Dual-fermentation thick skin factor liposome, preparation method thereof and cosmetics

Abstract

The invention provides a double-fermentation thick skin factor liposome, a preparation method thereof and a cosmetic, and relates to the technical field of biomedical materials. The double-fermentation thick skin factor liposome comprises alkaloid, a double-fermentation thick skin factor, phospholipid and short-chain fatty alcohol, the dual-fermentation skinning factor comprises galactose yeast fermentation liquor, lactic acid bacteria NP and glycyrrhetinic acid. According to the double-fermentation thick-skin factor liposome provided by the invention, the double-fermentation thick-skin factor has excellent anti-aging and anti-aging effects, can remarkably improve the cell activity and enhance the skin barrier, and is prepared into a liposome form, and glycyrrhetinic acid in the double-fermentation thick-skin factor and sophocarpidine are utilized to form a supramolecular eutectic. The double-fermentation thick skin factor liposome has double advantages of the supramolecular eutectic solvent and the liposome, the double-fermentation thick skin factor is efficiently promoted to enter the skin, the skin residence rate is increased, and the local treatment effect is effectively improved to play the anti-aging effect of the double-fermentation thick skin factor liposome.

Description

Technical Field

[0001] The invention relates to the technical field of biomedical materials, in particular to a double-fermented pachydermogen liposome and a preparation method and cosmetics thereof. Background Art

[0002] Double fermented thick skin factor (named double fermented thick skin factor LAB POR) mainly contains galactosyl yeast fermentation broth, lactic acid bacteria NP, and glycyrrhizic acid. Lactobacillus NP is a product obtained from the lysate produced by the fermentation of Lactococcus lactis. It contains all the active ingredients of the bacteria itself and the fermentation process, and has a good anti-aging effect. Lactic acid bacteria fermentation technology can convert large molecular functional substances into small molecules, maximize the retention of active substances, and promote the direct absorption of nutrients by the skin. Lactic acid bacteria lysate can improve the quality and diversity of skin microorganisms. At the same time, the polypeptide substances such as lactobacillus contained in it can adhere to the skin surface and act as an antibacterial barrier. The amino acids, polysaccharides, B vitamins, etc. in the lactic acid bacteria lysate can enhance the metabolism of the stratum corneum, remove free radicals, and activate the body's repair properties. The lactic acid and sodium lactate contained in the lactic acid bacteria lysate can form an acid mask, maintaining the skin at a healthy weakly acidic pH to improve the sub-healthy state of the skin. In addition, the lactic acid bacteria fermentation lysate can promote the synthesis of collagen and hyaluronic acid, and has a long-lasting and deep moisturizing effect on the skin.

[0003] Galactose yeast fermentation liquid is rich in bio-oligosaccharides, amino acids, organic acids, vitamins and other ingredients, which can improve skin barrier function and cell vitality, making the skin more delicate and plump. Long-term use has significant improvement effects.

[0004] Liposomes are a new type of drug carrier, which is composed of phospholipids and other amphiphilic substances dispersed in water to form one or more concentric liposome bimolecular membranes encapsulating ultra-microscopic spherical drug carriers. Liposomes can encapsulate water-soluble or fat-soluble drugs. Their structure is similar to biological membranes, so they can enhance the degree of entry of active ingredients into the stratum corneum or epidermal lipids, increase the accumulation of active ingredients in the local skin, improve bioavailability, and have a sustained release effect. Glycyrrhetinic acid is a pentacyclic triterpenoid saponin extracted from the plant licorice, which has anti-inflammatory, anti-allergic and antibacterial effects. Glycyrrhetinic acid has an amphiphilic structural characteristic similar to that of surfactants. It can interact with cholesterol or phospholipids on the cell membrane, change the fluidity of lipids, increase the permeability of the cell membrane and the ability of drugs to cross the membrane. The hydrophilicity of glycyrrhetinic acid comes from the two glucuronic acids and one carboxyl group in its structure. It has poor water solubility and is greatly affected by pH, which limits its scope of application.

[0005] Supramolecular eutectic solvents are transparent solutions formed by mixing two or more different hydrogen bond acceptors and hydrogen bond donors at a certain molar ratio at a certain temperature. Their properties are similar to those of ionic liquids. Eutectic treatment can reduce the irritation of raw materials, improve solubility and stability, and enhance functionality and permeability.

[0006] However, the double-fermented thick skin factor LAB POR has the disadvantages of easy sedimentation and low transdermal efficiency, which limits its application.

[0007] In view of this, the present invention is proposed. Summary of the invention

[0008] One of the purposes of the present invention is to provide a double fermented pachyme liposome to solve at least one of the above technical problems in the prior art.

[0009] The second object of the present invention is to provide a method for preparing double-fermented pachyme liposomes.

[0010] A third object of the present invention is to provide a cosmetic.

[0011] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0012] The first aspect of the present invention provides a double fermented pachydermogen liposome, comprising alkaloids, double fermented pachydermogen, phospholipids and short chain fatty alcohols;

[0013] The double fermentation thick skin factor comprises galactosyl yeast fermentation liquid, lactic acid bacteria NP and glycyrrhizic acid.

[0014] Furthermore, in the double-fermented thick skin factor, the mass ratio of galactosyl yeast fermentation broth, lactic acid bacteria NP and glycyrrhizic acid is (5-15):(40-60):1.

[0015] Furthermore, the preparation method of the double fermentation thick skin factor is:

[0016] The galactose yeast fermentation liquid, lactic acid bacteria NP and glycyrrhetinic acid are uniformly mixed and then homogenized for 1 to 3 times to obtain the double fermentation thick skin factor.

[0017] Wherein, the homogenization pressure is 8000-10000psi.

[0018] Furthermore, the double fermented pachydermogen liposome comprises, by mass percentage, 0.2-2% of alkaloids, 6-55% of double fermented pachydermogen, 3-8% of phospholipids and 39-89% of short chain fatty alcohols.

[0019] Furthermore, the alkaloid comprises at least one of choline chloride, betaine and matrine, preferably matrine.

[0020] Preferably, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propylene glycol, 1,3-propylene glycol, glycerol, butylene glycol, pentanediol and hexanediol, preferably butylene glycol or glycerol.

[0021] Furthermore, the phospholipids include soybean lecithin and / or hydrogenated lecithin, preferably soybean lecithin.

[0022] The second aspect of the present invention provides a method for preparing the double-fermented pachyme liposomes, comprising the following steps:

[0023] A. Evenly mixing the alkaloid and the double fermented pachyderm factor to obtain the double fermented pachyderm factor modified by the supramolecular eutectic solvent;

[0024] B. dissolving phospholipids in short-chain fatty alcohols to obtain a phospholipid solution;

[0025] C. adding the phospholipid solution to the double fermented pachyderm factor modified by the supramolecular eutectic solvent and mixing evenly to obtain a sample solution;

[0026] D. homogenizing the sample solution to obtain double-fermented pachyme liposomes.

[0027] Furthermore, in step A, the mixing temperature is 50-70° C. and the mixing time is 15-30 min.

[0028] Preferably, in step B, the dissolving temperature is 70-90°C.

[0029] Furthermore, in step C, the temperature of the phospholipid solution is 45-55°C.

[0030] Preferably, in step C, the mixing speed is 300-500 rpm and the mixing time is 10-15 min.

[0031] Preferably, in step D, the homogenization pressure is 15000-25000 psi, the number of cycles is 1-5 times, and the discharge temperature is 10-30°C.

[0032] The third aspect of the present invention provides a cosmetic comprising the double-fermented pachydermogen liposomes described in the first aspect.

[0033] Compared with the prior art, the present invention has at least the following beneficial effects:

[0034] The double fermented pachyderm factor liposome provided by the present invention has excellent anti-aging and anti-aging effects, can significantly improve cell vitality, and enhance skin barrier. It is made into a liposome form, and glycyrrhetinic acid and matrine in the double fermented pachyderm factor are used to form a supramolecular eutectic, which not only helps the transdermal absorption of the double fermented pachyderm factor, but also can reduce the irritation of the double fermented pachyderm factor to make it milder. The double fermented pachyderm factor liposome has the dual advantages of a supramolecular eutectic solvent and a liposome, efficiently promotes the double fermented pachyderm factor to enter the skin, improves the skin retention rate, and effectively improves the local treatment effect to exert its anti-aging and anti-aging effects.

[0035] The preparation method of the double fermented pachyderm factor liposome provided by the present invention significantly improves the performance of the double fermented pachyderm factor, including its stability, biocompatibility, transdermal absorption efficiency and skin care effect, through supramolecular eutectic solvent modification and phospholipid encapsulation technology. The preparation method provides a solid technical foundation for the application of the double fermented pachyderm factor in high-end cosmetics and skin care products.

[0036] The cosmetics provided by the present invention, in view of the advantages of the above-mentioned double-fermented thick skin factor liposomes, enable the prepared cosmetics to enhance the anti-aging and anti-aging effects, and also improve the safety, stability and transdermal absorption capacity of the product, thereby providing consumers with an efficient, safe and widely applicable anti-aging option. DETAILED DESCRIPTION

[0037] The embodiments of the present invention will be described in detail below in conjunction with the embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only used to illustrate the present invention and should not be considered to limit the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0038] The first aspect of the present invention provides a double fermented pachydermogen liposome, comprising alkaloids, double fermented pachydermogen, phospholipids and short chain fatty alcohols;

[0039] The double fermentation thick skin factor comprises galactosyl yeast fermentation liquid, lactic acid bacteria NP and glycyrrhizic acid.

[0040] The double fermented pachyderm factor liposome provided by the present invention has excellent anti-aging and anti-aging effects, can significantly improve cell vitality, and enhance skin barrier. It is made into a liposome form, and glycyrrhetinic acid and matrine in the double fermented pachyderm factor are used to form a supramolecular eutectic, which not only helps the transdermal absorption of the double fermented pachyderm factor, but also can reduce the irritation of the double fermented pachyderm factor to make it milder. The double fermented pachyderm factor liposome has the dual advantages of a supramolecular eutectic solvent and a liposome, efficiently promotes the double fermented pachyderm factor to enter the skin, improves the skin retention rate, and effectively improves the local treatment effect to exert its anti-aging and anti-aging effects.

[0041] In the double fermented thick skin factor, glycyrrhetinic acid can participate in the formation of supramolecular eutectics, which can increase the stability of the active substance and reduce the pH to make the active ingredient more mild; in addition, glycyrrhetinic acid is amphiphilic and can be embedded in the arranged phosphatidylcholine to participate in the construction of liposomes, which enables the liposomes to remain stable for a long time in the temperature range of -20°C to 50°C. In the present invention, the supramolecular eutectics formed by glycyrrhetinic acid are embedded or partially embedded in the liposome membrane, which can cooperate with the liposome to exert the penetration ability, more efficiently promote the double fermented thick skin factor LAB POR to enter the skin, improve the skin residence rate, and effectively improve the local treatment effect to exert its anti-aging and anti-aging effects. The in vitro transdermal test guidelines specified by the OECD / OCDE "European OECD Chemical Testing Guidelines" were used for verification, and glycyrrhetinic acid was used as the target for testing. After the supramolecular co-crystal liposome was diluted, its skin retention was increased by about 6 times compared with the glycyrrhetinic acid solution of the same concentration prepared using a solubilizer. At the same time, glycyrrhetinic acid was not detected in the receiving pool, which indicates that while the supramolecular co-crystal liposome increased the skin retention rate, it did not penetrate the dermis and enter the blood circulation.

[0042] Lactic acid bacteria NP is mainly composed of 99.17% lactic acid bacteria fermentation lysate and a small amount of glycerol and other ingredients. Its English name is ProRenew Complex CLR NP, and its Chinese abbreviation is Lactic acid bacteria NP.

[0043] Furthermore, in the double-fermented thick skin factor, the mass ratio of galactosyl yeast fermentation broth, lactic acid bacteria NP and glycyrrhizic acid is (5-15):(40-60):1.

[0044] Typically but not restrictively, the mass ratio of galactosidase fermentation broth, lactic acid bacteria NP and glycyrrhizic acid can be the following specific combinations: 5:40:1, 5:50:1, 5:60:1, 10:40:1, 10:50:1, 10:60:1, 15:40:1, 15:50:1 or 15:60:1, or it can be any value within the range of (5-15):(40-60):1.

[0045] Furthermore, the preparation method of the double fermentation thick skin factor is:

[0046] The galactose yeast fermentation liquid, lactic acid bacteria NP and glycyrrhetinic acid are uniformly mixed and then homogenized for 1 to 3 times to obtain the double fermentation thick skin factor.

[0047] Wherein, the homogenization pressure is 8000-10000psi.

[0048] Typically but not limiting, the homogenization pressure may be, for example, 8000 psi, 8500 psi, 9000 psi, 9500 psi, 10000 psi, or any value within the range of 8000 psi to 10000 psi.

[0049] Furthermore, the double fermented pachydermogen liposome comprises, by mass percentage, 0.2-2% of alkaloids, 6-55% of double fermented pachydermogen, 3-8% of phospholipids and 39-89% of short chain fatty alcohols.

[0050] Typically but not limiting, in the mass percentage of the double fermented pachyderm factor liposomes, the proportion of alkaloids can be 0.2%, 0.5%, 1%, 1.5%, 2%, or any value within the range of 0.2% to 2%; the proportion of double fermented pachyderm factor can be 6%, 10%, 20%, 30%, 40%, 50%, 55%, or any value within the range of 6% to 55%; the proportion of phospholipids can be 3%, 4%, 5%, 6%, 7%, 8%, or any value within the range of 3% to 8%; the proportion of short-chain fatty alcohols can be 39%, 45%, 55%, 65%, 75%, 85%, 89%, or any value within the range of 39% to 89%.

[0051] Furthermore, the alkaloid comprises at least one of choline chloride, betaine and matrine, preferably matrine.

[0052] Preferably, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propylene glycol, 1,3-propylene glycol, glycerol, butylene glycol, pentanediol and hexanediol, preferably butylene glycol or glycerol.

[0053] Furthermore, the phospholipids include soybean lecithin and / or hydrogenated lecithin, preferably soybean lecithin.

[0054] The second aspect of the present invention provides a method for preparing the double-fermented pachyme liposomes, comprising the following steps:

[0055] A. Evenly mixing the alkaloid and the double fermented pachyderm factor to obtain the double fermented pachyderm factor modified by the supramolecular eutectic solvent;

[0056] B. dissolving phospholipids in short-chain fatty alcohols to obtain a phospholipid solution;

[0057] C. adding the phospholipid solution to the double fermented pachyderm factor modified by the supramolecular eutectic solvent and mixing evenly to obtain a sample solution;

[0058] D. homogenizing the sample solution to obtain double-fermented pachyme liposomes.

[0059] The preparation method of the double fermented pachyderm factor liposome provided by the present invention significantly improves the performance of the double fermented pachyderm factor, including its stability, biocompatibility, transdermal absorption efficiency and skin care effect, through supramolecular eutectic solvent modification and phospholipid encapsulation technology. The preparation method provides a solid technical foundation for the application of the double fermented pachyderm factor in high-end cosmetics and skin care products.

[0060] Furthermore, in step A, the mixing temperature is 50-70° C. and the mixing time is 15-30 min.

[0061] Typically but not limiting, the mixing temperature in step A can be, for example, 50°C, 55°C, 60°C, 65°C, 70°C, or any value within the range of 50°C to 70°C; the mixing time can be, for example, 15 min, 20 min, 25 min, 30 min, or any value within the range of 15 min to 30 min.

[0062] Preferably, in step B, the dissolving temperature is 70-90°C.

[0063] Typically but not limiting, the dissolving temperature in step B can be, for example, 70°C, 75°C, 80°C, 85°C, 90°C, or any value within the range of 70°C to 90°C.

[0064] Furthermore, in step C, the temperature of the phospholipid solution is 45-55°C.

[0065] Typically but not limiting, the temperature of the phospholipid solution in step C can be, for example, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C or 90°C, or any value within the range of 45°C to 90°C.

[0066] Preferably, in step C, the mixing speed is 300-500 rpm and the mixing time is 10-15 min.

[0067] Typically but not limiting, the mixing speed in step C can be, for example, 300 rpm, 350 rpm, 400 rpm, 450 rpm, 500 rpm, or any value in the range of 300 rpm to 500 rpm; the mixing time can be, for example, 10 min, 12 min, 14 min, or any value in the range of 10 min to 15 min.

[0068] Preferably, in step D, the homogenization pressure is 15000-25000 psi, the number of cycles is 1-5 times, and the discharge temperature is 10-30°C.

[0069] Typically but not limiting, the homogenization pressure in step D can be, for example, 15000psi, 18000psi, 21000psi, 24000psi, or any value in the range of 15000psi to 25000psi; the number of cycles can be, for example, 1 time, 2 times, 3 times, 4 times, or any value in the range of 1 time to 5 times; the discharge temperature can be, for example, 10°C, 15°C, 20°C, 25°C, 30°C, or any value in the range of 10°C to 30°C.

[0070] The third aspect of the present invention provides a cosmetic comprising the double-fermented pachydermogen liposomes described in the first aspect.

[0071] The cosmetics provided by the present invention, in view of the advantages of the above-mentioned double-fermented thick skin factor liposomes, enable the prepared cosmetics to enhance the anti-aging and anti-aging effects, and also improve the safety, stability and transdermal absorption capacity of the product, thereby providing consumers with an efficient, safe and widely applicable anti-aging option.

[0072] The present invention is further described below by specific examples and comparative examples, but it should be understood that these examples are only used for more detailed description and should not be understood as limiting the present invention in any form. The raw materials used in the examples and comparative examples of the present invention, if no specific conditions are specified, are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used without indicating the manufacturer are all conventional products that can be purchased commercially.

[0073] Examples 1-7

[0074] These embodiments provide a double fermented pachyme liposome, and the dosage of the raw materials is shown in the following Table 1.

[0075] Table 1

[0076]

[0077] The preparation method is as follows:

[0078] 1. Glycyrrhetinic acid, lactic acid bacteria NP and galactosyl yeast fermentation broth were mixed evenly, and homogenized twice at 9000psi to obtain double fermentation thick skin factor LAB POR.

[0079] 2. Stir the double fermented pachyderm factor LAB POR and matrine at 60°C for 20 minutes to obtain the double fermented pachyderm factor LAB POR modified with a supramolecular eutectic solvent.

[0080] 3. Mix soybean lecithin and butylene glycol, then heat to 80°C and stir until the soybean lecithin is completely dissolved, then cool to 50°C to obtain a soybean lecithin solution.

[0081] 4. Add the soybean lecithin solution into the double fermented thick skin factor LAB POR modified by the supramolecular eutectic solvent, set the rotation speed to 400 rpm, the time to 15 min, and obtain the sample solution.

[0082] 5. The sample liquid was homogenized by a homogenizer, the homogenization pressure was controlled to be 20000psi, the number of cycles was 3 times, and the discharge temperature was 25°C, and the double fermentation thick skin factor LAB POR liposomes were obtained.

[0083] Comparative Example 1

[0084] This comparative example provides a double fermented thick skin factor LAB POR emulsion, which is composed of the following components:

[0085] Double fermented thick skin factor LAB POR is 30.5%, PPG-13-decyltetradeceth-24 is 5% and butanediol is 64.5%;

[0086] The double fermented pachyderm factor LAB POR contains 0.5% glycyrrhetinic acid, 25% lactic acid bacteria NP and 5% galactosyl yeast fermentation liquid. The preparation method of the double fermented pachyderm factor LAB POR is the same as that in the embodiment.

[0087] The preparation method of double fermented thick skin factor LAB POR emulsion is as follows:

[0088] The double fermented thick skin factor LAB POR and PPG-13-decyltetradecyl alcohol polyether-24 are mixed and stirred until transparent; then butanediol is added and mixed evenly, and high-pressure homogenization is performed at a homogenization pressure of 15000psi, and homogenization is performed 3 to 5 times to obtain a double fermented thick skin factor LAB POR emulsion.

[0089] Comparative Example 2

[0090] This comparative example provides a supramolecular eutectic double fermented thick skin factor LAB POR emulsion, which is composed of the following components:

[0091] The double fermentation pachyme factor LAB POR was 30.5%, matrine 0.6% and butanediol 68.9%;

[0092] The double fermented pachyderm factor LAB POR contains 0.5% glycyrrhetinic acid, 25% lactic acid bacteria NP and 5% galactosyl yeast fermentation liquid. The preparation method of the double fermented pachyderm factor LAB POR is the same as that in the embodiment.

[0093] The preparation method of supramolecular eutectic double fermentation thick skin factor LAB POR emulsion is as follows:

[0094] The double fermented pachyderm factor LAB POR and matrine were mixed and stirred at 60°C for 20 minutes to obtain a double fermented pachyderm factor LAB POR modified with a supramolecular eutectic solvent; then butanediol was added thereto and mixed evenly, and high-pressure homogenization was performed at a homogenization pressure of 15,000 psi, and homogenization was performed 3 to 5 times to obtain a supramolecular eutectic double fermented pachyderm factor LAB POR emulsion.

[0095] Comparative Example 3

[0096] This comparative example provides a double fermented pachyderm factor LAB POR liposome, which is composed of the following components:

[0097] Double fermented thick skin factor LAB POR is 30.5%, PPG-13-decyltetradecyl polyether-24 is 5%, soy lecithin is 5%, butylene glycol is 15% and glycerol is 44.5%;

[0098] The double fermented pachyderm factor LAB POR contains 0.5% glycyrrhetinic acid, 25% lactic acid bacteria NP and 5% galactosyl yeast fermentation liquid. The preparation method of the double fermented pachyderm factor LAB POR is the same as that in the embodiment.

[0099] The preparation method of double fermented pachyderm factor LAB POR liposomes is as follows:

[0100] First, PPG-13-decyltetradecanol polyether-24, soybean lecithin and butylene glycol are stirred at 80°C until uniformly dispersed, and then the temperature is lowered to 50°C to obtain a mixed solution; then, the double fermented thick skin factor LAB POR and glycerol are evenly mixed and added to the mixed solution for high-pressure homogenization at a homogenization pressure of 15000psi for 3 to 5 times to obtain the double fermented thick skin factor LABPOR liposomes.

[0101] Test Example 1

[0102] The liposomes or emulsions obtained in the examples and comparative examples were subjected to dynamic light scattering detection, and their average particle sizes after standing for 24 hours were measured, and their appearances after 30 days were observed. The results are recorded in Table 1.

[0103] Table 1

[0104]

[0105]

[0106] Test Example 2

[0107] The liposomes or emulsions obtained in the examples and comparative examples were compared in terms of penetration ability. The specific method is as follows:

[0108] Using glycyrrhetinic acid as a standard, weigh 50 g of liposomes or emulsions, then add 0.8 g of %SIMULGEL FL and 49.2 g of water in sequence and stir evenly at 50°C to obtain a test sample solution.

[0109] Skin retention rate test: The skin permeation test is carried out according to the in vitro permeation test guidelines stipulated by the OECD / OCDE European OECD Chemical Testing Guidelines.

[0110] The retention data are recorded in Table 2.

[0111] Table 2

[0112]

[0113] Comparative analysis of Table 1 and Table 2 shows that glycyrrhetinic acid is used as the active ingredient calibration component to explore the effect of changes in the content of the double fermented thick skin factor LAB POR on the skin retention and stability. According to the results of Example 1, Example 4, and Example 6, as the content of the double fermented thick skin factor LAB POR decreases, its stability does not change significantly. Example 6 appears slightly turbid because the decrease in the content of glycyrrhetinic acid causes the pH of the system to change, which in turn affects the stability of the liposomes; the skin retention of glycyrrhetinic acid decreases because as the content of glycyrrhetinic acid decreases, the number of eutectic molecules formed decreases, and the penetration-enhancing effect becomes worse. In Examples 1, 5 and 7, as the content of the double fermented thick skin factor LAB POR gradually increased, its stability decreased. This is because the increase in active ingredients caused the generation of two unstable factors. First, as the glycyrrhetinic acid increased, matrine was not sufficient to combine with all the glycyrrhetinic acid, and part of the glycyrrhetinic acid existed in a free state and then crystallization occurred; second, excessive double fermented thick skin factor LAB POR resulted in the inability of liposomes to completely encapsulate it, and demulsification occurred over time, and the skin retention amount of glycyrrhetinic acid also decreased accordingly.

[0114] Through the analysis of the skin retention amount of the comparative examples and the embodiments, it can be seen that both the co-crystal solvent and the liposome can promote the transdermal ability of glycyrrhetinic acid; the embodiment can significantly enhance the ability of glycyrrhetinic acid to penetrate the skin by embedding the co-crystal into the liposome, so the combination of the co-crystal and the liposome can exert a synergistic penetration-enhancing effect.

[0115] The glycyrrhetinic acid contained in the double fermented thick skin factor LAB POR can form a supramolecular eutectic with matrine, which can cooperate with the liposomes formed by it to promote the transdermal absorption of active ingredients. According to Examples 1-3, it can be known that only when glycyrrhetinic acid and matrine are combined in an appropriate ratio can they have a good penetration-promoting effect and maintain high stability at the same time. The low content of matrine has little effect on the particle size of the liposome, but it will cause its stability to deteriorate. This is because when the content of matrine is low, it cannot form a eutectic with more glycyrrhetinic acid, which leads to more crystallization in the double fermented thick skin factor LAB POR composition, destroying the structure of the liposome, resulting in poor transdermal performance; too high a content of matrine will also lead to poor stability. The change in the pH conditions of the environment caused by the high content of matrine is the main factor affecting stability. Due to the decrease in stability, the retention amount of the skin becomes lower.

[0116] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A double fermented liposome containing thrush factor, characterized in that: Includes alkaloids, double fermented pachyderm factors, phospholipids and short chain fatty alcohols; The double fermentation thick skin factor comprises galactosyl yeast fermentation liquid, lactic acid bacteria NP and glycyrrhizic acid.

2. The double fermented pachyme liposome according to claim 1, characterized in that: In the double fermentation thick skin factor, the mass ratio of galactosyl yeast fermentation liquid, lactic acid bacteria NP and glycyrrhizic acid is (5-15):(40-60):

1.

3. The double fermented pachyme liposome according to claim 1, characterized in that The preparation method of the double fermentation thick skin factor is: The galactose yeast fermentation liquid, lactic acid bacteria NP and glycyrrhetinic acid are uniformly mixed and then homogenized for 1 to 3 times to obtain the double fermentation thick skin factor; Wherein, the homogenization pressure is 8000-10000psi.

4. The double fermented pachyme liposome according to any one of claims 1 to 3, characterized in that: According to the mass percentage, the invention comprises 0.2-2% of alkaloids, 6-55% of double fermented thick skin factor, 3-8% of phospholipids and 39-89% of short chain fatty alcohol.

5. The double fermented pachyme liposome according to any one of claims 1 to 3, characterized in that: The alkaloid comprises at least one of choline chloride, betaine and matrine, preferably matrine; Preferably, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propylene glycol, 1,3-propylene glycol, glycerol, butylene glycol, pentanediol and hexanediol, preferably butylene glycol or glycerol.

6. The double fermented pachyme liposome according to any one of claims 1 to 3, characterized in that: The phospholipids include soybean lecithin and / or hydrogenated lecithin, preferably soybean lecithin.

7. A method for preparing double fermented pachyme liposomes according to any one of claims 1 to 6, characterized in that: The following steps are involved: A. Evenly mixing the alkaloid and the double fermented pachyderm factor to obtain the double fermented pachyderm factor modified by the supramolecular eutectic solvent; B. dissolving phospholipids in short-chain fatty alcohols to obtain a phospholipid solution; C. adding the phospholipid solution to the double fermented pachyderm factor modified by the supramolecular eutectic solvent and mixing evenly to obtain a sample solution; D. homogenizing the sample solution to obtain double-fermented pachyme liposomes.

8. The preparation method according to claim 7, characterized in that: In step A, the mixing temperature is 50-70°C and the mixing time is 15-30 minutes; Preferably, in step B, the dissolving temperature is 70-90°C.

9. The preparation method according to claim 7, characterized in that: In step C, the temperature of the phospholipid solution is 45-55°C; Preferably, in step C, the mixing speed is 300-500 rpm and the mixing time is 10-15 min; Preferably, in step D, the homogenization pressure is 15000-25000 psi, the number of cycles is 1-5 times, and the discharge temperature is 10-30°C.

10. A cosmetic, characterized in that: The invention comprises the double fermented pachyme liposomes as described in any one of claims 1 to 6.

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