Skin cleaning composition and application thereof in cleaning cream

Abstract

The invention relates to a skin-cleaning composition and application thereof in cleaning cream, and belongs to the technical field of daily chemicals, the skin-cleaning composition comprises the following raw materials: plant fermentation liquor, a chrysanthemum artemisia extract, silk amino acid, cetyl alcohol ethyl hexanoate, PEG-20 glyceryl triisostearate and soapberry seed oil; the preparation method of the plant fermentation liquid comprises the following steps: S1, pretreating part of the raw materials; s2, activating and culturing the strain; and S3, adjusting the pH value of the mixed solution A, performing temperature-controlled sterilization, cooling, adding mannase, performing temperature-controlled enzymolysis, performing enzyme deactivation after enzymolysis is completed, so as to obtain a mixed solution B, cooling, adding a lactobacillus acidophilus fermentation seed solution and a streptococcus thermophilus fermentation seed solution, performing temperature-controlled standing fermentation, performing temperature-controlled sterilization and speed-controlled centrifugation after fermentation is completed, taking supernate, and performing 0.22 mu m membrane microfiltration, so as to obtain the plant fermentation liquor. The raw materials of the skin-cleaning composition provided by the invention have a synergistic effect, so that the skin-cleaning composition has the effects of moisturizing and whitening while effectively cleaning the skin.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemicals, and in particular relates to a skin cleansing composition and application thereof in a cleansing cream. Background Art

[0002] With the acceleration of modern life and the intensification of environmental pollution, skin problems have become the focus of people's attention. As the largest organ of the human body, the skin not only has the function of protecting the internal organs of the body, but also plays an important role in regulating body temperature and sensing external stimuli. However, factors such as long-term ultraviolet radiation, air pollution and bad living habits can cause problems such as excessive oil secretion, dullness and dryness of the skin. The main function of cleansing cream is to deeply clean the skin and remove dirt, oil and makeup residue on the skin surface. Although using cleansing cream after makeup can effectively remove makeup, cleansing cream is also suitable for daily skin cleaning. It can penetrate deep into the pores, remove dirt and excess oil in the pores, and help maintain the normal metabolism and breathing of the skin; at the same time, some high-quality cleansing creams also contain moisturizing and nourishing ingredients, which can provide the skin with necessary nutrition and moisture while cleaning, and keep the skin healthy. There are very few compositions on the market that can have multiple functions at the same time. Traditional compositions used for cleansing creams mainly focus on the cleaning function, dissolving oil and makeup on the skin surface through oil components, but it is often difficult to take into account both whitening and hydration.

[0003] Therefore, it is of great practical significance to develop a composition that combines skin cleansing, whitening and moisturizing, and apply it to cleansing cream products to meet consumers' needs for comprehensive skin care. Summary of the invention

[0004] The purpose of the present invention is to provide a skin cleansing composition and its application in a cleansing cream, which solves the problem in the prior art that the skin cleansing composition has both moisturizing and whitening effects while cleansing the skin*.

[0005] The purpose of the present invention can be achieved through the following technical solutions: A skin cleansing composition, the skin cleansing composition specifically comprises the following raw materials in parts by weight: 4-6 parts by weight of plant fermentation liquid, 7-10 parts by weight of Artemisia selengensis extract, 15-22 parts by weight of silk amino acids, 44-52 parts by weight of cetyl ethylhexanoate, 20-26 parts by weight of PEG-20 glyceryl triisostearate, and 50-60 parts by weight of solvent; The method for preparing the plant fermentation liquid comprises the following steps: S1, pre-treating part of the raw materials to obtain a mixed solution A; S2, activating and culturing the strains to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid; S3, adjusting the pH of the mixed solution A, sterilizing under controlled temperature, cooling, adding mannanase to perform temperature-controlled enzymolysis, inactivating the enzyme after the enzymolysis is completed to obtain a mixed solution B, cooling, adding Lactobacillus acidophilus fermentation seed solution and thermophilic Streptococcus fermentation seed solution to perform temperature-controlled static fermentation, sterilizing under controlled temperature after the fermentation is completed, centrifuging under controlled speed, taking the supernatant and filtering it through a 0.22 μm membrane to obtain a plant fermentation liquid.

[0006] Furthermore, the chrysanthemum extract is obtained by using the whole herb of Tanacetum vulgare, a herbaceous plant of the genus Tanacetum of the Asteraceae family, as a raw material, using deionized water as a solvent, and subjecting the whole herb to conventional reflux extraction at a solid-liquid ratio of 1:15. The chrysanthemum extract contains chrysanthemum acid, flavonoid compounds and gallic acid components, which can promote the production of hyaluronic acid synthase, thereby replenishing moisture to the skin. The silk amino acids (SILK AMINO ACIDS), CAS: 96692-41-4, were purchased from Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.; cetyl ethylhexanoate, CAS: 59130-69-7, was purchased from Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.; the PEG-20 glyceryl triisostearate was purchased from Shenzhen Xingkaiyue Biotechnology Co., Ltd.

[0007] As a preferred technical solution of the present invention, step S1 specifically includes: air-drying, crushing and sieving Tricholoma matsutake and Cuscuta chinensis to obtain Tricholoma matsutake powder and Cuscuta chinensis powder, uniformly mixing Tricholoma matsutake powder, Cuscuta chinensis powder and deionized water, controlling the temperature and sterilizing to obtain a mixed solution A for standby use.

[0008] Tricholoma matsutake belongs to the genus Tricholoma of the family Trichostomaceae. It is rich in chemical components such as polysaccharides and polypeptides, which can stably and effectively inhibit the activity of tyrosinase, achieving the effect of whitening and removing spots; Cuscutachinensis Lam. is the dried mature seeds of an annual parasitic herb of the genus Cuscutachin of the family Convolvulaceae, mainly including chemical components such as polysaccharides, flavonoids, polyphenols and sterols, which can significantly reduce the production of melanin in B16 cells.

[0009] Furthermore, the mass ratio of the Tricholoma matsutake powder to the Cuscuta chinensis powder is 2-3:1.5-3; and the amount of the deionized water added is 100-110 times the total mass of the Tricholoma matsutake powder and the Cuscuta chinensis powder.

[0010] Furthermore, the screening is through a 50-60 mesh sieve; the temperature of the temperature-controlled sterilization is 121° C., and the time is 20 minutes.

[0011] As a preferred technical solution of the present invention, step S2 specifically includes: inoculating Lactobacillus acidophilus and Streptococcus thermophilus into MRS liquid culture medium respectively and culturing at controlled temperature once to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus, inoculating activated Lactobacillus acidophilus and activated Streptococcus thermophilus into MRS liquid culture medium at an inoculum amount of 3-5% by volume, respectively, culturing at controlled temperature twice to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid.

[0012] Furthermore, the Lactobacillus acidophilus: item number xyh001, was purchased from Hubei Xinyuhong Biomedicine Technology Co., Ltd.; the Streptococcus thermophilus was purchased from Guangdong Hongyou Biotechnology Co., Ltd.

[0013] Furthermore, the first temperature-controlled culture is: temperature-controlled at 37-42°C for 32-40 hours; the second temperature-controlled culture is: temperature-controlled at 37-42°C for 32-40 hours until the concentration of the fermented Lactobacillus acidophilus seed liquid is 2-2.1×10 8 CFU / mL, the concentration of thermophilic Streptococcus fermentation seed liquid is 1.8-2×10 8 CFU / mL.

[0014] As a preferred technical solution of the present invention, the mass ratio of the mixed solution A to the mannanase in step S3 is 1:0.002-0.003; the mass ratio of the mixed solution B, the fermentation seed solution of Lactobacillus acidophilus and the fermentation seed solution of Streptococcus thermophilus is 1:0.003-0.005:0.002-0.003.

[0015] Furthermore, the enzymatic activity of the mannanase includes 1×10 4 U / g.

[0016] As a preferred technical solution of the present invention, in step S3, the pH of the mixed solution A is adjusted to 7.0-7.5 using 0.1 mol / L sodium hydroxide solution; the temperature of the temperature-controlled sterilization is 121°C, and the time is 15 min; the cooling is cooled to room temperature; the temperature of the temperature-controlled enzymatic hydrolysis is 55-60°C, and the time is 2-3 h; the enzyme inactivation is inactivation in a boiling water bath for 20-30 min; the temperature of the temperature-controlled static fermentation is 37-40°C, and the time is 48 h; the speed of the speed-controlled centrifugation is 8000-9000 r / min, and the time is 20-30 min.

[0017] As a preferred technical solution of the present invention, the solvent includes ethanol.

[0018] As a preferred technical solution of the present invention, the skin cleansing composition also includes soapberry seed oil.

[0019] Furthermore, the soapberry seed oil is 1-1.6 parts by weight.

[0020] Furthermore, soapberry seed oil is obtained by taking the dried seeds of SCHLEICHERA TRIJUGA as raw materials, using n-hexane as solvent, and subjecting to conventional Soxhlet reflux extraction at a solid-liquid ratio of 1:20; soapberry seed oil mainly contains oleic acid, cis-11-eicosenoic acid, linoleic acid, arachidic acid and other ingredients, and has excellent skin cleansing and moisturizing effects.

[0021] A preparation method of a skin cleansing composition specifically comprises the following steps: mixing cetyl ethylhexanoate, PEG-20 glyceryl triisostearate and a solvent evenly under temperature control, then adding the remaining raw materials, mixing and stirring evenly to obtain a finished skin cleansing composition.

[0022] As a preferred technical solution of the present invention, the temperature of the temperature control is 30-35°C.

[0023] An application of a skin cleansing composition, wherein the skin cleansing composition is added into skin care products at a mass percentage of 3-5%.

[0024] As a preferred technical solution of the present invention, the skin care products include but are not limited to cleansing cream, facial cleanser, and cleansing paste.

[0025] Beneficial effects of the present invention: (1) The present invention uses Tricholoma matsutake and Cuscuta chinensis as raw materials to inhibit tyrosinase activity and reduce the amount of melanin produced in B16 cells, and synergistically makes the prepared composition have a certain whitening effect; at the same time, on this basis, the present invention first uses mannanase for enzymatic hydrolysis and then uses Lactobacillus acidophilus and Streptococcus thermophilus to co-ferment Tricholoma matsutake and Cuscuta chinensis, so that mannanase increases the content of polysaccharide components in the system, thereby playing a better auxiliary role in the process of inhibiting tyrosinase in the composition, thermophilic Streptococcus degrades the macromolecular substances in the system into small molecular substances, and Lactobacillus acidophilus releases the polypeptide components in the system; therefore, under the enzymatic hydrolysis and fermentation process of the present invention, Tricholoma matsutake and Cuscuta chinensis can greatly assist in enhancing the whitening effect of the finished composition.

[0026] (2) The present invention compounds cetyl ethylhexanoate and PEG-20 glyceryl triisostearate, and takes advantage of the two different aspects of similar miscibility of oils and fats and emulsification and encapsulation, so that the prepared finished composition has strong skin cleansing ability. In addition, the soapberry seed oil added in the present invention also has certain cleansing effect as a natural plant oil, which can assist cetyl ethylhexanoate in strengthening its oil-dissolving effect and thus strengthening the skin cleansing function of the prepared finished composition.

[0027] (3) The present invention adds an Artemisia selengensis extract containing artemisinic acid, flavonoid compounds and gallic acid to promote the production of hyaluronic acid synthase, thereby replenishing moisture for the skin; adds soapberry seed oil mainly containing oleic acid, cis-11-eicosenoic acid, linoleic acid and other ingredients to moisturize the skin; adds silk amino acids with highly hydrophilic properties to effectively stay on the skin to form a film with protective and moisturizing effects, thereby locking in moisture for the skin; therefore, the Artemisia selengensis extract, soapberry seed oil and silk amino acids added in the present invention can synergistically replenish moisture, moisturize the skin and lock in moisture, so that the prepared composition has an outstanding moisturizing effect.

[0028] (4) The skin cleansing composition provided by the present invention can not only effectively cleanse the skin, but also fully hydrate the skin and firmly lock in moisture, thereby maintaining the skin's moisture state and preventing the skin from becoming dry and tight; in addition, the composition also has excellent whitening effects, which can effectively improve the skin's color and enhance the overall health and beauty of the skin. DETAILED DESCRIPTION

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The tanacetum extract used in the embodiment of the present invention is obtained by using the whole herb of Tanacetum vulgare, a herbaceous plant of the genus Tanacetum of the Asteraceae family, as a raw material, using deionized water as a solvent, and subjecting it to conventional reflux extraction at a solid-liquid ratio of 1:15; the silk amino acids (SILK AMINO ACIDS) and cetyl ethylhexanoate used are purchased from Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.; the PEG-20 glyceryl triisostearate used is purchased from Shenzhen Xingkaiyue Biotechnology Co., Ltd.; the Lactobacillus acidophilus used: item number xyh001, purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd.; the thermophilic Streptococcus used is purchased from Guangdong Hongyou Biotechnology Co., Ltd.; the enzyme activity of the mannanase used includes 1×104 U / g; the soapberry seed oil used is obtained by using the dried seeds of SCHLEICHERA TRIJUGA as a raw material, using n-hexane as a solvent, and subjecting it to conventional Soxhlet reflux extraction at a solid-liquid ratio of 1:20; the above will not be repeated later.

[0031] Example 1 Prepare plant fermentation liquid, the preparation method of the plant fermentation liquid comprises the following steps: S1, air-drying and crushing Tricholoma matsutake and Cuscuta chinensis, passing through a 50-mesh sieve to obtain Tricholoma matsutake powder and Cuscuta chinensis powder, uniformly mixing Tricholoma matsutake powder, Cuscuta chinensis powder and deionized water, and sterilizing at 121° C. for 20 min to obtain a mixed solution A for standby use; The mass ratio of the Matsutake mushroom powder to the Cuscuta seeds powder is 2.5:1.5; the amount of the deionized water added is 105 times the total mass of the Matsutake mushroom powder and the Cuscuta seeds powder; S2, Lactobacillus acidophilus and Streptococcus thermophilus were inoculated into MRS liquid culture medium, respectively, and the temperature was controlled at 37°C for 36 hours to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus. The activated Lactobacillus acidophilus and activated Streptococcus thermophilus were inoculated into MRS liquid culture medium at a volume fraction of 3% and the temperature was controlled at 37°C for a second time to culture the Lactobacillus acidophilus fermentation seed liquid at a concentration of 2×10 8 CFU / mL, the concentration of thermophilic Streptococcus fermentation seed liquid was 1.8×10 8 CFU / mL; S3, using 0.1mol / L sodium hydroxide solution to adjust the pH of the mixed solution A to 7.0, control the temperature at 121°C for sterilization for 15min, cool to room temperature, add mannanase to control the temperature at 55°C for enzymolysis for 2h, after the enzymolysis is completed, inactivate the enzyme in a boiling water bath for 25min to obtain a mixed solution B, cool, add Lactobacillus acidophilus fermentation seed liquid and thermophilic Streptococcus fermentation seed liquid to control the temperature at 37°C and ferment for 48h, after the fermentation is completed, control the temperature at 121°C for sterilization for 15min, control the speed at 9000r / min for centrifugation for 30min, take the supernatant and filter it through a 0.22μm membrane to obtain a plant fermentation liquid; The mass ratio of the mixed solution A to mannanase is 1:0.0025; the mass ratio of the mixed solution B, Lactobacillus acidophilus fermentation seed solution and Streptococcus thermophilus fermentation seed solution is 1:0.003:0.0025.

[0032] Example 2 Prepare plant fermentation liquid, the preparation method of the plant fermentation liquid comprises the following steps: S1, air-drying and crushing Tricholoma matsutake and Cuscuta chinensis, passing through a 60-mesh sieve to obtain Tricholoma matsutake powder and Cuscuta chinensis powder, uniformly mixing Tricholoma matsutake powder, Cuscuta chinensis powder and deionized water, and sterilizing at 121° C. for 20 min to obtain a mixed solution A for standby use; The mass ratio of the Matsutake mushroom powder to the Cuscuta chinensis powder is 3:2.2; the amount of the deionized water added is 100 times the total mass of the Matsutake mushroom powder and the Cuscuta chinensis powder; S2, Lactobacillus acidophilus and Streptococcus thermophilus were inoculated into MRS liquid culture medium, respectively, and the temperature was controlled at 42°C for 32 hours to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus. The activated Lactobacillus acidophilus and activated Streptococcus thermophilus were inoculated into MRS liquid culture medium at a volume fraction of 5%, and the temperature was controlled at 42°C for a second time to culture the Lactobacillus acidophilus fermentation seed liquid at a concentration of 2.1×10 8 CFU / mL, the concentration of thermophilic Streptococcus fermentation seed liquid was 2×10 8 CFU / mL; S3, using 0.1mol / L sodium hydroxide solution to adjust the pH of the mixed solution A to 7.5, sterilize at 121°C for 15min, cool to room temperature, add mannanase to control the temperature at 60°C for enzymolysis for 2.5h, and after the enzymolysis is completed, inactivate the enzyme in a boiling water bath for 20min to obtain a mixed solution B, cool, add Lactobacillus acidophilus fermentation seed liquid and thermophilic Streptococcus fermentation seed liquid to control the temperature at 40°C and ferment for 48h, after the fermentation is completed, control the temperature at 121°C for sterilization for 15min, control the speed at 8000r / min for centrifugation for 20min, take the supernatant and filter it through a 0.22μm membrane to obtain a plant fermentation liquid; The mass ratio of the mixed solution A to mannanase is 1:0.003; the mass ratio of the mixed solution B, Lactobacillus acidophilus fermentation seed solution and Streptococcus thermophilus fermentation seed solution is 1:0.004:0.003.

[0033] Example 3 Prepare plant fermentation liquid, the preparation method of the plant fermentation liquid comprises the following steps: S1, air-drying and crushing Tricholoma matsutake and Cuscuta chinensis, passing through a 50-mesh sieve to obtain Tricholoma matsutake powder and Cuscuta chinensis powder, uniformly mixing Tricholoma matsutake powder, Cuscuta chinensis powder and deionized water, and sterilizing at 121° C. for 20 min to obtain a mixed solution A for standby use; The mass ratio of the matsutake mushroom powder to the dodder powder is 2:3; the amount of the deionized water added is 110 times the total mass of the matsutake mushroom powder and the dodder powder; S2, Lactobacillus acidophilus and Streptococcus thermophilus were inoculated into MRS liquid culture medium, respectively, and the temperature was controlled at 40°C for 40 hours to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus. The activated Lactobacillus acidophilus and activated Streptococcus thermophilus were inoculated into MRS liquid culture medium at a volume fraction of 4%, and the temperature was controlled at 40°C for a second time to culture the Lactobacillus acidophilus fermentation seed liquid at a concentration of 2×10 8 CFU / mL, the concentration of thermophilic Streptococcus fermentation seed liquid was 2×10 8 CFU / mL; S3, using 0.1mol / L sodium hydroxide solution to adjust the pH of the mixed solution A to 7.3, sterilize at 121°C for 15min, cool to room temperature, add mannanase to control the temperature at 57.5°C for enzymolysis for 3h, and after the enzymolysis is completed, inactivate the enzyme in a boiling water bath for 30min to obtain a mixed solution B, cool, add Lactobacillus acidophilus fermentation seed liquid and thermophilic Streptococcus fermentation seed liquid to control the temperature at 38.5°C and ferment for 48h, after the fermentation is completed, control the temperature at 121°C for sterilization for 15min, control the speed at 8500r / min for centrifugation for 25min, take the supernatant and filter it through a 0.22μm membrane to obtain a plant fermentation liquid; The mass ratio of the mixed solution A to mannanase is 1:0.002; the mass ratio of the mixed solution B, Lactobacillus acidophilus fermentation seed solution and Streptococcus thermophilus fermentation seed solution is 1:0.005:0.002.

[0034] Comparative Example 1 Compared with Example 2, the difference is that in step S1 of Comparative Example 1, no Tricholoma matsutake powder is added, and Tricholoma matsutake powder is used instead, and the remaining operating steps and parameters are the same.

[0035] Comparative Example 2 Compared with Example 2, the difference is that in step S1 of Comparative Example 2, dodder powder is not added, and matsutake mushroom powder of equal quality to dodder powder is used instead, and the remaining operating steps and parameters are the same.

[0036] Comparative Example 3 Compared with Example 2, the difference is that no mannanase is added in step S3 of Comparative Example 3, and the remaining operation steps and parameters are the same.

[0037] Comparative Example 4 Compared with Example 2, the difference is that in step S3 of Comparative Example 4, no Lactobacillus acidophilus fermentation seed liquid is added, and the remaining operating steps and parameters are the same.

[0038] Comparative Example 5 Compared with Example 2, the difference is that in step S3 of Comparative Example 5, no thermophilic Streptococcus fermentation seed liquid is added, and the remaining operating steps and parameters are the same.

[0039] Embodiment 4-9 A method for preparing a skin-cleansing composition comprises the following steps: Cetyl ethylhexanoate, PEG-20 glyceryl triisostearate and ethanol are mixed at 32.5°C, and the remaining raw materials are added and mixed and stirred to obtain a finished skin cleansing composition.

[0040] The composition of each raw material and the weight parts of the raw materials in Examples 4-9 are shown in Table 1: Table 1 Example 10 The finished skin cleansing composition prepared in Example 9 was added to the cleansing cream at a mass percentage of 3%.

[0041] Embodiment 11 The finished skin cleansing composition prepared in Example 8 was added to the facial cleanser at a mass percentage of 5%.

[0042] Example 12 The finished skin cleansing composition prepared in Example 7 was added to the cleansing cream at a mass percentage of 4%.

[0043] Comparative Examples 6-10 Compared with Example 9, the difference between Comparative Examples 6-10 is that the plant fermentation liquid prepared in Comparative Examples 1-5 is used to replace the plant fermentation liquid prepared in Example 2 of Example 9, and the other operating steps and parameters are the same.

[0044] Comparative Example 11 Compared with Example 9, the difference is that in Comparative Example 11, cetyl ethylhexanoate is not added, and PEG-20 glyceryl triisostearate with half the weight of cetyl ethylhexanoate and soapberry seed oil with half the weight of cetyl ethylhexanoate are used instead, and the other operating steps and parameters are the same.

[0045] Comparative Example 12 Compared with Example 9, the difference is that in Comparative Example 12, PEG-20 glyceryl triisostearate is not added, and cetyl ethylhexanoate with half the weight of PEG-20 glyceryl triisostearate and soapberry seed oil with half the weight of PEG-20 glyceryl triisostearate are used instead, and the other operating steps and parameters are the same.

[0046] Comparative Example 13 Compared with Example 9, the difference is that in Comparative Example 13, no soapberry seed oil is added, and cetyl ethylhexanoate with half the weight of soapberry seed oil and PEG-20 glyceryl triisostearate with half the weight of soapberry seed oil are used instead, and the remaining operating steps and parameters are the same.

[0047] Comparative Example 14 Compared with Example 9, the difference is that in Comparative Example 14, no Artemisia selengensis extract is added, and half the weight of Artemisia selengensis extract is used instead of soapberry seed oil and half the weight of silk amino acids. The remaining operating steps and parameters are the same.

[0048] Comparative Example 15 Compared with Example 9, the difference is that in Comparative Example 15, no soapberry seed oil is added, and Artemisia selengensis extract with half the weight of soapberry seed oil and silk amino acids with half the weight of soapberry seed oil are used instead, and the remaining operating steps and parameters are the same.

[0049] Comparative Example 16 Compared with Example 9, the difference is that in Comparative Example 16, no silk amino acid is added, and half the weight of silk amino acid is used instead of Artemisia selengensis extract and half the weight of Sapindus mukorossi seed oil, and the other operating steps and parameters are the same.

[0050] Test Example 1 The tyrosinase inhibition ability of the finished skin cleansing compositions prepared in Examples 6-9 and Comparative Examples 6-10 was tested by a tyrosinase inhibition experiment, thereby reflecting the whitening effect.

[0051] Solution preparation: Prepare an enzyme solution with a concentration of 5 U / L, dispense into 1.5mL EP tubes, and store in liquid nitrogen. Before use, dilute with PBS to prepare a 0.5 U / L tyrosinase solution, and prepare it before use. Accurately weigh L-tyrosine and L-dopa, add an appropriate amount of PBS solution, and dissolve them by ultrasound at room temperature for 30 minutes to obtain a substrate solution with a concentration of 3.0mmol / L; Kojic acid (positive control) solution Accurately weigh 1g of kojic acid, dissolve it in DMSO, add an appropriate amount of PBS solution to dilute it, and the final DMSO content is 0.5%; Weigh 1g of the finished skin-cleansing composition obtained in Examples 6-9 and Comparative Examples 6-10 respectively, dissolve it in DMSO, add an appropriate amount of PBS solution to dilute it, and the final DMSO content is 0.5%.

[0052] Determination of tyrosinase activity inhibition rate: 40 μL of sample, 10 μL of PBS, and 10 μL of enzyme solution were mixed in sequence, incubated at 37°C for 10 min, 50 μL of substrate solution was added, and incubated for 25 min. The absorbance at 475 nm was detected on a multifunctional microplate reader, and the inhibition rate was calculated based on the absorbance value. The formula is as follows: Inhibition rate (%) = [blank - (assay - positive control)] / blank x 100%; The final results are shown in Table 2.

[0053] Table 2 It can be seen from Table 2 that the finished composition prepared by the present invention has an excellent tyrosinase activity inhibition rate, that is, the finished composition prepared by the present invention has an excellent whitening effect.

[0054] Test Example 2 Skin cleansing effect test: Number of subjects: 24 people / group, male and female in each group are randomized and all meet the subject criteria; Age: 22-45 years old; Test sample: finished skin cleansing composition obtained in Examples 6-9 and Comparative Examples 11-13.

[0055] Test method: Clean and dry the normal skin on the curved side of the forearm of the subject, apply the same commercially available foundation liquid on the forearm at a rate of 1.25 mg / cm 2 , dry for 30 minutes, apply 0.1g of the test sample to the parts with foundation, rub and rub in circles for 25 seconds, then rinse with water and wipe dry. Test the skin brightness value (L) of the test area before makeup, after makeup, and after skin cleansing to calculate the skin cleansing ability. The calculation formula is as follows: Formula: S=(L 净肤后 -L 上妆后 ) / (L 上妆前 -L 上妆后 ); Among them, S: skin cleansing ability, L: brightness after washing; The final result is the average value of each group. The specific test results are shown in Table 3.

[0056] Table 3 As can be seen from Table 3, the finished composition of the present invention has an outstanding skin cleansing ability.

[0057] Test Example 3 Moisturizing test: Male and female subjects aged 25-40 were randomly selected and divided into 7 groups, each with 12 subjects, half male and half female. Each group of subjects used a cleansing cream product prepared by adding the finished compositions prepared in Examples 6-9 and Comparative Examples 14-16 to the same cleansing cream base (the amount of the composition added to the cleansing cream was 3%).

[0058] The method of use is to evenly apply 1mL of the finished cleansing cream to the cheek area of ​​the face and rub it in circles for 25 seconds, then rinse with water and wipe dry. The Corneomenter CM 825 skin moisture content tester is used to measure the skin moisture content (MMV) of the subjects before use and 1h and 2h after use. The skin moisture content growth rate is calculated according to the following formula and the average value is recorded: Moisture content growth rate (%) = (MMV t -MMV0) / MMV0×100%; Where MMV0 is the skin moisture content before use, MMV t The moisture content of the skin during the t period after use; The results are shown in Table 4.

[0059] Table 4 It can be seen from Table 3 that the finished composition prepared by the present invention has an outstanding moisturizing effect.

[0060] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0061] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A skin cleansing composition, characterized in that: The skin cleansing composition specifically comprises the following raw materials in parts by weight: 4-6 parts by weight of plant fermentation liquid, 7-10 parts by weight of Artemisia selengensis extract, 15-22 parts by weight of silk amino acids, 44-52 parts by weight of cetyl ethylhexanoate, 20-26 parts by weight of PEG-20 glyceryl triisostearate, and 50-60 parts by weight of solvent; The method for preparing the plant fermentation liquid comprises the following steps: S1, pre-treating part of the raw materials to obtain a mixed solution A; S2, activating and culturing the strains to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid; S3, adjusting the pH of the mixed solution A, sterilizing under controlled temperature, cooling, adding mannanase to perform temperature-controlled enzymolysis, inactivating the enzyme after the enzymolysis is completed to obtain a mixed solution B, cooling, adding Lactobacillus acidophilus fermentation seed solution and thermophilic Streptococcus fermentation seed solution to perform temperature-controlled static fermentation, sterilizing under controlled temperature after the fermentation is completed, centrifuging under controlled speed, taking the supernatant and filtering it through a 0.22 μm membrane to obtain a plant fermentation liquid.

2. The skin cleansing composition according to claim 1, characterized in that The step S1 specifically comprises: air-drying, crushing and sieving Tricholoma matsutake and Cuscuta chinensis to obtain Tricholoma matsutake powder and Cuscuta chinensis powder; uniformly mixing the Tricholoma matsutake powder, Cuscuta chinensis powder and deionized water; and sterilizing under temperature control to obtain a mixed solution A for standby use.

3. The skin cleansing composition according to claim 2, characterized in that The mass ratio of the Tricholoma matsutake powder to the Cuscuta chinensis powder is 2-3:1.5-3; and the added amount of the deionized water is 100-110 times the total mass of the Tricholoma matsutake powder and the Cuscuta chinensis powder.

4. The skin cleansing composition according to claim 1, characterized in that The step S2 specifically comprises: inoculating Lactobacillus acidophilus and Streptococcus thermophilus into MRS liquid culture medium respectively, controlling the temperature once to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus, inoculating the activated Lactobacillus acidophilus and activated Streptococcus thermophilus into MRS liquid culture medium at an inoculum amount of 3-5% by volume, controlling the temperature twice to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid.

5. The skin cleansing composition according to claim 4, characterized in that The first temperature-controlled culture is: temperature-controlled at 37-42°C for 32-40 hours; the second temperature-controlled culture is: temperature-controlled at 37-42°C for 32-40 hours; the second temperature-controlled culture is: temperature-controlled at 37-42°C for 32-40 hours until the concentration of Lactobacillus acidophilus fermentation seed liquid is 2-2.1×10 8 CFU / mL, the concentration of thermophilic Streptococcus fermentation seed liquid is 1.8-2×10 8 CFU / mL.

6. The skin cleansing composition according to claim 1, characterized in that In step S3, the mass ratio of the mixed solution A to the mannanase is 1:0.002-0.003; the mass ratio of the mixed solution B, the fermented seed solution of Lactobacillus acidophilus and the fermented seed solution of Streptococcus thermophilus is 1:0.003-0.005:0.002-0.

003.

7. The skin cleansing composition according to claim 6, characterized in that The enzymatic activity of the mannanase includes 1×10 4 U / g.

8. The skin cleansing composition according to claim 1, characterized in that The solvent includes ethanol.

9. The skin cleansing composition according to claim 1, characterized in that The skin cleansing composition further comprises 1-1.6 parts by weight of soapberry seed oil.

10. Use of the skin cleansing composition according to claim 9, characterized in that: The skin-cleansing composition is added to skin care products at a mass percentage of 3-5%.

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