Intelligent wound dressing for matrix metalloproteinase detection and administration and application

Abstract

The invention discloses an intelligent wound dressing for matrix metalloproteinase detection and administration and application. The intelligent wound dressing comprises biological sensitive hydrogel, an electrode packaging layer, a flexible interdigital electrode, a device packaging layer, a signal transmission antenna and a skin adhesion layer. The bio-sensitive hydrogel is used for biologically recognizing matrix metalloproteinase and carrying and controlled release of antibacterial drugs, and the flexible interdigital electrode and the signal transmission antenna are electrically connected through welding and used for data collection and transmission. The concentration of matrix metalloproteinase in a wound is dynamically detected through biological sensitive hydrogel and a wireless passive radio frequency technology, the inflammation condition of the wound is reflected in real time, silver nanoparticles are delivered according to needs for bacteriostatic treatment, and therefore closed-loop management of the wound is achieved, and the wound healing effect is improved. And a personalized detection and treatment integrated platform is provided for chronic wounds.

Description

Technical Field

[0001] The present invention relates to the technical field of wound inflammation detection and drug administration, and in particular to an intelligent wound dressing for matrix metalloproteinase detection and drug administration and application thereof. Background Art

[0002] Chronic wounds are a major clinical medical problem secondary to social development issues such as population aging, obesity and diabetes. In addition to causing pain, infection, loss of function and even amputation, chronic wounds also place a heavy burden on patients and their families. In clinical practice, wounds are usually treated as complications of other diseases and are easily neglected in clinical treatment. Clinicians are usually not highly specialized in the diagnosis and treatment of chronic wounds, resulting in a lack of effective allocation of chronic wound care resources, inconsistent clinical opinions, and prolonged wound healing time. Therefore, chronic wound management is an important and neglected area.

[0003] Traditional chronic wound assessment methods are mainly based on visual observation and clinical experience, which are highly subjective, easy to draw inaccurate conclusions, and cannot meet the needs of personalized wound management. Accurate laboratory analysis in clinical practice relies on blood analysis as the gold standard, which not only requires complex sample preparation, large equipment and the participation of professionals, but also inevitably brings discomfort and infection risks to the wound. It is discontinuous and sporadic, so it cannot provide timely and effective feedback on the healing of chronic wounds. At present, some sensors have been used to detect physiological and chemical parameters such as pH, glucose and temperature as indicators of wound healing process, but the information provided is limited and only stops at wound detection, which cannot effectively meet the clinical needs of closed-loop detection and treatment. Therefore, it is of great significance to develop an in-situ analysis that can quantify the wound status at the wound site, accurately diagnose the healing of chronic wounds, and at the same time, through the controlled release of antibacterial drugs, the wound is given on demand. The smart wound dressing is of great significance for the initial diagnosis, personalized management and wound healing of chronic wounds. Summary of the invention

[0004] The purpose of the present invention is to address the deficiencies of the prior art and provide an intelligent wound dressing and application for matrix metalloproteinase detection and drug administration to solve the problem of wireless instant detection and antibacterial treatment of wound inflammation.

[0005] To achieve the above object, the present invention is implemented by the following technical scheme: an intelligent wound dressing for matrix metalloproteinase detection and drug delivery, the intelligent wound dressing comprising from top to bottom:

[0006] A biosensitive hydrogel comprising a bioactive arginyl-glycyl-aspartic acid polypeptide sequence for biorecognition of matrix metalloproteinases in wounds and controlled release of antimicrobial drugs based on the concentration of matrix metalloproteinases;

[0007] Electrode packaging layer;

[0008] Flexible interdigitated electrodes;

[0009] Device packaging layer;

[0010] A signal transmission antenna connected to the flexible interdigital electrodes for data collection and transmission;

[0011] Skin adhesive layer, used for adhesion to the skin.

[0012] Furthermore, the biosensitive hydrogel is prepared by a photocuring method, specifically: 10 mg of methacrylated gelatin is added to 100 mL of PBS buffer containing 2 mg / mL photoinitiator Irgacure 2959, stirred at 37°C until completely dissolved, 100 mL of a silver nanoparticle dispersion with a concentration of 85 mg / mL is added, and mixed evenly to obtain a first solution; 10 to 30 mL of the first solution is added dropwise to the surface of the interdigitated electrode, and cross-linked by 8W, 365 nm ultraviolet light irradiation for 20 minutes.

[0013] Furthermore, the silver nanoparticle dispersion is prepared by an ascorbic acid / citric acid reduction method in the presence of anions, specifically, 2 mL of sodium citrate with a mass concentration of 1%, 0.5 mL of silver nitrate with a mass concentration of 1%, and 0.4 mL of 20 mM sodium chloride are sequentially added to 2.1 mL of water, and stirred for 5 minutes to obtain a second solution; the second solution is added to 94.9 mL of boiling water containing 100 mL of 0.1 M ascorbic acid solution, and boiling and stirring are continued for 1 hour to obtain a silver nanoparticle dispersion.

[0014] Furthermore, the electrode packaging layer is made of epoxy resin material, prepared by spin coating and UV curing, with a thickness of 1 to 5 μm, and covers the interdigital part of the flexible interdigital electrode to ensure that the frequency response signal is obtained at a lower frequency.

[0015] Furthermore, the device packaging layer is made of polydimethylsiloxane material with a thickness of 50 to 200 μm, and is used to package and protect the signal transmission antenna.

[0016] Furthermore, the inductance of the signal transmission antenna is 0.2-0.5 μH.

[0017] Furthermore, the flexible interdigital electrodes and the signal transmission antenna are electrically connected by welding.

[0018] Furthermore, the skin adhesive layer is made of polyurethane.

[0019] Preferably, it also includes a wireless terminal coupled to the signal transmission antenna for wirelessly reading out the chronic wound healing condition detection signal.

[0020] The present invention also provides an application of the above-mentioned smart wound dressing, specifically: the smart wound dressing is attached to a wound for wound matrix metalloproteinase detection and / or drug controlled release.

[0021] The beneficial effects of the present invention are as follows:

[0022] The present invention provides a low-cost smart wound dressing to dynamically detect wound conditions and administer medication on demand. The smart wound dressing dynamically detects the concentration of matrix metalloproteinases through bioresponsive hydrogels and wireless passive radio frequency technology to obtain quantifiable indicators to characterize the wound inflammation state, and is assisted by silver nanoparticle delivery for treatment to form a closed-loop detection and treatment system. By combining radio frequency technology, biosensitive hydrogels and flexible electronic technology, a wireless passive flexible radio frequency sensor device is constructed, which realizes the miniaturization, integration and flexibility of the system, and provides low-cost personalized wound management and treatment solutions for patients with chronic wounds. Therefore, the device and method of the present invention can be widely used in the detection and administration of chronic wound inflammation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application:

[0024] Figure 1 is a schematic diagram of the structure of a smart wound dressing provided by an embodiment of the present invention;

[0025] Figure 2 is a schematic diagram of the preparation process of the biosensitive hydrogel provided in an embodiment of the present invention;

[0026] Figure 3 It is a Fourier transform infrared spectrum characterization diagram of different hydrogel precursors provided in the embodiments of the present invention;

[0027] Figure 4 is a scanning electron microscope image of a biosensitive hydrogel provided in an embodiment of the present invention;

[0028] Figure 5 is a graph showing the relationship between the survival rate of human immortalized keratinocytes (HaCat) and the incubation time provided in an embodiment of the present invention;

[0029] Figure 6 The effects of different concentrations of matrix metalloproteinases on the degradation rate of biosensitive hydrogels provided in the embodiments of the present invention;

[0030] Figure 7 It is a schematic diagram of the application scenario and working principle of the smart wound dressing provided by an embodiment of the present invention;

[0031] Figure 8 It is the resonant frequency response of the smart wound dressing provided by the embodiment of the present invention and the matrix metalloproteinases with different concentrations incubated for 24 hours;

[0032] Fig. 9 is the resonant frequency response of the smart wound dressing provided by the embodiment of the present invention when incubated for 24 hours at a matrix metalloproteinase concentration of 0 to 1000 ng / mL;

[0033] Fig.10 It is a linear fitting curve diagram of the detection of the concentration of matrix metalloproteinases of 0 to 1000 ng / mL of the smart wound dressing provided in the embodiment of the present invention;

[0034] Fig.11 is the resonant frequency response of the smart wound dressing provided by an embodiment of the present invention when incubated for 2 hours at a concentration of 0 to 25 μg / mL of matrix metalloproteinase;

[0035] Fig.12 It is a linear fitting curve diagram of the detection of the concentration of matrix metalloproteinases of 0 to 25 μg / mL of the smart wound dressing provided by the embodiment of the present invention;

[0036] Fig.13 This is an anti-interference test diagram for detecting the concentration of matrix metalloproteinases in a smart wound dressing provided in an embodiment of the present invention;

[0037] Fig.14 This is a stability test diagram of the intelligent wound dressing provided by an embodiment of the present invention;

[0038] Fig.15 is a graph showing the relationship between the release concentration of silver nanoparticles and the incubation time of matrix metalloproteinases in the smart wound dressing provided in an embodiment of the present invention;

[0039] Fig.16 This is a test diagram of the antibacterial performance of the smart wound dressing provided by an embodiment of the present invention;

[0040] Fig.17 This is a graph showing the test results of the concentration of matrix metalloproteinases in wound exudate of patients with diabetic foot ulcers using the smart wound dressing provided in an embodiment of the present invention;

[0041] Fig.18 is a correlation analysis result diagram between the smart wound dressing and fluorescence detection provided by an embodiment of the present invention;

[0042] In the figure: biosensitive hydrogel 1; electrode packaging layer 2; flexible interdigitated electrode 3; device packaging layer 4; signal transmission antenna 5; skin adhesion layer 6. DETAILED DESCRIPTION

[0043] The embodiments, features and aspects of the present invention will be described in detail below with reference to the accompanying drawings, but the present invention is not limited thereto. Any embodiment expanded based on the embodiments of the present invention, without making creative work, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the present invention. The same symbols in the drawings represent the same or functionally similar elements. Although various aspects of the embodiments are shown in the drawings, the drawings do not have to be drawn to scale unless otherwise specified.

[0044] In addition, in order to better illustrate the present invention, numerous specific details are given in the specific implementation modes below. Those skilled in the art should understand that the present invention can also be implemented without certain specific details. Some methods and means well known to those skilled in the art and the use of components are not described in detail in order to highlight the main purpose of the present invention.

[0045] like Figure 1 As shown, an embodiment of the present invention provides an intelligent wound dressing for matrix metalloproteinase detection and drug controlled release, which includes: a biosensitive hydrogel 1, an electrode packaging layer 2, a flexible interdigital electrode 3, a device packaging layer 4, a signal transmission antenna 5, and a skin adhesion layer 6. The biosensitive hydrogel 1 is used for biological recognition of matrix metalloproteinases and the loading and controlled release of antibacterial drugs, and is covered on the electrode packaging layer 2. The electrode packaging layer 2 is made of epoxy resin material, prepared by spin coating and ultraviolet light curing, with a thickness of 1 to 5 μm, and covers the interdigital part of the flexible interdigital electrode 3 to ensure that the frequency response signal is obtained at a lower frequency. The device packaging layer 4 uses polydimethylsiloxane material to encapsulate the signal transmission antenna 5 to form protection. The inductance of the signal transmission antenna 5 is 0.2 to 0.5 μH. The flexible interdigital electrode 3 and the signal transmission antenna 5 are electrically connected by welding to form a flexible inductor-capacitor resonant circuit for data collection and transmission. The skin adhesion layer 6 is located at the bottom of the device, and polyurethane is used as a medical adhesive layer for adhesion between the intelligent wound dressing and the skin.

[0046] In one possible implementation, the signal transmission antenna 5 is prepared by a flexible printed circuit board processing technology and a laser cutting method, and the flexible interdigitated electrode 3 is prepared by laminating a copper metal layer on the surface of a polyethylene terephthalate film, and then using an electroplating process to sequentially deposit a 1-2 μm nickel metal layer and a 1-2 μm thick gold metal layer.

[0047] like Figure 2 As shown, the preparation method of the biosensitive hydrogel 1 comprises the following steps:

[0048] (1) Preparation of silver nanoparticles: Silver nanoparticles were prepared as antibacterial agents by ascorbic acid / citric acid reduction in the presence of anions. 2 mL of sodium citrate (1% wt), 0.5 mL of silver nitrate (1% wt) and 0.4 mL of sodium chloride (20 mM) were added to 2.1 mL of deionized water in sequence and then stirred for 5 minutes. The mixture was added to 94.9 mL of boiling water containing 100 mL of ascorbic acid solution (0.1 M), and boiling and stirring were continued for 1 hour to obtain a yellow silver nanoparticle dispersion.

[0049] (2) Preparation of biosensitive hydrogels

[0050] 10 mg of methacrylated gelatin was added to 100 mL of PBS buffer containing 2 mg / mL photoinitiator Irgacure 2959, stirred at 37°C until completely dissolved, and then 100 mL of 85 mg / mL silver nanoparticle dispersion was added and mixed evenly. 10 to 30 mL of the above solution was dripped onto the surface of the interdigitated electrode and irradiated under ultraviolet light (8 W, 365 nm) for crosslinking for 20 minutes to finally form a biosensitive hydrogel.

[0051] like Figure 3 As shown, the biosensitive hydrogel 1 has a corresponding molecular structure. The molecular structure analysis was performed by Fourier transform infrared spectroscopy. It can be seen from the figure that the position of the characteristic peak has not changed, indicating that the methacryloylated gelatin hydrogel formed by free radical polymerization under ultraviolet light irradiation has not introduced new functional groups. Methacryloylated gelatin (GelMA), methacryloylated gelatin hydrogel (GelMA hydrogel), and methacryloylated gelatin hydrogel doped with silver nanoparticles (GelMA@AgNPs hydrogel) have a peak at 1640 cm -1 (C=O stretching vibration) and 1549 cm -1 The spectrum at (NH bending vibration) is the characteristic peak of gelatin protein.

[0052] like Figure 4 The figure shows a scanning electron microscope characterization image of the smart wound dressing of the present invention. The structure of the hydrogel is observed by a general scanning electron microscope, and a uniform and interconnected three-dimensional pore structure can be seen, indicating the successful synthesis of the polymer.

[0053] like Figure 5 The figure shows the survival rate comparison of HaCat cells in the smart wound dressing of the present invention. In order to test the biocompatibility of the biosensitive hydrogel, the biosensitive hydrogel was incubated with HaCaT cells for 24, 48, and 72 hours, and the cell survival was statistically analyzed. Compared with the control group, there was no significant difference in the relative survival rate of HaCaT cells in the hydrogel group. The survival rates of cells cultured in the sensor and hydrogel degradation solution after incubation for 72 hours were all above 98%.

[0054] like Figure 6 As shown, the effect of different concentrations of matrix metalloproteinase on the degradation rate of biosensitive hydrogel in the smart wound dressing of the present invention is shown. In order to illustrate the degradability of GelMA@AgNPs hydrogel, it was incubated in a matrix metalloproteinase solution. It can be seen from the figure that the degradation rate increases with the increase of the concentration of matrix metalloproteinase. 1000ng / mL matrix metalloproteinase solution can completely degrade the hydrogel within 24h, while the effect of 0ng / mL matrix metalloproteinase on the biosensitive hydrogel can be ignored. It shows that the degradation of biosensitive hydrogel is affected by the concentration of matrix metalloproteinase, so it can be used as a carrier for sensing and drug controlled release.

[0055] The working principle of the smart wound dressing is as follows Figure 7 As shown. The biosensitive hydrogel 1 will produce a specific response to the matrix metalloproteinase in the wound exudate, thereby causing a change in the capacitance in the flexible inductor-capacitor resonant circuit and a controlled release of the antibacterial drug. The wireless readout of the chronic wound healing detection signal is achieved through the coupling of the readout coil and the signal transmission antenna 5. The wireless terminal can be connected to the vector network analyzer to achieve data transmission and complete further processing and analysis.

[0056] The smart wound dressing can generate a continuous signal response to the concentration of matrix metalloproteinases. Figure 8 As shown, different concentrations of matrix metalloproteinase solutions were prepared for the characterization of the sensing performance. Under the same test time, the higher the concentration, the greater the corresponding frequency change, and the lower the resonant frequency of the resonant circuit (such as Fig. 9 The resonant frequency of the sensor changes linearly with the concentration of matrix metalloproteinases, R 2 is 0.9915 (e.g. Fig.10 As shown in the figure, the sensitivity of the sensor to the concentration of matrix metalloproteinases is 0.0090 MHz / (ng / mL), indicating that the sensor has good linearity and can detect low concentrations of matrix metalloproteinases.

[0057] The sensor module of the smart wound dressing has high selectivity for matrix metalloproteinases and is not easily interfered by other substances in the wound. Physiological concentrations of sodium chloride, potassium chloride, glucose, lactic acid, uric acid, creatinine and albumin solutions are prepared for selective characterization. Fig.11 As shown, compared with matrix metalloproteinases, the effects of all interfering substances on the resonance frequency shift are negligible, indicating that the matrix metalloproteinase sensor has good anti-interference ability and can adapt to the detection of complex wound environments from the perspective of selectivity.

[0058] The smart wound dressing has high stability and can be stored in a 37°C buffer for several days without affecting its sensor module. Fig.12 As shown, the sensor was stored in a buffer solution at 37°C and tested repeatedly under the same conditions for 8 days. Its resonance frequency shift signal remained stable within 4 days, and there was a slight resonance frequency shift after 4 days.

[0059] The smart wound dressing of the present invention can realize matrix metalloproteinase detection and antibacterial treatment in chronic wound management. In diabetic foot ulcers, matrix metalloproteinases can degrade the extracellular matrix and delay wound healing. When the concentration of matrix metalloproteinases in diabetic foot ulcers is higher than 3μg / mL, the wound healing is poor. In order to realize the application in the wounds of patients with diabetic foot ulcers, the wound patch is tested and verified in a high-concentration matrix metalloproteinase solution. In one possible implementation method, a matrix metalloproteinase solution with a concentration range of 0 to 25μg / mL is prepared, incubated with the smart wound dressing, and the resonant frequency of the smart wound dressing is detected using a vector network analyzer ( Fig.13 ), with the increase of MMP concentration, the resonance frequency gradually increases. The resonance frequency shift is linearly related to MMP concentration, R 2 is 0.9743( Fig.14 ), the sensitivity of the smart wound dressing to the concentration of matrix metalloproteinases is 0.1236MHz / (μg / mL), which can meet the detection needs of diabetic foot ulcer wounds.

[0060] In order to verify the reliability of the constructed smart wound dressing detection, the wound exudate of patients with diabetic foot ulcers was tested. The concentration of matrix metalloproteinases in the wound was detected using the smart wound dressing, and the results of the matrix metalloproteinase fluorescence detection kit were used as a reference to measure the concentration of matrix metalloproteinases in the same wounds of different patients. The results are shown in Fig.15 As shown in Figure 2, the concentration of matrix metalloproteinases in the wounds of patients with diabetic foot ulcers ranged from 5 μg / mL to 25 μg / mL. Fig.16 As shown), R 2 The value is 0.9429, and the slope is 0.9030, which is close to 1, indicating that the results of the two methods are highly correlated, proving the reliability of smart wound dressing detection.

[0061] The smart wound dressing has the function of controlled drug release, wherein the release of antibacterial drugs is related to the degradation of matrix metalloproteinases, and the release rate is related to the concentration of matrix metalloproteinases, the higher the concentration, the faster the release rate. In order to characterize the difference between the natural release of silver nanoparticle drugs in the hydrogel and the controlled release rate of matrix metalloproteinases, the concentration of silver nanoparticles in the degradation solution was measured, such as Fig.17As shown in the figure, after 48h incubation with matrix metalloproteinases, the hydrogel degradation released 1.75μg / mL silver nanoparticles, while the hydrogel without matrix metalloproteinase incubation released 0.28μg / mL silver nanoparticles. The controlled release rate of silver nanoparticles was higher than the natural release rate, indicating that hydrogel degradation plays a crucial role in the release process of silver nanoparticles.

[0062] The silver nanoparticles released by the smart wound dressing can inhibit bacterial proliferation and have a broad-spectrum antibacterial effect. The most common bacteria that cause wound infection include Escherichia coli and Staphylococcus aureus. Escherichia coli and Staphylococcus aureus were used to characterize the antibacterial properties of the silver nanoparticles released by the hydrogel. Fig.18 As shown in the figure, the number of bacteria in the hydrogel group that was not incubated with matrix metalloproteinases remained almost unchanged, which was similar to the results of the negative control group, indicating that the hydrogel had a good encapsulation effect on the drug and had no antibacterial ability when not incubated with matrix metalloproteinases. In contrast, the number of bacteria in the hydrogel group that was incubated with matrix metalloproteinases decreased sharply, which was similar to the results of the positive control group, indicating that the silver nanoparticle drug released by the hydrogel had a significant antibacterial ability against Escherichia coli and Staphylococcus aureus.

[0063] The intelligent wound dressing of the present invention realizes in-situ detection of wound recovery and controlled-release antibacterial treatment. Wireless readout of chronic wound healing detection is realized through flexible inductor-capacitor resonant circuit and radio frequency technology. The use of bioresponsive hydrogel to deliver silver nanoparticles can inhibit bacterial proliferation in chronic wounds, and construct a highly sensitive and highly selective intelligent wound dressing for matrix metalloproteinases. Combined with a mobile terminal and a portable readout instrument, the intelligent wound dressing is easy to operate and use, and is not limited by the user and the place of use. The present invention can realize in-situ high-sensitivity wound matrix metalloproteinase detection and controlled release of antibacterial drugs, and can be effectively used for chronic wound management.

[0064] It should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Without making creative work, any modification, equivalent replacement, improvement, etc., shall be included in the protection scope of the present invention.

Claims

1. A smart wound dressing for matrix metalloproteinase detection and drug delivery, characterized in that: The smart wound dressing comprises, from top to bottom: A biosensitive hydrogel (1) comprising a bioactive arginyl-glycyl-aspartic acid polypeptide sequence for biorecognition of matrix metalloproteinases in wounds and controlled release of antimicrobial drugs according to the concentration of matrix metalloproteinases; Electrode packaging layer (2); Flexible interdigitated electrodes (3); Device packaging layer (4); A signal transmission antenna (5) connected to the flexible interdigital electrodes (3) for collecting and transmitting data; The skin adhesive layer (6) is used for adhesion to the skin.

2. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: The biosensitive hydrogel (1) is prepared by a photocuring method, specifically, adding 10 mg of methacrylated gelatin into 100 mL of PBS buffer containing 2 mg / mL of photoinitiator Irgacure 2959, stirring at 37° C. until completely dissolved, adding 100 mL of a silver nanoparticle dispersion having a concentration of 85 mg / mL, and mixing uniformly to obtain a first solution; taking 10 to 30 mL of the first solution and dripping it onto the surface of the interdigitated electrode, and irradiating with 8W, 365 nm ultraviolet light for cross-linking for 20 minutes.

3. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 2, characterized in that: The silver nanoparticle dispersion is prepared by an ascorbic acid / citric acid reduction method in the presence of anions, specifically, 2 mL of sodium citrate with a mass concentration of 1%, 0.5 mL of silver nitrate with a mass concentration of 1%, and 0.4 mL of 20 mM sodium chloride are sequentially added to 2.1 mL of water, and stirred for 5 minutes to obtain a second solution; the second solution is added to 94.9 mL of boiling water containing 100 mL of 0.1 M ascorbic acid solution, and boiling and stirring are continued for 1 hour to obtain a silver nanoparticle dispersion.

4. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: The electrode packaging layer (2) is made of epoxy resin material, prepared by spin coating and UV curing, has a thickness of 1 to 5 μm, and covers the interdigital part of the flexible interdigital electrode (3) to ensure that a frequency response signal is obtained at a lower frequency.

5. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: The device packaging layer (4) is made of polydimethylsiloxane material and has a thickness of 50 to 200 μm, and is used to package and protect the signal transmission antenna.

6. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: The inductance of the signal transmission antenna (5) is 0.2-0.5 μH.

7. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: The flexible interdigital electrodes (3) and the signal transmission antenna (5) are electrically connected by welding.

8. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: The skin adhesive layer (6) is made of polyurethane.

9. The smart wound dressing for matrix metalloproteinase detection and drug delivery according to claim 1, characterized in that: It also includes a wireless terminal coupled to the signal transmission antenna (5) for wirelessly reading out the chronic wound healing condition detection signal.

10. An application of the smart wound dressing according to any one of claims 1 to 9, characterized in that: The smart wound dressing is attached to a wound for wound matrix metalloproteinase detection and / or drug controlled release.

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