Sample analyzer and control method of ultrasonic device thereof

Abstract

The invention is suitable for the field of in vitro diagnostic equipment, and discloses a sample analyzer and a control method of an ultrasonic device in the sample analyzer. The sample analyzer comprises a sample distribution device, a reagent distribution device, an ultrasonic device, a determination device and a control device, the control device is configured to control the ultrasonic device to execute the following calibration operations: controlling the ultrasonic device to transmit ultrasonic waves to the first reference medium, and obtaining a first working parameter of the ultrasonic device; controlling the ultrasonic device to transmit ultrasonic waves to a second reference medium, and acquiring a second working parameter of the ultrasonic device; obtaining a calibration coefficient of the ultrasonic device at least according to the first working parameter and the second working parameter; and the control device is further configured to control the ultrasonic device to execute the following ultrasonic mixing operation: determining a target parameter according to the calibration coefficient, and driving the ultrasonic device to perform ultrasonic mixing on the target liquid according to the target parameter. According to the invention, on-machine automatic calibration of the ultrasonic device is realized, and the calibration cost of the ultrasonic device is reduced.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic equipment, and in particular to a sample analyzer and a control method for an ultrasonic device in the sample analyzer. Background Art

[0002] A sample analyzer provided by the related art is provided with an ultrasonic device for ultrasonically working on liquids. After working for a long time, the ultrasonic device will mutate, and the electromechanical conversion efficiency of the mutated ultrasonic device will change, resulting in the failure to achieve the expected ultrasonic vibration effect when the original driving parameters are used to drive the ultrasonic device. Therefore, in specific applications, the ultrasonic device needs to be calibrated and replaced regularly.

[0003] In the related art, the commonly used calibration methods are: 1) Using a laser Doppler vibrometer to measure the output amplitude or vibration velocity of the ultrasonic device under a given series of driving parameters, and using the interpolation method to obtain the driving voltage or driving current corresponding to the required output amplitude. 2) Using a microphone or purified hydrophone to measure the sound pressure and / or sound intensity generated by the ultrasonic device at a certain distance in the medium under different driving parameters, and using the interpolation method to obtain the driving voltage and current corresponding to the required output amplitude. These two calibration methods require the introduction of relevant measuring equipment for each calibration. Therefore, on the one hand, the calibration cost is relatively high, and on the other hand, the calibration operation of the ultrasonic device is relatively cumbersome, requiring the operator to occupy more time and energy. Summary of the invention

[0004] The first object of the present invention is to provide a sample analyzer, which aims to solve the technical problems of high cost and complicated operation of calibration of ultrasonic devices in sample analyzers in the related art.

[0005] To achieve the above object, the present invention provides a solution: a sample analyzer, comprising:

[0006] A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and distribute at least a portion of the drawn sample to a reaction container;

[0007] A reagent dispensing device, the reagent dispensing device is used to suck the reagent from the reagent container and distribute at least part of the sucked reagent to the reaction container;

[0008] An ultrasonic device, the ultrasonic device is used to ultrasonically mix a target liquid, the target liquid being one of the sample, the reagent, and a mixed liquid containing at least the sample and the reagent;

[0009] a measuring device for measuring a reaction solution made of at least a mixed solution;

[0010] A control device, wherein the control device is configured to control the ultrasonic device to perform the following calibration operations: control the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtain a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtain a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; and obtain a calibration coefficient of the ultrasonic device based on at least the first working parameter and the second working parameter;

[0011] The control device is further configured to control the ultrasonic device to perform the following ultrasonic mixing operation: determining a target parameter according to the calibration coefficient, and driving the ultrasonic device to operate with the target parameter to ultrasonically mix the target liquid;

[0012] The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

[0013] As an embodiment, the obtaining of the ultrasonic device transmitting ultrasonic waves to the first reference medium includes: obtaining a first operating voltage and a first operating current when the ultrasonic device transmits ultrasonic waves to the first reference medium and satisfies a first preset condition;

[0014] The obtaining of the second operating parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium comprises: obtaining a second operating voltage and a second operating current when the ultrasonic device satisfies the first preset condition in the process of transmitting ultrasonic waves to the second reference medium;

[0015] Wherein, the first preset condition includes: the driving frequency of the ultrasonic device is within a first preset range of the resonant frequency, and / or the phase difference between the working voltage and the working current of the ultrasonic device is within a second preset range.

[0016] As an embodiment, the first reference medium is air; and / or,

[0017] The second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components.

[0018] As an embodiment, the control device is further configured to: before controlling the ultrasonic device to perform the calibration operation, obtain a first standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium, and / or obtain a second standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium;

[0019] The obtaining of the calibration coefficient of the ultrasonic device at least based on the first working parameter and the second working parameter includes: obtaining the calibration coefficient of the ultrasonic device based on the first working parameter, the second working parameter, and the first standard parameter and / or the second standard parameter.

[0020] As an embodiment, the first reference medium is air, and the control device is configured to: before controlling the ultrasonic device to perform the calibration operation, obtain a second standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; obtaining the calibration coefficient of the ultrasonic device based on at least the first working parameter and the second working parameter includes: obtaining the calibration coefficient of the ultrasonic device based on the first working parameter, the second working parameter and the second standard parameter; and / or,

[0021] The second standard parameter is the mechanical load impedance generated by the calibrated ultrasonic device transmitting ultrasonic waves to the second reference medium.

[0022] As an implementation manner, the second standard parameter is a pre-stored value pre-stored in the sample analyzer; or,

[0023] The control device is also configured to determine the second standard parameter in the following manner: obtaining a third working parameter of the calibrated ultrasonic device transmitting ultrasonic waves under no-load conditions; controlling the calibrated ultrasonic device to transmit ultrasonic waves to the second reference medium, and obtaining a fourth working parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; and obtaining the second standard parameter based on the third working parameter and the fourth working parameter.

[0024] As an embodiment, the ultrasonic device is used to ultrasonically mix the target liquid located at the ultrasonic mixing position and in the reaction container;

[0025] The second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components;

[0026] The controlling the ultrasonic device to transmit ultrasonic waves to the second reference medium and obtaining the second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium comprises: controlling the liquid transfer device to dispense the second reference medium into an empty reaction container, controlling the reaction container moving device to move the reaction container loaded with the second reference medium to the ultrasonic mixing position, controlling the ultrasonic device to perform ultrasonic work on the second reference medium located at the ultrasonic mixing position and in the reaction container, and obtaining the second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium;

[0027] The pipetting device is: the reagent dispensing device, or the sample dispensing device, or a device independent of the reagent dispensing device and the sample dispensing device.

[0028] As an embodiment, the calibration coefficient includes the electromechanical conversion efficiency of the ultrasonic device; determining the target parameter according to the calibration coefficient includes: determining the target parameter according to the calibration coefficient and a preset vibration speed of the ultrasonic device; and / or,

[0029] The ultrasonic device includes an ultrasonic transducer, an ultrasonic vibrator and a driving mechanism. The ultrasonic vibrator is used to ultrasonically mix the target liquid under the drive of the ultrasonic transducer, and the driving mechanism is used to drive the ultrasonic vibrator to move. The control device is also configured to: before driving the ultrasonic device to work with the target parameters, control the driving mechanism to drive the ultrasonic vibrator to insert into the target liquid.

[0030] As an embodiment, the calibration coefficient of the ultrasonic device is obtained at least based on the first working parameter and the second working parameter, including: obtaining a first impedance value formed by the first reference medium to the ultrasonic device according to the first working parameter, obtaining a second impedance value formed by the second reference medium to the ultrasonic device according to the second working parameter, and obtaining the calibration coefficient of the ultrasonic device at least based on the first impedance value and the second impedance value.

[0031] As an embodiment, the sample analyzer pre-stores at least one of the following second preset conditions: the duration for which the ultrasonic device continuously performs the ultrasonic mixing operation reaches a first preset duration, the duration for which the ultrasonic device cumulatively performs the ultrasonic mixing operation since the last calibration operation reaches a second preset duration, the number of times the ultrasonic device continuously performs the ultrasonic mixing operation reaches a first preset number of times, the number of times the ultrasonic device cumulatively performs the ultrasonic mixing operation since the last calibration operation reaches a second preset number of times, the first preset time point after the sample analyzer is turned on is reached, the second preset time point before the sample analyzer is turned off is reached, and the third preset time length is reached since the last calibration operation is performed; the ultrasonic device is replaced; the third preset time point of a preset period is reached, and the preset period is every day or every two days or every three days or every four days or every five days or every week or every two weeks or every half a month or every month or every two months or every three months or every six months;

[0032] The control device is further configured to: when any one of the second preset conditions is met, control the ultrasonic device to perform the calibration operation.

[0033] As an embodiment, the control device is further configured to: determine the variation state of the ultrasonic device at least based on the first working parameter and the second working parameter.

[0034] As an embodiment, the control device is further configured to: output a warning signal when it is determined that the variation state of the ultrasonic device meets a third preset condition.

[0035] A second object of the present invention is to provide a sample analyzer, the sample analyzer comprising:

[0036] A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and distribute at least a portion of the drawn sample to a reaction container;

[0037] A reagent dispensing device, the reagent dispensing device is used to suck the reagent from the reagent container and distribute at least part of the sucked reagent to the reaction container;

[0038] An ultrasonic device, the ultrasonic device is used to perform ultrasonic work on a target liquid, the target liquid is one of the sample, the reagent, a cleaning solution, and a mixed solution containing at least the sample and the reagent;

[0039] a measuring device for measuring a reaction solution made of at least a mixed solution;

[0040] A control device, wherein the control device is configured to: control the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtain a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtain a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; and determine a variation state of the ultrasonic device based on at least the first working parameter and the second working parameter;

[0041] The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

[0042] As an embodiment, the control device is further configured to: output a warning signal when it is determined that the variation state of the ultrasonic device meets a third preset condition; and / or,

[0043] The first reference medium is air, and the second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components.

[0044] As an implementation manner, the obtaining of the first operating parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium includes: obtaining a first operating voltage and a first operating current when the ultrasonic device satisfies a first preset condition in the process of transmitting ultrasonic waves to the first reference medium;

[0045] The obtaining of the second operating parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium comprises: obtaining a second operating voltage and a second operating current when the ultrasonic device satisfies the first preset condition in the process of transmitting ultrasonic waves to the second reference medium;

[0046] Wherein, the first preset condition includes: the driving frequency of the ultrasonic device is within a first preset range of the resonant frequency, and / or the phase difference between the working voltage and the working current of the ultrasonic device is within a second preset range.

[0047] As an embodiment, the control device is further configured to: before controlling the ultrasonic device to perform the calibration operation, obtain a first standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium, and / or obtain a second standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium;

[0048] Determining the variation state of the ultrasonic device at least based on the first working parameter and the second working parameter includes: determining the variation state of the ultrasonic device based on the first working parameter, the second working parameter, and the first standard parameter and / or the second standard parameter.

[0049] A third object of the present invention is to provide a method for controlling an ultrasonic device in a sample analyzer, the method comprising the following steps:

[0050] Controlling the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium;

[0051] Controlling the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium;

[0052] Obtaining a calibration coefficient of the ultrasonic device based at least on the first operating parameter and the second operating parameter;

[0053] Determining a target parameter according to the calibration coefficient, and driving the ultrasonic device to operate with the target parameter to ultrasonically mix the target liquid;

[0054] The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

[0055] A fourth object of the present invention is to provide a control method for an ultrasonic device in a sample analyzer, the control method comprising the following steps:

[0056] Controlling the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium;

[0057] Controlling the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium;

[0058] determining a variation state of the ultrasonic device at least according to the first operating parameter and the second operating parameter;

[0059] The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

[0060] The sample analyzer and the control method of the ultrasonic device in the sample analyzer provided by the present invention obtain the calibration coefficient of the ultrasonic device through the first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium and the second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; then, according to the calibration coefficient, the target parameter required for the ultrasonic device to ultrasonically mix the target liquid is determined, and the ultrasonic device is driven to work with the target parameter, thereby realizing the automatic calibration of the ultrasonic device on the machine. Since the calibration of the ultrasonic device in the present invention is to realize the on-machine calibration through program control using the inherent device of the sample analyzer, there is no need for the operator to introduce other measuring equipment or sensors, so the calibration cost of the ultrasonic device is effectively reduced. This calibration method has significant advantages in cost, integration space, and integration ease of use. In addition, the operator does not need to perform complex operations during the calibration process, which avoids the occupation of the operator's time and energy. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0062] Figure 1 is a schematic diagram of the composition of a sample analyzer provided by an embodiment of the present invention;

[0063] Figure 2 is a schematic diagram of the structure of a sample analyzer provided by an embodiment of the present invention;

[0064] Figure 3 is a schematic diagram of ultrasonic mixing of a target liquid by an ultrasonic device provided in an embodiment of the present invention;

[0065] Figure 4 is a schematic diagram of an electromechanical equivalent model of an ultrasonic device provided in an embodiment of the present invention;

[0066] Figure 5 It is a schematic diagram of the composition of the control device provided in an embodiment of the present invention.

[0067] Explanation of the accompanying drawings: 10. Sample analyzer; 100. Ultrasonic device; 110. Ultrasonic transducer; 111. Piezoelectric ceramic component; 120. Ultrasonic vibrator; 200. Control device; 210. Ultrasonic control board; 220. Main control board; 300. Sample dispensing device; 400. Reagent dispensing device; 500. Measurement device; 600. Reagent loading device; 700. Incubation device; 800. Transfer device; 900. Reaction container providing device; 101. Sample management device; 102. Sample transport device; 103. Magnetic separation device; 20. Target liquid; 30. Reaction container. DETAILED DESCRIPTION

[0068] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0069] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0070] It should also be noted that when an element is referred to as being "fixed on" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element at the same time. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or may be indirectly connected to the other element through an intermediate element.

[0071] In addition, the descriptions of "first", "second", etc. in the present invention are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0072] Embodiment 1:

[0073] like Figures 1 to 5 As shown, the sample analyzer 10 provided in the first embodiment of the present invention includes an ultrasonic device 100, and the ultrasonic device 100 is used to ultrasonically mix the target liquid 20, and the target liquid 20 is one of a sample, a reagent, and a mixed liquid containing at least a sample and a reagent. The ultrasonic device 100 achieves the effect of mixing the target liquid 20 by transmitting ultrasonic waves to the target liquid 20. The use of the ultrasonic device 100 to mix the target liquid 20 has the characteristic of high mixing efficiency, which is conducive to improving the sample measurement efficiency of the sample analyzer 10.

[0074] As an embodiment, the sample analyzer 10 further includes a control device 200, which is configured to control the ultrasonic device 100 to perform the following calibration operations: control the ultrasonic device 100 to transmit ultrasonic waves to a first reference medium, and obtain a first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device 100 to transmit ultrasonic waves to a second reference medium, and obtain a second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; obtain a calibration coefficient of the ultrasonic device 100 based on at least the first working parameter and the second working parameter. The first reference medium and the second reference medium are two different media that are different from the target liquid 20. The ultrasonic device 100 transmits ultrasonic waves to the first reference medium, that is, the ultrasonic device 100 performs ultrasonic work under the working condition of the first reference medium. The ultrasonic device 100 transmits ultrasonic waves to the second reference medium, that is, the ultrasonic device 100 performs ultrasonic work under the working condition of the second reference medium. This embodiment obtains the working parameters of the ultrasonic device 100 under different working conditions and calculates the calibration coefficient of the ultrasonic device 100, so that the target parameters of the ultrasonic device 100 can be calibrated according to the calibration coefficient, thereby realizing the calibration of the ultrasonic device 100. Since the calibration of the ultrasonic device 100 is realized on-machine calibration using the inherent device of the sample analyzer 10, no other measuring equipment needs to be introduced, so the calibration cost of the ultrasonic device 100 is effectively reduced. This calibration method has significant advantages in cost, integration space, and ease of integration. In addition, during the calibration process, the sample analyzer 10 is automatically controlled and executed according to the set program, and the operator does not need to manually connect any auxiliary measuring equipment, thereby avoiding the occupation of the operator's time and energy during the calibration process.

[0075] As an embodiment, the first reference medium and the second reference medium are two different media different from the target liquid 20, including: the first reference medium and the second reference medium are two different media whose physical properties and / or components are different from the target liquid 20. The difference in physical properties of the two media includes at least one of the following situations: different density, different sound propagation speed, and different viscosity. The difference in composition of the two media means that the components of the two media are different.

[0076] As an embodiment, the first reference medium is air. The ultrasonic device 100 transmits ultrasonic waves to the air, that is, the ultrasonic device 100 works under no-load conditions. In this embodiment, the first reference medium is set as air, which can save the preparation time of the first reference medium and the supply device of the first reference medium, thereby helping to reduce the calibration time and calibration cost of the ultrasonic device 100. Of course, in specific applications, the setting method of the first reference medium is not limited to this. For example, as an alternative embodiment, the first reference medium can also be other reference liquids that are different from the second reference medium and the target liquid 20.

[0077] As an embodiment, the second reference medium is purified water, and the ultrasonic device 100 transmits ultrasonic waves to the purified water, that is, the ultrasonic device 100 transmits ultrasonic waves to the purified water in the container. In this embodiment, the second reference medium is set as purified water. Since purified water is easy to obtain and has low cost, it is beneficial to reduce the calibration cost of the ultrasonic device 100. Of course, in specific applications, the setting method of the second reference medium is not limited to this. As an alternative embodiment, the second reference medium can also be other reference liquids that are different from purified water and have known physical properties and / or known components, such as diluents or other standard liquids.

[0078] As an implementation mode, the ultrasonic device 100 transmits ultrasonic waves to the first reference medium, that is, the ultrasonic device 100 performs ultrasonic work under the working condition of the first reference medium. When the first reference medium is a liquid, the position where the ultrasonic device 100 transmits ultrasonic waves to the first reference medium and the position where the ultrasonic device 100 performs ultrasonic mixing on the target liquid can be the same position or different positions; the container for loading the first reference medium and the container for loading the target liquid can be containers of the same size and shape, or containers of different sizes and / or shapes.

[0079] As an implementation mode, the ultrasonic device 100 transmits ultrasonic waves to the second reference medium, that is, the ultrasonic device 100 performs ultrasonic work under the working condition of the second reference medium. When the second reference medium is a liquid, the position where the ultrasonic device 100 transmits ultrasonic waves to the second reference medium and the position where the ultrasonic device 100 performs ultrasonic mixing on the target liquid can be the same position or different positions; the container for loading the second reference medium and the container for loading the target liquid can be containers of the same size and shape, or containers of different sizes and / or shapes.

[0080] As an embodiment, the control device 200 is further configured to control the ultrasonic device 100 to perform the following ultrasonic mixing operation: according to the calibration coefficient, determine the target parameter, drive the ultrasonic device 100 to work with the target parameter, and drive the ultrasonic device 100 to ultrasonically mix the target liquid 20. In this embodiment, according to the calibration coefficient, the target parameter of the ultrasonic device 100 is adjusted to avoid the undesirable phenomenon that the ultrasonic mixing effect is reduced due to the change of the electromechanical conversion efficiency of the ultrasonic device 100, thereby helping to avoid the influence of the ultrasonic device 100 on the operation of the sample analyzer 10, for example, avoiding the undesirable phenomenon that the accuracy of the sample measurement result is reduced due to insufficient ultrasonic mixing.

[0081] As an embodiment, the above-mentioned acquisition of the first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium includes: acquiring the first working parameter of the ultrasonic device 100 when the first preset condition is met in the process of transmitting ultrasonic waves to the first reference medium. The above-mentioned acquisition of the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium includes: acquiring the second working parameter of the ultrasonic device 100 when the first preset condition is met in the process of transmitting ultrasonic waves to the second reference medium. The first preset condition includes: the driving frequency of the ultrasonic device 100 is within the first preset range of the resonant frequency, and / or, the phase difference between the working voltage and the working current of the ultrasonic device 100 is within the second preset range. The first preset range is that the driving frequency of the ultrasonic device 100 is equal to the resonant frequency of the ultrasonic device 100 or is within the preset range close to the resonant frequency. The second preset range is that the phase difference between the working voltage and the working current of the ultrasonic device 100 is zero or within the preset range close to zero, which can also indicate that the driving frequency of the ultrasonic device 100 is within the first preset range of the resonant frequency. When the driving frequency input to the ultrasonic device 100 is the same as the resonance frequency of the dynamic branch of the ultrasonic device 100, the no-load dynamic branch of the ultrasonic device 100 is purely resistive, and at this time, the impedance of the ultrasonic device 100 is the smallest, and the electromechanical conversion efficiency of the ultrasonic device 100 is the highest. In this embodiment, by collecting the operating parameters when the driving frequency of the ultrasonic device 100 is approximately equal to the resonance frequency of the ultrasonic device 100 under different working conditions, as the first operating parameter and the second operating parameter, and then using the first operating parameter and the second operating parameter to calculate the calibration coefficient of the ultrasonic device 100, it is helpful to reduce the interference of other factors to ensure the accuracy of the calibration coefficient.

[0082] As an embodiment, the first working parameter includes a first working voltage and a first working current. The above-mentioned acquisition of the first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium includes: acquiring the first working voltage and the first working current when the ultrasonic device 100 satisfies the first preset condition in the process of transmitting ultrasonic waves to the first reference medium. The second working parameter includes a second working voltage and a second working current. The above-mentioned acquisition of the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium includes: acquiring the second working voltage and the second working current when the ultrasonic device 100 satisfies the first preset condition in the process of transmitting ultrasonic waves to the second reference medium. The first preset condition is that the phase difference between the working voltage and the working current of the ultrasonic device 100 (the phase difference between the first working voltage and the first working current under the working condition of the first reference medium, and the phase difference between the second working voltage and the second working current under the working condition of the second reference medium) is within a second preset range. In this embodiment, whether the driving frequency of the ultrasonic device 100 meets the second preset range of the resonant frequency is determined by determining whether the phase difference between the working voltage and the working current of the ultrasonic device 100 is zero or within a preset range near zero, and the first working voltage and the first working current when the driving frequency is within the second preset range of the resonant frequency are used as the first working parameters, and the second working voltage and the second working current when the driving frequency is within the second preset range of the resonant frequency are used as the second working parameters. This method is easy to implement.

[0083] As an embodiment, the control device 200 is further configured to: before controlling the ultrasonic device 100 to perform the calibration operation, obtain the first standard parameter of the calibrated ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium, and / or obtain the second standard parameter of the calibrated ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium. The calibration coefficient of the ultrasonic device 100 is obtained at least according to the first working parameter and the second working parameter, including: obtaining the calibration coefficient of the ultrasonic device 100 according to the first working parameter, the second working parameter, and the first standard parameter and / or the second standard parameter. The calibrated ultrasonic device 100 is also called the standard ultrasonic device 100, that is, an ultrasonic device 100 with a known calibration coefficient. The first standard parameter and the second standard parameter are obtained before the ultrasonic device 100 performs the following calibration operation. The ultrasonic device 100 for performing the calibration operation and the ultrasonic device 100 for performing the ultrasonic mixing operation are the same device at different time periods. Here, the calibrated ultrasonic device 100 and the ultrasonic device 100 for performing the ultrasonic mixing operation can be the same device at different time periods, or can be two different devices at different time periods. In this embodiment, before the ultrasonic device 100 performs a calibration operation, standard parameters of the ultrasonic device 100 with known calibration coefficients under a first reference medium condition and / or standard parameters under a second reference medium condition are first obtained, and during the calibration operation, the calibration coefficient of the ultrasonic device 100 is calculated using the standard parameters and the first operating parameters and the second operating parameters to eliminate the influence of mechanical load impedance under different operating conditions and improve the accuracy of the calibration.

[0084] As an implementation method, the standard parameter is the mechanical load impedance generated by the ultrasonic device 100 transmitting ultrasonic waves to the reference medium. Specifically, the second standard parameter is the mechanical load impedance generated by the calibrated ultrasonic device 100 transmitting ultrasonic waves to the second reference medium. The size of the equivalent electrical load impedance generated by the ultrasonic device 100 acting on the medium depends on the mechanical load impedance and the electromechanical conversion efficiency of the ultrasonic device 100. The mechanical load impedance depends on the properties of the object of action (density, viscosity, sound speed, etc.), the boundary conditions of the sound field (container shape, container material, liquid volume, relative position, etc.); for the same container, the same liquid (such as purified water), and the ultrasonic device 100 outputting ultrasonic vibrations at the same position, the mechanical load impedance received can be considered to be the same, thereby constructing a fixed mechanical load condition. In this implementation method, before the ultrasonic device 100 performs the calibration operation, the mechanical load impedance under the working condition required for calibration is first obtained, so that the mechanical load impedance under the working condition can be directly called for calculation during the calibration operation. Of course, in specific applications, the setting method of the second standard parameter is not limited to this, for example, it can also be other parameters related to the mechanical load impedance or parameters used to characterize the mechanical load impedance.

[0085] As an embodiment, the first reference medium is air, and the control device 200 is configured to: before controlling the ultrasonic device 100 to perform a calibration operation, obtain the second standard parameter of the calibrated ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; the calibration coefficient of the ultrasonic device 100 is obtained at least according to the first working parameter and the second working parameter, including: obtaining the calibration coefficient of the ultrasonic device 100 according to the first working parameter, the second working parameter and the second standard parameter. Since the first reference medium is air, the ultrasonic operation of the ultrasonic device 100 under the working condition of the first reference medium is no-load ultrasonic operation. The mechanical load impedance generated by the ultrasonic device 100 transmitting ultrasonic waves to the first reference medium can be ignored. Therefore, before the ultrasonic device 100 performs a calibration operation, it is only necessary to first obtain the mechanical load impedance of the ultrasonic device 100 under the working condition of the second reference medium. Of course, in specific applications, if the first reference medium is not air but other liquids, the first standard parameter corresponds to the mechanical load impedance generated by the ultrasonic device 100 transmitting ultrasonic waves to the first reference medium. In this way, before the ultrasonic device 100 performs a calibration operation, in addition to obtaining the mechanical load impedance of the ultrasonic device 100 under the second reference medium working condition, it is also necessary to first obtain the mechanical load impedance of the ultrasonic device 100 under the first reference medium working condition.

[0086] As an embodiment, the control device 200 is further configured to determine the second standard parameter in the following manner: obtaining the third working parameter of the calibrated ultrasonic device 100 transmitting ultrasonic waves under no-load conditions; controlling the calibrated ultrasonic device 100 to transmit ultrasonic waves to the second reference medium, and obtaining the fourth working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; and obtaining the second standard parameter according to the third working parameter and the fourth working parameter. Under the driving action of the dynamic branch resonant frequency, the impedance of the static branch of the ultrasonic device 100 is so large that the influence of the static branch can be ignored, so that the working voltage and working current at both ends of the ultrasonic device 100 measured under no-load and fixed mechanical load conditions can be used to calculate the impedance under different working conditions. In a specific application, the third working parameter includes the third working voltage U3 and the third working current I3 of the standard ultrasonic device 100 (ultrasonic device 100 with a known calibration coefficient N0) under no-load conditions, and the no-load impedance R1 can be calculated according to the formula U3 / I3=R1. The fourth working parameter includes a fourth working voltage U4 and a fourth working current I4 of the standard ultrasonic device 100 under the second reference medium working condition, according to the formula U4 / I4=R1+N0 2Zs, the mechanical load impedance Zs generated by the ultrasonic device 100 transmitting ultrasonic waves to the second reference medium is calculated. In this embodiment, the second standard parameter is calculated by running a program after the sample analyzer 10 is installed in a specific application. Of course, in a specific application, as an alternative embodiment, the second standard parameter can also be a pre-stored value pre-stored in the sample analyzer 10, that is, the second standard parameter is pre-stored in the sample analyzer 10 before leaving the factory.

[0087] In the above embodiment, the calibration coefficient of the ultrasonic device 100 is calculated by the first operating voltage and the first operating current of the ultrasonic device 100 under the first reference medium working condition, and the second operating voltage, the first operating current and the mechanical load impedance of the ultrasonic device 100 under the second reference medium working condition. Of course, in specific applications, as an alternative implementation scheme, the first impedance value of the ultrasonic device 100 under the first reference medium working condition can be calculated based on the first working voltage and the first working current of the ultrasonic device 100 under the first reference medium working condition, and the second impedance value of the ultrasonic device 100 under the second reference medium working condition can be calculated based on the second working voltage and the first working current of the ultrasonic device 100 under the second reference medium working condition, and then the calibration coefficient of the ultrasonic device 100 can be calculated using the first impedance value, the second impedance value and the mechanical load impedance. That is, in this implementation scheme, the calibration coefficient of the ultrasonic device 100 is obtained at least based on the first working parameter and the second working parameter, including: obtaining the first impedance value formed by the first reference medium to the ultrasonic device 100 according to the first working parameter, obtaining the second impedance value formed by the second reference medium to the ultrasonic device 100 according to the second working parameter, and obtaining the calibration coefficient of the ultrasonic device 100 at least based on the first impedance value and the second impedance value.

[0088] As an embodiment, the calibration coefficient includes the electromechanical conversion efficiency of the ultrasonic device 100. The above-mentioned determination of the target parameter according to the calibration coefficient includes: determining the target parameter according to the calibration coefficient and the preset vibration velocity of the ultrasonic device 100. The preset vibration velocity is the vibration velocity required for the ultrasonic device 100 to ultrasonically work on the target liquid 20. The target parameter can be the target driving current or target driving voltage or target driving power of the ultrasonic device 100. According to the calibration operation, after the electromechanical conversion coefficient N and the no-load impedance R1 of the ultrasonic device 100 are obtained, the response characteristics of the ultrasonic device 100 can be known, that is, the calibration of the ultrasonic device 100 has been achieved, and according to the electromechanical conversion relationship V=NI, the target driving current I corresponding to the required vibration velocity V output by the ultrasonic device 100 can be calculated according to the actual use scenario, or the target driving voltage or target driving power of the ultrasonic device 100 can be calculated in combination with the relationship between current, voltage and power, or further parameters related to the vibration velocity V, such as amplitude, sound pressure, sound intensity, etc., can be determined in combination with the resonant frequency fs to reach the driving current I corresponding to the specified value.

[0089] Reference Figure 3 As shown, as an embodiment, the ultrasonic device 100 includes an ultrasonic transducer 110 and an ultrasonic vibrator 120, the ultrasonic vibrator 120 is connected to the ultrasonic transducer 110, and the ultrasonic vibrator 120 is used to ultrasonically mix the target liquid 20 under the drive of the ultrasonic transducer 110. The control device 200 is electrically connected to the ultrasonic transducer 110 to control the operation of the ultrasonic transducer 110. The ultrasonic transducer 110 is an energy conversion device, which is mainly used to convert the electrical power input by the control device 200 into mechanical power (i.e., ultrasonic waves) and then transmit it out.

[0090] As an implementation mode, the ultrasonic transducer 110 includes a piezoelectric ceramic component 111. The piezoelectric ceramic component 111 is a component made of an electronic ceramic material having piezoelectric and sensitive properties, and can realize mutual conversion between mechanical energy and electrical energy.

[0091] As an embodiment, the ultrasonic vibrator 120 is an ultrasonic vibrating rod for at least partially inserting into the target liquid 20 for ultrasonic mixing. The ultrasonic vibrator 120 transmits ultrasonic waves to the target liquid 20 in a contact manner, which helps to reduce the interference of other external components on the ultrasonic transmission. Of course, in specific applications, the arrangement of the ultrasonic vibrator 120 is not limited to this. For example, as an alternative embodiment, the ultrasonic vibrator 120 is a component for ultrasonically mixing the target liquid 20 in a non-contact manner, and its specific form includes but is not limited to the following situations: an ultrasonic mixing seat for placing a reaction container 30 loaded with the target liquid 20, a vibration rod for transmitting ultrasonic waves to the target liquid 20 from the side of the outside of the reaction container 30 loaded with the target liquid 20, and a vibration rod for inserting into the reaction container 30 loaded with the target liquid 20 but not into the target liquid 20.

[0092] As an implementation mode, the ultrasonic vibration rod is a horn. The end surface of the ultrasonic vibration rod at one end away from the ultrasonic transducer 110 is the main output surface of the ultrasonic vibration rod for transmitting ultrasonic waves outward.

[0093] As an embodiment, the ultrasonic device 100 further includes a driving mechanism, the ultrasonic transducer 110 and / or the ultrasonic vibrator 120 are connected to the driving mechanism, the driving mechanism is used to drive the ultrasonic transducer 110 and the ultrasonic vibrator 120 to move, and the control device 200 is further configured to: before driving the ultrasonic device 100 to work with the target parameters, control the driving mechanism to drive the ultrasonic vibrator 120 to insert the target liquid 20. The driving mechanism is used to drive the ultrasonic vibrator 120 to move in two-dimensional or three-dimensional space, so that the ultrasonic vibrator 120 moves to different positions, such as a standby position, an ultrasonic mixing position, a cleaning position, etc.

[0094] As an implementation mode, in the ultrasonic mixing situation, the ultrasonic device 100 is used to ultrasonically mix the target liquid 20 located at the ultrasonic mixing position and located in the reaction container 30. In the calibration operation situation, the ultrasonic device 100 is used to ultrasonically work the second reference medium located at the ultrasonic mixing position and located in the reaction container 30. In this implementation mode, the ultrasonic mixing position is reused as the ultrasonic location of the second reference medium in the calibration operation situation, so that there is no need to add an additional working position of the ultrasonic device 100 for the calibration operation, which helps to simplify the control complexity of the ultrasonic device 100.

[0095] It should be noted that, when the ultrasonic device 100 with the same size of ultrasonic vibrator 120 is used and measured at the same position of a container with the same size and filled with the same medium, the mechanical load impedance is the same.

[0096] In the above scheme, the fixed mechanical load condition required in the calibration operation is constructed at the ultrasonic mixing position. Of course, in specific applications, as an alternative implementation scheme, the fixed mechanical load condition can also be constructed in other ways, such as ultrasound acting on a specified object, or liquid in a sufficiently large container (the influence of the acoustic field boundary conditions can be ignored).

[0097] As an embodiment, the sample analyzer 10 pre-stores at least one of the following second preset conditions: the duration of the ultrasonic mixing operation continuously performed by the ultrasonic device 100 reaches a first preset duration, the cumulative duration of the ultrasonic mixing operation performed by the ultrasonic device 100 since the last calibration operation reaches a second preset duration, the number of times the ultrasonic device 100 continuously performs the ultrasonic mixing operation reaches a first preset number of times, the cumulative number of times the ultrasonic mixing operation performed by the ultrasonic device 100 since the last calibration operation reaches a second preset number of times, the first preset time point after the sample analyzer 10 is turned on is reached, the second preset time point before the sample analyzer 10 is turned off is reached, the third preset time length is reached since the last calibration operation is performed; the ultrasonic device 100 is replaced; the third preset time point of the preset cycle is reached. The control device 200 is also configured to: when any of the second preset conditions is met, control the ultrasonic device 100 to perform the calibration operation. Replacing the ultrasonic device 100 includes replacing the ultrasonic transducer 110 and / or the ultrasonic vibrator 120. In this embodiment, the calibration operation of the ultrasonic device 100 will not occupy the ultrasonic mixing cycle time of the ultrasonic device 100, and when the second preset condition is met, it will be automatically executed according to the set program, which is conducive to making the operator basically unaware of the calibration operation of the ultrasonic device 100, and greatly reducing the interference of the calibration operation of the ultrasonic device 100 on the operator.

[0098] As an implementation mode, the preset period is every day, every two days, every three days, every four days, every five days, every week, every two weeks, every half month, every month, every two months, every three months, or every six months. Of course, the setting method of the preset period is not limited thereto, and can be flexibly set according to specific actual conditions, such as the frequency of use and service life of the ultrasonic device 100.

[0099] As an embodiment, the control device 200 is further configured to: determine the variation state of the ultrasonic device 100 at least according to the first working parameter and the second working parameter. The variation state may include whether the ultrasonic device 100 varies. In this embodiment, according to the first working parameter under the first reference medium working condition and the second working parameter under the second reference medium working condition, in addition to obtaining the calibration coefficient, it can also be used to determine the variation state of the ultrasonic device 100, so that the operator can promptly know whether the ultrasonic device 100 varies.

[0100] As an embodiment, the control device 200 is further configured to: output a warning signal when it is determined that the variation state of the ultrasonic device 100 meets the third preset condition. The warning signal is mainly used to prompt the operator to replace the ultrasonic device 100 when the ultrasonic device 100 varies. The warning signal includes at least one of the following forms: a prompt on the display screen, a sound warning signal, and a light warning signal.

[0101] As an implementation mode, the third preset condition is: according to the first working parameter and the second working parameter, the calibration coefficient of the ultrasonic device 100 exceeds the preset threshold value. This implementation mode determines whether the ultrasonic device 100 varies by the calibration coefficient, which is easy to implement. Of course, in specific applications, as an alternative implementation mode, other methods can also be used to determine whether the ultrasonic device 100 varies.

[0102] As an embodiment, the above-mentioned control of the ultrasonic device 100 to transmit ultrasonic waves to the second reference medium, and obtaining the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium, includes: controlling the liquid transfer device to dispense the second reference medium into the empty reaction container 30, controlling the reaction container 30 moving device to move the reaction container 30 loaded with the second reference medium to the ultrasonic mixing position, controlling the ultrasonic device 100 to perform ultrasonic work on the second reference medium located at the ultrasonic mixing position and located in the reaction container 30, and obtaining the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium. In this embodiment, the operation process of the ultrasonic device 100 under the second reference medium working condition is: first add the second reference medium to the empty reaction container 30, and then move the reaction container 30 loaded with the second reference medium to the ultrasonic mixing position for ultrasonic work, which is similar to the ultrasonic mixing process of the target liquid 20, except that the liquid added to the reaction container 30 and the object of ultrasonic mixing are different. Of course, in a specific application, as an alternative embodiment, the empty reaction container 30 can also be first moved to the ultrasonic mixing position, and then the second reference medium can be dispensed into the empty reaction container 30 at the ultrasonic mixing position.

[0103] Reference Figure 1 , Figure 2 and Figure 3 As shown, as an embodiment, the sample analyzer 10 further includes a sample dispensing device 300, which is used to draw a sample from a sample container and distribute at least part of the drawn sample to a reaction container 30. The sample container is used to load a sample collected from a patient. The reaction container 30 is used to carry the sample for reaction and / or measurement. The sample dispensing device 300 is mainly used to achieve quantitative distribution of the sample.

[0104] As an implementation manner, the sample distributing device 300 is used to draw samples from a sample container located at a sample aspirating position and distribute them to a reaction container 30 located at a sample adding position.

[0105] As an embodiment, the sample dispensing device 300 includes a sample needle, a first motion driving device and a first suction and discharge driving device, wherein the first motion driving device is used to drive the sample needle to move in two-dimensional or three-dimensional space so as to move the sample needle to different positions, such as a standby position, a sample suction position, a sample addition position, a cleaning position, etc. The first suction and discharge driving device is used to drive the sample needle to perform suction and discharge actions.

[0106] As an embodiment, the sample analyzer 10 further includes a reagent dispensing device 400, which is used to absorb reagents from a reagent container and distribute at least part of the absorbed reagents to the reaction container 30. The reagent container is used to store reagents required for the assay. The reagent dispensing device 400 is mainly used to achieve quantitative distribution of reagents.

[0107] As an embodiment, the reagent dispensing device 400 includes a reagent needle, a second motion drive device and a second suction and discharge drive device, and the second motion drive device is used to drive the reagent needle to move in two-dimensional or three-dimensional space, so that the reagent needle moves to different workstations, such as a standby position, a reagent suction position, a reagent addition position, a cleaning position, etc. The second suction and discharge drive device is used to drive the reagent needle to perform suction and discharge actions. It should be noted that the reagent dispensing device 400 may include multiple reagent needles, multiple groups of second motion drive devices and multiple groups of second suction and discharge drive devices, and any two reagent needles can be used to absorb and dispense different types of reagents, respectively, or can be used to absorb and dispense the same type of reagent at different times.

[0108] As an embodiment, the sample analyzer 10 further includes a reagent loading device 600 for storing reagent containers. The reagent dispensing device 400 is used to absorb reagents from the reagent containers on the reagent loading device 600 and distribute at least part of the absorbed reagents into the reaction container 30.

[0109] As an implementation manner, the reagent loading device 600 is in the shape of a disk, and a plurality of reagent positions are formed thereon, and a plurality of reagent containers can be stored simultaneously.

[0110] As an embodiment, the pipetting device is a reagent dispensing device 400, that is, the reagent dispensing device 400 is reused to dispense the reagent and the second reference medium. In this embodiment, the multiplexed reagent dispensing device 400 dispenses the second reference medium in the calibration operation of the ultrasonic device 100, so that the calibration operation of the ultrasonic device 100 does not require the additional installation of other devices to dispense the second reference medium, and its structure is simple. Of course, in specific applications, the setting method of the pipetting device is not limited to this. For example, as an alternative embodiment, the pipetting device is a sample dispensing device 300, that is, the sample dispensing device 300 can be reused to dispense the sample and the second reference medium; or, as another alternative embodiment, the pipetting device is a device independent of the reagent dispensing device 400 and the sample dispensing device 300, that is, the pipetting device may not reuse the reagent dispensing device 400 and the sample dispensing device 300.

[0111] As an embodiment, the sample analyzer 10 further includes a measuring device 500, which is used to measure the reaction solution made of at least the mixed solution. The measuring device 500 is used to measure the reaction solution in the reaction container 30. The measuring device 500 may be an optical measuring device 500.

[0112] As an embodiment, the sample analyzer 10 further includes an incubation device 700 and a transfer device 800. The incubation device 700 is used to incubate the liquid in the reaction container 30. The transfer device 800 is used to transfer the reaction container 30, for example, to transfer the reaction container 30 after sample addition to the incubation device 700. The reagent dispensing device 400 is used to dispense the reagent into the reaction container 30 located on the incubation device 700. The measuring device 500 is arranged beside the incubation device 700, so as to perform optical measurement on the reaction liquid in the reaction container 30 on the incubation device 700.

[0113] As an embodiment, the incubation device 700 is disc-shaped and has a plurality of incubation holes formed thereon, so that a plurality of reaction containers 30 can be incubated simultaneously.

[0114] As an embodiment, the incubation device 700 is a disc-shaped structure that can drive the reaction container 30 to rotate.

[0115] As an embodiment, the ultrasonic mixing station is located on the incubation device 700. The ultrasonic device 100 is used to ultrasonically mix the target liquid 20 in the reaction container 30 on the incubation device 700. Of course, in a specific application, as an alternative embodiment, the ultrasonic mixing station may not be located on the incubation device 700.

[0116] As an embodiment, the transfer device 800 includes at least one clamping component and at least one third motion driving device for driving the clamping component to move.

[0117] As an embodiment, in the case where the pipetting device is a reagent dispensing device 400, the above-mentioned reaction container 30 moving device for moving the reaction container 30 loaded with the second reference medium to the ultrasonic mixing position is an incubation device 700, that is: in the calibration operation of the ultrasonic device 100, after the reagent dispensing device 400 dispenses the second reference medium into the reaction container 30 on the incubation device 700, the reaction container 30 loaded with the second reference medium is moved from the reagent adding position to the ultrasonic mixing position by the rotation of the incubation device 700.

[0118] In the case where the pipetting device is a sample dispensing device, the reaction container 30 moving device for transferring the reaction container 30 loaded with the second reference medium to the ultrasonic mixing position is a combination of a transfer device 800 and an incubation device 700, that is, in the calibration operation of the ultrasonic device 100, after the sample dispensing device 300 dispenses the second reference medium into the reaction container 30 at the sample loading position, the transfer device 800 first moves the reaction container 30 loaded with the second reference medium from the sample loading position to the sample loading position of the incubation device 700, and then the incubation device 700 rotates to move the reaction container 30 loaded with the second reference medium from the sample loading position to the ultrasonic mixing position.

[0119] As an embodiment, the sample analyzer 10 also includes a reaction container providing device 900 and a reaction container 30 recovery device, the reaction container providing device 900 is used to provide the reaction container 30, and the reaction container 30 recovery device is used to recover the reaction container 30. The sample analyzer 10 also forms a recovery position, and the reaction container 30 recovery device is located below the recovery position. The transfer device 800 is also used to transfer the reaction container 30 from the incubation device 700 to the recovery position for release and recovery. In this embodiment, the reaction container 30 is a disposable container, that is, a reaction container 30 is recycled after completing a measurement project. Of course, in specific applications, the reaction container 30 can also be a recycled container, that is, the reaction container 30 completes a measurement project, can be cleaned in the sample analyzer 10 and then reused for other measurement projects.

[0120] As an embodiment, the sample analyzer 10 also includes a sample management device 101 and a sample transport device 102. The sample management device 101 is used to place a sample container loaded with a sample to achieve sample loading and storage. The sample transport device 102 is used to transport the sample container output by the sample management device 101 to the sample suction position.

[0121] Reference Figure 1 , Figure 2 and Figure 5 As shown, as an embodiment, the control device 200 includes an ultrasonic control board 210 and a main control board 220. The ultrasonic control board 210 is used to control the operation of the ultrasonic device 100, and the main control board 220 is at least used to control the operation of the ultrasonic control board 210, the sample dispensing device 300, the reagent dispensing device 400 and the measuring device 500. In this embodiment, the ultrasonic control board 210 and the main control board 220 are two independent circuit boards. Of course, in a specific application, as an alternative embodiment, the ultrasonic control board 210 and the main control board 220 can also be integrated into one circuit board.

[0122] As an embodiment, the main control board 220 is also used to control the operation of the incubation device 700 , the transfer device 800 , the magnetic separation device 103 , the reaction container providing device 900 , the sample management device 101 and the sample transport device 102 .

[0123] As an embodiment, the sample is a blood sample, the sample analyzer 10 is an immunoanalyzer, and the sample analyzer 10 further includes a magnetic separation device 103, which is used to magnetically separate the liquid in the reaction container 30. The liquid treated by the magnetic separation device 103 includes a clear liquid and a magnetic bead liquid, the clear liquid is treated as a waste liquid, and the magnetic bead liquid is used to prepare a subsequent reaction liquid. The measurement process of a measurement item in the immunoanalyzer includes: adding a sample, adding a reagent to form a first mixed liquid, mixing and incubating the first mixed liquid to obtain a second mixed liquid, magnetically separating the second mixed liquid to obtain a liquid after magnetic separation, adding a substrate to the liquid after magnetic separation to obtain a third mixed liquid, mixing and incubating the third mixed liquid to obtain a reaction liquid, and optically measuring the reaction liquid. During the magnetic separation process, the operation of adding a separation liquid and mixing will be performed. The above-mentioned mixed liquid as the target liquid includes at least one of the following situations: a first mixed liquid after adding a sample and a reagent and before incubation, a second mixed liquid during the magnetic separation process, and a third mixed liquid formed after the magnetic separation and the addition of a substrate and before incubation. Of course, in a specific application, the type of the sample analyzer 10 is not limited thereto. For example, as an alternative embodiment, the sample analyzer 10 may also be a biochemical analyzer, a blood routine cell analyzer, or a coagulation analyzer.

[0124] This embodiment also provides a control method for the ultrasonic device 100 in the sample analyzer 10, which includes the following steps: controlling the ultrasonic device 100 to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; controlling the ultrasonic device 100 to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; obtaining a calibration coefficient of the ultrasonic device 100 at least based on the first working parameter and the second working parameter; determining a target parameter based on the calibration coefficient, and driving the ultrasonic device 100 to work with the target parameter to ultrasonically mix the target liquid 20; wherein the first reference medium and the second reference medium are two different media whose physical properties and / or composition are different from those of the target liquid 20. In this embodiment, the control method of the ultrasonic device 100 in the sample analyzer 10 is similar to the control principle described in the above-mentioned sample analyzer 10, and will not be described in detail here.

[0125] As an embodiment, in the control method of the ultrasonic device 100 in the sample analyzer 10, the above-mentioned acquisition of the first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium includes: acquiring the first working voltage and the first working current when the ultrasonic device 100 satisfies the first preset condition in the process of transmitting ultrasonic waves to the first reference medium. The above-mentioned acquisition of the second working parameter of the ultrasonic device 100 includes: acquiring the second working voltage and the second working current when the ultrasonic device 100 satisfies the first preset condition in the process of transmitting ultrasonic waves to the second reference medium. Among them, the first preset condition includes: the driving frequency of the ultrasonic device 100 reaches the first preset range of the resonant frequency, and / or the phase difference between the working voltage and the working current of the ultrasonic device 100 is within the second preset range.

[0126] As an embodiment, the control method of the ultrasonic device 100 in the sample analyzer 10, before controlling the ultrasonic device 100 to transmit ultrasonic waves to a first reference medium and obtaining a first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; and controlling the ultrasonic device 100 to transmit ultrasonic waves to a second reference medium and obtaining a second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium, further includes: obtaining a second standard parameter of the calibrated ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium. The method of obtaining the calibration coefficient of the ultrasonic device 100 based on at least the first working parameter and the second working parameter includes: obtaining the calibration coefficient of the ultrasonic device 100 based on the first working parameter, the second working parameter and the second standard parameter.

[0127] As an implementation manner, the second standard parameter is the mechanical load impedance generated by the ultrasonic device 100 transmitting ultrasonic waves to the second reference medium.

[0128] As an embodiment, in the control method of the ultrasonic device 100 in the sample analyzer 10, the above-mentioned control of the ultrasonic device 100 to transmit ultrasonic waves to the second reference medium and obtaining the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium include: controlling the pipetting device to dispense the second reference medium into the empty reaction container 30, controlling the reaction container 30 moving device to move the reaction container 30 loaded with the second reference medium to the ultrasonic mixing position, controlling the ultrasonic device 100 to perform ultrasonic work on the second reference medium located at the ultrasonic mixing position and located in the reaction container 30, and obtaining the second working parameter of the ultrasonic device 100; the pipetting device is: a reagent dispensing device 400, or a sample dispensing device 300, or a device independent of the reagent dispensing device 400 and the sample dispensing device 300.

[0129] As an embodiment, in the control method of the ultrasonic device 100 in the sample analyzer 10, the calibration coefficient includes the electromechanical conversion efficiency of the ultrasonic device 100; determining the target parameter according to the calibration coefficient includes: determining the target parameter according to the calibration coefficient and the preset vibration velocity of the ultrasonic device 100.

[0130] The specific principles and implementations of the control method provided in this embodiment are similar to those described in the above-mentioned sample analyzer 10 and will not be described in detail here.

[0131] This embodiment also provides a computer-readable storage medium, which stores a computer program. When the computer program is executed by a processor (e.g., the processor in the control device 200 described above), the processor implements the steps of the control method of the ultrasonic device 100 in the sample analyzer 10 described above. The computer-readable storage medium may be an internal storage unit of the sample analyzer 10 described above, such as a hard disk or memory of the sample analyzer 10; or, the computer-readable storage medium may also be an external storage device of the sample analyzer 10, such as a plug-in hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (Secure Digital, SD) card, a flash card (FlashCard), etc., equipped on the sample analyzer 10.

[0132] In this embodiment, the online calibration of the ultrasonic device 100 in the sample analyzer 10 is implemented through program settings, and the online calibration problem of the sample analyzer 10 (especially the sample analyzer 10 with narrow working space and complex working environment) integrated with the piezoelectric ultrasonic device 100 is solved without introducing additional sensors.

[0133] As an implementation, the online calibration principle of the ultrasonic device 100 in the sample analyzer 10 includes the following:

[0134] 1) The electromechanical equivalent model of the piezoelectric ultrasonic device 100 is as follows: Figure 4As shown, U is the driving voltage (also the working voltage of the ultrasonic device 100) input by the ultrasonic control board 210 to the ultrasonic device 100; C0 is the static capacitance of the ultrasonic device 100; L1 is the dynamic inductance of the ultrasonic device 100 (i.e., the equivalent inductance of the ultrasonic device 100 when it is unloaded); C1 is the dynamic capacitance of the ultrasonic device 100 (i.e., the equivalent capacitance of the ultrasonic device 100 when it is unloaded); R1 is the dynamic resistance of the ultrasonic device 100 (i.e., the equivalent resistance of the ultrasonic device 100 when it is unloaded); Z1 is the dynamic impedance of the ultrasonic device 100 (i.e., the equivalent impedance of the ultrasonic device 100 when it is unloaded); L2 is the equivalent load inductance acting on the ultrasonic device 100; C2 is the equivalent load capacitance acting on the ultrasonic device 100; R2 is the equivalent load resistance acting on the ultrasonic device 100; and Z2 is the equivalent load impedance acting on the ultrasonic device 100. When the frequency of the driving voltage U is the same as the resonant frequency of the dynamic branch determined by L1 and C1, the no-load dynamic branch is purely resistive; at the same time, the impedance of the static branch determined by C0 under the driving voltage U is large enough to make R1≈U / I, where U and I are the amplitudes of the voltage and current at both ends of the ultrasonic device 100, respectively.

[0135] 2) The resonant frequency fs can be obtained by the integrated frequency sweep function of the ultrasonic control board 210. By adjusting the frequency f of the driving voltage U of the ultrasonic device 100 and collecting the corresponding current I, the relationship between the impedance amplitude U / I and the driving frequency f is obtained. When U / I is the smallest, f is approximately equal to the resonant frequency fs.

[0136] 3) When the ultrasonic device 100 is working, the end face of the ultrasonic vibrator 120 (such as a transformer) radiates sound waves to the contacting medium, and the load impedance of the ultrasonic device 100 is equivalent to Z2. When the object of action is air or cavitated liquid, the load has little effect on the resonant frequency and is approximately resistive, that is, Z2≈R2.

[0137] 4) The equivalent electrical load Z2 generated by the ultrasonic device 100 acting on the medium depends on the mechanical load impedance Zs and the electromechanical conversion efficiency N of the ultrasonic device 100. The mechanical load impedance Zs is determined by the reaction force F acting on the end face of the ultrasonic device 100 and the vibration velocity V of the ultrasonic device 100, that is, Zs = F / V. The electromechanical conversion efficiency N is determined by the dynamic branch current I 负载 and I 负载 The vibration velocity V of the ultrasonic device 100 under the action of the load is determined by the force F applied to the ultrasonic device 100 and the load equivalent voltage U 负载 Determine, that is, N = V / I 负载 =U 负载 / F; According to the formula Zs=F / V and N=V / I 负载 =U 负载 / F, we can get Z2=N 2 Zs.

[0138] 5) The mechanical load impedance Zs depends on the properties of the object (density, viscosity, sound speed, etc.) and the boundary conditions of the acoustic field (container shape, material, liquid volume, relative position, etc.); for the same container and the same liquid (such as purified water), the ultrasonic device 100 outputs ultrasonic vibrations at the same position, and the mechanical load impedance Zs received can be considered to be the same, thereby constructing a fixed mechanical load condition;

[0139] 6) Under the target driving voltage of the dynamic branch resonant frequency, the impedance of the static branch is large enough, and the influence of the static branch can be ignored. The ultrasonic device 100 can be calibrated by using the working voltage U and working current I of the ultrasonic device 100 measured under no-load and fixed mechanical load conditions. Specifically, under the target driving voltage of the dynamic branch resonant frequency, for the no-load condition, U 空载 / I 空载 =R 1当前 ; For fixed mechanical load Zs, there are: U 负载 / I 负载 =R 1当前 +N 当前 2 Zs, from which we can get N 当前 2 =(U 负载 / I 负载 -R 1当前 ) / Zs. Among them, U 空载 ,I 空载 , U 负载 ,I 负载 is the working voltage and working current of the ultrasonic device 100 under different working conditions, which can be directly measured by the integrated voltage and current sampling module of the ultrasonic control board 210; Zs can be first measured by the calibrated ultrasonic device 100 under a fixed mechanical load condition (such as the working condition of the second reference medium mentioned above) and used as a fixed parameter. Thus, the current equivalent no-load impedance R of the ultrasonic device 100 can be obtained. 1当前 And the current electromechanical conversion coefficient N 当前 .

[0140] 6) Obtain the current electromechanical conversion coefficient N of the ultrasonic device 100 当前 and the equivalent no-load impedance R 1当前 , the response characteristics of the ultrasonic device 100 can be obtained, that is, the calibration of the ultrasonic device 100 has been achieved. Then, according to the electromechanical conversion relationship V 目标 =N 当前 I 目标 According to the actual use scenario, the target driving current I corresponding to the required vibration velocity V output by the ultrasonic device 100 is determined. 目标, or further combine the resonant frequency fs to determine the parameters related to the vibration speed, such as amplitude, sound pressure, sound intensity, etc. to achieve the target driving current I corresponding to the specified value 目标 , or by the target drive current I 目标 The target driving voltage and the target driving power are calculated.

[0141] 7) This solution calibrates the impedance monitoring of the ultrasonic device 100 , which can correct the influence of the variation of the ultrasonic device 100 on the no-load impedance monitoring, and does not require the introduction of additional sensors on the sample analyzer 10 integrated with the ultrasonic device 100 .

[0142] By adopting the above principle, in the calibration and variation monitoring implementation scheme of the ultrasonic device 100, there is no need to add additional sensors and measuring equipment. This is particularly suitable for equipment and products that require online and on-machine energy calibration of the integrated ultrasonic device 100. This calibration scheme has significant advantages in cost, integration space, and ease of integration.

[0143] Embodiment 2:

[0144] The sample analyzer 10 and the control method of the ultrasonic device 100 in the sample analyzer 10 provided by the present invention are different from those in the first embodiment mainly in that the setting function of the ultrasonic device 100 is different, which is specifically reflected in that: in the first embodiment, the ultrasonic device 100 is used to ultrasonically mix the target liquid 20; while in the present embodiment, the ultrasonic device 100 is used to transmit ultrasonic waves to the target liquid 20 to clean the parts to be cleaned.

[0145] As an implementation mode, the sample analyzer 10 provided in this embodiment includes a sample dispensing device 300, a reagent dispensing device 400, a cleaning liquid supply device, an ultrasonic device 100, a measuring device 500 and a control device 200. The sample dispensing device 300 includes a sample needle, which is used to draw a sample from a sample container and distribute at least a portion of the drawn sample to a reaction container 30. The reagent dispensing device 400 includes a reagent needle, which is used to draw a reagent from a reagent container and distribute at least a portion of the drawn reagent to a reaction container 30. The cleaning liquid supply device is used to supply a cleaning liquid to clean one of the sample needle, the reagent needle, the reaction container 30 and the stirring rod. The ultrasonic device 100 is used to transmit ultrasonic waves to a target liquid 20, and the target liquid 20 is a cleaning liquid. The measuring device 500 is used to measure a reaction liquid made of at least a mixed liquid. The control device 200 is configured to control the ultrasonic device 100 to perform the following calibration operations: control the ultrasonic device 100 to transmit ultrasonic waves to the first reference medium, and obtain the first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device 100 to transmit ultrasonic waves to the second reference medium, and obtain the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; obtain the calibration coefficient of the ultrasonic device 100 at least based on the first working parameter and the second working parameter. The control device 200 is also configured to control the ultrasonic device 100 to perform the following ultrasonic work: determine the target parameter according to the calibration coefficient, and drive the ultrasonic device 100 to work with the target parameter to transmit ultrasonic waves to the target liquid 20. In this embodiment, when the ultrasonic device 100 is used for ultrasonic cleaning, in the calibration and variation monitoring implementation scheme of the ultrasonic device 100, there is no need to add additional sensors and measuring equipment.

[0146] As an embodiment, when the ultrasonic device 100 is used to clean the sample needle, the sample analyzer 10 further includes a first cleaning tank, and the cleaning liquid supply device is used to supply cleaning liquid to the first cleaning tank to clean the sample needle. The ultrasonic device 100 includes an ultrasonic transducer 110 and an ultrasonic vibrator 120, and the ultrasonic vibrator 120 is a first ultrasonic seat arranged outside the first cleaning tank, and the first ultrasonic seat is used to transmit ultrasonic waves from outside the first cleaning tank to the cleaning liquid in the first cleaning tank to clean the sample needle inserted into the first cleaning tank.

[0147] As an embodiment, when the ultrasonic device 100 is used to clean the reagent needle, the sample analyzer 10 also includes a second cleaning tank, and the cleaning liquid supply device is used to supply cleaning liquid to the second cleaning tank to clean the reagent needle. The ultrasonic device 100 includes an ultrasonic transducer 110 and an ultrasonic vibrator 120, and the ultrasonic vibrator 120 is a second ultrasonic seat arranged outside the second cleaning tank. The second ultrasonic seat is used to transmit ultrasonic waves from outside the second cleaning tank to the cleaning liquid in the second cleaning tank to clean the reagent needle inserted into the second cleaning tank.

[0148] As an embodiment, when the ultrasonic device 100 is used to clean the stirring rod, the stirring rod is used to stir the sample or reagent or the mixture of the sample and the reagent in the reaction container 30. The sample analyzer 10 also includes a third cleaning tank, and the cleaning liquid supply device is used to supply cleaning liquid to the third cleaning tank to clean the stirring rod. The ultrasonic device 100 includes an ultrasonic transducer 110 and an ultrasonic vibrator 120. The ultrasonic vibrator 120 is a third ultrasonic seat provided outside the third cleaning tank. The third ultrasonic seat is used to transmit ultrasonic waves from outside the third cleaning tank to the cleaning liquid in the third cleaning tank to clean the stirring rod inserted into the third cleaning tank.

[0149] As an embodiment, when the ultrasonic device 100 is used to clean the reaction container 30, the cleaning liquid supply device is used to supply the cleaning liquid to the reaction container 30 to clean the reaction container 30. The ultrasonic device 100 includes an ultrasonic transducer 110 and an ultrasonic vibrator 120, the ultrasonic vibrator 120 is an ultrasonic vibration rod inserted into the reaction container 30 or a fourth ultrasonic seat provided outside the reaction container 30, and the ultrasonic vibrator 120 is used to transmit ultrasonic waves to the cleaning liquid in the reaction container 30 to clean the reaction container 30.

[0150] The control method of the ultrasonic device 100 in the sample analyzer 10 provided in the present embodiment includes the following steps: controlling the ultrasonic device 100 to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; controlling the ultrasonic device 100 to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; obtaining a calibration coefficient of the ultrasonic device 100 based on at least the first working parameter and the second working parameter; determining a target parameter based on the calibration coefficient, and driving the ultrasonic device 100 to work with the target parameter to transmit ultrasonic waves to the target liquid 20.

[0151] Except for the above differences, other parts of the sample analyzer 10 and the control method of the ultrasonic device 100 in the sample analyzer 10 provided in this embodiment can refer to the first embodiment and will not be described in detail here.

[0152] Embodiment three:

[0153] The sample analyzer 10 and the control method of the ultrasonic device 100 in the sample analyzer 10 provided in this embodiment are different from those in the first embodiment mainly in that the purposes of the first working parameter and the second working parameter are different. Specifically, in the first embodiment, the first working parameter and the second working parameter are mainly used to calibrate the ultrasonic device 100; while in this embodiment, the first working parameter and the second working parameter are mainly used to determine the variation state of the ultrasonic device 100.

[0154] As an implementation mode, the sample analyzer 10 provided in this embodiment includes a sample dispensing device 300, a reagent dispensing device 400, an ultrasonic device 100, a measuring device 500 and a control device 200. The sample dispensing device 300 is used to absorb a sample from a sample container and distribute at least a portion of the absorbed sample to a reaction container 30; the reagent dispensing device 400 is used to absorb a reagent from a reagent container and distribute at least a portion of the absorbed reagent to the reaction container 30; the ultrasonic device 100 is used to perform ultrasonic work on a target liquid 20, and the target liquid 20 is one of a sample, a reagent, a cleaning liquid and a mixed liquid containing at least a sample and a reagent; the measuring device 500 is used to perform ultrasonic work on a target liquid 20, and the target liquid 20 is one of a sample, a reagent, a cleaning liquid and a mixed liquid containing at least a sample and a reagent; 00 is used to measure the reaction liquid made of at least a mixed liquid; the control device 200 is configured to: control the ultrasonic device 100 to transmit ultrasonic waves to the first reference medium, and obtain the first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device 100 to transmit ultrasonic waves to the second reference medium, and obtain the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; determine the variation state of the ultrasonic device 100 at least according to the first working parameter and the second working parameter; wherein the first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid 20. The variation state may include whether the ultrasonic device 100 varies. This embodiment can realize the confirmation of the variation state of the ultrasonic device 100. Since the variation state of the ultrasonic device 100 in this embodiment is confirmed by using the inherent device of the sample analyzer 10 to realize on-machine detection, no other measuring equipment needs to be introduced, so it has significant advantages in cost, integration space, and ease of integration.

[0155] As an embodiment, the control device 200 is further configured to: output a warning signal when it is determined that the variation state of the ultrasonic device 100 meets the third preset condition. The warning signal is mainly used to prompt the operator to replace the ultrasonic device 100 when the ultrasonic device 100 varies. The warning signal includes at least one of the following forms: a prompt on the display screen, a sound warning signal, and a light warning signal.

[0156] As an implementation mode, the third preset condition is: according to the first working parameter and the second working parameter, the calibration coefficient of the ultrasonic device 100 exceeds the preset threshold value. The calibration coefficient determination method of the ultrasonic device 100 can refer to the first embodiment. This implementation mode determines whether the ultrasonic device 100 varies by the calibration coefficient. Of course, in a specific application, as an alternative implementation mode, other methods can also be used to determine whether the ultrasonic device 100 varies.

[0157] As an embodiment, the first reference medium is air, and the second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components.

[0158] As an embodiment, the above-mentioned acquisition of the first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium includes: acquiring the first working voltage and the first working current of the ultrasonic device 100 when the first preset condition is satisfied during the process of transmitting ultrasonic waves to the first reference medium; the above-mentioned acquisition of the second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium includes: acquiring the second working voltage and the second working current of the ultrasonic device 100 when the first preset condition is satisfied during the process of transmitting ultrasonic waves to the second reference medium; wherein the first preset condition includes: the driving frequency of the ultrasonic device 100 reaches within a first preset range of the resonant frequency, and / or the phase difference between the working voltage and the working current of the ultrasonic device 100 is within a second preset range.

[0159] As an embodiment, the control device 200 is also configured to: before controlling the ultrasonic device 100 to perform a calibration operation, obtain a first standard parameter in a process in which the calibrated ultrasonic device 100 transmits ultrasonic waves to a first reference medium, and / or obtain a second standard parameter in a process in which the calibrated ultrasonic device 100 transmits ultrasonic waves to a second reference medium; determine a variation state of the ultrasonic device 100 based at least on the first working parameter and the second working parameter, including: determining the variation state of the ultrasonic device 100 based on the first working parameter, the second working parameter, and the first standard parameter and / or the second standard parameter.

[0160] The control method of the ultrasonic device 100 in the sample analyzer 10 provided in this embodiment includes the following steps: controlling the ultrasonic device 100 to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the first reference medium; controlling the ultrasonic device 100 to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device 100 in the process of transmitting ultrasonic waves to the second reference medium; determining a variation state of the ultrasonic device 100 based on at least the first working parameter and the second working parameter; wherein the first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid 20.

[0161] Except for the above differences, other parts of the sample analyzer 10 and the control method of the ultrasonic device 100 in the sample analyzer 10 provided in this embodiment can refer to the first embodiment or the second embodiment, and will not be described in detail here.

[0162] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A sample analyzer, characterized in that: include: A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and distribute at least a portion of the drawn sample to a reaction container; A reagent dispensing device, the reagent dispensing device is used to suck the reagent from the reagent container and distribute at least part of the sucked reagent to the reaction container; An ultrasonic device, the ultrasonic device is used to ultrasonically mix a target liquid, the target liquid being one of the sample, the reagent, and a mixed liquid containing at least the sample and the reagent; a measuring device for measuring a reaction solution made of at least a mixed solution; A control device, the control device is configured to control the ultrasonic device to perform the following calibration operations: control the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtain a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtain a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; obtain a calibration coefficient of the ultrasonic device based on at least the first working parameter and the second working parameter; The control device is further configured to control the ultrasonic device to perform the following ultrasonic mixing operation: determining a target parameter according to the calibration coefficient, and driving the ultrasonic device to operate with the target parameter to ultrasonically mix the target liquid; The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

2. The sample analyzer according to claim 1, wherein: The obtaining of the first operating parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium comprises: obtaining a first operating voltage and a first operating current when the ultrasonic device satisfies a first preset condition in the process of transmitting ultrasonic waves to the first reference medium; The obtaining of the second operating parameter of the ultrasonic device during the process of the ultrasonic device transmitting the ultrasonic wave to the second reference medium comprises: obtaining a second operating voltage and a second operating current when the ultrasonic device satisfies the first preset condition during the process of the ultrasonic device transmitting the ultrasonic wave to the second reference medium; Wherein, the first preset condition includes: the driving frequency of the ultrasonic device is within a first preset range of the resonant frequency, and / or the phase difference between the working voltage and the working current of the ultrasonic device is within a second preset range.

3. The sample analyzer according to claim 1, wherein: The first reference medium is air; and / or, The second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components.

4. The sample analyzer according to claim 1, wherein: The control device is further configured to: before controlling the ultrasonic device to perform the calibration operation, obtain a first standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium, and / or obtain a second standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; The obtaining of the calibration coefficient of the ultrasonic device at least based on the first working parameter and the second working parameter includes: obtaining the calibration coefficient of the ultrasonic device based on the first working parameter, the second working parameter, and the first standard parameter and / or the second standard parameter.

5. The sample analyzer according to claim 4, characterized in that: The first reference medium is air, and the control device is configured to: before controlling the ultrasonic device to perform the calibration operation, obtain a second standard parameter when the calibrated ultrasonic device transmits ultrasonic waves to the second reference medium; The obtaining of the calibration coefficient of the ultrasonic device at least according to the first working parameter and the second working parameter comprises: obtaining the calibration coefficient of the ultrasonic device according to the first working parameter, the second working parameter and the second standard parameter; and / or, The second standard parameter is the mechanical load impedance generated by the calibrated ultrasonic device transmitting ultrasonic waves to the second reference medium.

6. The sample analyzer according to claim 4 or 5, characterized in that: The second standard parameter is a pre-stored value pre-stored in the sample analyzer; or, The control device is also configured to determine the second standard parameter in the following manner: obtaining a third working parameter of the calibrated ultrasonic device transmitting ultrasonic waves under no-load conditions; controlling the calibrated ultrasonic device to transmit ultrasonic waves to the second reference medium, and obtaining a fourth working parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; and obtaining the second standard parameter based on the third working parameter and the fourth working parameter.

7. The sample analyzer according to any one of claims 1 to 5, characterized in that: The ultrasonic device is used to ultrasonically mix the target liquid located at the ultrasonic mixing position and in the reaction container; The second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components; The controlling the ultrasonic device to transmit ultrasonic waves to the second reference medium and obtaining the second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium comprises: controlling the liquid transfer device to dispense the second reference medium into an empty reaction container, controlling the reaction container moving device to move the reaction container loaded with the second reference medium to the ultrasonic mixing position, controlling the ultrasonic device to perform ultrasonic work on the second reference medium located at the ultrasonic mixing position and in the reaction container, and obtaining the second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; The pipetting device is: the reagent dispensing device, or the sample dispensing device, or a device independent of the reagent dispensing device and the sample dispensing device.

8. The sample analyzer according to any one of claims 1 to 5, characterized in that: The calibration coefficient includes the electromechanical conversion efficiency of the ultrasonic device; determining the target parameter according to the calibration coefficient includes: determining the target parameter according to the calibration coefficient and a preset vibration speed of the ultrasonic device; and / or, The ultrasonic device includes an ultrasonic transducer, an ultrasonic vibrator and a driving mechanism. The ultrasonic vibrator is used to ultrasonically mix the target liquid under the drive of the ultrasonic transducer, and the driving mechanism is used to drive the ultrasonic vibrator to move. The control device is also configured to: before driving the ultrasonic device to work with the target parameters, control the driving mechanism to drive the ultrasonic vibrator to insert into the target liquid.

9. The sample analyzer according to any one of claims 1 to 5, characterized in that: The calibration coefficient of the ultrasonic device is obtained at least based on the first working parameter and the second working parameter, including: obtaining a first impedance value formed by the first reference medium to the ultrasonic device according to the first working parameter, obtaining a second impedance value formed by the second reference medium to the ultrasonic device according to the second working parameter, and obtaining the calibration coefficient of the ultrasonic device at least based on the first impedance value and the second impedance value.

10. The sample analyzer according to any one of claims 1 to 5, characterized in that: The sample analyzer pre-stores at least one of the following second preset conditions: the duration of the ultrasonic device continuously performing the ultrasonic mixing operation reaches a first preset duration, the cumulative duration of the ultrasonic device performing the ultrasonic mixing operation since the last calibration operation reaches a second preset duration, the number of times the ultrasonic device continuously performs the ultrasonic mixing operation reaches a first preset number of times, the cumulative number of times the ultrasonic device performs the ultrasonic mixing operation since the last calibration operation reaches a second preset number of times, the first preset time point after the sample analyzer is turned on is reached, the second preset time point before the sample analyzer is turned off is reached, and the third preset time length has been reached since the last calibration operation; replacing the ultrasonic device; Arriving at a third preset time point of a preset period, wherein the preset period is every day, every two days, every three days, every four days, every five days, every week, every two weeks, every half month, every month, every two months, every three months, or every half year; The control device is further configured to: when any one of the second preset conditions is met, control the ultrasonic device to perform the calibration operation.

11. The sample analyzer according to any one of claims 1 to 5, characterized in that: The control device is further configured to determine a variation state of the ultrasound device based at least on the first operating parameter and the second operating parameter.

12. The sample analyzer according to claim 11, characterized in that: The control device is further configured to output a warning signal when it is determined that the variation state of the ultrasonic device meets a third preset condition.

13. A sample analyzer, characterized in that: include: A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and distribute at least a portion of the drawn sample to a reaction container; A reagent dispensing device, the reagent dispensing device is used to suck the reagent from the reagent container and distribute at least part of the sucked reagent to the reaction container; An ultrasonic device, the ultrasonic device is used to perform ultrasonic work on a target liquid, the target liquid is one of the sample, the reagent, a cleaning solution, and a mixed solution containing at least the sample and the reagent; a measuring device for measuring a reaction solution made of at least a mixed solution; A control device, wherein the control device is configured to: control the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtain a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium; control the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtain a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; and determine a variation state of the ultrasonic device based on at least the first working parameter and the second working parameter; The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

14. The sample analyzer according to claim 13, wherein: The control device is further configured to: output a warning signal when it is determined that the variation state of the ultrasonic device meets a third preset condition; and / or, The first reference medium is air, and the second reference medium is purified water, or the second reference medium is a reference liquid different from purified water and having known physical properties and / or known components.

15. The sample analyzer according to claim 13 or 14, characterized in that: The obtaining of the first operating parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium comprises: obtaining a first operating voltage and a first operating current when the ultrasonic device satisfies a first preset condition in the process of transmitting ultrasonic waves to the first reference medium; The obtaining of the second operating parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium comprises: obtaining a second operating voltage and a second operating current when the ultrasonic device satisfies the first preset condition in the process of transmitting ultrasonic waves to the second reference medium; Wherein, the first preset condition includes: the driving frequency of the ultrasonic device is within a first preset range of the resonant frequency, and / or the phase difference between the working voltage and the working current of the ultrasonic device is within a second preset range.

16. The sample analyzer according to claim 13 or 14, characterized in that: The control device is further configured to: before controlling the ultrasonic device to perform the calibration operation, obtain a first standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium, and / or obtain a second standard parameter of the calibrated ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; Determining the variation state of the ultrasonic device at least based on the first working parameter and the second working parameter includes: determining the variation state of the ultrasonic device based on the first working parameter, the second working parameter, and the first standard parameter and / or the second standard parameter.

17. A method for controlling an ultrasonic device in a sample analyzer, characterized in that: The steps include: Controlling the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium; Controlling the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; Obtaining a calibration coefficient of the ultrasonic device based at least on the first operating parameter and the second operating parameter; Determining a target parameter according to the calibration coefficient, and driving the ultrasonic device to operate with the target parameter to ultrasonically mix the target liquid; The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

18. A method for controlling an ultrasonic device in a sample analyzer, characterized in that: The steps include: Controlling the ultrasonic device to transmit ultrasonic waves to a first reference medium, and obtaining a first working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the first reference medium; Controlling the ultrasonic device to transmit ultrasonic waves to a second reference medium, and obtaining a second working parameter of the ultrasonic device in the process of transmitting ultrasonic waves to the second reference medium; determining a variation state of the ultrasonic device at least according to the first operating parameter and the second operating parameter; The first reference medium and the second reference medium are two different media whose physical properties and / or components are different from those of the target liquid.

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