subject may include or be an animal other than a human. The subject may include or be a mammal. The subject may be, for example, a working animal, a livestock animal, a pet animal, or a wild animal, but is not limited thereto.

[0040] The sample to be measured may be a solution. The "solution" may be a body fluid, a solution derived from a body fluid, or a dilution of a body fluid. The solution may be a non-body fluid (non-body fluid source) solution, or a body fluid or a mixture of a solution derived from a body fluid and a solution non-body fluid source. The solution may be a solution used in sample measurement, or a solution used in calibration measurement. For example, a solution may be a standard solution or a calibration solution. For example, a solution may be a liquid that intentionally or intentionally does not contain a substance to be measured in order to be used for calibration, etc. The sample to be measured may be a specimen. The solution may be a solution containing chemical substances.

[0041] "Body fluid" can be lymph fluid, can be tissue fluid such as interstitial fluid, intercellular fluid, interstitial fluid, can also be body cavity fluid, serous cavity fluid, pleural effusion, ascites, pericardial fluid, cerebrospinal fluid (cerebrospinal fluid), joint fluid (synovial fluid), aqueous humor (aqueous humor). Body fluid can be digestive fluid such as saliva, gastric juice, bile, pancreatic juice, intestinal juice, can also be sweat, tears, nasal mucus, urine, semen, vaginal fluid, amniotic fluid, breast milk. Body fluid can be animal body fluid or human body fluid. "Body fluid" can be a solution. The solution can also contain physiological buffers such as phosphate buffered saline (PBS) and N-tris (hydroxymethyl) methyl-2-aminoethane sulfonic acid buffer (TES) containing the substance to be measured. There is no particular limitation as long as the solution contains the substance to be measured.

[0042] The solution may contain the substance to be measured. The solution may have the possibility of containing the substance to be measured. In several embodiments, the substance to be measured may be a molecule, an ion, a polymer, a biomolecule, etc. The substance to be measured may also have a biomolecule. The substance to be measured may be a protein, a glycated protein, etc. For example, the solution is tears, and the substance to be measured may be albumin, glycated albumin, hemoglobin, or glycated hemoglobin contained in tears. Alternatively, the substance to be measured may be albumin, glycated albumin, hemoglobin, or glycated hemoglobin in blood, serum, or plasma, or may be albumin, glycated albumin, hemoglobin, or glycated hemoglobin in interstitial fluid, urine, or saliva. Albumin may be oxidized albumin (HNA) or reduced albumin (HMA). In several embodiments, the substance to be measured may be AGE (Advanced Glycation End Products). In several embodiments, the substance to be measured may be glycated lipid.

[0043] <Sensor>

[0044] The sensor may be a chemical sensor, a biosensor, an ion sensor, or the like (hereinafter sometimes referred to as a "sensor," "biochemical sensor," "chemical sensor," or "electrochemical sensor.") The sensor may include a plurality of sensors.

[0045] The sensor may have an electrode. The electrode may be an amperometric electrode. The electrode may be a hydrogen peroxide electrode. The electrode may be an oxygen electrode. The electrode may be a potentiometric electrode. The electrode may be an ion detection electrode (pH electrode, cyanide ion electrode, iodide ion electrode, etc.).

[0046] In several embodiments, the sensor can output an electrical signal. In several embodiments, the sensor can output a current signal. The sensor can output a voltage signal or a charge. The sensor can be electrically connected to an ammeter, a voltmeter, etc.

[0047] In several embodiments, the sensor can have an enzyme film on an electrode.

[0048] In several embodiments, the enzyme membrane may include a protease. "Protease" is generally a general term for peptide bond hydrolases that hydrolyze proteins and polypeptides and perform decomposition metabolism. Protease can be an enzyme that breaks down proteins into peptide fragments. In the case where the protein contains glycosylated amino acid residues, there may be peptide fragments containing glycosylated amino acid residues and completely unglycosylated peptide fragments in the peptide fragments produced by the action of the protease.

[0049] The "protease" may be a protease from an animal, a protease from a plant, or a protease from a microorganism. The protease may be an exopeptidase or an endopeptidase. The protease may be an aspartic protease, a metalloprotease, a serine protease, or a thiol protease.

[0050] "Protease" may include multiple types or kinds of proteases, or may include one type or kind of protease. For example, the protease may include one of a protease and a peptidase, or may include both. By mixing multiple proteases, the decomposition efficiency can sometimes be improved. The protease may include a modified protease or a modified protease. The protease may be used together with an additive. The additive may be, for example, a surfactant or urea. The additive may, for example, make the protein unstable or denatured, but is not limited to this. By using a modified protease and an additive, for example, the decomposition efficiency of the protein and the selectivity of the substrate may be improved, but is not limited to this.

[0051] A "substrate" is a substance that reacts chemically by an enzyme. Alternatively, a substrate is a substance that binds to an enzyme protein, which, by binding to the enzyme, reduces the activation energy of a specific chemical reaction, resulting in conversion to a specific product at an amazing rate.

[0052] In several embodiments, the enzyme membrane may include an oxidase. An "oxidase" is an enzyme that uses a molecular oxygen substrate as an electron acceptor. Alternatively, an oxidase is an enzyme that catalyzes a redox reaction using molecular oxygen as an acceptor of hydrogen or electrons.

[0053] In several embodiments, the oxidase may comprise ketoamine oxidase. In several embodiments, the oxidase may comprise glucose oxidase. Using the glucose oxidase, the glucose concentration in the sample can be determined. In this case, the substrate can comprise glucose. In several embodiments, the oxidase may comprise alcohol oxidase. In this case, the substrate can comprise primary alcohol. Using it, the alcohol concentration in the sample can be determined.

[0054] "Ketoamine oxidase" generally refers to an oxidase that recognizes the ketoamine structure of a glycated amino acid or a peptide or peptide fragment containing a glycated amino acid residue and oxidizes the glycated amino acid to produce amino acids, glucosone (α-ketoaldehyde) and hydrogen peroxide. Therefore, ketoamine oxidase generates hydrogen peroxide in a concentration proportional to or related to the concentration of the recognized glycated amino acid or a peptide or peptide fragment containing a glycated amino acid residue.

[0055] Ketoamine oxidase may be a dehydrogenase, a kinase, or an oxidase. Ketoamine oxidase may be fructosyl amino acid oxidase (FAOD), fructosyl peptide oxidase, fructosyl valyl histidine oxidase, fructosamine oxidase, amadoriase, fructosamine desaccharidase, or modified forms thereof.

[0056] In some embodiments, the sensor can measure glycated proteins. In some embodiments, a protease and a ketoamine oxidase can be arranged on or near the surface of the electrode. In some embodiments, the enzyme membrane can have a protease and a ketoamine oxidase. The method for measuring glycated proteins using a general enzymatic method is as follows: first, in the first step, the protein is decomposed into amino acids using a protease, in the second step, ketoamine oxidase is allowed to act only on the glycated amino acids among these amino acids to generate hydrogen peroxide, and in the third step, the hydrogen peroxide is optically or electrically measured.

[0057] In several embodiments, the sensor may have a detection unit. The detection unit may be a hydrogen peroxide detection unit. The "hydrogen peroxide detection unit" (hydrogen peroxide sensor) may be an electrochemical electrode or a hydrogen peroxide electrode. The hydrogen peroxide electrode may have a counter electrode, a reference electrode, and a working electrode. In a certain embodiment, the detection unit may detect oxygen. For example, the amount or concentration of oxygen reduced in an enzyme reaction may be detected. It is believed that oxygen detection has low sensitivity to molecules and ions that become noise sources and has strong anti-interference. The oxygen consumption may also be measured by oxygen detection. Since the detection unit becomes saturated with the atmosphere, it may also be used for enzyme sensing detection. The detection unit may also be constructed in a manner that allows for selective or combined use of multiple detection methods.

[0058] <Optical measurement>

[0059] In some embodiments, the fluidic device body may be constructed in a manner that allows light to be introduced from the outside into the interior of the disk-shaped space. The fluidic device body may be at least partially transparent.

[0060] The fluidic device may include an optical device or may be configured to be connected to an optical device.

[0061] As used in this specification, "optical measurement" generally refers to the use of optical elements or devices to determine the optical properties of a substance. In several embodiments, an optical measurement of a target substance can be performed. In several ways, the properties of a substance that is bound to or associated with a target substance (hereinafter, not the target substance itself, but a substance that is chemically, biologically or physically bound to or associated with the target substance (e.g., a reagent)) can be measured. The properties of a reagent can be measured. Sometimes the reagent is referred to as the "target substance."

[0062] In some embodiments, the optical measurement can include spectroscopic measurement. For example, the absorbance of the target substance can be measured. In some embodiments, a colorimetric indicator corresponding to the target substance can be introduced. The colorimetric indicator can be detected or measured.

[0063] The fluid device may have a plurality of disc-shaped spaces. Each disc may have an inlet and an outlet. Fluid may be provided to the plurality of inlets and disc-shaped spaces from a common flow channel. Fluid may be provided to each of the plurality of inlets and disc-shaped spaces individually. The plurality of disc-shaped spaces that the fluid device has may have substantially the same volume, or may have different volumes between them.

[0064] In some embodiments, the fluidic device may be a stopped-flow fluidic device. After the introduced fluid fills the disc-shaped space, the introduction of the fluid may be stopped. Thereafter, a predetermined measurement or sensing may be performed on the fluid in the disc-shaped space.

[0065] In some embodiments, a predetermined measurement or sensing detection can be performed on the fluid in the disc-shaped space while the introduced fluid flows through the disc-shaped space.

[0066] <Comparative Example 1>

[0067] exist Figure 1 A is a schematic diagram of a fluid device 100 as a comparative example. The fluid device 100 has a disc-shaped space 120 formed in a body 110. A fluid inlet 130 and a fluid outlet 140 are fluidically connected to the disc-shaped space 120. Figure 1 The fluid inlet 130 of A is arranged in the cross-sectional direction. The fluid 151 (solid arrow) passing through the fluid inlet 130 is introduced into the disc-shaped space 120 perpendicularly to the 0 o'clock direction of the disc-shaped space 120, that is, along the circumferential direction. Figure 1The fluid outlet 140 of A is arranged substantially at the center of the disc-shaped space 120 , and guides the internal fluid to the outside of the disc-shaped space 120 from the bottom surface (not shown).

[0068] Figure 1 A shows a cross section perpendicular to the central axis of the disk-shaped space 120. The fluid outlet 140 is actually connected to any bottom surface of the disk-shaped space 120 and is not present in the cross section, but is shown in the same figure for the sake of explanation. The same applies to the following figures.

[0069] Figure 1 B shows the Figure 1 A is a fluid device 100 of the same type as in FIG. 1, and an example of the flow of a fluid 151 in a disc-shaped space 120 is described using this figure. The fluid 151 enters the disc-shaped space 120 through the fluid inlet 130, and then flows in a manner passing through its circumference (refer to the dotted line 152). However, the fluid outlet 140 is located in the center of the disc-shaped space 120. Therefore, the fluid 152 flowing through the circumference does not go around the circumference, but leaves the circumference, for example, from around 6 o'clock to 9 o'clock. Then, the fluid 152 flows toward the fluid outlet 140 located in the center of the disc-shaped space 120 or is pulled toward the fluid outlet 140. Therefore, a liquid surface 153 is formed, and a space (sometimes called a bubble) 154 in which no liquid exists is formed as a boundary. As a result, the disc-shaped space 120, especially all parts of its bottom surface, cannot be filled with fluid.

[0070] <Comparative Example 2>

[0071] exist Figure 2 A schematic diagram of a fluid device 200 as a comparative example is shown. The fluid device 200 has a disc-shaped space 220 formed in a body 210. A fluid inlet 230 and a fluid outlet 240 are fluidically connected to the disc-shaped space 220. Figure 2 The fluid inlet 230 of A is arranged in the cross-sectional direction. The fluid 251 (solid arrow) passing through the fluid inlet 230 is introduced into the disc-shaped space 220 perpendicularly to the 0 o'clock direction of the disc-shaped space 220, that is, along the circumferential direction. Figure 2 The fluid outlet 240 of A is arranged near the circumference of the disc-shaped space 220 at the 3 o'clock position, and discharges the internal fluid to the outside of the disc-shaped space 220 from the bottom surface (not shown).

[0072] Figure 2 B shows the Figure 2A is a fluid device 200 of the same type as in FIG. 2, and an example of the flow of a fluid 251 in a disc-shaped space 220 is described using this figure. The fluid 251 enters the disc-shaped space 220 through the fluid inlet 230, and then flows in a manner passing through its circumference (refer to the dotted line 252). The fluid outlet 240 is located at the 3 o'clock position of the disc-shaped space 220. A portion of the fluid 252 flowing through the circumference is discharged to the outside at the fluid outlet 240, and the rest flows further along the circumference. However, the fluid 252 does not go around the circumference, but leaves the circumference, for example, from around 6 o'clock to 9 o'clock. Then, the fluid 252 flows in a manner toward the fluid outlet 240 or is pulled toward the fluid outlet 240. Therefore, a liquid surface 253 is formed, and a space (sometimes called a bubble) 254 in which no liquid is present is formed as a boundary. As a result, the disc-shaped space 220, especially all parts of its bottom surface, cannot be filled with fluid.

[0073] exist Figure 1 and Figure 2 The fluid 152, 252 (flow shown by the dotted line) inside the disc-shaped space 120, 220 shown in the figure is exemplary and should not be interpreted as being limited thereto. Other fluid flows may also exist. In several embodiments, the introduced fluid can fill the disc-shaped space 120, 220.

[0074] <Forms of Fluid Devices>

[0075] exist Figure 3 A to 3D show the configurations of fluid devices according to some embodiments. Figure 3 A shows the fluid device 300 in which the fluid outlet 340 is arranged at the 6 o'clock position near the circumference of the disc-shaped space 320 . Figure 3 B shows the fluid device 400 in which the fluid outlet 440 is arranged at a position of about 7:30 near the circumference of the disc-shaped space 420 . Figure 3 C shows the fluid device 500 in which the fluid outlet 540 is arranged at the 9 o'clock position near the circumference of the disc-shaped space 520. Generally, when the fluid outlet is located closer to the fluid inlet, bubbles are less likely to remain in the disc-shaped space.

[0076] Figure 3 D shows the fluid device 600 in which the fluid outlet 640 is arranged at a position of about 10 o'clock near the circumference of the disc-shaped space 620 . Figure 3 The outlet 640 of D is arranged as close to the fluid inlet 630 as possible without interfering with the fluid inlet 630. At this position, the fluid introduced from the fluid inlet 630 meets the fluid flowing along the circumference of the disc-shaped space 620, and turbulence is likely to occur. Therefore, air (space without liquid or bubbles) is likely to remain. The fluid outlet 640 can Figure 1 B and Figure 2The bubbles visible in B are effectively vented to the outside.

[0077] like Figure 1 to Figure 3 As shown, the cross section of the disc-shaped space may be a circle. Alternatively, the cross section of the disc-shaped space may not be a circle, but may be other curves, or may be composed of multiple curved surfaces. In several embodiments, the cross section of the disc-shaped space may be an ellipse.

[0078] exist Figure 4 A and Figure 4 B shows a fluidic device 700, 800 having an elliptical disk-shaped space. Figure 4 The fluid device 700 shown in A has an elliptical major axis in the 0 o'clock-6 o'clock direction of the disc-shaped space 720. The fluid 751 is introduced into the disc-shaped space 720 along the circumferential direction at the 0 o'clock position as one end of the major axis through the fluid inlet 730. Figure 4 The fluid device 800 shown in B has an elliptical major axis in the 3 o'clock to 9 o'clock direction of the disc-shaped space 820. The fluid 851 is introduced into the disc-shaped space 820 along the circumferential direction at the 0 o'clock position as one end of the minor axis using the fluid inlet 830. In any case, the fluid outlets 740, 840 are arranged at about 10 o'clock or at a position close to the fluid inlets 730, 830 but not interfering with the fluid inlets 730, 830.

[0079] In some embodiments, the fluid inlet may be arranged in the in-plane direction of the cross section of the disc-shaped space. In some embodiments, the fluid inlet may be arranged at an angle relative to the cross section of the disc-shaped space.

[0080] exist Figure 5 2 shows a fluid device 900 according to an embodiment in which a fluid inlet is arranged in the in-plane direction of a cross section of a disk-shaped space. Figure 5 A shows a cross-section or top view parallel to the bottom surface of the fluidic device 900 . Figure 5 B is a perspective view of the fluid device 900 , in which the main body 910 is omitted. The fluid device 900 has a fluid inlet 930 arranged in a plane parallel to the bottom surface of the disk-shaped space 920 .

[0081] The fluid inlet 930 introduces the fluid 951 along the in-plane direction of the disc-shaped space 920 at the 0 o'clock position (actually, from the position near 11 o'clock to the 0 o'clock (12 o'clock) position). The fluid (dashed line 952) flows mainly along the circumference in the disc-shaped space 920 and fills the disc-shaped space 920. The fluid 952 is finally discharged from the fluid outlet 940 arranged in a direction substantially perpendicular to a bottom surface of the disc-shaped space 920.

[0082] exist Figure 62 shows a fluid device 1000 according to an embodiment in which a fluid inlet is arranged at an angle with respect to a cross section of a disk-shaped space. Figure 6 A shows a cross-section or top view parallel to the bottom surface of the fluidic device 1000 . Figure 6 B is a perspective view of the fluid device 1000 , in which the main body 1010 is omitted. The fluid device 1000 has a fluid inlet 1030 disposed in a direction of an angle θ with respect to the bottom surface of the disk-shaped space 1020 .

[0083] The fluid inlet 1030 introduces the fluid 1051 into the disc-shaped space 1020 from the direction of the angle θ at the position of 0 o'clock of the disc-shaped space 1020 (actually, from the position of about 11 o'clock to 0 o'clock (12 o'clock)). The fluid (dashed line 1052) flows mainly along the circumference in the disc-shaped space 1020 and fills the disc-shaped space 1020. The fluid 1052 is finally discharged from the fluid outlet 1040 arranged in a direction substantially perpendicular to a bottom surface of the disc-shaped space 920.

[0084] <Fluid device with sensor-1>

[0085] In some embodiments, the fluidic device may have a sensor inside the disc-shaped space thereof. The sensor is capable of sensing and detecting the fluid introduced into the disc-shaped space or a substance contained in the fluid.

[0086] exist Figure 7 1 shows a top view of a fluid device 1100 according to one embodiment. However, the solid lines and the dotted lines should not be interpreted as corresponding to whether they are visible from the top surface, but are merely schematically showing the structure of the internal components of the fluid device 1100.

[0087] The fluid device 1100 has a disc-shaped space 1120 inside a main body 1110. A fluid inlet 1130 and a fluid outlet 1140 are formed for the disc-shaped space 1120. The fluid inlet 1130 is arranged in a manner inclined relative to the bottom surface of the disc-shaped space 1120, and the fluid outlet 1140 is arranged in a direction perpendicular to the bottom surface of the disc-shaped space 1120. The fluid introduction port 1131 is formed at a right angle to the plane of the main body 1110 or the disc-shaped space 1120, and the fluid introduced from the outside is introduced into the fluid introduction port 1131. Thereafter, the fluid enters the fluid inlet 1130 and is introduced into the inside of the disc-shaped space 1120 from the inclined direction. The fluid outlet 1140 is formed at a right angle to the plane of the main body 1110 or the disc-shaped space 1120. The fluid is discharged from the disc-shaped space 1120 to the outside through the fluid outlet 1140.

[0088] Sensing electrodes 1121, 1122, and 1123 are arranged in the disc-shaped space 1120. In the present embodiment, the sensing electrode is an electrode for electrochemical measurement having three electrodes. The sensing electrode has a working electrode 1121, a counter electrode 1122, and a reference electrode 1123. These electrodes are connected to corresponding connection terminals 1161, 1162, and 1163 via lead wires. The connection terminals are exposed on the outer surface of the main body 1110 and can be electrically connected from the outside.

[0089] <Fluid device with sensor-2>

[0090] exist Figure 8 2 shows an exploded perspective view of a fluid device 2000 according to an embodiment. The fluid device 2000 includes, from top to bottom, a pipette inlet 2100, an upper surface seal 2200, a flow channel unit 2300, an adhesive film 2400, a sensor chip 2500, a sensor chip support 2600, an O-ring 2700, and a waste liquid tank 2800, which are combined in the vertical direction.

[0091] The sensor chip support 2600 has a sensor chip receiving portion 2680 in which the sensor chip 2500 is disposed. The flow channel unit 2300 is closely attached thereto via the adhesive film 2400 .

[0092] The flow channel unit 2300 has a disk-shaped depression 2320 on its lower surface. The disk-shaped depression 2320, the surface of the electrode portion 2520 on the upper surface of the sensor chip 2500 facing thereto, and the through hole 2320 of the adhesive film 2400 interposed therebetween define a disk-shaped space.

[0093] The upper surface seal 2200 is bonded and sealed to the upper surface of the flow channel unit 2300. A pipette inlet 2100 is arranged thereon.

[0094] The pipette inlet 2100 has a fluid inlet channel 2130 and a pipette receiving portion 2131 at the center, the pipette receiving portion 2131 receiving the front end of the pipette and continuing to the fluid inlet channel 2130. The upper surface seal 2200 has a channel inlet hole 2230 leading from the pipette inlet 2100 to the channel unit 2300.

[0095] The sensor chip support 2600 is pressed against the waste liquid tank 2800 via the O-ring 2700. The flow channel unit 2300 located above it has a fitting claw 2380, and the fitting claw 2380 fits with the claw receiving portion 2880 of the waste liquid tank 2800. Thus, the pipette inlet 2100, the upper surface seal 2200, the flow channel unit 2300, the adhesive film 2400, the sensor chip 2500, and the sensor chip support 2600 assembled by bonding are closely fitted to the waste liquid tank 2800.

[0096] The fluid inlet channel 2130 of the pipette inlet 2100, the channel inlet hole 2230 of the upper surface seal 2200, and the upper surface opening of the fluid inlet 2330 of the channel unit 2300 are aligned. Thus, the fluid introduced from the pipette (not shown) is introduced into the disc-shaped space 2320 through the fluid inlet channel 2130 of the pipette inlet 2100, the channel inlet hole 2230 of the upper surface seal 2200, and the fluid inlet 2330 of the channel unit 2300.

[0097] The contact opening 2260 of the upper surface seal 2200, the contact through hole 2360 of the flow channel unit 2300, and the contact opening 2460 of the adhesive film 2400 are aligned in a manner arranged on the upper portion of the contact terminal 2560 of the sensor chip 2500. Thus, a connection pin (not shown) can approach the contact terminal 2560 of the sensor chip 2500 through these openings and through holes from the outside of the fluid device 2000 and make an electrical connection. In this way, the surface electrochemical measurement of the introduced liquid can be performed via the electrode portion 2520 of the sensor chip 2500, and an electrical signal can be obtained.

[0098] The fluid (waste liquid) flowing out of the disc-shaped space 2320 of the flow channel unit 2300 flows upward from the fluid outlet 2340 of the flow channel unit 2300, passes through the channel 2341 formed by the groove 2341 formed on the upper surface of the flow channel unit 2300 and the upper surface seal 2200, and is guided downward from the second fluid outlet 2342. Thereafter, the waste liquid passes through the through hole 2440 of the adhesive film and the through hole 2640 of the sensor chip support 2600 and falls into the waste liquid receiving space 2840 of the waste liquid tank 2800, where it is recovered in a sealed manner.

[0099] The air opening 2270 of the upper surface seal 2200, the air through hole 2370 of the flow channel unit 2300, the air opening 2470 of the adhesive film 2400, and the air through hole 2670 of the sensor chip support 2600 are aligned. That is, the waste liquid receiving space 2840 of the waste liquid tank 2800 is connected to the external fluid of the fluid device 2000 to form an air vent. Thus, as the waste liquid is introduced into the waste liquid receiving space 2840, the air present in the waste liquid receiving space 2840 sealed by the O-ring 2700 can escape to the outside. In other words, the waste liquid can flow into the receiving space 2840 in the waste liquid tank 2800.

[0100] <Comparative example: Straight flow channel>

[0101] exist Fig. 92 shows a top view of a fluid device 1200 having a linear flow channel as a comparative example. However, the solid lines and dashed lines should not be interpreted as corresponding to whether they are visible from the top surface, but are merely schematically showing the structure of the internal components of the fluid device 1200.

[0102] The fluid device 1200 has a linear (straight-shaped) space 1220 inside a body 1210. A fluid inlet 1230 and a fluid outlet 1240 are formed near the end of the linear space 1220. The fluid inlet 1230 and the fluid outlet 1240 are formed perpendicularly to the plane of the body 1210 or the linear space 1220. The fluid is introduced into the linear space 1220 from the fluid inlet 1230, flows in the linear space 1220 along its length direction, fills the linear space 1220, and is discharged to the outside from the fluid outlet 1240.

[0103] In the linear space 1220, sensing electrodes 1221, 1222, and 1223 extending in the length direction thereof are arranged. In this comparative example, the sensing electrode is an electrode for electrochemical determination having three electrodes. The sensing electrode has a working electrode 1221, a reference electrode 1222, and a counter electrode 1223. These electrodes are connected to corresponding connection terminals 1261, 1262, and 1263 via lead wires. The connection terminals are exposed on the outer surface of the main body 1210, and electrical connection can be formed from the outside.

[0104] <Comparison between disc type and linear type>

[0105] It is generally believed that in a sensing device using a stopped-flow method, the sensing performance is reduced when the measuring liquid is of low volume. It can be speculated that one of the reasons is that in the stopped-flow method, it is difficult to completely replace the measuring space filled with the front liquid with the measuring liquid, and it is difficult to remove the mixed bubbles. Therefore, in this experimental example, the amount of liquid introduced into the disc-shaped flow channel and the linear flow channel, especially the sensing performance under low volume, was compared. The structure of the device used is as follows.

[0106] The disc-shaped flow channel has Figure 7 The structure shown is the same as the structure shown. The shape of the disc-shaped space is 6.0mm in diameter x 0.3mm in height. Therefore, the volume of the internal space is about 8.48mm 3 =8.48μL. On the bottom surface of the space, there is a Figure 7 The structure shown has three electrodes of roughly the same structure.

[0107] On the other hand, the linear flow channel has an internal space of length 20.5 mm×width 3.0 mm×height 0.3 mm. Therefore, the volume of the internal space is about 18.45 mm 3=18.45μL. On the bottom surface of the space, there is a Fig. 9 The structure shown has three electrodes of roughly the same structure.

[0108] An enzyme membrane having fructosyl amino acid oxidase (FAOD) immobilized by cross-linking with bovine serum albumin (BSA) is formed on the electrode.

[0109] First, prepare a HEPES solution (10mM HEPES, 150mM NaCl, a trace amount of preservatives, pH8.0). Prepare a solution in which 14μM to 15μM FK (fructosyl lysine) is dissolved in the HEPES solution as a measurement solution. Fill each flow channel with the HEPES solution and adjust the state of the enzyme membrane for measurement. Thereafter, introduce the measurement solution into each flow channel device in multiple amounts (50μL to 1000μL) through a pipette operation. At this time, measure the time-dependent change of the current output from the electrode. In the figure shown below, the current value after a specified time (about 120 seconds) from the introduction of the solution is used.

[0110] exist Fig.10 The current value (vertical axis) obtained in each device relative to the amount of solution (horizontal axis) is shown in FIG. Fig.10 In the experiment, the current value was normalized by taking the value at 1000 μL (1 mL) as 100%.

[0111] In the linear flow channel, as the volume decreases, especially below 400 μL, the output decreases significantly. That is, it is recognized that the output decreases at low volumes. On the other hand, in the disc-shaped flow channel, a decrease in output is observed below 200 μL, but even at 50 μL, the output decrease is about 7%. For the disc-shaped flow channel, the problem of a significant decrease in output at low volumes seen in the linear flow channel is not recognized. It can be seen that the disc-shaped flow channel also has high sensing detection performance for low-volume introduced solutions.

[0112] As an explanation, the above results are discussed below. In a linear flow channel, it is believed that the center of the flow channel (the center in the width direction) where the introduced measuring liquid flows most easily from the perspective of fluid mechanics. That is, when a sufficient amount of solution is introduced (for example, 1000 μL), it is believed that the entire volume of the HEPES solution in the flow channel is replaced by the measuring liquid. However, when a sufficient amount of solution is not introduced (for example, less than 400 μL), it is believed that the entire volume of the HEPES solution in the flow channel is not replaced by the measuring liquid. Therefore, the measuring liquid only contacts a portion of the entire area of ​​the electrode. This is believed to lead to a decrease in the measuring current. This situation is believed to be worse as the amount introduced is smaller, which is consistent with Fig.10 In other words, the conversion efficiency of the solution in the linear flow channel is low, while the disc-shaped flow channel has a high conversion efficiency.

[0113] In addition, low solution conversion efficiency refers to the residual of the previous solution. Because this is a phenomenon that occurs when two different fluids come into contact, this may lead to a deviation in the conversion efficiency of the solution, that is, the sensor detection output. In fact, the inventors observed such a deviation in a linear flow channel. On the other hand, the conversion efficiency of a disc-shaped flow channel is high, allowing a certain degree of residual of the previous solution, and it is not easy to cause a deviation in the output.

[0114] As described above, the disk-shaped flow channel has a high solution conversion efficiency for introducing a low volume of measurement solution, and can perform highly sensitive and / or highly stable measurement.

[0115] The above discussion is only an example, and the explanations of the experimental results may be other. The present disclosure does not exclude other scientific and technological explanations, discussions, theories, etc.

[0116] <The influence of the height of the disk-shaped space>

[0117] In this embodiment, the effect of the height of the disc-shaped flow channel on the output current characteristics is studied. A flow channel device with an internal space having a diameter of 6.0 mm and heights of 0.1 mm, 0.28 mm, 0.6 mm, and 1.0 mm is prepared. The bottom surface of the space is provided with a Figure 7 The structure shown is roughly the same as the structure of the three electrodes.

[0118] An enzyme membrane is formed on the electrode, and the enzyme membrane has fructosyl amino acid oxidase (FAOD) fixed by cross-linking with bovine serum albumin (BSA). The enzyme membrane has a thickness of about 25 μm, which is smaller than the accuracy of the height of the internal space. In this way, the four flow channel devices have the same bottom shape and area and the same enzyme membrane, but have different heights of the internal space.

[0119] with Fig.10 A HEPES solution was prepared in the same manner as in the experiment shown in . A solution in which 20 μM FK as a substrate was dissolved in the HEPES solution as a solvent was prepared as a substrate solution. Each flow channel was filled with the HEPES solution to adjust the state of the enzyme membrane for measurement. Thereafter, 100 μL of the substrate solution was introduced into each flow channel device by pipette operation, and at this time, the time-dependent change of the current output from the electrode was measured.

[0120] exist Fig.11 The temporal change of the output current obtained from the introduction of the substrate solution (time t=0) for each flow channel device is shown in FIG. Fig.11An example is shown in each. The device with a height of 0.1mm shows the highest maximum output (about 28nA) and the fastest maximum output arrival time (about 58 seconds), but the maximum output is maintained for a short time. The device with a height of 0.28mm shows a lower maximum output (about 24nA) and a slower maximum output arrival time (about 120 seconds), but the maximum output is maintained for a very long time. The device with a height of 0.6mm and the device with a height of 1.0mm show lower maximum output and slower maximum output arrival time, but the maximum output is maintained longer than that of 0.28mm. Fig.12 : shows the relationship between the height of the disk-shaped space and the holding time of the maximum output.

[0121] exist Fig.11 The device with a height of 0.6 mm shown in the figure shows a slightly lower maximum output current than the device with a height of 1.0 mm. However, no substantial difference was found between the two. In other words, it is estimated that the characteristics are almost unchanged at a height of 0.6 mm or more (see Fig.12 Of course, if the height becomes very large, the tendency of these characteristics may change. For example, the height can be less than 1 mm. The measured volume can be suppressed to be small. In addition, the mixing of bubbles can be suppressed.

[0122] Theoretically, it is believed that a device with a height of 0.1 mm that shows the highest maximum output current has the highest measurement sensitivity. However, the maximum output currents shown by devices with a height of 0.6 mm and devices with a height of 1.0 mm provide sufficient measurement sensitivity for the device disclosed herein. Due to deviations in the timing of introducing the substrate solution into the flow channel device, the liquid delivery speed, etc., deviations occur in the moment of the maximum output current. In other words, maintaining the maximum output current for a long time is beneficial to the stability of the measurement results. For example, the introduction of the substrate solution into the flow channel device can be the starting point for calculating the measurement time. For example, the current value after a certain time from the measurement time of introducing the substrate solution into the flow channel device can also be used as the measurement value. Fig.12 The relationship between the height of the flow channel device and the maximum output holding time is shown in . The time from the time when the maximum output current is supplied to the time when the current value of 1.2 nA, which is the maximum value of the deviation of the current data, is reduced is calculated as the maximum output holding time.

[0123] The device with a height of 0.6 mm and the device with a height of 1.0 mm provide a holding time of the maximum output of about 100 seconds. In reality, since the maximum output current is stable, the error of the calculated holding time is large. In addition, as described above, in this embodiment, there are some differences between the device with a height of 0.6 mm and the device with a height of 1.0 mm, but it can be seen that the same characteristics are shown overall. Therefore, as described above, at a height of 0.6 mm or more, it is estimated that the holding time of the maximum output is almost unchanged ( Fig.12 ).

[0124] On the other hand, the holding time of the maximum output increases approximately linearly from the height of 0.1 mm to 0.6 mm ( Fig.12 ). For example, if the time is 1 minute (60 seconds), the measurement can be stably performed. Since the height of 0.28 mm shows a maximum output holding time of about 60 seconds, it is considered that this is substantially sufficient. Therefore, in several embodiments, the height of the internal space can be 0.28 mm or more. In addition, a height of 0.3 mm can provide a holding time of the maximum output of about 60 seconds. Therefore, in several embodiments, the height of the internal space can be 0.3 mm or more. The height of the internal space can be 0.4 mm, 0.5 mm, etc. or a larger value.

[0125] It should be noted that, in the present embodiment, the thickness of the enzyme membrane is about 25 μm as described above, but the inventors have also confirmed the same tendency when it is 10 μm to 35 μm (not shown). When the thickness of the enzyme membrane is less than 10 μm, the output current value changes sharply and its stability is low. On the other hand, when the thickness of the enzyme membrane is 40 μm, the diffusion of the substrate in the enzyme membrane is slow, and the current measurement efficiency is low.

[0126] The short maximum output time indicates that the current value decreases sharply after the moment of the maximum output current in most cases. The deviation of the characteristics of the enzyme membrane is generated to a certain extent in the manufacturing process. The formation that causes the sharp change of the current may be the main reason for reducing the reproducibility in the measurement. Therefore, the holding time of the maximum output current to a certain extent is at least meaningful for high reproducibility. The long maximum output time indicates that the diffusion / supply speed of the substrate to the enzyme membrane or the concentration gradient of the substrate in the enzyme membrane is constant for a long time. Therefore, high reproducibility can be obtained. In addition, the accuracy of the measured value of the GA value obtained based on the output current curve is also improved.

[0127] There are many methods for calculating the GA value from the output current characteristics (also called the time variation / curve of the current, waveform data, etc.). For example, the current value can be obtained at a specified time starting from the moment when the substrate solution is introduced into the device space. For example, multiple current values ​​on the current curve can be selected or obtained. For example, a statistical value can be obtained from the current curve. For example, a certain statistical value can be obtained from the current values ​​at multiple moments. The GA value can be calculated based on one or more statistical values. For example, the GA value can be obtained based on the maximum output current value and the current value 10 seconds before and after the maximum output current value. For example, the GA value can be obtained based on the area provided by the output current curve. These are illustrative, and other calculation methods can also be used.

[0128] The present disclosure includes the following embodiments:

[0129] A001

[0130] A fluid device comprises: a device body having a disc-shaped space for containing a fluid inside; a fluid inlet configured to introduce the fluid along a tangential direction at a position at 0 o'clock on a substantially circumferential portion of the disc-shaped space; and a fluid outlet configured to discharge the fluid from the space at a position between 6 o'clock and 12 o'clock on a substantially circumferential portion of the disc-shaped space.

[0131] A011

[0132] According to the fluid device described in embodiment A001, the above-mentioned fluid outlet is configured in a manner to guide the above-mentioned fluid out of the above-mentioned space at a position between 7 o'clock and 12 o'clock on a substantially circumferential portion of the above-mentioned disc-shaped space.

[0133] A012

[0134] According to the fluid device described in embodiment A011, the above-mentioned fluid outlet is configured in a manner to discharge the above-mentioned fluid from the above-mentioned space at a position between 9 o'clock and 12 o'clock on a substantially circumferential portion of the above-mentioned disc-shaped space.

[0135] A013

[0136] The fluid device according to any one of Embodiments A001 to A012, wherein the fluid outlet is arranged in the disc-shaped space at a position in the counterclockwise direction (upstream side of the flow of the fluid) relative to the inlet.

[0137] A021

[0138] The fluid device according to any one of Embodiments A001 to A013, wherein the volume of the disk-shaped space is 1 μL to 100 μL.

[0139] A022

[0140] According to the fluid device described in embodiment A021, the volume of the above-mentioned disc-shaped space is 3μL~20μL.

[0141] A023

[0142] According to the fluid device described in embodiment A021, the height of the above-mentioned disc-shaped space is any one of 0.28 mm, 0.3 mm, 0.4 mm, 0.5 mm and 0.6 mm or larger.

[0143] A025

[0144] The fluid device according to any one of Embodiments A001 to A023, wherein the total volume of the disk-shaped space and the volume of the fluid inlet is 2 μL to 200 μL.

[0145] A026

[0146] According to the fluid device described in embodiment A025, the total volume of the above-mentioned disc-shaped space and the volume of the above-mentioned fluid inlet is 6μL~40μL.

[0147] A027

[0148] A fluid device according to any one of embodiments A001 to A026, wherein the volume of the fluid inlet is substantially 50%, 100%, 150% or 200% of the volume of the disc-shaped space.

[0149] A031

[0150] The fluid device according to any one of Embodiments A001 to A027, further comprising a sensor on an inner wall of the disk-shaped space.

[0151] A032

[0152] A fluid device according to embodiment A031, wherein the sensor is a biosensor.

[0153] A033

[0154] A fluid device according to embodiment A031 or A032, wherein the sensor comprises an electrode.

[0155] A034

[0156] A fluid device according to embodiment A033, wherein the electrode comprises a hydrogen peroxide electrode.

[0157] A035

[0158] According to the fluid device described in embodiment A034, the above-mentioned sensor also has an enzyme membrane on the above-mentioned hydrogen peroxide electrode.

[0159] A036

[0160] According to the fluid device described in embodiment A035, the above-mentioned enzyme membrane contains oxidase.

[0161] A037

[0162] According to the fluid device described in embodiment A036, the above-mentioned oxidase is FAOD.

[0163] A038

[0164] The fluid device according to any one of Embodiments A035 to A037, wherein the enzyme membrane contains a protease.

[0165] A039

[0166] According to the fluid device described in embodiment A036, the above-mentioned oxidase is glucose oxidase.

[0167] A041

[0168] A fluid device according to any one of embodiments A031 to A039, wherein the sensor is a protein sensor.

[0169] A042

[0170] A fluid device according to embodiment A041, wherein the sensor comprises a glycated albumin sensor and / or an albumin sensor.

[0171] A051

[0172] A fluid device according to any one of embodiments A031 to A042, wherein the fluid device has an optical sensor outside the disc-shaped space or is configured in combination with an optical sensor outside the disc-shaped space.

[0173] A061

[0174] A fluid device according to any one of embodiments A001 to A051, wherein the fluid device is a stopped-flow fluid device.

[0175] Several embodiments and examples of the present disclosure have been described above, but these embodiments and examples illustrate the present disclosure by way of example. For example, the above-mentioned embodiments are examples that are described in detail in order to easily explain the present disclosure, and the size, structure, material, and circuit may also be modified as needed. It should be noted that the embodiments obtained by arbitrarily combining one or more features of the present disclosure listed above are also included in the scope of the present disclosure. The claims include many variations of the embodiments without departing from the technical idea of ​​the present disclosure. Therefore, the embodiments and examples disclosed in this specification are shown for illustration and should not be considered to limit the scope of the present disclosure.

Claims

1. A fluid device, comprising: A device body having a disc-shaped space inside for containing a fluid; a fluid inlet configured to introduce the fluid in a tangential direction at a position of 0 o'clock on a substantially circumferential portion of the disc-shaped space; and The fluid outlet is configured to guide the fluid out of the disk-shaped space at a position between 6 o'clock and 12 o'clock on a substantially circumferential portion of the disk-shaped space.

2. The fluid device according to claim 1, wherein: The fluid outlet is configured to guide the fluid out of the disk-shaped space at a position between 7 o'clock and 12 o'clock substantially on the circumference of the disk-shaped space.

3. The fluid device according to claim 2, wherein: The fluid outlet is configured to guide the fluid out of the disk-shaped space at a position between 9 o'clock and 12 o'clock substantially on the circumference of the disk-shaped space.

4. The fluid device according to claim 1, wherein: The fluid outlet is arranged at a position in the disk-shaped space in the counterclockwise direction relative to the inlet (on the upstream side of the flow of the fluid).

5. The fluid device according to claim 1, wherein: The volume of the disc-shaped space is 1 μL to 100 μL.

6. The fluid device according to claim 5, wherein: The height of the disc-shaped space is greater than 0.3 mm.

7. The fluid device according to claim 1, wherein: The total volume of the disk-shaped space and the volume of the fluid inlet is 2 μL to 200 μL.

8. The fluid device according to claim 1, wherein: The volume of the fluid inlet is substantially 50%, 100%, 150% or 200% of the volume of the disc-shaped space.

9. The fluid device according to claim 1, wherein: A sensor is also provided on the inner wall of the disc-shaped space.

10. The fluid device according to claim 9, wherein: The sensor is a biosensor.

11. The fluid device according to claim 10, wherein: The electrode comprises a hydrogen peroxide electrode.

12. The fluid device according to claim 11, wherein: The sensor also has an enzyme membrane on the hydrogen peroxide electrode.

13. The fluid device according to claim 12, wherein: The enzyme membrane contains FAOD and protease.

14. The fluid device according to claim 12 or 13, wherein: The enzyme membrane contains glucose oxidase.

15. The fluid device according to claim 10, wherein: The sensor comprises a glycated albumin sensor and / or an albumin sensor. 16 . The fluid device according to claim 1 , comprising an optical sensor outside the disk-shaped space or being configured in combination with an optical sensor outside the disk-shaped space.

17. The fluidic device according to claim 1, which is a stopped-flow fluidic device.

Images