Skin antibacterial powder and preparation method thereof

Abstract

The invention discloses skin antibacterial powder and a preparation method thereof, and relates to the technical field of skin antibacterial products. Comprising the following components in parts by weight: 1-3 parts of chlorhexidine acetate nanocrystals, 5-8 parts of radix sophorae flavescentis powder, 6-10 parts of cortex phellodendri powder, 3-6 parts of cortex dictamni powder, 2-4 parts of sail powder, 1-3 parts of tea polyphenol / chitosan nanoparticles, 15-25 parts of other Chinese herbal medicine extracts, 0.8-1.5 parts of borneol powder and 0.6-1 part of plant polysaccharide. The skin antibacterial powder is good in antibacterial activity, good in antibacterial durability, low in toxic and side effects and remarkable in wound repairing effect.

Description

Technical Field

[0001] The invention relates to the technical field of skin antibacterial products, and in particular to skin antibacterial powder and a preparation method thereof. Background Art

[0002] As the first physiological defense line and the largest organ of the human body, the skin is always involved in the functional activities of the body. In daily life and work, there are a large number of bacteria and fungi in the environment we live in, and we may be infected if we are not careful. The skin is the main site for these pathogens to parasitize, so the skin is often more susceptible to invasion by a variety of bacteria, fungi, molds, viruses and other microorganisms. In particular, bacteria such as Staphylococcus aureus, Candida albicans, Escherichia coli and some fungi can cause skin irritation and accelerate skin infection. For fragile or susceptible skin, it may even cause pathological effects.

[0003] Skin antibacterial powder is a drug that can be used in the treatment of various skin diseases. It can play a conditioning and therapeutic role for various skin health problems. Since topical skin antibacterial powder can also exert a certain inhibitory and antagonistic effect on various dermatophytes, it can also play a good therapeutic effect when dealing with skin diseases caused by fungal infections. However, the existing skin antibacterial powder has poor bactericidal and antibacterial effects, treats a single disease or has a long course of treatment, which can lead to repeated skin attacks; in addition, most of the skin antibacterial powders on the market contain more chemical ingredients. For people with sensitive or delicate skin, they may experience symptoms such as itching, stinging, redness and swelling after using this product; in addition, if it contains antibiotic ingredients, it may also cause bacteria to develop drug resistance, affecting further treatment.

[0004] In order to solve the above problems, the Chinese invention patent with the authorization announcement number CN116173172B discloses an antibacterial powder for repairing wounds and its preparation method. The whole snail is fried or dried until it is yellow and fragile without carbonization, and then crushed at room temperature to obtain snail fine powder; it is mixed with borneol fine powder to obtain snail borneol fine powder for standby use; then it is evenly mixed with licorice powder, mulberry bark powder, angelica powder, and dried ginger powder, and irradiated to obtain antibacterial powder for repairing wounds, wherein the mass of borneol, licorice, mulberry bark, angelica and dried ginger is 0.1-20% of the mass of snail fine powder. The antibacterial powder described in the invention can promote faster healing of wounds, and has obvious antibacterial and regeneration ability to promote fresh tissue. The invention is a pure Chinese medicine powder developed by repeated experiments and research and development based on the ancient Chinese folk Chinese medicine prescription using modern biotechnology. It is efficient, safe, stable in performance, definite in efficacy, non-toxic and side effects, short in pain relief and recovery time, fast in onset, can effectively shorten the course of disease, and has good social and economic benefits. However, the antibacterial effect and antibacterial durability of the antibacterial powder still need to be further improved.

[0005] It can be seen that the art still needs a skin antibacterial powder with good antibacterial property, good antibacterial persistence, low toxicity and side effects, and significant wound repair effect, and a preparation method thereof. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and to provide a skin antibacterial powder with good antibacterial property, good antibacterial persistence, low toxicity and side effects, and significant wound repair effect, and a preparation method thereof.

[0007] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a skin antibacterial powder, comprising the following components by weight: 1-3 parts of chlorhexidine acetate nanocrystals, 5-8 parts of sophora flavescens powder, 6-10 parts of phellodendron chinense powder, 3-6 parts of Dictamni powder, 2-4 parts of Phellodendron chinense powder, 1-3 parts of tea polyphenols / chitosan nanoparticles, 15-25 parts of other Chinese herbal medicine extracts, 0.8-1.5 parts of borneol powder, and 0.6-1 parts of plant polysaccharides.

[0008] Preferably, the plant polysaccharide is at least one of Osmanthus fragrans polysaccharide and Ginkgo biloba polysaccharide.

[0009] Preferably, the other Chinese herbal extracts are extracted from the following raw materials in parts by weight: 3-5 parts of honeysuckle, 4-6 parts of cloves, 3-5 parts of azalea roots, 6-8 parts of isatis root, 5-8 parts of isatis indigotica, 6-10 parts of houttuynia cordata, 4-8 parts of wormwood, 4-6 parts of purslane, 5-8 parts of mulberry leaves, 6-8 parts of dandelion, 4-8 parts of turmeric, 5-8 parts of pomegranate peel, 4-6 parts of madder, 2-4 parts of epimedium, and 3-5 parts of angelica.

[0010] Preferably, the method for extracting the other Chinese herbal medicine extract comprises the following steps:

[0011] Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 1-3 hours, filtering and collecting the water extract and filter residue;

[0012] Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering;

[0013] Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

[0014] Preferably, in step S1, the mass ratio of the mixed material to water is 1:(6-9); and the decoction temperature is 95-98°C.

[0015] Preferably, the mass ratio of the filter residue to ethanol in step S2 is 1:(5-7); the extraction in step S2 is heating reflux extraction, the extraction is performed 3 times, each time for 1-2 hours, and the 3 extracts are combined and allowed to stand.

[0016] Preferably, there is no special requirement for the source of the tea polyphenol / chitosan nanoparticles. In one embodiment of the present invention, the tea polyphenol / chitosan nanoparticles are prepared according to the method of Example 1 in the authorization announcement number CN101856432B.

[0017] Preferably, the method for preparing the chlorhexidine acetate nanocrystals comprises the following steps:

[0018] Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution;

[0019] Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals;

[0020] Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

[0021] Preferably, the organic solvent in step D1 is at least one of glycerol and isopropanol; the stabilizer is at least one of hydroxypropyl methylcellulose E5, polyvinyl pyrrolidone K30, and Tween 80; the mass volume ratio of chlorhexidine acetate to the organic solvent is (0.08-0.15) g:1 mL; the mass volume ratio of the stabilizer to water is (50-150) mg:(30-50) mL.

[0022] Preferably, the volume ratio of the drug solution to the stabilizer solution in step D2 is 1:(30-60).

[0023] Preferably, the high-speed shearing conditions in step D2 are a shear stirring speed of 5000 to 10000 rpm and a shearing time of 10 to 15 min; the homogenization pressure of the high-pressure homogenization treatment is 12000 to 23000 psi and the number of homogenization times is 10 to 25 times.

[0024] Another object of the present invention is to provide a method for preparing the skin antibacterial powder, comprising the following steps: mixing the components evenly, grinding them through a 20-100 mesh sieve, and obtaining the skin antibacterial powder.

[0025] Due to the application of the above technical solution, the present invention has the following beneficial effects:

[0026] (1) The preparation method of the skin antibacterial powder disclosed in the present invention has simple process, convenient operation and control, low labor intensity, small impact on the environment, no need for special equipment, suitable for continuous large-scale production, and has high promotion and application value.

[0027] (2) The skin antibacterial powder disclosed in the present invention comprises the following components by weight: 1-3 parts of chlorhexidine acetate nanocrystals, 5-8 parts of sophora flavescens powder, 6-10 parts of phellodendron chinense powder, 3-6 parts of Dictamni cortex powder, 2-4 parts of Phellodendron chinense powder, 1-3 parts of tea polyphenol / chitosan nanoparticles, 15-25 parts of other Chinese herbal medicine extracts, 0.8-1.5 parts of borneol powder, and 0.6-1 parts of plant polysaccharides. Through the mutual cooperation and joint action of the components, the product can be endowed with excellent antibacterial properties and antibacterial persistence, effectively improving the wound repair effect, and the selected raw materials have low toxicity and side effects, little irritation to the skin, and are safer and more environmentally friendly to use.

[0028] (3) The skin antibacterial powder disclosed in the present invention uses chlorhexidine acetate nanocrystals to improve the stability and dissolution performance of chlorhexidine acetate, significantly improve its effect, thereby increasing the skin retention amount of chlorhexidine acetate when it is used externally; enhance the efficacy of the drug, reduce the frequency of drug use, and improve the effectiveness and safety of the drug; comply with the basic theory of pharmacoeconomics, and effectively improve the clinical value of the product.

[0029] (4) The skin antibacterial powder disclosed in the present invention, the other Chinese herbal extracts are extracted from the following raw materials by weight: 3-5 parts of honeysuckle, 4-6 parts of cloves, 3-5 parts of rhododendron roots, 6-8 parts of isatis root, 5-8 parts of isatis leaf, 6-10 parts of houttuynia, 4-8 parts of wormwood, 4-6 parts of purslane, 5-8 parts of mulberry leaves, 6-8 parts of dandelion, 4-8 parts of turmeric, 5-8 parts of pomegranate peel, 4-6 parts of madder, 2-4 parts of epimedium, 3-5 parts of angelica. Through the reasonable compatibility of various raw materials, the prepared product can stay well on the skin surface, inhibit bacteria for a long time and soothe the skin; through the synergistic effect of the combination of various raw materials, the advantages of good antibacterial effect, multiple antibacterial types and small addition amount can be achieved, so that the prepared product is safe, non-irritating and easy to absorb. DETAILED DESCRIPTION

[0030] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and those skilled in the art may think of other obvious variations.

[0031] Example 1

[0032] A skin antibacterial powder comprises the following components in parts by weight: 1 part of chlorhexidine acetate nanocrystals, 5 parts of sophora flavescens powder, 6 parts of phellodendron amurense powder, 3 parts of difficile cortex powder, 2 parts of scutellaria baicalensis powder, 1 part of tea polyphenol / chitosan nanoparticles, 15 parts of other Chinese herbal medicine extracts, 0.8 parts of borneol powder, and 0.6 parts of plant polysaccharides; the plant polysaccharides are osmanthus flavescentis polysaccharides.

[0033] The other Chinese herbal medicine extracts are extracted from the following raw materials in parts by weight: 3 parts of honeysuckle, 4 parts of cloves, 3 parts of azalea roots, 6 parts of isatis root, 5 parts of isatis indigotica, 6 parts of houttuynia, 4 parts of wormwood, 4 parts of purslane, 5 parts of mulberry leaves, 6 parts of dandelion, 4 parts of turmeric, 5 parts of pomegranate peel, 4 parts of madder, 2 parts of epimedium, and 3 parts of angelica.

[0034] The method for extracting the other Chinese herbal medicine extract comprises the following steps:

[0035] Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 1 hour, filtering and collecting the water extract and filter residue;

[0036] Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering;

[0037] Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

[0038] The mass ratio of the mixed material to water in step S1 is 1:6; the decoction temperature is 95°C; the mass ratio of the filter residue to ethanol in step S2 is 1:5; the extraction in step S2 is heating reflux extraction, the extraction is performed 3 times, each time for 1 hour, and the 3 extracts are combined and allowed to stand; the tea polyphenol / chitosan nanoparticles are prepared according to the method of Example 1 in the authorization announcement number CN101856432B.

[0039] The preparation method of the chlorhexidine acetate nanocrystal comprises the following steps:

[0040] Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution;

[0041] Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals;

[0042] Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

[0043] The organic solvent in step D1 is glycerol; the stabilizer is hydroxypropyl methylcellulose E5; the mass volume ratio of chlorhexidine acetate to the organic solvent is 0.08 g:1 mL; the mass volume ratio of the stabilizer to water is 50 mg:30 mL; and the volume ratio of the drug solution to the stabilizer solution in step D2 is 1:30.

[0044] The high-speed shearing conditions in step D2 are a shear stirring speed of 5000 rpm and a shearing time of 10 min; the homogenization pressure of the high-pressure homogenization treatment is 12000 psi and the number of homogenizations is 10 times.

[0045] A method for preparing the skin antibacterial powder comprises the following steps: mixing the components uniformly, grinding them through a 20-mesh sieve, and obtaining the skin antibacterial powder.

[0046] Example 2

[0047] A skin antibacterial powder comprises the following components in parts by weight: 1.5 parts of chlorhexidine acetate nanocrystals, 6 parts of sophora flavescens powder, 7 parts of phellodendron amurense powder, 4 parts of difficile cortex powder, 2.5 parts of scutellaria baicalensis powder, 1.5 parts of tea polyphenol / chitosan nanoparticles, 17 parts of other Chinese herbal medicine extracts, 1 part of borneol powder, and 0.7 parts of plant polysaccharides; the plant polysaccharides are ginkgo biloba polysaccharides.

[0048] The other Chinese herbal medicine extracts are extracted from the following raw materials in parts by weight: 3.5 parts of honeysuckle, 4.5 parts of cloves, 3.5 parts of azalea roots, 6.5 parts of isatis roots, 6 parts of isatis indigotica, 7 parts of houttuynia cordata, 5 parts of wormwood, 4.5 parts of purslane, 6 parts of mulberry leaves, 6.5 parts of dandelions, 5 parts of turmeric, 6 parts of pomegranate peel, 4.5 parts of madder, 2.5 parts of epimedium, and 3.5 parts of angelica.

[0049] The method for extracting the other Chinese herbal medicine extract comprises the following steps:

[0050] Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 1.5 hours, filtering and collecting the water extract and filter residue;

[0051] Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering;

[0052] Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

[0053] The mass ratio of the mixed material to water in step S1 is 1:7; the decoction temperature is 96°C; the mass ratio of the filter residue to ethanol in step S2 is 1:5.5; the extraction in step S2 is heating reflux extraction, the extraction is performed 3 times, each time for 1.2 hours, and the 3 extracts are combined and allowed to stand; the tea polyphenol / chitosan nanoparticles are prepared according to the method of Example 1 in the authorization announcement number CN101856432B.

[0054] The preparation method of the chlorhexidine acetate nanocrystal comprises the following steps:

[0055] Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution;

[0056] Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals;

[0057] Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

[0058] The organic solvent in step D1 is isopropanol; the stabilizer is polyvinyl pyrrolidone K30; the mass volume ratio of chlorhexidine acetate to the organic solvent is 0.12 g:1 mL; the mass volume ratio of the stabilizer to water is 90 mg:35 mL; the volume ratio of the drug solution to the stabilizer solution in step D2 is 1:40; the high-speed shearing conditions in step D2 are a shear stirring speed of 7000 rpm and a shear time of 12 min; the homogenization pressure of the high-pressure homogenization treatment is 14000 psi, and the number of homogenizations is 15 times.

[0059] A method for preparing the skin antibacterial powder comprises the following steps: mixing the components uniformly, grinding them through a 40-mesh sieve, and obtaining the skin antibacterial powder.

[0060] Example 3

[0061] A skin antibacterial powder, comprising the following components by weight: 2 parts of chlorhexidine acetate nanocrystals, 6.5 parts of sophora flavescens powder, 8 parts of phellodendron amurense powder, 4.5 parts of Dictamni cortex powder, 3 parts of Psoralea corylifolia powder, 2 parts of tea polyphenol / chitosan nanoparticles, 20 parts of other Chinese herbal medicine extracts, 1.3 parts of borneol powder, and 0.8 parts of plant polysaccharide; the plant polysaccharide is Osmanthus fragrans polysaccharide;

[0062] The other Chinese herbal medicine extracts are extracted from the following raw materials in parts by weight: 4 parts of honeysuckle, 5 parts of cloves, 4 parts of azalea roots, 7 parts of isatis root, 6.5 parts of isatis indigotica, 8 parts of houttuynia, 6 parts of wormwood, 5 parts of purslane, 6.5 parts of mulberry leaves, 7 parts of dandelion, 6 parts of turmeric, 6.5 parts of pomegranate peel, 5 parts of madder, 3 parts of epimedium, and 4 parts of angelica.

[0063] The method for extracting the other Chinese herbal medicine extract comprises the following steps:

[0064] Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 2 hours, filtering and collecting the water extract and filter residue;

[0065] Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering;

[0066] Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

[0067] The mass ratio of the mixed material to water in step S1 is 1:7.5; the decoction temperature is 97°C; the mass ratio of the filter residue to ethanol in step S2 is 1:6; the extraction in step S2 is heating reflux extraction, the extraction is performed 3 times, each time for 1.5 hours, and the 3 extracts are combined and allowed to stand; the tea polyphenol / chitosan nanoparticles are prepared according to the method of Example 1 in the authorization announcement number CN101856432B.

[0068] The preparation method of the chlorhexidine acetate nanocrystal comprises the following steps:

[0069] Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution;

[0070] Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals;

[0071] Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

[0072] The organic solvent in step D1 is isopropanol; the stabilizer is Tween 80; the mass volume ratio of chlorhexidine acetate to the organic solvent is 0.13 g:1 mL; the mass volume ratio of the stabilizer to water is 100 mg:40 mL; the volume ratio of the drug solution to the stabilizer solution in step D2 is 1:45; the high-speed shearing conditions in step D2 are a shear stirring speed of 7500 rpm and a shear time of 13 min; the homogenization pressure of the high-pressure homogenization treatment is 18000 psi, and the number of homogenizations is 18 times.

[0073] A method for preparing the skin antibacterial powder comprises the following steps: mixing the components uniformly, grinding them through an 80-mesh sieve, and obtaining the skin antibacterial powder.

[0074] Example 4

[0075] A skin antibacterial powder comprises the following components in parts by weight: 2.5 parts of chlorhexidine acetate nanocrystals, 7.5 parts of sophora flavescens powder, 9.5 parts of phellodendron amurense powder, 5.5 parts of difficile cortex powder, 3.5 parts of scutellaria baicalensis powder, 2.5 parts of tea polyphenol / chitosan nanoparticles, 23 parts of other Chinese herbal medicine extracts, 1.4 parts of borneol powder, and 0.9 parts of plant polysaccharides; the plant polysaccharides are prepared by mixing osmanthus fragrans polysaccharides and ginkgo biloba polysaccharides in a mass ratio of 1:3.

[0076] The other Chinese herbal medicine extracts are extracted from the following raw materials in parts by weight: 4.5 parts of honeysuckle, 5.5 parts of cloves, 4.5 parts of azalea roots, 7.5 parts of isatis roots, 7.5 parts of isatis indigotica, 9.5 parts of houttuynia cordata, 7.5 parts of wormwood, 5.5 parts of purslane, 7.5 parts of mulberry leaves, 7.5 parts of dandelions, 7.5 parts of turmeric, 7.5 parts of pomegranate peel, 5.5 parts of madder, 3.5 parts of epimedium, and 4.5 parts of angelica.

[0077] The method for extracting the other Chinese herbal medicine extract comprises the following steps:

[0078] Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 2.5 hours, filtering and collecting the water extract and filter residue;

[0079] Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering;

[0080] Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

[0081] The mass ratio of the mixed material to water in step S1 is 1:8.5; the decoction temperature is 97°C; the mass ratio of the filter residue to ethanol in step S2 is 1:6.5; the extraction in step S2 is heating reflux extraction, the extraction is performed 3 times, each time for 1.8 hours, and the 3 extracts are combined and allowed to stand; the tea polyphenol / chitosan nanoparticles are prepared according to the method of Example 1 in the authorization announcement number CN101856432B.

[0082] The preparation method of the chlorhexidine acetate nanocrystal comprises the following steps:

[0083] Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution;

[0084] Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals;

[0085] Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

[0086] The organic solvent in step D1 is a mixture of glycerol and isopropanol in a mass ratio of 3:5; the stabilizer is a mixture of hydroxypropyl methylcellulose E5, polyvinyl pyrrolidone K30 and Tween 80 in a mass ratio of 1:2:3; the mass volume ratio of chlorhexidine acetate to the organic solvent is 0.14g:1mL; the mass volume ratio of the stabilizer to water is 140mg:45mL; the volume ratio of the drug solution to the stabilizer solution in step D2 is 1:55; the high-speed shear condition in step D2 is a shear stirring speed of 9000rpm and a shear time of 14min; the homogenization pressure of the high-pressure homogenization treatment is 22000psi, and the number of homogenizations is 23 times.

[0087] A method for preparing the skin antibacterial powder comprises the following steps: mixing the components uniformly, grinding them through a 90-mesh sieve, and obtaining the skin antibacterial powder.

[0088] Example 5

[0089] A skin antibacterial powder comprises the following components in parts by weight: 3 parts of chlorhexidine acetate nanocrystals, 8 parts of sophora flavescens powder, 10 parts of phellodendron amurense powder, 6 parts of difficile cortex powder, 4 parts of scutellaria baicalensis powder, 3 parts of tea polyphenol / chitosan nanoparticles, 25 parts of other Chinese herbal medicine extracts, 1.5 parts of borneol powder, and 1 part of plant polysaccharide; the plant polysaccharide is osmanthus fragrans polysaccharide.

[0090] The other Chinese herbal medicine extracts are extracted from the following raw materials in parts by weight: 5 parts of honeysuckle, 6 parts of cloves, 5 parts of azalea roots, 8 parts of isatis root, 8 parts of isatis indigotica, 10 parts of houttuynia cordata, 8 parts of wormwood, 6 parts of purslane, 8 parts of mulberry leaves, 8 parts of dandelions, 8 parts of turmeric, 8 parts of pomegranate peels, 6 parts of madder, 4 parts of epimedium, and 5 parts of angelica.

[0091] The method for extracting the other Chinese herbal medicine extract comprises the following steps:

[0092] Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 3 hours, filtering and collecting the water extract and filter residue;

[0093] Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering;

[0094] Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

[0095] The mass ratio of the mixed material to water in step S1 is 1:9; the decoction temperature is 98°C; the mass ratio of the filter residue to ethanol in step S2 is 1:7; the extraction in step S2 is heating reflux extraction, the extraction is performed 3 times, each time for 2 hours, and the 3 extracts are combined and allowed to stand; the tea polyphenol / chitosan nanoparticles are prepared according to the method of Example 1 in the authorization announcement number CN101856432B.

[0096] The preparation method of the chlorhexidine acetate nanocrystal comprises the following steps:

[0097] Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution;

[0098] Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals;

[0099] Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

[0100] The organic solvent in step D1 is glycerol; the stabilizer is hydroxypropyl methylcellulose E5; the mass volume ratio of chlorhexidine acetate to the organic solvent is 0.15g:1mL; the mass volume ratio of the stabilizer to water is 150mg:50mL; the volume ratio of the drug solution to the stabilizer solution in step D2 is 1:60; the high-speed shearing conditions in step D2 are a shear stirring speed of 10000rpm and a shear time of 15min; the homogenization pressure of the high-pressure homogenization treatment is 23000psi, and the number of homogenizations is 25 times.

[0101] A method for preparing the skin antibacterial powder comprises the following steps: mixing the components uniformly, grinding them through a 100-mesh sieve, and obtaining the skin antibacterial powder.

[0102] Comparative Example 1

[0103] A skin antibacterial powder is basically the same as Example 1, except that an equal amount of chlorhexidine acetate is used instead of chlorhexidine acetate nanocrystals, and no plant polysaccharide is added.

[0104] Comparative Example 2

[0105] A skin antibacterial powder is basically the same as Example 1, except that tea polyphenol / chitosan nanoparticles, pomegranate peel, madder and epimedium are not added.

[0106] In order to further illustrate the beneficial technical effects of the skin antibacterial powder involved in each embodiment of the present invention, the skin antibacterial powder involved in Examples 1-5 and Comparative Examples 1-2 was tested for relevant performances. The test results are shown in Table 1. The test method is as follows:

[0107] (1) Skin irritation test: Before the test, the hair on both sides of the midline of the rabbit's back was cut or shaved (an area of ​​about 3 × 5 cm 2 ), carefully examine the skin, select rabbits with intact skin, 5 in each group, and evenly apply the test sample on the left hair-free area of ​​the experimental animals at a coating amount of 2.0 mg / cm 2 , and cover with oil paper and two layers of gauze, and then fix it with non-irritating tape or bandage to ensure close contact between the test sample and the skin, prevent shedding and animal licking of the test sample. Apply 45% ethanol evenly to the hair removal area on the right side, and the rest of the operation is the same as the left side. Remove the fixation and covering 24 hours after application, wash off the residual test sample on the skin with warm water or appropriate solvent, and observe the local skin lesions.

[0108] (2) Skin allergy test: Before the test, cut or shave the hair on both sides of the midline of the guinea pig's back (area of ​​about 3 × 3 cm 2 ), carefully examine the skin, select guinea pigs with intact skin, 5 in each group. Apply the test sample evenly on the hair removal area of ​​the experimental animals, with a coating amount of 2.0 mg / cm 2 , and covered with oil paper and two layers of gauze, and then fixed with non-irritating tape or bandage to ensure close contact between the test sample and the skin to prevent shedding and animal licking. 24 hours after the test sample was applied, the fixation and covering were removed, and the residual test sample on the skin was washed off with warm water or appropriate solvent, and the local skin lesions were observed; for the negative group, 45% ethanol was evenly applied to the hair removal area. The skin allergic reaction was observed.

[0109] (3) Antibacterial test: Common bacteria on the body surface, such as Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecium, and Candida albicans, were used as bacterial species. The test steps are as follows:

[0110] Step 1: Prepare freshly cultured bacteria (Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecium, Candida albicans) for 20 h, wash the bacterial lawn with 5 mL of PBS solution (0.03 mol / L) to prepare a bacterial suspension, and dilute it with PBS to the required concentration (drop 100 μL on the control sample, and recover 1×10 4 -9×10 4 cfu / piece);

[0111] Step 2, weigh a certain amount of test samples and disperse them in PBS to prepare a sample solution (concentration of 2000ppm), and put them into a 250ml conical flask;

[0112] Step 3, fix the conical flask on a shaking table and shake at 300 r / min for 1 h;

[0113] Step 4: Take 0.5 mL of the sample solution at time 0 and after shaking for 1 h, inoculate the plate by the agar pouring method, and count the colonies after culturing in a 36°C incubator for 22 hours.

[0114] The test was repeated 3 times, and the antibacterial rate was calculated according to the formula: X = (AB) / A × 100%,

[0115] Where: X-antibacterial rate, %; A-average colony count of the test sample before oscillation; B-average colony count of the test sample after oscillation.

[0116] (4) Wound repair effect experiment: 14 mice were randomly divided into 7 groups, with 2 mice in each group. Two symmetrical parts were taken from the back of the mice with the spine as the center. After shaving, the skin was disinfected with iodine. The skin surface was polished with 100-mesh sandpaper to make the skin surface damaged and bleeding. The products of Examples 1-5 and Comparative Examples 1-2 were applied twice a day in the morning and evening, with an application amount of 2.0 mg / cm each time. 2 Wound area and wound condition were observed after 2 days.

[0117] Table 1

[0118]

[0119] As can be seen from Table 1, the skin antibacterial powder involved in the embodiment of the present invention has better antibacterial properties than the comparative example product, and has better wound repair effect, is non-irritating and non-sensitizing to the skin; the use of chlorhexidine acetate nanocrystals, plant polysaccharides, tea polyphenols / chitosan nanoparticles, pomegranate peel, madder and epimedium is beneficial to improving the above properties.

[0120] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with this technology to understand the contents of the present invention and implement them accordingly. They cannot be used to limit the protection scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin antibacterial powder, characterized in that: The invention comprises the following components in parts by weight: 1-3 parts of chlorhexidine acetate nanocrystals, 5-8 parts of sophora flavescens powder, 6-10 parts of phellodendron amurense powder, 3-6 parts of difficile cortex powder, 2-4 parts of smilax glabra powder, 1-3 parts of tea polyphenol / chitosan nanoparticles, 15-25 parts of other Chinese herbal medicine extracts, 0.8-1.5 parts of borneol powder and 0.6-1 parts of plant polysaccharides.

2. The skin antibacterial powder according to claim 1, characterized in that: The plant polysaccharide is at least one of Osmanthus fragrans polysaccharide and Ginkgo biloba polysaccharide.

3. The skin antibacterial powder according to claim 1, characterized in that: The other Chinese herbal medicine extracts are extracted from the following raw materials in parts by weight: 3-5 parts of honeysuckle, 4-6 parts of cloves, 3-5 parts of azalea roots, 6-8 parts of isatis root, 5-8 parts of isatis indigotica, 6-10 parts of houttuynia cordata, 4-8 parts of wormwood, 4-6 parts of purslane, 5-8 parts of mulberry leaves, 6-8 parts of dandelion, 4-8 parts of turmeric, 5-8 parts of pomegranate peel, 4-6 parts of madder, 2-4 parts of epimedium, and 3-5 parts of angelica.

4. The skin antibacterial powder according to claim 3, characterized in that: The method for extracting the other Chinese herbal medicine extract comprises the following steps: Step S1, uniformly mixing the raw materials of other Chinese herbal medicine extracts to obtain a mixed material, adding water and boiling for 1-3 hours, filtering and collecting the water extract and filter residue; Step S2, extracting the filter residue with ethanol, and collecting the alcohol extract by filtering; Step S3, combining the above two extracts, concentrating under reduced pressure to recover ethanol and water, and obtaining other Chinese herbal medicine extracts.

5. The skin antibacterial powder according to claim 4, characterized in that: In step S1, the mass ratio of the mixed material to water is 1:(6-9); the decoction temperature is 95-98°C.

6. The skin antibacterial powder according to claim 4, characterized in that: The mass ratio of the filter residue to ethanol in step S2 is 1:(5-7); the extraction in step S2 is heating reflux extraction, which is performed 3 times, each time for 1-2 hours, and the 3 extracts are combined and allowed to stand.

7. The skin antibacterial powder according to claim 1, characterized in that: The preparation method of the chlorhexidine acetate nanocrystal comprises the following steps: Step D1, dissolving chlorhexidine acetate nanocrystals in an organic solvent to obtain a drug solution; dissolving a stabilizer in water to obtain a stabilizer solution; Step D2, adding the drug solution to the stabilizer solution, and performing high-speed shear dispersion to obtain a crude drug suspension; performing high-pressure homogenization on the crude drug suspension to obtain a suspension of chlorhexidine acetate nanocrystals; Step D3, spray drying the chlorhexidine acetate nanocrystal suspension to obtain chlorhexidine acetate nanocrystals.

8. The skin antibacterial powder according to claim 7, characterized in that: The organic solvent in step D1 is at least one of glycerol and isopropanol; the stabilizer is at least one of hydroxypropyl methylcellulose E5, polyvinyl pyrrolidone K30, and Tween 80; the mass volume ratio of chlorhexidine acetate to the organic solvent is (0.08-0.15) g:1 mL; the mass volume ratio of the stabilizer to water is (50-150) mg:(30-50) mL.

9. The skin antibacterial powder according to claim 7, characterized in that: The volume ratio of the drug solution to the stabilizer solution in step D2 is 1:(30-60); the high-speed shearing condition in step D2 is a shear stirring speed of 5000-10000 rpm and a shearing time of 10-15 min; the homogenization pressure of the high-pressure homogenization treatment is 12000-23000 psi, and the number of homogenization times is 10-25 times.

10. A method for preparing the skin antibacterial powder according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: mixing the components uniformly, grinding the components through a 20-100 mesh sieve, and obtaining skin antibacterial powder.

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