Method for confinement preparation of nanoparticles based on biological template method

Abstract

The invention provides a preparation method of nanoparticles, and belongs to the technical field of preparation of nanoparticles by a template method. According to the preparation method of the nanoparticles, the adopted main raw materials are periostracum cicada, zinc nitrate hexahydrate, cobalt nitrate hexahydrate, 2-methylimidazole and methanol, and the price is low. The synthesis process can be completed at room temperature, is simple to operate, does not have a high-temperature treatment link, and is relatively safe. The growth of the nanoparticles mainly depends on the confinement effect of the mold of the anti-structure holes in the surface of the cicada wing, and the growth of the nanoparticles is limited through the inherent size of the anti-structure holes in the surface of the cicada wing, so that the nanoparticles with specific sizes and shapes are prepared.

Description

Technical Field

[0001] The invention belongs to the technical field of preparing nanoparticles by template method, and specifically relates to a method for preparing nanoparticles by confinement of a biological template based on the surface structure of cicada wings. Background Art

[0002] Metal-Organic Framework (MOF) is a low-density crystalline porous material formed by the coordination of metal ions or metal clusters with organic ligands. Due to its large specific surface area, high porosity, controllable crystal structure and pore size, and rich alternative components, it is widely used in energy storage, gas storage, adsorption and separation of pollutants and other fields. It is also widely used in the field of biomedical materials due to its low toxicity, simple preparation method, and good biodegradability. Zeolitic imidazolate frameworks (ZIFs) are a subclass of MOFs, composed of transition metals (such as Fe, Co, Cu, Zn) coordinated with nitrogen atoms of imidazole ligands. Because ZIFs have the same structure as traditional zeolites, this material has the application prospects of traditional zeolite structures and the advantages of MOFs such as controllability, easy modification, and low toxicity.

[0003] ZIFs materials have more than 100 different topological structures, which can be synthesized by adjusting the central ion, organic ligand and reaction conditions (temperature, pressure, etc.). ZIF-8 is the most common porous material in ZIFs. 2+ ZIF-67 is another common porous material with a rhombic dodecahedron topology, which is composed of nitrogen atoms on the 2-methylimidazole ring and metal Co 2+ The coordination bonds are formed to form a four-coordinate framework with Co-imidazole ester. At present, there are various methods for preparing ZIF-8 or ZIF-67, mainly including: solvent thermal synthesis, hydrothermal method, ultrasonic synthesis method, etc., but these preparation methods are relatively complicated and it is difficult to accurately control the size of nanoparticles.

[0004] The cicada wing selected by the present invention is a black grasshopper cicada that is more common in nature, and a nanometer sub-dome cone array structure is evenly distributed on its surface, and its base circle diameter is about 180±10nm, the top circle diameter is about 90±10nm, the overall height is about 325±15nm, and the distance between adjacent structural units, that is, the structural period is about 200±10nm. The natural nanostructure on the surface of the cicada wing combined with the sol-gel method can obtain an anti-structure pore mold with corresponding size. Based on the anti-structure pore mold on the surface of the cicada wing, nanoparticles with certain regular sizes such as ZIF-8 and ZIF-67 can be confined to grow. This preparation method is superior to the previous traditional nanoparticle preparation method, and the advantage is that the anti-structure nanopore template on the surface of the cicada wing can be used for the confined preparation of nanoparticles with relatively uniform size. Summary of the invention

[0005] In order to overcome the problems existing in the prior art, the present invention provides a method for preparing nanoparticles based on biological template confinement, which is low-cost and simple to operate. The natural structure of the surface of cicada wings is used as the original biological template to prepare an anti-structure pore mold, and then the nanoparticles confined by the anti-structure pore mold have a relatively uniform size.

[0006] To achieve the above-mentioned purpose of the invention, the technical solution of the present invention is as follows:

[0007] A method for preparing nanoparticles by confinement based on a biological template method comprises the following steps:

[0008] (1) Preparation process of the mold for the reverse structure hole on the cicada wing surface:

[0009] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0010] (1.2) Pre-treating the anti-structure hole mold material, and then placing it in a vacuum drying oven for a period of time to allow the material to fully infiltrate the cicada wing;

[0011] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the reverse structure hole mold;

[0012] (2) Nanoparticle preparation process:

[0013] (2.1) dissolving zinc nitrate hexahydrate or cobalt nitrate in methanol and stirring evenly to obtain a zinc nitrate / cobalt nitrate solution;

[0014] (2.2) adding 2-methylimidazole to methanol and stirring to obtain a 2-methylimidazole solution, adding the solution to the zinc nitrate or cobalt nitrate solution to obtain a mixture solution, and mixing the mixture solution using an oscillator;

[0015] (2.3) Apply a certain amount of the mixed liquid to the inverse structure hole mold to ensure that the liquid fully immerses the mold, and let it stand for a period of time to react;

[0016] (2.4) Take out the inverse structure hole mold and repeatedly rinse the mold surface with methanol and deionized water;

[0017] (2.5) The rinsed mold is placed in a vacuum drying oven for vacuum drying for a period of time, and confined growth of nanoparticles is obtained in the inverse structure pores of the mold.

[0018] As a preferred embodiment, the mass of zinc nitrate hexahydrate or cobalt nitrate in step (2.1) is 0.1 g, and the volume of methanol is 8.5 ml; the mass of 2-methylimidazole in step (2.2) is 1.0 g, and the volume of methanol is 8.5 ml; 8.5 ml of 2-methylimidazole solution is added to 8.5 ml of the above zinc nitrate or cobalt nitrate solution.

[0019] As a preferred embodiment, the base circle diameter of the cicada wing is about 180±10nm.

[0020] As a preferred embodiment, the reverse structure hole mold material is ultraviolet light curing glue or polymethyl methacrylate PMMA.

[0021] As a preferred method, the reverse structure hole mold preparation process when the reverse structure hole mold material is ultraviolet light curing glue is:

[0022] (1.1): The original cicada wings were ultrasonically treated with acetone, ethanol and deionized water, and then dried naturally to obtain clean cicada wings for use;

[0023] (1.2) evenly coating the cicada wings with UV curing adhesive liquid, and then placing the cicada wings in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0024] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the ultraviolet light-cured glue reverse structure hole mold.

[0025] As a preferred method, the preparation process of the reverse structure hole mold when the reverse structure hole mold material is PMMA is as follows:

[0026] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0027] (1.2) Drying PMMA powder, then dissolving PMMA in chloroform and stirring thoroughly until the solution is colorless and transparent;

[0028] (1.3) Evenly coat the PMMA liquid on the cicada wings, and then place them in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0029] (1.4) After multiple coatings, the PMMA inverse structure hole mold is obtained by natural cooling and peeling.

[0030] As a preferred embodiment, the UV curing adhesive is NOA61.

[0031] As a preferred embodiment, the static reaction time in step (2.3) is 10-30 min.

[0032] The effective effects of the present invention are as follows: the present invention is a method for preparing nanoparticles by a biological template method, the main raw materials used are cicada wings, and PMMA and UV-curable adhesive reverse structure hole molds are prepared based on the natural structure of the surface of cicada wings, and zinc nitrate hexahydrate, cobalt nitrate hexahydrate, 2-methylimidazole, and methanol are cheap and easily available. The preparation process can be stirred and mixed at room temperature, the operation is simple, there is no high-temperature treatment process, and it is relatively safe. The growth of nanoparticles mainly depends on the confinement effect of the reverse structure holes on the surface of cicada wings, and the particles are prepared by the confinement effect of the inherent size of the biological template on its particle growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a diagram of ZIF-8 prepared based on NOA61 in the present invention;

[0034] It can be seen from the figure that the present invention confinedly grows nanoparticles based on the anti-structure pore template. DETAILED DESCRIPTION

[0035] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0036] A method for preparing nanoparticles by confinement based on a biological template method comprises the following steps:

[0037] (1) Preparation process of the mold for the reverse structure hole on the cicada wing surface:

[0038] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0039] (1.2) Pre-treating the anti-structure hole mold material, and then placing it in a vacuum drying oven for a period of time to allow the material to fully infiltrate the cicada wing;

[0040] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the reverse structure hole mold;

[0041] (2) Nanoparticle preparation process:

[0042] (2.1) dissolving zinc nitrate hexahydrate or cobalt nitrate in methanol and stirring evenly to obtain a zinc nitrate / cobalt nitrate solution;

[0043] (2.2) adding 2-methylimidazole to methanol and stirring to obtain a 2-methylimidazole solution, adding the solution to the zinc nitrate or cobalt nitrate solution to obtain a mixture solution, and mixing the mixture solution using an oscillator;

[0044] (2.3) Apply a certain amount of the mixed liquid to the inverse structure hole mold to ensure that the liquid fully immerses the mold, and let it stand for a period of time to react;

[0045] (2.4) Take out the inverse structure hole mold and repeatedly rinse the mold surface with methanol and deionized water;

[0046] (2.5) The rinsed mold is placed in a vacuum drying oven for vacuum drying for a period of time, and confined growth of nanoparticles is obtained in the inverse structure pores of the mold.

[0047] In some embodiments, the mass of zinc nitrate hexahydrate or cobalt nitrate in step (2.1) is 0.1 g, and the volume of methanol is 8.5 ml; the mass of 2-methylimidazole in step (2.2) is 1.0 g, and the volume of methanol is 8.5 ml; 8.5 ml of 2-methylimidazole solution is added to 8.5 ml of the above zinc nitrate or cobalt nitrate solution.

[0048] In some embodiments, the base circle diameter of the cicada wing is about 180±10 nm.

[0049] In some embodiments, the reverse structure hole mold material is UV curable adhesive or polymethyl methacrylate (PMMA).

[0050] In some embodiments, when the reverse structure hole mold material is UV curable adhesive, the reverse structure hole mold preparation process is as follows:

[0051] (1.1): The original cicada wings were ultrasonically treated with acetone, ethanol and deionized water, and then dried naturally to obtain clean cicada wings for use;

[0052] (1.2) evenly coating the cicada wings with UV curing adhesive liquid, and then placing the cicada wings in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0053] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the ultraviolet light-cured glue reverse structure hole mold.

[0054] In some embodiments, when the reverse structure hole mold material is PMMA, the reverse structure hole mold preparation process is as follows:

[0055] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0056] (1.2) Drying PMMA powder, then dissolving PMMA in chloroform and stirring thoroughly until the solution is colorless and transparent;

[0057] (1.3) Evenly coat the PMMA liquid on the cicada wings, and then place them in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0058] (1.4) After multiple coatings, the PMMA inverse structure hole mold is obtained by natural cooling and peeling.

[0059] In some embodiments, the UV curable adhesive is NOA61.

[0060] In some embodiments, the static reaction time in step (2.3) is 10-30 min.

[0061] Example 1: Preparation of ZIF-8 based on UV-curable adhesive reverse structure hole mold

[0062] (1) Preparation process of the mold for the reverse structure hole on the cicada wing surface:

[0063] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0064] (1.2) evenly coating the cicada wings with UV curing adhesive liquid, and then placing the cicada wings in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0065] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to completely cure the liquid glue, and peel them off to obtain the ultraviolet light-cured glue reverse structure hole mold;

[0066] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the reverse structure hole mold;

[0067] (2) Nanoparticle preparation process:

[0068] (2.1) Weigh 0.1 g of zinc nitrate hexahydrate using an analytical balance, add it to 8.5 ml of methanol, and stir well to obtain a zinc nitrate solution.

[0069] (2.2) Weigh 1.0 g of 2-methylimidazole using an analytical balance, add it to 8.5 ml of methanol, stir evenly, and pour it into the zinc nitrate solution obtained in step 1 to obtain a mixed solution.

[0070] (2.3) The mixture solution was mixed evenly with an oscillator, and a certain amount of the mixture was applied to the reverse structure hole mold prepared with UV curing adhesive NOA61 to ensure that the liquid could fully immerse the mold, and then allowed to stand for 10 minutes.

[0071] (2.4) Take out the anti-structure hole mold and repeatedly rinse the mold surface with methanol and deionized water.

[0072] (2.5) The rinsed mold is placed in a drying oven to dry for a period of time, and confined growth of nanoparticles ZIF-8 is obtained in the inverse structure pores of the mold.

[0073] The cicada wing selected by the present invention is a black grasshopper cicada that is more common in nature, and a nanometer sub-dome cone array structure is evenly distributed on its surface, and its base circle diameter is about 180±10nm, the top circle diameter is about 90±10nm, the overall height is about 325±15nm, and the distance between adjacent structural units, that is, the structural period is about 200±10nm. The natural nanostructure on the surface of the cicada wing combined with the sol-gel method can obtain an anti-structure pore mold with corresponding size. Based on the anti-structure pore mold on the surface of the cicada wing, nanoparticles with certain regular sizes such as ZIF-8 and ZIF-67 can be confined to grow. This preparation method is superior to the previous traditional nanoparticle preparation method, and the advantage is that the anti-structure nanopore template on the surface of the cicada wing can be used for the confined preparation of nanoparticles with relatively uniform size.

[0074] Example 2: Preparation of ZIF-8 based on PMMA reverse structure pore mold

[0075] (1) Preparation process of the mold for the reverse structure hole on the cicada wing surface:

[0076] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0077] (1.2) Drying PMMA powder, then dissolving PMMA in chloroform and stirring thoroughly until the solution is colorless and transparent;

[0078] (1.3) Evenly coat the PMMA liquid on the cicada wings, and then place them in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0079] (1.4) After multiple coatings, the PMMA inverse structure hole mold is obtained by natural cooling and peeling.

[0080] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the reverse structure hole mold;

[0081] (2) Nanoparticle preparation process:

[0082] (2.1) Weigh 0.1 g of zinc nitrate hexahydrate using an analytical balance, add it to 8.5 ml of methanol, and stir well to obtain a zinc nitrate solution.

[0083] (2.2) Weigh 1.0 g of 2-methylimidazole using an analytical balance, add it to 8.5 ml of methanol, stir evenly, and pour it into the zinc nitrate solution obtained in step 1 to obtain a mixed solution.

[0084] (2.3) The mixture solution was mixed evenly with an oscillator, and a certain amount of the mixture was applied to the PMMA mold with inverse structure holes to ensure that the liquid could fully immerse the mold, and the reaction was continued for 20 minutes.

[0085] (2.4) Take out the anti-structure hole mold and repeatedly rinse the mold surface with methanol and deionized water.

[0086] (2.5) The rinsed mold is placed in a drying oven to dry for a period of time, and confined growth of nanoparticles ZIF-8 is obtained in the inverse structure pores of the mold.

[0087] The above two examples are both based on the confined growth of the same nanoparticle ZIF-8 based on different anti-structure hole molds.

[0088] The cicada wing selected by the present invention is a black grasshopper cicada that is more common in nature, and a nanometer sub-dome cone array structure is evenly distributed on its surface, and its base circle diameter is about 180±10nm, the top circle diameter is about 90±10nm, the overall height is about 325±15nm, and the distance between adjacent structural units, that is, the structural period is about 200±10nm. The natural nanostructure on the surface of the cicada wing combined with the sol-gel method can obtain an anti-structure pore mold with corresponding size. Based on the anti-structure pore mold on the surface of the cicada wing, nanoparticles with certain regular sizes such as ZIF-8 and ZIF-67 can be confined to grow. This preparation method is superior to the previous traditional nanoparticle preparation method, and the advantage is that the anti-structure nanopore template on the surface of the cicada wing can be used for the confined preparation of nanoparticles with relatively uniform size.

[0089] Example 3: Preparation of ZIF-67 based on UV-curable adhesive reverse structure hole mold

[0090] (1) Preparation process of the mold for the reverse structure hole on the cicada wing surface:

[0091] (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use;

[0092] (1.2) evenly coating the cicada wings with UV curing adhesive liquid, and then placing the cicada wings in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings;

[0093] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the ultraviolet light-cured glue reverse structure hole mold.

[0094] (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the reverse structure hole mold;

[0095] (2) Nanoparticle preparation process:

[0096] (2.1) Weigh 0.1 g of cobalt nitrate hexahydrate using an analytical balance, add it to 8.5 ml of methanol, and stir well to obtain a zinc nitrate solution.

[0097] (2.2) Weigh 1.0 g of 2-methylimidazole using an analytical balance, add it to 8.5 ml of methanol, stir evenly, and pour it into the cobalt nitrate solution obtained in step 1 to obtain a mixed solution.

[0098] (2.3) The mixture solution was mixed evenly with an oscillator, and a certain amount of the mixture was applied to the reverse structure hole mold prepared with UV curing adhesive NOA61 to ensure that the liquid could fully immerse the mold, and react for 30 minutes.

[0099] (2.4) Take out the anti-structure pore mold bio-template and repeatedly rinse the mold surface with methanol and deionized water.

[0100] (2.5) The rinsed mold is placed in a drying oven to dry for a period of time, and confined growth of nanoparticles ZIF-67 is obtained in the inverse structure pores of the mold.

[0101] The cicada wing selected by the present invention is a black grasshopper cicada that is more common in nature, and a nanometer sub-dome cone array structure is evenly distributed on its surface, and its base circle diameter is about 180±10nm, the top circle diameter is about 90±10nm, the overall height is about 325±15nm, and the distance between adjacent structural units, that is, the structural period is about 200±10nm. The natural nanostructure on the surface of the cicada wing combined with the sol-gel method can obtain an anti-structure pore mold with corresponding size. Based on the anti-structure pore mold on the surface of the cicada wing, nanoparticles with certain regular sizes such as ZIF-8 and ZIF-67 can be confined to grow. This preparation method is superior to the previous traditional nanoparticle preparation method, and the advantage is that the anti-structure nanopore template on the surface of the cicada wing can be used for the confined preparation of nanoparticles with relatively uniform size.

[0102] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical ideas disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing nanoparticles by confinement based on a biological template method, characterized in that: The following steps are involved: (1) Preparation process of the mold for the reverse structure hole on the cicada wing surface: (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use; (1.2) Pre-treating the anti-structure hole mold material, and then placing it in a vacuum drying oven for a period of time to allow the material to fully infiltrate the cicada wing; (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the reverse structure hole mold; (2) Nanoparticle preparation process: (2.1) dissolving zinc nitrate hexahydrate or cobalt nitrate in methanol and stirring evenly to obtain a zinc nitrate / cobalt nitrate solution; (2.2) adding 2-methylimidazole to methanol and stirring to obtain a 2-methylimidazole solution, adding the solution to the zinc nitrate or cobalt nitrate solution to obtain a mixture solution, and mixing the mixture solution using an oscillator; (2.3) Apply a certain amount of the mixed liquid to the inverse structure hole mold to ensure that the liquid fully immerses the mold, and let it stand for a period of time to react; (2.4) Take out the inverse structure hole mold and repeatedly rinse the mold surface with methanol and deionized water; (2.5) The rinsed mold is placed in a vacuum drying oven for vacuum drying for a period of time, and confined growth of nanoparticles is obtained in the inverse structure pores of the mold.

2. The method for preparing nanoparticles by confinement based on the biological template method according to claim 1, characterized in that: The mass of zinc nitrate hexahydrate or cobalt nitrate in step (2.1) is 0.1 g, and the volume of methanol is 8.5 ml; In step (2.2), the mass of 2-methylimidazole is 1.0 g, and the volume of methanol is 8.5 ml; 8.5 ml of the 2-methylimidazole solution is added to 8.5 ml of the above zinc nitrate or cobalt nitrate solution.

3. The method for preparing nanoparticles by confinement based on the biological template method according to claim 1, characterized in that: The base circle diameter of cicada wings is about 180±10nm.

4. The method for preparing nanoparticles by confinement based on the biological template method according to claim 1, characterized in that: The material of the reverse structure hole mold is ultraviolet curing glue or polymethyl methacrylate PMMA.

5. The method for preparing nanoparticles by confinement based on the biological template method according to claim 4, characterized in that: The preparation process of the reverse structure hole mold when the reverse structure hole mold material is UV curing glue is as follows: (1.1): The original cicada wings were ultrasonically treated with acetone, ethanol and deionized water, and then dried naturally to obtain clean cicada wings for use; (1.2) evenly coating the cicada wings with UV curing adhesive liquid, and then placing the cicada wings in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings; (1.3) Take out the fully soaked cicada wings, place them under ultraviolet light for a period of time to allow the liquid glue to completely solidify, and peel them off to obtain the ultraviolet light-cured glue reverse structure hole mold.

6. The method for preparing nanoparticles by confinement based on the biological template method according to claim 4, characterized in that: The preparation process of the anti-structure hole mold when the anti-structure hole mold material is PMMA is as follows: (1.1) treating the original cicada wings with acetone, ethanol and deionized water by ultrasonic treatment, and then drying them naturally to obtain clean cicada wings for use; (1.2) Drying PMMA powder, then dissolving PMMA in chloroform and stirring thoroughly until the solution is colorless and transparent; (1.3) Evenly coat the PMMA liquid on the cicada wings, and then place them in a vacuum drying oven for a period of time to allow the liquid to fully soak the cicada wings; (1.4) After multiple coatings, the PMMA inverse structure hole mold is obtained by natural cooling and peeling.

7. The method for preparing nanoparticles by confinement based on the biological template method according to claim 4, characterized in that: The UV curing adhesive is NOA61.

8. The method for preparing nanoparticles by confinement based on the biological template method according to claim 1, characterized in that: The standing reaction time in step (2.3) is 10-30 min.

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