Composition for regulating neurotransmitter and reducing pressure, functional liposome as well as preparation and application of functional liposome

Abstract

The invention relates to a composition for regulating neurotransmitter and reducing pressure, a functional liposome as well as preparation and application of the functional liposome. The functional liposome comprises a nasturtium officinale extract and a rosemary extract in a mass ratio of (0.1-20): (0.1-20). Compared with the prior art, the deep regulation and control of the nasturtium officinale on a neurotransmitter system and the anti-oxidation and anti-inflammatory characteristics of the rosemary are combined, and the solution not only solves the fundamental problem of skin barrier damage, but also improves the overall health level of the skin from multiple aspects. The skin state is comprehensively optimized from reduction of generation of dynamic wrinkles, enhancement of resistance of skin to external stimulation, brightening of skin color and recovery of skin luster.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a composition containing neurotransmitters for regulating and reducing stress, functional liposomes, and preparation and application thereof. Background Art

[0002] In the fast-paced, high-pressure modern life, the human body is in a state of stress for a long time, which not only affects the psychological state, but also causes significant damage to the skin barrier function. Stress can interfere with the normal metabolism and repair process of the skin, leading to weakened skin barrier function, increased water loss, and reduced skin resistance to external stimuli. This change in barrier function makes the skin more vulnerable to external factors such as ultraviolet rays and pollutants, which in turn triggers inflammatory reactions and oxidative stress, accelerating the skin aging process.

[0003] When the skin barrier is damaged, moisture and nutrients on the skin surface are easily lost, causing the skin to lose its luster and become dull. In addition, the inflammatory response will stimulate melanocytes to produce too much melanin, further deepening the dark skin tone.

[0004] Many existing technologies only repair the skin barrier and brighten the skin by temporarily increasing skin hydration and nutrients, or inhibiting inflammatory responses. They do not link the neurotransmitter system with skin health, lack regulation of this key physiological process, and are unable to fully address the complex problems of damaged skin barrier and dull skin tone.

[0005] Therefore, the current industry urgently needs a deep regulation mechanism to optimize the skin condition from the root, effectively slow down the formation of dynamic wrinkles, and improve the overall health of the skin.

[0006] Patent application CN118161441A discloses a skin care composition and a moisturizing cream containing the composition and a preparation method thereof. A skin care composition, in terms of mass percentage, comprises the following components: 1-5% artichoke leaf extract, 1-5% cardamom extract, 1-5% white truffle extract, 1-5% watercress leaf extract, 1-5% ginkgo mistletoe fruit extract, 25-35% moisturizer A, and water is added to the balance to 100%. It can provide long-lasting moisturizing for sensitive skin and relieve discomfort such as itching. However, the patent mainly focuses on improving skin moisturizing, anti-inflammatory and tolerance through plant extracts, but the technology does not consider the in-depth regulation of skin barrier integrity and water retention capacity at the genetic level. At the same time, the technology does not consider the regulation of the neurotransmitter system. Summary of the invention

[0007] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide a composition containing neurotransmitters for regulating and reducing stress, functional liposomes and their preparation and application, aiming to comprehensively combat the damage to the skin barrier caused by stress in modern life and the various skin problems caused thereby.

[0008] The purpose of the present invention can be achieved through the following technical scheme: a composition for regulating neurotransmitters and reducing stress, comprising watercress extract and rosemary extract in a mass ratio of (0.1-20): (0.1-20).

[0009] Furthermore, the composition for regulating neurotransmitters and reducing stress comprises watercress extract and rosemary extract in a mass ratio of (0.1-15):(0.1-10).

[0010] Furthermore, the mass ratio of the watercress extract to the rosemary extract is: (1-3): (1-2).

[0011] The present invention also provides a functional liposome, which contains the composition for regulating neurotransmitters and reducing stress.

[0012] The present invention also provides a method for preparing functional liposomes, comprising the following steps:

[0013] 1) Dissolve the phospholipids in ethanol, stir until fully dissolved, and then transfer to a rotary evaporator to remove the ethanol by low-temperature evaporation to form a uniform phospholipid film on the bottom surface of the container;

[0014] 2) stirring and mixing the phosphate buffer solution, the watercress extract and the rosemary extract;

[0015] 3) adding the mixed solution obtained in step 2) into the rotary evaporator in step 1), performing water treatment (i.e. stirring and ultrasonic treatment) on the phospholipid film, and after the phospholipid film is completely detached, ultrasonic treatment is performed for 3-5 minutes to obtain functional liposomes.

[0016] Furthermore, in step 1), the mass volume ratio of phospholipid to ethanol is (1-5): (5-50) g / mL;

[0017] In step 2), the mass volume ratio of the watercress extract to the phosphate buffer solution is (100-500): (20-50) g / mL;

[0018] The mass volume ratio of rosemary extract to phosphate buffer solution is (100-500): (20-50) mg / mL.

[0019] Furthermore, the phospholipid is selected from soybean lecithin, egg phospholipids, and cephalin;

[0020] Step 3) The particle size of the obtained functional liposomes is between 0.1 and 1 micron.

[0021] The present invention also provides an application of the functional liposome, wherein the functional liposome is used in cosmetics, and the cosmetics contain 1.0-45wt% of the functional liposome.

[0022] Furthermore, the cosmetic contains 2.0-25wt% of the functional liposome.

[0023] Furthermore, the cosmetics include essence, lotion, cream, freeze-dried powder or skin care water.

[0024] Furthermore, the cosmetics also include auxiliary materials, and the auxiliary materials include the following components in percentage by weight:

[0025] Emollients 5.0-35%;

[0026] Emulsifier 0.5-30%;

[0027] Thickener 0.1-20%;

[0028] Preservatives 0.1-2%;

[0029] pH adjuster 0.05-5%;

[0030] Daily fragrance 0.01-5%.

[0031] Further, the emollient is selected from one or more of shea butter, jojoba oil, glycerin, olive oil, sodium hyaluronate, isohexadecane, avocado butter, allantoin, panthenol, butylene glycol, hexylene glycol, squalane, lactic acid, tocopherol, ceramide, hyaluronic acid or sodium lactate;

[0032] The emulsifier is selected from one or more of polysorbate-20, polysorbate-80, glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl trimethyl ammonium methyl sulfate, cocoglyceryl esters or sorbitan olivate;

[0033] The thickener is selected from one or more of hydroxyethyl cellulose, polyethylene, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide, xanthan gum, propylene glycol, behenyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol or gum arabic;

[0034] The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, pentanediol and hexylene glycol;

[0035] The pH regulator is selected from one or more of arginine and lactic acid.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] 1. The present invention provides a dual-effect synergistic skin care strategy. On the one hand, the strategy uses the mineral components of watercress to precisely regulate the neurotransmitter system and skin health homeostasis, promote muscle relaxation, reduce the formation of dynamic wrinkles, and enhance the integrity and moisture retention capacity of the skin barrier through gene-level regulation; on the other hand, with the help of the rich antioxidant mixture in rosemary, free radicals are effectively neutralized, skin oxidative damage is slowed down, bacterial growth is inhibited, and deep-level nourishment and protection are provided to the skin to keep the skin young and healthy.

[0038] 2. The minerals in the watercress of the present invention activate the oxytocin and endocannabinoid pathways in the skin and simultaneously carry out molecular regulation mechanisms, thereby achieving effective management of the skin neurotransmitter system, thereby reducing the negative impact of stress on the skin, regulating neurotransmitters, achieving a decompression effect, thereby improving skin homeostasis, reducing signs of aging, and improving the overall health of the skin. At the same time, it is combined with rosemary to reduce fine lines and wrinkles, inhibit the growth of bacteria on the skin surface, reduce skin inflammation and infection caused by bacteria, and provide a natural protective barrier for the skin. The two work synergistically to promote the synthesis and release of healthy mediators, exert multiple functions such as muscle relaxation and epidermal maintenance, and provide comprehensive stress protection and nourishing support for the skin.

[0039] 3. The present invention combines the deep regulation of the neurotransmitter system by watercress with the antioxidant and anti-inflammatory properties of rosemary. This solution not only solves the fundamental problem of skin barrier damage, but also improves the overall health of the skin from multiple levels. From reducing the formation of dynamic wrinkles, to enhancing the skin's resistance to external stimuli, to brightening the skin tone and restoring skin luster, it achieves a comprehensive optimization of the skin condition. DETAILED DESCRIPTION

[0040] The present invention is described in detail below with reference to specific embodiments.

[0041] The present invention uses a specific ratio of hydrolyzed yeast protein wrap, rice fermentation liquid, and sodium L-lactate to work synergistically, and by adjusting the internal mechanisms such as the biological clock, by simulating or enhancing the natural circadian rhythm of the skin, optimizes its physiological functions, thereby delaying skin aging, allowing the skin to repair and regenerate at the right time, and thus achieving a more lasting anti-aging effect. The specific description is as follows:

[0042] 1. Watercress

[0043] The mineral components in watercress can effectively alleviate the negative effects of stress on the skin barrier function and reduce the occurrence of skin problems such as dull complexion.

[0044] Watercress is rich in various minerals (including magnesium ion Mg2+, manganese ion Mn2+, calcium ion Ca 2 + and potassium ions K+), through their complex molecular mechanisms, precisely regulate the body's neurotransmitter system and skin health homeostasis. In particular, magnesium ions, as a key component, significantly affect the muscle relaxation process, which is an important mechanism for reducing the formation of dynamic wrinkles (such as smile lines and crow's feet). Magnesium ions reduce neuronal excitability by regulating the synthesis and release of neurotransmitters, thereby reducing muscle contractions caused by excessive facial expression activities, promoting skin smoothness, and reducing wrinkle formation. In addition, during the critical period of muscle recovery at night, magnesium ions also assist in ATP synthesis, providing energy for muscle recovery and further preventing the deepening of wrinkles.

[0045] At the same time, calcium ions and manganese ions play a synergistic role in maintaining muscle function and cell metabolism. Although calcium ions dominate muscle contraction, they are also essential for the balance of neuromuscular excitability; while manganese ions support the muscle relaxation process by regulating enzyme activity, promoting cell metabolism and energy production.

[0046] Furthermore, the mineral components in watercress have a significant impact at the genetic level. As key regulators of signal transduction, they enhance the expression and function of neurotransmitter receptors (such as OXTR and CB1R). By promoting the efficient expression of OXTR receptors, strengthening the conduction of the oxytocin (OXT) signaling pathway, increasing the synthesis rate of oxytocin, promoting the health and repair of keratinocytes, optimizing the integrity of the skin barrier, and improving the skin's ability to retain moisture, the skin is naturally radiant and vibrant.

[0047] Furthermore, watercress can help regulate the stress response center in the brain by regulating the expression and function of CB1R receptors and activating the endocannabinoid system (ECS), thereby reducing stress perception and overreaction. The activation of ECS not only fights against skin inflammation and oxidative stress caused by stress, but also promotes the proliferation and differentiation of epidermal cells, strengthens the functionality of the skin barrier, and provides deep nourishment and protection for the skin.

[0048] The watercress extract used in the present invention is a commercially available product.

[0049] 2 rosemary

[0050] Rosemary is rich in a complex and highly effective antioxidant mixture, especially diterpenoid phenolic compounds such as carnosic acid, which can neutralize free radicals and slow down the oxidative damage caused by environmental stress (such as ultraviolet rays, pollution, etc.) to skin cells. This antioxidant mechanism helps reduce the formation of fine lines and wrinkles and keep the skin youthful. These ingredients not only have significant antioxidant activity, but also show strong antimicrobial properties, which help inhibit the growth of bacteria on the skin surface, reduce skin inflammation and infection caused by bacteria, and provide a natural protective barrier for the skin.

[0051] Rosemary extract is used as an effective antioxidant ingredient to prevent oxidation of unsaturated oils, thereby maintaining product stability and extending shelf life. At the same time, it also has a moisturizing effect. Rosemary antioxidant has a slight and almost odorless rosemary smell and can be diluted to no effect in the final product.

[0052] The rosemary extract used in the present invention is a commercially available product.

[0053] 3. Synergy

[0054] The present invention uses a specific mass ratio of (0.1-20): (0.1-20) of watercress extract and rosemary extract to work synergistically, combining the deep regulation of the neurotransmitter system by watercress with the antioxidant and anti-inflammatory properties of rosemary. This solution not only solves the fundamental problem of skin barrier damage, but also improves the overall health of the skin from multiple levels. From reducing the formation of dynamic wrinkles, to enhancing the skin's resistance to external stimuli, to brightening the skin tone and restoring skin luster, it achieves a comprehensive optimization of the skin condition.

[0055] The following is a detailed description through specific examples. The raw materials and detection methods involved in the present invention are commonly used raw materials and detection methods in the art unless otherwise specified.

[0056] Examples 1-3 and Comparative Examples 1-7

[0057] The functional liposomes containing neurotransmitters for regulating and reducing stress include the components in percentage by weight as shown in the following Table 1:

[0058] Table 1 Formulations of Examples 1-3 and Comparative Examples 1-3

[0059]

[0060]

[0061] The above-mentioned liposomes with the function of regulating neurotransmitters and reducing stress are prepared by the following method:

[0062] Dissolve lecithin in ethanol, stir until fully dissolved, and then transfer to a rotary evaporator. In a water bath at 65°C, allow the ethanol to evaporate slowly to form a uniform lecithin film on the container wall.

[0063] The phosphate buffer solution, watercress extract and rosemary extract are stirred and mixed, and the obtained mixed solution is transferred to the container of a rotary evaporator to hydrate the lecithin film for 20 minutes. After the lecithin film is completely detached, ultrasound is performed for 3 minutes to obtain liposomes with the function of regulating neurotransmitters and reducing stress; the particle size of the liposome is 0.1-1 micron.

[0064] The functional liposomes of the present invention can be used in various skin care products, such as lotions, creams, freeze-dried powders, skin care water, etc. The following experiments are set up using essence as an example, but are not limited to the use of essence. The essence is the above key combination and emollients, thickeners, emulsifiers and pH regulators. Preferably, the raw materials for preparing the essence composition containing the functional liposomes for regulating neurotransmitters and reducing stress also include any one or a combination of at least two of preservatives and flavors.

[0065] Application Examples 1-5 and Application Comparative Examples 1-3

[0066] The essence containing liposomes that regulate neurotransmitters and reduce stress has a composition ratio of raw materials as shown in Table 2 below.

[0067] Table 2 Mass percentage of each component used in application examples 1-5 and comparative examples 1-3

[0068]

[0069]

[0070] The essences of the above application examples and comparative application examples are prepared by the following method:

[0071] 1) Water phase: Place water in a container with a stirring device and stir. After fully wetting and mixing the other parts of the phase A raw materials, add them into the water, stir thoroughly until completely dissolved, and heat to 75-80°C.

[0072] 2) Oil phase: Mix the raw materials of phase B and heat to 75-80°C, stirring until completely dissolved.

[0073] 3) Emulsification: Add the mixture obtained in step 2) to the aqueous phase in step 1), stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes.

[0074] 4) Add Phase C raw materials, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes, and cool to 45°C while stirring.

[0075] 5) After cooling to 35-38°C, add the components of phase D, stir at 250-350 rpm and homogenize at 2500-3500 rpm for 3-10 minutes to obtain the required essence. Filter and sterilize before filling, and test the bacterial content, toxins, immunogenicity, etc. for quality control. Stir and cool to 35-38°C to obtain the required essence.

[0076] It should be noted that the above steps all require strict control of sterility.

[0077] Effect test

[0078] (I) Cell proliferation effect test

[0079] Test method:

[0080] 1) Immortalized human epidermal cells (HaCat) were cultured at 10 3 -10 4 The cells were seeded in a 96-well plate at a density of 10 cells / well, 100 μL of culture medium was added, and the cells were cultured in a CO2 incubator at 37°C for 24 hours.

[0081] 2) The functional liposomes of Examples 1-3 and Comparative Examples 1-3 were added to the plate and incubated in an incubator for 48 hours.

[0082] 3) Use a repeating pipette to add 10 μL of CCK8 solution to each well of the culture plate and incubate the culture plate in an incubator for 1-4 hours.

[0083] 4) Use an ELISA reader to measure the absorbance at 450 nm and create a standard curve for data analysis.

[0084] 5) Using Comparative Example 3 as a reference, calculate the cell proliferation change rate, cell proliferation change rate (%) = (absorbance of the test group - absorbance of Comparative Example 3) / absorbance of Comparative Example 3 * 100%. The test results are shown in Table 3 below.

[0085] Table 3 Cell proliferation rate change

[0086]

[0087]

[0088] As shown in Table 3, after cell culture using the functional liposomes of Examples 1-3 and Comparative Example 1, the cell proliferation rate is significantly improved. However, compared with the application of Comparative Example 1, the improvement effect of Examples 1-3 is more significant. From the comparison of the results, it can be seen that the watercress extract has the effect of promoting cell proliferation. Compared with not using liposomes containing the function of regulating neurotransmitters and reducing stress, or only using one of the watercress extract and rosemary extract, when the watercress extract and rosemary extract are used at the same time, the cell proliferation rate improvement effect is more obvious. It shows that the half watercress extract and the rosemary extract have a synergistic effect, which has the effect of improving cell viability and promoting cell proliferation.

[0089] (II) Antioxidant effect test

[0090] Cell ROS inhibition experiment, the specific steps are as follows:

[0091] (1) Cell seeding: Human keratinocytes HaCat were seeded at 2-3×10 5 The cells were inoculated into the wells of a 24-well culture plate at a density of 1.5 cells / well and then placed in a CO2 cell culture incubator for 24 hours.

[0092] (2) According to the experimental grouping, the test substance was added to the cells after inoculation in step (1) and cultured for 24 hours. The experimental groups were as follows: blank control group (medium without serum, no UVB irradiation), injury model group (medium without serum, UVB irradiation), positive control group (vitamin C 200 ug / mL), Example 1-3 and Comparative Example 1-2 (the functional liposomes prepared in Example 1-3 and Comparative Example 1-2 were added to serum-free medium to prepare a sample solution of 200 ug / mL).

[0093] (3) Except for the blank control group, the remaining cells were irradiated with UVB (2 J / cm 2 ).

[0094] (4) The culture medium was discarded, the cells were washed and fixed, and the working concentration of the reactive oxygen species fluorescent probe DCFH-DA reagent was added to the cells for co-incubation according to the reagent operating instructions.

[0095] (5) After incubation, the cells were photographed under an inverted fluorescence microscope and the fluorescence intensity was counted.

[0096] The results show that after UVB irradiation, the intracellular fluorescence intensity increased significantly. Compared with the blank control group, the DCF fluorescence intensity of the damage model group increased to 241.09%, indicating that the UVB light damage model was successfully established; the positive control vitamin C and Examples 1-3 were added in advance, and then UVB irradiation was performed. The results showed that vitamin C had a significant effect on reducing intracellular fluorescence, and the fluorescence was almost invisible. After adding Examples 1-3, the fluorescence intensity decreased by 218.56%, 225.72%, and 203.84%, respectively; after adding Comparative Examples 1-2, the fluorescence intensity decreased by 30.75% and 179.62%, respectively. It shows that Examples 1-3 all have strong antioxidant and anti-photodamage effects. From the comparison results, it can be seen that rosemary extract has antioxidant effects. Compared with not using liposomes containing neurotransmitter regulation and stress reduction effects, or only using one of watercress extract and rosemary extract, when watercress extract and rosemary extract are used at the same time, the antioxidant and anti-photodamage effects are more obvious. It shows that the extract of half-pearl and the extract of rosemary have a synergistic effect and can enhance the effects of cell antioxidant and anti-photodamage.

[0097] (III) Test for promoting oxytocin synthesis

[0098] 1) Immortalized human epidermal cells (HaCat) were cultured at 10 3 -10 4 The cells were seeded at a density of 10 cells / well in a 24-well plate, 100 μL of culture medium was added, and the cells were cultured at 37°C in a CO2 incubator for 24 hours.

[0099] 2) The functional liposome samples prepared in Examples 1-3 and Comparative Examples 1-3 were added to the plate and incubated in an incubator for 48 hours.

[0100] 3) Collect the cell culture supernatant, and use an ELISA kit to add the supernatant of Examples 1-3 and Comparative Examples 1-3 to the corresponding reaction wells in an ELISA plate.

[0101] 4) Use an ELISA reader to measure the absorbance at 450 nm and create a standard curve for data analysis.

[0102] 5) Using Comparative Example 3 as a reference, calculate the oxytocin change rate, oxytocin change rate (%) = (absorbance of the test group - absorbance of Comparative Example 3) / absorbance of Comparative Example 3 * 100%. The test results are shown in Table 4 below.

[0103] Table 4 Oxytocin change rate

[0104] sample Oxytocin change rate % Example 1 117.53 Example 2 122.49 Example 3 103.72 Comparative Example 1 89.14 Comparative Example 2 4.31 Comparative Example 3 0.00

[0105] As shown in Table 4, after cell culture using the functional liposomes of Examples 1-3 and Comparative Examples 1-3, the oxytocin content was significantly improved. However, compared with Comparative Example 1, the improvement effect of Example 1-3 is more significant. As can be seen from the comparison of the results, the watercress extract has the effect of promoting the synthesis of oxytocin. Compared with not using liposomes containing the function of regulating neurotransmitters and reducing stress, or only using one of the watercress extract and rosemary extract, when the watercress extract and rosemary extract are used at the same time, the oxytocin content improvement effect is more obvious. It shows that the half watercress extract and the rosemary extract have a synergistic effect, which has the effect of promoting the synthesis of oxytocin and making people feel happy.

[0106] (IV) Safety testing

[0107] 1) Test samples: Application Examples 1-5 and Application Comparative Examples 1-3.

[0108] 2) Number of participants: 90 participants, half male and half female, aged between 20 and 55 years old, all meeting the criteria for subjects.

[0109] 3) Test method: 90 subjects who meet the test criteria were randomly divided into 9 groups, corresponding to the essences obtained from application examples 1-5 and comparative examples 1-3 described by the test subjects, and blank group 1 was a group that did not apply any sample, with 10 subjects in each group; a third-party testing agency was used to test safety. The selected area should not exceed 50mm 2 , a suitable patch with a depth of about 1mm, add about 0.02-0.025ml (g) of the test substance into the patch tester by closed patch test method. Stick the patch tester on the curved side of the subject's forearm, remove the test substance after 24 hours, and observe the skin reaction at 0.5h, 24h, and 48h after removal: record the results according to the skin reaction grading standards in the "Cosmetic Safety Technical Regulations 2015".

[0110] Table 5 Patch test results

[0111]

[0112]

[0113] Note: Skin reaction grading standards:

[0114] Grade 0: negative reaction;

[0115] Grade 1: Suspicious reaction; only slight erythema;

[0116] Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0117] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes: the reaction may extend beyond the tested area;

[0118] Grade 4: Very strong reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0119] 4) Test results: The results in Table 3 show no irritation and are considered safe.

[0120] (V) Stability test

[0121] 1) Test sample: the essence in Application Examples 1-5.

[0122] 2) Test method: Internal control standard, observe the appearance color, viscosity and pH for three months.

[0123] Table 6 Stability test results

[0124] time 45℃ 40℃ 37℃ 25℃ 5℃ -15℃ CYCLE illumination 1W √ √ √ √ √ √ √ √ 2W √ √ √ √ √ √ √ √ 3W √ √ √ √ √ √ √ √ 4W √ √ √ √ √ √ √ √ 2M √ √ √ √ √ √ √ √ 3M √ √ √ √ √ √ √ √

[0125] Among them, W stands for week, M stands for month, and "CYCLE" refers to cycle. The cycle setting is: heating from -15℃ to 45℃, and cooling from 45℃ to -15℃ for five times.

[0126] 3) Test results: The results in Table 6 show that the essences in Application Examples 1-5 have stable properties within 3 months.

[0127] (VI) Test of moisture content of stratum corneum

[0128] 1) Test sample: the essence prepared in Application Examples 1-5 and Comparative Examples 1-3.

[0129] 2) Test subjects: 80 healthy women (23-45 years old, average age 34.6 years old) were randomly divided into 8 groups, each with 10 people, regardless of gender, and each group used the same formula product. Written informed consent was signed. Before enrollment, a series of questions about medical history and health status were asked according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0130] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0131] 3) Test method: Use the sample twice a day, morning and evening, for 13 weeks. Apply an appropriate amount of the test sample around one eye of the subject, and do not use the test sample around the other eye. Apply sunscreen products to the entire face every morning.

[0132] The test results were collected at the following four time periods: before using the sample (D0), 7 days after using the sample (D7), 14 days after using the sample (D14), and 91 days after using the sample (D91).

[0133] 4) Testing equipment: Corneometer CM825 is used to measure the moisture content of the stratum corneum. The higher the measured value, the higher the moisture content of the stratum corneum of the skin.

[0134] 5) The test results can be calculated using the following formula:

[0135] Change rate = (average analysis value of 10 people in each group after using the product - average analysis value of 10 people in each group before using the product) ÷ average analysis value of 10 people in each group before using the product × 100%

[0136] Table 7 Change rate of moisture content in stratum corneum

[0137]

[0138]

[0139] As can be seen from the results in Table 7, after using the essence, as the use time increases, the change rate of the water content of the stratum corneum of the skin of Application Examples 1-5 and Application Comparative Examples 1-3 increases, indicating that the water content of the stratum corneum of the skin has improved. However, compared with Application Comparative Examples 1-3, when used for the same time, the change rate of the water content of the stratum corneum of Application Examples 1-5 is greater, indicating that the improvement effect of the water content of the stratum corneum of the skin after using the essence of Application Examples 1-5 is more significant.

[0140] From the comparison of the results of application examples 1-5 and comparative examples 1-3, it can be seen that when the watercress extract and rosemary extract are used together, the water content of the stratum corneum of the skin is improved better than when the essence composition containing liposomes with the effect of regulating neurotransmitters and reducing stress is not used, or when only one of the watercress extract and rosemary extract is used. This shows that the watercress extract and the rosemary extract have a synergistic effect and can effectively improve the water content of the stratum corneum of the skin.

[0141] (VII)TEWL(g / m 2 / h) Transepidermal water loss test

[0142] 1) Test sample: the essence prepared in Application Examples 1-5 and Comparative Examples 1-3.

[0143] 2) Test subjects: 80 healthy women (23-45 years old, average age 34.6 years old) were randomly divided into 8 groups, each with 10 people, regardless of gender, and each group used the same formula product. Written informed consent was signed. Before enrollment, a series of questions about medical history and health status were asked according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0144] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0145] 3) Test method: Use the sample twice a day, morning and evening, for 13 weeks. Apply an appropriate amount of the test sample on one cheek of the subject, and do not use the test sample on the other cheek. Apply sunscreen products to the entire face every morning.

[0146] The test results were collected at the following four time periods: before using the sample (D0), 7 days after using the sample (D7), 14 days after using the sample (D14), and 91 days after using the sample (D91).

[0147] 4) Testing equipment: Use the non-invasive measuring device TEWL (Dermalab Combo) to accurately measure the transepidermal water loss (TEWL). If the TEWL decreases significantly before and after the use of the test product, it means that the skin barrier has been restored and the product has a repairing effect.

[0148] 5) The test results can be calculated using the following formula:

[0149] Change rate = (average analysis value of 10 people in each group after using the product - average analysis value of 10 people in each group before using the product) ÷ average analysis value of 10 people in each group before using the product × 100%

[0150] Table 8 Change rate of skin transepidermal water loss value

[0151] sample After 7 days of use % After 14 days of use % After 91 days of use % Application Example 1 -13.52 -18.26 -27.31 Application Example 2 -14.86 -19.79 -30.25 Application Example 3 -11.88 -16.26 -24.92 Application Example 4 -16.17 -20.95 -31.68 Application Example 5 -10.24 -15.11 -22.83 Application Comparative Example 1 -7.47 -10.83 -18.27 Application Comparative Example 2 -7.02 -10.15 -16.93 Application Comparative Example 3 -4.34 -6.18 -9.24

[0152] From the results in Table 8, it can be seen that after using the essence, as the use time increases, the change rate of the transepidermal water loss value of the skin of application examples 1-5 and application comparative examples 1-3 decreases, indicating that the skin barrier is improved. However, compared with comparative examples 1-3, when used for the same time, the change rate of the transepidermal water loss value of the skin of application examples 1-5 is greater, indicating that the skin barrier repair is more significant after using the essence of application examples 1-5.

[0153] From the comparison of the results of application examples 1-5 and comparative examples 1-3, it can be seen that compared with not using the essence composition containing liposomes with the effect of regulating neurotransmitters and reducing stress, or using only one of the watercress extract and rosemary extract, the improvement effect of the transepidermal water loss value of the skin is better when the watercress extract and rosemary extract are used at the same time. This shows that the watercress extract and the rosemary extract have a synergistic effect, can effectively improve the transepidermal water loss value of the skin, and have a barrier repair effect.

[0154] (8) Skin gloss test

[0155] 1) Test sample: the essence prepared in Application Examples 1-5 and Comparative Examples 1-3.

[0156] 2) Test subjects: 80 healthy women (23-45 years old, average age 34.6 years old) were randomly divided into 8 groups, each with 10 people, regardless of gender, and each group used the same formula product. Written informed consent was signed. Before enrollment, a series of questions about medical history and health status were asked according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0157] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0158] 3) Test method: Use the sample twice a day, morning and evening, for 13 weeks. Apply an appropriate amount of the test sample on one cheek of the subject, and do not use the test sample on the other cheek. Apply sunscreen products to the entire face every morning.

[0159] The test results were collected at the following four time periods: before using the sample (D0), 7 days after using the sample (D7), 14 days after using the sample (D14), and 91 days after using the sample (D91).

[0160] 4) Testing equipment: Glossymeter GL200 is used for testing. The higher the measured value, the better the glossiness of the skin.

[0161] 5) The test results can be calculated using the following formula:

[0162] Change rate = (average analysis value of 10 people in each group after using the product - average analysis value of 10 people in each group before using the product) ÷ average analysis value of 10 people in each group before using the product × 100%

[0163] Table 9 Skin glossiness change rate

[0164]

[0165]

[0166] From the results in Table 9, it can be seen that after using the essence, as the use time increases, the glossiness change rate of the skin of Application Examples 1-5 and Application Comparative Examples 1-3 increases, indicating that the dull skin complexion has improved. However, compared with Application Comparative Examples 1-3, when used for the same time, the glossiness change rate of the skin of Application Example 1-5 is greater, indicating that the skin complexion is more significantly dull after using the essence of Application Examples 1-5.

[0167] From the comparison of the results of application examples 1-5 and application comparative examples 1-3, it can be seen that compared with not using the essence composition containing liposomes with the effect of regulating neurotransmitters and reducing stress, or using only one of the watercress extract and the rosemary extract, the effect of improving the glossiness of the skin is better when the watercress extract and the rosemary extract are used at the same time. This shows that the watercress extract and the rosemary extract have a synergistic effect, which can effectively improve the dullness of the skin and increase the glossiness.

[0168] In summary, the present invention combines the synergistic effect of watercress minerals and rosemary antioxidant components, can enhance the effects of cell antioxidant and anti-photodamage, promote oxytocin synthesis, make people happy, can effectively improve the water content of the skin stratum corneum, improve the skin's transepidermal water loss value, has a barrier repair effect, and can effectively improve skin dullness and increase glossiness.

[0169] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A composition for regulating neurotransmitters and reducing stress, characterized in that: The invention comprises a watercress extract and a rosemary extract in a mass ratio of (0.1-20):(0.1-20).

2. A composition for regulating neurotransmitters and reducing stress according to claim 1, characterized in that: The invention comprises a watercress extract and a rosemary extract in a mass ratio of (0.1-15):(0.1-10).

3. A composition for regulating neurotransmitters and reducing stress according to claim 1 or 2, characterized in that: The mass ratio of the watercress extract to the rosemary extract is: (1-3): (1-2).

4. A functional liposome, characterized in that: The functional liposome contains the composition according to claim 1 or 2.

5. A method for preparing the functional liposome as claimed in claim 4, characterized in that: The following steps are involved: 1) Dissolve the phospholipids in ethanol, stir until fully dissolved, and then transfer to a rotary evaporator to remove the ethanol by low-temperature evaporation to form a uniform phospholipid film on the bottom surface of the container; 2) stirring and mixing the phosphate buffer solution, the watercress extract and the rosemary extract; 3) adding the mixed solution obtained in step 2) into the rotary evaporator in step 1), performing water treatment on the phospholipid film, and performing ultrasonic treatment after the phospholipid film is completely detached to obtain functional liposomes.

6. The method for preparing a functional liposome according to claim 5, characterized in that: In step 1), the mass volume ratio of phospholipid to ethanol is (1-5): (5-50) g / mL; In step 2), the mass volume ratio of the watercress extract to the phosphate buffer solution is (100-500): (20-50) mg / mL; The mass volume ratio of rosemary extract to phosphate buffer solution is (100-500): (20-50) mg / mL; The lecithin membrane material is selected from soybean lecithin, egg phospholipids, and cephalin; Step 3) The particle size of the obtained functional liposomes is between 0.1 and 1 micron.

7. A use of the functional liposome as claimed in claim 4, characterized in that: The functional liposome is used in cosmetics, and the cosmetics contain 1.0-45wt% of the functional liposome.

8. The use of the functional liposome according to claim 7, characterized in that: The cosmetic contains 2.0-25 wt % of the functional liposome.

9. The use of the functional liposome according to claim 7 or 8, characterized in that: The cosmetics include essence, emulsion, cream, freeze-dried powder or skin care water.

10. The use of the functional liposome according to claim 7, characterized in that: The cosmetics also include auxiliary materials, which include emollients, emulsifiers, thickeners, preservatives, pH regulators and daily fragrances; The emollient is selected from one or more of shea butter, jojoba oil, glycerin, olive oil, sodium hyaluronate, isohexadecane, avocado butter, allantoin, panthenol, butylene glycol, hexylene glycol, squalane, lactic acid, tocopherol, ceramide, hyaluronic acid or sodium lactate; The emulsifier is selected from one or more of polysorbate-20, polysorbate-80, glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl trimethyl ammonium methyl sulfate, cocoglyceryl esters or sorbitan olivate; The thickener is selected from one or more of hydroxyethyl cellulose, polyethylene, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide, xanthan gum, propylene glycol, behenyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol or gum arabic; The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, pentanediol and hexylene glycol; The pH regulator is selected from one or more of arginine and lactic acid.

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